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Reviewed, UniProtKB/Swiss-Prot O01705 (EXT2_CAEEL)

Last modified November 3, 2009. Version 64. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Exostosin-2
    EC=2.4.1.223
    EC=2.4.1.224
Alternative name(s):
    Glucuronyl-galactosyl-proteoglycan/Glucuronosyl-N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase
    Multiple exostoses homolog 2
Gene names
Name: rib-2
ORF Names: K01G5.6
OrganismCaenorhabditis elegans [Complete proteome]
Taxonomic identifier6239 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

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Protein attributes

Sequence length814 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at transcript level.

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General annotation (Comments)

Function

Probable alpha1,4-N-acetylglucosaminyltransferase required for the biosynthesis of heparan-sulfate By similarity.

Catalytic activity

UDP-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1->3)-beta-D-galactosyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-xylosyl-proteoglycan = UDP + alpha-N-acetyl-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-beta-D-galactosyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-xylosyl-proteoglycan.

UDP-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan = UDP + N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan.

Pathway

Glycan metabolism; heparan sulfate biosynthesis.

Subcellular location

Endoplasmic reticulum membrane; Single-pass type II membrane protein By similarity.

Sequence similarities

Belongs to the glycosyltransferase 47 family.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 814814Exostosin-2
PRO_0000149663

Regions

Topological domain1 – 1414Cytoplasmic Potential
Transmembrane15 – 3521Signal-anchor for type II membrane protein Potential
Topological domain36 – 814779Lumenal Potential

Amino acid modifications

Glycosylation361N-linked (GlcNAc...) Potential
Glycosylation2271N-linked (GlcNAc...) Potential
Glycosylation2971N-linked (GlcNAc...) Potential
Glycosylation3221N-linked (GlcNAc...) Potential
Glycosylation4541N-linked (GlcNAc...) Potential
Glycosylation4921N-linked (GlcNAc...) Potential
Glycosylation6851N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict6041T → S in AAC47510. Ref.1

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Sequences

Sequence LengthMass (Da)Tools
O01705-1 [UniParc].

Last modified March 27, 2002. Version 2.
Checksum: 38FA58C5EB17DB16

FASTA81494,197
        10         20         30         40         50         60 
MAIKLNGSSR SFVPSLRVSA FLIFIFFVIT YIIIYNVSFS EPSWITQDAL KQNIENLDDY 

        70         80         90        100        110        120 
DASCSGYSIG RILREQKRIL ASVRLELTES QVKIEEIRTV QEELQRLIPQ KQLELSALEG 

       130        140        150        160        170        180 
EIEAAQRQLE ELRETQNVKV FLPFSPLQIP RELEQPSQIS PNQLDDIIDY SRCSISSFMP 

       190        200        210        220        230        240 
VYVDIITSGQ SEKEWLNVFQ EVIPNLVETP DKACIKIHIS NGIASPNTTF NSILFNVGSP 

       250        260        270        280        290        300 
IINFQSKSIH VQASKIRSFD FPVDVNHIAV EKVDLTPLLP FQRENLISLI VDNTELNFSA 

       310        320        330        340        350        360 
FSSLSAEPSR RPIVIVKCSQ ENCSLERRRQ LIGSSTFCFL LPSEMFFQDF LSSLQLGCIP 

       370        380        390        400        410        420 
IILSNSQLLP FQDLIDWRRA TYRLPLARLP EAHFIVQSFE ISDIIEMRRV GRLFYETYLA 

       430        440        450        460        470        480 
DRHLLARSLL AALRYKLQIP TREVRRNQAI PLFNSSFTAP KGSVVNVQAN FDDEYLLGPL 

       490        500        510        520        530        540 
ESRVESTSYA YNFTEFQLYS YDFWNIIMSP HYTKEFLVNA AELPTEAEFF EDTKIGFRPI 

       550        560        570        580        590        600 
EPGSGAEFSK ALGGNRQREQ FTVVLLTYER DAVLTGALER LHQLPYLNKI IVVWNNVNRD 

       610        620        630        640        650        660 
PPDTWPSLHI PVEFIRVAEN NLNNRFVPWD RIETEAVLSL DDDIDLMQQE IILAFRVWRE 

       670        680        690        700        710        720 
NRDRIVGFPA RHHARYGDSM FYNSNHTCQM SMILTGAAFI HKNYLTAYTY EMPAEIREHV 

       730        740        750        760        770        780 
NSIKNCEDIA MNYLVSHLTR KPPIKTTSRW TLKCPTCTES LYKEGTHFEK RHECMRLFTK 

       790        800        810 
IYGYNPLKFS QFRADSILFK TRLPQNHQKC FKYV

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References

« Hide 'large scale' references
[1]"The structure of the human multiple exostoses 2 gene and characterization of homologs in mouse and Caenorhabditis elegans."
Clines G.A., Ashley J.A., Shah S., Lovett M.
Genome Res. 7:359-367(1997) [PubMed: 9110175] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"rib-2, a Caenorhabditis elegans homolog of the human tumor suppressor EXT genes encodes a novel alpha1,4-N-acetylglucosaminyltransferase involved in the biosynthetic initiation and elongation of heparan sulfate."
Kitagawa H., Egusa N., Tamura J., Kusche-Gullberg M., Lindahl U., Sugahara K.
J. Biol. Chem. 276:4834-4838(2001) [PubMed: 11121397] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Genome sequence of the nematode C. elegans: a platform for investigating biology."
The C. elegans sequencing consortium
Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Bristol N2.
+Additional computationally mapped references.

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Cross-references

Sequence databases

U94835 mRNA. Translation: AAC47510.1.
AB077851 mRNA. Translation: BAB83878.1.
Z92803 Genomic DNA. Translation: CAB07245.1.
PIRT23200.
RefSeqNP_499368.1.
UniGeneCel.17904

3D structure databases

HSSPHSSP built from PDB template 1OMX based on UniProtKB Q9ES89.
ModBaseSearch...

Protein-protein interaction databases

STRINGO01705.

Protein family/group databases

CAZyGT47. Glycosyltransferase Family 47.
GT64. Glycosyltransferase Family 64.

Genome annotation databases

EnsemblK01G5.6; K01G5.6; K01G5.6; Caenorhabditis elegans. [Genome view]
GeneID176502.
KEGGcel:K01G5.6.
NMPDRfig|6239.3.peg.11542.
UCSCK01G5.6. c. elegans.

Organism-specific databases

CTD176502.
WormBaseWBGene00004361. rib-2.
WormPepK01G5.6. CE16196. [WorfDB]

Phylogenomic databases

OMAWDRIETE.

Enzyme and pathway databases

BRENDA2.4.1.223. 672.
2.4.1.224. 672.

Gene expression databases

ArrayExpressO01705.

Family and domain databases

InterProIPR004263. Exostosin.
IPR015338. HexNAc_Trfase_a.
[Graphical view]
PfamPF03016. Exostosin. 1 hit.
PF09258. Glyco_transf_64. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio892848.

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Entry information

Entry nameEXT2_CAEEL
AccessionPrimary (citable) accession number: O01705
Secondary accession number(s): O17920
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: March 27, 2002
Last modified: November 3, 2009
This is version 64 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectCaenorhabditis annotation project

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Relevant documents

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormPep

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents