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Protein

Aurora/IPL1-related protein kinase 2

Gene

air-2

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Serine/threonine-protein kinase which mediates both meiotic and mitotic chromosome segregation. Required for histone H3 'Ser-10' phosphorylation (PubMed:9852156, PubMed:10354474, PubMed:10975519, PubMed:11050384, PubMed:11050385, PubMed:10983970, PubMed:12015116, PubMed:11940606). Phosphorylates tlk-1 at 'Ser-634', which enhances its activity (PubMed:15916946). Phosphorylates zen-4 at 'Ser-680'(PubMed:15854913). Required for the recruitment of bub-1 to the ring-shaped domain between chromosomes during meiotic anaphase I (PubMed:20729837).11 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei59 – 591ATPPROSITE-ProRule annotation
Active sitei153 – 1531Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi36 – 449ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ATPase binding Source: WormBase
  • ATP binding Source: UniProtKB-KW
  • histone serine kinase activity Source: GO_Central
  • kinesin binding Source: WormBase
  • protein kinase activity Source: WormBase
  • protein serine/threonine kinase activity Source: WormBase

GO - Biological processi

  • chromosome condensation Source: WormBase
  • chromosome segregation Source: WormBase
  • establishment of protein localization Source: WormBase
  • meiotic spindle midzone assembly Source: UniProtKB
  • mitotic spindle midzone assembly Source: WormBase
  • multicellular organism development Source: UniProtKB-KW
  • peptidyl-serine phosphorylation Source: WormBase
  • peptidyl-threonine phosphorylation Source: WormBase
  • protein autophosphorylation Source: WormBase
  • protein phosphorylation Source: WormBase
  • regulation of actomyosin contractile ring contraction Source: WormBase
Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator, Developmental protein, Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Cell cycle, Cell division, Chromosome partition, Meiosis, Mitosis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

SignaLinkiO01427.

Names & Taxonomyi

Protein namesi
Recommended name:
Aurora/IPL1-related protein kinase 2 (EC:2.7.11.1)
Alternative name(s):
Serine/threonine-protein kinase aurora-B
Gene namesi
Name:air-2
Synonyms:stu-7
ORF Names:B0207.4
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome I

Organism-specific databases

WormBaseiB0207.4; CE24761; WBGene00000099; air-2.

Subcellular locationi

GO - Cellular componenti

  • chromosome passenger complex Source: GO_Central
  • condensed chromosome Source: WormBase
  • condensed nuclear chromosome Source: WormBase
  • condensed nuclear chromosome, centromeric region Source: GO_Central
  • cytoplasm Source: UniProtKB-KW
  • midbody Source: UniProtKB-SubCell
  • spindle Source: WormBase
  • spindle microtubule Source: GO_Central
  • spindle midzone Source: WormBase
  • spindle pole centrosome Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Cytoplasm, Cytoskeleton

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi155 – 1551K → R: Reduced in vitro sumo-1 conjugation. 1 Publication
Mutagenesisi168 – 1681K → R: Reduced in vitro sumo-1 conjugation. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 305305Aurora/IPL1-related protein kinase 2PRO_0000268640Add
BLAST

Proteomic databases

EPDiO01427.
PaxDbiO01427.
PRIDEiO01427.

Expressioni

Developmental stagei

Present during gametogenesis and throughout embryogenesis (at protein level).1 Publication

Gene expression databases

BgeeiWBGene00000099.

Interactioni

Subunit structurei

Interacts with zen-4 and icp-1. Part of a complex containing at least air-2; icp-1; csc-1 and bir-1. Interacts with tlk-1 and bmk-1.5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
icp-1G5EE374EBI-312947,EBI-312981
lin-10O175832EBI-312947,EBI-313389
tlk-1P343143EBI-312947,EBI-3890382

GO - Molecular functioni

  • ATPase binding Source: WormBase
  • kinesin binding Source: WormBase

Protein-protein interaction databases

BioGridi37722. 8 interactions.
DIPiDIP-25477N.
IntActiO01427. 7 interactions.
MINTiMINT-229556.
STRINGi6239.B0207.4.

