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Protein

Peptidylglycine alpha-hydroxylating monooxygenase

Gene

Phm

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Monooxygenase that catalyzes an essential reaction in C-terminal alpha-amidation of peptides. Produces an unstable peptidyl(2-hydroxyglycine) intermediate. C-terminal amidation of peptides is required for normal developmental transitions and for biosynthesis of secretory peptides throughout the life.2 Publications

Catalytic activityi

Peptidylglycine + ascorbate + O2 = peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O.1 Publication

Cofactori

Cu2+1 PublicationNote: Binds 2 copper ions per subunit.1 Publication

Kineticsi

  1. KM=2.2 µM for alpha-N-acetyl-Tyr-Val-Gly1 Publication

    pH dependencei

    Optimum pH is 5.0.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Metal bindingi95Copper ABy similarity1
    Metal bindingi96Copper ABy similarity1
    Metal bindingi172Copper ABy similarity1
    Metal bindingi241Copper BBy similarity1
    Metal bindingi243Copper BBy similarity1
    Metal bindingi317Copper BBy similarity1

    GO - Molecular functioni

    GO - Biological processi

    • memory Source: FlyBase
    • multicellular organism reproduction Source: FlyBase
    • peptide metabolic process Source: InterPro
    • regulation of imaginal disc-derived wing size Source: FlyBase
    • response to fungus Source: FlyBase

    Keywordsi

    Molecular functionMonooxygenase, Oxidoreductase
    LigandCopper, Metal-binding

    Enzyme and pathway databases

    SignaLinkiO01404

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Peptidylglycine alpha-hydroxylating monooxygenase (EC:1.14.17.3)
    Short name:
    dPHM
    Gene namesi
    Name:Phm
    ORF Names:CG3832
    OrganismiDrosophila melanogaster (Fruit fly)
    Taxonomic identifieri7227 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    Proteomesi
    • UP000000803 Componenti: Chromosome 2R

    Organism-specific databases

    FlyBaseiFBgn0283509 Phm

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Secreted

    Pathology & Biotechi

    Disruption phenotypei

    Death as late embryos with morphological defects that resemble those of animals with mutations in genes of the ecdysone-inducible regulatory circuit. Amidated peptides are largely absent but peptide precursors, a nonamidated neuropeptide, nonpeptide transmitters, and other peptide biosynthetic enzymes are detected.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Signal peptidei1 – 24Sequence analysisAdd BLAST24
    ChainiPRO_000024857125 – 365Peptidylglycine alpha-hydroxylating monooxygenaseAdd BLAST341

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Disulfide bondi69 ↔ 114By similarity
    Glycosylationi88N-linked (GlcNAc...) asparagineSequence analysis1
    Disulfide bondi102 ↔ 129By similarity
    Glycosylationi280N-linked (GlcNAc...) asparagineSequence analysis1
    Disulfide bondi297 ↔ 318By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiO01404
    PRIDEiO01404

    Expressioni

    Tissue specificityi

    Expressed in the central nervous system (CNS) in a small number of CNS neurons (approximately a few hundred). Expression is present both in cell bodies and within neuropil regions. It is strongly expressed in neuroendocrine neurons (at protein level).1 Publication

    Inductioni

    Transcriptionally regulated by DIMM.1 Publication

    Gene expression databases

    BgeeiFBgn0019948
    ExpressionAtlasiO01404 differential
    GenevisibleiO01404 DM

    Interactioni

    Protein-protein interaction databases

    BioGridi63409, 6 interactors
    IntActiO01404, 5 interactors
    STRINGi7227.FBpp0072112

    Structurei

    3D structure databases

    ProteinModelPortaliO01404
    SMRiO01404
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiKOG3567 Eukaryota
    ENOG410XS0X LUCA
    GeneTreeiENSGT00530000063085
    InParanoidiO01404
    KOiK00504
    OMAiFEPNATM
    OrthoDBiEOG091G0DV8
    PhylomeDBiO01404

    Family and domain databases

    Gene3Di2.60.120.230, 1 hit
    2.60.120.310, 1 hit
    InterProiView protein in InterPro
    IPR014784 Cu2_ascorb_mOase-like_C
    IPR014783 Cu2_ascorb_mOase_CS-2
    IPR000323 Cu2_ascorb_mOase_N
    IPR036939 Cu2_ascorb_mOase_N_sf
    IPR024548 Cu2_monoox_C
    IPR000720 PHM/PAL
    IPR008977 PHM/PNGase_F_dom_sf
    PfamiView protein in Pfam
    PF03712 Cu2_monoox_C, 1 hit
    PF01082 Cu2_monooxygen, 1 hit
    PRINTSiPR00790 PAMONOXGNASE
    SUPFAMiSSF49742 SSF49742, 2 hits
    PROSITEiView protein in PROSITE
    PS00085 CU2_MONOOXYGENASE_2, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O01404-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MPRISEIAAS VGLLLLIGVI SVDGLVKEGD YQNSLYQQNL ESNSATGATA
    60 70 80 90 100
    SFPFLMPNVS PQTPDLYLCT PIKVDPTTTY YIVGFNPNAT MNTAHHMLLY
    110 120 130 140 150
    GCGEPGTSKT TWNCGEMNRA SQEESASPCG PHSNSQIVYA WARDAQKLNL
    160 170 180 190 200
    PEGVGFKVGK NSPIKYLVLQ VHYAHIDKFK DGSTDDSGVF LDYTEEPRKK
    210 220 230 240 250
    LAGTLLLGTD GQIPAMKTEH LETACEVNEQ KVLHPFAYRV HTHGLGKVVS
    260 270 280 290 300
    GYRVRTNSDG EQEWLQLGKR DPLTPQMFYN TSNTDPIIEG DKIAVRCTMQ
    310 320 330 340 350
    STRHRTTKIG PTNEDEMCNF YLMYYVDHGE TLNMKFCFSQ GAPYYFWSNP
    360
    DSGLHNIPHI EASTL
    Length:365
    Mass (Da):40,564
    Last modified:November 1, 1998 - v2
    Checksum:i9368D9D92018C178
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF006663 mRNA Translation: AAB61676.1
    U77426 Genomic DNA Translation: AAB52566.1
    U77427 Genomic DNA Translation: AAB52567.1
    U77428 Genomic DNA Translation: AAB52568.2
    U77429 Genomic DNA Translation: AAB52569.1
    U77430 Genomic DNA Translation: AAB52570.1
    U77431 Genomic DNA Translation: AAB52571.1
    U77432 Genomic DNA Translation: AAB52572.1
    AE013599 Genomic DNA Translation: AAF47127.1
    AY069103 mRNA Translation: AAL39248.1
    RefSeqiNP_477225.1, NM_057877.4
    NP_726394.1, NM_166644.2
    UniGeneiDm.2282

    Genome annotation databases

    EnsemblMetazoaiFBtr0072202; FBpp0072111; FBgn0283509
    FBtr0072203; FBpp0072112; FBgn0283509
    GeneIDi37823
    KEGGidme:Dmel_CG3832

    Similar proteinsi

    Entry informationi

    Entry nameiPHM_DROME
    AccessioniPrimary (citable) accession number: O01404
    Secondary accession number(s): O01402
    , O01403, O01405, O01406, O01407, O01408, Q7JNH8, Q7JNH9, Q7JNI0, Q7JNI1, Q7JNI2, Q7JNI3, Q7JNI4
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 5, 2006
    Last sequence update: November 1, 1998
    Last modified: March 28, 2018
    This is version 142 of the entry and version 2 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health