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Protein

Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit beta

Gene

PIK3C2B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Phosphorylates PtdIns and PtdIns4P with a preference for PtdIns. Does not phosphorylate PtdIns(4,5)P2. May be involved in EGF and PDGF signaling cascades.1 Publication

Catalytic activityi

ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate = ADP + 1-phosphatidyl-1D-myo-inositol 3,4-bisphosphate.

Cofactori

Ca2+1 Publication, Mg2+1 Publication, Mn2+1 Publication

Enzyme regulationi

Activated by GRB2.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:HS05725-MONOMER.
ZFISH:HS05725-MONOMER.
BRENDAi2.7.1.137. 2681.
2.7.1.154. 2681.
ReactomeiR-HSA-1660499. Synthesis of PIPs at the plasma membrane.
SignaLinkiO00750.
SIGNORiO00750.

Chemistry databases

SwissLipidsiSLP:000000893.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit beta (EC:2.7.1.154)
Short name:
PI3K-C2-beta
Short name:
PtdIns-3-kinase C2 subunit beta
Alternative name(s):
C2-PI3K
Phosphoinositide 3-kinase-C2-beta
Gene namesi
Name:PIK3C2B
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:8972. PIK3C2B.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Endoplasmic reticulum, Membrane, Microsome, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi5287.
OpenTargetsiENSG00000133056.
PharmGKBiPA33305.

Chemistry databases

ChEMBLiCHEMBL5554.
GuidetoPHARMACOLOGYi2151.

Polymorphism and mutation databases

BioMutaiPIK3C2B.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000887981 – 1634Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit betaAdd BLAST1634

Proteomic databases

MaxQBiO00750.
PaxDbiO00750.
PeptideAtlasiO00750.
PRIDEiO00750.

PTM databases

iPTMnetiO00750.
PhosphoSitePlusiO00750.

Expressioni

Tissue specificityi

Expressed in columnar and transitional epithelia, mononuclear cells, and ganglion cells (at protein level). Widely expressed, with highest levels in thymus and placenta and lowest in peripheral blood, skeletal muscle and kidney.2 Publications

Gene expression databases

BgeeiENSG00000133056.
CleanExiHS_PIK3C2B.
ExpressionAtlasiO00750. baseline and differential.
GenevisibleiO00750. HS.

Organism-specific databases

HPAiHPA030515.

Interactioni

Subunit structurei

Part of a complex with ERBB2 and EGFR. Part of a complex with phosphorylated EGFR and GRB2. Interacts with phosphorylated EGFR and PDGFR, maybe indirectly. Interacts with GRB2 and SHC1.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
EGFRP005339EBI-641107,EBI-297353
ERBB2P046262EBI-641107,EBI-641062
GRB2P629932EBI-641107,EBI-401755
NCK1P163333EBI-641107,EBI-389883
TRIM27P143735EBI-641107,EBI-719493

Protein-protein interaction databases

BioGridi111305. 16 interactors.
IntActiO00750. 11 interactors.
MINTiMINT-2794980.
STRINGi9606.ENSP00000356155.

Chemistry databases

BindingDBiO00750.

Structurei

3D structure databases

ProteinModelPortaliO00750.
SMRiO00750.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini375 – 463PI3K-RBDPROSITE-ProRule annotationAdd BLAST89
Domaini635 – 786C2 PI3K-typePROSITE-ProRule annotationAdd BLAST152
Domaini805 – 981PIK helicalPROSITE-ProRule annotationAdd BLAST177
Domaini1079 – 1343PI3K/PI4KPROSITE-ProRule annotationAdd BLAST265
Domaini1365 – 1481PXPROSITE-ProRule annotationAdd BLAST117
Domaini1517 – 1608C2PROSITE-ProRule annotationAdd BLAST92

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 298Interaction with GRB21 PublicationAdd BLAST297

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi156 – 162Pro-rich7
Compositional biasi169 – 174Pro-rich6

Sequence similaritiesi

Belongs to the PI3/PI4-kinase family.PROSITE-ProRule annotation
Contains 1 C2 domain.PROSITE-ProRule annotation
Contains 1 C2 PI3K-type domain.PROSITE-ProRule annotation
Contains 1 PI3K-RBD domain.PROSITE-ProRule annotation
Contains 1 PI3K/PI4K domain.PROSITE-ProRule annotation
Contains 1 PIK helical domain.PROSITE-ProRule annotation
Contains 1 PX (phox homology) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0905. Eukaryota.
COG5032. LUCA.
GeneTreeiENSGT00760000119110.
HOVERGENiHBG082099.
InParanoidiO00750.
KOiK00923.
OMAiIHLQERP.
OrthoDBiEOG091G00FC.
PhylomeDBiO00750.
TreeFamiTF102031.

Family and domain databases

Gene3Di1.10.1070.11. 1 hit.
1.25.40.70. 1 hit.
2.60.40.150. 2 hits.
3.30.1520.10. 1 hit.
InterProiIPR016024. ARM-type_fold.
IPR000008. C2_dom.
IPR011009. Kinase-like_dom.
IPR001683. Phox.
IPR000403. PI3/4_kinase_cat_dom.
IPR018936. PI3/4_kinase_CS.
IPR002420. PI3K_C2_dom.
IPR000341. PI3K_Ras-bd_dom.
IPR015433. PI_Kinase.
IPR001263. PInositide-3_kin_accessory_dom.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PANTHERiPTHR10048. PTHR10048. 2 hits.
PfamiPF00168. C2. 1 hit.
PF00454. PI3_PI4_kinase. 1 hit.
PF00792. PI3K_C2. 1 hit.
PF00794. PI3K_rbd. 1 hit.
PF00613. PI3Ka. 1 hit.
PF00787. PX. 1 hit.
[Graphical view]
SMARTiSM00239. C2. 1 hit.
SM00142. PI3K_C2. 1 hit.
SM00144. PI3K_rbd. 1 hit.
SM00145. PI3Ka. 1 hit.
SM00146. PI3Kc. 1 hit.
SM00312. PX. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
SSF49562. SSF49562. 2 hits.
SSF54236. SSF54236. 1 hit.
SSF56112. SSF56112. 1 hit.
SSF64268. SSF64268. 1 hit.
PROSITEiPS50004. C2. 1 hit.
PS00915. PI3_4_KINASE_1. 1 hit.
PS00916. PI3_4_KINASE_2. 1 hit.
PS50290. PI3_4_KINASE_3. 1 hit.
PS51547. PI3K_C2. 1 hit.
PS51546. PI3K_RBD. 1 hit.
PS51545. PIK_HELICAL. 1 hit.
PS50195. PX. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O00750-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSTQGNGEH WKSLESVGIS RKELAMAEAL QMEYDALSRL RHDKEENRAK
60 70 80 90 100
QNADPSLISW DEPGVDFYSK PAGRRTDLKL LRGLSGSDPT LNYNSLSPQE
110 120 130 140 150
GPPNHSTSQG PQPGSDPWPK GSLSGDYLYI FDGSDGGVSS SPGPGDIEGS
160 170 180 190 200
CKKLSPPPLP PRASIWDTPP LPPRKGSPSS SKISQPSDIN TFSLVEQLPG
210 220 230 240 250
KLLEHRILEE EEVLGGGGQG RLLGSVDYDG INDAITRLNL KSTYDAEMLR
260 270 280 290 300
DATRGWKEGR GPLDFSKDTS GKPVARSKTM PPQVPPRTYA SRYGNRKNAT
310 320 330 340 350
PGKNRRISAA PVGSRPHTVA NGHELFEVSE ERDEEVAAFC HMLDILRSGS
360 370 380 390 400
DIQDYFLTGY VWSAVTPSPE HLGDEVNLKV TVLCDRLQEA LTFTCNCSST
410 420 430 440 450
VDLLIYQTLC YTHDDLRNVD VGDFVLKPCG LEEFLQNKHA LGSHEYIQYC
460 470 480 490 500
RKFDIDIRLQ LMEQKVVRSD LARTVNDDQS PSTLNYLVHL QERPVKQTIS
510 520 530 540 550
RQALSLLFDT YHNEVDAFLL ADGDFPLKAD RVVQSVKAIC NALAAVETPE
560 570 580 590 600
ITSALNQLPP CPSRMQPKIQ KDPSVLAVRE NREKVVEALT AAILDLVELY
610 620 630 640 650
CNTFNADFQT AVPGSRKHDL VQEACHFARS LAFTVYATHR IPIIWATSYE
660 670 680 690 700
DFYLSCSLSH GGKELCSPLQ TRRAHFSKYL FHLIVWDQQI CFPVQVNRLP
710 720 730 740 750
RETLLCATLY ALPIPPPGSS SEANKQRRVP EALGWVTTPL FNFRQVLTCG
760 770 780 790 800
RKLLGLWPAT QENPSARWSA PNFHQPDSVI LQIDFPTSAF DIKFTSPPGD
810 820 830 840 850
KFSPRYEFGS LREEDQRKLK DIMQKESLYW LTDADKKRLW EKRYYCHSEV
860 870 880 890 900
SSLPLVLASA PSWEWACLPD IYVLLKQWTH MNHQDALGLL HATFPDQEVR
910 920 930 940 950
RMAVQWIGSL SDAELLDYLP QLVQALKYEC YLDSPLVRFL LKRAVSDLRV
960 970 980 990 1000
THYFFWLLKD GLKDSQFSIR YQYLLAALLC CCGKGLREEF NRQCWLVNAL
1010 1020 1030 1040 1050
AKLAQQVREA APSARQGILR TGLEEVKQFF ALNGSCRLPL SPSLLVKGIV
1060 1070 1080 1090 1100
PRDCSYFNSN AVPLKLSFQN VDPLGENIRV IFKCGDDLRQ DMLTLQMIRI
1110 1120 1130 1140 1150
MSKIWVQEGL DMRMVIFRCF STGRGRGMVE MIPNAETLRK IQVEHGVTGS
1160 1170 1180 1190 1200
FKDRPLADWL QKHNPGEDEY EKAVENFIYS CAGCCVATYV LGICDRHNDN
1210 1220 1230 1240 1250
IMLKTTGHMF HIDFGRFLGH AQMFGNIKRD RAPFVFTSDM AYVINGGDKP
1260 1270 1280 1290 1300
SSRFHDFVDL CCQAYNLIRK HTHLFLNLLG LMLSCGIPEL SDLEDLKYVY
1310 1320 1330 1340 1350
DALRPQDTEA NATTYFTRLI ESSLGSVATK LNFFIHNLAQ MKFTGSDDRL
1360 1370 1380 1390 1400
TLSFASRTHT LKSSGRISDV FLCRHEKIFH PNKGYIYVVK VMRENTHEAT
1410 1420 1430 1440 1450
YIQRTFEEFQ ELHNKLRLLF PSSHLPSFPS RFVIGRSRGE AVAERRREEL
1460 1470 1480 1490 1500
NGYIWHLIHA PPEVAECDLV YTFFHPLPRD EKAMGTSPAP KSSDGTWARP
1510 1520 1530 1540 1550
VGKVGGEVKL SISYKNNKLF IMVMHIRGLQ LLQDGNDPDP YVKIYLLPDP
1560 1570 1580 1590 1600
QKTTKRKTKV ARKTCNPTYN EMLVYDGIPK GDLQQRELQL SVLSEQGFWE
1610 1620 1630
NVLLGEVNIR LRELDLAQEK TGWFALGSRS HGTL
Length:1,634
Mass (Da):184,768
Last modified:August 30, 2005 - v2
Checksum:iCA817BA77C12E833
GO

