Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

G1/S-specific cyclin-E

Gene

cye-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Essential for the control of the cell cycle at the G1/S (start) transition (PubMed:12606285). In association with cdk-2, regulates proliferation, quiescent state and cell fate during the development of several cell lineages (PubMed:10952902, PubMed:17476329, PubMed:21558371, PubMed:21455289). In the embryo, initiates the establishment of cell polarity through the recruitment of the centrosomal proteins spd-2 and spd-5 during prophase (PubMed:17115027). During the development of the vulva, controls the onset of vulval cell terminal differentiation by controlling the duration of G1 phase (PubMed:10952902, PubMed:20005870). During hypoderm development at early larval stages, controls syncytial fate of seam cell daughter cells (PubMed:17476329). By controlling the activity of translational repressor gld-1, regulates the pool of germline stem cells and the size of the mitotic zone by preventing entry into meiosis (PubMed:21455289). In addition, repression of expression by gld-1 prevents mitosis re-entry in meiotic germline cells (PubMed:19758560).8 Publications

GO - Molecular functioni

  • cyclin-dependent protein serine/threonine kinase regulator activity Source: UniProtKB

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • DNA endoreduplication Source: WormBase
  • embryo development ending in birth or egg hatching Source: WormBase
  • G1/S transition of mitotic cell cycle Source: UniProtKB
  • germ cell development Source: WormBase
  • germline cell cycle switching, mitotic to meiotic cell cycle Source: WormBase
  • gonad development Source: UniProtKB
  • hermaphrodite genitalia morphogenesis Source: UniProtKB
  • meiotic cell cycle Source: UniProtKB-KW
  • mitotic cell cycle arrest Source: WormBase
  • mitotic nuclear division Source: UniProtKB-KW
  • negative regulation of cell division Source: UniProtKB
  • positive regulation of cell proliferation Source: WormBase
  • positive regulation of protein localization to centrosome Source: UniProtKB
  • positive regulation of vulval development Source: WormBase
  • post-embryonic development Source: WormBase
  • posttranscriptional regulation of gene expression Source: WormBase
  • protein phosphorylation Source: WormBase
  • regulation of meiotic cell cycle Source: UniProtKB
  • regulation of mitotic nuclear division Source: UniProtKB
  • regulation of protein kinase activity Source: GOC
  • regulation of protein localization to cell cortex Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Cyclin

Keywords - Biological processi

Cell cycle, Cell division, Meiosis, Mitosis

Enzyme and pathway databases

ReactomeiR-CEL-113510. E2F mediated regulation of DNA replication.
R-CEL-69205. G1/S-Specific Transcription.

Names & Taxonomyi

Protein namesi
Recommended name:
G1/S-specific cyclin-E
Gene namesi
Name:cye-1
ORF Names:C37A2.4
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome I

Organism-specific databases

WormBaseiC37A2.4a; CE24832; WBGene00000871; cye-1.
C37A2.4b; CE33761; WBGene00000871; cye-1.

Subcellular locationi

GO - Cellular componenti

  • centriole Source: UniProtKB-SubCell
  • chromatin Source: UniProtKB
  • cyclin-dependent protein kinase holoenzyme complex Source: WormBase
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

Pathology & Biotechi

Disruption phenotypei

Loss of cell division in vulval lineages as a result of lengthened intervals between cell divisons (PubMed:20005870). RNAi-mediated knockdown results in arrest prior to the 100-cell embryonic stage (PubMed:10952902). Depending on the knockdown, some animals reach adult age (PubMed:17476329). In the 1-cell embryo, persistent ruffling throughout the embryo cortex and mislocalization of par-2, which remains cytoplasmic, and par-6, which is distributed throughout the cortex. In 50 percent of embryos, the first division is symmetric. Adult mutants are sterile due to a lack of sperm and egg production (PubMed:17115027). In mutants and knockdown animals, production of 2 additional distal tip cells (DTC) is often associated with the production of an extra gonad (PubMed:17476329). In addition, gonads of knockdown animals have an abnormal mitotic zone characterized by an enlargement of the distal germ cell nuclei, a reduction in the number of mitotic germ cells, a reduction in the mitotic region length and an abnormal expression of gld-1 (PubMed:21455289).5 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 524524G1/S-specific cyclin-EPRO_0000080458Add
BLAST

Proteomic databases

EPDiO01501.
PaxDbiO01501.
PRIDEiO01501.

