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Protein

ATP-dependent RNA helicase glh-3

Gene

glh-3

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable ATP-binding RNA helicase.

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri202 – 21918CCHC-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri222 – 23918CCHC-type 2PROSITE-ProRule annotationAdd
BLAST
Nucleotide bindingi342 – 3498ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ATP-dependent RNA helicase activity Source: GO_Central
  • DEAD/H-box RNA helicase binding Source: WormBase
  • JUN kinase binding Source: WormBase
  • protein self-association Source: WormBase
  • RNA binding Source: UniProtKB-KW
  • zinc ion binding Source: InterPro

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, RNA-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent RNA helicase glh-3 (EC:3.6.4.13)
Alternative name(s):
Germline helicase 3
Gene namesi
Name:glh-3
ORF Names:B0414.6
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome I

Organism-specific databases

WormBaseiB0414.6; CE07736; WBGene00001600; glh-3.

Subcellular locationi

GO - Cellular componenti

  • P granule Source: WormBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 720720ATP-dependent RNA helicase glh-3PRO_0000055091Add
BLAST

Proteomic databases

EPDiO01836.
PaxDbiO01836.

PTM databases

iPTMnetiO01836.

Expressioni

Developmental stagei

During germline proliferation.

Interactioni

Subunit structurei

Interacts with csn-5. Interacts (via C-terminus) with kgb-1. Interacts with zyx-1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
csn-5P910012EBI-1571750,EBI-313007
glh-1P346892EBI-1571750,EBI-1571791
kgb-1O444082EBI-1571750,EBI-319489
zyx-1Q9U3F42EBI-1571750,EBI-322208

GO - Molecular functioni

  • DEAD/H-box RNA helicase binding Source: WormBase
  • JUN kinase binding Source: WormBase
  • protein self-association Source: WormBase

Protein-protein interaction databases

IntActiO01836. 4 interactions.
STRINGi6239.B0414.6.2.

Structurei

3D structure databases

ProteinModelPortaliO01836.
SMRiO01836. Positions 203-241, 253-698.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini329 – 513185Helicase ATP-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini549 – 698150Helicase C-terminalPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi298 – 32629Q motifAdd
BLAST
Motifi456 – 4594DEAD box

Sequence similaritiesi

Contains 2 CCHC-type zinc fingers.PROSITE-ProRule annotation
Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri202 – 21918CCHC-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri222 – 23918CCHC-type 2PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG0335. Eukaryota.
ENOG410XNTI. LUCA.
GeneTreeiENSGT00770000120531.
InParanoidiO01836.
PhylomeDBiO01836.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
4.10.60.10. 1 hit.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
IPR001878. Znf_CCHC.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
PF00098. zf-CCHC. 2 hits.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SM00343. ZnF_C2HC. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF57756. SSF57756. 1 hit.
PROSITEiPS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
PS50158. ZF_CCHC. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O01836-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDKSPTKTSI RTKFARHQPI SDVDTTEQSS SCIKKDDRGL SSFGVQSSVF
60 70 80 90 100
SRRSCRMSEL EAKPTIISED QRIAVRSEIG GSFSGFDDKV DNVFHSNNNL
110 120 130 140 150
HGSPSTTELE CPGIMNPRFL VGRSLNSRSR AVTRGSKRTS NVKENEGSIH
160 170 180 190 200
RSDDQVSTEN CSAKDEERDR DSGGVSSYGN KRSDEFCGTS PILEAKGFGI
210 220 230 240 250
SNTCFNCKKY GHRATECSAP QRECANCGDP NHRANECASW SKNGVQEPTK
260 270 280 290 300
VTYVPVVDKM EEVFSMLKIN AGDFFDKFFD ASVQLVSRGQ PVTIQPCKSF
310 320 330 340 350
SDSDIPQSMR RNVERAGYTR TTPIQQYTLP LVADGKDILA CAQTGSGKTA
360 370 380 390 400
AFLLPIMSRL ILEKDLNYGA EGGCYPRCII LTPTRELADQ IYNEGRKFSY
410 420 430 440 450
QSVMEIKPVY GGINVGYNKS QIMKGCTIIV GTIGRVKHFC EDGAIKLDKC
460 470 480 490 500
RYLVLDEADR MIDSMGFGPE IEQIINYKNM PKNDKRQTMM FSATFPSSVQ
510 520 530 540 550
EAARKLLRED YTMITIDKIG AANKCVIQEF ELCDRTSKVD KLLKLLGIDI
560 570 580 590 600
DTYTTEKNSD VFVKKTIVFV AQQKMADTLA SIMSAAQVPA ITIHGAREQK
610 620 630 640 650
ERSAALKLFR SGAKPVLIAT AVVERGLDIK GVDHVINYDM PNNIDDYIHR
660 670 680 690 700
IGRTGRVGNS GRATSFISLA DDVQILPQLV RTLADAEQVV PSWMKEAAGG
710 720
TSNPNKFEKS IDTEEPEEAW
Length:720
Mass (Da):79,728
Last modified:July 1, 1997 - v1
Checksum:iA91F49FE2B79DEAA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF079509 mRNA. Translation: AAC28388.1.
FO080197 Genomic DNA. Translation: CCD61900.1.
PIRiT15231.
RefSeqiNP_491681.1. NM_059280.4.
UniGeneiCel.19392.

Genome annotation databases

EnsemblMetazoaiB0414.6; B0414.6; WBGene00001600.
GeneIDi172245.
KEGGicel:CELE_B0414.6.
UCSCiB0414.6. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF079509 mRNA. Translation: AAC28388.1.
FO080197 Genomic DNA. Translation: CCD61900.1.
PIRiT15231.
RefSeqiNP_491681.1. NM_059280.4.
UniGeneiCel.19392.

3D structure databases

ProteinModelPortaliO01836.
SMRiO01836. Positions 203-241, 253-698.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO01836. 4 interactions.
STRINGi6239.B0414.6.2.

PTM databases

iPTMnetiO01836.

Proteomic databases

EPDiO01836.
PaxDbiO01836.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiB0414.6; B0414.6; WBGene00001600.
GeneIDi172245.
KEGGicel:CELE_B0414.6.
UCSCiB0414.6. c. elegans.

Organism-specific databases

CTDi172245.
WormBaseiB0414.6; CE07736; WBGene00001600; glh-3.

Phylogenomic databases

eggNOGiKOG0335. Eukaryota.
ENOG410XNTI. LUCA.
GeneTreeiENSGT00770000120531.
InParanoidiO01836.
PhylomeDBiO01836.

Miscellaneous databases

PROiO01836.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
4.10.60.10. 1 hit.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
IPR001878. Znf_CCHC.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
PF00098. zf-CCHC. 2 hits.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SM00343. ZnF_C2HC. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF57756. SSF57756. 1 hit.
PROSITEiPS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
PS50158. ZF_CCHC. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Combinatorial RNA interference indicates GLH-4 can compensate for GLH-1; these two P granule components are critical for fertility in C. elegans."
    Kuznicki K.A., Smith P.A., Leung-Chiu W.M., Estevez A.O., Scott H.C., Bennett K.L.
    Development 127:2907-2916(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Bristol N2.
  2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.
  3. "The GLH proteins, Caenorhabditis elegans P granule components, associate with CSN-5 and KGB-1, proteins necessary for fertility, and with ZYX-1, a predicted cytoskeletal protein."
    Smith P., Leung-Chiu W.-M., Montgomery R., Orsborn A., Kuznicki K., Gressman-Coberly E., Mutapcic L., Bennett K.
    Dev. Biol. 251:333-347(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CSN-5; KGB-1 AND ZYX-1.

Entry informationi

Entry nameiGLH3_CAEEL
AccessioniPrimary (citable) accession number: O01836
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: July 1, 1997
Last modified: July 6, 2016
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.