Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

O00910

- STATA_DICDI

UniProt

O00910 - STATA_DICDI

Protein

Signal transducer and activator of transcription A

Gene

dstA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 94 (01 Oct 2014)
      Sequence version 1 (01 Jul 1997)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Transcription factor that binds to 5'-TTGAATTGA-3' elements in the promoter region of target genes. Functions as repressor of the ecmB gene. Regulates the differentiation of prestalk cells during development.4 Publications

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei380 – 3801Interaction with DNABy similarity
    Sitei577 – 5771Interaction with DNABy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi443 – 48745Add
    BLAST

    GO - Molecular functioni

    1. calcium ion binding Source: InterPro
    2. DNA binding Source: dictyBase
    3. protein homodimerization activity Source: dictyBase
    4. sequence-specific DNA binding transcription factor activity Source: InterPro
    5. signal transducer activity Source: InterPro

    GO - Biological processi

    1. culmination involved in sorocarp development Source: dictyBase
    2. negative regulation of transcription, DNA-templated Source: dictyBase
    3. positive regulation of gene expression Source: dictyBase
    4. regulation of gene expression Source: dictyBase
    5. sorocarp development Source: dictyBase
    6. sorocarp stalk cell differentiation Source: dictyBase
    7. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Repressor

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Signal transducer and activator of transcription A
    Alternative name(s):
    Dd-STATa
    STAT5 homolog A
    Gene namesi
    Name:dstA
    Synonyms:stat5, statA
    ORF Names:DDB_G0281381
    OrganismiDictyostelium discoideum (Slime mold)
    Taxonomic identifieri44689 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
    ProteomesiUP000002195: Chromosome 3, UP000002195: Unassembled WGS sequence

    Organism-specific databases

    dictyBaseiDDB_G0281381. dstA.

    Subcellular locationi

    Cytoplasm. Nucleus
    Note: Cytoplasmic in growing cells. Translocated into the nucleus in response to cAMP-induced tyrosine phosphorylation. Nuclear at the tight mound stage and in the upper, prestalk region of tipped aggregates and in cells at the tip of the slug. Subject to crm1-dependent nuclear export.

    GO - Cellular componenti

    1. cytosol Source: dictyBase
    2. nucleus Source: dictyBase

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Disruption phenotypei

    Cells are hypersensitive to the chlorinated hexaphenone DIF. They form slugs, but there is little or no stalk cell differentiation. After several days of developmental arrest very small spore masses appear that are supported by columns of apparently undifferentiated cells.1 Publication

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi443 – 4431K → D: Loss of DNA binding. 1 Publication
    Mutagenesisi449 – 4502RK → DD: About 3-fold reduced DNA binding.
    Mutagenesisi484 – 4841N → A: Loss of DNA binding. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 707707Signal transducer and activator of transcription APRO_0000328082Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei702 – 7021Phosphotyrosine1 Publication

    Post-translational modificationi

    Tyrosine phosphorylated in response to cAMP. Not tyrosine phosphorylated in growing cells. Tyrosine phosphorylation is first detected at the tight mound stage, continues throughout the slug stage and early culmination, and starts to decrease at mid-culmination. Barely detectable in fruiting bodies.1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiO00910.

    Expressioni

    Developmental stagei

    Constitutively expressed with a slight increase during the tight mound stage (at protein level). Detected at very low levels in growing cells and aggregates up to the loose mound stage. Highly expressed in tipped aggregates and in the Mexican hat stage. Expressed at lower levels in early and late culminants and in fruiting bodies.2 Publications

    Interactioni

    Subunit structurei

    Monomer, in the absence of tyrosine phosphorylation. Homodimer, or heterodimer with another family member, when tyrosine phosphorylated.2 Publications

    Protein-protein interaction databases

    STRINGi44689.DDB_0215388.

    Structurei

    Secondary structure

    1
    707
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi245 – 27430
    Helixi282 – 31534
    Helixi320 – 35132
    Beta strandi363 – 3686
    Beta strandi374 – 3763
    Turni383 – 3853
    Beta strandi386 – 3916
    Beta strandi398 – 40912
    Turni433 – 4364
    Beta strandi437 – 4393
    Beta strandi453 – 46311
    Beta strandi468 – 4725
    Beta strandi479 – 4846
    Turni485 – 4873
    Helixi488 – 50114
    Beta strandi505 – 5084
    Helixi509 – 52315
    Beta strandi528 – 5303
    Helixi537 – 54610
    Turni547 – 5504
    Beta strandi552 – 5554
    Helixi556 – 57520
    Helixi579 – 5846
    Helixi594 – 5996
    Turni601 – 6033
    Beta strandi609 – 6135
    Beta strandi615 – 6173
    Beta strandi621 – 6266
    Beta strandi628 – 6314
    Beta strandi634 – 6385
    Helixi641 – 6433
    Turni646 – 6483
    Helixi651 – 6555
    Beta strandi663 – 6697
    Beta strandi675 – 6795
    Helixi682 – 6854
    Helixi687 – 6893

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1UURX-ray2.70A235-707[»]
    1UUSX-ray2.80A235-707[»]
    ProteinModelPortaliO00910.
    SMRiO00910. Positions 239-707.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO00910.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini583 – 686104SH2PROSITE-ProRule annotationAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili242 – 3561151 PublicationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi90 – 11122Poly-GlnAdd
    BLAST
    Compositional biasi158 – 21356Poly-AsnAdd
    BLAST
    Compositional biasi214 – 23623Poly-GlnAdd
    BLAST

    Sequence similaritiesi

    Belongs to the transcription factor STAT family.Curated
    Contains 1 SH2 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil, SH2 domain

    Phylogenomic databases

    eggNOGiNOG245085.
    OMAiMNTEMSA.
    PhylomeDBiO00910.

    Family and domain databases

    Gene3Di1.10.238.10. 1 hit.
    1.20.58.240. 1 hit.
    2.60.40.340. 1 hit.
    3.30.505.10. 1 hit.
    InterProiIPR011992. EF-hand-dom_pair.
    IPR008967. p53-like_TF_DNA-bd.
    IPR011539. RHD.
    IPR000980. SH2.
    IPR001217. STAT.
    IPR015988. STAT_TF_coiled-coil.
    IPR015347. STAT_TF_homologue_coiled-coil.
    [Graphical view]
    PANTHERiPTHR11801. PTHR11801. 1 hit.
    PfamiPF09267. Dict-STAT-coil. 1 hit.
    PF00017. SH2. 1 hit.
    [Graphical view]
    SMARTiSM00252. SH2. 1 hit.
    [Graphical view]
    SUPFAMiSSF47655. SSF47655. 1 hit.
    SSF49417. SSF49417. 1 hit.
    SSF55550. SSF55550. 1 hit.
    PROSITEiPS50001. SH2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O00910-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSSAEFSMDD FEDTFDSNAT ISTKDLFEGS DRLPLNQSIN TTIQNLYLPN    50
    GGFAIGDQSQ QQYYQAMPPL NQSDQFNLGR SNNLTPRTNQ LQQLQQQQQQ 100
    QQQPQQQQQQ QTYGTQSPIH MSQTPSSPLS SPLPSPTPFS RQQSYNNNNS 150
    NNTSSSQNYN NNNININNNN NNNNTNNNNN NNNGNNSNGN NGNNNNNNNN 200
    NNNNNTNNNN NNNQQQQQQQ QQQQQQQQQQ QQQQQQGNPN LSSPQPILDT 250
    IYKLLSEQEQ TLVQMIHEQS LLLNRLPPTL DENSLAPLKS LSQKQITLSG 300
    QMNTEMSALD ATKKGMILEP TDLAKLFALK QDLQIQFKQL SLLHNEIQSI 350
    LNPQHSAPKP NVALVLKSQP FPVVISKGKQ LGENQLVVLV LTGARSNFHI 400
    NGPVKATMIC DSHPTNKNNP TTPLEMDSQP IYPATLTAHF PLKFLAGTRK 450
    CSVNLKFGVN IRDLDNVTTT VESDASNPFV VITNECQWEG SAGVLLKKDA 500
    FDGQLEITWA QFINTLQRHF LIATKQDPVR PKRPLSSYDL KYIQTHFFGN 550
    RSIIHQQDFD KFWVWFGKSM QTLRYQRHIS TLWQEGIIYG YMGRQEVNDA 600
    LQNQDPGTFI IRFSERNPGQ FGIAYIGVEM PARIKHYLVQ PNDTAAAKKT 650
    FPDFLSEHSQ FVNLLQWTKD TNGAPRFLKL HKDTALGSFA PKRTAPVPVG 700
    GYEPLNS 707
    Length:707
    Mass (Da):79,792
    Last modified:July 1, 1997 - v1
    Checksum:i9803388BD324D7B6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y13097 mRNA. Translation: CAA73551.1.
    AAFI02000041 Genomic DNA. Translation: EAL66672.1.
    RefSeqiXP_640661.1. XM_635569.1.

    Genome annotation databases

    EnsemblProtistsiDDB0215388; DDB0215388; DDB_G0281381.
    GeneIDi8623045.
    KEGGiddi:DDB_G0281381.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y13097 mRNA. Translation: CAA73551.1 .
    AAFI02000041 Genomic DNA. Translation: EAL66672.1 .
    RefSeqi XP_640661.1. XM_635569.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1UUR X-ray 2.70 A 235-707 [» ]
    1UUS X-ray 2.80 A 235-707 [» ]
    ProteinModelPortali O00910.
    SMRi O00910. Positions 239-707.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 44689.DDB_0215388.

    Proteomic databases

    PRIDEi O00910.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi DDB0215388 ; DDB0215388 ; DDB_G0281381 .
    GeneIDi 8623045.
    KEGGi ddi:DDB_G0281381.

    Organism-specific databases

    dictyBasei DDB_G0281381. dstA.

    Phylogenomic databases

    eggNOGi NOG245085.
    OMAi MNTEMSA.
    PhylomeDBi O00910.

    Miscellaneous databases

    EvolutionaryTracei O00910.

    Family and domain databases

    Gene3Di 1.10.238.10. 1 hit.
    1.20.58.240. 1 hit.
    2.60.40.340. 1 hit.
    3.30.505.10. 1 hit.
    InterProi IPR011992. EF-hand-dom_pair.
    IPR008967. p53-like_TF_DNA-bd.
    IPR011539. RHD.
    IPR000980. SH2.
    IPR001217. STAT.
    IPR015988. STAT_TF_coiled-coil.
    IPR015347. STAT_TF_homologue_coiled-coil.
    [Graphical view ]
    PANTHERi PTHR11801. PTHR11801. 1 hit.
    Pfami PF09267. Dict-STAT-coil. 1 hit.
    PF00017. SH2. 1 hit.
    [Graphical view ]
    SMARTi SM00252. SH2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47655. SSF47655. 1 hit.
    SSF49417. SSF49417. 1 hit.
    SSF55550. SSF55550. 1 hit.
    PROSITEi PS50001. SH2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "SH2 signaling in a lower eukaryote: a STAT protein that regulates stalk cell differentiation in dictyostelium."
      Kawata T., Shevchenko A., Fukuzawa M., Jermyn K.A., Totty N.F., Zhukovskaya N.V., Sterling A.E., Mann M., Williams J.G.
      Cell 89:909-916(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, FUNCTION, SUBUNIT, DEVELOPMENTAL STAGE, TYROSINE PHOSPHORYLATION, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: AX2.
    2. "The genome of the social amoeba Dictyostelium discoideum."
      Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
      , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
      Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.
    3. "Developmentally and spatially regulated activation of a Dictyostelium STAT protein by a serpentine receptor."
      Araki T., Gamper M., Early A., Fukuzawa M., Abe T., Kawata T., Kim E., Firtel R.A., Williams J.G.
      EMBO J. 17:4018-4028(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION AT TYR-702, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.
    4. "The phosphorylated C-terminus of cAR1 plays a role in cell-type-specific gene expression and STATa tyrosine phosphorylation."
      Briscoe C., Moniakis J., Kim J.-Y., Brown J.M., Hereld D., Devreotes P.N., Firtel R.A.
      Dev. Biol. 233:225-236(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: CYCLIC AMP-INDUCED PHOSPHORYLATION.
    5. "Evidence that the Dictyostelium Dd-STATa protein is a repressor that regulates commitment to stalk cell differentiation and is also required for efficient chemotaxis."
      Mohanty S., Jermyn K.A., Early A., Kawata T., Aubry L., Ceccarelli A., Schaap P., Williams J.G., Firtel R.A.
      Development 126:3391-3405(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE.
    6. "Protein tyrosine phosphatase PTP1 negatively regulates Dictyostelium STATa and is required for proper cell-type proportioning."
      Early A., Gamper M., Moniakis J., Kim E., Hunter T., Williams J.G., Firtel R.A.
      Dev. Biol. 232:233-245(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, FUNCTION, MODULATION OF TYROSINE PHOSPHORYLATION.
    7. "Structure of an activated Dictyostelium STAT in its DNA-unbound form."
      Soler-Lopez M., Petosa C., Fukuzawa M., Ravelli R., Williams J.G., Mueller C.W.
      Mol. Cell 13:791-804(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 235-707 OF TYROSINE PHOSPHORYLATED HOMODIMER, COILED-COIL DOMAIN, FUNCTION, SUBUNIT, MUTAGENESIS OF LYS-443; 449-ARG-LYS-450 AND ASN-484.

    Entry informationi

    Entry nameiSTATA_DICDI
    AccessioniPrimary (citable) accession number: O00910
    Secondary accession number(s): Q54U00
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 8, 2008
    Last sequence update: July 1, 1997
    Last modified: October 1, 2014
    This is version 94 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Dictyostelium discoideum
      Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3