Reviewed, UniProtKB/Swiss-Prot O02058 (PTPS_CAEEL)

Names and origin

Protein names

Recommended name:
    Putative 6-pyruvoyl tetrahydrobiopterin synthase
      Short name=PTP synthase
      Short name=PTPS
    EC=4.2.3.12

Gene names

Name:	ptps-1
ORF Names:	B0041.6

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	140 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in the biosynthesis of tetrahydrobiopterin, an essential cofactor of aromatic amino acid hydroxylases. Catalyzes the transformation of 7,8-dihydroneopterin triphosphate into 6-pyruvoyl tetrahydropterin By similarity.
Catalytic activity	7,8-dihydroneopterin 3'-triphosphate = 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	Cofactor biosynthesis; tetrahydrobiopterin biosynthesis; tetrahydrobiopterin from 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)-dihydropteridine triphosphate: step 1/3.
Subunit structure	Homohexamer formed of two homotrimers in a head to head fashion By similarity.
Miscellaneous	The active site is at the interface between 2 subunits. The proton acceptor Cys is on one subunit, and the charge relay system is on the other subunit.
Sequence similarities	Belongs to the PTPS family.

Ontologies

Keywords
Biological process	Tetrahydrobiopterin biosynthesis
Coding sequence diversity	Alternative splicing
Ligand	Metal-binding Zinc
Molecular function	Lyase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	tetrahydrobiopterin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	6-pyruvoyltetrahydropterin synthase activity Inferred from electronic annotation. Source: EC identical protein binding Inferred from physical interaction. Source: IntAct zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		2	EBI-312091,EBI-312091

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform a (identifier: O02058-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: No experimental confirmation available.

Isoform b (identifier: O02058-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-3: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 140

140

Putative 6-pyruvoyl tetrahydrobiopterin synthase

PRO_0000057919

Sites

Active site

38

1

Proton acceptor By similarity

Active site

84

1

Charge relay system By similarity

Active site

129

1

Charge relay system By similarity

Metal binding

19

1

Zinc By similarity

Metal binding

44

1

Zinc By similarity

Metal binding

46

1

Zinc By similarity

Natural variations

Alternative sequence

1 – 3

3

Missing in isoform b.

VSP_019291

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

140

Helix Strand Turn

Details...

Beta strand

6 – 19

14

Helix

28 – 35

8

Helix

36 – 39

4

Beta strand

44 – 57

14

Turn

60 – 62

3

Helix

68 – 80

13

Turn

81 – 84

4

Helix

87 – 90

4

Helix

92 – 95

4

Helix

101 – 115

15

Helix

119 – 121

3

Beta strand

122 – 130

9

Beta strand

133 – 137

5

Sequences

	Sequence		Length	Mass (Da)
	Isoform a [UniParc]. Last modified October 10, 2003. Version 2. Checksum: 7A677CE73A95882C	FASTA	140	16,046
	10 20 30 40 50 60 MFRMPIVTME RVDSFSAAHR LHSEKLSDAE NKETFGKCNN SNGHGHNYVW KVKLRGEVDP 70 80 90 100 110 120 TSGMVYDLAK LKKEMSLVLD TVDHRNLDKD VEFFKTTVST SENVAIYMFE KLKSVMSNPS 130 140 VLYKVTIEET PKNIFTYKGC
	Isoform b. Checksum: C5D18E885E24B16B	FASTA	137	15,612
	10 20 30 40 50 60 MPIVTMERVD SFSAAHRLHS EKLSDAENKE TFGKCNNSNG HGHNYVWKVK LRGEVDPTSG 70 80 90 100 110 120 MVYDLAKLKK EMSLVLDTVD HRNLDKDVEF FKTTVSTSEN VAIYMFEKLK SVMSNPSVLY 130 KVTIEETPKN IFTYKGC

References

[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING. Strain: Bristol N2.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AF000196 Genomic DNA. Translation: AAC24255.2.
AF000196 Genomic DNA. Translation: ABC71844.1.

PIR

T34033.

RefSeq

NP_001040626.1.

UniGene

Cel.23560

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2G64	X-ray	1.80	A	1-139	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

O02058. 1 interaction.

Genome annotation databases

Ensembl

B0041.6. Caenorhabditis elegans. [Contig view]

GeneID

181823.

KEGG

cel:B0041.6.

NMPDR

fig|6239.3.peg.980.

Organism-specific databases

WormBase

WBGene00015010. ptps-1.

WormPep

B0041.6a. CE34237. [WorfDB]
B0041.6b. CE39541. [WorfDB]

Phylogenomic databases

OMA

O02058. AAHRLFN.

Enzyme and pathway databases

BRENDA

4.2.3.12. 672.

Gene expression databases

ArrayExpress

O02058.

Family and domain databases

InterPro

IPR007116. 6-PTP_synth.
IPR007115. 6-PTP_synth-rel.
[Graphical view]

PANTHER

PTHR12589. 6_PTP_synth. 1 hit.

Pfam

PF01242. PTPS. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00039. 6PTHBS. 1 hit.

PROSITE

PS00987. PTPS_1. 1 hit.
PS00988. PTPS_2. False negative.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

915462.

Entry information

Entry name

PTPS_CAEEL

Accession

Primary (citable) accession number: O02058
Secondary accession number(s): Q2MGF4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	October 10, 2003
Last modified:	June 16, 2009
This is version 72 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project