O01668 (OPS6_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Opsin Rh6

Alternative name(s):
Rhodopsin Rh6, long-wavelength

Gene names

Name:	Rh6
ORF Names:	CG5192

Organism

Drosophila melanogaster (Fruit fly)

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	369 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Visual pigments are the light-absorbing molecules that mediate vision. They consist of an apoprotein, opsin, covalently linked to cis-retinal.
Subcellular location	Membrane; Multi-pass membrane protein.
Tissue specificity	Each Drosophila eye is composed of 800 facets or ommatidia. Each ommatidium contains 8 photoreceptor cells (R1-R8), the R1 to R6 cells are outer cells, while R7 and R8 are inner cells. Rh6 is expressed in a subset of R8 cells, most likely expressed in the subset of R8 cells paired with Rh4-expressing R7 cells (R7y).
Post-translational modification	Phosphorylated on some or all of the serine and threonine residues present in the C-terminal region By similarity.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.

Ontologies

Keywords
Biological process	Sensory transduction Vision
Cellular component	Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane Transmembrane helix
Ligand	Chromophore
Molecular function	G-protein coupled receptor Photoreceptor protein Receptor Retinal protein Transducer
PTM	Disulfide bond Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	phototransduction Inferred from genetic interaction. Source: FlyBase protein-chromophore linkage Inferred from electronic annotation. Source: UniProtKB-KW visual perception Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW rhabdomere Inferred from direct assay. Source: FlyBase
Molecular function	G-protein coupled photoreceptor activity Inferred from genetic interaction. Source: FlyBase
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform B (identifier: O01668-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform A (identifier: O01668-2)

The sequence of this isoform differs from the canonical sequence as follows:
     230-369: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 369	369	Opsin Rh6		PRO_0000197634
Regions
	Topological domain	1 – 46	46	Extracellular
	Transmembrane	47 – 71	25	Helical; Name=1; Potential
	Topological domain	72 – 83	12	Cytoplasmic
	Transmembrane	84 – 109	26	Helical; Name=2; Potential
	Topological domain	110 – 123	14	Extracellular
	Transmembrane	124 – 143	20	Helical; Name=3; Potential
	Topological domain	144 – 162	19	Cytoplasmic
	Transmembrane	163 – 186	24	Helical; Name=4; Potential
	Topological domain	187 – 210	24	Extracellular
	Transmembrane	211 – 238	28	Helical; Name=5; Potential
	Topological domain	239 – 274	36	Cytoplasmic
	Transmembrane	275 – 298	24	Helical; Name=6; Potential
	Topological domain	299 – 305	7	Extracellular
	Transmembrane	306 – 330	25	Helical; Name=7; Potential
	Topological domain	331 – 369	39	Cytoplasmic
Amino acid modifications
	Modified residue	317	1	N6-(retinylidene)lysine By similarity
	Glycosylation	17	1	N-linked (GlcNAc...) Potential
	Glycosylation	193	1	N-linked (GlcNAc...) Potential
	Glycosylation	208	1	N-linked (GlcNAc...) Potential
	Disulfide bond	120 ↔ 197		Potential
Natural variations
	Alternative sequence	230 – 369	140	Missing in isoform A.		VSP_009271

Sequences

	Sequence		Length	Mass (Da)
	Isoform B [UniParc]. Last modified July 1, 1997. Version 1. Checksum: 5670A7435AD5F244	FASTA	369	41,691
	10 20 30 40 50 60 MASLHPPSFA YMRDGRNLSL AESVPAEIMH MVDPYWYQWP PLEPMWFGII GFVIAILGTM 70 80 90 100 110 120 SLAGNFIVMY IFTSSKGLRT PSNMFVVNLA FSDFMMMFTM FPPVVLNGFY GTWIMGPFLC 130 140 150 160 170 180 ELYGMFGSLF GCVSIWSMTL IAYDRYCVIV KGMARKPLTA TAAVLRLMVV WTICGAWALM 190 200 210 220 230 240 PLFGWNRYVP EGNMTACGTD YFAKDWWNRS YIIVYSLWVY LTPLLTIIFS YWHIMKAVAA 250 260 270 280 290 300 HEKAMREQAK KMNVASLRNS EADKSKAIEI KLAKVALTTI SLWFFAWTPY TIINYAGIFE 310 320 330 340 350 360 SMHLSPLSTI CGSVFAKANA VCNPIVYGLS HPKYKQVLRE KMPCLACGKD DLTSDSRTQA TAEISESQA
	Isoform A [UniParc]. Checksum: EEB0CCDB777FDBEE	FASTA	229	26,194
	10 20 30 40 50 60 MASLHPPSFA YMRDGRNLSL AESVPAEIMH MVDPYWYQWP PLEPMWFGII GFVIAILGTM 70 80 90 100 110 120 SLAGNFIVMY IFTSSKGLRT PSNMFVVNLA FSDFMMMFTM FPPVVLNGFY GTWIMGPFLC 130 140 150 160 170 180 ELYGMFGSLF GCVSIWSMTL IAYDRYCVIV KGMARKPLTA TAAVLRLMVV WTICGAWALM 190 200 210 220 PLFGWNRYVP EGNMTACGTD YFAKDWWNRS YIIVYSLWVY LTPLLTIIF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of Drosophila Rh6 rhodopsin: the visual pigment of a subset of R8 photoreceptor cells." Huber A., Schulz S., Bentrop J., Groell C., Wolfrum U., Paulsen R. FEBS Lett. 406:6-10(1997) [PubMed: 9109375] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B). Strain: Oregon-R. Tissue: Retina.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[4]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A). Strain: Berkeley. Tissue: Head.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z86118 mRNA. Translation: CAB06821.1. AE014297 Genomic DNA. Translation: AAN13666.2. AY089666 mRNA. Translation: AAL90404.1.
RefSeq	NP_524368.3. NM_079644.2.
UniGene	Dm.1656.
3D structure databases
ProteinModelPortal	O01668.
SMR	O01668. Positions 31-354.
ModBase	Search...
Protein-protein interaction databases
STRING	O01668.
Protein family/group databases
GPCRDB	Search...
Proteomic databases
PRIDE	O01668.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0083146; FBpp0082600; FBgn0019940.
GeneID	41889.
KEGG	dme:Dmel_CG5192.
Organism-specific databases
CTD	41889.
FlyBase	FBgn0019940. Rh6.
Phylogenomic databases
eggNOG	inNOG09285.
InParanoid	O01668.
OrthoDB	EOG451C6G.
PhylomeDB	O01668.
Gene expression databases
Bgee	O01668.
Family and domain databases
InterPro	IPR000276. 7TM_GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_supfam. IPR001760. Opsin. IPR001735. Opsin_RH1/RH2. [Graphical view]
KO	K04255.
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00237. GPCRRHODOPSN. PR00238. OPSIN. PR00576. OPSINRH1RH2.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. PS00238. OPSIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	826121.

Entry information

Entry name

OPS6_DROME

Accession

Primary (citable) accession number: O01668
Secondary accession number(s): Q8SXF4, Q9VF58

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	July 1, 1997
Last modified:	January 25, 2012
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project