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Protein

TFIIH basal transcription factor complex helicase repB subunit

Gene

repB

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Component of the core-TFIIH basal transcription factor involved in nucleotide excision repair (NER) of DNA by opening DNA around the damage, and in RNA transcription by RNA polymerase II by anchoring the CDK-activating kinase (CAK) complex to the core-TFIIH complex.By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi342 – 3498ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

  • cellular response to DNA damage stimulus Source: dictyBase
  • nucleotide-excision repair Source: dictyBase
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • transcription from RNA polymerase II promoter Source: dictyBase
  • transcription initiation from RNA polymerase II promoter Source: InterPro
  • UV protection Source: dictyBase
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

DNA damage, DNA repair, Transcription, Transcription regulation

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DDI-113418. Formation of the Early Elongation Complex.
R-DDI-5696395. Formation of Incision Complex in GG-NER.
R-DDI-674695. RNA Polymerase II Pre-transcription Events.
R-DDI-6781823. Formation of TC-NER Pre-Incision Complex.
R-DDI-6782135. Dual incision in TC-NER.
R-DDI-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.
R-DDI-6796648. TP53 Regulates Transcription of DNA Repair Genes.
R-DDI-72086. mRNA Capping.
R-DDI-73776. RNA Polymerase II Promoter Escape.
R-DDI-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-DDI-75953. RNA Polymerase II Transcription Initiation.
R-DDI-75955. RNA Polymerase II Transcription Elongation.
R-DDI-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.
R-DDI-77075. RNA Pol II CTD phosphorylation and interaction with CE.

Names & Taxonomyi

Protein namesi
Recommended name:
TFIIH basal transcription factor complex helicase repB subunit (EC:3.6.4.12)
Alternative name(s):
DNA excision repair cross-complementing protein-3 homolog
DNA repair helicase repB
DNA repair protein B
Gene namesi
Name:repB
Synonyms:ercc3
ORF Names:DDB_G0278729
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 3, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0278729. repB.

Subcellular locationi

GO - Cellular componenti

  • holo TFIIH complex Source: dictyBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 800800TFIIH basal transcription factor complex helicase repB subunitPRO_0000376006Add
BLAST

Proteomic databases

PaxDbiO00835.

Expressioni

Developmental stagei

Constitutively expressed at low levels throughout development.2 Publications

Inductioni

Up-regulated a dose dependent manner following exposure to both UV LIGHT and cisplatin. Unaffected by exposure to hydrogen peroxide.3 Publications

Interactioni

Subunit structurei

One of the six subunits forming the core-TFIIH basal transcription factor.By similarity

Protein-protein interaction databases

STRINGi44689.DDB0214830.

Structurei

3D structure databases

ProteinModelPortaliO00835.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini329 – 491163Helicase ATP-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini546 – 704159Helicase C-terminalPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi444 – 4474DEVH box

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi255 – 2595Poly-Glu

Sequence similaritiesi

Belongs to the helicase family. RAD25/XPB subfamily.Curated
Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1123. Eukaryota.
COG1061. LUCA.
InParanoidiO00835.
KOiK10843.
OMAiIRFHEQR.
PhylomeDBiO00835.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR032438. ERCC3_RAD25_C.
IPR006935. Helicase/UvrB_N.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR001161. XPB/Ssl2.
IPR032830. XPB/Ssl2_N.
[Graphical view]
PfamiPF16203. ERCC3_RAD25_C. 1 hit.
PF13625. Helicase_C_3. 1 hit.
PF04851. ResIII. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 3 hits.
TIGRFAMsiTIGR00603. rad25. 1 hit.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O00835-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSGDSNLKR RRGGNTGQSS KSYNTWTDYE EDLEESGEFN QSIKKTTNTS
60 70 80 90 100
SATLTSSEEK GSLLDYSKRC ILKQDNKSRP IWVCPDGHIF LETFSAIYKQ
110 120 130 140 150
ASDFLVAIAE PVCRPQNIHE YQLTPYSLYA AVSVGLETND IITVLGRLSK
160 170 180 190 200
LALPKEVEQF VRQCTQSYGK VKLVLQKNKY FVESAYPEVL EFLLKDSSIA
210 220 230 240 250
TARIKPTLEE SVVDPKTGFI INKEVVTGAQ ISGGLQANQS LDPVLKNDAL
260 270 280 290 300
SNLLEEEEED TVNNSDQHFH SFEIDPQQVE EVKKRCIQLD YPVLEEYDFR
310 320 330 340 350
NDTVNPNLNI DLKPTTMIRP YQEKSLSKMF GNGRARSGII VLPCGAGKSL
360 370 380 390 400
SGITAACTVK KSILVLCTSA VSVEQWKYQF KLWSNIEERQ ISKFTSDNKE
410 420 430 440 450
KISNVAGVTI TTYTMVAFGG RRSAESLKIM NEITNREWGL VLLDEVHVVP
460 470 480 490 500
AAMFRKVLTV TKAHCKLGLT ATLLREDEKI QDLNFLIGPK LYEANWLDLQ
510 520 530 540 550
KAGFLANVSC SEVWCPMTAE FYKEYLINDS QGKKKLLYTM NPNKFRACEY
560 570 580 590 600
LIRFHEQRGD KIIVFSDNVY ALQKYAKGLG RYFIYGPTSG HERMSILSKF
610 620 630 640 650
QHDPTVRTIF ISKVGDTSID IPEATVIIQV SSHYGSRRQE AQRLGRILRP
660 670 680 690 700
KPKSDGLYNA FFYSLVSKDT QEMYYSTKRQ QFLIDQGYSF KVISELPGID
710 720 730 740 750
QEVNLKYSSK QDQLDLLAQV LGEGEDSGKN EILEEDFDDI TRGAKKSKSS
760 770 780 790 800
APTVSRTTGG STRALSGGND MNYMEYQAPA IYKSIPTQHA LFKQRAKNKQ
Length:800
Mass (Da):90,257
Last modified:July 1, 1997 - v1
Checksum:i0DB636E33067B09A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U77065 Genomic DNA. Translation: AAB62732.1.
AAFI02000024 Genomic DNA. Translation: EAL67966.1.
RefSeqiXP_647819.1. XM_642727.1.

Genome annotation databases

EnsemblProtistsiDDB0214830; DDB0214830; DDB_G0278729.
GeneIDi8621779.
KEGGiddi:DDB_G0278729.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U77065 Genomic DNA. Translation: AAB62732.1.
AAFI02000024 Genomic DNA. Translation: EAL67966.1.
RefSeqiXP_647819.1. XM_642727.1.

3D structure databases

ProteinModelPortaliO00835.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi44689.DDB0214830.

Proteomic databases

PaxDbiO00835.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiDDB0214830; DDB0214830; DDB_G0278729.
GeneIDi8621779.
KEGGiddi:DDB_G0278729.

Organism-specific databases

dictyBaseiDDB_G0278729. repB.

Phylogenomic databases

eggNOGiKOG1123. Eukaryota.
COG1061. LUCA.
InParanoidiO00835.
KOiK10843.
OMAiIRFHEQR.
PhylomeDBiO00835.

Enzyme and pathway databases

ReactomeiR-DDI-113418. Formation of the Early Elongation Complex.
R-DDI-5696395. Formation of Incision Complex in GG-NER.
R-DDI-674695. RNA Polymerase II Pre-transcription Events.
R-DDI-6781823. Formation of TC-NER Pre-Incision Complex.
R-DDI-6782135. Dual incision in TC-NER.
R-DDI-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.
R-DDI-6796648. TP53 Regulates Transcription of DNA Repair Genes.
R-DDI-72086. mRNA Capping.
R-DDI-73776. RNA Polymerase II Promoter Escape.
R-DDI-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-DDI-75953. RNA Polymerase II Transcription Initiation.
R-DDI-75955. RNA Polymerase II Transcription Elongation.
R-DDI-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.
R-DDI-77075. RNA Pol II CTD phosphorylation and interaction with CE.

Miscellaneous databases

PROiO00835.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR032438. ERCC3_RAD25_C.
IPR006935. Helicase/UvrB_N.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR001161. XPB/Ssl2.
IPR032830. XPB/Ssl2_N.
[Graphical view]
PfamiPF16203. ERCC3_RAD25_C. 1 hit.
PF13625. Helicase_C_3. 1 hit.
PF04851. ResIII. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 3 hits.
TIGRFAMsiTIGR00603. rad25. 1 hit.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiERCC3_DICDI
AccessioniPrimary (citable) accession number: O00835
Secondary accession number(s): Q54XI4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 26, 2009
Last sequence update: July 1, 1997
Last modified: June 8, 2016
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.