O01427 (AIR2_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aurora/IPL1-related protein kinase 2
EC=2.7.11.1

Alternative name(s):
Serine/threonine-protein kinase aurora-B

Gene names

Name:	air-2
Synonyms:	stu-7
ORF Names:	B0207.4

Organism

Caenorhabditis elegans

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	305 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Serine/threonine-protein kinase which mediates both meiotic and mitotic chromosome segregation. Required for histone H3 'Ser-10' phosphorylation. Phosphorylates tlk-1 at 'Ser-634', which enhances its activity. Phosphorylates zen-4 at 'Ser-680'. Ref.1 Ref.3 Ref.4 Ref.5 Ref.6 Ref.7 Ref.8 Ref.9 Ref.11 Ref.12
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subunit structure	Interacts with zen-4 and icp-1. Part of a complex containing at least air-2; icp-1; csc-1 and bir-1. Interacts with tlk-1 and bmk-1. Ref.5 Ref.6 Ref.12 Ref.13
Subcellular location	Cytoplasm › cytoskeleton. Chromosome. Midbody. Note: Meiotic and mitotic chromosomes. During each division, relocates to the midbody microtubules. Ref.1
Developmental stage	Present during gametogenesis and throughout embryogenesis (at protein level). Ref.1
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Chromosome partition Meiosis Mitosis
Cellular component	Chromosome Cytoplasm Cytoskeleton
Ligand	ATP-binding Nucleotide-binding
Molecular function	Chromatin regulator Developmental protein Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	chromatin modification Inferred from electronic annotation. Source: UniProtKB-KW chromosome condensation Inferred from mutant phenotype Ref.7. Source: WormBase chromosome segregation Inferred from mutant phenotype Ref.7Ref.5. Source: WormBase cytokinesis, completion of separation Inferred from mutant phenotype Ref.7. Source: WormBase embryo development ending in birth or egg hatching Inferred from mutant phenotype Ref.5Ref.8. Source: WormBase establishment of protein localization Inferred from mutant phenotype Ref.5. Source: WormBase hermaphrodite genitalia development Inferred from mutant phenotype. Source: WormBase locomotion Inferred from mutant phenotype. Source: WormBase meiosis Inferred from mutant phenotype Ref.7Ref.9Ref.8. Source: WormBase peptidyl-serine phosphorylation Inferred from direct assay Ref.12. Source: WormBase peptidyl-tyrosine phosphorylation Inferred from direct assay. Source: WormBase protein autophosphorylation Inferred from direct assay Ref.12. Source: WormBase regulation of contractile ring contraction involved in cell cycle cytokinesis Inferred from mutant phenotype Ref.5. Source: WormBase spindle midzone assembly involved in mitosis Inferred from mutant phenotype Ref.5. Source: WormBase
Cellular component	condensed nuclear chromosome Inferred from direct assay Ref.7Ref.5. Source: WormBase cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW midbody Inferred from electronic annotation. Source: UniProtKB-SubCell spindle midzone Inferred from direct assay Ref.5. Source: WormBase
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATPase binding Inferred from physical interaction. Source: WormBase kinesin binding Inferred from physical interaction Ref.5. Source: WormBase protein serine/threonine kinase activity Inferred from direct assay Ref.9Ref.13Ref.12. Source: WormBase protein tyrosine kinase activity Inferred from direct assay. Source: WormBase
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
icp-1	Q9GSQ0	4	EBI-312947,EBI-312981
lin-10	O17583	2	EBI-312947,EBI-313389
tlk-1	P34314	3	EBI-312947,EBI-3890382

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 305	305	Aurora/IPL1-related protein kinase 2		PRO_0000268640
Regions
	Domain	30 – 280	251	Protein kinase
	Nucleotide binding	36 – 44	9	ATP By similarity
Sites
	Active site	153	1	Proton acceptor By similarity
	Binding site	59	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	O01427 [UniParc]. Last modified May 1, 2000. Version 2. Checksum: 1635EB60D2E14011	FASTA	305	34,749
	10 20 30 40 50 60 MENKPPVINL PEKETVNTPQ KGGKFTINDF EIGRPLGKGK FGSVYLARTK TGHFHVAIKV 70 80 90 100 110 120 LFKSQLISGG VEHQLEREIE IQSHLNHPNI IKLYTYFWDA KKIYLVLEYA PGGEMYKQLT 130 140 150 160 170 180 VSKRFSEPTA AKYMYEIADA LSYCHRKNVI HRDIKPENLL IGSQGELKIG DFGWSVHAPS 190 200 210 220 230 240 NKRQTMCGTM DYLPPEMVNG ADHSDAVDLW AIGVLCYEFL VGKPPFEHED QSKTYAAIKA 250 260 270 280 290 300 ARFTYPDSVK KGARDLIGRL LVVDPKARCT LEQVKEHYWI QGMMEAKIRA EKQQKIEKEA SLRNH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"AIR-2: an Aurora/Ipl1-related protein kinase associated with chromosomes and midbody microtubules is required for polar body extrusion and cytokinesis in Caenorhabditis elegans embryos." Schumacher J.M., Golden A., Donovan P.J. J. Cell Biol. 143:1635-1646(1998) [PubMed: 9852156] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE. Strain: Bristol N2.
[2]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
[3]	"Stu-7/air-2 is a C. elegans aurora homologue essential for chromosome segregation during embryonic and post-embryonic development." Woollard A., Hodgkin J. Mech. Dev. 82:95-108(1999) [PubMed: 10354474] [Abstract] Cited for: IDENTIFICATION, FUNCTION.
[4]	"Mitotic phosphorylation of histone H3 is governed by Ipl1/aurora kinase and Glc7/PP1 phosphatase in budding yeast and nematodes." Hsu J.-Y., Sun Z.-W., Li X., Reuben M., Tatchell K., Bishop D.K., Grushcow J.M., Brame C.J., Caldwell J.A., Hunt D.F., Lin R., Smith M.M., Allis C.D. Cell 102:279-291(2000) [PubMed: 10975519] [Abstract] Cited for: FUNCTION.
[5]	"The aurora-related kinase AIR-2 recruits ZEN-4/CeMKLP1 to the mitotic spindle at metaphase and is required for cytokinesis." Severson A.F., Hamill D.R., Carter J.C., Schumacher J., Bowerman B. Curr. Biol. 10:1162-1171(2000) [PubMed: 11050384] [Abstract] Cited for: FUNCTION, INTERACTION WITH ZEN-4.
[6]	"Incenp and an aurora-like kinase form a complex essential for chromosome segregation and efficient completion of cytokinesis." Kaitna S., Mendoza M., Jantsch-Plunger V., Glotzer M. Curr. Biol. 10:1172-1181(2000) [PubMed: 11050385] [Abstract] Cited for: FUNCTION, INTERACTION WITH ICP-1.
[7]	"The survivin-like C. elegans BIR-1 protein acts with the Aurora-like kinase AIR-2 to affect chromosomes and the spindle midzone." Speliotes E.K., Uren A., Vaux D., Horvitz H.R. Mol. Cell 6:211-223(2000) [PubMed: 10983970] [Abstract] Cited for: FUNCTION.
[8]	"The aurora B kinase AIR-2 regulates kinetochores during mitosis and is required for separation of homologous Chromosomes during meiosis." Kaitna S., Pasierbek P., Jantsch M., Loidl J., Glotzer M. Curr. Biol. 12:798-812(2002) [PubMed: 12015116] [Abstract] Cited for: FUNCTION.
[9]	"The aurora kinase AIR-2 functions in the release of chromosome cohesion in Caenorhabditis elegans meiosis." Rogers E., Bishop J.D., Waddle J.A., Schumacher J.M., Lin R. J. Cell Biol. 157:219-229(2002) [PubMed: 11940606] [Abstract] Cited for: FUNCTION.
[10]	"CSC-1: a subunit of the Aurora B kinase complex that binds to the survivin-like protein BIR-1 and the incenp-like protein ICP-1." Romano A., Guse A., Krascenicova I., Schnabel H., Schnabel R., Glotzer M. J. Cell Biol. 161:229-236(2003) [PubMed: 12707312] [Abstract] Cited for: IDENTIFICATION IN A COMPLEX WITH ICP-1; CSC-1 AND BIR-1.
[11]	"Phosphorylation of ZEN-4/MKLP1 by aurora B regulates completion of cytokinesis." Guse A., Mishima M., Glotzer M. Curr. Biol. 15:778-786(2005) [PubMed: 15854913] [Abstract] Cited for: FUNCTION.
[12]	"The C. elegans Tousled-like kinase contributes to chromosome segregation as a substrate and regulator of the Aurora B kinase." Han Z., Riefler G.M., Saam J.R., Mango S.E., Schumacher J.M. Curr. Biol. 15:894-904(2005) [PubMed: 15916946] [Abstract] Cited for: FUNCTION, INTERACTION WITH TLK-1.
[13]	"The Caenorhabditis elegans Aurora B kinase AIR-2 phosphorylates and is required for the localization of a BimC kinesin to meiotic and mitotic spindles." Bishop J.D., Han Z., Schumacher J.M. Mol. Biol. Cell 16:742-756(2005) [PubMed: 15548597] [Abstract] Cited for: INTERACTION WITH BMK-1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF071207 mRNA. Translation: AAC70945.1. FO080115 Genomic DNA. Translation: CCD61317.1.
PIR	B87790. T43221.
RefSeq	NP_491714.1. NM_059313.4.
UniGene	Cel.6613.
3D structure databases
HSSP	HSSP built from PDB template 1OL6 based on UniProtKB O14965.
ProteinModelPortal	O01427.
SMR	O01427. Positions 24-280.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-25477N.
IntAct	O01427. 9 interactions.
MINT	MINT-229556.
STRING	O01427.
Proteomic databases
PRIDE	O01427.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	B0207.4; B0207.4; B0207.4.
GeneID	172268.
KEGG	cel:B0207.4.
NMPDR	fig|6239.3.peg.1382.
UCSC	B0207.4. c. elegans.
Organism-specific databases
CTD	172268.
WormBase	B0207.4; CE24761; WBGene00000099; air-2.
Phylogenomic databases
eggNOG	meNOG04012.
GeneTree	EMGT00050000001100.
HOGENOM	HBG755340.
InParanoid	O01427.
OMA	VPTGAQD.
PhylomeDB	O01427.
Gene expression databases
ArrayExpress	O01427.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_kinase-like_dom. IPR008271. Ser/Thr_kinase_AS. IPR002290. Ser/Thr_kinase_dom. [Graphical view]
KO	K11481.
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	874753.

Entry information

Entry name

AIR2_CAEEL

Accession

Primary (citable) accession number: O01427

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	May 1, 2000
Last modified:	December 14, 2011
This is version 105 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project