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Protein

Spermatocyte protein spe-8

Gene

spe-8

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable non-receptor tyrosine-protein kinase which plays a role in spermatid activation (spermiogenesis) in hermaphrodites.2 Publications

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei250 – 2501ATPPROSITE-ProRule annotation
Active sitei344 – 3441Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi223 – 2319ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

  • spermatid development Source: WormBase
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase, Tyrosine-protein kinase

Keywords - Biological processi

Differentiation, Spermatogenesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

SignaLinkiO01798.

Names & Taxonomyi

Protein namesi
Recommended name:
Spermatocyte protein spe-8Curated (EC:2.7.10.2)
Alternative name(s):
Defective spermatogenesis protein spe-8Imported
Gene namesi
Name:spe-8Imported
ORF Names:F53G12.6Imported
OrganismiCaenorhabditis elegansImported
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome I

Organism-specific databases

WormBaseiF53G12.6; CE29331; WBGene00004962; spe-8.

Subcellular locationi

  • Cell membrane 1 Publication; Peripheral membrane protein 1 Publication
  • Cytoplasm 1 Publication

  • Note: Localizes mainly in the cytoplasm of stage I spermatocytes and at the cell membrane of stage II spermatocytes and spermatids.1 Publication

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • plasma membrane Source: WormBase
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi150 – 1501S → N in hc134ts; temperature-sensitive self-sterility in hermaphrodites but normal male fertility. 1 Publication
Mutagenesisi229 – 2291G → R in hc79; self-sterility in hermaphrodites but normal male fertility. 1 Publication
Mutagenesisi231 – 2311V → M in hc53; self-sterility in hermaphrodites but normal male fertility. 1 Publication
Mutagenesisi405 – 4051G → D in hc108; self-sterility in hermaphrodites but normal male fertility. 1 Publication
Mutagenesisi465 – 4651R → H in hc40; self-sterility in hermaphrodites but normal male fertility. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 512512Spermatocyte protein spe-8CuratedPRO_0000433988Add
BLAST

Proteomic databases

PaxDbiO01798.
PRIDEiO01798.

Expressioni

Tissue specificityi

Expression is restricted to male germline.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi6239.F53G12.6.

Structurei

3D structure databases

ProteinModelPortaliO01798.
SMRiO01798. Positions 113-477.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini114 – 20592SH2PROSITE-ProRule annotationAdd
BLAST
Domaini217 – 485269Protein kinasePROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi57 – 7822Pro-richPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation
Contains 1 SH2 domain.PROSITE-ProRule annotation

Keywords - Domaini

SH2 domain

Phylogenomic databases

eggNOGiKOG0194. Eukaryota.
ENOG410Y6RP. LUCA.
GeneTreeiENSGT00760000119011.
HOGENOMiHOG000017689.
InParanoidiO01798.
OMAiHEQIERN.
OrthoDBiEOG7P2XWX.
PhylomeDBiO01798.

Family and domain databases

Gene3Di3.30.505.10. 1 hit.
InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR000980. SH2.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
[Graphical view]
PfamiPF07714. Pkinase_Tyr. 1 hit.
PF00017. SH2. 1 hit.
[Graphical view]
PRINTSiPR00109. TYRKINASE.
SMARTiSM00252. SH2. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMiSSF55550. SSF55550. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS50001. SH2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O01798-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRSKSSEGDL QPEDTQSRED KETTATYSED TKPETQKERN AALDNLAKTP
60 70 80 90 100
IQLVVQPTPL TPAITPCEAP PPPPPPKPSS DNNNSKRLKV KDQLIEVPSD
110 120 130 140 150
EVGRVENNID NFPFYHGFMG RNECEAMLSN HGDFLIRMTE IGKRVAYVIS
160 170 180 190 200
IKWKYQNIHV LVKRTKTKKL YWTKKYAFKS ICELIAYHKR NHKPIYEGMT
210 220 230 240 250
LICGLARHGW QLNNEQVTLN KKLGEGQFGE VHKGSLKTSV FAAPVTVAVK
260 270 280 290 300
TLHQNHLSAN EKILFLREAN VMLTLSHPNV IKFYGVCTMK EPIMIVMEFC
310 320 330 340 350
DGKSLEDALL SKEEKVSAED KILYLFHAAC GIDYLHGKQV IHRDIAARNC
360 370 380 390 400
LLNSKKILKI SDFGLSVKGV AIKERKGGCL PVKYMAPETL KKGLYSTASD
410 420 430 440 450
IYSYGALMYE VYTDGKTPFE TCGLRGNELR KAIIGKRISL AVEVELPVFI
460 470 480 490 500
ANIFEQSRQY ETEDRISSKQ IIQIFKEEVG FHEIETSGIL HKLVNSLPRI
510
HNKERKPAAV AV
Length:512
Mass (Da):57,802
Last modified:December 1, 2001 - v2
Checksum:iE6B44EB28C1D7EB1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX284601 Genomic DNA. Translation: CCD71705.1.
PIRiT29030.
RefSeqiNP_490680.1. NM_058279.1.
UniGeneiCel.134.

Genome annotation databases

EnsemblMetazoaiF53G12.6; F53G12.6; WBGene00004962.
GeneIDi171606.
KEGGicel:CELE_F53G12.6.
UCSCiF53G12.6. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX284601 Genomic DNA. Translation: CCD71705.1.
PIRiT29030.
RefSeqiNP_490680.1. NM_058279.1.
UniGeneiCel.134.

3D structure databases

ProteinModelPortaliO01798.
SMRiO01798. Positions 113-477.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi6239.F53G12.6.

Proteomic databases

PaxDbiO01798.
PRIDEiO01798.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiF53G12.6; F53G12.6; WBGene00004962.
GeneIDi171606.
KEGGicel:CELE_F53G12.6.
UCSCiF53G12.6. c. elegans.

Organism-specific databases

CTDi171606.
WormBaseiF53G12.6; CE29331; WBGene00004962; spe-8.

Phylogenomic databases

eggNOGiKOG0194. Eukaryota.
ENOG410Y6RP. LUCA.
GeneTreeiENSGT00760000119011.
HOGENOMiHOG000017689.
InParanoidiO01798.
OMAiHEQIERN.
OrthoDBiEOG7P2XWX.
PhylomeDBiO01798.

Enzyme and pathway databases

SignaLinkiO01798.

Miscellaneous databases

NextBioi871981.

Family and domain databases

Gene3Di3.30.505.10. 1 hit.
InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR000980. SH2.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
[Graphical view]
PfamiPF07714. Pkinase_Tyr. 1 hit.
PF00017. SH2. 1 hit.
[Graphical view]
PRINTSiPR00109. TYRKINASE.
SMARTiSM00252. SH2. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMiSSF55550. SSF55550. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS50001. SH2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "SPE-8, a protein-tyrosine kinase, localizes to the spermatid cell membrane through interaction with other members of the SPE-8 group spermatid activation signaling pathway in C. elegans."
    Muhlrad P.J., Clark J.N., Nasri U., Sullivan N.G., LaMunyon C.W.
    BMC Genet. 15:83-83(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2Imported.
  3. "Developmental genetics of chromosome I spermatogenesis-defective mutants in the nematode Caenorhabditis elegans."
    L'Hernault S.W., Shakes D.C., Ward S.
    Genetics 120:435-452(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF SER-150; GLY-229; VAL-231; GLY-405 AND ARG-465.

Entry informationi

Entry nameiSPE8_CAEEL
AccessioniPrimary (citable) accession number: O01798
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 14, 2015
Last sequence update: December 1, 2001
Last modified: May 11, 2016
This is version 136 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.