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Protein

Ubiquitin-conjugating enzyme E2 C

Gene

UBE2C

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by initiating 'Lys-11'-linked polyubiquitin chains on APC/C substrates, leading to the degradation of APC/C substrates by the proteasome and promoting mitotic exit.6 Publications

Catalytic activityi

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotation2 Publications
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E1 ubiquitin-activating enzyme]-L-cysteine + N6-monoubiquitinyl-[acceptor protein]-L-lysine.1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation1 Publication
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei114Glycyl thioester intermediate1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ubiquitin conjugating enzyme activity Source: MGI
  • ubiquitin-like protein ligase binding Source: UniProtKB
  • ubiquitin protein ligase activity Source: GO_Central
  • ubiquitin protein ligase binding Source: GO_Central
  • ubiquitin-protein transferase activity Source: UniProtKB

GO - Biological processi

  • anaphase-promoting complex-dependent catabolic process Source: UniProtKB
  • cell division Source: UniProtKB-KW
  • exit from mitosis Source: UniProtKB
  • free ubiquitin chain polymerization Source: UniProtKB
  • positive regulation of exit from mitosis Source: UniProtKB
  • positive regulation of ubiquitin protein ligase activity Source: UniProtKB
  • protein K11-linked ubiquitination Source: UniProtKB
  • protein K48-linked ubiquitination Source: UniProtKB
  • protein ubiquitination Source: UniProtKB
  • regulation of mitotic metaphase/anaphase transition Source: GO_Central
  • ubiquitin-dependent protein catabolic process Source: UniProtKB

Keywordsi

Molecular functionTransferase
Biological processCell cycle, Cell division, Mitosis, Ubl conjugation pathway
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.3.2.B6 2681
ReactomeiR-HSA-141430 Inactivation of APC/C via direct inhibition of the APC/C complex
R-HSA-174048 APC/C:Cdc20 mediated degradation of Cyclin B
R-HSA-174084 Autodegradation of Cdh1 by Cdh1:APC/C
R-HSA-174154 APC/C:Cdc20 mediated degradation of Securin
R-HSA-174178 APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1
R-HSA-174184 Cdc20:Phospho-APC/C mediated degradation of Cyclin A
R-HSA-176407 Conversion from APC/C:Cdc20 to APC/C:Cdh1 in late anaphase
R-HSA-176408 Regulation of APC/C activators between G1/S and early anaphase
R-HSA-176409 APC/C:Cdc20 mediated degradation of mitotic proteins
R-HSA-176412 Phosphorylation of the APC/C
R-HSA-179409 APC-Cdc20 mediated degradation of Nek2A
R-HSA-2467813 Separation of Sister Chromatids
R-HSA-2559582 Senescence-Associated Secretory Phenotype (SASP)
R-HSA-69017 CDK-mediated phosphorylation and removal of Cdc6
R-HSA-8866652 Synthesis of active ubiquitin: roles of E1 and E2 enzymes
R-HSA-983168 Antigen processing: Ubiquitination & Proteasome degradation
SignaLinkiO00762
SIGNORiO00762
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 C (EC:2.3.2.232 Publications)
Alternative name(s):
(E3-independent) E2 ubiquitin-conjugating enzyme C (EC:2.3.2.241 Publication)
E2 ubiquitin-conjugating enzyme C
UbcH10
Ubiquitin carrier protein C
Ubiquitin-protein ligase C
Gene namesi
Name:UBE2C
Synonyms:UBCH10
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 20

Organism-specific databases

EuPathDBiHostDB:ENSG00000175063.16
HGNCiHGNC:15937 UBE2C
MIMi605574 gene
neXtProtiNX_O00762

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi114C → S: Loss of function; inhibition of cyclin-B degradation. 2 Publications1

Organism-specific databases

DisGeNETi11065
OpenTargetsiENSG00000175063
PharmGKBiPA38057

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00000825602 – 179Ubiquitin-conjugating enzyme E2 CAdd BLAST178

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Modified residuei3PhosphoserineCombined sources1

Post-translational modificationi

Autoubiquitinated by the APC/C complex, leading to its degradation by the proteasome. Its degradation plays a central role in APC/C regulation, allowing cyclin-A accumulation before S phase entry. APC/C substrates inhibit the autoubiquitination of UBE2C/UBCH10 but not its E2 function, hence APC/C remaining active until its substrates have been destroyed.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiO00762
PaxDbiO00762
PeptideAtlasiO00762
PRIDEiO00762
TopDownProteomicsiO00762-1 [O00762-1]
O00762-3 [O00762-3]

PTM databases

iPTMnetiO00762
PhosphoSitePlusiO00762

Expressioni

Gene expression databases

BgeeiENSG00000175063
CleanExiHS_UBE2C
ExpressionAtlasiO00762 baseline and differential
GenevisibleiO00762 HS

Organism-specific databases

HPAiCAB011464
CAB035990
HPA034569
HPA054975

Interactioni

Subunit structurei

Component of the APC/C complex, composed of at least 14 distinct subunits that assemble into a complex of at least 19 chains with a combined molecular mass of around 1.2 MDa. Within this complex, directly interacts with ANAPC2.2 Publications

GO - Molecular functioni

  • ubiquitin-like protein ligase binding Source: UniProtKB
  • ubiquitin protein ligase binding Source: GO_Central

Protein-protein interaction databases

BioGridi116249, 68 interactors
DIPiDIP-52725N
IntActiO00762, 10 interactors
STRINGi9606.ENSP00000348838

Structurei

Secondary structure

1179
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi30 – 45Combined sources16
Beta strandi50 – 54Combined sources5
Beta strandi61 – 68Combined sources8
Beta strandi78 – 84Combined sources7
Turni87 – 91Combined sources5
Beta strandi95 – 100Combined sources6
Beta strandi111 – 113Combined sources3
Helixi116 – 118Combined sources3
Turni119 – 121Combined sources3
Helixi128 – 140Combined sources13
Helixi150 – 155Combined sources6
Helixi159 – 172Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1I7KX-ray1.95A/B1-179[»]
4YIIX-ray1.80U27-179[»]
5A31electron microscopy4.30Q29-173[»]
5KHRelectron microscopy6.10Q1-179[»]
5L9Uelectron microscopy6.40U1-179[»]
ProteinModelPortaliO00762
SMRiO00762
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO00762

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0421 Eukaryota
ENOG4111IGV LUCA
GeneTreeiENSGT00760000119350
HOGENOMiHOG000233454
HOVERGENiHBG063308
InParanoidiO00762
KOiK06688
OMAiAELWDKD
OrthoDBiEOG091G0M7S
PhylomeDBiO00762
TreeFamiTF101116

Family and domain databases

CDDicd00195 UBCc, 1 hit
Gene3Di3.10.110.10, 1 hit
InterProiView protein in InterPro
IPR000608 UBQ-conjugat_E2
IPR023313 UBQ-conjugating_AS
IPR016135 UBQ-conjugating_enzyme/RWD
PfamiView protein in Pfam
PF00179 UQ_con, 1 hit
SUPFAMiSSF54495 SSF54495, 1 hit
PROSITEiView protein in PROSITE
PS00183 UBIQUITIN_CONJUGAT_1, 1 hit
PS50127 UBIQUITIN_CONJUGAT_2, 1 hit

Sequences (4)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O00762-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASQNRDPAA TSVAAARKGA EPSGGAARGP VGKRLQQELM TLMMSGDKGI
60 70 80 90 100
SAFPESDNLF KWVGTIHGAA GTVYEDLRYK LSLEFPSGYP YNAPTVKFLT
110 120 130 140 150
PCYHPNVDTQ GNICLDILKE KWSALYDVRT ILLSIQSLLG EPNIDSPLNT
160 170
HAAELWKNPT AFKKYLQETY SKQVTSQEP
Length:179
Mass (Da):19,652
Last modified:July 1, 1997 - v1
Checksum:i0B6F58A1F0665D9A
GO
Isoform 2 (identifier: O00762-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-39: Missing.

Note: No experimental confirmation available.
Show »
Length:140
Mass (Da):15,765
Checksum:i194F276AD75D5168
GO
Isoform 3 (identifier: O00762-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     44-72: Missing.

Note: No experimental confirmation available.
Show »
Length:150
Mass (Da):16,676
Checksum:i5410B7BA11DFC35E
GO
Isoform 4 (identifier: O00762-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     73-140: VYEDLRYKLS...ILLSIQSLLG → AVGSIRTSST...LTLQLFLQMP

Note: No experimental confirmation available.
Show »
Length:161
Mass (Da):17,373
Checksum:iD8F52001401888DA
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_00769425G → D. 1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0456471 – 39Missing in isoform 2. 1 PublicationAdd BLAST39
Alternative sequenceiVSP_04564844 – 72Missing in isoform 3. 1 PublicationAdd BLAST29
Alternative sequenceiVSP_04564973 – 140VYEDL…QSLLG → AVGSIRTSSTVCLLSGPRET QDSSKPLVWGLGWDMRLLLE LTLQLFLQMP in isoform 4. 1 PublicationAdd BLAST68

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U73379 mRNA Translation: AAB53362.1
BT007300 mRNA Translation: AAP35964.1
AL050348 Genomic DNA No translation available.
CH471077 Genomic DNA Translation: EAW75804.1
CH471077 Genomic DNA Translation: EAW75805.1
CH471077 Genomic DNA Translation: EAW75806.1
CH471077 Genomic DNA Translation: EAW75807.1
BC007656 mRNA Translation: AAH07656.1
BC016292 mRNA Translation: AAH16292.1
BC050736 mRNA Translation: AAH50736.1
BI858659 mRNA No translation available.
BM556795 mRNA No translation available.
BU844974 mRNA No translation available.
CCDSiCCDS13370.1 [O00762-1]
CCDS13371.1 [O00762-4]
CCDS13372.1 [O00762-3]
CCDS13374.1 [O00762-2]
RefSeqiNP_001268670.1, NM_001281741.1
NP_001268671.1, NM_001281742.1
NP_008950.1, NM_007019.3 [O00762-1]
NP_861515.1, NM_181799.2 [O00762-4]
NP_861516.1, NM_181800.2 [O00762-3]
NP_861517.1, NM_181801.3 [O00762-2]
UniGeneiHs.93002

Genome annotation databases

EnsembliENST00000335046; ENSP00000335674; ENSG00000175063 [O00762-4]
ENST00000352551; ENSP00000333975; ENSG00000175063 [O00762-3]
ENST00000356455; ENSP00000348838; ENSG00000175063 [O00762-1]
ENST00000372568; ENSP00000361649; ENSG00000175063 [O00762-2]
GeneIDi11065
KEGGihsa:11065
UCSCiuc002xpl.5 human [O00762-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiUBE2C_HUMAN
AccessioniPrimary (citable) accession number: O00762
Secondary accession number(s): A6NP33, E1P5N7, G3XAB7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: July 1, 1997
Last modified: May 23, 2018
This is version 182 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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