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O00716 (E2F3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 135. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Transcription factor E2F3

Short name=E2F-3
Gene names
Name:E2F3
Synonyms:KIAA0075
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length465 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F3 binds specifically to RB1 in a cell-cycle dependent manner.

Subunit structure

Component of the DRTF1/E2F transcription factor complex. Binds cooperatively with TFDP1/Dp-1 to E2F sites. Interacts with retinoblastoma protein RB1 and related proteins (such as RBL1) that inhibit the E2F transactivation domain. Binds EAPP. Ref.6

Subcellular location

Nucleus.

Sequence similarities

Belongs to the E2F/DP family.

Binary interactions

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: O00716-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: O00716-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-6: MRKGIQ → MPLQQQ
     7-131: Missing.
     169-175: TPKSPSE → K
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 465465Transcription factor E2F3
PRO_0000219466

Regions

DNA binding155 – 24591 Potential
Region101 – 15353Cyclin A/CDK2 binding Potential
Region204 – 22522Leucine-zipper
Region246 – 33792Dimerization Potential
Region391 – 46575Transactivation Potential
Region432 – 44918Retinoblastoma protein binding Potential
Motif209 – 24537DEF box
Compositional bias26 – 316Poly-Ala
Compositional bias45 – 539Poly-Ala
Compositional bias120 – 12910Poly-Gly

Natural variations

Alternative sequence1 – 66MRKGIQ → MPLQQQ in isoform 2.
VSP_045066
Alternative sequence7 – 131125Missing in isoform 2.
VSP_045067
Alternative sequence169 – 1757TPKSPSE → K in isoform 2.
VSP_045068
Natural variant3441G → R. Ref.2
Corresponds to variant rs4134973 [ dbSNP | Ensembl ].
VAR_014341
Natural variant3891D → N. Ref.2
Corresponds to variant rs4134982 [ dbSNP | Ensembl ].
VAR_014342

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified July 1, 1997. Version 1.
Checksum: 4641565842CA99EC

FASTA46549,162
        10         20         30         40         50         60 
MRKGIQPALE QYLVTAGGGE GAAVVAAAAA ASMDKRALLA SPGFAAAAAA AAAPGAYIQI 

        70         80         90        100        110        120 
LTTNTSTTSC SSSLQSGAVA AGPLLPSAPG AEQTAGSLLY TTPHGPSSRA GLLQQPPALG 

       130        140        150        160        170        180 
RGGSGGGGGP PAKRRLELGE SGHQYLSDGL KTPKGKGRAA LRSPDSPKTP KSPSEKTRYD 

       190        200        210        220        230        240 
TSLGLLTKKF IQLLSQSPDG VLDLNKAAEV LKVQKRRIYD ITNVLEGIHL IKKKSKNNVQ 

       250        260        270        280        290        300 
WMGCSLSEDG GMLAQCQGLS KEVTELSQEE KKLDELIQSC TLDLKLLTED SENQRLAYVT 

       310        320        330        340        350        360 
YQDIRKISGL KDQTVIVVKA PPETRLEVPD SIESLQIHLA STQGPIEVYL CPEETETHSP 

       370        380        390        400        410        420 
MKTNNQDHNG NIPKPASKDL ASTNSGHSDC SVSMGNLSPL ASPANLLQQT EDQIPSNLEG 

       430        440        450        460 
PFVNLLPPLL QEDYLLSLGE EEGISDLFDA YDLEKLPLVE DFMCS 

« Hide

Isoform 2 [UniParc].

Checksum: 11CD170783B1974D
Show »

FASTA33437,010

References

« Hide 'large scale' references
[1]"The retinoblastoma protein binds to a family of E2F transcription factors."
Lees J.A., Saito M., Vidal M., Valentine M., Look T., Harlow E., Dyson N., Helin K.
Mol. Cell. Biol. 13:7813-7825(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Pre-B cell.
[2]NIEHS SNPs program
Submitted (SEP-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS ARG-344 AND ASN-389.
[3]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Rectum tumor.
[4]"The DNA sequence and analysis of human chromosome 6."
Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. expand/collapse author list , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S.
Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"Prediction of the coding sequences of unidentified human genes. II. The coding sequences of 40 new genes (KIAA0041-KIAA0080) deduced by analysis of cDNA clones from human cell line KG-1."
Nomura N., Nagase T., Miyajima N., Sazuka T., Tanaka A., Sato S., Seki N., Kawarabayasi Y., Ishikawa K., Tabata S.
DNA Res. 1:223-229(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 292-465 (ISOFORM 1/2).
Tissue: Bone marrow.
[6]"EAPP, a novel E2F binding protein that modulates E2F-dependent transcription."
Novy M., Pohn R., Andorfer P., Novy-Weiland T., Galos B., Schwarzmayr L., Rotheneder H.
Mol. Biol. Cell 16:2181-2190(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH EAPP.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Y10479 mRNA. Translation: CAA71504.1.
AF547386 Genomic DNA. Translation: AAN17846.1.
CR749285 mRNA. Translation: CAH18140.1.
AL132775, AL136303 Genomic DNA. Translation: CAI21471.1.
AL136303 Genomic DNA. Translation: CAC28598.2.
D38550 mRNA. Translation: BAA07553.1.
RefSeqNP_001230005.1. NM_001243076.2.
NP_001940.1. NM_001949.4.
UniGeneHs.269408.
Hs.703174.

3D structure databases

ProteinModelPortalO00716.
SMRO00716. Positions 177-241, 257-354.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid108203. 29 interactions.
DIPDIP-24230N.
IntActO00716. 125 interactions.
MINTMINT-249699.
STRING9606.ENSP00000262904.

PTM databases

PhosphoSiteO00716.

Proteomic databases

PaxDbO00716.
PRIDEO00716.

Protocols and materials databases

DNASU1871.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000346618; ENSP00000262904; ENSG00000112242. [O00716-1]
ENST00000535432; ENSP00000443418; ENSG00000112242. [O00716-2]
GeneID1871.
KEGGhsa:1871.
UCSCuc003ncz.2. human. [O00716-1]
uc021ymj.2. human.

Organism-specific databases

CTD1871.
GeneCardsGC06P020402.
HGNCHGNC:3115. E2F3.
HPAHPA051453.
HPA053566.
MIM600427. gene.
neXtProtNX_O00716.
PharmGKBPA27573.
HUGESearch...
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG289227.
HOGENOMHOG000232045.
HOVERGENHBG002227.
InParanoidO00716.
KOK06620.
OMALLYTTPH.
OrthoDBEOG738058.
PhylomeDBO00716.
TreeFamTF105566.

Enzyme and pathway databases

ReactomeREACT_111102. Signal Transduction.
REACT_115566. Cell Cycle.
REACT_120956. Cellular responses to stress.
SignaLinkO00716.

Gene expression databases

ArrayExpressO00716.
BgeeO00716.
CleanExHS_E2F3.
GenevestigatorO00716.

Family and domain databases

Gene3D1.10.10.10. 1 hit.
InterProIPR015633. E2F.
IPR003316. E2F_TDP.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERPTHR12081. PTHR12081. 1 hit.
PfamPF02319. E2F_TDP. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiE2F3.
GenomeRNAi1871.
NextBio7651.
PROO00716.
SOURCESearch...

Entry information

Entry nameE2F3_HUMAN
AccessionPrimary (citable) accession number: O00716
Secondary accession number(s): Q15000, Q68DT0, Q9BZ44
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 1, 1997
Last modified: April 16, 2014
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 6

Human chromosome 6: entries, gene names and cross-references to MIM