Skip Header

 
Contribute Send feedback
Read comments (0) or add your own

Reviewed, UniProtKB/Swiss-Prot O02058 (PTPS_CAEEL)

Last modified June 16, 2009. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Putative 6-pyruvoyl tetrahydrobiopterin synthase
      Short name=PTP synthase
      Short name=PTPS
    EC=4.2.3.12
Gene names
Name: ptps-1
ORF Names: B0041.6
OrganismCaenorhabditis elegans [Complete proteome]
Taxonomic identifier6239 [NCBI]
Taxonomic lineageEukaryotaMetazoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis

Protein attributes

Sequence length140 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Involved in the biosynthesis of tetrahydrobiopterin, an essential cofactor of aromatic amino acid hydroxylases. Catalyzes the transformation of 7,8-dihydroneopterin triphosphate into 6-pyruvoyl tetrahydropterin By similarity.

Catalytic activity

7,8-dihydroneopterin 3'-triphosphate = 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate.

Cofactor

Binds 1 zinc ion per subunit By similarity.

Pathway

Cofactor biosynthesis; tetrahydrobiopterin biosynthesis; tetrahydrobiopterin from 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)-dihydropteridine triphosphate: step 1/3.

Subunit structure

Homohexamer formed of two homotrimers in a head to head fashion By similarity.

Miscellaneous

The active site is at the interface between 2 subunits. The proton acceptor Cys is on one subunit, and the charge relay system is on the other subunit.

Sequence similarities

Belongs to the PTPS family.

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

itself2EBI-312091,EBI-312091

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform a (identifier: O02058-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: No experimental confirmation available.
Isoform b (identifier: O02058-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-3: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 140140Putative 6-pyruvoyl tetrahydrobiopterin synthase
PRO_0000057919

Sites

Active site381Proton acceptor By similarity
Active site841Charge relay system By similarity
Active site1291Charge relay system By similarity
Metal binding191Zinc By similarity
Metal binding441Zinc By similarity
Metal binding461Zinc By similarity

Natural variations

Alternative sequence1 – 33Missing in isoform b.
VSP_019291

Secondary structure

........................ 140
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform a [UniParc].

Last modified October 10, 2003. Version 2.
Checksum: 7A677CE73A95882C

FASTA14016,046
        10         20         30         40         50         60 
MFRMPIVTME RVDSFSAAHR LHSEKLSDAE NKETFGKCNN SNGHGHNYVW KVKLRGEVDP 

        70         80         90        100        110        120 
TSGMVYDLAK LKKEMSLVLD TVDHRNLDKD VEFFKTTVST SENVAIYMFE KLKSVMSNPS 

       130        140 
VLYKVTIEET PKNIFTYKGC 

« Hide

Isoform b.

Checksum: C5D18E885E24B16B
Show »

FASTA13715,612

References

[1]"Genome sequence of the nematode C. elegans: a platform for investigating biology."
The C. elegans sequencing consortium
Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
Strain: Bristol N2.

Cross-references

Sequence databases

AF000196 Genomic DNA. Translation: AAC24255.2.
AF000196 Genomic DNA. Translation: ABC71844.1.
PIRT34033.
RefSeqNP_001040626.1.
UniGeneCel.23560

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
2G64X-ray1.80A1-139[»]
ModBaseSearch...

Protein-protein interaction databases

IntActO02058. 1 interaction.

Genome annotation databases

EnsemblB0041.6. Caenorhabditis elegans. [Contig view]
GeneID181823.
KEGGcel:B0041.6.
NMPDRfig|6239.3.peg.980.

Organism-specific databases

WormBaseWBGene00015010. ptps-1.
WormPepB0041.6a. CE34237. [WorfDB]
B0041.6b. CE39541. [WorfDB]

Phylogenomic databases

OMAO02058. AAHRLFN.

Enzyme and pathway databases

BRENDA4.2.3.12. 672.

Gene expression databases

ArrayExpressO02058.

Family and domain databases

InterProIPR007116. 6-PTP_synth.
IPR007115. 6-PTP_synth-rel.
[Graphical view]
PANTHERPTHR12589. 6_PTP_synth. 1 hit.
PfamPF01242. PTPS. 1 hit.
[Graphical view]
TIGRFAMsTIGR00039. 6PTHBS. 1 hit.
PROSITEPS00987. PTPS_1. 1 hit.
PS00988. PTPS_2. False negative.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio915462.

Entry information

Entry namePTPS_CAEEL
AccessionPrimary (citable) accession number: O02058
Secondary accession number(s): Q2MGF4
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: October 10, 2003
Last modified: June 16, 2009
This is version 72 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectCaenorhabditis annotation project

Relevant documents

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormPep

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents