O00835 (ERCC3_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
TFIIH basal transcription factor complex helicase repB subunit
EC=3.6.4.12

Alternative name(s):
DNA excision repair cross-complementing protein-3 homolog
DNA repair helicase repB
DNA repair protein B

Gene names

Name:	repB
Synonyms:	ercc3
ORF Names:	DDB_G0278729

Organism

Dictyostelium discoideum (Slime mold)

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	800 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Component of the core-TFIIH basal transcription factor involved in nucleotide excision repair (NER) of DNA by opening DNA around the damage, and in RNA transcription by RNA polymerase II by anchoring the CDK-activating kinase (CAK) complex to the core-TFIIH complex By similarity.
Catalytic activity	ATP + H2O = ADP + phosphate.
Subunit structure	One of the six subunits forming the core-TFIIH basal transcription factor By similarity.
Subcellular location	Nucleus By similarity.
Developmental stage	Constitutively expressed at low levels throughout development. Ref.1 Ref.5
Induction	Up-regulated a dose dependent manner following exposure to both UV-light and cisplatin. Unaffected by exposure to hydrogen peroxide. Ref.3 Ref.4 Ref.5
Sequence similarities	Belongs to the helicase family. RAD25/XPB subfamily. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.

Ontologies

Keywords
Biological process	DNA damage DNA repair Transcription Transcription regulation
Cellular component	Nucleus
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Helicase Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	UV protection Inferred from mutant phenotype Ref.3. Source: dictyBase nucleotide-excision repair Inferred from sequence or structural similarity Ref.1. Source: dictyBase regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription from RNA polymerase II promoter Inferred from sequence or structural similarity Ref.1. Source: dictyBase
Cellular component	SSL2-core TFIIH complex portion of NEF3 complex Inferred from sequence or structural similarity. Source: dictyBase SSL2-core TFIIH complex portion of holo TFIIH complex Inferred from sequence or structural similarity. Source: dictyBase
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent 3'-5' DNA helicase activity Inferred from sequence or structural similarity Ref.1. Source: dictyBase damaged DNA binding Inferred from sequence or structural similarity Ref.1. Source: dictyBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 800	800	TFIIH basal transcription factor complex helicase repB subunit		PRO_0000376006
Regions
	Domain	329 – 491	163	Helicase ATP-binding
	Domain	546 – 704	159	Helicase C-terminal
	Nucleotide binding	342 – 349	8	ATP By similarity
	Motif	444 – 447	4	DEVH box
	Compositional bias	255 – 259	5	Poly-Glu

Sequences

	Sequence		Length	Mass (Da)
	O00835 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: 0DB636E33067B09A	FASTA	800	90,257
	10 20 30 40 50 60 MSSGDSNLKR RRGGNTGQSS KSYNTWTDYE EDLEESGEFN QSIKKTTNTS SATLTSSEEK 70 80 90 100 110 120 GSLLDYSKRC ILKQDNKSRP IWVCPDGHIF LETFSAIYKQ ASDFLVAIAE PVCRPQNIHE 130 140 150 160 170 180 YQLTPYSLYA AVSVGLETND IITVLGRLSK LALPKEVEQF VRQCTQSYGK VKLVLQKNKY 190 200 210 220 230 240 FVESAYPEVL EFLLKDSSIA TARIKPTLEE SVVDPKTGFI INKEVVTGAQ ISGGLQANQS 250 260 270 280 290 300 LDPVLKNDAL SNLLEEEEED TVNNSDQHFH SFEIDPQQVE EVKKRCIQLD YPVLEEYDFR 310 320 330 340 350 360 NDTVNPNLNI DLKPTTMIRP YQEKSLSKMF GNGRARSGII VLPCGAGKSL SGITAACTVK 370 380 390 400 410 420 KSILVLCTSA VSVEQWKYQF KLWSNIEERQ ISKFTSDNKE KISNVAGVTI TTYTMVAFGG 430 440 450 460 470 480 RRSAESLKIM NEITNREWGL VLLDEVHVVP AAMFRKVLTV TKAHCKLGLT ATLLREDEKI 490 500 510 520 530 540 QDLNFLIGPK LYEANWLDLQ KAGFLANVSC SEVWCPMTAE FYKEYLINDS QGKKKLLYTM 550 560 570 580 590 600 NPNKFRACEY LIRFHEQRGD KIIVFSDNVY ALQKYAKGLG RYFIYGPTSG HERMSILSKF 610 620 630 640 650 660 QHDPTVRTIF ISKVGDTSID IPEATVIIQV SSHYGSRRQE AQRLGRILRP KPKSDGLYNA 670 680 690 700 710 720 FFYSLVSKDT QEMYYSTKRQ QFLIDQGYSF KVISELPGID QEVNLKYSSK QDQLDLLAQV 730 740 750 760 770 780 LGEGEDSGKN EILEEDFDDI TRGAKKSKSS APTVSRTTGG STRALSGGND MNYMEYQAPA 790 800 IYKSIPTQHA LFKQRAKNKQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Differential developmental expression of the rep B and rep D xeroderma pigmentosum related DNA helicase genes from Dictyostelium discoideum." Lee S.-K., Yu S.-L., Garcia M.X.U., Alexander H., Alexander S. Nucleic Acids Res. 25:2365-2374(1997) [PubMed: 9171087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], DEVELOPMENTAL STAGE. Strain: AX4.
[2]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[3]	"A mutation in repB, the dictyostelium homolog of the human xeroderma pigmentosum B gene, has increased sensitivity to UV-light but normal morphogenesis." Lee S.-K., Yu S.-L., Alexander H., Alexander S. Biochim. Biophys. Acta 1399:161-172(1998) [PubMed: 9765592] [Abstract] Cited for: MUTAGENESIS OF 755-SER--GLN-800 AND 706-LYS--GLN-800, INDUCTION BY UV.
[4]	"Rapid changes of nucleotide excision repair gene expression following UV-irradiation and cisplatin treatment of Dictyostelium discoideum." Yu S.-L., Lee S.-K., Alexander H., Alexander S. Nucleic Acids Res. 26:3397-3403(1998) [PubMed: 9649625] [Abstract] Cited for: INDUCTION.
[5]	"Differential developmental expression and cell type specificity of Dictyostelium catalases and their response to oxidative stress and UV-light." Garcia M.X.U., Foote C., van Es S., Devreotes P.N., Alexander S., Alexander H. Biochim. Biophys. Acta 1492:295-310(2000) [PubMed: 11004503] [Abstract] Cited for: INDUCTION, DEVELOPMENTAL STAGE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U77065 Genomic DNA. Translation: AAB62732.1. AAFI02000024 Genomic DNA. Translation: EAL67966.1.
RefSeq	XP_647819.1. XM_642727.1.
3D structure databases
HSSP	HSSP built from PDB template 2FWR based on UniProtKB O29889.
ProteinModelPortal	O00835.
ModBase	Search...
Protein-protein interaction databases
STRING	O00835.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0214830; DDB0214830; DDB_G0278729.
GeneID	8621779.
GenomeReviews	Gene locus repB in contig CM000152_GR.
KEGG	ddi:DDB_G0278729.
Organism-specific databases
dictyBase	DDB_G0278729. repB.
Phylogenomic databases
eggNOG	KOG1123.
GeneTree	EPrGT00050000001860.
HOGENOM	HBG314410.
InParanoid	O00835.
OMA	KLCTVSY.
PhylomeDB	O00835.
ProtClustDB	CLSZ2729383.
Family and domain databases
InterPro	IPR014001. DEAD-like_helicase. IPR006935. Helicase/UvrB_dom. IPR001650. Helicase_C. IPR001161. XPGB_DNA_repair. [Graphical view]
KO	K10843.
Pfam	PF00271. Helicase_C. 1 hit. PF04851. ResIII. 1 hit. [Graphical view]
PRINTS	PR00851. XRODRMPGMNTB.
SMART	SM00487. DEXDc. 1 hit. SM00490. HELICc. 1 hit. [Graphical view]
TIGRFAMs	TIGR00603. Rad25. 1 hit.
PROSITE	PS51192. HELICASE_ATP_BIND_1. 1 hit. PS51194. HELICASE_CTER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ERCC3_DICDI

Accession

Primary (citable) accession number: O00835
Secondary accession number(s): Q54XI4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 26, 2009
Last sequence update:	July 1, 1997
Last modified:	December 14, 2011
This is version 93 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)