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O01705

- EXT2_CAEEL

UniProt

O01705 - EXT2_CAEEL

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Protein
Exostosin-2
Gene
rib-2, K01G5.6
Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Probable alpha1,4-N-acetylglucosaminyltransferase required for the biosynthesis of heparan-sulfate By similarity.

Catalytic activityi

UDP-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1->3)-beta-D-galactosyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-xylosyl-proteoglycan = UDP + alpha-N-acetyl-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-beta-D-galactosyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-xylosyl-proteoglycan.
UDP-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan = UDP + N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan.

Pathwayi

GO - Molecular functioni

  1. acetylglucosaminyltransferase activity Source: WormBase
  2. enzyme binding Source: WormBase
  3. glucuronosyl-N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase activity Source: UniProtKB-EC
  4. glucuronyl-galactosyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase activity Source: UniProtKB-EC
  5. heparan sulfate N-acetylglucosaminyltransferase activity Source: WormBase
  6. protein binding Source: WormBase
Complete GO annotation...

GO - Biological processi

  1. embryonic morphogenesis Source: WormBase
  2. heparan sulfate proteoglycan biosynthetic process Source: WormBase
  3. pharynx development Source: WormBase
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

ReactomeiREACT_197732. XBP1(S) activates chaperone genes.
UniPathwayiUPA00756.

Protein family/group databases

CAZyiGT47. Glycosyltransferase Family 47.
GT64. Glycosyltransferase Family 64.

Names & Taxonomyi

Protein namesi
Recommended name:
Exostosin-2 (EC:2.4.1.223, EC:2.4.1.224)
Alternative name(s):
Glucuronyl-galactosyl-proteoglycan/Glucuronosyl-N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase
Multiple exostoses homolog 2
Gene namesi
Name:rib-2
ORF Names:K01G5.6
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940: Chromosome III

Organism-specific databases

WormBaseiK01G5.6; CE16196; WBGene00004361; rib-2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1414Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei15 – 3521Helical; Signal-anchor for type II membrane protein; Reviewed prediction
Add
BLAST
Topological domaini36 – 814779Lumenal Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. intrinsic component of endoplasmic reticulum membrane Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 814814Exostosin-2
PRO_0000149663Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi36 – 361N-linked (GlcNAc...) Reviewed prediction
Glycosylationi227 – 2271N-linked (GlcNAc...) Reviewed prediction
Glycosylationi297 – 2971N-linked (GlcNAc...) Reviewed prediction
Glycosylationi322 – 3221N-linked (GlcNAc...) Reviewed prediction
Glycosylationi454 – 4541N-linked (GlcNAc...) Reviewed prediction
Glycosylationi492 – 4921N-linked (GlcNAc...) Reviewed prediction
Glycosylationi685 – 6851N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiO01705.

Interactioni

Protein-protein interaction databases

STRINGi6239.K01G5.6.

Structurei

3D structure databases

ProteinModelPortaliO01705.
SMRiO01705. Positions 559-790.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG243446.
GeneTreeiENSGT00550000074496.
HOGENOMiHOG000007975.
InParanoidiO01705.
KOiK02370.
OMAiWDRIETE.
OrthoDBiEOG7PZRWK.
PhylomeDBiO01705.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR027674. EXO3/EXOL3.
IPR004263. Exostosin.
IPR015338. HexNAc_Trfase_a.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR11062. PTHR11062. 1 hit.
PTHR11062:SF57. PTHR11062:SF57. 1 hit.
PfamiPF03016. Exostosin. 1 hit.
PF09258. Glyco_transf_64. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

O01705-1 [UniParc]FASTAAdd to Basket

« Hide

MAIKLNGSSR SFVPSLRVSA FLIFIFFVIT YIIIYNVSFS EPSWITQDAL    50
KQNIENLDDY DASCSGYSIG RILREQKRIL ASVRLELTES QVKIEEIRTV 100
QEELQRLIPQ KQLELSALEG EIEAAQRQLE ELRETQNVKV FLPFSPLQIP 150
RELEQPSQIS PNQLDDIIDY SRCSISSFMP VYVDIITSGQ SEKEWLNVFQ 200
EVIPNLVETP DKACIKIHIS NGIASPNTTF NSILFNVGSP IINFQSKSIH 250
VQASKIRSFD FPVDVNHIAV EKVDLTPLLP FQRENLISLI VDNTELNFSA 300
FSSLSAEPSR RPIVIVKCSQ ENCSLERRRQ LIGSSTFCFL LPSEMFFQDF 350
LSSLQLGCIP IILSNSQLLP FQDLIDWRRA TYRLPLARLP EAHFIVQSFE 400
ISDIIEMRRV GRLFYETYLA DRHLLARSLL AALRYKLQIP TREVRRNQAI 450
PLFNSSFTAP KGSVVNVQAN FDDEYLLGPL ESRVESTSYA YNFTEFQLYS 500
YDFWNIIMSP HYTKEFLVNA AELPTEAEFF EDTKIGFRPI EPGSGAEFSK 550
ALGGNRQREQ FTVVLLTYER DAVLTGALER LHQLPYLNKI IVVWNNVNRD 600
PPDTWPSLHI PVEFIRVAEN NLNNRFVPWD RIETEAVLSL DDDIDLMQQE 650
IILAFRVWRE NRDRIVGFPA RHHARYGDSM FYNSNHTCQM SMILTGAAFI 700
HKNYLTAYTY EMPAEIREHV NSIKNCEDIA MNYLVSHLTR KPPIKTTSRW 750
TLKCPTCTES LYKEGTHFEK RHECMRLFTK IYGYNPLKFS QFRADSILFK 800
TRLPQNHQKC FKYV 814
Length:814
Mass (Da):94,197
Last modified:March 27, 2002 - v2
Checksum:i38FA58C5EB17DB16
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti604 – 6041T → S in AAC47510. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U94835 mRNA. Translation: AAC47510.1.
AB077851 mRNA. Translation: BAB83878.1.
Z92803 Genomic DNA. Translation: CAB07245.1.
PIRiT23200.
RefSeqiNP_499368.1. NM_066967.6.
UniGeneiCel.17904.

Genome annotation databases

EnsemblMetazoaiK01G5.6; K01G5.6; WBGene00004361.
GeneIDi176502.
KEGGicel:CELE_K01G5.6.
UCSCiK01G5.6. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U94835 mRNA. Translation: AAC47510.1 .
AB077851 mRNA. Translation: BAB83878.1 .
Z92803 Genomic DNA. Translation: CAB07245.1 .
PIRi T23200.
RefSeqi NP_499368.1. NM_066967.6.
UniGenei Cel.17904.

3D structure databases

ProteinModelPortali O01705.
SMRi O01705. Positions 559-790.
ModBasei Search...

Protein-protein interaction databases

STRINGi 6239.K01G5.6.

Protein family/group databases

CAZyi GT47. Glycosyltransferase Family 47.
GT64. Glycosyltransferase Family 64.

Proteomic databases

PRIDEi O01705.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai K01G5.6 ; K01G5.6 ; WBGene00004361 .
GeneIDi 176502.
KEGGi cel:CELE_K01G5.6.
UCSCi K01G5.6. c. elegans.

Organism-specific databases

CTDi 176502.
WormBasei K01G5.6 ; CE16196 ; WBGene00004361 ; rib-2.

Phylogenomic databases

eggNOGi NOG243446.
GeneTreei ENSGT00550000074496.
HOGENOMi HOG000007975.
InParanoidi O01705.
KOi K02370.
OMAi WDRIETE.
OrthoDBi EOG7PZRWK.
PhylomeDBi O01705.

Enzyme and pathway databases

UniPathwayi UPA00756 .
Reactomei REACT_197732. XBP1(S) activates chaperone genes.

Miscellaneous databases

NextBioi 892848.
PROi O01705.

Family and domain databases

Gene3Di 3.90.550.10. 1 hit.
InterProi IPR027674. EXO3/EXOL3.
IPR004263. Exostosin.
IPR015338. HexNAc_Trfase_a.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view ]
PANTHERi PTHR11062. PTHR11062. 1 hit.
PTHR11062:SF57. PTHR11062:SF57. 1 hit.
Pfami PF03016. Exostosin. 1 hit.
PF09258. Glyco_transf_64. 1 hit.
[Graphical view ]
SUPFAMi SSF53448. SSF53448. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The structure of the human multiple exostoses 2 gene and characterization of homologs in mouse and Caenorhabditis elegans."
    Clines G.A., Ashley J.A., Shah S., Lovett M.
    Genome Res. 7:359-367(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "rib-2, a Caenorhabditis elegans homolog of the human tumor suppressor EXT genes encodes a novel alpha1,4-N-acetylglucosaminyltransferase involved in the biosynthetic initiation and elongation of heparan sulfate."
    Kitagawa H., Egusa N., Tamura J., Kusche-Gullberg M., Lindahl U., Sugahara K.
    J. Biol. Chem. 276:4834-4838(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.

Entry informationi

Entry nameiEXT2_CAEEL
AccessioniPrimary (citable) accession number: O01705
Secondary accession number(s): O17920
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: March 27, 2002
Last modified: September 3, 2014
This is version 103 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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