O01391 (UCHL_APLCA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin carboxyl-terminal hydrolase
EC=3.4.19.12

Alternative name(s):
Ubiquitin thioesterase

Gene names

Name:

UCH

Organism

Aplysia californica (California sea hare)

Taxonomic identifier

6500 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Lophotrochozoa › Mollusca › Gastropoda › Heterobranchia › Euthyneura › Euopisthobranchia › Aplysiomorpha › Aplysioidea › Aplysiidae › Aplysia

Protein attributes

Sequence length	214 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Ubiquitin-protein hydrolase is involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin.
Catalytic activity	Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the peptidase C12 family.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Cytoplasm
Molecular function	Hydrolase Protease Thiol protease
Gene Ontology (GO)
Biological_process	ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	cysteine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin thiolesterase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 214	214	Ubiquitin carboxyl-terminal hydrolase		PRO_0000211068
Sites
	Active site	90	1	Nucleophile By similarity
	Active site	164	1	Proton donor By similarity
	Site	179	1	Important for enzyme activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	O01391 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: 07ACDEDAADB05D6E	FASTA	214	23,703
	10 20 30 40 50 60 MASEQRWIPL ESNPKVLNKY VHNLGMDAGW NFVDVFGLDP ELLAMVPRPA AALVLLFPDD 70 80 90 100 110 120 KETVNQLIGE YQSDYPDSLY YTKQTIGNAC GTVAIVHALA NNENVIPFDA AKHFKTFLEK 130 140 150 160 170 180 TKPLNPEERA KHLEQDNLMG AAHGDCAQEG DTQAPSQDEH VKSHFVALVH CNGTLYELDG 190 200 210 RKEAPVVHGT TSADTFLEDA AEVVKKFMAR DPEI

References

[1]

"Ubiquitin C-terminal hydrolase is an immediate-early gene essential for long-term facilitation in Aplysia." Hegde A.N., Inokuchi K., Pei W., Casadio A., Ghirardi M., Chain D.G., Martin K.C., Kandel E.R., Schwartz J.H. Cell 89:115-126(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	U90177 mRNA. Translation: AAB52410.1.
RefSeq	NP_001191493.1. NM_001204564.1.
UniGene	Acl.28.
3D structure databases
ProteinModelPortal	O01391.
ModBase	Search...
Protein family/group databases
MEROPS	C12.003.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	100533253.
Organism-specific databases
CTD	100533253.
Family and domain databases
Gene3D	3.40.532.10. 1 hit.
InterPro	IPR001578. Peptidase_C12. [Graphical view]
PANTHER	PTHR10589. PTHR10589. 1 hit.
Pfam	PF01088. Peptidase_C12. 1 hit. [Graphical view]
PRINTS	PR00707. UBCTHYDRLASE.
PROSITE	PS00140. UCH_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UCHL_APLCA

Accession

Primary (citable) accession number: O01391

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	July 1, 1997
Last modified:	April 3, 2013
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)