O01326 (RNC_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease 3
EC=3.1.26.3

Alternative name(s):
Protein drosha
Ribonuclease III
Short name=RNase III

Gene names

Name:	drsh-1
ORF Names:	F26E4.10

Organism

Caenorhabditis elegans [Reference proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	1086 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Executes the initial step of microRNA (miRNA) processing in the nucleus, that is the cleavage of pri-miRNA to release pre-miRNA. Involved in pre-rRNA processing. Cleaves double-strand RNA and does not cleave single-strand RNA. Involved in fertility. Required for the function or synthesis of the let-7 miRNA. Ref.3
Catalytic activity	Endonucleolytic cleavage to 5'-phosphomonoester.
Cofactor	Magnesium or manganese By similarity.
Subcellular location	Nucleus Probable.
Disruption phenotype	Sterility. Ref.3
Sequence similarities	Contains 1 DRBM (double-stranded RNA-binding) domain. Contains 2 RNase III domains.

Ontologies

Keywords
Biological process	Differentiation Gonadal differentiation Ribosome biogenesis rRNA processing
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Domain	Repeat
Ligand	Magnesium Manganese Metal-binding RNA-binding
Molecular function	Developmental protein Endonuclease Hydrolase Nuclease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW gonadal mesoderm development Inferred from electronic annotation. Source: UniProtKB-KW primary miRNA processing Inferred from mutant phenotype Ref.3. Source: UniProtKB rRNA catabolic process Inferred from electronic annotation. Source: InterPro rRNA processing Inferred from electronic annotation. Source: UniProtKB-KW sexual reproduction Inferred from mutant phenotype Ref.3. Source: UniProtKB
Cellular_component	nucleus Inferred by curator Ref.3. Source: UniProtKB
Molecular_function	double-stranded RNA binding Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW ribonuclease III activity Inferred from mutant phenotype Ref.3. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform a (identifier: O01326-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: No experimental confirmation available.

Isoform b (identifier: O01326-2)

The sequence of this isoform differs from the canonical sequence as follows:
     668-669: Missing.
     1072-1074: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1086	1086	Ribonuclease 3		PRO_0000180469
Regions
	Domain	607 – 781	175	RNase III 1
	Domain	833 – 957	125	RNase III 2
	Domain	984 – 1059	76	DRBM
Sites
	Metal binding	873	1	Magnesium or manganese By similarity
	Metal binding	943	1	Magnesium or manganese By similarity
	Metal binding	946	1	Magnesium or manganese By similarity
	Site	939	1	Important for activity By similarity
Natural variations
	Alternative sequence	668 – 669	2	Missing in isoform b.		VSP_043603
	Alternative sequence	1072 – 1074	3	Missing in isoform b.		VSP_034688

