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Protein

Spermatocyte protein spe-8

Gene

spe-8

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable non-receptor tyrosine-protein kinase which plays a role in spermatid activation (spermiogenesis) in hermaphrodites.2 Publications

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei250ATPPROSITE-ProRule annotation1
Active sitei344Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi223 – 231ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase, Tyrosine-protein kinase

Keywords - Biological processi

Differentiation, Spermatogenesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

SignaLinkiO01798.

Names & Taxonomyi

Protein namesi
Recommended name:
Spermatocyte protein spe-8Curated (EC:2.7.10.2)
Alternative name(s):
Defective spermatogenesis protein spe-8Imported
Gene namesi
Name:spe-8Imported
ORF Names:F53G12.6Imported
OrganismiCaenorhabditis elegansImported
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome I

Organism-specific databases

WormBaseiF53G12.6; CE29331; WBGene00004962; spe-8.

Subcellular locationi

  • Cell membrane 1 Publication; Peripheral membrane protein 1 Publication
  • Cytoplasm 1 Publication

  • Note: Localizes mainly in the cytoplasm of stage I spermatocytes and at the cell membrane of stage II spermatocytes and spermatids.1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi150S → N in hc134ts; temperature-sensitive self-sterility in hermaphrodites but normal male fertility. 1 Publication1
Mutagenesisi229G → R in hc79; self-sterility in hermaphrodites but normal male fertility. 1 Publication1
Mutagenesisi231V → M in hc53; self-sterility in hermaphrodites but normal male fertility. 1 Publication1
Mutagenesisi405G → D in hc108; self-sterility in hermaphrodites but normal male fertility. 1 Publication1
Mutagenesisi465R → H in hc40; self-sterility in hermaphrodites but normal male fertility. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004339881 – 512Spermatocyte protein spe-8CuratedAdd BLAST512

Proteomic databases

PaxDbiO01798.

Expressioni

Tissue specificityi

Expression is restricted to male germline.1 Publication

Gene expression databases

BgeeiWBGene00004962.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

STRINGi6239.F53G12.6.

Structurei

3D structure databases

ProteinModelPortaliO01798.
SMRiO01798.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini114 – 205SH2PROSITE-ProRule annotationAdd BLAST92
Domaini217 – 485Protein kinasePROSITE-ProRule annotationAdd BLAST269

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi57 – 78Pro-richPROSITE-ProRule annotationAdd BLAST22

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation
Contains 1 SH2 domain.PROSITE-ProRule annotation

Keywords - Domaini

SH2 domain

Phylogenomic databases

eggNOGiKOG0194. Eukaryota.
ENOG410Y6RP. LUCA.
GeneTreeiENSGT00760000119011.
HOGENOMiHOG000017689.
InParanoidiO01798.
OMAiHEQIERN.
OrthoDBiEOG091G08FJ.
PhylomeDBiO01798.

Family and domain databases

Gene3Di3.30.505.10. 1 hit.
InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR000980. SH2.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
[Graphical view]
PfamiPF07714. Pkinase_Tyr. 1 hit.
PF00017. SH2. 1 hit.
[Graphical view]
PRINTSiPR00109. TYRKINASE.
SMARTiSM00252. SH2. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMiSSF55550. SSF55550. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS50001. SH2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O01798-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRSKSSEGDL QPEDTQSRED KETTATYSED TKPETQKERN AALDNLAKTP
60 70 80 90 100
IQLVVQPTPL TPAITPCEAP PPPPPPKPSS DNNNSKRLKV KDQLIEVPSD
110 120 130 140 150
EVGRVENNID NFPFYHGFMG RNECEAMLSN HGDFLIRMTE IGKRVAYVIS
160 170 180 190 200
IKWKYQNIHV LVKRTKTKKL YWTKKYAFKS ICELIAYHKR NHKPIYEGMT
210 220 230 240 250
LICGLARHGW QLNNEQVTLN KKLGEGQFGE VHKGSLKTSV FAAPVTVAVK
260 270 280 290 300
TLHQNHLSAN EKILFLREAN VMLTLSHPNV IKFYGVCTMK EPIMIVMEFC
310 320 330 340 350
DGKSLEDALL SKEEKVSAED KILYLFHAAC GIDYLHGKQV IHRDIAARNC
360 370 380 390 400
LLNSKKILKI SDFGLSVKGV AIKERKGGCL PVKYMAPETL KKGLYSTASD
410 420 430 440 450
IYSYGALMYE VYTDGKTPFE TCGLRGNELR KAIIGKRISL AVEVELPVFI
460 470 480 490 500
ANIFEQSRQY ETEDRISSKQ IIQIFKEEVG FHEIETSGIL HKLVNSLPRI
510
HNKERKPAAV AV
Length:512
Mass (Da):57,802
Last modified:December 1, 2001 - v2
Checksum:iE6B44EB28C1D7EB1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX284601 Genomic DNA. Translation: CCD71705.1.
PIRiT29030.
RefSeqiNP_490680.1. NM_058279.1.
UniGeneiCel.134.

Genome annotation databases

EnsemblMetazoaiF53G12.6; F53G12.6; WBGene00004962.
GeneIDi171606.
KEGGicel:CELE_F53G12.6.
UCSCiF53G12.6. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX284601 Genomic DNA. Translation: CCD71705.1.
PIRiT29030.
RefSeqiNP_490680.1. NM_058279.1.
UniGeneiCel.134.

3D structure databases

ProteinModelPortaliO01798.
SMRiO01798.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi6239.F53G12.6.

Proteomic databases

PaxDbiO01798.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiF53G12.6; F53G12.6; WBGene00004962.
GeneIDi171606.
KEGGicel:CELE_F53G12.6.
UCSCiF53G12.6. c. elegans.

Organism-specific databases

CTDi171606.
WormBaseiF53G12.6; CE29331; WBGene00004962; spe-8.

Phylogenomic databases

eggNOGiKOG0194. Eukaryota.
ENOG410Y6RP. LUCA.
GeneTreeiENSGT00760000119011.
HOGENOMiHOG000017689.
InParanoidiO01798.
OMAiHEQIERN.
OrthoDBiEOG091G08FJ.
PhylomeDBiO01798.

Enzyme and pathway databases

SignaLinkiO01798.

Miscellaneous databases

PROiO01798.

Gene expression databases

BgeeiWBGene00004962.

Family and domain databases

Gene3Di3.30.505.10. 1 hit.
InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR000980. SH2.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
[Graphical view]
PfamiPF07714. Pkinase_Tyr. 1 hit.
PF00017. SH2. 1 hit.
[Graphical view]
PRINTSiPR00109. TYRKINASE.
SMARTiSM00252. SH2. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMiSSF55550. SSF55550. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS50001. SH2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSPE8_CAEEL
AccessioniPrimary (citable) accession number: O01798
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 14, 2015
Last sequence update: December 1, 2001
Last modified: November 2, 2016
This is version 141 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.