O00906 (AGLU_TETPY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Lysosomal acid alpha-glucosidase EC=3.2.1.20 Alternative name(s): Acid maltase
Organism	Tetrahymena pyriformis
Taxonomic identifier	5908 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Alveolata › Ciliophora › Intramacronucleata › Oligohymenophorea › Hymenostomatida › Tetrahymenina › Tetrahymenidae › Tetrahymena

Protein attributes

Sequence length	923 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Essential for the degradation of glygogen to glucose in lysosomes. Has both alpha-1,4 and alpha-1,6-glucosidase activity.
Catalytic activity	Hydrolysis of terminal, non-reducing (1->4)-linked alpha-D-glucose residues with release of alpha-D-glucose.
Subcellular location	Lysosome. Secreted.
Sequence similarities	Belongs to the glycosyl hydrolase 31 family.
Biophysicochemical properties	pH dependence: Optimum pH is 4.0.

Ontologies

Keywords
Cellular component	Lysosome Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell lysosome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	carbohydrate binding Inferred from electronic annotation. Source: InterPro maltose alpha-glucosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 17	17	Potential
	Propeptide	18 – 36	19			PRO_0000018574
	Chain	37 – 923	887	Lysosomal acid alpha-glucosidase		PRO_0000018575
Sites
	Active site	455	1	Nucleophile By similarity
	Active site	458	1	By similarity
	Active site	585	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	65	1	N-linked (GlcNAc...) Potential
	Glycosylation	405	1	N-linked (GlcNAc...) Potential
	Glycosylation	440	1	N-linked (GlcNAc...) Potential
	Glycosylation	586	1	N-linked (GlcNAc...) Potential
	Glycosylation	621	1	N-linked (GlcNAc...) Potential
	Glycosylation	646	1	N-linked (GlcNAc...) Potential
	Glycosylation	848	1	N-linked (GlcNAc...) Potential
	Glycosylation	908	1	N-linked (GlcNAc...) Potential
	Glycosylation	912	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	O00906 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: 6294809F43EA54C3	FASTA	923	104,117
	10 20 30 40 50 60 MKHQVLLPLL VTTAIIAGSV GVYTHSKPLL GQSQDQVLPP FTPPLQNGHI DLQGKYIVST 70 80 90 100 110 120 LDQVNATHIN IYANYNGPEA SYAMPKNKLI THILVSIVIN DVNQLGIKIT DRTYRHFEVP 130 140 150 160 170 180 YSNLFPHDKV FNFPANNQFD ITLPKRGEAF YLTIKRKDTG EVVFDTNNQF FVYSDLYHEF 190 200 210 220 230 240 TVAMQNEFIY GLGERRNKQF LYDSGEYTFL NKDQYESVAD GHPDQQTYGT HPMYLRRENS 250 260 270 280 290 300 GNFHVVFLRN YNSIQAVYSK GKSLTYKVVG GLLEFKIFLG DKSPETSLKL YHSYVNGFNL 310 320 330 340 350 360 HPFWAHGFHQ CRWGYKTSEM MTTVWDTFNT NGLPFDTIWS DIDYMKDLTD FTIDTSRYDK 370 380 390 400 410 420 AQMNTMLDRS VAAGVHWVPI IDAGIALGDV SNERGKELGV YQKSNKTGED LIGCVWPGKV 430 440 450 460 470 480 NYPDFNHPLS QEFWAEGLMN LTKNYGITPS GFWIDMNEFS NFINGEISED QNCIMPGDTT 490 500 510 520 530 540 TNPNYLGNSV EDFYTRIPFE VGGADHPQQE KTMSYDAPKY NYADAKTVYI PNYELREFDF 550 560 570 580 590 600 HNLNGFSEGI ATNYALKKMG NKLPFIISRS QIAGSGQFVQ HWTGDNGSQW DFLQYSLGEI 610 620 630 640 650 660 FNFNMYGIPM TGADICGFAQ NTTAELCARW MQVGAFYPFS RNHNSNDTIP QEPYAFPDST 670 680 690 700 710 720 YVLDSSKKSL RLRYALLKQY YSHFVSSNGV GTVFRPTFFN FPDDASLLTN DQQFMIGDSL 730 740 750 760 770 780 LGQPVLVQSA TPARFSHSSY LTFPSSGAFY DFVTDVATLN AQRYTNANNG QIKNVKFDDI 790 800 810 820 830 840 MPLYIREGYT VFTQLASTAL RSRLLDSNFE LHVALAKSGT SYTAKGKFIT IQDYSDDNLI 850 860 870 880 890 900 QKCIGANNCS FDIQVTGVVN GANLDLTIQI AGESAQTNFE TINVNKIIPY AADLKFAAST 910 920 ATFTISKNGT INASIPLQAA QQE

References

[1]

"Molecular cloning of a gene encoding acid alpha-glucosidase from Tetrahymena pyriformis." Alam S., Nakashima S., Deyashiki Y., Banno Y., Hara A., Nozawa Y. J. Eukaryot. Microbiol. 43:295-303(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Strain: W.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D83384 mRNA. Translation: BAA20462.1.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GH31. Glycoside Hydrolase Family 31.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR011013. Gal_mutarotase_SF_dom. IPR000322. Glyco_hydro_31. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
PANTHER	PTHR22762. PTHR22762. 1 hit.
Pfam	PF01055. Glyco_hydro_31. 1 hit. [Graphical view]
SUPFAM	SSF74650. Gal_mut_like. 1 hit. SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00129. GLYCOSYL_HYDROL_F31_1. 1 hit. PS00707. GLYCOSYL_HYDROL_F31_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AGLU_TETPY

Accession

Primary (citable) accession number: O00906

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	July 1, 1997
Last modified:	April 3, 2013
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)