Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

O01391 (UCHL_APLCA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ubiquitin carboxyl-terminal hydrolase

EC=3.4.19.12
Alternative name(s):
Ubiquitin thioesterase
Gene names
Name:UCH
OrganismAplysia californica (California sea hare)
Taxonomic identifier6500 [NCBI]
Taxonomic lineageEukaryotaMetazoaLophotrochozoaMolluscaGastropodaHeterobranchiaEuthyneuraEuopisthobranchiaAplysiomorphaAplysioideaAplysiidaeAplysia

Protein attributes

Sequence length214 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Ubiquitin-protein hydrolase is involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin.

Catalytic activity

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Subcellular location

Cytoplasm.

Sequence similarities

Belongs to the peptidase C12 family.

Ontologies

Keywords
   Biological processUbl conjugation pathway
   Cellular componentCytoplasm
   Molecular functionHydrolase
Protease
Thiol protease
Gene Ontology (GO)
   Biological_processubiquitin-dependent protein catabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionubiquitin-specific protease activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 214214Ubiquitin carboxyl-terminal hydrolase
PRO_0000211068

Sites

Active site901Nucleophile By similarity
Active site1641Proton donor By similarity
Site1791Important for enzyme activity By similarity

Sequences

Sequence LengthMass (Da)Tools
O01391 [UniParc].

Last modified July 1, 1997. Version 1.
Checksum: 07ACDEDAADB05D6E

FASTA21423,703
        10         20         30         40         50         60 
MASEQRWIPL ESNPKVLNKY VHNLGMDAGW NFVDVFGLDP ELLAMVPRPA AALVLLFPDD 

        70         80         90        100        110        120 
KETVNQLIGE YQSDYPDSLY YTKQTIGNAC GTVAIVHALA NNENVIPFDA AKHFKTFLEK 

       130        140        150        160        170        180 
TKPLNPEERA KHLEQDNLMG AAHGDCAQEG DTQAPSQDEH VKSHFVALVH CNGTLYELDG 

       190        200        210 
RKEAPVVHGT TSADTFLEDA AEVVKKFMAR DPEI 

« Hide

References

[1]"Ubiquitin C-terminal hydrolase is an immediate-early gene essential for long-term facilitation in Aplysia."
Hegde A.N., Inokuchi K., Pei W., Casadio A., Ghirardi M., Chain D.G., Martin K.C., Kandel E.R., Schwartz J.H.
Cell 89:115-126(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U90177 mRNA. Translation: AAB52410.1.
RefSeqNP_001191493.1. NM_001204564.1.
UniGeneAcl.28.

3D structure databases

ProteinModelPortalO01391.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSC12.003.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID100533253.

Organism-specific databases

CTD100533253.

Family and domain databases

Gene3D3.40.532.10. 1 hit.
InterProIPR001578. Peptidase_C12_UCH.
[Graphical view]
PANTHERPTHR10589. PTHR10589. 1 hit.
PfamPF01088. Peptidase_C12. 1 hit.
[Graphical view]
PRINTSPR00707. UBCTHYDRLASE.
PROSITEPS00140. UCH_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameUCHL_APLCA
AccessionPrimary (citable) accession number: O01391
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 1, 1997
Last modified: April 16, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries