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O00716

- E2F3_HUMAN

UniProt

O00716 - E2F3_HUMAN

Protein

Transcription factor E2F3

Gene

E2F3

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 140 (01 Oct 2014)
      Sequence version 1 (01 Jul 1997)
      Previous versions | rss
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    Functioni

    Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F3 binds specifically to RB1 in a cell-cycle dependent manner.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi155 – 24591Sequence AnalysisAdd
    BLAST

    GO - Molecular functioni

    1. core promoter binding Source: UniProtKB
    2. protein binding Source: UniProtKB
    3. sequence-specific DNA binding transcription factor activity Source: ProtInc

    GO - Biological processi

    1. mitotic cell cycle Source: Reactome
    2. mitotic G2 phase Source: Reactome
    3. Notch signaling pathway Source: Reactome
    4. positive regulation of cell proliferation Source: Ensembl
    5. positive regulation of transcription, DNA-templated Source: Ensembl
    6. transcription initiation from RNA polymerase II promoter Source: ProtInc

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Cell cycle, Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    ReactomeiREACT_1181. Association of licensing factors with the pre-replicative complex.
    REACT_118568. Pre-NOTCH Transcription and Translation.
    REACT_169325. Oncogene Induced Senescence.
    REACT_169436. Oxidative Stress Induced Senescence.
    REACT_1707. CDC6 association with the ORC:origin complex.
    REACT_1915. G2 Phase.
    REACT_821. Cyclin D associated events in G1.
    SignaLinkiO00716.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Transcription factor E2F3
    Short name:
    E2F-3
    Gene namesi
    Name:E2F3
    Synonyms:KIAA0075
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 6

    Organism-specific databases

    HGNCiHGNC:3115. E2F3.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: HPA
    2. Golgi apparatus Source: HPA
    3. intracellular membrane-bounded organelle Source: HPA
    4. nucleoplasm Source: Reactome
    5. nucleus Source: HPA
    6. transcription factor complex Source: InterPro

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA27573.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 465465Transcription factor E2F3PRO_0000219466Add
    BLAST

    Proteomic databases

    MaxQBiO00716.
    PaxDbiO00716.
    PRIDEiO00716.

    PTM databases

    PhosphoSiteiO00716.

    Expressioni

    Gene expression databases

    ArrayExpressiO00716.
    BgeeiO00716.
    CleanExiHS_E2F3.
    GenevestigatoriO00716.

    Organism-specific databases

    HPAiHPA051453.
    HPA053566.

    Interactioni

    Subunit structurei

    Component of the DRTF1/E2F transcription factor complex. Binds cooperatively with TFDP1/Dp-1 to E2F sites. Interacts with retinoblastoma protein RB1 and related proteins (such as RBL1) that inhibit the E2F transactivation domain. Binds EAPP.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    HELLSQ9NRZ92EBI-765551,EBI-1056215
    MSH2P432462EBI-765551,EBI-355888
    RB1P064004EBI-765551,EBI-491274

    Protein-protein interaction databases

    BioGridi108203. 29 interactions.
    DIPiDIP-24230N.
    IntActiO00716. 125 interactions.
    MINTiMINT-249699.
    STRINGi9606.ENSP00000262904.

    Structurei

    3D structure databases

    ProteinModelPortaliO00716.
    SMRiO00716. Positions 177-241, 257-354.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni101 – 15353Cyclin A/CDK2 bindingSequence AnalysisAdd
    BLAST
    Regioni204 – 22522Leucine-zipperAdd
    BLAST
    Regioni246 – 33792DimerizationSequence AnalysisAdd
    BLAST
    Regioni391 – 46575TransactivationSequence AnalysisAdd
    BLAST
    Regioni432 – 44918Retinoblastoma protein bindingSequence AnalysisAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi209 – 24537DEF boxAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi26 – 316Poly-Ala
    Compositional biasi45 – 539Poly-Ala
    Compositional biasi120 – 12910Poly-Gly

    Sequence similaritiesi

    Belongs to the E2F/DP family.Curated

    Phylogenomic databases

    eggNOGiNOG289227.
    HOGENOMiHOG000232045.
    HOVERGENiHBG002227.
    InParanoidiO00716.
    KOiK06620.
    OMAiLLYTTPH.
    OrthoDBiEOG738058.
    PhylomeDBiO00716.
    TreeFamiTF105566.