Sequences

	Sequence		Length	Mass (Da)
	Isoform a [UniParc]. Last modified January 23, 2002. Version 2. Checksum: 4A478120F88F8FB8	FASTA	1,086	125,334
	10 20 30 40 50 60 MSDEKISMTL NFPKHKRARR KKYQKEYQER HKEEMMQQLG RRFQNQPSTS SAPPDTVEKI 70 80 90 100 110 120 PLPTESTSAL PFGDSPRLTE KDYETNYMID PPVVSTHSAE LIKSNRVVIK AEEAEKYMMI 130 140 150 160 170 180 KAKSTTSKIL QDFQTKILET VKTKRRLQAD VPYIIHPCHS MKGRKTPKQK GGDESFTASD 190 200 210 220 230 240 VSDDSNDSQD EASTSEPTNR QAPEADKTGE VKDEKQTCNR RNQQRKAKRL RNFEEKERQI 250 260 270 280 290 300 TLLKKGIDRK KTHPNGIHPD ISFNEKGLGN EGPECRCPEP IKTCGLKHGY YAGEDKAIDC 310 320 330 340 350 360 KKSNGENLHY YTLRVTPLPS ENQLYRTHMA INGEEFEFEG FSLITHAPLP DCMTRAPICK 370 380 390 400 410 420 YSMDYEFQLV EEFMPDECFD PEDCDMLFEY IFHEIFEMLD FELRPKHIPS DVESCPMIHI 430 440 450 460 470 480 MPRFVQTKDD LVQLWSSKTV LAYFTSKGSS EIMSPEDVNR LCDAQIDQFT RNTSKHKQSI 490 500 510 520 530 540 VLNTKFKPSA IRADWFERDE EKKEVYVVHN AIRAQTYTAI SLPRIAFLEK TLNKMIQEKQ 550 560 570 580 590 600 SSGVYNKDFE KTKNELEHLK RENRSARNLK LREPVAGFIE TGLKPDVAAH VVMTILACHH 610 620 630 640 650 660 IRYNFSLDVF EEVIEYKFND RRVIELALMH SSFKSHYGTP IDHVKNMITN CGYRRKYGAE 670 680 690 700 710 720 DKREKKRVAG IMSLFNIMKG TSGGEPILHN ERLEYLGDAV VELIVSHHLY FMLTHHFEGG 730 740 750 760 770 780 LATYRTALVQ NRNLATLAKN CRIDEMLQYS HGADLINVAE FKHALANAFE AVMAAIYLDG 790 800 810 820 830 840 GLAPCDVIFS KAMYGHQPVL KEKWDHINEH ELKREDPQGD RDLSFITPTL STFHALEERL 850 860 870 880 890 900 GIQFNNIRLL AKAFTRRNIP NNDLTKGHNQ RLEWLGDSVL QLIVSDFLYR RFPYHHEGHM 910 920 930 940 950 960 SLLRTSLVSN QTQAVVCDDL GFTEFVIKAP YKTPELKLKD KADLVEAFIG ALYVDRGIEH 970 980 990 1000 1010 1020 CRAFIRIVFC PRLKHFIESE KWNDAKSHLQ QWCLAMRDPS SSEPDMPEYR VLGIEGPTNN 1030 1040 1050 1060 1070 1080 RIFKIAVYYK GKRLASAAES NVHKAELRVA ELALANLESM SFSKMKAKNN SWFQNMRRRL EQDTSD
	Isoform b [UniParc]. Checksum: AEB0EBE694EF1830	FASTA	1,081	124,702
	10 20 30 40 50 60 MSDEKISMTL NFPKHKRARR KKYQKEYQER HKEEMMQQLG RRFQNQPSTS SAPPDTVEKI 70 80 90 100 110 120 PLPTESTSAL PFGDSPRLTE KDYETNYMID PPVVSTHSAE LIKSNRVVIK AEEAEKYMMI 130 140 150 160 170 180 KAKSTTSKIL QDFQTKILET VKTKRRLQAD VPYIIHPCHS MKGRKTPKQK GGDESFTASD 190 200 210 220 230 240 VSDDSNDSQD EASTSEPTNR QAPEADKTGE VKDEKQTCNR RNQQRKAKRL RNFEEKERQI 250 260 270 280 290 300 TLLKKGIDRK KTHPNGIHPD ISFNEKGLGN EGPECRCPEP IKTCGLKHGY YAGEDKAIDC 310 320 330 340 350 360 KKSNGENLHY YTLRVTPLPS ENQLYRTHMA INGEEFEFEG FSLITHAPLP DCMTRAPICK 370 380 390 400 410 420 YSMDYEFQLV EEFMPDECFD PEDCDMLFEY IFHEIFEMLD FELRPKHIPS DVESCPMIHI 430 440 450 460 470 480 MPRFVQTKDD LVQLWSSKTV LAYFTSKGSS EIMSPEDVNR LCDAQIDQFT RNTSKHKQSI 490 500 510 520 530 540 VLNTKFKPSA IRADWFERDE EKKEVYVVHN AIRAQTYTAI SLPRIAFLEK TLNKMIQEKQ 550 560 570 580 590 600 SSGVYNKDFE KTKNELEHLK RENRSARNLK LREPVAGFIE TGLKPDVAAH VVMTILACHH 610 620 630 640 650 660 IRYNFSLDVF EEVIEYKFND RRVIELALMH SSFKSHYGTP IDHVKNMITN CGYRRKYGAE 670 680 690 700 710 720 DKREKKRGIM SLFNIMKGTS GGEPILHNER LEYLGDAVVE LIVSHHLYFM LTHHFEGGLA 730 740 750 760 770 780 TYRTALVQNR NLATLAKNCR IDEMLQYSHG ADLINVAEFK HALANAFEAV MAAIYLDGGL 790 800 810 820 830 840 APCDVIFSKA MYGHQPVLKE KWDHINEHEL KREDPQGDRD LSFITPTLST FHALEERLGI 850 860 870 880 890 900 QFNNIRLLAK AFTRRNIPNN DLTKGHNQRL EWLGDSVLQL IVSDFLYRRF PYHHEGHMSL 910 920 930 940 950 960 LRTSLVSNQT QAVVCDDLGF TEFVIKAPYK TPELKLKDKA DLVEAFIGAL YVDRGIEHCR 970 980 990 1000 1010 1020 AFIRIVFCPR LKHFIESEKW NDAKSHLQQW CLAMRDPSSS EPDMPEYRVL GIEGPTNNRI 1030 1040 1050 1060 1070 1080 FKIAVYYKGK RLASAAESNV HKAELRVAEL ALANLESMSF SKMKAKNNSN MRRRLEQDTS D

