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O01326

- RNC_CAEEL

UniProt

O01326 - RNC_CAEEL

Protein

Ribonuclease 3

Gene

drsh-1

Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 107 (01 Oct 2014)
      Sequence version 2 (23 Jan 2002)
      Previous versions | rss
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    Functioni

    Executes the initial step of microRNA (miRNA) processing in the nucleus, that is the cleavage of pri-miRNA to release pre-miRNA. Involved in pre-rRNA processing. Cleaves double-strand RNA and does not cleave single-strand RNA. Involved in fertility. Required for the function or synthesis of the let-7 miRNA.1 Publication

    Catalytic activityi

    Endonucleolytic cleavage to 5'-phosphomonoester.

    Cofactori

    Magnesium or manganese.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi873 – 8731Magnesium or manganeseBy similarity
    Sitei939 – 9391Important for activityBy similarity
    Metal bindingi943 – 9431Magnesium or manganeseBy similarity
    Metal bindingi946 – 9461Magnesium or manganeseBy similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. ribonuclease III activity Source: UniProtKB
    3. RNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. cell differentiation Source: UniProtKB-KW
    2. gonadal mesoderm development Source: UniProtKB-KW
    3. primary miRNA processing Source: UniProtKB
    4. RNA phosphodiester bond hydrolysis Source: GOC
    5. RNA phosphodiester bond hydrolysis, endonucleolytic Source: GOC
    6. rRNA catabolic process Source: InterPro
    7. rRNA processing Source: UniProtKB-KW
    8. sexual reproduction Source: UniProtKB

    Keywords - Molecular functioni

    Developmental protein, Endonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    Differentiation, Gonadal differentiation, Ribosome biogenesis, rRNA processing

    Keywords - Ligandi

    Magnesium, Manganese, Metal-binding, RNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribonuclease 3 (EC:3.1.26.3)
    Alternative name(s):
    Protein drosha
    Ribonuclease III
    Short name:
    RNase III
    Gene namesi
    Name:drsh-1
    ORF Names:F26E4.10
    OrganismiCaenorhabditis elegans
    Taxonomic identifieri6239 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
    ProteomesiUP000001940: Chromosome I

    Organism-specific databases

    WormBaseiF26E4.10a; CE29039; WBGene00009163; drsh-1.
    F26E4.10b; CE46611; WBGene00009163; drsh-1.

    Subcellular locationi

    Nucleus Curated

    GO - Cellular componenti

    1. microprocessor complex Source: WormBase
    2. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Disruption phenotypei

    Sterility.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10861086Ribonuclease 3PRO_0000180469Add
    BLAST

    Proteomic databases

    PaxDbiO01326.

    Structurei

    3D structure databases

    ProteinModelPortaliO01326.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini607 – 781175RNase III 1Add
    BLAST
    Domaini833 – 957125RNase III 2Add
    BLAST
    Domaini984 – 105976DRBMAdd
    BLAST

    Sequence similaritiesi

    Contains 2 RNase III domains.Curated

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG0571.
    GeneTreeiENSGT00730000111052.
    HOGENOMiHOG000154167.
    InParanoidiO01326.
    OMAiEPILHNE.
    OrthoDBiEOG773XF7.
    PhylomeDBiO01326.

    Family and domain databases

    Gene3Di1.10.1520.10. 3 hits.
    3.30.160.20. 1 hit.
    HAMAPiMF_00104. RNase_III.
    InterProiIPR014720. dsRNA-bd_dom.
    IPR011907. RNase_III.
    IPR000999. RNase_III_dom.
    [Graphical view]
    PANTHERiPTHR11207. PTHR11207. 1 hit.
    PfamiPF00035. dsrm. 1 hit.
    PF14622. Ribonucleas_3_3. 1 hit.
    PF00636. Ribonuclease_3. 1 hit.
    [Graphical view]
    SMARTiSM00358. DSRM. 1 hit.
    SM00535. RIBOc. 2 hits.
    [Graphical view]
    SUPFAMiSSF69065. SSF69065. 3 hits.
    PROSITEiPS50137. DS_RBD. 1 hit.
    PS00517. RNASE_3_1. 2 hits.
    PS50142. RNASE_3_2. 2 hits.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform a (identifier: O01326-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSDEKISMTL NFPKHKRARR KKYQKEYQER HKEEMMQQLG RRFQNQPSTS     50
    SAPPDTVEKI PLPTESTSAL PFGDSPRLTE KDYETNYMID PPVVSTHSAE 100
    LIKSNRVVIK AEEAEKYMMI KAKSTTSKIL QDFQTKILET VKTKRRLQAD 150
    VPYIIHPCHS MKGRKTPKQK GGDESFTASD VSDDSNDSQD EASTSEPTNR 200
    QAPEADKTGE VKDEKQTCNR RNQQRKAKRL RNFEEKERQI TLLKKGIDRK 250
    KTHPNGIHPD ISFNEKGLGN EGPECRCPEP IKTCGLKHGY YAGEDKAIDC 300
    KKSNGENLHY YTLRVTPLPS ENQLYRTHMA INGEEFEFEG FSLITHAPLP 350
    DCMTRAPICK YSMDYEFQLV EEFMPDECFD PEDCDMLFEY IFHEIFEMLD 400
    FELRPKHIPS DVESCPMIHI MPRFVQTKDD LVQLWSSKTV LAYFTSKGSS 450
    EIMSPEDVNR LCDAQIDQFT RNTSKHKQSI VLNTKFKPSA IRADWFERDE 500
    EKKEVYVVHN AIRAQTYTAI SLPRIAFLEK TLNKMIQEKQ SSGVYNKDFE 550
    KTKNELEHLK RENRSARNLK LREPVAGFIE TGLKPDVAAH VVMTILACHH 600
    IRYNFSLDVF EEVIEYKFND RRVIELALMH SSFKSHYGTP IDHVKNMITN 650
    CGYRRKYGAE DKREKKRVAG IMSLFNIMKG TSGGEPILHN ERLEYLGDAV 700
    VELIVSHHLY FMLTHHFEGG LATYRTALVQ NRNLATLAKN CRIDEMLQYS 750
    HGADLINVAE FKHALANAFE AVMAAIYLDG GLAPCDVIFS KAMYGHQPVL 800
    KEKWDHINEH ELKREDPQGD RDLSFITPTL STFHALEERL GIQFNNIRLL 850
    AKAFTRRNIP NNDLTKGHNQ RLEWLGDSVL QLIVSDFLYR RFPYHHEGHM 900
    SLLRTSLVSN QTQAVVCDDL GFTEFVIKAP YKTPELKLKD KADLVEAFIG 950
    ALYVDRGIEH CRAFIRIVFC PRLKHFIESE KWNDAKSHLQ QWCLAMRDPS 1000
    SSEPDMPEYR VLGIEGPTNN RIFKIAVYYK GKRLASAAES NVHKAELRVA 1050
    ELALANLESM SFSKMKAKNN SWFQNMRRRL EQDTSD 1086

