Aurora/Ipl1-related protein kinase 2 - Caenorhabditis elegans

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Reviewed, UniProtKB/Swiss-Prot O01427 (AIR2_CAEEL)

Last modified June 16, 2009. Version 80. History...

Clusters with 100%, 90%, 50% identity |

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Aurora/Ipl1-related protein kinase 2
    EC=2.7.11.1
Alternative name(s):
    Aurora-B

Gene names

Name:	air-2
Synonyms:	stu-7
ORF Names:	B0207.4

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

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Protein attributes

Sequence length	305 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Serine/threonine-protein kinase which mediates both meiotic and mitotic chromosome segregation. Required for histone H3 'Ser-10' phosphorylation. Phosphorylates tlk-1 at 'Ser-634', which enhances its activity. Phosphorylates zen-4 at 'Ser-680'. Ref.1 Ref.3 Ref.4 Ref.5 Ref.6 Ref.7 Ref.8 Ref.9 Ref.11 Ref.12
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subunit structure	Interacts with zen-4 and icp-1. Part of a complex containing at least air-2; icp-1; csc-1 and bir-1. Interacts with tlk-1 and bmk-1. Ref.5 Ref.6 Ref.12 Ref.13
Subcellular location	Note: Meiotic and mitotic chromosomes. During each division, relocates to the midbody microtubules. Ref.1
Developmental stage	Present during gametogenesis and throughout embryogenesis (at protein level). Ref.1
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily. Contains 1 protein kinase domain.

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Ontologies

Keywords
Biological process	Cell cycle Cell division Chromosome partition Meiosis Mitosis
Cellular component	Chromosomal protein
Ligand	ATP-binding Nucleotide-binding
Molecular function	Chromatin regulator Developmental protein Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell division Inferred from electronic annotation. Source: UniProtKB-KW chromatin modification Inferred from electronic annotation. Source: UniProtKB-KW chromosome segregation Inferred from electronic annotation. Source: UniProtKB-KW embryonic development ending in birth or egg hatching Ref.5 Ref.8 Inferred from mutant phenotype. Source: WormBase hermaphrodite genitalia development Inferred from mutant phenotype. Source: WormBase meiosis Ref.8 Ref.9 Inferred from mutant phenotype. Source: WormBase mitosis Inferred from electronic annotation. Source: UniProtKB-KW mitotic spindle organization Ref.7 Inferred from mutant phenotype. Source: WormBase protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct	Notes
icp-1	Q9GSQ0	1	EBI-312947,EBI-312981

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 305

305

Aurora/Ipl1-related protein kinase 2

PRO_0000268640

Regions

Domain

30 – 280

251

Protein kinase

Nucleotide binding

36 – 44

ATP By similarity

Sites

Active site

153

Proton acceptor By similarity

Binding site

ATP By similarity

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Sequences

Sequence

Length

Mass (Da)

Tools

O01427-1 [UniParc].

Last modified May 1, 2000. Version 2.
Checksum: 1635EB60D2E14011
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FASTA

305

34,749

        10         20         30         40         50         60 
MENKPPVINL PEKETVNTPQ KGGKFTINDF EIGRPLGKGK FGSVYLARTK TGHFHVAIKV 

        70         80         90        100        110        120 
LFKSQLISGG VEHQLEREIE IQSHLNHPNI IKLYTYFWDA KKIYLVLEYA PGGEMYKQLT 

       130        140        150        160        170        180 
VSKRFSEPTA AKYMYEIADA LSYCHRKNVI HRDIKPENLL IGSQGELKIG DFGWSVHAPS 

       190        200        210        220        230        240 
NKRQTMCGTM DYLPPEMVNG ADHSDAVDLW AIGVLCYEFL VGKPPFEHED QSKTYAAIKA 

       250        260        270        280        290        300 
ARFTYPDSVK KGARDLIGRL LVVDPKARCT LEQVKEHYWI QGMMEAKIRA EKQQKIEKEA 


SLRNH

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"AIR-2: an Aurora/Ipl1-related protein kinase associated with chromosomes and midbody microtubules is required for polar body extrusion and cytokinesis in Caenorhabditis elegans embryos." Schumacher J.M., Golden A., Donovan P.J. J. Cell Biol. 143:1635-1646(1998) [PubMed: 9852156] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE. Strain: Bristol N2.
[2]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
[3]	"Stu-7/air-2 is a C. elegans aurora homologue essential for chromosome segregation during embryonic and post-embryonic development." Woollard A., Hodgkin J. Mech. Dev. 82:95-108(1999) [PubMed: 10354474] [Abstract] Cited for: IDENTIFICATION, FUNCTION.
[4]	"Mitotic phosphorylation of histone H3 is governed by Ipl1/aurora kinase and Glc7/PP1 phosphatase in budding yeast and nematodes." Hsu J.-Y., Sun Z.-W., Li X., Reuben M., Tatchell K., Bishop D.K., Grushcow J.M., Brame C.J., Caldwell J.A., Hunt D.F., Lin R., Smith M.M., Allis C.D. Cell 102:279-291(2000) [PubMed: 10975519] [Abstract] Cited for: FUNCTION.
[5]	"The aurora-related kinase AIR-2 recruits ZEN-4/CeMKLP1 to the mitotic spindle at metaphase and is required for cytokinesis." Severson A.F., Hamill D.R., Carter J.C., Schumacher J., Bowerman B. Curr. Biol. 10:1162-1171(2000) [PubMed: 11050384] [Abstract] Cited for: FUNCTION, INTERACTION WITH ZEN-4.
[6]	"Incenp and an aurora-like kinase form a complex essential for chromosome segregation and efficient completion of cytokinesis." Kaitna S., Mendoza M., Jantsch-Plunger V., Glotzer M. Curr. Biol. 10:1172-1181(2000) [PubMed: 11050385] [Abstract] Cited for: FUNCTION, INTERACTION WITH ICP-1.
[7]	"The survivin-like C. elegans BIR-1 protein acts with the Aurora-like kinase AIR-2 to affect chromosomes and the spindle midzone." Speliotes E.K., Uren A., Vaux D., Horvitz H.R. Mol. Cell 6:211-223(2000) [PubMed: 10983970] [Abstract] Cited for: FUNCTION.
[8]	"The aurora B kinase AIR-2 regulates kinetochores during mitosis and is required for separation of homologous Chromosomes during meiosis." Kaitna S., Pasierbek P., Jantsch M., Loidl J., Glotzer M. Curr. Biol. 12:798-812(2002) [PubMed: 12015116] [Abstract] Cited for: FUNCTION.
[9]	"The aurora kinase AIR-2 functions in the release of chromosome cohesion in Caenorhabditis elegans meiosis." Rogers E., Bishop J.D., Waddle J.A., Schumacher J.M., Lin R. J. Cell Biol. 157:219-229(2002) [PubMed: 11940606] [Abstract] Cited for: FUNCTION.
[10]	"CSC-1: a subunit of the Aurora B kinase complex that binds to the survivin-like protein BIR-1 and the incenp-like protein ICP-1." Romano A., Guse A., Krascenicova I., Schnabel H., Schnabel R., Glotzer M. J. Cell Biol. 161:229-236(2003) [PubMed: 12707312] [Abstract] Cited for: IDENTIFICATION IN A COMPLEX WITH ICP-1; CSC-1 AND BIR-1.
[11]	"Phosphorylation of ZEN-4/MKLP1 by aurora B regulates completion of cytokinesis." Guse A., Mishima M., Glotzer M. Curr. Biol. 15:778-786(2005) [PubMed: 15854913] [Abstract] Cited for: FUNCTION.
[12]	"The C. elegans Tousled-like kinase contributes to chromosome segregation as a substrate and regulator of the Aurora B kinase." Han Z., Riefler G.M., Saam J.R., Mango S.E., Schumacher J.M. Curr. Biol. 15:894-904(2005) [PubMed: 15916946] [Abstract] Cited for: FUNCTION, INTERACTION WITH TLK-1.
[13]	"The Caenorhabditis elegans Aurora B kinase AIR-2 phosphorylates and is required for the localization of a BimC kinesin to meiotic and mitotic spindles." Bishop J.D., Han Z., Schumacher J.M. Mol. Biol. Cell 16:742-756(2005) [PubMed: 15548597] [Abstract] Cited for: INTERACTION WITH BMK-1.
+	Additional computationally mapped references.

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Cross-references

Sequence databases
	AF071207 mRNA. Translation: AAC70945.1. U97196 Genomic DNA. Translation: AAB52459.2.
PIR	B87790. T43221.
RefSeq	NP_491714.1.
UniGene	Cel.6613
3D structure databases
HSSP	HSSP built from PDB template 1OL6 based on UniProtKB O14965.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:25477N.
IntAct	O01427. 5 interactions.
Genome annotation databases
Ensembl	B0207.4. Caenorhabditis elegans. [Contig view]
GeneID	172268.
KEGG	cel:B0207.4.
NMPDR	fig\|6239.3.peg.1382.
Organism-specific databases
WormBase	WBGene00000099. air-2.
WormPep	B0207.4. CE24761. [WorfDB]
Phylogenomic databases
OMA	O01427. TATYMEE.
Enzyme and pathway databases
BRENDA	2.7.11.1. 672.
Gene expression databases
ArrayExpress	O01427.
Family and domain databases
InterPro	IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	874753.

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Entry information

Entry name

AIR2_CAEEL

Accession

Primary (citable) accession number: O01427

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	May 1, 2000
Last modified:	June 16, 2009
This is version 80 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project

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Relevant documents

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormPep

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents