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Protein

Serine/threonine-protein kinase smg-1

Gene

smg-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Serine/threonine protein kinase involved in mRNA surveillance. Recognizes the substrate consensus sequence [ST]-Q. Involved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by phosphorylating smg-2.2 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Mn2+By similarity

GO - Molecular functioni

GO - Biological processi

  • hermaphrodite genitalia development Source: WormBase
  • male genitalia development Source: WormBase
  • nuclear-transcribed mRNA catabolic process, nonsense-mediated decay Source: WormBase
  • phosphatidylinositol-3-phosphate biosynthetic process Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Nonsense-mediated mRNA decay

Keywords - Ligandi

ATP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-CEL-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
SignaLinkiO01510.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase smg-1 (EC:2.7.11.1)
Alternative name(s):
Suppressor with morphogenetic effect on genitalia protein 1
Gene namesi
Name:smg-1
ORF Names:C48B6.6
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome I

Organism-specific databases

WormBaseiC48B6.6a; CE30258; WBGene00004879; smg-1.
C48B6.6b; CE36919; WBGene00004879; smg-1.

Subcellular locationi

  • Cytoplasm 1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi1966 – 19661D → A: Loss of function. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 23222322Serine/threonine-protein kinase smg-1PRO_0000229794Add
BLAST

Proteomic databases

EPDiO01510.
PaxDbiO01510.
PRIDEiO01510.

Interactioni

Subunit structurei

Component of a post-splicing multiprotein NMD complex.

Protein-protein interaction databases

STRINGi6239.C48B6.6a.

Structurei

3D structure databases

ProteinModelPortaliO01510.
SMRiO01510. Positions 1932-2107.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini872 – 1535664FATPROSITE-ProRule annotationAdd
BLAST
Repeati1485 – 152137HEATAdd
BLAST
Domaini1780 – 2110331PI3K/PI4KPROSITE-ProRule annotationAdd
BLAST
Domaini2290 – 232233FATCPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the PI3/PI4-kinase family.Curated
Contains 1 FAT domain.PROSITE-ProRule annotation
Contains 1 FATC domain.PROSITE-ProRule annotation
Contains 1 HEAT repeat.Curated
Contains 1 PI3K/PI4K domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0891. Eukaryota.
COG5032. LUCA.
GeneTreeiENSGT00830000128322.
HOGENOMiHOG000154345.
InParanoidiO01510.
KOiK08873.
OMAiDWSARER.
OrthoDBiEOG7RV9F7.
PhylomeDBiO01510.