Sequence cautioni

The sequence CAA74194 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti6G → D in CAA72168 (PubMed:9144573).Curated1
Sequence conflicti63P → S in CAA74194 (PubMed:9830063).Curated1
Sequence conflicti75R → W in CAA74194 (PubMed:9830063).Curated1
Sequence conflicti99Q → L in CAA74194 (PubMed:9830063).Curated1
Sequence conflicti246A → V in CAA72168 (PubMed:9144573).Curated1
Sequence conflicti278K → E in CAA74194 (PubMed:9830063).Curated1
Sequence conflicti567P → S in CAA74194 (PubMed:9830063).Curated1
Sequence conflicti664 – 665EL → DM in CAA72168 (PubMed:9144573).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y11312 mRNA. Translation: CAA72168.1.
Y13892 mRNA. Translation: CAA74194.1. Different initiation.
AL606489 Genomic DNA. Translation: CAI16572.1.
CCDSiCCDS1446.1.
PIRiJC5500.
PC4346.
RefSeqiNP_002637.3. NM_002646.3.
XP_005245314.1. XM_005245257.2.
XP_011507932.1. XM_011509630.2.
XP_011507933.1. XM_011509631.2.
XP_016856962.1. XM_017001473.1.
XP_016856963.1. XM_017001474.1.
UniGeneiHs.497487.

Genome annotation databases

EnsembliENST00000367187; ENSP00000356155; ENSG00000133056.
GeneIDi5287.
KEGGihsa:5287.
UCSCiuc001haw.4. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y11312 mRNA. Translation: CAA72168.1.
Y13892 mRNA. Translation: CAA74194.1. Different initiation.
AL606489 Genomic DNA. Translation: CAI16572.1.
CCDSiCCDS1446.1.
PIRiJC5500.
PC4346.
RefSeqiNP_002637.3. NM_002646.3.
XP_005245314.1. XM_005245257.2.
XP_011507932.1. XM_011509630.2.
XP_011507933.1. XM_011509631.2.
XP_016856962.1. XM_017001473.1.
XP_016856963.1. XM_017001474.1.
UniGeneiHs.497487.

3D structure databases

ProteinModelPortaliO00750.
SMRiO00750.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111305. 16 interactors.
IntActiO00750. 11 interactors.
MINTiMINT-2794980.
STRINGi9606.ENSP00000356155.

Chemistry databases

BindingDBiO00750.
ChEMBLiCHEMBL5554.
GuidetoPHARMACOLOGYi2151.
SwissLipidsiSLP:000000893.

PTM databases

iPTMnetiO00750.
PhosphoSitePlusiO00750.

Polymorphism and mutation databases

BioMutaiPIK3C2B.

Proteomic databases

MaxQBiO00750.
PaxDbiO00750.
PeptideAtlasiO00750.
PRIDEiO00750.

Protocols and materials databases

DNASUi5287.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000367187; ENSP00000356155; ENSG00000133056.
GeneIDi5287.
KEGGihsa:5287.
UCSCiuc001haw.4. human.

Organism-specific databases

CTDi5287.
DisGeNETi5287.
GeneCardsiPIK3C2B.
HGNCiHGNC:8972. PIK3C2B.
HPAiHPA030515.
MIMi602838. gene.
neXtProtiNX_O00750.
OpenTargetsiENSG00000133056.
PharmGKBiPA33305.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0905. Eukaryota.
COG5032. LUCA.
GeneTreeiENSGT00760000119110.
HOVERGENiHBG082099.
InParanoidiO00750.
KOiK00923.
OMAiIHLQERP.
OrthoDBiEOG091G00FC.
PhylomeDBiO00750.
TreeFamiTF102031.

Enzyme and pathway databases

BioCyciMetaCyc:HS05725-MONOMER.
ZFISH:HS05725-MONOMER.
BRENDAi2.7.1.137. 2681.
2.7.1.154. 2681.
ReactomeiR-HSA-1660499. Synthesis of PIPs at the plasma membrane.
SignaLinkiO00750.
SIGNORiO00750.

Miscellaneous databases

ChiTaRSiPIK3C2B. human.
GeneWikiiPIK3C2B.
GenomeRNAii5287.
PROiO00750.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000133056.
CleanExiHS_PIK3C2B.
ExpressionAtlasiO00750. baseline and differential.
GenevisibleiO00750. HS.

Family and domain databases

Gene3Di1.10.1070.11. 1 hit.
1.25.40.70. 1 hit.
2.60.40.150. 2 hits.
3.30.1520.10. 1 hit.
InterProiIPR016024. ARM-type_fold.
IPR000008. C2_dom.
IPR011009. Kinase-like_dom.
IPR001683. Phox.
IPR000403. PI3/4_kinase_cat_dom.
IPR018936. PI3/4_kinase_CS.
IPR002420. PI3K_C2_dom.
IPR000341. PI3K_Ras-bd_dom.
IPR015433. PI_Kinase.
IPR001263. PInositide-3_kin_accessory_dom.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PANTHERiPTHR10048. PTHR10048. 2 hits.
PfamiPF00168. C2. 1 hit.
PF00454. PI3_PI4_kinase. 1 hit.
PF00792. PI3K_C2. 1 hit.
PF00794. PI3K_rbd. 1 hit.
PF00613. PI3Ka. 1 hit.
PF00787. PX. 1 hit.
[Graphical view]
SMARTiSM00239. C2. 1 hit.
SM00142. PI3K_C2. 1 hit.
SM00144. PI3K_rbd. 1 hit.
SM00145. PI3Ka. 1 hit.
SM00146. PI3Kc. 1 hit.
SM00312. PX. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
SSF49562. SSF49562. 2 hits.
SSF54236. SSF54236. 1 hit.
SSF56112. SSF56112. 1 hit.
SSF64268. SSF64268. 1 hit.
PROSITEiPS50004. C2. 1 hit.
PS00915. PI3_4_KINASE_1. 1 hit.
PS00916. PI3_4_KINASE_2. 1 hit.
PS50290. PI3_4_KINASE_3. 1 hit.
PS51547. PI3K_C2. 1 hit.
PS51546. PI3K_RBD. 1 hit.
PS51545. PIK_HELICAL. 1 hit.
PS50195. PX. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiP3C2B_HUMAN
AccessioniPrimary (citable) accession number: O00750
Secondary accession number(s): O95666, Q5SW99
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: August 30, 2005
Last modified: November 2, 2016
This is version 174 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Caution

It is uncertain whether Met-1 or Met-26 is the initiator.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.