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Protein

Transcription factor E2F3

Gene

E2F3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F3 binds specifically to RB1 in a cell-cycle dependent manner.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi155 – 24591Sequence AnalysisAdd
BLAST

GO - Molecular functioni

  1. core promoter binding Source: UniProtKB
  2. sequence-specific DNA binding transcription factor activity Source: ProtInc

GO - Biological processi

  1. mitotic cell cycle Source: Reactome
  2. Notch signaling pathway Source: Reactome
  3. positive regulation of cell proliferation Source: Ensembl
  4. positive regulation of transcription, DNA-templated Source: Ensembl
  5. transcription initiation from RNA polymerase II promoter Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Cell cycle, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiREACT_1181. Association of licensing factors with the pre-replicative complex.
REACT_118568. Pre-NOTCH Transcription and Translation.
REACT_169325. Oncogene Induced Senescence.
REACT_169436. Oxidative Stress Induced Senescence.
REACT_1707. CDC6 association with the ORC:origin complex.
REACT_1915. G2 Phase.
REACT_821. Cyclin D associated events in G1.
SignaLinkiO00716.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor E2F3
Short name:
E2F-3
Gene namesi
Name:E2F3
Synonyms:KIAA0075
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 6

Organism-specific databases

HGNCiHGNC:3115. E2F3.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: HPA
  2. Golgi apparatus Source: HPA
  3. intracellular membrane-bounded organelle Source: HPA
  4. nucleoplasm Source: HPA
  5. nucleus Source: HPA
  6. transcription factor complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA27573.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 465465Transcription factor E2F3PRO_0000219466Add
BLAST

Proteomic databases

MaxQBiO00716.
PaxDbiO00716.
PRIDEiO00716.

PTM databases

PhosphoSiteiO00716.

Expressioni

Gene expression databases

BgeeiO00716.
CleanExiHS_E2F3.
ExpressionAtlasiO00716. baseline and differential.
GenevestigatoriO00716.

Organism-specific databases

HPAiHPA051453.
HPA053566.

Interactioni

Subunit structurei

Component of the DRTF1/E2F transcription factor complex. Binds cooperatively with TFDP1/Dp-1 to E2F sites. Interacts with retinoblastoma protein RB1 and related proteins (such as RBL1) that inhibit the E2F transactivation domain. Binds EAPP.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
HELLSQ9NRZ92EBI-765551,EBI-1056215
MSH2P432462EBI-765551,EBI-355888
RB1P064004EBI-765551,EBI-491274

Protein-protein interaction databases

BioGridi108203. 31 interactions.
DIPiDIP-24230N.
IntActiO00716. 125 interactions.
MINTiMINT-249699.
STRINGi9606.ENSP00000262904.

Structurei

3D structure databases

ProteinModelPortaliO00716.
SMRiO00716. Positions 177-241, 257-354.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni101 – 15353Cyclin A/CDK2 bindingSequence AnalysisAdd
BLAST
Regioni204 – 22522Leucine-zipperAdd
BLAST
Regioni246 – 33792DimerizationSequence AnalysisAdd
BLAST
Regioni391 – 46575TransactivationSequence AnalysisAdd
BLAST
Regioni432 – 44918Retinoblastoma protein bindingSequence AnalysisAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi209 – 24537DEF boxAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi26 – 316Poly-Ala
Compositional biasi45 – 539Poly-Ala
Compositional biasi120 – 12910Poly-Gly

Sequence similaritiesi

Belongs to the E2F/DP family.Curated

Phylogenomic databases

eggNOGiNOG289227.
GeneTreeiENSGT00550000074403.
HOGENOMiHOG000232045.
HOVERGENiHBG002227.
InParanoidiO00716.
KOiK06620.
OMAiLLYTTPH.
OrthoDBiEOG738058.
PhylomeDBiO00716.
TreeFamiTF105566.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR015633. E2F.
IPR003316. E2F_TDP.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR12081. PTHR12081. 1 hit.
PfamiPF02319. E2F_TDP. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: O00716-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRKGIQPALE QYLVTAGGGE GAAVVAAAAA ASMDKRALLA SPGFAAAAAA
60 70 80 90 100
AAAPGAYIQI LTTNTSTTSC SSSLQSGAVA AGPLLPSAPG AEQTAGSLLY
110 120 130 140 150
TTPHGPSSRA GLLQQPPALG RGGSGGGGGP PAKRRLELGE SGHQYLSDGL
160 170 180 190 200
KTPKGKGRAA LRSPDSPKTP KSPSEKTRYD TSLGLLTKKF IQLLSQSPDG
210 220 230 240 250
VLDLNKAAEV LKVQKRRIYD ITNVLEGIHL IKKKSKNNVQ WMGCSLSEDG
260 270 280 290 300
GMLAQCQGLS KEVTELSQEE KKLDELIQSC TLDLKLLTED SENQRLAYVT
310 320 330 340 350
YQDIRKISGL KDQTVIVVKA PPETRLEVPD SIESLQIHLA STQGPIEVYL
360 370 380 390 400
CPEETETHSP MKTNNQDHNG NIPKPASKDL ASTNSGHSDC SVSMGNLSPL
410 420 430 440 450
ASPANLLQQT EDQIPSNLEG PFVNLLPPLL QEDYLLSLGE EEGISDLFDA
460
YDLEKLPLVE DFMCS
Length:465
Mass (Da):49,162
Last modified:July 1, 1997 - v1
Checksum:i4641565842CA99EC
GO
Isoform 2 (identifier: O00716-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-6: MRKGIQ → MPLQQQ
     7-131: Missing.
     169-175: TPKSPSE → K

Note: No experimental confirmation available.

Show »
Length:334
Mass (Da):37,010
Checksum:i11CD170783B1974D
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti344 – 3441G → R.1 Publication
Corresponds to variant rs4134973 [ dbSNP | Ensembl ].
VAR_014341
Natural varianti389 – 3891D → N.1 Publication
Corresponds to variant rs4134982 [ dbSNP | Ensembl ].
VAR_014342

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 66MRKGIQ → MPLQQQ in isoform 2. 1 PublicationVSP_045066
Alternative sequencei7 – 131125Missing in isoform 2. 1 PublicationVSP_045067Add
BLAST
Alternative sequencei169 – 1757TPKSPSE → K in isoform 2. 1 PublicationVSP_045068

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y10479 mRNA. Translation: CAA71504.1.
AF547386 Genomic DNA. Translation: AAN17846.1.
CR749285 mRNA. Translation: CAH18140.1.
AL132775, AL136303 Genomic DNA. Translation: CAI21471.1.
AL136303 Genomic DNA. Translation: CAC28598.2.
D38550 mRNA. Translation: BAA07553.1.
CCDSiCCDS4545.1. [O00716-1]
CCDS58999.1. [O00716-2]
RefSeqiNP_001230005.1. NM_001243076.2. [O00716-2]
NP_001940.1. NM_001949.4. [O00716-1]
UniGeneiHs.269408.
Hs.703174.

Genome annotation databases

EnsembliENST00000346618; ENSP00000262904; ENSG00000112242. [O00716-1]
ENST00000535432; ENSP00000443418; ENSG00000112242. [O00716-2]
GeneIDi1871.
KEGGihsa:1871.
UCSCiuc003ncz.2. human. [O00716-1]
uc021ymj.2. human.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology
NIEHS-SNPs

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y10479 mRNA. Translation: CAA71504.1.
AF547386 Genomic DNA. Translation: AAN17846.1.
CR749285 mRNA. Translation: CAH18140.1.
AL132775, AL136303 Genomic DNA. Translation: CAI21471.1.
AL136303 Genomic DNA. Translation: CAC28598.2.
D38550 mRNA. Translation: BAA07553.1.
CCDSiCCDS4545.1. [O00716-1]
CCDS58999.1. [O00716-2]
RefSeqiNP_001230005.1. NM_001243076.2. [O00716-2]
NP_001940.1. NM_001949.4. [O00716-1]
UniGeneiHs.269408.
Hs.703174.

3D structure databases

ProteinModelPortaliO00716.
SMRiO00716. Positions 177-241, 257-354.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi108203. 31 interactions.
DIPiDIP-24230N.
IntActiO00716. 125 interactions.
MINTiMINT-249699.
STRINGi9606.ENSP00000262904.

PTM databases

PhosphoSiteiO00716.

Proteomic databases

MaxQBiO00716.
PaxDbiO00716.
PRIDEiO00716.

Protocols and materials databases

DNASUi1871.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000346618; ENSP00000262904; ENSG00000112242. [O00716-1]
ENST00000535432; ENSP00000443418; ENSG00000112242. [O00716-2]
GeneIDi1871.
KEGGihsa:1871.
UCSCiuc003ncz.2. human. [O00716-1]
uc021ymj.2. human.

Organism-specific databases

CTDi1871.
GeneCardsiGC06P020402.
HGNCiHGNC:3115. E2F3.
HPAiHPA051453.
HPA053566.
MIMi600427. gene.
neXtProtiNX_O00716.
PharmGKBiPA27573.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG289227.
GeneTreeiENSGT00550000074403.
HOGENOMiHOG000232045.
HOVERGENiHBG002227.
InParanoidiO00716.
KOiK06620.
OMAiLLYTTPH.
OrthoDBiEOG738058.
PhylomeDBiO00716.
TreeFamiTF105566.

Enzyme and pathway databases

ReactomeiREACT_1181. Association of licensing factors with the pre-replicative complex.
REACT_118568. Pre-NOTCH Transcription and Translation.
REACT_169325. Oncogene Induced Senescence.
REACT_169436. Oxidative Stress Induced Senescence.
REACT_1707. CDC6 association with the ORC:origin complex.
REACT_1915. G2 Phase.
REACT_821. Cyclin D associated events in G1.
SignaLinkiO00716.

Miscellaneous databases

GeneWikiiE2F3.
GenomeRNAii1871.
NextBioi7651.
PROiO00716.
SOURCEiSearch...

Gene expression databases

BgeeiO00716.
CleanExiHS_E2F3.
ExpressionAtlasiO00716. baseline and differential.
GenevestigatoriO00716.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR015633. E2F.
IPR003316. E2F_TDP.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR12081. PTHR12081. 1 hit.
PfamiPF02319. E2F_TDP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The retinoblastoma protein binds to a family of E2F transcription factors."
    Lees J.A., Saito M., Vidal M., Valentine M., Look T., Harlow E., Dyson N., Helin K.
    Mol. Cell. Biol. 13:7813-7825(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Pre-B cell.
  2. NIEHS SNPs program
    Submitted (SEP-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS ARG-344 AND ASN-389.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Rectum tumor.
  4. "The DNA sequence and analysis of human chromosome 6."
    Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D.
    , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S.
    Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "Prediction of the coding sequences of unidentified human genes. II. The coding sequences of 40 new genes (KIAA0041-KIAA0080) deduced by analysis of cDNA clones from human cell line KG-1."
    Nomura N., Nagase T., Miyajima N., Sazuka T., Tanaka A., Sato S., Seki N., Kawarabayasi Y., Ishikawa K., Tabata S.
    DNA Res. 1:223-229(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 292-465 (ISOFORM 1/2).
    Tissue: Bone marrow.
  6. "EAPP, a novel E2F binding protein that modulates E2F-dependent transcription."
    Novy M., Pohn R., Andorfer P., Novy-Weiland T., Galos B., Schwarzmayr L., Rotheneder H.
    Mol. Biol. Cell 16:2181-2190(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH EAPP.

Entry informationi

Entry nameiE2F3_HUMAN
AccessioniPrimary (citable) accession number: O00716
Secondary accession number(s): Q15000, Q68DT0, Q9BZ44
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 1, 1997
Last modified: February 4, 2015
This is version 143 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.