Family and domain databases

Gene3Di1.10.1070.11. 3 hits.
1.25.10.10. 2 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR003152. FATC_dom.
IPR011009. Kinase-like_dom.
IPR000403. PI3/4_kinase_cat_dom.
IPR014009. PIK_FAT.
IPR031559. SMG1.
[Graphical view]
PfamiPF02260. FATC. 1 hit.
PF00454. PI3_PI4_kinase. 1 hit.
PF15785. SMG1. 2 hits.
[Graphical view]
SMARTiSM01343. FATC. 1 hit.
SM00146. PI3Kc. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 7 hits.
SSF56112. SSF56112. 2 hits.
PROSITEiPS51189. FAT. 1 hit.
PS51190. FATC. 1 hit.
PS50290. PI3_4_KINASE_3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform a (identifier: O01510-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MITSRNNDIG NLIEQFRQRD TPQKERKAIL ARIEEILQTT KNVESLCVKW
60 70 80 90 100
TYLLDNLCWP SLTKHDRNDM KTLAGKVIRL VGVLLFDTES YPEFLIYLGT
110 120 130 140 150
LYQSVTKKSE ETRADIVFSV YFIVGVISQK TENRLIATDT ENVEKSLDWI
160 170 180 190 200
IKVLPNSSIS VYNHCLKGFV LVANTFPNVY AAMFESTLRA ILTNLPDFNS
210 220 230 240 250
HEKNFELLID TVMRFSDQLN EKPHLAEEMV RIIRPDIKKN GLGNMRELKK
260 270 280 290 300
RMKLTMALVK MAKSQKMLEE TNQMISEMSI ELEENGGKWS SASLITIVCD
310 320 330 340 350
VFNELLILGK DDVKLQKGVE ESLCNVLKDL NLSNQSTMEK QAFFNSLAKI
360 370 380 390 400
VKQLPAESQV KTRVHQIVFN TETGLFTPKN RDNRMFGHNM IYKDLINLVS
410 420 430 440 450
VLLTPTSLNH LQATYTDLRK IMIDSMSRLK QSEDTPYSDN IRWNESILLL
460 470 480 490 500
FFSSLQSISC AKSSLIVMMG IRPSIFEFFS SELPLTEYWL ASNHPEVYHL
510 520 530 540 550
FITIFVGHLK AHDFYIVQSD YIVRGDSIGQ SIGQTKRDYA RKQVVALQKI
560 570 580 590 600
INNFGDKLWK KTRLMISSWL HSLIATACEH QIGSDSFSQR EWVRLRNTVI
610 620 630 640 650
HQSVLTWNNE CVNQALTILS TATKWSELTS DIHRDIADKT KKAKWKEATT
660 670 680 690 700
IWESGDCNTY IRQSMSTVYQ MSQERQQKTI TSTSFGAEEF IIITNFLLKQ
710 720 730 740 750
ATPTTFKKGQ NSWMDEVLET FTQGCRTLEK PDSYVPETFI EKWDWIINQT
760 770 780 790 800
ANFCIVNKMK TPLGKPMQTF AAFENEIKRL AKEVIVRKNS DKKLNKSSTE
810 820 830 840 850
DPNQSPPLKY SVQWLRVHLL LKLIVVLEKL MNSAIHGGSS VFNLTEIPVS
860 870 880 890 900
SRQFFTVNAA SCEVWLNRVY YPALLVAYFN GYYGLVIRFG SNALSHFARQ
910 920 930 940 950
KDGDNDKKIV NGVCTASLMS LSMAVLGEPM EIVGLRRKVR EEFGTDMGQS
960 970 980 990 1000
LMEALGEMAN ARYETALVAL EAVLVTDAAT NETLKMIIQL AMVDILNRIR
1010 1020 1030 1040 1050
LPQATDYYKV VLFGEESNDS TITEDFRSVE LLTKFEKLNN TVNEKRQVVD
1060 1070 1080 1090 1100
WSARERFQFV ESAFSQTMRR TELLDIQKDF SAMGALALSA DSSCKLYSDI
1110 1120 1130 1140 1150
SSTSLIIANL VNKMTGVSQW KNKLTDTEIF DRNEEGNDGD KLAICRKLMH
1160 1170 1180 1190 1200
WGRHTKSNRG QSCAAHSEII RLSRKTSNCE LAFFHINSAI RGEKLAAWQR
1210 1220 1230 1240 1250
LEVERQRLKL VKTQNLDVRI REMNEVFGSL AEVFTTSCQL KSDFQMVDGM
1260 1270 1280 1290 1300
IKEKMISEGY NEDIAKREEH MSRASIQLAD FFQSLPELEN VLAPNLFPTI
1310 1320 1330 1340 1350
IWSELQRRSD SLSAGYHGIV GSLFHLASEM CPSLAKAHLK MARWAYEIAK
1360 1370 1380 1390 1400
IKNFPAENLS FYKFGKDETE NEELLKSLEA TSLVNLEKLV RAAISDDLRA
1410 1420 1430 1440 1450
NNILAPNSHF MHIWKMVRDH RTKFLSIAVT SYFQFIQNMS GDCDNLPYSK
1460 1470 1480 1490 1500
KEETTLATLR ILELLVKHGD VLIDVINDGL NKTNVHIWKE ILPQLFARLS
1510 1520 1530 1540 1550
HPSEHIRKTL VDLISKICTA APHAVVFQVV SGAASSSTDG EELEEQQNDD
1560 1570 1580 1590 1600
RNRVRACCEK LETNMSQSYP NLVKDVRQFV AELERINLLN EEKWSVVMGT
1610 1620 1630 1640 1650
MEHEMEKRLS LIRTENAKTE SALHLTASVK NDIIVKRTQL LTRQIFDVLD
1660 1670 1680 1690 1700
ELYQQTVIEP PKSKNEEEFV TAFAEVLTNA FQESRISRTT SPEKSWIPFK
1710 1720 1730 1740 1750
NLIANFVHRN SKKGMQTFET EDISPYLASL SNSCVPMPGQ ESVEFDRVVS
1760 1770 1780 1790 1800
ISRVARQVTI LPTKTRPKKL GFVGSDGKQV AFLFKGREDL HLDERVMQFL
1810 1820 1830 1840 1850
RLCNVMLQPG KGKHRQSVAA YQAHHYAVIP LGPRSGLIKW VEGATPMFHI
1860 1870 1880 1890 1900
YRKWQMKEKA LKQATKKNGE TVPEIERPSN MYHNMIRLAF ADHKIDSSIT
1910 1920 1930 1940 1950
SDRSKWPAEI LEEVFESLTA KTPTDLISRE LWMRANDATT WWSVTKRYSR
1960 1970 1980 1990 2000
SLAVMSMVGS VLGLGDRHLD NLLVDLKWGH VVHIDYNICF DKGKNLRIPE
2010 2020 2030 2040 2050
TVPFRLTRNM RHALGPSEMY GTFRESCVHV LSTLRSGHQV LTMLLDAFVF
2060 2070 2080 2090 2100
DPLVDWTSHE HTATSGVSLA LQLAVYGSNW KTKAKERLTD AMELLNLRMS
2110 2120 2130 2140 2150
EVQTLWLANR DDLLHWMKQV TECLLIENSM LGANAIYAQQ RVKAGTELRE
2160 2170 2180 2190 2200
AVTRHHALAK ELRPLIRVIG KEREEFADYL KFYKQALFDP LLKGHSALRN
2210 2220 2230 2240 2250
ELDIDTCVYN FNIVMQNIDN VFGALVNLSF TPIETITSRT SQQEFKPPPG
2260 2270 2280 2290 2300
LENVWVVKQD QQENSQAREV VRRVERRLNG WLDGSAGPDR KLSPREEADI
2310 2320
LIAEATSTPN LSQMYEGWTA WV
Length:2,322
Mass (Da):265,479
Last modified:June 1, 2002 - v3
Checksum:iB97E86C770C3356D
GO
Isoform b (identifier: O01510-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1857-2159: Missing.

Note: No experimental confirmation available.
Show »
Length:2,019
Mass (Da):230,928
Checksum:iC3C7EF3D547DEECA
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1857 – 2159303Missing in isoform b. CuratedVSP_017753Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF149821 mRNA. Translation: AAD48773.1.
FO080896 Genomic DNA. Translation: CCD67583.1.
FO080896 Genomic DNA. Translation: CCD67584.1.
PIRiT28924.
RefSeqiNP_001021053.1. NM_001025882.2. [O01510-1]
NP_001021054.1. NM_001025883.3. [O01510-2]
UniGeneiCel.19562.

Genome annotation databases

EnsemblMetazoaiC48B6.6a; C48B6.6a; WBGene00004879. [O01510-1]
GeneIDi172418.
KEGGicel:CELE_C48B6.6.
UCSCiC48B6.6a. c. elegans. [O01510-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF149821 mRNA. Translation: AAD48773.1.
FO080896 Genomic DNA. Translation: CCD67583.1.
FO080896 Genomic DNA. Translation: CCD67584.1.
PIRiT28924.
RefSeqiNP_001021053.1. NM_001025882.2. [O01510-1]
NP_001021054.1. NM_001025883.3. [O01510-2]
UniGeneiCel.19562.

3D structure databases

ProteinModelPortaliO01510.
SMRiO01510. Positions 1932-2107.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi6239.C48B6.6a.

Proteomic databases

EPDiO01510.
PaxDbiO01510.
PRIDEiO01510.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiC48B6.6a; C48B6.6a; WBGene00004879. [O01510-1]
GeneIDi172418.
KEGGicel:CELE_C48B6.6.
UCSCiC48B6.6a. c. elegans. [O01510-1]

Organism-specific databases

CTDi172418.
WormBaseiC48B6.6a; CE30258; WBGene00004879; smg-1.
C48B6.6b; CE36919; WBGene00004879; smg-1.

Phylogenomic databases

eggNOGiKOG0891. Eukaryota.
COG5032. LUCA.
GeneTreeiENSGT00830000128322.
HOGENOMiHOG000154345.
InParanoidiO01510.
KOiK08873.
OMAiDWSARER.
OrthoDBiEOG7RV9F7.
PhylomeDBiO01510.

Enzyme and pathway databases

ReactomeiR-CEL-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
SignaLinkiO01510.

Miscellaneous databases

NextBioi875429.
PROiO01510.

Family and domain databases

Gene3Di1.10.1070.11. 3 hits.
1.25.10.10. 2 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR003152. FATC_dom.
IPR011009. Kinase-like_dom.
IPR000403. PI3/4_kinase_cat_dom.
IPR014009. PIK_FAT.
IPR031559. SMG1.
[Graphical view]
PfamiPF02260. FATC. 1 hit.
PF00454. PI3_PI4_kinase. 1 hit.
PF15785. SMG1. 2 hits.
[Graphical view]
SMARTiSM01343. FATC. 1 hit.
SM00146. PI3Kc. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 7 hits.
SSF56112. SSF56112. 2 hits.
PROSITEiPS51189. FAT. 1 hit.
PS51190. FATC. 1 hit.
PS50290. PI3_4_KINASE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "SMG-1 is a phosphatidylinositol kinase-related protein kinase required for nonsense-mediated mRNA Decay in Caenorhabditis elegans."
    Grimson A., O'Connor S., Newman C.L., Anderson P.
    Mol. Cell. Biol. 24:7483-7490(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF ASP-1966.
    Strain: Bristol N2.
  2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
    Strain: Bristol N2.
  3. "A new kind of informational suppression in the nematode Caenorhabditis elegans."
    Hodgkin J., Papp A., Pulak R., Ambros V., Anderson P.
    Genetics 123:301-313(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiSMG1_CAEEL
AccessioniPrimary (citable) accession number: O01510
Secondary accession number(s): Q6BEW0, Q9U9U7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: June 1, 2002
Last modified: May 11, 2016
This is version 129 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.