ID   PTPS_CAEEL              Reviewed;         140 AA.
AC   O02058; Q2MGF4;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   10-OCT-2003, sequence version 2.
DT   16-JUN-2009, entry version 72.
DE   RecName: Full=Putative 6-pyruvoyl tetrahydrobiopterin synthase;
DE            Short=PTP synthase;
DE            Short=PTPS;
DE            EC=4.2.3.12;
GN   Name=ptps-1; ORFNames=B0041.6;
OS   Caenorhabditis elegans.
OC   Eukaryota; Metazoa; Nematoda; Chromadorea; Rhabditida; Rhabditoidea;
OC   Rhabditidae; Peloderinae; Caenorhabditis.
OX   NCBI_TaxID=6239;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND ALTERNATIVE
RP   SPLICING.
RC   STRAIN=Bristol N2;
RX   MEDLINE=99069613; PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG   The C. elegans sequencing consortium;
RT   "Genome sequence of the nematode C. elegans: a platform for
RT   investigating biology.";
RL   Science 282:2012-2018(1998).
CC   -!- FUNCTION: Involved in the biosynthesis of tetrahydrobiopterin, an
CC       essential cofactor of aromatic amino acid hydroxylases. Catalyzes
CC       the transformation of 7,8-dihydroneopterin triphosphate into 6-
CC       pyruvoyl tetrahydropterin (By similarity).
CC   -!- CATALYTIC ACTIVITY: 7,8-dihydroneopterin 3'-triphosphate = 6-
CC       pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate.
CC   -!- COFACTOR: Binds 1 zinc ion per subunit (By similarity).
CC   -!- PATHWAY: Cofactor biosynthesis; tetrahydrobiopterin biosynthesis;
CC       tetrahydrobiopterin from 2-amino-4-hydroxy-6-(erythro-1,2,3-
CC       trihydroxypropyl)-dihydropteridine triphosphate: step 1/3.
CC   -!- SUBUNIT: Homohexamer formed of two homotrimers in a head to head
CC       fashion (By similarity).
CC   -!- INTERACTION:
CC       Self; NbExp=2; IntAct=EBI-312091, EBI-312091;
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=a;
CC         IsoId=O02058-1; Sequence=Displayed;
CC         Note=No experimental confirmation available;
CC       Name=b;
CC         IsoId=O02058-2; Sequence=VSP_019291;
CC         Note=No experimental confirmation available;
CC   -!- MISCELLANEOUS: The active site is at the interface between 2
CC       subunits. The proton acceptor Cys is on one subunit, and the
CC       charge relay system is on the other subunit.
CC   -!- SIMILARITY: Belongs to the PTPS family.
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DR   EMBL; AF000196; AAC24255.2; -; Genomic_DNA.
DR   EMBL; AF000196; ABC71844.1; -; Genomic_DNA.
DR   PIR; T34033; T34033.
DR   RefSeq; NP_001040626.1; -.
DR   UniGene; Cel.23560; -.
DR   PDB; 2G64; X-ray; 1.80 A; A=1-139.
DR   PDBsum; 2G64; -.
DR   IntAct; O02058; 1.
DR   Ensembl; B0041.6; Caenorhabditis elegans.
DR   GeneID; 181823; -.
DR   KEGG; cel:B0041.6; -.
DR   NMPDR; fig|6239.3.peg.980; -.
DR   WormBase; WBGene00015010; ptps-1.
DR   WormPep; B0041.6a; CE34237.
DR   WormPep; B0041.6b; CE39541.
DR   OMA; O02058; AAHRLFN.
DR   BRENDA; 4.2.3.12; 672.
DR   NextBio; 915462; -.
DR   ArrayExpress; O02058; -.
DR   GO; GO:0003874; F:6-pyruvoyltetrahydropterin synthase activity; IEA:EC.
DR   GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0006729; P:tetrahydrobiopterin biosynthetic process; IEA:UniProtKB-KW.
DR   InterPro; IPR007116; 6-PTP_synth.
DR   InterPro; IPR007115; 6-PTP_synth-rel.
DR   PANTHER; PTHR12589; 6_PTP_synth; 1.
DR   Pfam; PF01242; PTPS; 1.
DR   TIGRFAMs; TIGR00039; 6PTHBS; 1.
DR   PROSITE; PS00987; PTPS_1; 1.
DR   PROSITE; PS00988; PTPS_2; FALSE_NEG.
PE   1: Evidence at protein level;
KW   3D-structure; Alternative splicing; Complete proteome; Lyase;
KW   Metal-binding; Tetrahydrobiopterin biosynthesis; Zinc.
FT   CHAIN         1    140       Putative 6-pyruvoyl tetrahydrobiopterin
FT                                synthase.
FT                                /FTId=PRO_0000057919.
FT   ACT_SITE     38     38       Proton acceptor (By similarity).
FT   ACT_SITE     84     84       Charge relay system (By similarity).
FT   ACT_SITE    129    129       Charge relay system (By similarity).
FT   METAL        19     19       Zinc (By similarity).
FT   METAL        44     44       Zinc (By similarity).
FT   METAL        46     46       Zinc (By similarity).
FT   VAR_SEQ       1      3       Missing (in isoform b).
FT                                /FTId=VSP_019291.
FT   STRAND        6     19
FT   HELIX        28     35
FT   HELIX        36     39
FT   STRAND       44     57
FT   TURN         60     62
FT   HELIX        68     80
FT   TURN         81     84
FT   HELIX        87     90
FT   HELIX        92     95
FT   HELIX       101    115
FT   HELIX       119    121
FT   STRAND      122    130
FT   STRAND      133    137
SQ   SEQUENCE   140 AA;  16046 MW;  7A677CE73A95882C CRC64;
     MFRMPIVTME RVDSFSAAHR LHSEKLSDAE NKETFGKCNN SNGHGHNYVW KVKLRGEVDP
     TSGMVYDLAK LKKEMSLVLD TVDHRNLDKD VEFFKTTVST SENVAIYMFE KLKSVMSNPS
     VLYKVTIEET PKNIFTYKGC
//