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Protein

Nuclear factor 1 B-type

Gene

NFIB

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi1 – 195CTF/NF-IPROSITE-ProRule annotationAdd BLAST195

GO - Molecular functioni

  • DNA binding Source: UniProtKB
  • RNA polymerase II proximal promoter sequence-specific DNA binding Source: NTNU_SB
  • RNA polymerase II transcription corepressor activity Source: UniProtKB
  • RNA polymerase II transcription factor activity, sequence-specific DNA binding Source: UniProtKB
  • transcriptional activator activity, RNA polymerase II proximal promoter sequence-specific DNA binding Source: NTNU_SB

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding
Biological processDNA replication, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-HSA-73980 RNA Polymerase III Transcription Termination
R-HSA-749476 RNA Polymerase III Abortive And Retractive Initiation
SignaLinkiO00712
SIGNORiO00712

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear factor 1 B-type
Short name:
NF1-B
Short name:
Nuclear factor 1/B
Alternative name(s):
CCAAT-box-binding transcription factor
Short name:
CTF
Nuclear factor I/B
Short name:
NF-I/B
Short name:
NFI-B
TGGCA-binding protein
Gene namesi
Name:NFIB
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 9

Organism-specific databases

EuPathDBiHostDB:ENSG00000147862.14
HGNCiHGNC:7785 NFIB
MIMi600728 gene
neXtProtiNX_O00712

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi4781
OpenTargetsiENSG00000147862
PharmGKBiPA31591

Polymorphism and mutation databases

BioMutaiNFIB

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001001951 – 420Nuclear factor 1 B-typeAdd BLAST420

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei264PhosphoserineCombined sources1
Modified residuei286PhosphothreonineBy similarity1
Modified residuei292PhosphoserineCombined sources1
Modified residuei295PhosphoserineCombined sources1
Modified residuei312PhosphoserineCombined sources1
Modified residuei328PhosphoserineCombined sources1
Modified residuei333PhosphoserineCombined sources1
Modified residuei335Asymmetric dimethylarginineBy similarity1
Modified residuei388Asymmetric dimethylarginineBy similarity1

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

EPDiO00712
PaxDbiO00712
PeptideAtlasiO00712
PRIDEiO00712
ProteomicsDBi48006
48007 [O00712-2]

PTM databases

iPTMnetiO00712
PhosphoSitePlusiO00712

Expressioni

Gene expression databases

BgeeiENSG00000147862
CleanExiHS_NFIB
ExpressionAtlasiO00712 baseline and differential
GenevisibleiO00712 HS

Organism-specific databases

HPAiHPA003956

Interactioni

Subunit structurei

Binds DNA as a homodimer.

Protein-protein interaction databases

BioGridi110853, 14 interactors
DIPiDIP-61433N
IntActiO00712, 6 interactors
STRINGi9606.ENSP00000370340

Structurei

3D structure databases

ProteinModelPortaliO00712
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the CTF/NF-I family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3663 Eukaryota
ENOG410Y16G LUCA
GeneTreeiENSGT00390000009905
HOGENOMiHOG000013028
HOVERGENiHBG006561
InParanoidiO00712
KOiK09169
PhylomeDBiO00712
TreeFamiTF313889

Family and domain databases

InterProiView protein in InterPro
IPR000647 CTF/NFI
IPR020604 CTF/NFI_DNA-bd-dom
IPR019739 CTF/NFI_DNA-bd_CS
IPR019548 CTF/NFI_DNA-bd_N
IPR003619 MAD_homology1_Dwarfin-type
PANTHERiPTHR11492 PTHR11492, 1 hit
PfamiView protein in Pfam
PF00859 CTF_NFI, 1 hit
PF03165 MH1, 1 hit
PF10524 NfI_DNAbd_pre-N, 1 hit
SMARTiView protein in SMART
SM00523 DWA, 1 hit
PROSITEiView protein in PROSITE
PS00349 CTF_NFI_1, 1 hit
PS51080 CTF_NFI_2, 1 hit

Sequences (6)i

Sequence statusi: Complete.

This entry describes 6 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms seem to exist.
Isoform 1 (identifier: O00712-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MMYSPICLTQ DEFHPFIEAL LPHVRAIAYT WFNLQARKRK YFKKHEKRMS
60 70 80 90 100
KDEERAVKDE LLSEKPEIKQ KWASRLLAKL RKDIRQEYRE DFVLTVTGKK
110 120 130 140 150
HPCCVLSNPD QKGKIRRIDC LRQADKVWRL DLVMVILFKG IPLESTDGER
160 170 180 190 200
LMKSPHCTNP ALCVQPHHIT VSVKELDLFL AYYVQEQDSG QSGSPSHNDP
210 220 230 240 250
AKNPPGYLED SFVKSGVFNV SELVRVSRTP ITQGTGVNFP IGEIPSQPYY
260 270 280 290 300
HDMNSGVNLQ RSLSSPPSSK RPKTISIDEN MEPSPTGDFY PSPSSPAAGS
310 320 330 340 350
RTWHERDQDM SSPTTMKKPE KPLFSSASPQ DSSPRLSTFP QHHHPGIPGV
360 370 380 390 400
AHSVISTRTP PPPSPLPFPT QAILPPAPSS YFSHPTIRYP PHLNPQDTLK
410 420
NYVPSYDPSS PQTSQSWYLG
Length:420
Mass (Da):47,442
Last modified:March 15, 2004 - v2
Checksum:i0F71635FE9D64FA4
GO
Isoform 2 (identifier: O00712-3)
Sequence is not available
Length:
Mass (Da):
Isoform 3 (identifier: O00712-2) [UniParc]FASTAAdd to basket
Also known as: NFI-B3

The sequence of this isoform differs from the canonical sequence as follows:
     187-188: QD → AR
     189-420: Missing.

Show »
Length:188
Mass (Da):22,251
Checksum:iC718698597C13336
GO
Isoform 4 (identifier: O00712-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-10: MMYSPICLTQ → MERIPVSVDFWVVCCAVLKCNPGIPMERIPVSVDFWVVCCAVLKCNPGIPKRMSTLCFGFS

Note: No experimental confirmation available.
Show »
Length:471
Mass (Da):53,049
Checksum:iD2797FFB1527C066
GO
Isoform 5 (identifier: O00712-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     416-416: S → PNGSGQVVGK...DPSFLHQQQS

Note: No experimental confirmation available.
Show »
Length:494
Mass (Da):55,181
Checksum:i55BE3377EEEA2C07
GO
Isoform 6 (identifier: O00712-6) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-252: Missing.
     416-420: SWYLG → PNGSGQVVGK...EPFLQAETSN

Note: No experimental confirmation available.
Show »
Length:309
Mass (Da):33,525
Checksum:i8DA70FF7F8A8CEEF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti17I → M in AAB41899 (PubMed:9484777).Curated1
Sequence conflicti37R → G in BAD18416 (PubMed:14702039).Curated1
Sequence conflicti74S → F in BX648845 (PubMed:17974005).Curated1
Sequence conflicti198N → S in BAD18416 (PubMed:14702039).Curated1
Sequence conflicti284S → R in BX648845 (PubMed:17974005).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0547131 – 252Missing in isoform 6. 1 PublicationAdd BLAST252
Alternative sequenceiVSP_0444621 – 10MMYSPICLTQ → MERIPVSVDFWVVCCAVLKC NPGIPMERIPVSVDFWVVCC AVLKCNPGIPKRMSTLCFGF S in isoform 4. 1 Publication10
Alternative sequenceiVSP_003545187 – 188QD → AR in isoform 3. 1 Publication2
Alternative sequenceiVSP_003546189 – 420Missing in isoform 3. 1 PublicationAdd BLAST232
Alternative sequenceiVSP_054714416 – 420SWYLG → PNGSGQVVGKVPGHFTPVLA PSPHPSAVRPVTLSMTDTKP ITTSTEAYTASGTSQANRYV GLSPRDPSFLHQQQLRICDW TMNQNGRHLYPSTSEDTLGI TWQSPGTWASLVPFQVSNRT PILPANVQNYGLNIIGEPFL QAETSN in isoform 6. 1 Publication5
Alternative sequenceiVSP_045065416S → PNGSGQVVGKVPGHFTPVLA PSPHPSAVRPVTLSMTDTKP ITTSTEAYTASGTSQANRYV GLSPRDPSFLHQQQS in isoform 5. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U85193 mRNA Translation: AAB41899.1
U70862 mRNA Translation: AAB51197.1
BT007266 mRNA Translation: AAP35930.1
AK131233 mRNA Translation: BAD18416.1
BX648416 mRNA Translation: CAH56156.1
BX648845 mRNA No translation available.
AL136366 Genomic DNA No translation available.
AL441963 Genomic DNA No translation available.
AL137017 Genomic DNA No translation available.
AL449443 Genomic DNA No translation available.
CH471071 Genomic DNA Translation: EAW58697.1
CH471071 Genomic DNA Translation: EAW58699.1
BC001283 mRNA Translation: AAH01283.1
U07810 mRNA Translation: AAA93125.1
CCDSiCCDS55291.1 [O00712-5]
CCDS6474.1 [O00712-1]
CCDS65007.1 [O00712-6]
RefSeqiNP_001177666.1, NM_001190737.1 [O00712-5]
NP_001177667.1, NM_001190738.1
NP_001269716.1, NM_001282787.1 [O00712-6]
NP_005587.2, NM_005596.3 [O00712-1]
UniGeneiHs.644095

Genome annotation databases

EnsembliENST00000380953; ENSP00000370340; ENSG00000147862 [O00712-5]
ENST00000380959; ENSP00000370346; ENSG00000147862 [O00712-1]
ENST00000543693; ENSP00000442888; ENSG00000147862 [O00712-6]
GeneIDi4781
KEGGihsa:4781
UCSCiuc003zld.4 human [O00712-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiNFIB_HUMAN
AccessioniPrimary (citable) accession number: O00712
Secondary accession number(s): G3V1P1
, H7BYE8, O00166, Q12858, Q5VW29, Q63HM5, Q6ZNF9, Q96J45
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: March 15, 2004
Last modified: June 20, 2018
This is version 164 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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