Skip Header

Contribute Send feedback
Read comments (0) or add your own

Reviewed, UniProtKB/Swiss-Prot O01700 (DLK1_CAEEL)

Last modified January 19, 2010. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Mitogen-activated protein kinase kinase kinase dlk-1
    EC=2.7.11.25
Alternative name(s):
    Death-associated protein kinase-like kinase
    DAP kinase-like kinase
Gene names
Name: dlk-1
ORF Names: F33E2.2
OrganismCaenorhabditis elegans [Complete proteome]
Taxonomic identifier6239 [NCBI]
Taxonomic lineageEukaryotaMetazoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis

Protein attributes

Sequence length928 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Component of a MAP kinase pathway that functions presynaptically to regulate synaptic architecture and presynaptic differentiation. Phosphorylates and activates mkk-4. Ref.2

Catalytic activity

ATP + a protein = ADP + a phosphoprotein. UniProtKB O43283

Cofactor

Magnesium By similarity. UniProtKB O43283

Subcellular location

Cell junctionsynapse Ref.2 Ref.4.

Tissue specificity

Expressed in neurons and pharynx. Ref.2

Post-translational modification

Ubiquitinated by rpm-1. Negatively regulated by ubiquitination by fsn-1 bound rpm-1, followed by degradation. Ref.2

Disruption phenotype

Worms overcome the lack of synaptogenesis caused by of the loss of rpm-1 ubiquitin ligase activity. Ref.3

Sequence similarities

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.

Contains 1 protein kinase domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

gei-16Q225341EBI-327135,EBI-327642
mig-5Q222271EBI-327135,EBI-316403

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform a Ref.1 (identifier: O01700-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: No experimental confirmation available.
Isoform b Ref.1 (identifier: O01700-2)

The sequence of this isoform differs from the canonical sequence as follows:
     674-681: GVISCSSP → A
Note: No experimental confirmation available.
Isoform c Ref.1 (identifier: O01700-3)

The sequence of this isoform differs from the canonical sequence as follows:
     567-577: KILRNDAIRHS → SEYIFPEKLNI
     578-928: Missing.
Note: No experimental confirmation available.
Isoform d Ref.1 (identifier: O01700-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-73: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 928928Mitogen-activated protein kinase kinase kinase dlk-1
PRO_0000353198

Regions

Domain135 – 377243Protein kinase
Nucleotide binding141 – 1499ATP By similarity UniProtKB O43283
Compositional bias530 – 55223Ser-rich

Sites

Active site2461Proton acceptor By similarity UniProtKB O43283
Binding site1621ATP By similarity UniProtKB O43283

Natural variations

Alternative sequence1 – 7373Missing in isoform d. Ref.1
VSP_052964
Alternative sequence567 – 57711KILRNDAIRHS → SEYIFPEKLNI in isoform c. Ref.1
VSP_052965
Alternative sequence578 – 928351Missing in isoform c. Ref.1
VSP_052966
Alternative sequence674 – 6818GVISCSSP → A in isoform b. Ref.1
VSP_052967

Sequences

Sequence LengthMass (Da)Tools
Isoform a [UniParc].

Last modified July 5, 2004. Version 4.
Checksum: EE071636ACAADBD4

FASTA928103,483
        10         20         30         40         50         60 
MTSTTMVTTL DLVTPTSEEQ PGPAPESSDF STVVLSPDGS ELVTQSAPNT PIQHREQANA 

        70         80         90        100        110        120 
EFGQKEGSPD PKNMVAATGN ASKPSLNSFY ADGLGQLRNG LFSCFQPVFG YFGTKTTVEI 

       130        140        150        160        170        180 
EKSEDELWEI PFDAISELEW LGSGSQGAVF RGQLENRTVA VKKVNQLKET EIKHLRHLRH 

       190        200        210        220        230        240 
QNIIEFLGVC SKSPCYCIVM EYCSKGQLCT VLKSRNTITR ELFAQWVKEI ADGMHYLHQN 

       250        260        270        280        290        300 
KVIHRDLKSP NILISAEDSI KICDFGTSHM QKKMDSTMMS FCGTVSWMAP EMIKKQPCNE 

       310        320        330        340        350        360 
KVDVYSFGVV LWEMLTRETP YANIAQMAII FGVGTNILSL PMPEEAPKGL VLLIKQCLSQ 

       370        380        390        400        410        420 
KGRNRPSFSH IRQHWEIFKP ELFEMTEEEW QLAWDSYREF AKCIQYPSTV TRDHGGPKSA 

       430        440        450        460        470        480 
FAMEEEIQRK RHEQLNHIKD IRNMYEMKLK RTNKMYDKLQ GCFTELKLKE SELAEWEKDL 

       490        500        510        520        530        540 
TEREQWHNQN SPKAVAAPRA QLRGYPNEGY DDMSSDEDVQ PCRGSPYRCS NTSSSSGVQS 

       550        560        570        580        590        600 
SPFSRQSSSR SSAGQQTRRS EGANPPKILR NDAIRHSGSY WETLGGARGS PARDSGFSQD 

