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O00744 (WN10B_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 116. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein Wnt-10b
Alternative name(s):
Protein Wnt-12
Gene names
Name:WNT10B
Synonyms:WNT12
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length389 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters By similarity.

Subcellular location

Secretedextracellular spaceextracellular matrix.

Tissue specificity

Detected in most adult tissues. Highest levels were found in heart and skeletal muscle. Low levels are found in brain.

Developmental stage

Infant brain has higher levels of WNT10B than adult brain.

Involvement in disease

Split-hand/foot malformation 6 (SHFM6) [MIM:225300]: A limb malformation involving the central rays of the autopod and presenting with syndactyly, median clefts of the hands and feet, and aplasia and/or hypoplasia of the phalanges, metacarpals, and metatarsals. Some patients have been found to have mental retardation, ectodermal and craniofacial findings, and orofacial clefting.
Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.10

Sequence similarities

Belongs to the Wnt family.

Ontologies

Keywords
   Biological processWnt signaling pathway
   Cellular componentExtracellular matrix
Secreted
   Coding sequence diversityPolymorphism
   DiseaseDisease mutation
   DomainSignal
   Molecular functionDevelopmental protein
   PTMGlycoprotein
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processG2/M transition of mitotic cell cycle

Inferred from electronic annotation. Source: Ensembl

Wnt signaling pathway

Inferred from mutant phenotype PubMed 15135146. Source: UniProtKB

bone trabecula formation

Inferred from electronic annotation. Source: Ensembl

canonical Wnt signaling pathway

Inferred from direct assay PubMed 17761539. Source: BHF-UCL

cell cycle arrest

Inferred from electronic annotation. Source: Ensembl

cell fate commitment

Inferred from Biological aspect of Ancestor. Source: RefGenome

cellular response to cAMP

Inferred from electronic annotation. Source: Ensembl

cellular response to hydrostatic pressure

Inferred from electronic annotation. Source: Ensembl

cellular response to parathyroid hormone stimulus

Inferred from electronic annotation. Source: Ensembl

cellular response to retinoic acid

Inferred from sequence or structural similarity. Source: UniProtKB

chondrocyte differentiation

Inferred from expression pattern PubMed 15135146. Source: UniProtKB

fungiform papilla development

Inferred from electronic annotation. Source: Ensembl

hematopoietic stem cell proliferation

Inferred from direct assay PubMed 9787155. Source: BHF-UCL

lipid metabolic process

Inferred from electronic annotation. Source: Ensembl

myoblast differentiation involved in skeletal muscle regeneration

Inferred from electronic annotation. Source: Ensembl

negative regulation of epithelial cell proliferation

Inferred from electronic annotation. Source: Ensembl

negative regulation of fat cell differentiation

Inferred from direct assay PubMed 10937998. Source: BHF-UCL

negative regulation of transcription from RNA polymerase II promoter

Inferred from electronic annotation. Source: Ensembl

neuron differentiation

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of G2/M transition of mitotic cell cycle

Inferred from electronic annotation. Source: Ensembl

positive regulation of RNA polymerase II transcriptional preinitiation complex assembly

Inferred from electronic annotation. Source: Ensembl

positive regulation of anagen

Inferred from electronic annotation. Source: Ensembl

positive regulation of apoptotic process

Inferred from mutant phenotype PubMed 17761539. Source: BHF-UCL

positive regulation of bone mineralization

Inferred from electronic annotation. Source: Ensembl

positive regulation of canonical Wnt signaling pathway

Inferred from electronic annotation. Source: Ensembl

positive regulation of cell proliferation

Inferred from electronic annotation. Source: Ensembl

positive regulation of epithelial cell differentiation

Inferred from electronic annotation. Source: Ensembl

positive regulation of osteoblast differentiation

Inferred from electronic annotation. Source: Ensembl

positive regulation of sequence-specific DNA binding transcription factor activity

