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O00906

- AGLU_TETPY

UniProt

O00906 - AGLU_TETPY

Protein

Lysosomal acid alpha-glucosidase

Gene
N/A
Organism
Tetrahymena pyriformis
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 54 (01 Oct 2014)
      Sequence version 1 (01 Jul 1997)
      Previous versions | rss
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    Functioni

    Essential for the degradation of glygogen to glucose in lysosomes. Has both alpha-1,4 and alpha-1,6-glucosidase activity.

    Catalytic activityi

    Hydrolysis of terminal, non-reducing (1->4)-linked alpha-D-glucose residues with release of alpha-D-glucose.

    pH dependencei

    Optimum pH is 4.0.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei455 – 4551NucleophilePROSITE-ProRule annotation
    Active sitei458 – 4581By similarity
    Active sitei585 – 5851Proton donorBy similarity

    GO - Molecular functioni

    1. carbohydrate binding Source: InterPro
    2. maltose alpha-glucosidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Protein family/group databases

    CAZyiGH31. Glycoside Hydrolase Family 31.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lysosomal acid alpha-glucosidase (EC:3.2.1.20)
    Alternative name(s):
    Acid maltase
    OrganismiTetrahymena pyriformis
    Taxonomic identifieri5908 [NCBI]
    Taxonomic lineageiEukaryotaAlveolataCiliophoraIntramacronucleataOligohymenophoreaHymenostomatidaTetrahymeninaTetrahymenidaeTetrahymena

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell
    2. lysosome Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Lysosome, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1717Sequence AnalysisAdd
    BLAST
    Propeptidei18 – 3619PRO_0000018574Add
    BLAST
    Chaini37 – 923887Lysosomal acid alpha-glucosidasePRO_0000018575Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi65 – 651N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi405 – 4051N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi440 – 4401N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi586 – 5861N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi621 – 6211N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi646 – 6461N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi848 – 8481N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi908 – 9081N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi912 – 9121N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 31 family.Curated

    Keywords - Domaini

    Signal

    Family and domain databases

    InterProiIPR011013. Gal_mutarotase_SF_dom.
    IPR000322. Glyco_hydro_31.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF01055. Glyco_hydro_31. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 2 hits.
    SSF74650. SSF74650. 1 hit.
    PROSITEiPS00129. GLYCOSYL_HYDROL_F31_1. 1 hit.
    PS00707. GLYCOSYL_HYDROL_F31_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O00906-1 [UniParc]FASTAAdd to Basket

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    MKHQVLLPLL VTTAIIAGSV GVYTHSKPLL GQSQDQVLPP FTPPLQNGHI    50
    DLQGKYIVST LDQVNATHIN IYANYNGPEA SYAMPKNKLI THILVSIVIN 100
    DVNQLGIKIT DRTYRHFEVP YSNLFPHDKV FNFPANNQFD ITLPKRGEAF 150
    YLTIKRKDTG EVVFDTNNQF FVYSDLYHEF TVAMQNEFIY GLGERRNKQF 200
    LYDSGEYTFL NKDQYESVAD GHPDQQTYGT HPMYLRRENS GNFHVVFLRN 250
    YNSIQAVYSK GKSLTYKVVG GLLEFKIFLG DKSPETSLKL YHSYVNGFNL 300
    HPFWAHGFHQ CRWGYKTSEM MTTVWDTFNT NGLPFDTIWS DIDYMKDLTD 350
    FTIDTSRYDK AQMNTMLDRS VAAGVHWVPI IDAGIALGDV SNERGKELGV 400
    YQKSNKTGED LIGCVWPGKV NYPDFNHPLS QEFWAEGLMN LTKNYGITPS 450
    GFWIDMNEFS NFINGEISED QNCIMPGDTT TNPNYLGNSV EDFYTRIPFE 500
    VGGADHPQQE KTMSYDAPKY NYADAKTVYI PNYELREFDF HNLNGFSEGI 550
    ATNYALKKMG NKLPFIISRS QIAGSGQFVQ HWTGDNGSQW DFLQYSLGEI 600
    FNFNMYGIPM TGADICGFAQ NTTAELCARW MQVGAFYPFS RNHNSNDTIP 650
    QEPYAFPDST YVLDSSKKSL RLRYALLKQY YSHFVSSNGV GTVFRPTFFN 700
    FPDDASLLTN DQQFMIGDSL LGQPVLVQSA TPARFSHSSY LTFPSSGAFY 750
    DFVTDVATLN AQRYTNANNG QIKNVKFDDI MPLYIREGYT VFTQLASTAL 800
    RSRLLDSNFE LHVALAKSGT SYTAKGKFIT IQDYSDDNLI QKCIGANNCS 850
    FDIQVTGVVN GANLDLTIQI AGESAQTNFE TINVNKIIPY AADLKFAAST 900
    ATFTISKNGT INASIPLQAA QQE 923
    Length:923
    Mass (Da):104,117
    Last modified:July 1, 1997 - v1
    Checksum:i6294809F43EA54C3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D83384 mRNA. Translation: BAA20462.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D83384 mRNA. Translation: BAA20462.1 .

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH31. Glycoside Hydrolase Family 31.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR011013. Gal_mutarotase_SF_dom.
    IPR000322. Glyco_hydro_31.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF01055. Glyco_hydro_31. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 2 hits.
    SSF74650. SSF74650. 1 hit.
    PROSITEi PS00129. GLYCOSYL_HYDROL_F31_1. 1 hit.
    PS00707. GLYCOSYL_HYDROL_F31_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of a gene encoding acid alpha-glucosidase from Tetrahymena pyriformis."
      Alam S., Nakashima S., Deyashiki Y., Banno Y., Hara A., Nozawa Y.
      J. Eukaryot. Microbiol. 43:295-303(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
      Strain: W.

    Entry informationi

    Entry nameiAGLU_TETPY
    AccessioniPrimary (citable) accession number: O00906
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: July 1, 1997
    Last modified: October 1, 2014
    This is version 54 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3