Expressioni

Tissue specificityi

Expressed dynamically in proliferating cells throughout development. Detectable in larval blast cells undergoing active proliferation that give rise to all tissue types, including germline, intestine, hypodermis, neurons, and muscle.1 Publication

Developmental stagei

Expressed both maternally and zygotically. Ubiquitous embryonic pattern of expression declines during embryogenesis and disappears from most cells in comma-stage embryos coincident with the completion of the majority of embryonic cell divisions. Expression levels drop and become restricted and dynamic during postembryonic development (PubMed:12606285). During the development of distal tip cells, expressed asymmetrically between the daughters of the Z1.a and Z4.p cells; asymmetric expression is regulated by wrm-1, a component of the Wnt/MAPK pathway (PubMed:17476329). In the gonads, expression is restricted to the proliferating distal germline cells (PubMed:12606285, PubMed:21558371). In germline cells entering meiosis, expression is repressed by gld-1 (PubMed:21455289, PubMed:19758560).5 Publications

Interactioni

Subunit structurei

Interacts with a member of the CDK2/CDK protein kinases to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex (By similarity).By similarity

Protein-protein interaction databases

BioGridi37847. 16 interactions.
IntActiO01501. 6 interactions.
STRINGi6239.C37A2.4a.

Structurei

3D structure databases

ProteinModelPortaliO01501.
SMRiO01501. Positions 232-504.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cyclin family. Cyclin E subfamily.Curated

Phylogenomic databases

eggNOGiKOG0655. Eukaryota.
ENOG410XS2J. LUCA.
GeneTreeiENSGT00760000118939.
HOGENOMiHOG000111449.
InParanoidiO01501.
KOiK06626.
OMAiSEESHEM.
OrthoDBiEOG7HB595.
PhylomeDBiO01501.

Family and domain databases

Gene3Di1.10.472.10. 2 hits.
InterProiIPR013763. Cyclin-like.
IPR006671. Cyclin_N.
[Graphical view]
PfamiPF00134. Cyclin_N. 1 hit.
[Graphical view]
SMARTiSM00385. CYCLIN. 1 hit.
[Graphical view]
SUPFAMiSSF47954. SSF47954. 2 hits.
PROSITEiPS00292. CYCLINS. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform a1 Publication (identifier: O01501-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAGRKSSRTA ERVPTTQKPE RKSAILSPHD ELRERLLETA IDMKENIPQR
60 70 80 90 100
NTRNSSVGSQ KSDCSETRKR RSTKEGPAAK RHSGEKHRNG SREDSLEYIS
110 120 130 140 150
EYSDDREVGS SSSQSSRTRG QPLPAMPEEE EVFDKSSSSD NLAESEESHE
160 170 180 190 200
MVRLEERQDI EEEIEDDFDD EEEDVVNDKE EYEEIESEDE DDYPVQNEGF
210 220 230 240 250
AVTKRLMNDE HMVTAPTFLS TAKCDGIGSP TKVWSLMVKR DEIPRATRFL
260 270 280 290 300
LGNHPDMDDE KRRILIDWMM EVCESEKLHR ETFHLAVDYV DRYLESSNVE
310 320 330 340 350
CSTDNFQLVG TAALFIAAKY EEIYPPKCID FAHLTDSAFT CDNIRTMEVL
360 370 380 390 400
IVKYIGWSLG PITSIQWLST YLQLLGTGKK NKSDHYEEQN MYVPELLRSE
410 420 430 440 450
YLEMCKILDF LLFEIDSFTF SYRTIAAAVL FVNYEPTCAV EKATGFMQAQ
460 470 480 490 500
LEKVIEYVEP VCRAFAKQRQ LLDDVIPKHE SIKSDDSHNI QVYVKRSSME
510 520
PIVKSERERI QHLKARRLHP QRLF
Length:524
Mass (Da):60,586
Last modified:October 1, 2001 - v2
Checksum:i5B16BA7E70AA5F15
GO
Isoform b1 Publication (identifier: O01501-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     100-102: Missing.

Show »
Length:521
Mass (Da):60,206
Checksum:i90CCAFAB44064ECC
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei100 – 1023Missing in isoform b. 1 PublicationVSP_007909

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF520616 mRNA. Translation: AAM78547.1.
AF058331 mRNA. Translation: AAC63505.1.
FO080805 Genomic DNA. Translation: CCD66921.1.
FO080805 Genomic DNA. Translation: CCD66922.1.
PIRiT43050.
RefSeqiNP_001021027.1. NM_001025856.3. [O01501-1]
NP_001021028.1. NM_001025857.2. [O01501-2]
UniGeneiCel.9296.

