Reviewed,
UniProtKB/Swiss-Prot O00712 (NFIB_HUMAN)
Last modified
June 16, 2009.
Version 83.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Nuclear factor 1 B-type Short name=Nuclear factor 1/B Short name=NF1-B Alternative name(s): NFI-B NF-I/B CCAAT-box-binding transcription factor Short name=CTF TGGCA-binding protein | ||
| Gene names |
| ||
| Organism | Homo sapiens (Human) | ||
| Taxonomic identifier | 9606 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 420 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication. |
| Subunit structure | Binds DNA as a homodimer. |
| Subcellular location | |
| Sequence similarities | Belongs to the CTF/NF-I family. Contains 1 CTF/NF-I DNA-binding domain. |
Ontologies
| Keywords | |
|---|---|
| Biological process | DNA replication Transcription Transcription regulation |
| Cellular component | Nucleus |
| Coding sequence diversity | Alternative splicing |
| Ligand | DNA-binding |
| Molecular function | Activator |
| PTM | Phosphoprotein |
| Gene Ontology (GO) | |
| Biological process | DNA replication Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription, DNA-dependentInferred from electronic annotation. Source: UniProtKB-KW transcriptionInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | nucleolus Inferred from direct assay. Source: HPA |
| Molecular function | transcription factor activity Ref.5 Traceable author statement. Source: ProtInc |
| Complete GO annotation... | |
Alternative products
| This entry describes 3 isoforms produced by alternative splicing. [Align] [Select] Note: Additional isoforms seem to exist. | ||||||
| Isoform 1 (identifier: O00712-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform 2 (identifier: O00712-3) The sequence of this isoform is not available. | ||||||
| Isoform 3 (identifier: O00712-2) Also known as: NFI-B3; The sequence of this isoform differs from the canonical sequence as follows: 187-188: QD → AR 189-420: Missing. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 420 | 420 | Nuclear factor 1 B-type | PRO_0000100195 | |||||
Regions | |||||||||
| DNA binding | 1 – 195 | 195 | CTF/NF-I | ||||||
Amino acid modifications | |||||||||
| Modified residue | 63 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 264 | 1 | Phosphoserine Ref.6 | ||||||
| Modified residue | 265 | 1 | Phosphoserine Ref.6 | ||||||
| Modified residue | 292 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 295 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 328 | 1 | Phosphoserine Ref.6 | ||||||
| Modified residue | 332 | 1 | Phosphoserine Ref.6 | ||||||
Natural variations | |||||||||
| Alternative sequence | 187 – 188 | 2 | QD → AR in isoform 3. | VSP_003545 | |||||
| Alternative sequence | 189 – 420 | 232 | Missing in isoform 3. | VSP_003546 | |||||
Experimental info | |||||||||
| Sequence conflict | 17 | 1 | I → M in AAB41899. Ref.1 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Identification of NFIB as recurrent translocation partner gene of HMGIC in pleomorphic adenomas." Geurts J.M.W., Schoenmakers E.F.P.M., Roijer E., Astroem A.-K., Stenman G., van de Ven W.J.M. Oncogene 16:865-872(1998) [PubMed: 9484777] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). |
| [2] | "NFI-B3, a novel transcriptional repressor of the nuclear factor I family, is generated by alternative RNA processing." Liu Y., Bernard H.U., Apt D. J. Biol. Chem. 272:10739-10745(1997) [PubMed: 9099724] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3). Tissue: Foreskin. |
| [3] | "Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). |
| [4] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Cervix. |
| [5] | "Chromosomal localization of the four genes (NFIA, B, C, and X) for the human transcription factor nuclear factor I by FISH." Qian F., Kruse U., Lichter P., Sippel A.E. Genomics 28:66-73(1995) [PubMed: 7590749] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 20-283. |
| [6] | "A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-264; SER-265; SER-328 AND SER-332, MASS SPECTROMETRY. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| U85193 mRNA. Translation: AAB41899.1. U70862 mRNA. Translation: AAB51197.1. BT007266 mRNA. Translation: AAP35930.1. BC001283 mRNA. Translation: AAH01283.1. U07810 mRNA. Translation: AAA93125.1. | |
| IPI | IPI00473116. IPI00872141. |
| RefSeq | NP_005587.2. |
| UniGene | Hs.644095 |
3D structure databases | |
| ModBase | Search... |
PTM databases | |
| PhosphoSite | O00712. |
Proteomic databases | |
| PRIDE | O00712. |
Genome annotation databases | |
| Ensembl | ENSG00000147862. Homo sapiens. [Contig view] |
| GeneID | 4781. |
| KEGG | hsa:4781. |
Organism-specific databases | |
| GeneCards | GC09M014077. |
| H-InvDB | HIX0007921. |
| HGNC | HGNC:7785. NFIB. |
| HPA | HPA003956. |
| MIM | 600728. gene. |
| PharmGKB | PA31591. |
| GenAtlas | Search... |
Phylogenomic databases | |
| HOVERGEN | O00712. |
Enzyme and pathway databases | |
| Pathway_Interaction_DB | hnf3apathway. FOXA1 transcription factor network. |
Gene expression databases | |
| ArrayExpress | O00712. |
| Bgee | O00712. |
| CleanEx | HS_NFIB. |
| GermOnline | ENSG00000147862. Homo sapiens. |
Family and domain databases | |
| InterPro | IPR000647. CTF/NFI_DNA-bd. IPR019739. CTF/NFI_DNA-bd_CS. IPR019548. CTF/NFI_DNA-bd_N. IPR003619. MAD_homology1_Dwarfin-type. [Graphical view] |
| PANTHER | PTHR11492. CTF_NFI. 1 hit. |
| Pfam | PF00859. CTF_NFI. 1 hit. PF03165. MH1. 1 hit. PF10524. NfI_DNAbd_pre-N. 1 hit. [Graphical view] |
| SMART | SM00523. DWA. 1 hit. [Graphical view] |
| PROSITE | PS00349. CTF_NFI_1. 1 hit. PS51080. CTF_NFI_2. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 18436. |
| SOURCE | Search... |
Entry information
| Entry name | NFIB_HUMAN | ||||||||
| Accession | Primary (citable) accession number: O00712 Secondary accession number(s): O00166, Q12858, Q96J45 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| Human chromosome 9 Human chromosome 9: entries, gene names and cross-references to MIM |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |

Clusters with