    Family and domain databases

    Gene3Di1.10.10.10. 1 hit.
    InterProiIPR015633. E2F.
    IPR003316. E2F_TDP.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    PANTHERiPTHR12081. PTHR12081. 1 hit.
    PfamiPF02319. E2F_TDP. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: O00716-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MRKGIQPALE QYLVTAGGGE GAAVVAAAAA ASMDKRALLA SPGFAAAAAA    50
    AAAPGAYIQI LTTNTSTTSC SSSLQSGAVA AGPLLPSAPG AEQTAGSLLY 100
    TTPHGPSSRA GLLQQPPALG RGGSGGGGGP PAKRRLELGE SGHQYLSDGL 150
    KTPKGKGRAA LRSPDSPKTP KSPSEKTRYD TSLGLLTKKF IQLLSQSPDG 200
    VLDLNKAAEV LKVQKRRIYD ITNVLEGIHL IKKKSKNNVQ WMGCSLSEDG 250
    GMLAQCQGLS KEVTELSQEE KKLDELIQSC TLDLKLLTED SENQRLAYVT 300
    YQDIRKISGL KDQTVIVVKA PPETRLEVPD SIESLQIHLA STQGPIEVYL 350
    CPEETETHSP MKTNNQDHNG NIPKPASKDL ASTNSGHSDC SVSMGNLSPL 400
    ASPANLLQQT EDQIPSNLEG PFVNLLPPLL QEDYLLSLGE EEGISDLFDA 450
    YDLEKLPLVE DFMCS 465
    Length:465
    Mass (Da):49,162
    Last modified:July 1, 1997 - v1
    Checksum:i4641565842CA99EC
    GO
    Isoform 2 (identifier: O00716-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-6: MRKGIQ → MPLQQQ
         7-131: Missing.
         169-175: TPKSPSE → K

    Note: No experimental confirmation available.

    Show »
    Length:334
    Mass (Da):37,010
    Checksum:i11CD170783B1974D
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti344 – 3441G → R.1 Publication
    Corresponds to variant rs4134973 [ dbSNP | Ensembl ].
    VAR_014341
    Natural varianti389 – 3891D → N.1 Publication
    Corresponds to variant rs4134982 [ dbSNP | Ensembl ].
    VAR_014342

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 66MRKGIQ → MPLQQQ in isoform 2. 1 PublicationVSP_045066
    Alternative sequencei7 – 131125Missing in isoform 2. 1 PublicationVSP_045067Add
    BLAST
    Alternative sequencei169 – 1757TPKSPSE → K in isoform 2. 1 PublicationVSP_045068

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y10479 mRNA. Translation: CAA71504.1.
    AF547386 Genomic DNA. Translation: AAN17846.1.
    CR749285 mRNA. Translation: CAH18140.1.
    AL132775, AL136303 Genomic DNA. Translation: CAI21471.1.
    AL136303 Genomic DNA. Translation: CAC28598.2.
    D38550 mRNA. Translation: BAA07553.1.
    CCDSiCCDS4545.1. [O00716-1]
    CCDS58999.1. [O00716-2]
    RefSeqiNP_001230005.1. NM_001243076.2. [O00716-2]
    NP_001940.1. NM_001949.4. [O00716-1]
    UniGeneiHs.269408.
    Hs.703174.

    Genome annotation databases

    EnsembliENST00000346618; ENSP00000262904; ENSG00000112242. [O00716-1]
    ENST00000535432; ENSP00000443418; ENSG00000112242. [O00716-2]
    GeneIDi1871.
    KEGGihsa:1871.
    UCSCiuc003ncz.2. human. [O00716-1]
    uc021ymj.2. human.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Web resourcesi

    Atlas of Genetics and Cytogenetics in Oncology and Haematology
    NIEHS-SNPs

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y10479 mRNA. Translation: CAA71504.1 .
    AF547386 Genomic DNA. Translation: AAN17846.1 .
    CR749285 mRNA. Translation: CAH18140.1 .
    AL132775 , AL136303 Genomic DNA. Translation: CAI21471.1 .
    AL136303 Genomic DNA. Translation: CAC28598.2 .
    D38550 mRNA. Translation: BAA07553.1 .
    CCDSi CCDS4545.1. [O00716-1 ]
    CCDS58999.1. [O00716-2 ]
    RefSeqi NP_001230005.1. NM_001243076.2. [O00716-2 ]
    NP_001940.1. NM_001949.4. [O00716-1 ]
    UniGenei Hs.269408.
    Hs.703174.

    3D structure databases

    ProteinModelPortali O00716.
    SMRi O00716. Positions 177-241, 257-354.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 108203. 29 interactions.
    DIPi DIP-24230N.
    IntActi O00716. 125 interactions.
    MINTi MINT-249699.
    STRINGi 9606.ENSP00000262904.

    PTM databases

    PhosphoSitei O00716.

    Proteomic databases

    MaxQBi O00716.
    PaxDbi O00716.
    PRIDEi O00716.

    Protocols and materials databases

    DNASUi 1871.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000346618 ; ENSP00000262904 ; ENSG00000112242 . [O00716-1 ]
    ENST00000535432 ; ENSP00000443418 ; ENSG00000112242 . [O00716-2 ]
    GeneIDi 1871.
    KEGGi hsa:1871.
    UCSCi uc003ncz.2. human. [O00716-1 ]
    uc021ymj.2. human.

    Organism-specific databases

    CTDi 1871.
    GeneCardsi GC06P020402.
    HGNCi HGNC:3115. E2F3.
    HPAi HPA051453.
    HPA053566.
    MIMi 600427. gene.
    neXtProti NX_O00716.
    PharmGKBi PA27573.
    HUGEi Search...
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG289227.
    HOGENOMi HOG000232045.
    HOVERGENi HBG002227.
    InParanoidi O00716.
    KOi K06620.
    OMAi LLYTTPH.
    OrthoDBi EOG738058.
    PhylomeDBi O00716.
    TreeFami TF105566.

    Enzyme and pathway databases

    Reactomei REACT_1181. Association of licensing factors with the pre-replicative complex.
    REACT_118568. Pre-NOTCH Transcription and Translation.
    REACT_169325. Oncogene Induced Senescence.
    REACT_169436. Oxidative Stress Induced Senescence.
    REACT_1707. CDC6 association with the ORC:origin complex.
    REACT_1915. G2 Phase.
    REACT_821. Cyclin D associated events in G1.
    SignaLinki O00716.

    Miscellaneous databases

    GeneWikii E2F3.
    GenomeRNAii 1871.
    NextBioi 7651.
    PROi O00716.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O00716.
    Bgeei O00716.
    CleanExi HS_E2F3.
    Genevestigatori O00716.

    Family and domain databases

    Gene3Di 1.10.10.10. 1 hit.
    InterProi IPR015633. E2F.
    IPR003316. E2F_TDP.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    PANTHERi PTHR12081. PTHR12081. 1 hit.
    Pfami PF02319. E2F_TDP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The retinoblastoma protein binds to a family of E2F transcription factors."
      Lees J.A., Saito M., Vidal M., Valentine M., Look T., Harlow E., Dyson N., Helin K.
      Mol. Cell. Biol. 13:7813-7825(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Tissue: Pre-B cell.
    2. NIEHS SNPs program
      Submitted (SEP-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS ARG-344 AND ASN-389.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Rectum tumor.
    4. "The DNA sequence and analysis of human chromosome 6."
      Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D.
      , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S.
      Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "Prediction of the coding sequences of unidentified human genes. II. The coding sequences of 40 new genes (KIAA0041-KIAA0080) deduced by analysis of cDNA clones from human cell line KG-1."
      Nomura N., Nagase T., Miyajima N., Sazuka T., Tanaka A., Sato S., Seki N., Kawarabayasi Y., Ishikawa K., Tabata S.
      DNA Res. 1:223-229(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 292-465 (ISOFORM 1/2).
      Tissue: Bone marrow.
    6. "EAPP, a novel E2F binding protein that modulates E2F-dependent transcription."
      Novy M., Pohn R., Andorfer P., Novy-Weiland T., Galos B., Schwarzmayr L., Rotheneder H.
      Mol. Biol. Cell 16:2181-2190(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH EAPP.

    Entry informationi

    Entry nameiE2F3_HUMAN
    AccessioniPrimary (citable) accession number: O00716
    Secondary accession number(s): Q15000, Q68DT0, Q9BZ44
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: July 1, 1997
    Last modified: October 1, 2014
    This is version 140 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 6
      Human chromosome 6: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3