O00744 (WN10B_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein Wnt-10b

Alternative name(s):
Protein Wnt-12

Gene names

Name:	WNT10B
Synonyms:	WNT12

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	389 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters By similarity.
Subcellular location	Secreted › extracellular space › extracellular matrix.
Tissue specificity	Detected in most adult tissues. Highest levels were found in heart and skeletal muscle. Low levels are found in brain.
Developmental stage	Infant brain has higher levels of WNT10B than adult brain.
Involvement in disease	Split-hand/foot malformation 6 (SHFM6) [MIM:225300]: A limb malformation involving the central rays of the autopod and presenting with syndactyly, median clefts of the hands and feet, and aplasia and/or hypoplasia of the phalanges, metacarpals, and metatarsals. Some patients have been found to have mental retardation, ectodermal and craniofacial findings, and orofacial clefting. Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.10
Sequence similarities	Belongs to the Wnt family.

Ontologies

Keywords
Biological process	Wnt signaling pathway
Cellular component	Extracellular matrix Secreted
Coding sequence diversity	Polymorphism
Disease	Disease mutation
Domain	Signal
Molecular function	Developmental protein
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	G2/M transition of mitotic cell cycle Inferred from electronic annotation. Source: Compara axis specification Inferred from Biological aspect of Ancestor. Source: RefGenome bone trabecula formation Inferred from Biological aspect of Ancestor. Source: RefGenome canonical Wnt receptor signaling pathway Inferred from direct assay PubMed 17761539. Source: BHF-UCL cell cycle arrest Inferred from electronic annotation. Source: Compara cellular response to cAMP Inferred from electronic annotation. Source: Compara cellular response to parathyroid hormone stimulus Inferred from electronic annotation. Source: Compara cellular response to retinoic acid Inferred from sequence or structural similarity. Source: UniProtKB chondrocyte differentiation Inferred from expression pattern PubMed 15135146. Source: UniProtKB female gonad development Inferred from Biological aspect of Ancestor. Source: RefGenome fungiform papilla development Inferred from electronic annotation. Source: Compara hematopoietic stem cell proliferation Inferred from direct assay PubMed 9787155. Source: BHF-UCL lipid metabolic process Inferred from electronic annotation. Source: Compara midbrain-hindbrain boundary development Inferred from Biological aspect of Ancestor. Source: RefGenome myoblast cell differentiation involved in skeletal muscle regeneration Inferred from Biological aspect of Ancestor. Source: RefGenome negative regulation of epithelial cell proliferation Inferred from Biological aspect of Ancestor. Source: RefGenome negative regulation of fat cell differentiation Inferred from direct assay PubMed 10937998. Source: BHF-UCL negative regulation of transcription from RNA polymerase II promoter Inferred from electronic annotation. Source: Compara neuron differentiation Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of G2/M transition of mitotic cell cycle Inferred from electronic annotation. Source: Compara positive regulation of RNA polymerase II transcriptional preinitiation complex assembly Inferred from electronic annotation. Source: Compara positive regulation of anagen Inferred from Biological aspect of Ancestor. Source: RefGenome positive regulation of apoptotic process Inferred from mutant phenotype PubMed 17761539. Source: BHF-UCL positive regulation of bone mineralization Inferred from Biological aspect of Ancestor. Source: RefGenome positive regulation of canonical Wnt receptor signaling pathway Inferred from electronic annotation. Source: Compara positive regulation of cell proliferation Inferred from Biological aspect of Ancestor. Source: RefGenome positive regulation of epithelial cell differentiation Inferred from Biological aspect of Ancestor. Source: RefGenome positive regulation of osteoblast differentiation Inferred from Biological aspect of Ancestor. Source: RefGenome positive regulation of sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: Compara protein stabilization Inferred from direct assay PubMed 10937998. Source: BHF-UCL regulation of proteasomal ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: Compara regulation of skeletal muscle tissue development Inferred from Biological aspect of Ancestor. Source: RefGenome sensory perception of taste Inferred from electronic annotation. Source: Compara skeletal muscle fiber development Inferred from Biological aspect of Ancestor. Source: RefGenome smoothened signaling pathway Inferred from Biological aspect of Ancestor. Source: RefGenome tongue development Inferred from Biological aspect of Ancestor. Source: RefGenome
Cellular_component	extracellular space Non-traceable author statement PubMed 15135146. Source: UniProtKB plasma membrane Inferred from Biological aspect of Ancestor. Source: RefGenome proteinaceous extracellular matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	G-protein coupled receptor binding Inferred from Biological aspect of Ancestor. Source: RefGenome receptor agonist activity Inferred by curator PubMed 17761539. Source: BHF-UCL
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 28	28	Potential
	Chain	29 – 389	361	Protein Wnt-10b		PRO_0000041463
Amino acid modifications
	Modified residue	46	1	Phosphothreonine Ref.8
	Glycosylation	93	1	N-linked (GlcNAc...) Potential
	Glycosylation	335	1	N-linked (GlcNAc...) Potential
Natural variations
	Natural variant	77	1	H → Y. Ref.9		VAR_062512
	Natural variant	256	1	C → Y Associated with obesity; abrogates the ability of WNT10B to activate canonical Wnt signalling and blocks adipogenesis. Ref.9		VAR_062513
	Natural variant	285	1	I → T. Ref.9		VAR_062514
	Natural variant	301	1	P → S. Ref.9		VAR_062515
	Natural variant	332	1	R → W in SHFM6. Ref.10		VAR_062516
Experimental info
	Sequence conflict	60	1	G → D in AAB51685. Ref.1
	Sequence conflict	149	1	K → R in CAA65769. Ref.6
	Sequence conflict	295	1	F → S in CAA65769. Ref.6
	Sequence conflict	311	1	F → L in CAA65769. Ref.6