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A novel type of RNase III family proteins in eukaryotes." Filippov V., Solovyev V., Filippova M., Gill S.S. Gene 245:213-221(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 227-1086 (ISOFORM A).
[2]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING. Strain: Bristol N2.
[3]	"Processing of primary microRNAs by the Microprocessor complex." Denli A.M., Tops B.B.J., Plasterk R.H.A., Ketting R.F., Hannon G.J. Nature 432:231-235(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF160248 mRNA. Translation: AAD45518.1. Z81070 Genomic DNA. Translation: CAB03006.3. Z81070 Genomic DNA. Translation: CAP19331.2.
PIR	T21420.
RefSeq	NP_001122460.2. NM_001128988.2. NP_492599.1. NM_060198.3.
3D structure databases
ProteinModelPortal	O01326.
SMR	O01326. Positions 610-783, 836-1065.
ModBase	Search...
Proteomic databases
PaxDb	O01326.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	F26E4.10a; F26E4.10a; F26E4.10.
GeneID	172830.
KEGG	cel:CELE_F26E4.10.
UCSC	F26E4.10a. c. elegans.
Organism-specific databases
CTD	172830.
WormBase	F26E4.10a; CE29039; WBGene00009163; drsh-1. F26E4.10b; CE46611; WBGene00009163; drsh-1.
Phylogenomic databases
eggNOG	COG0571.
GeneTree	ENSGT00690000102147.
HOGENOM	HOG000154167.
InParanoid	O01326.
OMA	RFHKSLD.
Family and domain databases
Gene3D	1.10.1520.10. 3 hits. 3.30.160.20. 1 hit.
InterPro	IPR001159. Ds-RNA-bd. IPR014720. dsRNA-bd-like_dom. IPR011907. RNase_III. IPR000999. RNase_III_dom. [Graphical view]
PANTHER	PTHR11207. PTHR11207. 1 hit.
Pfam	PF00035. dsrm. 1 hit. PF00636. Ribonuclease_3. 2 hits. [Graphical view]
SMART	SM00358. DSRM. 1 hit. SM00535. RIBOc. 2 hits. [Graphical view]
SUPFAM	SSF69065. RNase_III. 2 hits.
PROSITE	PS50137. DS_RBD. 1 hit. PS00517. RNASE_3_1. 2 hits. PS50142. RNASE_3_2. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	877175.

Entry information

Entry name

RNC_CAEEL

Accession

Primary (citable) accession number: O01326
Secondary accession number(s): A8WI03, O01327, Q9U9Q8

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	January 23, 2002
Last modified:	May 1, 2013
This is version 97 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project