Structurei

3D structure databases

ProteinModelPortaliO01427.
SMRiO01427. Positions 24-280.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini30 – 280251Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0580. Eukaryota.
ENOG410XNRB. LUCA.
GeneTreeiENSGT00770000120530.
HOGENOMiHOG000233016.
InParanoidiO01427.
KOiK11479.
OMAiTYFWDAK.
OrthoDBiEOG091G0EU2.
PhylomeDBiO01427.

Family and domain databases

InterProiIPR030616. Aur.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR24350. PTHR24350. 1 hit.
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O01427-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MENKPPVINL PEKETVNTPQ KGGKFTINDF EIGRPLGKGK FGSVYLARTK
60 70 80 90 100
TGHFHVAIKV LFKSQLISGG VEHQLEREIE IQSHLNHPNI IKLYTYFWDA
110 120 130 140 150
KKIYLVLEYA PGGEMYKQLT VSKRFSEPTA AKYMYEIADA LSYCHRKNVI
160 170 180 190 200
HRDIKPENLL IGSQGELKIG DFGWSVHAPS NKRQTMCGTM DYLPPEMVNG
210 220 230 240 250
ADHSDAVDLW AIGVLCYEFL VGKPPFEHED QSKTYAAIKA ARFTYPDSVK
260 270 280 290 300
KGARDLIGRL LVVDPKARCT LEQVKEHYWI QGMMEAKIRA EKQQKIEKEA

SLRNH
Length:305
Mass (Da):34,749
Last modified:May 1, 2000 - v2
Checksum:i1635EB60D2E14011
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF071207 mRNA. Translation: AAC70945.1.
FO080115 Genomic DNA. Translation: CCD61317.1.
PIRiB87790.
T43221.
RefSeqiNP_491714.1. NM_059313.5.
UniGeneiCel.6613.

Genome annotation databases

EnsemblMetazoaiB0207.4; B0207.4; WBGene00000099.
GeneIDi172268.
KEGGicel:CELE_B0207.4.
UCSCiB0207.4. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF071207 mRNA. Translation: AAC70945.1.
FO080115 Genomic DNA. Translation: CCD61317.1.
PIRiB87790.
T43221.
RefSeqiNP_491714.1. NM_059313.5.
UniGeneiCel.6613.

3D structure databases

ProteinModelPortaliO01427.
SMRiO01427. Positions 24-280.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi37722. 8 interactions.
DIPiDIP-25477N.
IntActiO01427. 7 interactions.
MINTiMINT-229556.
STRINGi6239.B0207.4.

Proteomic databases

EPDiO01427.
PaxDbiO01427.
PRIDEiO01427.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiB0207.4; B0207.4; WBGene00000099.
GeneIDi172268.
KEGGicel:CELE_B0207.4.
UCSCiB0207.4. c. elegans.

Organism-specific databases

CTDi172268.
WormBaseiB0207.4; CE24761; WBGene00000099; air-2.

Phylogenomic databases

eggNOGiKOG0580. Eukaryota.
ENOG410XNRB. LUCA.
GeneTreeiENSGT00770000120530.
HOGENOMiHOG000233016.
InParanoidiO01427.
KOiK11479.
OMAiTYFWDAK.
OrthoDBiEOG091G0EU2.
PhylomeDBiO01427.

Enzyme and pathway databases

SignaLinkiO01427.

Miscellaneous databases

PROiO01427.

Gene expression databases

BgeeiWBGene00000099.

Family and domain databases

InterProiIPR030616. Aur.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR24350. PTHR24350. 1 hit.
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAIR2_CAEEL
AccessioniPrimary (citable) accession number: O01427
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: May 1, 2000
Last modified: September 7, 2016
This is version 144 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.