Sequences

	Sequence		Length	Mass (Da)
	O00744 [UniParc]. Last modified May 10, 2002. Version 2. Checksum: F973F2CA0DB115EF	FASTA	389	43,000
	10 20 30 40 50 60 MLEEPRPRPP PSGLAGLLFL ALCSRALSNE ILGLKLPGEP PLTANTVCLT LSGLSKRQLG 70 80 90 100 110 120 LCLRNPDVTA SALQGLHIAV HECQHQLRDQ RWNCSALEGG GRLPHHSAIL KRGFRESAFS 130 140 150 160 170 180 FSMLAAGVMH AVATACSLGK LVSCGCGWKG SGEQDRLRAK LLQLQALSRG KSFPHSLPSP 190 200 210 220 230 240 GPGSSPSPGP QDTWEWGGCN HDMDFGEKFS RDFLDSREAP RDIQARMRIH NNRVGRQVVT 250 260 270 280 290 300 ENLKRKCKCH GTSGSCQFKT CWRAAPEFRA VGAALRERLG RAIFIDTHNR NSGAFQPRLR 310 320 330 340 350 360 PRRLSGELVY FEKSPDFCER DPTMGSPGTR GRACNKTSRL LDGCGSLCCG RGHNVLRQTR 370 380 VERCHCRFHW CCYVLCDECK VTEWVNVCK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation, characterization and chromosomal localization of human WNT10B." Hardiman G., Kastelein R.A., Bazan J.F. Cytogenet. Cell Genet. 77:278-282(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Proto-oncogene WNT10B is up-regulated by tumor necrosis factor alpha in human gastric cancer cell line MKN45." Saitoh T., Kirikoshi H., Mine T., Katoh M. Int. J. Oncol. 19:1187-1192(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Subthalamic nucleus.
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]	"A novel human Wnt gene, WNT10B, maps to 12q13 and is expressed in human breast carcinomas." Bui T.D., Rankin J., Smith K., Huguet E.L., Ruben S., Strachan T., Harris A.L., Lindsay S. Oncogene 14:1249-1253(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 45-347. Tissue: Fetal brain.
[7]	"Isolation of two novel WNT genes, WNT14 and WNT15, one of which (WNT15) is closely linked to WNT3 on human chromosome 17q21." Bergstein I., Eisenberg L.M., Bhalerao J., Jenkins N.A., Copeland N.G., Osborne M.P., Bowcock A.M., Brown A.M.C. Genomics 46:450-458(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 253-368. Tissue: Placenta.
[8]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-46, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[9]	"WNT10B mutations in human obesity." Christodoulides C., Scarda A., Granzotto M., Milan G., Dalla Nora E., Keogh J., De Pergola G., Stirling H., Pannacciulli N., Sethi J.K., Federspil G., Vidal-Puig A., Farooqi I.S., O'Rahilly S., Vettor R. Diabetologia 49:678-684(2006) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANTS TYR-77; TYR-256; THR-285 AND SER-301, ASSOCIATION OF VARIANT TYR-256 WITH OBESITY.