Genome annotation databases

EnsemblMetazoaiC37A2.4a; C37A2.4a; WBGene00000871. [O01501-1]
GeneIDi172399.
KEGGicel:CELE_C37A2.4.
UCSCiC37A2.4a. c. elegans. [O01501-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF520616 mRNA. Translation: AAM78547.1.
AF058331 mRNA. Translation: AAC63505.1.
FO080805 Genomic DNA. Translation: CCD66921.1.
FO080805 Genomic DNA. Translation: CCD66922.1.
PIRiT43050.
RefSeqiNP_001021027.1. NM_001025856.3. [O01501-1]
NP_001021028.1. NM_001025857.2. [O01501-2]
UniGeneiCel.9296.

3D structure databases

ProteinModelPortaliO01501.
SMRiO01501. Positions 232-504.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi37847. 16 interactions.
IntActiO01501. 6 interactions.
STRINGi6239.C37A2.4a.

Proteomic databases

EPDiO01501.
PaxDbiO01501.
PRIDEiO01501.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiC37A2.4a; C37A2.4a; WBGene00000871. [O01501-1]
GeneIDi172399.
KEGGicel:CELE_C37A2.4.
UCSCiC37A2.4a. c. elegans. [O01501-1]

Organism-specific databases

CTDi172399.
WormBaseiC37A2.4a; CE24832; WBGene00000871; cye-1.
C37A2.4b; CE33761; WBGene00000871; cye-1.

Phylogenomic databases

eggNOGiKOG0655. Eukaryota.
ENOG410XS2J. LUCA.
GeneTreeiENSGT00760000118939.
HOGENOMiHOG000111449.
InParanoidiO01501.
KOiK06626.
OMAiSEESHEM.
OrthoDBiEOG7HB595.
PhylomeDBiO01501.

Enzyme and pathway databases

ReactomeiR-CEL-113510. E2F mediated regulation of DNA replication.
R-CEL-69205. G1/S-Specific Transcription.

Miscellaneous databases

PROiO01501.

Family and domain databases

Gene3Di1.10.472.10. 2 hits.
InterProiIPR013763. Cyclin-like.
IPR006671. Cyclin_N.
[Graphical view]
PfamiPF00134. Cyclin_N. 1 hit.
[Graphical view]
SMARTiSM00385. CYCLIN. 1 hit.
[Graphical view]
SUPFAMiSSF47954. SSF47954. 2 hits.
PROSITEiPS00292. CYCLINS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cyclin E expression during development in Caenorhabditis elegans."
    Brodigan T.M., Liu J., Park M., Kipreos E.T., Krause M.
    Dev. Biol. 254:102-115(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A AND B), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
  2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
    Strain: Bristol N2.
  3. "Mutations in cye-1, a Caenorhabditis elegans cyclin E homolog, reveal coordination between cell-cycle control and vulval development."
    Fay D.S., Han M.
    Development 127:4049-4060(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  4. "Cyclin E-Cdk2 temporally regulates centrosome assembly and establishment of polarity in Caenorhabditis elegans embryos."
    Cowan C.R., Hyman A.A.
    Nat. Cell Biol. 8:1441-1447(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
  5. "Cyclin E and CDK2 repress the terminal differentiation of quiescent cells after asymmetric division in C. elegans."
    Fujita M., Takeshita H., Sawa H.
    PLoS ONE 2:E407-E407(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.
  6. "Translational repression of cyclin E prevents precocious mitosis and embryonic gene activation during C. elegans meiosis."
    Biedermann B., Wright J., Senften M., Kalchhauser I., Sarathy G., Lee M.H., Ciosk R.
    Dev. Cell 17:355-364(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DEVELOPMENTAL STAGE.
  7. "C. elegans BED domain transcription factor BED-3 controls lineage-specific cell proliferation during organogenesis."
    Inoue T., Sternberg P.W.
    Dev. Biol. 338:226-236(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  8. "Cyclin E and CDK-2 regulate proliferative cell fate and cell cycle progression in the C. elegans germline."
    Fox P.M., Vought V.E., Hanazawa M., Lee M.H., Maine E.M., Schedl T.
    Development 138:2223-2234(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DEVELOPMENTAL STAGE.
  9. "Cyclin E and Cdk2 control GLD-1, the mitosis/meiosis decision, and germline stem cells in Caenorhabditis elegans."
    Jeong J., Verheyden J.M., Kimble J.
    PLoS Genet. 7:E1001348-E1001348(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiCCNE_CAEEL
AccessioniPrimary (citable) accession number: O01501
Secondary accession number(s): O77098, Q86FL2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2003
Last sequence update: October 1, 2001
Last modified: June 8, 2016
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.