    Note: No experimental confirmation available.

    Length:1,086
    Mass (Da):125,334
    Last modified:January 23, 2002 - v2
    Checksum:i4A478120F88F8FB8
    GO
    Isoform b (identifier: O01326-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         668-669: Missing.
         1072-1074: Missing.

    Show »
    Length:1,081
    Mass (Da):124,702
    Checksum:iAEB0EBE694EF1830
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei668 – 6692Missing in isoform b. CuratedVSP_043603
    Alternative sequencei1072 – 10743Missing in isoform b. CuratedVSP_034688

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF160248 mRNA. Translation: AAD45518.1.
    Z81070 Genomic DNA. Translation: CAB03006.3.
    Z81070 Genomic DNA. Translation: CAP19331.2.
    PIRiT21420.
    RefSeqiNP_001122460.2. NM_001128988.2. [O01326-2]
    NP_492599.1. NM_060198.3. [O01326-1]
    UniGeneiCel.18868.

    Genome annotation databases

    EnsemblMetazoaiF26E4.10a; F26E4.10a; WBGene00009163. [O01326-1]
    GeneIDi172830.
    KEGGicel:CELE_F26E4.10.
    UCSCiF26E4.10a. c. elegans. [O01326-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF160248 mRNA. Translation: AAD45518.1 .
    Z81070 Genomic DNA. Translation: CAB03006.3 .
    Z81070 Genomic DNA. Translation: CAP19331.2 .
    PIRi T21420.
    RefSeqi NP_001122460.2. NM_001128988.2. [O01326-2 ]
    NP_492599.1. NM_060198.3. [O01326-1 ]
    UniGenei Cel.18868.

    3D structure databases

    ProteinModelPortali O01326.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PaxDbi O01326.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai F26E4.10a ; F26E4.10a ; WBGene00009163 . [O01326-1 ]
    GeneIDi 172830.
    KEGGi cel:CELE_F26E4.10.
    UCSCi F26E4.10a. c. elegans. [O01326-1 ]

    Organism-specific databases

    CTDi 172830.
    WormBasei F26E4.10a ; CE29039 ; WBGene00009163 ; drsh-1.
    F26E4.10b ; CE46611 ; WBGene00009163 ; drsh-1.

    Phylogenomic databases

    eggNOGi COG0571.
    GeneTreei ENSGT00730000111052.
    HOGENOMi HOG000154167.
    InParanoidi O01326.
    OMAi EPILHNE.
    OrthoDBi EOG773XF7.
    PhylomeDBi O01326.

    Miscellaneous databases

    NextBioi 877175.
    PROi O01326.

    Family and domain databases

    Gene3Di 1.10.1520.10. 3 hits.
    3.30.160.20. 1 hit.
    HAMAPi MF_00104. RNase_III.
    InterProi IPR014720. dsRNA-bd_dom.
    IPR011907. RNase_III.
    IPR000999. RNase_III_dom.
    [Graphical view ]
    PANTHERi PTHR11207. PTHR11207. 1 hit.
    Pfami PF00035. dsrm. 1 hit.
    PF14622. Ribonucleas_3_3. 1 hit.
    PF00636. Ribonuclease_3. 1 hit.
    [Graphical view ]
    SMARTi SM00358. DSRM. 1 hit.
    SM00535. RIBOc. 2 hits.
    [Graphical view ]
    SUPFAMi SSF69065. SSF69065. 3 hits.
    PROSITEi PS50137. DS_RBD. 1 hit.
    PS00517. RNASE_3_1. 2 hits.
    PS50142. RNASE_3_2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A novel type of RNase III family proteins in eukaryotes."
      Filippov V., Solovyev V., Filippova M., Gill S.S.
      Gene 245:213-221(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 227-1086 (ISOFORM A).
    2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
      The C. elegans sequencing consortium
      Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
      Strain: Bristol N2.
    3. "Processing of primary microRNAs by the Microprocessor complex."
      Denli A.M., Tops B.B.J., Plasterk R.H.A., Ketting R.F., Hannon G.J.
      Nature 432:231-235(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE.

    Entry informationi

    Entry nameiRNC_CAEEL
    AccessioniPrimary (citable) accession number: O01326
    Secondary accession number(s): A8WI03, O01327, Q9U9Q8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: January 23, 2002
    Last modified: October 1, 2014
    This is version 107 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programCaenorhabditis annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Caenorhabditis elegans
      Caenorhabditis elegans: entries, gene names and cross-references to WormBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3