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Protein

Ubiquitin carboxyl-terminal hydrolase

Gene

UCH

Organism
Aplysia californica (California sea hare)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Ubiquitin-protein hydrolase is involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin.

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei90 – 901NucleophilePROSITE-ProRule annotation
Active sitei164 – 1641Proton donorPROSITE-ProRule annotation
Sitei179 – 1791Important for enzyme activityBy similarity

GO - Molecular functioni

  1. ubiquitin-specific protease activity Source: InterPro

GO - Biological processi

  1. ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Protein family/group databases

MEROPSiC12.003.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase (EC:3.4.19.12)
Alternative name(s):
Ubiquitin thioesterase
Gene namesi
Name:UCH
OrganismiAplysia californica (California sea hare)
Taxonomic identifieri6500 [NCBI]
Taxonomic lineageiEukaryotaMetazoaLophotrochozoaMolluscaGastropodaHeterobranchiaEuthyneuraEuopisthobranchiaAplysiomorphaAplysioideaAplysiidaeAplysia

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 214214Ubiquitin carboxyl-terminal hydrolasePRO_0000211068Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliO01391.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase C12 family.Curated

Family and domain databases

Gene3Di3.40.532.10. 1 hit.
InterProiIPR001578. Peptidase_C12_UCH.
[Graphical view]
PANTHERiPTHR10589. PTHR10589. 1 hit.
PfamiPF01088. Peptidase_C12. 1 hit.
[Graphical view]
PRINTSiPR00707. UBCTHYDRLASE.
PROSITEiPS00140. UCH_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O01391-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASEQRWIPL ESNPKVLNKY VHNLGMDAGW NFVDVFGLDP ELLAMVPRPA
60 70 80 90 100
AALVLLFPDD KETVNQLIGE YQSDYPDSLY YTKQTIGNAC GTVAIVHALA
110 120 130 140 150
NNENVIPFDA AKHFKTFLEK TKPLNPEERA KHLEQDNLMG AAHGDCAQEG
160 170 180 190 200
DTQAPSQDEH VKSHFVALVH CNGTLYELDG RKEAPVVHGT TSADTFLEDA
210
AEVVKKFMAR DPEI
Length:214
Mass (Da):23,703
Last modified:July 1, 1997 - v1
Checksum:i07ACDEDAADB05D6E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U90177 mRNA. Translation: AAB52410.1.
RefSeqiNP_001191493.1. NM_001204564.1.
UniGeneiAcl.28.

Genome annotation databases

GeneIDi100533253.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U90177 mRNA. Translation: AAB52410.1.
RefSeqiNP_001191493.1. NM_001204564.1.
UniGeneiAcl.28.

3D structure databases

ProteinModelPortaliO01391.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiC12.003.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100533253.

Organism-specific databases

CTDi100533253.

Family and domain databases

Gene3Di3.40.532.10. 1 hit.
InterProiIPR001578. Peptidase_C12_UCH.
[Graphical view]
PANTHERiPTHR10589. PTHR10589. 1 hit.
PfamiPF01088. Peptidase_C12. 1 hit.
[Graphical view]
PRINTSiPR00707. UBCTHYDRLASE.
PROSITEiPS00140. UCH_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Ubiquitin C-terminal hydrolase is an immediate-early gene essential for long-term facilitation in Aplysia."
    Hegde A.N., Inokuchi K., Pei W., Casadio A., Ghirardi M., Chain D.G., Martin K.C., Kandel E.R., Schwartz J.H.
    Cell 89:115-126(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiUCHL_APLCA
AccessioniPrimary (citable) accession number: O01391
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 1, 1997
Last modified: January 7, 2015
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.