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O00906

- AGLU_TETPY

UniProt

O00906 - AGLU_TETPY

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Protein

Lysosomal acid alpha-glucosidase

Gene
N/A
Organism
Tetrahymena pyriformis
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Essential for the degradation of glygogen to glucose in lysosomes. Has both alpha-1,4 and alpha-1,6-glucosidase activity.

Catalytic activityi

Hydrolysis of terminal, non-reducing (1->4)-linked alpha-D-glucose residues with release of alpha-D-glucose.

pH dependencei

Optimum pH is 4.0.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei455 – 4551NucleophilePROSITE-ProRule annotation
Active sitei458 – 4581By similarity
Active sitei585 – 5851Proton donorBy similarity

GO - Molecular functioni

  1. alpha-1,4-glucosidase activity Source: UniProtKB-EC
  2. carbohydrate binding Source: InterPro
  3. maltose alpha-glucosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Protein family/group databases

CAZyiGH31. Glycoside Hydrolase Family 31.

Names & Taxonomyi

Protein namesi
Recommended name:
Lysosomal acid alpha-glucosidase (EC:3.2.1.20)
Alternative name(s):
Acid maltase
OrganismiTetrahymena pyriformis
Taxonomic identifieri5908 [NCBI]
Taxonomic lineageiEukaryotaAlveolataCiliophoraIntramacronucleataOligohymenophoreaHymenostomatidaTetrahymeninaTetrahymenidaeTetrahymena

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
  2. lysosome Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Lysosome, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1717Sequence AnalysisAdd
BLAST
Propeptidei18 – 3619PRO_0000018574Add
BLAST
Chaini37 – 923887Lysosomal acid alpha-glucosidasePRO_0000018575Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi65 – 651N-linked (GlcNAc...)Sequence Analysis
Glycosylationi405 – 4051N-linked (GlcNAc...)Sequence Analysis
Glycosylationi440 – 4401N-linked (GlcNAc...)Sequence Analysis
Glycosylationi586 – 5861N-linked (GlcNAc...)Sequence Analysis
Glycosylationi621 – 6211N-linked (GlcNAc...)Sequence Analysis
Glycosylationi646 – 6461N-linked (GlcNAc...)Sequence Analysis
Glycosylationi848 – 8481N-linked (GlcNAc...)Sequence Analysis
Glycosylationi908 – 9081N-linked (GlcNAc...)Sequence Analysis
Glycosylationi912 – 9121N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 31 family.Curated

Keywords - Domaini

Signal

Family and domain databases

InterProiIPR011013. Gal_mutarotase_SF_dom.
IPR000322. Glyco_hydro_31.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF01055. Glyco_hydro_31. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 2 hits.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00129. GLYCOSYL_HYDROL_F31_1. 1 hit.
PS00707. GLYCOSYL_HYDROL_F31_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O00906-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKHQVLLPLL VTTAIIAGSV GVYTHSKPLL GQSQDQVLPP FTPPLQNGHI
60 70 80 90 100
DLQGKYIVST LDQVNATHIN IYANYNGPEA SYAMPKNKLI THILVSIVIN
110 120 130 140 150
DVNQLGIKIT DRTYRHFEVP YSNLFPHDKV FNFPANNQFD ITLPKRGEAF
160 170 180 190 200
YLTIKRKDTG EVVFDTNNQF FVYSDLYHEF TVAMQNEFIY GLGERRNKQF
210 220 230 240 250
LYDSGEYTFL NKDQYESVAD GHPDQQTYGT HPMYLRRENS GNFHVVFLRN
260 270 280 290 300
YNSIQAVYSK GKSLTYKVVG GLLEFKIFLG DKSPETSLKL YHSYVNGFNL
310 320 330 340 350
HPFWAHGFHQ CRWGYKTSEM MTTVWDTFNT NGLPFDTIWS DIDYMKDLTD
360 370 380 390 400
FTIDTSRYDK AQMNTMLDRS VAAGVHWVPI IDAGIALGDV SNERGKELGV
410 420 430 440 450
YQKSNKTGED LIGCVWPGKV NYPDFNHPLS QEFWAEGLMN LTKNYGITPS
460 470 480 490 500
GFWIDMNEFS NFINGEISED QNCIMPGDTT TNPNYLGNSV EDFYTRIPFE
510 520 530 540 550
VGGADHPQQE KTMSYDAPKY NYADAKTVYI PNYELREFDF HNLNGFSEGI
560 570 580 590 600
ATNYALKKMG NKLPFIISRS QIAGSGQFVQ HWTGDNGSQW DFLQYSLGEI
610 620 630 640 650
FNFNMYGIPM TGADICGFAQ NTTAELCARW MQVGAFYPFS RNHNSNDTIP
660 670 680 690 700
QEPYAFPDST YVLDSSKKSL RLRYALLKQY YSHFVSSNGV GTVFRPTFFN
710 720 730 740 750
FPDDASLLTN DQQFMIGDSL LGQPVLVQSA TPARFSHSSY LTFPSSGAFY
760 770 780 790 800
DFVTDVATLN AQRYTNANNG QIKNVKFDDI MPLYIREGYT VFTQLASTAL
810 820 830 840 850
RSRLLDSNFE LHVALAKSGT SYTAKGKFIT IQDYSDDNLI QKCIGANNCS
860 870 880 890 900
FDIQVTGVVN GANLDLTIQI AGESAQTNFE TINVNKIIPY AADLKFAAST
910 920
ATFTISKNGT INASIPLQAA QQE
Length:923
Mass (Da):104,117
Last modified:July 1, 1997 - v1
Checksum:i6294809F43EA54C3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D83384 mRNA. Translation: BAA20462.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D83384 mRNA. Translation: BAA20462.1 .

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH31. Glycoside Hydrolase Family 31.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR011013. Gal_mutarotase_SF_dom.
IPR000322. Glyco_hydro_31.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF01055. Glyco_hydro_31. 1 hit.
[Graphical view ]
SUPFAMi SSF51445. SSF51445. 2 hits.
SSF74650. SSF74650. 1 hit.
PROSITEi PS00129. GLYCOSYL_HYDROL_F31_1. 1 hit.
PS00707. GLYCOSYL_HYDROL_F31_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning of a gene encoding acid alpha-glucosidase from Tetrahymena pyriformis."
    Alam S., Nakashima S., Deyashiki Y., Banno Y., Hara A., Nozawa Y.
    J. Eukaryot. Microbiol. 43:295-303(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    Strain: W.

Entry informationi

Entry nameiAGLU_TETPY
AccessioniPrimary (citable) accession number: O00906
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 1, 1997
Last modified: October 29, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3