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Protein

Ras-related protein rab-11.1

Gene

rab-11.1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes (PubMed:21320697, PubMed:24843160). Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion (PubMed:21320697, PubMed:24843160). Involved in regulating the meiotic maturation of oocytes (PubMed:18472420). Plays a role in egg shell formation, regulating exocytosis of chrondroitin proteoglycans following fertilization (PubMed:18765566, PubMed:26506309). Controls cortical granule localization and targets them to the plasma membrane for exocytosis (PubMed:18765566). Acts as a major regulator of membrane delivery during cytokinesis (PubMed:18765566, PubMed:20116245, PubMed:26506309). Regulates the cytoskeleton by facilitating astral microtubule elongation and organization during metaphase to ensure proper spindle alignment and polarity in the first embryonic cell division (PubMed:18385514). Maintains normal endoplasmic reticulum morphology during metaphase (PubMed:18385514). Involved in vesicle formation and plasma membrane repair following exposure to pore forming toxins (PubMed:21320697). Regulates endocytic recycling (PubMed:24843160). May play a role in yolk receptor endocytosis in growing oocytes (PubMed:18354496, PubMed:26506309). Plays a role in the shedding of pathogen spores from intestinal cells via its involvement in spore fusion and endocytic trafficking (PubMed:24843160).8 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi18 – 26GTPBy similarity9
Nucleotide bindingi66 – 70GTPBy similarity5
Nucleotide bindingi124 – 127GTPBy similarity4
Nucleotide bindingi154 – 156GTPBy similarity3

GO - Molecular functioni

  • GTPase activity Source: WormBase
  • GTP binding Source: WormBase

GO - Biological processi

  • cortical granule exocytosis Source: WormBase
  • eggshell formation Source: WormBase
  • embryo development ending in birth or egg hatching Source: WormBase
  • positive regulation of cytokinesis Source: WormBase
  • protein transport Source: UniProtKB-KW
  • receptor-mediated endocytosis Source: WormBase
  • small GTPase mediated signal transduction Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-CEL-5620912. Anchoring of the basal body to the plasma membrane.
R-CEL-5620916. VxPx cargo-targeting to cilium.

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related protein rab-11.1By similarity
Alternative name(s):
Rab GTPase rab-11.1Curated
Gene namesi
Name:rab-11.1Imported
ORF Names:F53G12.1Imported
OrganismiCaenorhabditis elegansImported
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome I

Organism-specific databases

WormBaseiF53G12.1; CE11006; WBGene00004274; rab-11.1.

Subcellular locationi

GO - Cellular componenti

  • apical plasma membrane Source: UniProtKB-SubCell
  • cortical granule Source: WormBase
  • cytosol Source: UniProtKB-SubCell
  • Golgi membrane Source: UniProtKB-SubCell
  • microtubule organizing center Source: UniProtKB-SubCell
  • recycling endosome Source: WormBase
  • recycling endosome membrane Source: UniProtKB-SubCell
  • spindle Source: UniProtKB-SubCell
  • transport vesicle Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Endosome, Golgi apparatus, Membrane

Pathology & Biotechi

Disruption phenotypei

Zygotic lethal (PubMed:18765566). RNAi-mediated knockdown results in a reduced meiotic maturation rate of oocytes (PubMed:18472420). Embryos are multinucleated and osmotically sensitive with permeable and therefore defective eggshells (PubMed:18765566, PubMed:18385514). During the first cell cycle, 52% of embryos fail to extrude polar bodies, 83% of embryos display no or minimal pseudocleavage and the centrosomal-nuclear complex does not migrate to the center of embryos (PubMed:18385514). Animals display mitotic spindle alignment defects whereby in 73.9% of embryos, the P0 spindle does not rotate to the anterior-posterior axis of the embryo during anaphase, but during the metaphase to anaphase transition, the movements of the spindle are more abrupt with the spindle migrating to the posterior pole and then rebounding to the anterior-posterior axis (PubMed:18385514). In 17.4% of embryos the centrosomes are unstable and the spindle is displaced further towards the posterior pole (PubMed:18385514). Impaired localization of proteins important for establishing cell polarity such as par-2, par-3, gpr-1 and gpr-2 (PubMed:18385514). Altered microtubule organization and dynamics during metaphase including a greater distance between the growing microtubule plus ends and the posterior cortex, impeded microtubule growth with fewer growing astral microtubules reaching the cortex, and a reduced microtubule nucleation rate (PubMed:18385514). Slight defect in endoplasmic reticulum morphology whereby the large endoplasmic reticulum aggregates that normally form during metaphase and persist during anaphase, form, but do not persist during anaphase and disperse (PubMed:18385514). Mild endocytosis defect with an accumulation of the yolk protein vitellogenin in the pseudocoelom (PubMed:18354496). Impaired cortical granule (secretory vesicle) localization with granules abnormally clustered around the nuclear envelope of proximal oocytes, irregulary dispersed in the cytoplasm of oocytes and retained in the embryo following entry into the uterus (PubMed:18765566). Distrupted fusion of spores, containing intracellular pathogen N.parisii, with the apical cell membrane and thus disrupted clearance from intestinal cells (PubMed:24843160). RNAi-mediated knockdown in RNAi-sensitive mutants exposed to bacterial pore-forming toxin Cry5B results in intoxification and impaired plasma membrane repair (PubMed:21320697). RNAi-mediated knockdown in the intestine results in reduced survival upon exposure to Cry5B (PubMed:21320697).6 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004356821 – 211Ras-related protein rab-11.1CuratedAdd BLAST211

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi208S-geranylgeranyl cysteineBy similarity1
Lipidationi209S-geranylgeranyl cysteineBy similarity1

Keywords - PTMi

Lipoprotein, Prenylation

Proteomic databases

EPDiO01803.
PaxDbiI7EVK5.
O01803.
PeptideAtlasiO01803.

Expressioni

Tissue specificityi

Expressed weakly in sperm, but more predominantly in oocytes (PubMed:18472420). Expressed in the intestine (PubMed:24843160).2 Publications

Developmental stagei

Expressed during embryogenesis (PubMed:18385514, PubMed:20116245, PubMed:26506309). Transiently expressed during cytokinesis in embryos on the ingressing furrow and at the midbody during early abscission (PubMed:20116245). Expressed during the L4 stage and young adult stage of development (PubMed:22634595).4 Publications

Gene expression databases

BgeeiWBGene00004274.

Interactioni

Subunit structurei

Interacts with rei-1 and rei-2. The GDP-form preferentially binds to rei-1 and rei-2.1 Publication

Protein-protein interaction databases

DIPiDIP-24288N.
IntActiO01803. 1 interactor.
MINTiMINT-1111275.
STRINGi6239.F53G12.1.2.

Structurei

3D structure databases

ProteinModelPortaliO01803.
SMRiO01803.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi40 – 48Effector regionCurated9

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rab family.UniRule annotation

Phylogenomic databases

eggNOGiKOG0087. Eukaryota.
COG1100. LUCA.
GeneTreeiENSGT00760000118841.
HOGENOMiHOG000233968.
InParanoidiO01803.
KOiK07904.
OMAiETKQGCC.
OrthoDBiEOG091G0O1R.
PhylomeDBiO01803.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O01803-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSRDDEYDY LFKVVLIGDS GVGKSNLLSR FTRNEFNLES KSTIGVEFAT
60 70 80 90 100
RSISVEGKTV KAQIWDTAGQ ERYRAITSAY YRGAVGALLV YDIAKHVTYE
110 120 130 140 150
NVERWLKELR DHADQNIVIM LVGNKSDLRH LRAVPTDEAK IYAERNQLSF
160 170 180 190 200
IETSALDSTN VEAAFTNILT EIYKSVSNKH VGTDRQGYGG GSGTIIPSPA
210
SDPPKKQCCI P
Length:211
Mass (Da):23,429
Last modified:July 1, 1997 - v1
Checksum:iB0FD2837E42BE574
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
JQ235189 mRNA. Translation: AFP33153.1.
BX284601 Genomic DNA. Translation: CCD71701.1.
PIRiT29035.
RefSeqiNP_490675.1. NM_058274.3.
UniGeneiCel.17482.

Genome annotation databases

EnsemblMetazoaiF53G12.1.1; F53G12.1.1; WBGene00004274.
F53G12.1.2; F53G12.1.2; WBGene00004274.
GeneIDi171601.
KEGGicel:CELE_F53G12.1.
UCSCiF53G12.1. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
JQ235189 mRNA. Translation: AFP33153.1.
BX284601 Genomic DNA. Translation: CCD71701.1.
PIRiT29035.
RefSeqiNP_490675.1. NM_058274.3.
UniGeneiCel.17482.

3D structure databases

ProteinModelPortaliO01803.
SMRiO01803.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-24288N.
IntActiO01803. 1 interactor.
MINTiMINT-1111275.
STRINGi6239.F53G12.1.2.

Proteomic databases

EPDiO01803.
PaxDbiI7EVK5.
O01803.
PeptideAtlasiO01803.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiF53G12.1.1; F53G12.1.1; WBGene00004274.
F53G12.1.2; F53G12.1.2; WBGene00004274.
GeneIDi171601.
KEGGicel:CELE_F53G12.1.
UCSCiF53G12.1. c. elegans.

Organism-specific databases

CTDi171601.
WormBaseiF53G12.1; CE11006; WBGene00004274; rab-11.1.

Phylogenomic databases

eggNOGiKOG0087. Eukaryota.
COG1100. LUCA.
GeneTreeiENSGT00760000118841.
HOGENOMiHOG000233968.
InParanoidiO01803.
KOiK07904.
OMAiETKQGCC.
OrthoDBiEOG091G0O1R.
PhylomeDBiO01803.

Enzyme and pathway databases

ReactomeiR-CEL-5620912. Anchoring of the basal body to the plasma membrane.
R-CEL-5620916. VxPx cargo-targeting to cilium.

Miscellaneous databases

PROiO01803.

Gene expression databases

BgeeiWBGene00004274.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRB11A_CAEEL
AccessioniPrimary (citable) accession number: O01803
Secondary accession number(s): I7EVK5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 16, 2016
Last sequence update: July 1, 1997
Last modified: November 30, 2016
This is version 137 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.