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Protein

Glyceraldehyde-3-phosphate dehydrogenase

Gene
N/A
Organism
Onchocerca volvulus
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH.PROSITE-ProRule annotation

Pathwayi: glycolysis

This protein is involved in step 1 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase (WBGene00248297), Glyceraldehyde-3-phosphate dehydrogenase, Glyceraldehyde-3-phosphate dehydrogenase (WBGene00248298)
  2. no protein annotated in this organism
  3. 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (ipgm-1)
  4. no protein annotated in this organism
  5. Pyruvate kinase (WBGene00237398), Pyruvate kinase (WBGene00246611), Pyruvate kinase (WBGene00237397), Pyruvate kinase (WBGene00237402)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei35 – 351NADBy similarity
Binding sitei84 – 841NAD; via carbonyl oxygenBy similarity
Active sitei157 – 1571NucleophilePROSITE-ProRule annotation
Sitei184 – 1841Activates thiol group during catalysisBy similarity
Binding sitei187 – 1871Glyceraldehyde 3-phosphateBy similarity
Binding sitei239 – 2391Glyceraldehyde 3-phosphateBy similarity
Binding sitei321 – 3211NADBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi13 – 142NADBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

NAD

Enzyme and pathway databases

UniPathwayiUPA00109; UER00184.

Names & Taxonomyi

Protein namesi
Recommended name:
Glyceraldehyde-3-phosphate dehydrogenase (EC:1.2.1.12)
Short name:
GAPDH
Alternative name(s):
Larval antigen OvB95
OrganismiOnchocerca volvulus
Taxonomic identifieri6282 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaSpiruridaFilarioideaOnchocercidaeOnchocerca
Proteomesi
  • UP000024404 Componenti: Unassembled WGS sequence

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 339339Glyceraldehyde-3-phosphate dehydrogenasePRO_0000145519Add
BLAST

Interactioni

Subunit structurei

Homotetramer.By similarity

Structurei

3D structure databases

ProteinModelPortaliO01360.
SMRiO01360. Positions 3-339.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni156 – 1583Glyceraldehyde 3-phosphate bindingBy similarity
Regioni216 – 2172Glyceraldehyde 3-phosphate bindingBy similarity

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006424. Glyceraldehyde-3-P_DH_1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR10836. PTHR10836. 1 hit.
PfamiPF02800. Gp_dh_C. 1 hit.
PF00044. Gp_dh_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000149. GAP_DH. 1 hit.
PRINTSiPR00078. G3PDHDRGNASE.
SMARTiSM00846. Gp_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01534. GAPDH-I. 1 hit.
PROSITEiPS00071. GAPDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O01360-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKPKIGING FGRIGRLVLR AAVEKDTVEV VAVNDPFINI DYMVYMFKYD
60 70 80 90 100
STHGRFKGHV SAEGGKLIVT NGKTTHQIAV HNSKDPAEIP WGVEGAEYVV
110 120 130 140 150
ESTGVFTHTE KASAHLKGGA KKVIISAPSA DAPMFVMGVN NDKYDKANNH
160 170 180 190 200
IISNASCTTN CLAPLAKVIH DKFGIIEGLM TTVHATTATQ KTVDGPSGKL
210 220 230 240 250
WRDGRGAGQN IIPASTGAAK AVGKVIPDLN GKLTGMASRV PTPDVSVVDL
260 270 280 290 300
TCRLQKGASM DEIKAAVKEA AAGPMKGILE YTEDQVVSSD FVGDPHSSIF
310 320 330
DALACISLNP NFVKLIAWYD NEYGYSNRVV DLISYNASK
Length:339
Mass (Da):36,180
Last modified:July 1, 1997 - v1
Checksum:i5BC933D05FD49B16
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti108 – 1081H → T in AAB52599 (Ref. 2) Curated
Sequence conflicti238 – 2381S → F in AAB52599 (Ref. 2) Curated
Sequence conflicti296 – 2961H → Y in AAB52599 (Ref. 2) Curated
Sequence conflicti305 – 3051C → R in AAB52599 (Ref. 2) Curated
Sequence conflicti330 – 3301V → I in AAB52599 (Ref. 2) Curated
Sequence conflicti336 – 3361N → I in AAB52599 (Ref. 2) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y09455 mRNA. Translation: CAA70607.1.
U96177 mRNA. Translation: AAB52599.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y09455 mRNA. Translation: CAA70607.1.
U96177 mRNA. Translation: AAB52599.1.

3D structure databases

ProteinModelPortaliO01360.
SMRiO01360. Positions 3-339.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00109; UER00184.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006424. Glyceraldehyde-3-P_DH_1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR10836. PTHR10836. 1 hit.
PfamiPF02800. Gp_dh_C. 1 hit.
PF00044. Gp_dh_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000149. GAP_DH. 1 hit.
PRINTSiPR00078. G3PDHDRGNASE.
SMARTiSM00846. Gp_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01534. GAPDH-I. 1 hit.
PROSITEiPS00071. GAPDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Schneider E.A., Gallin M.
    Submitted (NOV-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Onchocerca volvulus: molecular cloning and characterization of a larval antigen, OvB95 recognized by putatively immune individuals from Liberia and Ecuador."
    Joseph G.T., Huima T., Lustigman S.
    Submitted (APR-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Forest.

Entry informationi

Entry nameiG3P_ONCVO
AccessioniPrimary (citable) accession number: O01360
Secondary accession number(s): O01656
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: July 1, 1997
Last modified: December 9, 2015
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.