Reviewed, UniProtKB/Swiss-Prot O01949 (ALL3_AEDAE)

Names and origin

Protein names

Recommended name:
    30 kDa salivary gland allergen Aed a 3
Alternative name(s):
    Allergen=Aed a 3

Gene names

ORF Names:

AAEL010235

Organism

Aedes aegypti (Yellowfever mosquito) (Culex aegypti) [Complete proteome]

Taxonomic identifier

7159 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Nematocera › Culicoidea › Culicidae › Culicinae › Culicini › Aedes › Stegomyia

Protein attributes

Sequence length	273 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Subcellular location	Secreted Potential.
Tissue specificity	Adult female salivary gland.
Allergenic properties	Causes an allergic reaction in human.

Ontologies

Keywords
Cellular component	Secreted
Coding sequence diversity	Alternative splicing
Disease	Allergen
Domain	Signal
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 2 (identifier: O01949-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 1 (identifier: O01949-2)

The sequence of this isoform differs from the canonical sequence as follows:
     35-44: Missing.
     85-94: Missing.

Isoform 3 (identifier: O01949-3)

The sequence of this isoform differs from the canonical sequence as follows:
     35-44: Missing.
     85-89: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Chain	20 – 273	254	30 kDa salivary gland allergen Aed a 3		PRO_0000020676
Regions
	Compositional bias	24 – 146	123	Glu-rich
Natural variations
	Alternative sequence	35 – 44	10	Missing in isoform 1 and isoform 3.		VSP_034685
	Alternative sequence	85 – 94	10	Missing in isoform 1.		VSP_034686
	Alternative sequence	85 – 89	5	Missing in isoform 3.		VSP_034687
Experimental info
	Sequence conflict	69	1	K → E in AAB58417. Ref.1
	Sequence conflict	69	1	K → E in ABF18125. Ref.2
	Sequence conflict	143	1	D → A in AAB58417. Ref.1
	Sequence conflict	143	1	D → A in ABF18125. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 2 [UniParc]. Last modified July 22, 2008. Version 2. Checksum: C827EFA0FE76E721	FASTA	273	29,155
	10 20 30 40 50 60 MKPLVKLFLL FCLVGIVLSR PMPEDEEPVA EGGDDDASGE SEGEEETTDD AGGDGGEEEN 70 80 90 100 110 120 EGEEHAGDKD AGGEDTGKEE NTGHDDAGEE DAGEEDAGEE DAGEEDAGEE DAEKEEGEKE 130 140 150 160 170 180 DAGDDAGSDD GEEDSTGGDE GEDNAEDSKG SEKNDPADTY RQVVALLDKD TKVDHIQSEY 190 200 210 220 230 240 LRSALNNDLQ SEVRVPVVEA IGRIGDYSKI QGCFKSMGKD VKKVISEEEK KFKSCMSKKK 250 260 270 SEYQCSEDSF AAAKSKLSPI TSKIKSCVSS KGR
	Isoform 1. Checksum: 6D2F6069E547D01C	FASTA	253	27,189
	10 20 30 40 50 60 MKPLVKLFLL FCLVGIVLSR PMPEDEEPVA EGGDEETTDD AGGDGGEEEN EGEEHAGDKD 70 80 90 100 110 120 AGGEDTGKEE NTGHEDAGEE DAGEEDAGEE DAEKEEGEKE DAGDDAGSDD GEEDSTGGDE 130 140 150 160 170 180 GEDNAEDSKG SEKNDPADTY RQVVALLDKD TKVDHIQSEY LRSALNNDLQ SEVRVPVVEA 190 200 210 220 230 240 IGRIGDYSKI QGCFKSMGKD VKKVISEEEK KFKSCMSKKK SEYQCSEDSF AAAKSKLSPI 250 TSKIKSCVSS KGR
	Isoform 3. Checksum: D0D8AF65DCFE1581	FASTA	258	27,691
	10 20 30 40 50 60 MKPLVKLFLL FCLVGIVLSR PMPEDEEPVA EGGDEETTDD AGGDGGEEEN EGEEHAGDKD 70 80 90 100 110 120 AGGEDTGKEE NTGHEDAGEE DAGEEDAGEE DAGEEDAEKE EGEKEDAGDD AGSDDGEEDS 130 140 150 160 170 180 TGGDEGEDNA EDSKGSEKND PADTYRQVVA LLDKDTKVDH IQSEYLRSAL NNDLQSEVRV 190 200 210 220 230 240 PVVEAIGRIG DYSKIQGCFK SMGKDVKKVI SEEEKKFKSC MSKKKSEYQC SEDSFAAAKS 250 KLSPITSKIK SCVSSKGR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and characterization of the gene encoding a 30 kDa salivary allergen of mosquito Aedes aegypti." Xu W., Simons F.E.R., Peng Z. Submitted (MAY-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Strain: Rockefeller. Tissue: Salivary gland.
[2]	"An annotated catalogue of salivary gland transcripts in the adult female mosquito, Aedes aegypti." Ribeiro J.M.C., Arca B., Lombardo F., Calvo E., Phan V.M., Chandra P.K., Wikel S.K. BMC Genomics 8:6-6(2007) [PubMed: 17204158] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3). Strain: Black-eyed Liverpool. Tissue: Salivary gland.
[3]	"Genome sequence of Aedes aegypti, a major arbovirus vector." Nene V., Wortman J.R., Lawson D., Haas B.J., Kodira C.D., Tu Z.J., Loftus B.J., Xi Z., Megy K., Grabherr M., Ren Q., Zdobnov E.M., Lobo N.F., Campbell K.S., Brown S.E., Bonaldo M.F., Zhu J., Sinkins S.P. , Hogenkamp D.G., Amedeo P., Arensburger P., Atkinson P.W., Bidwell S.L., Biedler J., Birney E., Bruggner R.V., Costas J., Coy M.R., Crabtree J., Crawford M., DeBruyn B., DeCaprio D., Eiglmeier K., Eisenstadt E., El-Dorry H., Gelbart W.M., Gomes S.L., Hammond M., Hannick L.I., Hogan J.R., Holmes M.H., Jaffe D., Johnston S.J., Kennedy R.C., Koo H., Kravitz S., Kriventseva E.V., Kulp D., Labutti K., Lee E., Li S., Lovin D.D., Mao C., Mauceli E., Menck C.F., Miller J.R., Montgomery P., Mori A., Nascimento A.L., Naveira H.F., Nusbaum C., O'Leary S.B., Orvis J., Pertea M., Quesneville H., Reidenbach K.R., Rogers Y.-H.C., Roth C.W., Schneider J.R., Schatz M., Shumway M., Stanke M., Stinson E.O., Tubio J.M.C., Vanzee J.P., Verjovski-Almeida S., Werner D., White O.R., Wyder S., Zeng Q., Zhao Q., Zhao Y., Hill C.A., Raikhel A.S., Soares M.B., Knudson D.L., Lee N.H., Galagan J., Salzberg S.L., Paulsen I.T., Dimopoulos G., Collins F.H., Bruce B., Fraser-Liggett C.M., Severson D.W. Science 316:1718-1723(2007) [PubMed: 17510324] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Liverpool.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF001927 mRNA. Translation: AAB58417.1. DQ440089 mRNA. Translation: ABF18122.1. DQ440092 mRNA. Translation: ABF18125.1. CH477649 Genomic DNA. Translation: EAT37812.1.
RefSeq	XP_001660691.1.
UniGene	Aae.1631 Aae.23882
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5580040.
KEGG	aag:AaeL_AAEL010235.
VectorBase	AAEL010235. Aedes aegypti.
Phylogenomic databases
OrthoDB	EOG9X97QP
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

ALL3_AEDAE

Accession

Primary (citable) accession number: O01949
Secondary accession number(s): Q1HRK2, Q1HRK5

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2001
Last sequence update:	July 22, 2008
Last modified:	November 24, 2009
This is version 47 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)