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O01326 (RNC_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 106. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ribonuclease 3

EC=3.1.26.3
Alternative name(s):
Protein drosha
Ribonuclease III
Short name=RNase III
Gene names
Name:drsh-1
ORF Names:F26E4.10
OrganismCaenorhabditis elegans [Reference proteome]
Taxonomic identifier6239 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis

Protein attributes

Sequence length1086 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Executes the initial step of microRNA (miRNA) processing in the nucleus, that is the cleavage of pri-miRNA to release pre-miRNA. Involved in pre-rRNA processing. Cleaves double-strand RNA and does not cleave single-strand RNA. Involved in fertility. Required for the function or synthesis of the let-7 miRNA. Ref.3

Catalytic activity

Endonucleolytic cleavage to 5'-phosphomonoester. HAMAP-Rule MF_00104

Cofactor

Magnesium or manganese By similarity. HAMAP-Rule MF_00104

Subcellular location

Nucleus Probable HAMAP-Rule MF_00104.

Disruption phenotype

Sterility. Ref.3

Sequence similarities

Contains 1 DRBM (double-stranded RNA-binding) domain.

Contains 2 RNase III domains.

Ontologies

Keywords
   Biological processDifferentiation
Gonadal differentiation
Ribosome biogenesis
rRNA processing
   Cellular componentNucleus
   Coding sequence diversityAlternative splicing
   DomainRepeat
   LigandMagnesium
Manganese
Metal-binding
RNA-binding
   Molecular functionDevelopmental protein
Endonuclease
Hydrolase
Nuclease
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processRNA phosphodiester bond hydrolysis

Inferred from mutant phenotype Ref.3. Source: GOC

RNA phosphodiester bond hydrolysis, endonucleolytic

Inferred from mutant phenotype Ref.3. Source: GOC

cell differentiation

Inferred from electronic annotation. Source: UniProtKB-KW

gonadal mesoderm development

Inferred from electronic annotation. Source: UniProtKB-KW

primary miRNA processing

Inferred from mutant phenotype Ref.3. Source: UniProtKB

rRNA catabolic process

Inferred from electronic annotation. Source: InterPro

rRNA processing

Inferred from electronic annotation. Source: UniProtKB-KW

sexual reproduction

Inferred from mutant phenotype Ref.3. Source: UniProtKB

   Cellular_componentmicroprocessor complex

Inferred from sequence or structural similarity Ref.3. Source: WormBase

nucleus

Inferred by curator Ref.3. Source: UniProtKB

   Molecular_functionRNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

ribonuclease III activity

Inferred from mutant phenotype Ref.3. Source: UniProtKB

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform a (identifier: O01326-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: No experimental confirmation available.
Isoform b (identifier: O01326-2)

The sequence of this isoform differs from the canonical sequence as follows:
     668-669: Missing.
     1072-1074: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10861086Ribonuclease 3 HAMAP-Rule MF_00104
PRO_0000180469

Regions

Domain607 – 781175RNase III 1
Domain833 – 957125RNase III 2
Domain984 – 105976DRBM

Sites

Metal binding8731Magnesium or manganese By similarity
Metal binding9431Magnesium or manganese By similarity
Metal binding9461Magnesium or manganese By similarity
Site9391Important for activity By similarity

Natural variations

Alternative sequence668 – 6692Missing in isoform b.
VSP_043603
Alternative sequence1072 – 10743Missing in isoform b.
VSP_034688

Sequences

Sequence LengthMass (Da)Tools
Isoform a [UniParc].

Last modified January 23, 2002. Version 2.
Checksum: 4A478120F88F8FB8

FASTA1,086125,334
        10         20         30         40         50         60 
MSDEKISMTL NFPKHKRARR KKYQKEYQER HKEEMMQQLG RRFQNQPSTS SAPPDTVEKI 

        70         80         90        100        110        120 
PLPTESTSAL PFGDSPRLTE KDYETNYMID PPVVSTHSAE LIKSNRVVIK AEEAEKYMMI 

       130        140        150        160        170        180 
KAKSTTSKIL QDFQTKILET VKTKRRLQAD VPYIIHPCHS MKGRKTPKQK GGDESFTASD 

       190        200        210        220        230        240 
VSDDSNDSQD EASTSEPTNR QAPEADKTGE VKDEKQTCNR RNQQRKAKRL RNFEEKERQI 

       250        260        270        280        290        300 
TLLKKGIDRK KTHPNGIHPD ISFNEKGLGN EGPECRCPEP IKTCGLKHGY YAGEDKAIDC 

       310        320        330        340        350        360 
KKSNGENLHY YTLRVTPLPS ENQLYRTHMA INGEEFEFEG FSLITHAPLP DCMTRAPICK 

       370        380        390        400        410        420 
YSMDYEFQLV EEFMPDECFD PEDCDMLFEY IFHEIFEMLD FELRPKHIPS DVESCPMIHI 

       430        440        450        460        470        480 
MPRFVQTKDD LVQLWSSKTV LAYFTSKGSS EIMSPEDVNR LCDAQIDQFT RNTSKHKQSI 

       490        500        510        520        530        540 
VLNTKFKPSA IRADWFERDE EKKEVYVVHN AIRAQTYTAI SLPRIAFLEK TLNKMIQEKQ 

       550        560        570        580        590        600 
SSGVYNKDFE KTKNELEHLK RENRSARNLK LREPVAGFIE TGLKPDVAAH VVMTILACHH 

       610        620        630        640        650        660 
IRYNFSLDVF EEVIEYKFND RRVIELALMH SSFKSHYGTP IDHVKNMITN CGYRRKYGAE 

       670        680        690        700        710        720 
DKREKKRVAG IMSLFNIMKG TSGGEPILHN ERLEYLGDAV VELIVSHHLY FMLTHHFEGG 

       730        740        750        760        770        780 
LATYRTALVQ NRNLATLAKN CRIDEMLQYS HGADLINVAE FKHALANAFE AVMAAIYLDG 

       790        800        810        820        830        840 
GLAPCDVIFS KAMYGHQPVL KEKWDHINEH ELKREDPQGD RDLSFITPTL STFHALEERL 

       850        860        870        880        890        900 
GIQFNNIRLL AKAFTRRNIP NNDLTKGHNQ RLEWLGDSVL QLIVSDFLYR RFPYHHEGHM 

       910        920        930        940        950        960 
SLLRTSLVSN QTQAVVCDDL GFTEFVIKAP YKTPELKLKD KADLVEAFIG ALYVDRGIEH 

       970        980        990       1000       1010       1020 
CRAFIRIVFC PRLKHFIESE KWNDAKSHLQ QWCLAMRDPS SSEPDMPEYR VLGIEGPTNN 

      1030       1040       1050       1060       1070       1080 
RIFKIAVYYK GKRLASAAES NVHKAELRVA ELALANLESM SFSKMKAKNN SWFQNMRRRL 


EQDTSD 

« Hide

Isoform b [UniParc].

Checksum: AEB0EBE694EF1830
Show »

FASTA1,081124,702

References

« Hide 'large scale' references
[1]"A novel type of RNase III family proteins in eukaryotes."
Filippov V., Solovyev V., Filippova M., Gill S.S.
Gene 245:213-221(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 227-1086 (ISOFORM A).
[2]"Genome sequence of the nematode C. elegans: a platform for investigating biology."
The C. elegans sequencing consortium
Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
Strain: Bristol N2.
[3]"Processing of primary microRNAs by the Microprocessor complex."
Denli A.M., Tops B.B.J., Plasterk R.H.A., Ketting R.F., Hannon G.J.
Nature 432:231-235(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF160248 mRNA. Translation: AAD45518.1.
Z81070 Genomic DNA. Translation: CAB03006.3.
Z81070 Genomic DNA. Translation: CAP19331.2.
PIRT21420.
RefSeqNP_001122460.2. NM_001128988.2. [O01326-2]
NP_492599.1. NM_060198.3. [O01326-1]
UniGeneCel.18868.

3D structure databases

ProteinModelPortalO01326.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PaxDbO01326.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaF26E4.10a; F26E4.10a; WBGene00009163. [O01326-1]
GeneID172830.
KEGGcel:CELE_F26E4.10.
UCSCF26E4.10a. c. elegans. [O01326-1]

Organism-specific databases

CTD172830.
WormBaseF26E4.10a; CE29039; WBGene00009163; drsh-1.
F26E4.10b; CE46611; WBGene00009163; drsh-1.

Phylogenomic databases

eggNOGCOG0571.
GeneTreeENSGT00730000111052.
HOGENOMHOG000154167.
InParanoidO01326.
OMAEPILHNE.
OrthoDBEOG773XF7.
PhylomeDBO01326.

Family and domain databases

Gene3D1.10.1520.10. 3 hits.
3.30.160.20. 1 hit.
HAMAPMF_00104. RNase_III.
InterProIPR014720. dsRNA-bd_dom.
IPR011907. RNase_III.
IPR000999. RNase_III_dom.
[Graphical view]
PANTHERPTHR11207. PTHR11207. 1 hit.
PfamPF00035. dsrm. 1 hit.
PF14622. Ribonucleas_3_3. 1 hit.
PF00636. Ribonuclease_3. 1 hit.
[Graphical view]
SMARTSM00358. DSRM. 1 hit.
SM00535. RIBOc. 2 hits.
[Graphical view]
SUPFAMSSF69065. SSF69065. 3 hits.
PROSITEPS50137. DS_RBD. 1 hit.
PS00517. RNASE_3_1. 2 hits.
PS50142. RNASE_3_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio877175.
PROO01326.

Entry information

Entry nameRNC_CAEEL
AccessionPrimary (citable) accession number: O01326
Secondary accession number(s): A8WI03, O01327, Q9U9Q8
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 23, 2002
Last modified: June 11, 2014
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormBase