O00712 (NFIB_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nuclear factor 1 B-type
Short name=NF1-B
Short name=Nuclear factor 1/B

Alternative name(s):
CCAAT-box-binding transcription factor
Short name=CTF
Nuclear factor I/B
Short name=NF-I/B
Short name=NFI-B
TGGCA-binding protein

Gene names

Name:

NFIB

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	420 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.
Subunit structure	Binds DNA as a homodimer.
Subcellular location	Nucleus.
Sequence similarities	Belongs to the CTF/NF-I family. Contains 1 CTF/NF-I DNA-binding domain.

Ontologies

Keywords
Biological process	DNA replication Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Ligand	DNA-binding
Molecular function	Activator
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	Clara cell differentiation Inferred from sequence or structural similarity. Source: UniProtKB DNA replication Inferred from electronic annotation. Source: UniProtKB-KW Type I pneumocyte differentiation Inferred from sequence or structural similarity. Source: UniProtKB Type II pneumocyte differentiation Inferred from sequence or structural similarity. Source: UniProtKB anterior commissure morphogenesis Inferred from sequence or structural similarity. Source: UniProtKB chondrocyte differentiation Inferred from sequence or structural similarity. Source: UniProtKB commissural neuron axon guidance Inferred from sequence or structural similarity. Source: UniProtKB glial cell differentiation Inferred from sequence or structural similarity. Source: UniProtKB lung ciliated cell differentiation Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of DNA binding Inferred from direct assay Ref.2. Source: UniProtKB negative regulation of epithelial cell proliferation involved in lung morphogenesis Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of mesenchymal cell proliferation involved in lung development Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of transcription from RNA polymerase II promoter Inferred from direct assay Ref.2. Source: UniProtKB principal sensory nucleus of trigeminal nerve development Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	cerebellar mossy fiber Inferred from sequence or structural similarity. Source: UniProtKB nucleolus Inferred from direct assay. Source: HPA nucleus Inferred from direct assay Ref.2. Source: UniProtKB
Molecular function	DNA binding Inferred from direct assay Ref.2. Source: UniProtKB RNA polymerase II transcription corepressor activity Inferred from direct assay Ref.2. Source: UniProtKB sequence-specific DNA binding RNA polymerase II transcription factor activity Inferred from direct assay Ref.2. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Note: Additional isoforms seem to exist.

Isoform 1 (identifier: O00712-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O00712-3)

The sequence of this isoform is not available.

Isoform 3 (identifier: O00712-2)

Also known as: NFI-B3;

The sequence of this isoform differs from the canonical sequence as follows:
     187-188: QD → AR
     189-420: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 420	420	Nuclear factor 1 B-type		PRO_0000100195
Regions
	DNA binding	1 – 195	195	CTF/NF-I
Amino acid modifications
	Modified residue	63	1	Phosphoserine By similarity
	Modified residue	264	1	Phosphoserine Ref.6
	Modified residue	265	1	Phosphoserine Ref.6
	Modified residue	292	1	Phosphoserine By similarity
	Modified residue	295	1	Phosphoserine By similarity
	Modified residue	328	1	Phosphoserine Ref.6
	Modified residue	332	1	Phosphoserine Ref.6
Natural variations
	Alternative sequence	187 – 188	2	QD → AR in isoform 3.		VSP_003545
	Alternative sequence	189 – 420	232	Missing in isoform 3.		VSP_003546
Experimental info
	Sequence conflict	17	1	I → M in AAB41899. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 15, 2004. Version 2. Checksum: 0F71635FE9D64FA4	FASTA	420	47,442
	10 20 30 40 50 60 MMYSPICLTQ DEFHPFIEAL LPHVRAIAYT WFNLQARKRK YFKKHEKRMS KDEERAVKDE 70 80 90 100 110 120 LLSEKPEIKQ KWASRLLAKL RKDIRQEYRE DFVLTVTGKK HPCCVLSNPD QKGKIRRIDC 130 140 150 160 170 180 LRQADKVWRL DLVMVILFKG IPLESTDGER LMKSPHCTNP ALCVQPHHIT VSVKELDLFL 190 200 210 220 230 240 AYYVQEQDSG QSGSPSHNDP AKNPPGYLED SFVKSGVFNV SELVRVSRTP ITQGTGVNFP 250 260 270 280 290 300 IGEIPSQPYY HDMNSGVNLQ RSLSSPPSSK RPKTISIDEN MEPSPTGDFY PSPSSPAAGS 310 320 330 340 350 360 RTWHERDQDM SSPTTMKKPE KPLFSSASPQ DSSPRLSTFP QHHHPGIPGV AHSVISTRTP 370 380 390 400 410 420 PPPSPLPFPT QAILPPAPSS YFSHPTIRYP PHLNPQDTLK NYVPSYDPSS PQTSQSWYLG
	Isoform 2 (Sequence not available).		–	–
	Isoform 3 (NFI-B3) [UniParc]. Checksum: C718698597C13336	FASTA	188	22,251
	10 20 30 40 50 60 MMYSPICLTQ DEFHPFIEAL LPHVRAIAYT WFNLQARKRK YFKKHEKRMS KDEERAVKDE 70 80 90 100 110 120 LLSEKPEIKQ KWASRLLAKL RKDIRQEYRE DFVLTVTGKK HPCCVLSNPD QKGKIRRIDC 130 140 150 160 170 180 LRQADKVWRL DLVMVILFKG IPLESTDGER LMKSPHCTNP ALCVQPHHIT VSVKELDLFL AYYVQEAR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of NFIB as recurrent translocation partner gene of HMGIC in pleomorphic adenomas." Geurts J.M.W., Schoenmakers E.F.P.M., Roijer E., Astroem A.-K., Stenman G., van de Ven W.J.M. Oncogene 16:865-872(1998) [PubMed: 9484777] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"NFI-B3, a novel transcriptional repressor of the nuclear factor I family, is generated by alternative RNA processing." Liu Y., Bernard H.U., Apt D. J. Biol. Chem. 272:10739-10745(1997) [PubMed: 9099724] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3). Tissue: Foreskin.
[3]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Cervix.
[5]	"Chromosomal localization of the four genes (NFIA, B, C, and X) for the human transcription factor nuclear factor I by FISH." Qian F., Kruse U., Lichter P., Sippel A.E. Genomics 28:66-73(1995) [PubMed: 7590749] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 20-283.
[6]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-264; SER-265; SER-328 AND SER-332, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U85193 mRNA. Translation: AAB41899.1. U70862 mRNA. Translation: AAB51197.1. BT007266 mRNA. Translation: AAP35930.1. BC001283 mRNA. Translation: AAH01283.1. U07810 mRNA. Translation: AAA93125.1.
IPI	IPI00473116. IPI00953386.
RefSeq	NP_005587.2. NM_005596.3.
UniGene	Hs.644095.
3D structure databases
ProteinModelPortal	O00712.
ModBase	Search...
Protein-protein interaction databases
STRING	O00712.
PTM databases
PhosphoSite	O00712.
Proteomic databases
PRIDE	O00712.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000380959; ENSP00000370346; ENSG00000147862.
GeneID	4781.
KEGG	hsa:4781.
UCSC	uc003zle.1. human.
Organism-specific databases
CTD	4781.
GeneCards	GC09M014077.
H-InvDB	HIX0007921.
HGNC	HGNC:7785. NFIB.
HPA	HPA003956.
MIM	600728. gene.
neXtProt	NX_O00712.
PharmGKB	PA31591.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG06235.
HOVERGEN	HBG006561.
Enzyme and pathway databases
Pathway_Interaction_DB	hnf3apathway. FOXA1 transcription factor network.
Gene expression databases
ArrayExpress	O00712.
Bgee	O00712.
CleanEx	HS_NFIB.
Genevestigator	O00712.
GermOnline	ENSG00000147862. Homo sapiens.
Family and domain databases
InterPro	IPR000647. CTF/NFI. IPR020604. CTF/NFI_DNA-bd-dom. IPR019739. CTF/NFI_DNA-bd_CS. IPR019548. CTF/NFI_DNA-bd_N. IPR003619. MAD_homology1_Dwarfin-type. [Graphical view]
KO	K09169.
PANTHER	PTHR11492. CTF_NFI. 1 hit.
Pfam	PF00859. CTF_NFI. 1 hit. PF03165. MH1. 1 hit. PF10524. NfI_DNAbd_pre-N. 1 hit. [Graphical view]
SMART	SM00523. DWA. 1 hit. [Graphical view]
PROSITE	PS00349. CTF_NFI_1. 1 hit. PS51080. CTF_NFI_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	18436.
SOURCE	Search...

Entry information

Entry name

NFIB_HUMAN

Accession

Primary (citable) accession number: O00712
Secondary accession number(s): O00166, Q12858, Q96J45

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 2001
Last sequence update:	March 15, 2004
Last modified:	November 16, 2011
This is version 105 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.