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O00906

- AGLU_TETPY

UniProt

O00906 - AGLU_TETPY

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Protein
Lysosomal acid alpha-glucosidase
Gene
N/A
Organism
Tetrahymena pyriformis
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Essential for the degradation of glygogen to glucose in lysosomes. Has both alpha-1,4 and alpha-1,6-glucosidase activity.

Catalytic activityi

Hydrolysis of terminal, non-reducing (1->4)-linked alpha-D-glucose residues with release of alpha-D-glucose.

pH dependencei

Optimum pH is 4.0.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei455 – 4551Nucleophile By similarity
Active sitei458 – 4581 By similarity
Active sitei585 – 5851Proton donor By similarity

GO - Molecular functioni

  1. carbohydrate binding Source: InterPro
  2. maltose alpha-glucosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Protein family/group databases

CAZyiGH31. Glycoside Hydrolase Family 31.

Names & Taxonomyi

Protein namesi
Recommended name:
Lysosomal acid alpha-glucosidase (EC:3.2.1.20)
Alternative name(s):
Acid maltase
OrganismiTetrahymena pyriformis
Taxonomic identifieri5908 [NCBI]
Taxonomic lineageiEukaryotaAlveolataCiliophoraIntramacronucleataOligohymenophoreaHymenostomatidaTetrahymeninaTetrahymenidaeTetrahymena

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
  2. lysosome Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Lysosome, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1717 Reviewed prediction
Add
BLAST
Propeptidei18 – 3619
PRO_0000018574Add
BLAST
Chaini37 – 923887Lysosomal acid alpha-glucosidase
PRO_0000018575Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi65 – 651N-linked (GlcNAc...) Reviewed prediction
Glycosylationi405 – 4051N-linked (GlcNAc...) Reviewed prediction
Glycosylationi440 – 4401N-linked (GlcNAc...) Reviewed prediction
Glycosylationi586 – 5861N-linked (GlcNAc...) Reviewed prediction
Glycosylationi621 – 6211N-linked (GlcNAc...) Reviewed prediction
Glycosylationi646 – 6461N-linked (GlcNAc...) Reviewed prediction
Glycosylationi848 – 8481N-linked (GlcNAc...) Reviewed prediction
Glycosylationi908 – 9081N-linked (GlcNAc...) Reviewed prediction
Glycosylationi912 – 9121N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Glycoprotein

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

InterProiIPR011013. Gal_mutarotase_SF_dom.
IPR000322. Glyco_hydro_31.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF01055. Glyco_hydro_31. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 2 hits.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00129. GLYCOSYL_HYDROL_F31_1. 1 hit.
PS00707. GLYCOSYL_HYDROL_F31_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O00906-1 [UniParc]FASTAAdd to Basket

« Hide

MKHQVLLPLL VTTAIIAGSV GVYTHSKPLL GQSQDQVLPP FTPPLQNGHI    50
DLQGKYIVST LDQVNATHIN IYANYNGPEA SYAMPKNKLI THILVSIVIN 100
DVNQLGIKIT DRTYRHFEVP YSNLFPHDKV FNFPANNQFD ITLPKRGEAF 150
YLTIKRKDTG EVVFDTNNQF FVYSDLYHEF TVAMQNEFIY GLGERRNKQF 200
LYDSGEYTFL NKDQYESVAD GHPDQQTYGT HPMYLRRENS GNFHVVFLRN 250
YNSIQAVYSK GKSLTYKVVG GLLEFKIFLG DKSPETSLKL YHSYVNGFNL 300
HPFWAHGFHQ CRWGYKTSEM MTTVWDTFNT NGLPFDTIWS DIDYMKDLTD 350
FTIDTSRYDK AQMNTMLDRS VAAGVHWVPI IDAGIALGDV SNERGKELGV 400
YQKSNKTGED LIGCVWPGKV NYPDFNHPLS QEFWAEGLMN LTKNYGITPS 450
GFWIDMNEFS NFINGEISED QNCIMPGDTT TNPNYLGNSV EDFYTRIPFE 500
VGGADHPQQE KTMSYDAPKY NYADAKTVYI PNYELREFDF HNLNGFSEGI 550
ATNYALKKMG NKLPFIISRS QIAGSGQFVQ HWTGDNGSQW DFLQYSLGEI 600
FNFNMYGIPM TGADICGFAQ NTTAELCARW MQVGAFYPFS RNHNSNDTIP 650
QEPYAFPDST YVLDSSKKSL RLRYALLKQY YSHFVSSNGV GTVFRPTFFN 700
FPDDASLLTN DQQFMIGDSL LGQPVLVQSA TPARFSHSSY LTFPSSGAFY 750
DFVTDVATLN AQRYTNANNG QIKNVKFDDI MPLYIREGYT VFTQLASTAL 800
RSRLLDSNFE LHVALAKSGT SYTAKGKFIT IQDYSDDNLI QKCIGANNCS 850
FDIQVTGVVN GANLDLTIQI AGESAQTNFE TINVNKIIPY AADLKFAAST 900
ATFTISKNGT INASIPLQAA QQE 923
Length:923
Mass (Da):104,117
Last modified:July 1, 1997 - v1
Checksum:i6294809F43EA54C3
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D83384 mRNA. Translation: BAA20462.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D83384 mRNA. Translation: BAA20462.1 .

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH31. Glycoside Hydrolase Family 31.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR011013. Gal_mutarotase_SF_dom.
IPR000322. Glyco_hydro_31.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF01055. Glyco_hydro_31. 1 hit.
[Graphical view ]
SUPFAMi SSF51445. SSF51445. 2 hits.
SSF74650. SSF74650. 1 hit.
PROSITEi PS00129. GLYCOSYL_HYDROL_F31_1. 1 hit.
PS00707. GLYCOSYL_HYDROL_F31_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning of a gene encoding acid alpha-glucosidase from Tetrahymena pyriformis."
    Alam S., Nakashima S., Deyashiki Y., Banno Y., Hara A., Nozawa Y.
    J. Eukaryot. Microbiol. 43:295-303(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    Strain: W.

Entry informationi

Entry nameiAGLU_TETPY
AccessioniPrimary (citable) accession number: O00906
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 1, 1997
Last modified: October 16, 2013
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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