[10]	"Homozygous WNT10b mutation and complex inheritance in Split-Hand/Foot Malformation." Ugur S.A., Tolun A. Hum. Mol. Genet. 17:2644-2653(2008) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANT SHFM6 TRP-332.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U81787 mRNA. Translation: AAB51685.1. AB070724 mRNA. Translation: BAB72181.1. AK312906 mRNA. Translation: BAG35752.1. CH471111 Genomic DNA. Translation: EAW58028.1. BC096353 mRNA. Translation: AAH96353.1. BC096354 mRNA. Translation: AAH96354.1. BC096356 mRNA. Translation: AAH96356.1. X97057 mRNA. Translation: CAA65769.1. AF028700 Genomic DNA. Translation: AAC39549.1.
IPI	IPI00012971.
RefSeq	NP_003385.2. NM_003394.3.
UniGene	Hs.91985.
3D structure databases
ProteinModelPortal	O00744.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000301061.
PTM databases
PhosphoSite	O00744.
Proteomic databases
PaxDb	O00744.
PRIDE	O00744.
Protocols and materials databases
DNASU	7480.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000301061; ENSP00000301061; ENSG00000169884.
GeneID	7480.
KEGG	hsa:7480.
UCSC	uc001rss.3. human.
Organism-specific databases
CTD	7480.
GeneCards	GC12M049359.
HGNC	HGNC:12775. WNT10B.
HPA	HPA055048.
MIM	225300. phenotype. 601906. gene.
neXtProt	NX_O00744.
Orphanet	2440. Split hand-split foot malformation.
PharmGKB	PA37377.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG240772.
HOGENOM	HOG000039528.
HOVERGEN	HBG001595.
InParanoid	O00744.
KO	K01357.
OMA	SERCNCR.
OrthoDB	EOG4B8JD8.
PhylomeDB	O00744.
Enzyme and pathway databases
Reactome	REACT_111102. Signal Transduction.
Gene expression databases
ArrayExpress	O00744.
Bgee	O00744.
CleanEx	HS_WNT10B.
Genevestigator	O00744.
GermOnline	ENSG00000169884. Homo sapiens.
Family and domain databases
InterPro	IPR005817. Wnt. IPR013302. Wnt10. IPR018161. Wnt_CS. [Graphical view]
PANTHER	PTHR12027. PTHR12027. 1 hit. PTHR12027:SF11. PTHR12027:SF11. 1 hit.
Pfam	PF00110. wnt. 1 hit. [Graphical view]
PRINTS	PR01893. WNT10PROTEIN. PR01349. WNTPROTEIN.
SMART	SM00097. WNT1. 1 hit. [Graphical view]
PROSITE	PS00246. WNT1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	7480.
NextBio	29300.
SOURCE	Search...

Entry information

Entry name

WN10B_HUMAN

Accession

Primary (citable) accession number: O00744
Secondary accession number(s): B2R7A5 , O00747, Q4VAJ5, Q8WZ97

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	May 10, 2002
Last modified:	May 1, 2013
This is version 107 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.