Inferred from electronic annotation. Source: Ensembl

protein stabilization

Inferred from direct assay PubMed 10937998. Source: BHF-UCL

regulation of proteasomal ubiquitin-dependent protein catabolic process

Inferred from electronic annotation. Source: Ensembl

regulation of skeletal muscle tissue development

Inferred from electronic annotation. Source: Ensembl

sensory perception of taste

Inferred from electronic annotation. Source: Ensembl

skeletal muscle fiber development

Inferred from electronic annotation. Source: Ensembl

smoothened signaling pathway

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentextracellular region

Traceable author statement. Source: Reactome

extracellular space

Non-traceable author statement PubMed 15135146. Source: UniProtKB

proteinaceous extracellular matrix

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionfrizzled binding

Inferred from Biological aspect of Ancestor. Source: RefGenome

receptor agonist activity

Inferred by curator PubMed 17761539. Source: BHF-UCL

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2828 Potential
Chain29 – 389361Protein Wnt-10b
PRO_0000041463

Amino acid modifications

Modified residue461Phosphothreonine Ref.8
Glycosylation931N-linked (GlcNAc...) Potential
Glycosylation3351N-linked (GlcNAc...) Potential

Natural variations

Natural variant771H → Y. Ref.9
Corresponds to variant rs151284263 [ dbSNP | Ensembl ].
VAR_062512
Natural variant2561C → Y Associated with obesity; abrogates the ability of WNT10B to activate canonical Wnt signalling and blocks adipogenesis. Ref.9
VAR_062513
Natural variant2851I → T. Ref.9
VAR_062514
Natural variant3011P → S. Ref.9
Corresponds to variant rs35034312 [ dbSNP | Ensembl ].
VAR_062515
Natural variant3321R → W in SHFM6. Ref.10
VAR_062516

Experimental info

Sequence conflict601G → D in AAB51685. Ref.1
Sequence conflict1491K → R in CAA65769. Ref.6
Sequence conflict2951F → S in CAA65769. Ref.6
Sequence conflict3111F → L in CAA65769. Ref.6

Sequences

Sequence LengthMass (Da)Tools
O00744 [UniParc].

Last modified May 10, 2002. Version 2.
Checksum: F973F2CA0DB115EF

FASTA38943,000
        10         20         30         40         50         60 
MLEEPRPRPP PSGLAGLLFL ALCSRALSNE ILGLKLPGEP PLTANTVCLT LSGLSKRQLG 

        70         80         90        100        110        120 
LCLRNPDVTA SALQGLHIAV HECQHQLRDQ RWNCSALEGG GRLPHHSAIL KRGFRESAFS 

       130        140        150        160        170        180 
FSMLAAGVMH AVATACSLGK LVSCGCGWKG SGEQDRLRAK LLQLQALSRG KSFPHSLPSP 

       190        200        210        220        230        240 
GPGSSPSPGP QDTWEWGGCN HDMDFGEKFS RDFLDSREAP RDIQARMRIH NNRVGRQVVT 

       250        260        270        280        290        300 
ENLKRKCKCH GTSGSCQFKT CWRAAPEFRA VGAALRERLG RAIFIDTHNR NSGAFQPRLR 

       310        320        330        340        350        360 
PRRLSGELVY FEKSPDFCER DPTMGSPGTR GRACNKTSRL LDGCGSLCCG RGHNVLRQTR 

       370        380 
VERCHCRFHW CCYVLCDECK VTEWVNVCK 

« Hide

References

« Hide 'large scale' references
[1]"Isolation, characterization and chromosomal localization of human WNT10B."
Hardiman G., Kastelein R.A., Bazan J.F.
Cytogenet. Cell Genet. 77:278-282(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Proto-oncogene WNT10B is up-regulated by tumor necrosis factor alpha in human gastric cancer cell line MKN45."
Saitoh T., Kirikoshi H., Mine T., Katoh M.
Int. J. Oncol. 19:1187-1192(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Subthalamic nucleus.
[4]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]"A novel human Wnt gene, WNT10B, maps to 12q13 and is expressed in human breast carcinomas."
Bui T.D., Rankin J., Smith K., Huguet E.L., Ruben S., Strachan T., Harris A.L., Lindsay S.
Oncogene 14:1249-1253(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 45-347.
Tissue: Fetal brain.
[7]"Isolation of two novel WNT genes, WNT14 and WNT15, one of which (WNT15) is closely linked to WNT3 on human chromosome 17q21."
Bergstein I., Eisenberg L.M., Bhalerao J., Jenkins N.A., Copeland N.G., Osborne M.P., Bowcock A.M., Brown A.M.C.
Genomics 46:450-458(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 253-368.
Tissue: Placenta.
[8]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-46, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[9]"WNT10B mutations in human obesity."
Christodoulides C., Scarda A., Granzotto M., Milan G., Dalla Nora E., Keogh J., De Pergola G., Stirling H., Pannacciulli N., Sethi J.K., Federspil G., Vidal-Puig A., Farooqi I.S., O'Rahilly S., Vettor R.
Diabetologia 49:678-684(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANTS TYR-77; TYR-256; THR-285 AND SER-301, ASSOCIATION OF VARIANT TYR-256 WITH OBESITY.
[10]"Homozygous WNT10b mutation and complex inheritance in Split-Hand/Foot Malformation."
Ugur S.A., Tolun A.
Hum. Mol. Genet. 17:2644-2653(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANT SHFM6 TRP-332.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U81787 mRNA. Translation: AAB51685.1.
AB070724 mRNA. Translation: BAB72181.1.
AK312906 mRNA. Translation: BAG35752.1.
CH471111 Genomic DNA. Translation: EAW58028.1.
BC096353 mRNA. Translation: AAH96353.1.
BC096354 mRNA. Translation: AAH96354.1.
BC096356 mRNA. Translation: AAH96356.1.
X97057 mRNA. Translation: CAA65769.1.
AF028700 Genomic DNA. Translation: AAC39549.1.
RefSeqNP_003385.2. NM_003394.3.
UniGeneHs.91985.

3D structure databases

ProteinModelPortalO00744.
SMRO00744. Positions 69-380.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid113317. 1 interaction.
STRING9606.ENSP00000301061.

PTM databases

PhosphoSiteO00744.

Proteomic databases

PaxDbO00744.
PRIDEO00744.

Protocols and materials databases

DNASU7480.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000301061; ENSP00000301061; ENSG00000169884.
GeneID7480.
KEGGhsa:7480.
UCSCuc001rss.3. human.

Organism-specific databases

CTD7480.
GeneCardsGC12M049359.
HGNCHGNC:12775. WNT10B.
HPAHPA055048.
MIM225300. phenotype.
601906. gene.
neXtProtNX_O00744.
Orphanet2440. Split hand-split foot malformation.
PharmGKBPA37377.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG240772.
HOGENOMHOG000039528.
HOVERGENHBG001595.
InParanoidO00744.
KOK01357.
OMASERCNCR.
OrthoDBEOG7C8GJ8.
PhylomeDBO00744.
TreeFamTF105310.

Enzyme and pathway databases

ReactomeREACT_111102. Signal Transduction.
SignaLinkO00744.

Gene expression databases

ArrayExpressO00744.
BgeeO00744.
CleanExHS_WNT10B.
GenevestigatorO00744.

Family and domain databases

InterProIPR005817. Wnt.
IPR013302. Wnt10.
IPR018161. Wnt_CS.
[Graphical view]
PANTHERPTHR12027. PTHR12027. 1 hit.
PTHR12027:SF11. PTHR12027:SF11. 1 hit.
PfamPF00110. wnt. 1 hit.
[Graphical view]
PRINTSPR01893. WNT10PROTEIN.
PR01349. WNTPROTEIN.
SMARTSM00097. WNT1. 1 hit.
[Graphical view]
PROSITEPS00246. WNT1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiWNT10B.
GenomeRNAi7480.
NextBio29300.
PROO00744.
SOURCESearch...

Entry information

Entry nameWN10B_HUMAN
AccessionPrimary (citable) accession number: O00744
Secondary accession number(s): B2R7A5 expand/collapse secondary AC list , O00747, Q4VAJ5, Q8WZ97
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: May 10, 2002
Last modified: April 16, 2014
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 12

Human chromosome 12: entries, gene names and cross-references to MIM