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Reviewed, UniProtKB/Swiss-Prot O01991 (EF2K_CAEEL)

Last modified November 3, 2009. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Elongation factor 2 kinase
    EC=2.7.11.20
Alternative name(s):
    eEF-2 kinase
      Short name=eEF-2K
    Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase
Gene names
Name: efk-1
ORF Names: F42A10.4
OrganismCaenorhabditis elegans [Complete proteome]
Taxonomic identifier6239 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

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Protein attributes

Sequence length768 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at transcript level.

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General annotation (Comments)

Function

Phosphorylates 2 adjacent threonine residues, 'Thr-57' and 'Thr-59', in the N-terminus of eukaryotic elongation factor-2. Binds calmodulin By similarity.

Catalytic activity

ATP + [elongation factor 2] = ADP + [elongation factor 2] phosphate.

Enzyme regulation

Undergoes calcium/calmodulin-dependent intramolecular autophosphorylation, and this results in it becoming partially calcium/calmodulin-independent By similarity.

Subunit structure

Monomer or homodimer Potential.

Sequence similarities

Belongs to the protein kinase superfamily. Alpha-type protein kinase family.

Contains 1 alpha-type protein kinase domain.

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Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform a (identifier: O01991-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform b (identifier: O01991-2)

The sequence of this isoform differs from the canonical sequence as follows:
     625-632: Missing.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 768768Elongation factor 2 kinase
PRO_0000086935

Regions

Domain102 – 309208Alpha-type protein kinase
Nucleotide binding279 – 2846ATP Potential
Compositional bias690 – 6934Poly-Glu

Natural variations

Alternative sequence625 – 6328Missing in isoform b.
VSP_004231

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Sequences

Sequence LengthMass (Da)Tools
Isoform a [UniParc].

Last modified July 1, 1997. Version 1.
Checksum: 9333F3B13F0C14C5

FASTA76887,825
        10         20         30         40         50         60 
MTIDTTNESD NSPTNSPGLE ASARTFSLNA SKMVRITDDY ADEVFIEQND VVIEKPRMDP 

        70         80         90        100        110        120 
LHVRKLMETW RKAARRARTN YIDPWDEFNI HEYPVQRAKR YRYSAIRKQW TEDIVDVRLH 

       130        140        150        160        170        180 
PDSFARGAMR ECYRLKKCSK HGTSQDWSSN YVAKRYICQV DRRVLFDDVR LQMDAKLWAE 

       190        200        210        220        230        240 
EYNRYNPPKK IDIVQMCVIE MIDVKGSPLY HLEHFIEGKY IKYNSNSGFV SNAARLTPQA 

       250        260        270        280        290        300 
FSHFTFERSG HQMMVVDIQG VGDLYTDPQI HTVVGTDYGD GNLGTRGMAL FFHSHRCNDI 

       310        320        330        340        350        360 
CETMDLSNFE LSPPEIEATE VAMEVAAKQK KSCIVPPTVF EARRNRISSE CVHVEHGISM 

       370        380        390        400        410        420 
DQLRKRKTLN QSSTDLSAKS HNEDCVCPEC IPVVEQLCEP CSEDEEDEEE DYPRSEKSGN 

       430        440        450        460        470        480 
SQKSRRSRMS ISTRSSGDES ASRPRKCGFV DLNSLRQRHD SFRSSVGTYS MNSSRQTRDT 

       490        500        510        520        530        540 
EKDEFWKVLR KQSVPANILS LQLQQMAANL ENDEDVPQVT GHQFSVLGQI HIDLSRYHEL 

       550        560        570        580        590        600 
GRFVEVDSEH KEMLEGSEND ARVPIKYDKQ SAIFHLDIAR KCGILEAVLT SAHIVLGLPH 

       610        620        630        640        650        660 
ELLKEVTVDD LFPNGFGEQE NGIRADKGQK PCDLEEFGSD LMEIAAEMGD KGAMLYMAHA 

       670        680        690        700        710        720 
YETGQHLGPN RRTDYKKSID WYQRVVGFQE EEELDSDCGK TTFSSFAPLT RHEILAKMAE 

       730        740        750        760 
MYKEGGYGLN QDFERAYGLF NEAAEAAMEA MNGKLANKYY EKAEMCGE

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Isoform b.

Checksum: 0359C8F838069B65
Show »

FASTA76086,997

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References

« Hide 'large scale' references
[1]"Identification of a new class of protein kinases represented by eukaryotic elongation factor-2 kinase."
Ryazanov A.G., Ward M.D., Mendola C.E., Pavur K.S., Dorovkov M.V., Wiedmann M., Erdjument-Bromage H., Tempst P., Parmer T.G., Prostko C.R., Germino F.J., Hait W.N.
Proc. Natl. Acad. Sci. U.S.A. 94:4884-4889(1997) [PubMed: 9144159] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A AND B).
Strain: Bristol N2.
[2]"Genome sequence of the nematode C. elegans: a platform for investigating biology."
The C. elegans sequencing consortium
Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
Strain: Bristol N2.
+Additional computationally mapped references.

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Cross-references

Sequence databases

U93846 mRNA. Translation: AAB58268.1.
U93847 mRNA. Translation: AAB58269.1.
U10414 Genomic DNA. Translation: AAN63383.1.
U10414 Genomic DNA. Translation: AAN63384.1.
RefSeqNP_001022572.1.
NP_001022573.1.
UniGeneCel.5654

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

IntActO01991. 2 interactions.

Proteomic databases

PRIDEO01991.

Genome annotation databases

EnsemblF42A10.4a.1; F42A10.4a.1; F42A10.4; Caenorhabditis elegans. [Genome view]
F42A10.4a.2; F42A10.4a.2; F42A10.4; Caenorhabditis elegans. [Genome view]
F42A10.4b; F42A10.4b; F42A10.4; Caenorhabditis elegans. [Genome view]
GeneID175871.
KEGGcel:F42A10.4.
UCSCF42A10.4a.1. c. elegans.

Organism-specific databases

CTD175871.
WormBaseWBGene00001160. efk-1.
WormPepF42A10.4a. CE32415. [WorfDB]
F42A10.4b. CE32416. [WorfDB]

Phylogenomic databases

OMACNDICET.

Enzyme and pathway databases

BRENDA2.7.11.20. 672.

Gene expression databases

ArrayExpressO01991.

Family and domain databases

InterProIPR017400. Elongation_factor_2_kinase.
IPR004166. MHCK_EF2_kinase.
IPR006597. Sel1-like.
[Graphical view]
PfamPF02816. Alpha_kinase. 1 hit.
PF08238. Sel1. 2 hits.
[Graphical view]
PIRSFPIRSF038139. Elongation_factor_2_kinase. 1 hit.
SMARTSM00811. Alpha_kinase. 1 hit.
SM00671. SEL1. 2 hits.
[Graphical view]
PROSITEPS51158. ALPHA_KINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio890082.

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Entry information

Entry nameEF2K_CAEEL
AccessionPrimary (citable) accession number: O01991
Secondary accession number(s): O01992, Q20309, Q8MYP9
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 1, 1997
Last modified: November 3, 2009
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectCaenorhabditis annotation project

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Relevant documents

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormPep

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents