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O01705

- EXT2_CAEEL

UniProt

O01705 - EXT2_CAEEL

Protein

Exostosin-2

Gene

rib-2

Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 2 (27 Mar 2002)
      Previous versions | rss
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    Functioni

    Probable alpha1,4-N-acetylglucosaminyltransferase required for the biosynthesis of heparan-sulfate.By similarity

    Catalytic activityi

    UDP-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1->3)-beta-D-galactosyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-xylosyl-proteoglycan = UDP + alpha-N-acetyl-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-beta-D-galactosyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-xylosyl-proteoglycan.
    UDP-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan = UDP + N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan.

    Pathwayi

    GO - Molecular functioni

    1. acetylglucosaminyltransferase activity Source: WormBase
    2. enzyme binding Source: WormBase
    3. glucuronosyl-N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase activity Source: UniProtKB-EC
    4. glucuronyl-galactosyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase activity Source: UniProtKB-EC
    5. heparan sulfate N-acetylglucosaminyltransferase activity Source: WormBase
    6. protein binding Source: WormBase

    GO - Biological processi

    1. embryonic morphogenesis Source: WormBase
    2. heparan sulfate proteoglycan biosynthetic process Source: WormBase
    3. pharynx development Source: WormBase

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Enzyme and pathway databases

    ReactomeiREACT_197732. XBP1(S) activates chaperone genes.
    UniPathwayiUPA00756.

    Protein family/group databases

    CAZyiGT47. Glycosyltransferase Family 47.
    GT64. Glycosyltransferase Family 64.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Exostosin-2 (EC:2.4.1.223, EC:2.4.1.224)
    Alternative name(s):
    Glucuronyl-galactosyl-proteoglycan/Glucuronosyl-N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase
    Multiple exostoses homolog 2
    Gene namesi
    Name:rib-2
    ORF Names:K01G5.6
    OrganismiCaenorhabditis elegans
    Taxonomic identifieri6239 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
    ProteomesiUP000001940: Chromosome III

    Organism-specific databases

    WormBaseiK01G5.6; CE16196; WBGene00004361; rib-2.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. intrinsic component of endoplasmic reticulum membrane Source: InterPro

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 814814Exostosin-2PRO_0000149663Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi36 – 361N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi227 – 2271N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi297 – 2971N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi322 – 3221N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi454 – 4541N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi492 – 4921N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi685 – 6851N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PRIDEiO01705.

    Interactioni

    Protein-protein interaction databases

    BioGridi41689. 1 interaction.
    STRINGi6239.K01G5.6.

    Structurei

    3D structure databases

    ProteinModelPortaliO01705.
    SMRiO01705. Positions 559-790.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 1414CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini36 – 814779LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei15 – 3521Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyltransferase 47 family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG243446.
    GeneTreeiENSGT00550000074496.
    HOGENOMiHOG000007975.
    InParanoidiO01705.
    KOiK02370.
    OMAiWDRIETE.
    OrthoDBiEOG7PZRWK.
    PhylomeDBiO01705.

    Family and domain databases

    Gene3Di3.90.550.10. 1 hit.
    InterProiIPR027674. EXO3/EXOL3.
    IPR004263. Exostosin.
    IPR015338. HexNAc_Trfase_a.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view]
    PANTHERiPTHR11062. PTHR11062. 1 hit.
    PTHR11062:SF57. PTHR11062:SF57. 1 hit.
    PfamiPF03016. Exostosin. 1 hit.
    PF09258. Glyco_transf_64. 1 hit.
    [Graphical view]
    SUPFAMiSSF53448. SSF53448. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    O01705-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAIKLNGSSR SFVPSLRVSA FLIFIFFVIT YIIIYNVSFS EPSWITQDAL    50
    KQNIENLDDY DASCSGYSIG RILREQKRIL ASVRLELTES QVKIEEIRTV 100
    QEELQRLIPQ KQLELSALEG EIEAAQRQLE ELRETQNVKV FLPFSPLQIP 150
    RELEQPSQIS PNQLDDIIDY SRCSISSFMP VYVDIITSGQ SEKEWLNVFQ 200
    EVIPNLVETP DKACIKIHIS NGIASPNTTF NSILFNVGSP IINFQSKSIH 250
    VQASKIRSFD FPVDVNHIAV EKVDLTPLLP FQRENLISLI VDNTELNFSA 300
    FSSLSAEPSR RPIVIVKCSQ ENCSLERRRQ LIGSSTFCFL LPSEMFFQDF 350
    LSSLQLGCIP IILSNSQLLP FQDLIDWRRA TYRLPLARLP EAHFIVQSFE 400
    ISDIIEMRRV GRLFYETYLA DRHLLARSLL AALRYKLQIP TREVRRNQAI 450
    PLFNSSFTAP KGSVVNVQAN FDDEYLLGPL ESRVESTSYA YNFTEFQLYS 500
    YDFWNIIMSP HYTKEFLVNA AELPTEAEFF EDTKIGFRPI EPGSGAEFSK 550
    ALGGNRQREQ FTVVLLTYER DAVLTGALER LHQLPYLNKI IVVWNNVNRD 600
    PPDTWPSLHI PVEFIRVAEN NLNNRFVPWD RIETEAVLSL DDDIDLMQQE 650
    IILAFRVWRE NRDRIVGFPA RHHARYGDSM FYNSNHTCQM SMILTGAAFI 700
    HKNYLTAYTY EMPAEIREHV NSIKNCEDIA MNYLVSHLTR KPPIKTTSRW 750
    TLKCPTCTES LYKEGTHFEK RHECMRLFTK IYGYNPLKFS QFRADSILFK 800
    TRLPQNHQKC FKYV 814
    Length:814
    Mass (Da):94,197
    Last modified:March 27, 2002 - v2
    Checksum:i38FA58C5EB17DB16
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti604 – 6041T → S in AAC47510. (PubMed:9110175)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U94835 mRNA. Translation: AAC47510.1.
    AB077851 mRNA. Translation: BAB83878.1.
    Z92803 Genomic DNA. Translation: CAB07245.1.
    PIRiT23200.
    RefSeqiNP_499368.1. NM_066967.6.
    UniGeneiCel.17904.

    Genome annotation databases

    EnsemblMetazoaiK01G5.6; K01G5.6; WBGene00004361.
    GeneIDi176502.
    KEGGicel:CELE_K01G5.6.
    UCSCiK01G5.6. c. elegans.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U94835 mRNA. Translation: AAC47510.1 .
    AB077851 mRNA. Translation: BAB83878.1 .
    Z92803 Genomic DNA. Translation: CAB07245.1 .
    PIRi T23200.
    RefSeqi NP_499368.1. NM_066967.6.
    UniGenei Cel.17904.

    3D structure databases

    ProteinModelPortali O01705.
    SMRi O01705. Positions 559-790.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 41689. 1 interaction.
    STRINGi 6239.K01G5.6.

    Protein family/group databases

    CAZyi GT47. Glycosyltransferase Family 47.
    GT64. Glycosyltransferase Family 64.

    Proteomic databases

    PRIDEi O01705.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai K01G5.6 ; K01G5.6 ; WBGene00004361 .
    GeneIDi 176502.
    KEGGi cel:CELE_K01G5.6.
    UCSCi K01G5.6. c. elegans.

    Organism-specific databases

    CTDi 176502.
    WormBasei K01G5.6 ; CE16196 ; WBGene00004361 ; rib-2.

    Phylogenomic databases

    eggNOGi NOG243446.
    GeneTreei ENSGT00550000074496.
    HOGENOMi HOG000007975.
    InParanoidi O01705.
    KOi K02370.
    OMAi WDRIETE.
    OrthoDBi EOG7PZRWK.
    PhylomeDBi O01705.

    Enzyme and pathway databases

    UniPathwayi UPA00756 .
    Reactomei REACT_197732. XBP1(S) activates chaperone genes.

    Miscellaneous databases

    NextBioi 892848.
    PROi O01705.

    Family and domain databases

    Gene3Di 3.90.550.10. 1 hit.
    InterProi IPR027674. EXO3/EXOL3.
    IPR004263. Exostosin.
    IPR015338. HexNAc_Trfase_a.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view ]
    PANTHERi PTHR11062. PTHR11062. 1 hit.
    PTHR11062:SF57. PTHR11062:SF57. 1 hit.
    Pfami PF03016. Exostosin. 1 hit.
    PF09258. Glyco_transf_64. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53448. SSF53448. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The structure of the human multiple exostoses 2 gene and characterization of homologs in mouse and Caenorhabditis elegans."
      Clines G.A., Ashley J.A., Shah S., Lovett M.
      Genome Res. 7:359-367(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "rib-2, a Caenorhabditis elegans homolog of the human tumor suppressor EXT genes encodes a novel alpha1,4-N-acetylglucosaminyltransferase involved in the biosynthetic initiation and elongation of heparan sulfate."
      Kitagawa H., Egusa N., Tamura J., Kusche-Gullberg M., Lindahl U., Sugahara K.
      J. Biol. Chem. 276:4834-4838(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
      The C. elegans sequencing consortium
      Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Bristol N2.

    Entry informationi

    Entry nameiEXT2_CAEEL
    AccessioniPrimary (citable) accession number: O01705
    Secondary accession number(s): O17920
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: March 27, 2002
    Last modified: October 1, 2014
    This is version 104 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programCaenorhabditis annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Caenorhabditis elegans
      Caenorhabditis elegans: entries, gene names and cross-references to WormBase
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3