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Protein

Lysosomal acid alpha-glucosidase

Gene
N/A
Organism
Tetrahymena pyriformis
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Essential for the degradation of glygogen to glucose in lysosomes. Has both alpha-1,4 and alpha-1,6-glucosidase activity.

Catalytic activityi

Hydrolysis of terminal, non-reducing (1->4)-linked alpha-D-glucose residues with release of alpha-D-glucose.

pH dependencei

Optimum pH is 4.0.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei455NucleophilePROSITE-ProRule annotation1
Active sitei458By similarity1
Active sitei585Proton donorBy similarity1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Protein family/group databases

CAZyiGH31. Glycoside Hydrolase Family 31.

Names & Taxonomyi

Protein namesi
Recommended name:
Lysosomal acid alpha-glucosidase (EC:3.2.1.20)
Alternative name(s):
Acid maltase
OrganismiTetrahymena pyriformis
Taxonomic identifieri5908 [NCBI]
Taxonomic lineageiEukaryotaAlveolataCiliophoraIntramacronucleataOligohymenophoreaHymenostomatidaTetrahymeninaTetrahymenidaeTetrahymena

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Lysosome, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 17Sequence analysisAdd BLAST17
PropeptideiPRO_000001857418 – 36Add BLAST19
ChainiPRO_000001857537 – 923Lysosomal acid alpha-glucosidaseAdd BLAST887

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi65N-linked (GlcNAc...)Sequence analysis1
Glycosylationi405N-linked (GlcNAc...)Sequence analysis1
Glycosylationi440N-linked (GlcNAc...)Sequence analysis1
Glycosylationi586N-linked (GlcNAc...)Sequence analysis1
Glycosylationi621N-linked (GlcNAc...)Sequence analysis1
Glycosylationi646N-linked (GlcNAc...)Sequence analysis1
Glycosylationi848N-linked (GlcNAc...)Sequence analysis1
Glycosylationi908N-linked (GlcNAc...)Sequence analysis1
Glycosylationi912N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 31 family.Curated

Keywords - Domaini

Signal

Family and domain databases

InterProiIPR031727. Gal_mutarotase_N.
IPR011013. Gal_mutarotase_SF_dom.
IPR000322. Glyco_hydro_31.
IPR030458. Glyco_hydro_31_AS.
IPR030459. Glyco_hydro_31_CS.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF01055. Glyco_hydro_31. 1 hit.
PF16863. NtCtMGAM_N. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 2 hits.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00129. GLYCOSYL_HYDROL_F31_1. 1 hit.
PS00707. GLYCOSYL_HYDROL_F31_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O00906-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKHQVLLPLL VTTAIIAGSV GVYTHSKPLL GQSQDQVLPP FTPPLQNGHI
60 70 80 90 100
DLQGKYIVST LDQVNATHIN IYANYNGPEA SYAMPKNKLI THILVSIVIN
110 120 130 140 150
DVNQLGIKIT DRTYRHFEVP YSNLFPHDKV FNFPANNQFD ITLPKRGEAF
160 170 180 190 200
YLTIKRKDTG EVVFDTNNQF FVYSDLYHEF TVAMQNEFIY GLGERRNKQF
210 220 230 240 250
LYDSGEYTFL NKDQYESVAD GHPDQQTYGT HPMYLRRENS GNFHVVFLRN
260 270 280 290 300
YNSIQAVYSK GKSLTYKVVG GLLEFKIFLG DKSPETSLKL YHSYVNGFNL
310 320 330 340 350
HPFWAHGFHQ CRWGYKTSEM MTTVWDTFNT NGLPFDTIWS DIDYMKDLTD
360 370 380 390 400
FTIDTSRYDK AQMNTMLDRS VAAGVHWVPI IDAGIALGDV SNERGKELGV
410 420 430 440 450
YQKSNKTGED LIGCVWPGKV NYPDFNHPLS QEFWAEGLMN LTKNYGITPS
460 470 480 490 500
GFWIDMNEFS NFINGEISED QNCIMPGDTT TNPNYLGNSV EDFYTRIPFE
510 520 530 540 550
VGGADHPQQE KTMSYDAPKY NYADAKTVYI PNYELREFDF HNLNGFSEGI
560 570 580 590 600
ATNYALKKMG NKLPFIISRS QIAGSGQFVQ HWTGDNGSQW DFLQYSLGEI
610 620 630 640 650
FNFNMYGIPM TGADICGFAQ NTTAELCARW MQVGAFYPFS RNHNSNDTIP
660 670 680 690 700
QEPYAFPDST YVLDSSKKSL RLRYALLKQY YSHFVSSNGV GTVFRPTFFN
710 720 730 740 750
FPDDASLLTN DQQFMIGDSL LGQPVLVQSA TPARFSHSSY LTFPSSGAFY
760 770 780 790 800
DFVTDVATLN AQRYTNANNG QIKNVKFDDI MPLYIREGYT VFTQLASTAL
810 820 830 840 850
RSRLLDSNFE LHVALAKSGT SYTAKGKFIT IQDYSDDNLI QKCIGANNCS
860 870 880 890 900
FDIQVTGVVN GANLDLTIQI AGESAQTNFE TINVNKIIPY AADLKFAAST
910 920
ATFTISKNGT INASIPLQAA QQE
Length:923
Mass (Da):104,117
Last modified:July 1, 1997 - v1
Checksum:i6294809F43EA54C3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D83384 mRNA. Translation: BAA20462.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D83384 mRNA. Translation: BAA20462.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH31. Glycoside Hydrolase Family 31.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR031727. Gal_mutarotase_N.
IPR011013. Gal_mutarotase_SF_dom.
IPR000322. Glyco_hydro_31.
IPR030458. Glyco_hydro_31_AS.
IPR030459. Glyco_hydro_31_CS.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF01055. Glyco_hydro_31. 1 hit.
PF16863. NtCtMGAM_N. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 2 hits.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00129. GLYCOSYL_HYDROL_F31_1. 1 hit.
PS00707. GLYCOSYL_HYDROL_F31_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAGLU_TETPY
AccessioniPrimary (citable) accession number: O00906
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 1, 1997
Last modified: November 11, 2015
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.