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Protein

Putative 6-pyruvoyl tetrahydrobiopterin synthase

Gene

ptps-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the biosynthesis of tetrahydrobiopterin, an essential cofactor of aromatic amino acid hydroxylases. Catalyzes the transformation of 7,8-dihydroneopterin triphosphate into 6-pyruvoyl tetrahydropterin (By similarity).By similarity

Catalytic activityi

7,8-dihydroneopterin 3'-triphosphate = 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Pathwayi: tetrahydrobiopterin biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes tetrahydrobiopterin from 7,8-dihydroneopterin triphosphate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Putative 6-pyruvoyl tetrahydrobiopterin synthase (ptps-1)
  2. no protein annotated in this organism
  3. no protein annotated in this organism
This subpathway is part of the pathway tetrahydrobiopterin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes tetrahydrobiopterin from 7,8-dihydroneopterin triphosphate, the pathway tetrahydrobiopterin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi19 – 191ZincPROSITE-ProRule annotation
Active sitei38 – 381Proton acceptorPROSITE-ProRule annotation
Metal bindingi44 – 441ZincPROSITE-ProRule annotation
Metal bindingi46 – 461ZincPROSITE-ProRule annotation
Active sitei84 – 841Charge relay systemBy similarity
Active sitei129 – 1291Charge relay systemBy similarity

GO - Molecular functioni

  • 6-pyruvoyltetrahydropterin synthase activity Source: WormBase
  • identical protein binding Source: IntAct
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • tetrahydrobiopterin biosynthetic process Source: WormBase
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Tetrahydrobiopterin biosynthesis

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-CEL-1474151. Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation.
UniPathwayiUPA00849; UER00819.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative 6-pyruvoyl tetrahydrobiopterin synthase (EC:4.2.3.12)
Short name:
PTP synthase
Short name:
PTPS
Gene namesi
Name:ptps-1
ORF Names:B0041.6
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome I

Organism-specific databases

WormBaseiB0041.6a; CE34237; WBGene00015010; ptps-1.
B0041.6b; CE39541; WBGene00015010; ptps-1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 140140Putative 6-pyruvoyl tetrahydrobiopterin synthasePRO_0000057919Add
BLAST

Proteomic databases

EPDiO02058.
PaxDbiO02058.

Interactioni

Subunit structurei

Homohexamer formed of two homotrimers in a head to head fashion.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
itself4EBI-312091,EBI-312091

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi46692. 1 interaction.
IntActiO02058. 1 interaction.
MINTiMINT-1061009.
STRINGi6239.B0041.6a.

Structurei

Secondary structure

1
140
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi6 – 1914Combined sources
Helixi28 – 358Combined sources
Helixi36 – 394Combined sources
Beta strandi44 – 5714Combined sources
Turni60 – 623Combined sources
Helixi68 – 8013Combined sources
Turni81 – 844Combined sources
Helixi87 – 904Combined sources
Helixi92 – 954Combined sources
Helixi101 – 11515Combined sources
Helixi119 – 1213Combined sources
Beta strandi122 – 1309Combined sources
Beta strandi133 – 1375Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2G64X-ray1.80A1-140[»]
ProteinModelPortaliO02058.
SMRiO02058. Positions 1-139.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO02058.

Family & Domainsi

Sequence similaritiesi

Belongs to the PTPS family.Curated

Phylogenomic databases

eggNOGiKOG4105. Eukaryota.
COG0720. LUCA.
GeneTreeiENSGT00390000002752.
HOGENOMiHOG000225069.
InParanoidiO02058.
KOiK01737.
OMAiHGHNYVW.
OrthoDBiEOG7NSB3V.
PhylomeDBiO02058.

Family and domain databases

InterProiIPR007115. 6-PTP_synth/QueD.
IPR022470. PTPS_Cys_AS.
[Graphical view]
PANTHERiPTHR12589. PTHR12589. 1 hit.
PfamiPF01242. PTPS. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00039. 6PTHBS. 1 hit.
PROSITEiPS00987. PTPS_1. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform a (identifier: O02058-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MFRMPIVTME RVDSFSAAHR LHSEKLSDAE NKETFGKCNN SNGHGHNYVW
60 70 80 90 100
KVKLRGEVDP TSGMVYDLAK LKKEMSLVLD TVDHRNLDKD VEFFKTTVST
110 120 130 140
SENVAIYMFE KLKSVMSNPS VLYKVTIEET PKNIFTYKGC
Note: No experimental confirmation available.
Length:140
Mass (Da):16,046
Last modified:October 10, 2003 - v2
Checksum:i7A677CE73A95882C
GO
Isoform b (identifier: O02058-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-3: Missing.

Note: No experimental confirmation available.
Show »
Length:137
Mass (Da):15,612
Checksum:iC5D18E885E24B16B
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 33Missing in isoform b. CuratedVSP_019291

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO080106 Genomic DNA. Translation: CCD61251.1.
FO080106 Genomic DNA. Translation: CCD61252.1.
PIRiT34033.
RefSeqiNP_001040626.1. NM_001047161.1. [O02058-1]
NP_001040627.1. NM_001047162.2. [O02058-2]
UniGeneiCel.23560.

Genome annotation databases

EnsemblMetazoaiB0041.6a; B0041.6a; WBGene00015010. [O02058-1]
GeneIDi181823.
KEGGicel:CELE_B0041.6.
UCSCiB0041.6b. c. elegans. [O02058-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO080106 Genomic DNA. Translation: CCD61251.1.
FO080106 Genomic DNA. Translation: CCD61252.1.
PIRiT34033.
RefSeqiNP_001040626.1. NM_001047161.1. [O02058-1]
NP_001040627.1. NM_001047162.2. [O02058-2]
UniGeneiCel.23560.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2G64X-ray1.80A1-140[»]
ProteinModelPortaliO02058.
SMRiO02058. Positions 1-139.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi46692. 1 interaction.
IntActiO02058. 1 interaction.
MINTiMINT-1061009.
STRINGi6239.B0041.6a.

Proteomic databases

EPDiO02058.
PaxDbiO02058.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiB0041.6a; B0041.6a; WBGene00015010. [O02058-1]
GeneIDi181823.
KEGGicel:CELE_B0041.6.
UCSCiB0041.6b. c. elegans. [O02058-1]

Organism-specific databases

CTDi181823.
WormBaseiB0041.6a; CE34237; WBGene00015010; ptps-1.
B0041.6b; CE39541; WBGene00015010; ptps-1.

Phylogenomic databases

eggNOGiKOG4105. Eukaryota.
COG0720. LUCA.
GeneTreeiENSGT00390000002752.
HOGENOMiHOG000225069.
InParanoidiO02058.
KOiK01737.
OMAiHGHNYVW.
OrthoDBiEOG7NSB3V.
PhylomeDBiO02058.

Enzyme and pathway databases

UniPathwayiUPA00849; UER00819.
ReactomeiR-CEL-1474151. Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation.

Miscellaneous databases

EvolutionaryTraceiO02058.
PROiO02058.

Family and domain databases

InterProiIPR007115. 6-PTP_synth/QueD.
IPR022470. PTPS_Cys_AS.
[Graphical view]
PANTHERiPTHR12589. PTHR12589. 1 hit.
PfamiPF01242. PTPS. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00039. 6PTHBS. 1 hit.
PROSITEiPS00987. PTPS_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
    Strain: Bristol N2.

Entry informationi

Entry nameiPTPS_CAEEL
AccessioniPrimary (citable) accession number: O02058
Secondary accession number(s): Q2MGF4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: October 10, 2003
Last modified: June 8, 2016
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Miscellaneous

The active site is at the interface between 2 subunits. The proton acceptor Cys is on one subunit, and the charge relay system is on the other subunit.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.