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O01391

- UCHL_APLCA

UniProt

O01391 - UCHL_APLCA

Protein

Ubiquitin carboxyl-terminal hydrolase

Gene

UCH

Organism
Aplysia californica (California sea hare)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 72 (01 Oct 2014)
      Sequence version 1 (01 Jul 1997)
      Previous versions | rss
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    Functioni

    Ubiquitin-protein hydrolase is involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin.

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei90 – 901NucleophilePROSITE-ProRule annotation
    Active sitei164 – 1641Proton donorPROSITE-ProRule annotation
    Sitei179 – 1791Important for enzyme activityBy similarity

    GO - Molecular functioni

    1. ubiquitin-specific protease activity Source: InterPro

    GO - Biological processi

    1. ubiquitin-dependent protein catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Ubl conjugation pathway

    Protein family/group databases

    MEROPSiC12.003.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin carboxyl-terminal hydrolase (EC:3.4.19.12)
    Alternative name(s):
    Ubiquitin thioesterase
    Gene namesi
    Name:UCH
    OrganismiAplysia californica (California sea hare)
    Taxonomic identifieri6500 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaLophotrochozoaMolluscaGastropodaHeterobranchiaEuthyneuraEuopisthobranchiaAplysiomorphaAplysioideaAplysiidaeAplysia

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 214214Ubiquitin carboxyl-terminal hydrolasePRO_0000211068Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliO01391.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase C12 family.Curated

    Family and domain databases

    Gene3Di3.40.532.10. 1 hit.
    InterProiIPR001578. Peptidase_C12_UCH.
    [Graphical view]
    PANTHERiPTHR10589. PTHR10589. 1 hit.
    PfamiPF01088. Peptidase_C12. 1 hit.
    [Graphical view]
    PRINTSiPR00707. UBCTHYDRLASE.
    PROSITEiPS00140. UCH_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O01391-1 [UniParc]FASTAAdd to Basket

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    MASEQRWIPL ESNPKVLNKY VHNLGMDAGW NFVDVFGLDP ELLAMVPRPA    50
    AALVLLFPDD KETVNQLIGE YQSDYPDSLY YTKQTIGNAC GTVAIVHALA 100
    NNENVIPFDA AKHFKTFLEK TKPLNPEERA KHLEQDNLMG AAHGDCAQEG 150
    DTQAPSQDEH VKSHFVALVH CNGTLYELDG RKEAPVVHGT TSADTFLEDA 200
    AEVVKKFMAR DPEI 214
    Length:214
    Mass (Da):23,703
    Last modified:July 1, 1997 - v1
    Checksum:i07ACDEDAADB05D6E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U90177 mRNA. Translation: AAB52410.1.
    RefSeqiNP_001191493.1. NM_001204564.1.
    UniGeneiAcl.28.

    Genome annotation databases

    GeneIDi100533253.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U90177 mRNA. Translation: AAB52410.1 .
    RefSeqi NP_001191493.1. NM_001204564.1.
    UniGenei Acl.28.

    3D structure databases

    ProteinModelPortali O01391.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi C12.003.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 100533253.

    Organism-specific databases

    CTDi 100533253.

    Family and domain databases

    Gene3Di 3.40.532.10. 1 hit.
    InterProi IPR001578. Peptidase_C12_UCH.
    [Graphical view ]
    PANTHERi PTHR10589. PTHR10589. 1 hit.
    Pfami PF01088. Peptidase_C12. 1 hit.
    [Graphical view ]
    PRINTSi PR00707. UBCTHYDRLASE.
    PROSITEi PS00140. UCH_1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Ubiquitin C-terminal hydrolase is an immediate-early gene essential for long-term facilitation in Aplysia."
      Hegde A.N., Inokuchi K., Pei W., Casadio A., Ghirardi M., Chain D.G., Martin K.C., Kandel E.R., Schwartz J.H.
      Cell 89:115-126(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].

    Entry informationi

    Entry nameiUCHL_APLCA
    AccessioniPrimary (citable) accession number: O01391
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: July 1, 1997
    Last modified: October 1, 2014
    This is version 72 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3