       610        620        630        640        650        660 
SGMWSAGAGS CTAINGGGQQ VCYSQTLYRN GDGRWSDGRI ASRRRVSTSV NKSTAVPGQP 

       670        680        690        700        710        720 
VFFTRDSPSR VPHGVISCSS PRSSSKLNRS SYPSRNAPHQ LEDGCCCAHA RAPRAKSIAV 

       730        740        750        760        770        780 
PMTSSSRARS PTPYDNDFEN AESFVDPESP KNLKNLEKIV NLPESTSYDE ALCNSDVTMN 

       790        800        810        820        830        840 
PIYTSPITTY SNPCHVELVD EENANDVDLT SSMDSRRSRS DDADVESSEE DEGNGNNILN 

       850        860        870        880        890        900 
TSMESEDLRY RIDTSQSTMM SSLERSLEIG ATRSDGLSDN EMRVQAVKMS IKTHRRTGSN 

       910        920 
PQALIHQCID EYTTSATDDS DDAGAVRI 

« Hide

Isoform b.

Checksum: 302577E5324813B4
Show »

FASTA921102,824
Isoform c.

Checksum: BEDDDCCA10DB525A
Show »

FASTA57765,401
Isoform d.

Checksum: F44DD2538CB7D95A
Show »

FASTA85595,787

References

« Hide 'large scale' references
[1]"Genome sequence of the nematode C. elegans: a platform for investigating biology."
The C. elegans sequencing consortium
Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Bristol N2.
[2]"Regulation of a DLK-1 and p38 MAP kinase pathway by the ubiquitin ligase RPM-1 is required for presynaptic development."
Nakata K., Abrams B., Grill B., Goncharov A., Huang X., Chisholm A.D., Jin Y.
Cell 120:407-420(2005) [PubMed: 15707898] [Abstract]
Cited for: FUNCTION, ENZYME REGULATION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, UBIQUITINATION.
[3]"C. elegans RPM-1 regulates axon termination and synaptogenesis through the Rab GEF GLO-4 and the Rab GTPase GLO-1."
Grill B., Bienvenut W.V., Brown H.M., Ackley B.D., Quadroni M., Jin Y.
Neuron 55:587-601(2007) [PubMed: 17698012] [Abstract]
Cited for: ENZYME REGULATION, DISRUPTION PHENOTYPE.
[4]"Cellular and molecular determinants targeting the Caenorhabditis elegans PHR protein RPM-1 to perisynaptic regions."
Abrams B., Grill B., Huang X., Jin Y.
Dev. Dyn. 237:630-639(2008) [PubMed: 18224716] [Abstract]
Cited for: SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL022593, Z84574 Genomic DNA. Translation: CAA18635.3.
AL022593, Z84574 Genomic DNA. Translation: CAE54889.1.
Z84574, AL022593 Genomic DNA. Translation: CAB06544.3.
Z84574 Genomic DNA. Translation: CAE54890.1.
Z84574, AL022593 Genomic DNA. Translation: CAE54891.1.
AL022593, Z84574 Genomic DNA. Translation: CAQ76463.1.
Z84574, AL022593 Genomic DNA. Translation: CAQ76474.1.
PIRB87950.
T20082.
RefSeqNP_001021443.1.
NP_001021444.1.
NP_001021445.1.
NP_001129769.1.
UniGeneCel.24063

3D structure databases

HSSPHSSP built from PDB template 1UWJ based on UniProtKB P15056.
SMRO01700. Positions 60-267, 94-375.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-26475N.
IntActO01700. 2 interactions.

Proteomic databases

PRIDEO01700.

Genome annotation databases

EnsemblF33E2.2a; F33E2.2a; F33E2.2; Caenorhabditis elegans. [Genome view]
GeneID173128.
KEGGcel:F33E2.2.
UCSCF33E2.2b. c. elegans.

Organism-specific databases

CTD173128.
WormBaseWBGene00001008. dlk-1.
WormPepF33E2.2a. CE36153. [WorfDB]
F33E2.2b. CE36154. [WorfDB]
F33E2.2c. CE36155. [WorfDB]
F33E2.2d. CE23702. [WorfDB]

Phylogenomic databases

eggNOGmeNOG04663.
HOGENOMHBG378601.
InParanoidO01700.
OMASAFAMEE.
PhylomeDBO01700.

Gene expression databases

ArrayExpressO01700.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_cat_dom.
IPR017442. Se/Thr_prot_kinase-like_dom.
IPR008271. Ser/Thr_prot_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
PROSITEPS00107. PROTEIN_KINASE_ATP. False negative.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio878375.

Entry information

Entry nameDLK1_CAEEL
AccessionPrimary (citable) accession number: O01700
Secondary accession number(s): B3KYB4, Q7JKE7, Q7JKE9
Entry history
Integrated into UniProtKB/Swiss-Prot: November 4, 2008
Last sequence update: July 5, 2004
Last modified: January 19, 2010
This is version 90 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectCaenorhabditis annotation project

Relevant documents

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormPep

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents