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Protein

Serine/threonine-protein kinase mrck-1

Gene

mrck-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Serine/threonine-protein kinase that may phosphorylate and inactivate the phosphatase mel-11, and thereby contribute to the regulation of myosin II contractility during embryonic elongation (PubMed:19675126). Involved in controlling canal length and Golgi/ER integrity during excretory canal elongation (PubMed:25743393).1 Publication1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.By similarity

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei112ATPPROSITE-ProRule annotation1
Active sitei207Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi89 – 97ATPPROSITE-ProRule annotation9
Zinc fingeri957 – 1007Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd BLAST51

GO - Molecular functioni

GO - Biological processi

  • embryo development ending in birth or egg hatching Source: WormBase
  • embryonic body morphogenesis Source: WormBase
  • embryonic morphogenesis Source: WormBase
  • epithelial tube morphogenesis Source: UniProtKB
  • intracellular signal transduction Source: InterPro
  • nematode larval development Source: WormBase
  • positive regulation of endocytic recycling Source: UniProtKB
  • positive regulation of GTPase activity Source: UniProtKB
  • regulation of Golgi organization Source: UniProtKB

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

SignaLinkiO01583

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase mrck-1Curated (EC:2.7.11.1By similarity)
Alternative name(s):
Myotonic dystrophy kinase-related CDC42-binding kinase homologImported
Gene namesi
Name:mrck-1Imported
ORF Names:K08B12.5Imported
OrganismiCaenorhabditis elegansImported
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome V

Organism-specific databases

WormBaseiK08B12.5 ; CE30818 ; WBGene00006437 ; mrck-1

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Normal adult development, but progeny arrest at either the L1 stage or during embryogenesis (PubMed:19675126). RNAi-mediated knockdown causes excretory canal truncation, abnormal lumen and cyst formation. In addition, causes a reduced distribution of Golgi and ER components along the excretory canal length and a decrease in cdc-42 activation (PubMed:25743393).2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004323831 – 1592Serine/threonine-protein kinase mrck-1Add BLAST1592

Proteomic databases

EPDiO01583
PaxDbiO01583
PeptideAtlasiO01583

Expressioni

Tissue specificityi

Expressed in embryonic and L4 larval seam cells and in embryonic dorsal and ventral epidermal cells. Also expressed in the pharynx throughout development and in sublateral nerve cords in the L4 larva.1 Publication

Gene expression databases

BgeeiWBGene00006437
ExpressionAtlasiO01583 baseline

Interactioni

Protein-protein interaction databases

DIPiDIP-25790N
IntActiO01583 1 interactor.
STRINGi6239.K08B12.5.2

Structurei

3D structure databases

ProteinModelPortaliO01583
SMRiO01583
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini83 – 351Protein kinasePROSITE-ProRule annotationAdd BLAST269
Domaini352 – 426AGC-kinase C-terminalSequence analysisAdd BLAST75
Domaini1026 – 1154PHPROSITE-ProRule annotationAdd BLAST129
Domaini1181 – 1479CNHPROSITE-ProRule annotationAdd BLAST299
Domaini1544 – 1557CRIBPROSITE-ProRule annotationAdd BLAST14

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili444 – 782Sequence analysisAdd BLAST339
Coiled coili811 – 871Sequence analysisAdd BLAST61

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi516 – 519Poly-GlnSequence analysis4

Sequence similaritiesi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri957 – 1007Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd BLAST51

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiKOG0612 Eukaryota
ENOG410XR1Q LUCA
GeneTreeiENSGT00760000118994
HOGENOMiHOG000294133
InParanoidiO01583
OMAiESEKSKW
OrthoDBiEOG091G09EX
PhylomeDBiO01583

Family and domain databases

CDDicd00029 C1, 1 hit
cd00132 CRIB, 1 hit
Gene3Di2.30.29.301 hit
InterProiView protein in InterPro
IPR000961 AGC-kinase_C
IPR001180 CNH_dom
IPR000095 CRIB_dom
IPR011009 Kinase-like_dom_sf
IPR002219 PE/DAG-bd
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00130 C1_1, 1 hit
PF00780 CNH, 1 hit
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00109 C1, 1 hit
SM00036 CNH, 1 hit
SM00285 PBD, 1 hit
SM00233 PH, 1 hit
SM00133 S_TK_X, 1 hit
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS51285 AGC_KINASE_CTER, 1 hit
PS50219 CNH, 1 hit
PS50108 CRIB, 1 hit
PS50003 PH_DOMAIN, 1 hit
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit
PS00479 ZF_DAG_PE_1, 1 hit
PS50081 ZF_DAG_PE_2, 1 hit

Sequencei

Sequence statusi: Complete.

O01583-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEPPPDDSA PVRLKTLENI YMDGPSKKPE ALSFETLIDS LICLYDECCN
60 70 80 90 100
STLRKEKCIA EFVESVKTVI SKAKKLRLSR DDFEVLKVIG KGAFGEVAVV
110 120 130 140 150
RMRGVGEIYA MKILNKWEMV KRAETACFRE ERDVLVYGDR RWITNLHYAF
160 170 180 190 200
QDEKNLYFVM DYYIGGDMLT LLSKFVDHIP ESMAKFYIAE MVLAIDSLHR
210 220 230 240 250
LGYVHRDVKP DNVLLDMQGH IRLADFGSCL RILADGSVAS NVAVGTPDYI
260 270 280 290 300
SPEILRAMED GRGRYGKECD WWSLGICMYE MLYGTTPFYS ERLVDTYGKI
310 320 330 340 350
MSHQDMLDFP DDEIDWVVSE EAKDLIRQLI CSSDVRFGRN GLSDFQLHPF
360 370 380 390 400
FEGIDWNTIR DSNPPYVPEV SSPEDTSNFD VDVCEDDFTP CLQETQPPRV
410 420 430 440 450
LAAFTGNHLP FVGFSYTHGS LLSDARSLTD EIRAIAQRCQ GDAELMEKSV
460 470 480 490 500
DGFMVELENE KAELVQKLKE AQTIIAQHVA ENPRSEEDRN YESTIAQLKD
510 520 530 540 550
EIQILNKRLE DEALAQQQQK PKDEIVAESE KKLKELKERN KQLVMEKSEI
560 570 580 590 600
QRELDNINDH LDQVLVEKAT VVQQRDDMQA ELADVGDSLL TEKDSVKRLQ
610 620 630 640 650
DEAEKAKKQV ADFEEKLKEI ETEKIALIKK QEEVTIEARK SVETDDHLSE
660 670 680 690 700
EVVAAKNTIA SLQATNEERE TEIKKLKQRM DEERASHTAQ SEQEMKQLEA
710 720 730 740 750
HYERAQKMLQ DNVEQMNVEN RGLRDEIEKL SQQMAALPRG GLNEQQLHEI
760 770 780 790 800
FNWVSEEKAT REEMENLTRK ITGEVESLKN NSPLTTSNYI QNTPSGWGSR
810 820 830 840 850
RMNNVARKDG LDLQRQLQAE IDAKLKLKAE LKNSQEQYLT SAARLDDTEK
860 870 880 890 900
RMASLMREVA MLKQQKNIEN SSDSAFSSTM GRGDLMISMN NDYEMSNSSL
910 920 930 940 950
MRQEMISRQS TPSYENAILL HDHQVPKRVD DLRYKQKPMK TASGIFSPVS
960 970 980 990 1000
ISAMERGHNF ERMKIKTPTK CGHCTSILIG LDRQGLFCQS CQYACHVSCA
1010 1020 1030 1040 1050
ERVSQSCPVP EEERRPLGID PTRGVGTAYE GLVKTPRAGG VRKGWQTAYV
1060 1070 1080 1090 1100
VVCDFKLYLY DCTVDRQNKM QDVKNEIRLV LDMRDPDFTV CGVSEADVIH
1110 1120 1130 1140 1150
AQKGDIPKIF RVTTTQILNS SSEYSSSSKF YTLFMAETEE EKRKWVVALS
1160 1170 1180 1190 1200
ELKTLLRRSK LADRKAFLVK EVFDVTTLPS IRVAQCCAII DRSKIVIGFS
1210 1220 1230 1240 1250
DHGLYCIEIS RQLLIPVGGE KENKQRCVET VEYDEAEQLL MMIVGPAKDR
1260 1270 1280 1290 1300
HVRIVPSAAL DGRDLKWIKV NDTKGCHLLA VGTNNPGGRA GFFAVAFKKS
1310 1320 1330 1340 1350
VTIFQIDRSE KRHKKWKDLA MPGTPQSIAI FNGRLYVGFS HSFRSWSLVG
1360 1370 1380 1390 1400
VDSSPVGSGD ASGAVLQHIS LVNMEDTSLQ FLNQQTSYEA KLIVNVPGSP
1410 1420 1430 1440 1450
DEYLLVFNMI GLYVNEMGRR SRLPEVMFPT QAKYFAYHEP YLCVFSENEV
1460 1470 1480 1490 1500
DIFNVTLAEW VQTINLRSAK PLSGDGILST CLCNDSPIFV LLQNVLQDQD
1510 1520 1530 1540 1550
SIEVPVNLAS GSTDGRKVTR RKFTFRTIGK DDRSASERRS HIQISTPSDF
1560 1570 1580 1590
MHIVHMGPAP VMELQQNFID LQSNHSHTSS DKDSLNRSVN ND
Length:1,592
Mass (Da):180,744
Last modified:October 1, 2002 - v3
Checksum:iF909FA8F5C9C876C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO081273 Genomic DNA Translation: CCD70382.1
PIRiT25808
RefSeqiNP_504599.2, NM_072198.5
UniGeneiCel.20082

Genome annotation databases

EnsemblMetazoaiK08B12.5a.1; K08B12.5a.1; WBGene00006437
K08B12.5a.2; K08B12.5a.2; WBGene00006437
GeneIDi179013
UCSCiK08B12.5.1 c. elegans

Similar proteinsi

Entry informationi

Entry nameiMRCK_CAEEL
AccessioniPrimary (citable) accession number: O01583
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 4, 2015
Last sequence update: October 1, 2002
Last modified: March 28, 2018
This is version 166 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome