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O00906 (AGLU_TETPY) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Lysosomal acid alpha-glucosidase

EC=3.2.1.20
Alternative name(s):
Acid maltase
OrganismTetrahymena pyriformis
Taxonomic identifier5908 [NCBI]
Taxonomic lineageEukaryotaAlveolataCiliophoraIntramacronucleataOligohymenophoreaHymenostomatidaTetrahymeninaTetrahymenidaeTetrahymena

Protein attributes

Sequence length923 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Essential for the degradation of glygogen to glucose in lysosomes. Has both alpha-1,4 and alpha-1,6-glucosidase activity.

Catalytic activity

Hydrolysis of terminal, non-reducing (1->4)-linked alpha-D-glucose residues with release of alpha-D-glucose.

Subcellular location

Lysosome. Secreted.

Sequence similarities

Belongs to the glycosyl hydrolase 31 family.

Biophysicochemical properties

pH dependence:

Optimum pH is 4.0.

Ontologies

Keywords
   Cellular componentLysosome
Secreted
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   PTMGlycoprotein
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

lysosome

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functioncarbohydrate binding

Inferred from electronic annotation. Source: InterPro

maltose alpha-glucosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1717 Potential
Propeptide18 – 3619
PRO_0000018574
Chain37 – 923887Lysosomal acid alpha-glucosidase
PRO_0000018575

Sites

Active site4551Nucleophile By similarity
Active site4581 By similarity
Active site5851Proton donor By similarity

Amino acid modifications

Glycosylation651N-linked (GlcNAc...) Potential
Glycosylation4051N-linked (GlcNAc...) Potential
Glycosylation4401N-linked (GlcNAc...) Potential
Glycosylation5861N-linked (GlcNAc...) Potential
Glycosylation6211N-linked (GlcNAc...) Potential
Glycosylation6461N-linked (GlcNAc...) Potential
Glycosylation8481N-linked (GlcNAc...) Potential
Glycosylation9081N-linked (GlcNAc...) Potential
Glycosylation9121N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
O00906 [UniParc].

Last modified July 1, 1997. Version 1.
Checksum: 6294809F43EA54C3

FASTA923104,117
        10         20         30         40         50         60 
MKHQVLLPLL VTTAIIAGSV GVYTHSKPLL GQSQDQVLPP FTPPLQNGHI DLQGKYIVST 

        70         80         90        100        110        120 
LDQVNATHIN IYANYNGPEA SYAMPKNKLI THILVSIVIN DVNQLGIKIT DRTYRHFEVP 

       130        140        150        160        170        180 
YSNLFPHDKV FNFPANNQFD ITLPKRGEAF YLTIKRKDTG EVVFDTNNQF FVYSDLYHEF 

       190        200        210        220        230        240 
TVAMQNEFIY GLGERRNKQF LYDSGEYTFL NKDQYESVAD GHPDQQTYGT HPMYLRRENS 

       250        260        270        280        290        300 
GNFHVVFLRN YNSIQAVYSK GKSLTYKVVG GLLEFKIFLG DKSPETSLKL YHSYVNGFNL 

       310        320        330        340        350        360 
HPFWAHGFHQ CRWGYKTSEM MTTVWDTFNT NGLPFDTIWS DIDYMKDLTD FTIDTSRYDK 

       370        380        390        400        410        420 
AQMNTMLDRS VAAGVHWVPI IDAGIALGDV SNERGKELGV YQKSNKTGED LIGCVWPGKV 

       430        440        450        460        470        480 
NYPDFNHPLS QEFWAEGLMN LTKNYGITPS GFWIDMNEFS NFINGEISED QNCIMPGDTT 

       490        500        510        520        530        540 
TNPNYLGNSV EDFYTRIPFE VGGADHPQQE KTMSYDAPKY NYADAKTVYI PNYELREFDF 

       550        560        570        580        590        600 
HNLNGFSEGI ATNYALKKMG NKLPFIISRS QIAGSGQFVQ HWTGDNGSQW DFLQYSLGEI 

       610        620        630        640        650        660 
FNFNMYGIPM TGADICGFAQ NTTAELCARW MQVGAFYPFS RNHNSNDTIP QEPYAFPDST 

       670        680        690        700        710        720 
YVLDSSKKSL RLRYALLKQY YSHFVSSNGV GTVFRPTFFN FPDDASLLTN DQQFMIGDSL 

       730        740        750        760        770        780 
LGQPVLVQSA TPARFSHSSY LTFPSSGAFY DFVTDVATLN AQRYTNANNG QIKNVKFDDI 

       790        800        810        820        830        840 
MPLYIREGYT VFTQLASTAL RSRLLDSNFE LHVALAKSGT SYTAKGKFIT IQDYSDDNLI 

       850        860        870        880        890        900 
QKCIGANNCS FDIQVTGVVN GANLDLTIQI AGESAQTNFE TINVNKIIPY AADLKFAAST 

       910        920 
ATFTISKNGT INASIPLQAA QQE 

« Hide

References

[1]"Molecular cloning of a gene encoding acid alpha-glucosidase from Tetrahymena pyriformis."
Alam S., Nakashima S., Deyashiki Y., Banno Y., Hara A., Nozawa Y.
J. Eukaryot. Microbiol. 43:295-303(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
Strain: W.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D83384 mRNA. Translation: BAA20462.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH31. Glycoside Hydrolase Family 31.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR011013. Gal_mutarotase_SF_dom.
IPR000322. Glyco_hydro_31.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF01055. Glyco_hydro_31. 1 hit.
[Graphical view]
SUPFAMSSF51445. SSF51445. 2 hits.
SSF74650. SSF74650. 1 hit.
PROSITEPS00129. GLYCOSYL_HYDROL_F31_1. 1 hit.
PS00707. GLYCOSYL_HYDROL_F31_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameAGLU_TETPY
AccessionPrimary (citable) accession number: O00906
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 1, 1997
Last modified: October 16, 2013
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries