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Protein

Signal transducing adapter molecule 1

Gene

stam-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds, sorts and targets the polycystin complex (lov-1 and pkd-2) for lysosomal degradation, acting on early endosomes located at the ciliary base. Functions in the germline together with the ephrin receptor (vab-1) signaling pathway to negatively regulate MAPK activation. May have a role as a positive regulator of meiotic maturation in oocytes, acting independently of vab-1.2 Publications

GO - Molecular functioni

  • protein C-terminus binding Source: WormBase
  • ubiquitin binding Source: WormBase

GO - Biological processi

  • endosome to lysosome transport Source: WormBase
  • ephrin receptor signaling pathway Source: UniProtKB
  • intracellular protein transport Source: InterPro
  • multicellular organism development Source: UniProtKB-KW
  • negative regulation of MAPK cascade Source: UniProtKB
  • protein localization to nonmotile primary cilium Source: WormBase
  • response to hermaphrodite contact Source: WormBase
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-CEL-182971. EGFR downregulation.
R-CEL-917729. Endosomal Sorting Complex Required For Transport (ESCRT).
SignaLinkiO01498.

Names & Taxonomyi

Protein namesi
Recommended name:
Signal transducing adapter molecule 1By similarity
Short name:
STAM-1By similarity
Alternative name(s):
Prion-like-(Q/N-rich) domain-bearing protein 19
Gene namesi
Name:stam-1
Synonyms:pqn-19
ORF Names:C34G6.7
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome I

Organism-specific databases

WormBaseiC34G6.7a; CE29700; WBGene00004109; stam-1.
C34G6.7b; CE32816; WBGene00004109; stam-1.

Subcellular locationi

  • Cell projectioncilium 1 Publication
  • Endosome 1 Publication

  • Note: Located to early endosomes at the ciliary base but not cilium proper of male-specific sensory neurons.1 Publication

GO - Cellular componenti

  • cilium Source: UniProtKB
  • early endosome Source: WormBase
  • endosome Source: UniProtKB
  • ESCRT-0 complex Source: WormBase
Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cilium, Endosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 457457Signal transducing adapter molecule 1PRO_0000347284Add
BLAST

Proteomic databases

EPDiO01498.
PaxDbiO01498.
PRIDEiO01498.

Expressioni

Tissue specificityi

Widely expressed, including the pharyngeal intestinal valve, several head neurons, and phasmids in both males and hermaphrodites throughout development. In males, also expressed in the gonad and sensory neurons in the tail. Expressed in the male-specific ciliate CEM, ray B-type and hook HOB sensory neurons.1 Publication

Interactioni

Subunit structurei

Isoform a, but not isoform b, interacts (via C-terminus) with lov-1 (via C-terminus).1 Publication

GO - Molecular functioni

  • protein C-terminus binding Source: WormBase
  • ubiquitin binding Source: WormBase

Protein-protein interaction databases

BioGridi37718. 8 interactions.
DIPiDIP-26020N.
IntActiO01498. 15 interactions.
MINTiMINT-1124360.
STRINGi6239.C34G6.7a.2.

Structurei

3D structure databases

ProteinModelPortaliO01498.
SMRiO01498. Positions 7-146, 222-367.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini19 – 147129VHSPROSITE-ProRule annotationAdd
BLAST
Domaini173 – 19220UIMPROSITE-ProRule annotationAdd
BLAST
Domaini219 – 27860SH3PROSITE-ProRule annotationAdd
BLAST
Domaini366 – 38318ITAMSequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi372 – 45685Gln-richSequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the STAM family.Sequence analysis
Contains 1 ITAM domain.Sequence analysis
Contains 1 SH3 domain.PROSITE-ProRule annotation
Contains 1 UIM (ubiquitin-interacting motif) domain.PROSITE-ProRule annotation
Contains 1 VHS domain.PROSITE-ProRule annotation

Keywords - Domaini

SH3 domain

Phylogenomic databases

eggNOGiKOG2199. Eukaryota.
ENOG410XTSY. LUCA.
GeneTreeiENSGT00700000104139.
HOGENOMiHOG000017280.
InParanoidiO01498.
KOiK04705.
OMAiWANCEER.
PhylomeDBiO01498.

Family and domain databases

Gene3Di1.25.40.90. 1 hit.
InterProiIPR008942. ENTH_VHS.
IPR001452. SH3_domain.
IPR003903. UIM_dom.
IPR002014. VHS_dom.
[Graphical view]
PfamiPF00018. SH3_1. 1 hit.
PF00790. VHS. 1 hit.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
SMARTiSM00326. SH3. 1 hit.
SM00288. VHS. 1 hit.
[Graphical view]
SUPFAMiSSF48464. SSF48464. 1 hit.
SSF50044. SSF50044. 1 hit.
PROSITEiPS50002. SH3. 1 hit.
PS50330. UIM. 1 hit.
PS50179. VHS. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform a1 Publication (identifier: O01498-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKKQKSFPMS AYEDLLGKIT APTITVENWE GILAFCDMIN NDFEGSKTGI
60 70 80 90 100
KSLRKRLNNR DPHVVLLAIS VLDSCWANCE ERFRKEVSSA QFINELKALC
110 120 130 140 150
TSSQRQVAEK MRLTVQKWVD TECKTEQSLS LIVTLHKNLV ADGYSFVVDD
160 170 180 190 200
PKSKTKAIDA KFANDPNYVG SAQEEEAIAK AIAASLADAE KQEKAKKSTS
210 220 230 240 250
TMYPSAKASS PAVQTNSNIP EKNVRALYDF EAAESNELSF VAGDIITITD
260 270 280 290 300
ESNPHWWTGR IGTQQGLFPS SFVTNQLDDL KSKETDSSQK APEVVASINE
310 320 330 340 350
AILVRCLQVL HECDPTGERQ DPEDLAQLEA ASYAQGNLID AHLASIDRQS
360 370 380 390 400
NSLAQIDVAI RDVLALYDDA IQKGGFQHQS QGMYQQPMQQ YNYQQPPNRA
410 420 430 440 450
YYPPTGPVQQ QQPQQYPPQH YPAPGAQPQY ACPPNSVPQP QQQQQQWPAP

SSQPQQY
Note: No experimental confirmation available.Curated
Length:457
Mass (Da):50,830
Last modified:December 1, 2001 - v2
Checksum:i1AD9B1D83EBE2E16
GO
Isoform b1 Publication (identifier: O01498-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-8: Missing.
     397-405: PNRAYYPPT → VSFYSEKNI
     406-457: Missing.

Note: No experimental confirmation available.Curated
Show »
Length:397
Mass (Da):44,017
Checksum:i5CFD4FE172B63E75
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 88Missing in isoform b. 1 PublicationVSP_052896
Alternative sequencei397 – 4059PNRAYYPPT → VSFYSEKNI in isoform b. 1 PublicationVSP_052897
Alternative sequencei406 – 45752Missing in isoform b. 1 PublicationVSP_052898Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO080778 Genomic DNA. Translation: CCD66687.1.
FO080778 Genomic DNA. Translation: CCD66688.1.
PIRiG87789.
RefSeqiNP_491710.2. NM_059309.6. [O01498-1]
NP_871813.1. NM_182013.6. [O01498-2]
UniGeneiCel.7418.

Genome annotation databases

EnsemblMetazoaiC34G6.7a; C34G6.7a; WBGene00004109. [O01498-1]
GeneIDi172264.
KEGGicel:CELE_C34G6.7.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO080778 Genomic DNA. Translation: CCD66687.1.
FO080778 Genomic DNA. Translation: CCD66688.1.
PIRiG87789.
RefSeqiNP_491710.2. NM_059309.6. [O01498-1]
NP_871813.1. NM_182013.6. [O01498-2]
UniGeneiCel.7418.

3D structure databases

ProteinModelPortaliO01498.
SMRiO01498. Positions 7-146, 222-367.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi37718. 8 interactions.
DIPiDIP-26020N.
IntActiO01498. 15 interactions.
MINTiMINT-1124360.
STRINGi6239.C34G6.7a.2.

Proteomic databases

EPDiO01498.
PaxDbiO01498.
PRIDEiO01498.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiC34G6.7a; C34G6.7a; WBGene00004109. [O01498-1]
GeneIDi172264.
KEGGicel:CELE_C34G6.7.

Organism-specific databases

CTDi172264.
WormBaseiC34G6.7a; CE29700; WBGene00004109; stam-1.
C34G6.7b; CE32816; WBGene00004109; stam-1.

Phylogenomic databases

eggNOGiKOG2199. Eukaryota.
ENOG410XTSY. LUCA.
GeneTreeiENSGT00700000104139.
HOGENOMiHOG000017280.
InParanoidiO01498.
KOiK04705.
OMAiWANCEER.
PhylomeDBiO01498.

Enzyme and pathway databases

ReactomeiR-CEL-182971. EGFR downregulation.
R-CEL-917729. Endosomal Sorting Complex Required For Transport (ESCRT).
SignaLinkiO01498.

Miscellaneous databases

NextBioi874735.
PROiO01498.

Family and domain databases

Gene3Di1.25.40.90. 1 hit.
InterProiIPR008942. ENTH_VHS.
IPR001452. SH3_domain.
IPR003903. UIM_dom.
IPR002014. VHS_dom.
[Graphical view]
PfamiPF00018. SH3_1. 1 hit.
PF00790. VHS. 1 hit.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
SMARTiSM00326. SH3. 1 hit.
SM00288. VHS. 1 hit.
[Graphical view]
SUPFAMiSSF48464. SSF48464. 1 hit.
SSF50044. SSF50044. 1 hit.
PROSITEiPS50002. SH3. 1 hit.
PS50330. UIM. 1 hit.
PS50179. VHS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
    Strain: Bristol N2.
  2. "Galphao/i and Galphas signaling function in parallel with the MSP/Eph receptor to control meiotic diapause in C. elegans."
    Govindan J.A., Cheng H., Harris J.E., Greenstein D.
    Curr. Biol. 16:1257-1268(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  3. "STAM and Hrs down-regulate ciliary TRP receptors."
    Hu J., Wittekind S.G., Barr M.M.
    Mol. Biol. Cell 18:3277-3289(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH LOV-1, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiSTAM1_CAEEL
AccessioniPrimary (citable) accession number: O01498
Secondary accession number(s): Q8I7I2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: December 1, 2001
Last modified: May 11, 2016
This is version 130 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.