SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

O01326

- RNC_CAEEL

UniProt

O01326 - RNC_CAEEL

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Ribonuclease 3
Gene
drsh-1, F26E4.10
Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Executes the initial step of microRNA (miRNA) processing in the nucleus, that is the cleavage of pri-miRNA to release pre-miRNA. Involved in pre-rRNA processing. Cleaves double-strand RNA and does not cleave single-strand RNA. Involved in fertility. Required for the function or synthesis of the let-7 miRNA.1 Publication

Catalytic activityi

Endonucleolytic cleavage to 5'-phosphomonoester.UniRule annotation

Cofactori

Magnesium or manganese By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi873 – 8731Magnesium or manganese By similarity
Sitei939 – 9391Important for activity By similarity
Metal bindingi943 – 9431Magnesium or manganese By similarity
Metal bindingi946 – 9461Magnesium or manganese By similarity

GO - Molecular functioni

  1. RNA binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. ribonuclease III activity Source: UniProtKB
Complete GO annotation...

GO - Biological processi

  1. RNA phosphodiester bond hydrolysis Source: GOC
  2. RNA phosphodiester bond hydrolysis, endonucleolytic Source: GOC
  3. cell differentiation Source: UniProtKB-KW
  4. gonadal mesoderm development Source: UniProtKB-KW
  5. primary miRNA processing Source: UniProtKB
  6. rRNA catabolic process Source: InterPro
  7. rRNA processing Source: UniProtKB-KW
  8. sexual reproduction Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Differentiation, Gonadal differentiation, Ribosome biogenesis, rRNA processing

Keywords - Ligandi

Magnesium, Manganese, Metal-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease 3 (EC:3.1.26.3)
Alternative name(s):
Protein drosha
Ribonuclease III
Short name:
RNase III
Gene namesi
Name:drsh-1
ORF Names:F26E4.10
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940: Chromosome I

Organism-specific databases

WormBaseiF26E4.10a; CE29039; WBGene00009163; drsh-1.
F26E4.10b; CE46611; WBGene00009163; drsh-1.

Subcellular locationi

Nucleus Inferred UniRule annotation

GO - Cellular componenti

  1. microprocessor complex Source: WormBase
  2. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Sterility.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10861086Ribonuclease 3UniRule annotation
PRO_0000180469Add
BLAST

Proteomic databases

PaxDbiO01326.

Structurei

3D structure databases

ProteinModelPortaliO01326.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini607 – 781175RNase III 1
Add
BLAST
Domaini833 – 957125RNase III 2
Add
BLAST
Domaini984 – 105976DRBM
Add
BLAST

Sequence similaritiesi

Contains 2 RNase III domains.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG0571.
GeneTreeiENSGT00730000111052.
HOGENOMiHOG000154167.
InParanoidiO01326.
OMAiEPILHNE.
OrthoDBiEOG773XF7.
PhylomeDBiO01326.

Family and domain databases

Gene3Di1.10.1520.10. 3 hits.
3.30.160.20. 1 hit.
HAMAPiMF_00104. RNase_III.
InterProiIPR014720. dsRNA-bd_dom.
IPR011907. RNase_III.
IPR000999. RNase_III_dom.
[Graphical view]
PANTHERiPTHR11207. PTHR11207. 1 hit.
PfamiPF00035. dsrm. 1 hit.
PF14622. Ribonucleas_3_3. 1 hit.
PF00636. Ribonuclease_3. 1 hit.
[Graphical view]
SMARTiSM00358. DSRM. 1 hit.
SM00535. RIBOc. 2 hits.
[Graphical view]
SUPFAMiSSF69065. SSF69065. 3 hits.
PROSITEiPS50137. DS_RBD. 1 hit.
PS00517. RNASE_3_1. 2 hits.
PS50142. RNASE_3_2. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform a (identifier: O01326-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MSDEKISMTL NFPKHKRARR KKYQKEYQER HKEEMMQQLG RRFQNQPSTS     50
SAPPDTVEKI PLPTESTSAL PFGDSPRLTE KDYETNYMID PPVVSTHSAE 100
LIKSNRVVIK AEEAEKYMMI KAKSTTSKIL QDFQTKILET VKTKRRLQAD 150
VPYIIHPCHS MKGRKTPKQK GGDESFTASD VSDDSNDSQD EASTSEPTNR 200
QAPEADKTGE VKDEKQTCNR RNQQRKAKRL RNFEEKERQI TLLKKGIDRK 250
KTHPNGIHPD ISFNEKGLGN EGPECRCPEP IKTCGLKHGY YAGEDKAIDC 300
KKSNGENLHY YTLRVTPLPS ENQLYRTHMA INGEEFEFEG FSLITHAPLP 350
DCMTRAPICK YSMDYEFQLV EEFMPDECFD PEDCDMLFEY IFHEIFEMLD 400
FELRPKHIPS DVESCPMIHI MPRFVQTKDD LVQLWSSKTV LAYFTSKGSS 450
EIMSPEDVNR LCDAQIDQFT RNTSKHKQSI VLNTKFKPSA IRADWFERDE 500
EKKEVYVVHN AIRAQTYTAI SLPRIAFLEK TLNKMIQEKQ SSGVYNKDFE 550
KTKNELEHLK RENRSARNLK LREPVAGFIE TGLKPDVAAH VVMTILACHH 600
IRYNFSLDVF EEVIEYKFND RRVIELALMH SSFKSHYGTP IDHVKNMITN 650
CGYRRKYGAE DKREKKRVAG IMSLFNIMKG TSGGEPILHN ERLEYLGDAV 700
VELIVSHHLY FMLTHHFEGG LATYRTALVQ NRNLATLAKN CRIDEMLQYS 750
HGADLINVAE FKHALANAFE AVMAAIYLDG GLAPCDVIFS KAMYGHQPVL 800
KEKWDHINEH ELKREDPQGD RDLSFITPTL STFHALEERL GIQFNNIRLL 850
AKAFTRRNIP NNDLTKGHNQ RLEWLGDSVL QLIVSDFLYR RFPYHHEGHM 900
SLLRTSLVSN QTQAVVCDDL GFTEFVIKAP YKTPELKLKD KADLVEAFIG 950
ALYVDRGIEH CRAFIRIVFC PRLKHFIESE KWNDAKSHLQ QWCLAMRDPS 1000
SSEPDMPEYR VLGIEGPTNN RIFKIAVYYK GKRLASAAES NVHKAELRVA 1050
ELALANLESM SFSKMKAKNN SWFQNMRRRL EQDTSD 1086

Note: No experimental confirmation available.

Length:1,086
Mass (Da):125,334
Last modified:January 23, 2002 - v2
Checksum:i4A478120F88F8FB8
GO
Isoform b (identifier: O01326-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     668-669: Missing.
     1072-1074: Missing.

Show »
Length:1,081
Mass (Da):124,702
Checksum:iAEB0EBE694EF1830
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei668 – 6692Missing in isoform b.
VSP_043603
Alternative sequencei1072 – 10743Missing in isoform b.
VSP_034688

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF160248 mRNA. Translation: AAD45518.1.
Z81070 Genomic DNA. Translation: CAB03006.3.
Z81070 Genomic DNA. Translation: CAP19331.2.
PIRiT21420.
RefSeqiNP_001122460.2. NM_001128988.2. [O01326-2]
NP_492599.1. NM_060198.3. [O01326-1]
UniGeneiCel.18868.

Genome annotation databases

EnsemblMetazoaiF26E4.10a; F26E4.10a; WBGene00009163. [O01326-1]
GeneIDi172830.
KEGGicel:CELE_F26E4.10.
UCSCiF26E4.10a. c. elegans. [O01326-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF160248 mRNA. Translation: AAD45518.1 .
Z81070 Genomic DNA. Translation: CAB03006.3 .
Z81070 Genomic DNA. Translation: CAP19331.2 .
PIRi T21420.
RefSeqi NP_001122460.2. NM_001128988.2. [O01326-2 ]
NP_492599.1. NM_060198.3. [O01326-1 ]
UniGenei Cel.18868.

3D structure databases

ProteinModelPortali O01326.
ModBasei Search...

Proteomic databases

PaxDbi O01326.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai F26E4.10a ; F26E4.10a ; WBGene00009163 . [O01326-1 ]
GeneIDi 172830.
KEGGi cel:CELE_F26E4.10.
UCSCi F26E4.10a. c. elegans. [O01326-1 ]

Organism-specific databases

CTDi 172830.
WormBasei F26E4.10a ; CE29039 ; WBGene00009163 ; drsh-1.
F26E4.10b ; CE46611 ; WBGene00009163 ; drsh-1.

Phylogenomic databases

eggNOGi COG0571.
GeneTreei ENSGT00730000111052.
HOGENOMi HOG000154167.
InParanoidi O01326.
OMAi EPILHNE.
OrthoDBi EOG773XF7.
PhylomeDBi O01326.

Miscellaneous databases

NextBioi 877175.
PROi O01326.

Family and domain databases

Gene3Di 1.10.1520.10. 3 hits.
3.30.160.20. 1 hit.
HAMAPi MF_00104. RNase_III.
InterProi IPR014720. dsRNA-bd_dom.
IPR011907. RNase_III.
IPR000999. RNase_III_dom.
[Graphical view ]
PANTHERi PTHR11207. PTHR11207. 1 hit.
Pfami PF00035. dsrm. 1 hit.
PF14622. Ribonucleas_3_3. 1 hit.
PF00636. Ribonuclease_3. 1 hit.
[Graphical view ]
SMARTi SM00358. DSRM. 1 hit.
SM00535. RIBOc. 2 hits.
[Graphical view ]
SUPFAMi SSF69065. SSF69065. 3 hits.
PROSITEi PS50137. DS_RBD. 1 hit.
PS00517. RNASE_3_1. 2 hits.
PS50142. RNASE_3_2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A novel type of RNase III family proteins in eukaryotes."
    Filippov V., Solovyev V., Filippova M., Gill S.S.
    Gene 245:213-221(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 227-1086 (ISOFORM A).
  2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
    Strain: Bristol N2.
  3. "Processing of primary microRNAs by the Microprocessor complex."
    Denli A.M., Tops B.B.J., Plasterk R.H.A., Ketting R.F., Hannon G.J.
    Nature 432:231-235(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiRNC_CAEEL
AccessioniPrimary (citable) accession number: O01326
Secondary accession number(s): A8WI03, O01327, Q9U9Q8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 23, 2002
Last modified: June 11, 2014
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi