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Protein

Aurora/IPL1-related protein kinase 2

Gene

air-2

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Serine/threonine-protein kinase component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of chromosome segregation and cytokinesis (PubMed:12707312, PubMed:11050385, PubMed:10983970, PubMed:9852156, PubMed:10354474, PubMed:10975519, PubMed:11050384). The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation (PubMed:12707312, PubMed:10983970). Required for histone H3 'Ser-10' phosphorylation (PubMed:9852156, PubMed:10354474, PubMed:10975519, PubMed:11050384, PubMed:11050385, PubMed:10983970, PubMed:12015116, PubMed:11940606). Phosphorylates tlk-1 at 'Ser-634', which enhances its activity (PubMed:15916946). Phosphorylates zen-4 at 'Ser-680'(PubMed:15854913). Required for the recruitment of bub-1 to the ring-shaped domain between chromosomes during meiotic anaphase I (PubMed:20729837). Also required for the localization of the condensin I complex subunit smc-4 to mitotic chromosomes (PubMed:11914278). Acts at the spindle midzone and the midbody to prevent cleavage furrow regression upon chromatin obstructions during cytokinesis (PubMed:23684975).14 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei59ATPPROSITE-ProRule annotation1
Active sitei153Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi36 – 44ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATPase binding Source: WormBase
  • ATP binding Source: UniProtKB-KW
  • histone serine kinase activity Source: GO_Central
  • kinesin binding Source: WormBase
  • protein kinase activity Source: WormBase
  • protein serine/threonine kinase activity Source: WormBase

GO - Biological processi

  • chromosome condensation Source: WormBase
  • chromosome segregation Source: WormBase
  • establishment of protein localization Source: WormBase
  • meiotic spindle midzone assembly Source: UniProtKB
  • mitotic spindle midzone assembly Source: WormBase
  • multicellular organism development Source: UniProtKB-KW
  • peptidyl-serine phosphorylation Source: WormBase
  • peptidyl-threonine phosphorylation Source: WormBase
  • protein autophosphorylation Source: WormBase
  • protein phosphorylation Source: WormBase
  • regulation of actomyosin contractile ring contraction Source: WormBase

Keywordsi

Molecular functionChromatin regulator, Developmental protein, Kinase, Serine/threonine-protein kinase, Transferase
Biological processCell cycle, Cell division, Chromosome partition, Meiosis, Mitosis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-CEL-8854050. FBXL7 down-regulates AURKA during mitotic entry and in early mitosis.
SignaLinkiO01427.

Names & Taxonomyi

Protein namesi
Recommended name:
Aurora/IPL1-related protein kinase 2 (EC:2.7.11.1)
Alternative name(s):
Serine/threonine-protein kinase aurora-B
Gene namesi
Name:air-2
Synonyms:stu-7
ORF Names:B0207.4
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome I

Organism-specific databases

WormBaseiB0207.4; CE24761; WBGene00000099; air-2.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chromosome, Cytoplasm, Cytoskeleton

Pathology & Biotechi

Disruption phenotypei

RNAi-mediated knockdown disrupts the localization of the condensin subunit smc-4 to mitotic chromosomes. Causes failure in mitotic chromosome segregation and cytokinesis, leading to aneuploidy.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi155K → R: Reduced in vitro sumo-1 conjugation. 1 Publication1
Mutagenesisi168K → R: Reduced in vitro sumo-1 conjugation. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002686401 – 305Aurora/IPL1-related protein kinase 2Add BLAST305

Post-translational modificationi

Phosphorylated. Increased phosphorylation upon chromatin obstructions at anaphase.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO01427.
PaxDbiO01427.
PeptideAtlasiO01427.

Expressioni

Developmental stagei

Present during gametogenesis and throughout embryogenesis (at protein level).1 Publication

Gene expression databases

BgeeiWBGene00000099.

Interactioni

Subunit structurei

Component of the CPC complex which consists of icp-1; csc-1; bir-1 and air-2 (PubMed:12707312). Within the complex, interacts with icp-1; csc-1 and bir-1 (PubMed:12707312, PubMed:11050385). Interacts with zen-4 (PubMed:11050384). Interacts with tlk-1 and bmk-1 (PubMed:15548597, PubMed:15916946).5 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • ATPase binding Source: WormBase
  • kinesin binding Source: WormBase

Protein-protein interaction databases

BioGridi37722. 8 interactors.
DIPiDIP-25477N.
IntActiO01427. 7 interactors.
MINTiMINT-229556.
STRINGi6239.B0207.4.

Structurei

3D structure databases

ProteinModelPortaliO01427.
SMRiO01427.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini30 – 280Protein kinasePROSITE-ProRule annotationAdd BLAST251

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0580. Eukaryota.
ENOG410XNRB. LUCA.
GeneTreeiENSGT00870000136439.
HOGENOMiHOG000233016.
InParanoidiO01427.
KOiK11479.
OMAiPYGRQTT.
OrthoDBiEOG091G0EU2.
PhylomeDBiO01427.

Family and domain databases

InterProiView protein in InterPro
IPR030616. Aur.
IPR011009. Kinase-like_dom_sf.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
PANTHERiPTHR24350. PTHR24350. 1 hit.
PfamiView protein in Pfam
PF00069. Pkinase. 1 hit.
SMARTiView protein in SMART
SM00220. S_TKc. 1 hit.
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiView protein in PROSITE
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.

Sequencei

Sequence statusi: Complete.

O01427-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MENKPPVINL PEKETVNTPQ KGGKFTINDF EIGRPLGKGK FGSVYLARTK
60 70 80 90 100
TGHFHVAIKV LFKSQLISGG VEHQLEREIE IQSHLNHPNI IKLYTYFWDA
110 120 130 140 150
KKIYLVLEYA PGGEMYKQLT VSKRFSEPTA AKYMYEIADA LSYCHRKNVI
160 170 180 190 200
HRDIKPENLL IGSQGELKIG DFGWSVHAPS NKRQTMCGTM DYLPPEMVNG
210 220 230 240 250
ADHSDAVDLW AIGVLCYEFL VGKPPFEHED QSKTYAAIKA ARFTYPDSVK
260 270 280 290 300
KGARDLIGRL LVVDPKARCT LEQVKEHYWI QGMMEAKIRA EKQQKIEKEA

SLRNH
Length:305
Mass (Da):34,749
Last modified:May 1, 2000 - v2
Checksum:i1635EB60D2E14011
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF071207 mRNA. Translation: AAC70945.1.
FO080115 Genomic DNA. Translation: CCD61317.1.
PIRiB87790.
T43221.
RefSeqiNP_491714.1. NM_059313.5.
UniGeneiCel.6613.

Genome annotation databases

EnsemblMetazoaiB0207.4; B0207.4; WBGene00000099.
GeneIDi172268.
KEGGicel:CELE_B0207.4.
UCSCiB0207.4. c. elegans.

Similar proteinsi

Entry informationi

Entry nameiAIR2_CAEEL
AccessioniPrimary (citable) accession number: O01427
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: May 1, 2000
Last modified: November 22, 2017
This is version 154 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families