Putative 6-pyruvoyl tetrahydrobiopterin synthase

Contribute

Send feedback

Read comments (?) or add your own

O02058 (PTPS_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 93. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Putative 6-pyruvoyl tetrahydrobiopterin synthase
Short name=PTP synthase
Short name=PTPS
EC=4.2.3.12

Gene names

Name:	ptps-1
ORF Names:	B0041.6

Organism

Caenorhabditis elegans

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	140 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the biosynthesis of tetrahydrobiopterin, an essential cofactor of aromatic amino acid hydroxylases. Catalyzes the transformation of 7,8-dihydroneopterin triphosphate into 6-pyruvoyl tetrahydropterin By similarity.
Catalytic activity	7,8-dihydroneopterin 3'-triphosphate = 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	Cofactor biosynthesis; tetrahydrobiopterin biosynthesis; tetrahydrobiopterin from 7,8-dihydroneopterin triphosphate: step 1/3.
Subunit structure	Homohexamer formed of two homotrimers in a head to head fashion By similarity.
Miscellaneous	The active site is at the interface between 2 subunits. The proton acceptor Cys is on one subunit, and the charge relay system is on the other subunit.
Sequence similarities	Belongs to the PTPS family.

Ontologies

Keywords
Biological process	Tetrahydrobiopterin biosynthesis
Coding sequence diversity	Alternative splicing
Ligand	Metal-binding Zinc
Molecular function	Lyase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	tetrahydrobiopterin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	6-pyruvoyltetrahydropterin synthase activity Inferred from electronic annotation. Source: EC identical protein binding Inferred from physical interaction. Source: IntAct metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		4	EBI-312091,EBI-312091

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform a (identifier: O02058-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: No experimental confirmation available.

Isoform b (identifier: O02058-2) The sequence of this isoform differs from the canonical sequence as follows: 1-3: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 140

140

Putative 6-pyruvoyl tetrahydrobiopterin synthase

PRO_0000057919

Sites

Active site

Proton acceptor By similarity

Active site

Charge relay system By similarity

Active site

129

Charge relay system By similarity

Metal binding

Zinc By similarity

Metal binding

Zinc By similarity

Metal binding

Zinc By similarity

Natural variations

Alternative sequence

1 – 3

Missing in isoform b.

VSP_019291

Secondary structure

140

Helix Strand Turn

Details...

Sequences

Sequence		Length	Mass (Da)
Isoform a [UniParc]. Last modified October 10, 2003. Version 2. Checksum: 7A677CE73A95882C Show »	FASTA	140	16,046
10 20 30 40 50 60 MFRMPIVTME RVDSFSAAHR LHSEKLSDAE NKETFGKCNN SNGHGHNYVW KVKLRGEVDP 70 80 90 100 110 120 TSGMVYDLAK LKKEMSLVLD TVDHRNLDKD VEFFKTTVST SENVAIYMFE KLKSVMSNPS 130 140 VLYKVTIEET PKNIFTYKGC « Hide
Isoform b [UniParc]. Checksum: C5D18E885E24B16B Show »	FASTA	137	15,612
10 20 30 40 50 60 MPIVTMERVD SFSAAHRLHS EKLSDAENKE TFGKCNNSNG HGHNYVWKVK LRGEVDPTSG 70 80 90 100 110 120 MVYDLAKLKK EMSLVLDTVD HRNLDKDVEF FKTTVSTSEN VAIYMFEKLK SVMSNPSVLY 130 KVTIEETPKN IFTYKGC « Hide

References

[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

FO080106 Genomic DNA. Translation: CCD61251.1.
FO080106 Genomic DNA. Translation: CCD61252.1.

PIR

T34033.

RefSeq

NP_001040626.1. NM_001047161.1.
NP_001040627.1. NM_001047162.1.

UniGene

Cel.23560.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2G64	X-ray	1.80	A	1-139	[»]

ProteinModelPortal

O02058.

SMR

O02058. Positions 1-139.

ModBase

Search...

Protein-protein interaction databases

IntAct

O02058. 2 interactions.

MINT

MINT-1061009.

STRING

O02058.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblMetazoa

B0041.6a; B0041.6a; B0041.6.

GeneID

181823.

KEGG

cel:B0041.6.

NMPDR

fig|6239.3.peg.980.

UCSC

B0041.6b. c. elegans.

Organism-specific databases

CTD

181823.

WormBase

B0041.6a; CE34237; WBGene00015010; ptps-1.
B0041.6b; CE39541; WBGene00015010; ptps-1.

Phylogenomic databases

eggNOG

meNOG18734.

GeneTree

EMGT00050000016422.

HOGENOM

HBG727155.

InParanoid

O02058.

OMA

HKNLDKD.

PhylomeDB

O02058.

Gene expression databases

ArrayExpress

O02058.

Family and domain databases

InterPro

IPR007116. 6-carboxy/6-PTP_synth.
IPR007115. 6-PTP_synth-rel.
IPR022470. PTPS_Cys_AS.
[Graphical view]

K01737.

PANTHER

PTHR12589. 6_PTP_synth. 1 hit.

Pfam

PF01242. PTPS. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00039. 6PTHBS. 1 hit.

PROSITE

PS00987. PTPS_1. 1 hit.
PS00988. PTPS_2. False negative.
[Graphical view]

ProtoNet

Search...

Other

NextBio

915462.

Entry information

Entry name

PTPS_CAEEL

Accession

Primary (citable) accession number: O02058
Secondary accession number(s): Q2MGF4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	October 10, 2003
Last modified:	December 14, 2011
This is version 93 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project

Relevant documents

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormPep

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Alternative products

Sequence annotation (Features)

Molecule processing

Sites

Natural variations

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents