Reviewed, UniProtKB/Swiss-Prot O00625 (PIR_HUMAN)

Names and origin

Protein names

Recommended name:
    Pirin

Gene names

Name:

PIR

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	290 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Subunit structure	Interacts with NF1/CTF1.
Subcellular location	Nucleus. Note: Predominantly localized in dot-like subnuclear structures.
Tissue specificity	Expressed in all human tissues with highest level in heart and liver.
Sequence similarities	Belongs to the pirin family.

Ontologies

Keywords
Cellular component	Nucleus
Coding sequence diversity	Polymorphism
Ligand	Iron Metal-binding
Technical term	3D-structure
Gene Ontology (GO)
Biological process	transcription from RNA polymerase II promoter Ref.1 Traceable author statement. Source: ProtInc
Cellular component	nucleus Ref.1 Traceable author statement. Source: ProtInc
Molecular function	iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW transcription cofactor activity Ref.1 Traceable author statement. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 290

290

Pirin

PRO_0000214051

Sites

Metal binding

56

1

Iron

Metal binding

58

1

Iron

Metal binding

101

1

Iron

Metal binding

103

1

Iron

Natural variations

Natural variant

228

1

V → A: dbSNP rs34104000.

VAR_050543

Secondary structure

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290

Helix Strand Turn

Details...

Beta strand

7 – 12

6

Beta strand

15 – 18

4

Beta strand

22 – 26

5

Beta strand

39 – 47

9

Beta strand

52 – 67

16

Beta strand

69 – 71

3

Beta strand

73 – 77

5

Beta strand

82 – 85

4

Beta strand

90 – 94

5

Beta strand

99 – 105

7

Beta strand

107 – 109

3

Beta strand

111 – 119

9

Helix

122 – 124

3

Beta strand

130 – 134

5

Helix

136 – 138

3

Beta strand

147 – 156

10

Beta strand

169 – 176

8

Beta strand

181 – 185

5

Beta strand

191 – 199

9

Beta strand

201 – 204

4

Beta strand

210 – 212

3

Beta strand

216 – 220

5

Beta strand

224 – 229

6

Beta strand

232 – 234

3

Beta strand

236 – 243

8

Beta strand

251 – 253

3

Beta strand

256 – 260

5

Helix

261 – 272

12

Helix

279 – 281

3

Sequences

	Sequence		Length	Mass (Da)
	O00625-1 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: D06AC100F356496D	FASTA	290	32,113
	10 20 30 40 50 60 MGSSKKVTLS VLSREQSEGV GARVRRSIGR PELKNLDPFL LFDEFKGGRP GGFPDHPHRG 70 80 90 100 110 120 FETVSYLLEG GSMAHEDFCG HTGKMNPGDL QWMTAGRGIL HAEMPCSEEP AHGLQLWVNL 130 140 150 160 170 180 RSSEKMVEPQ YQELKSEEIP KPSKDGVTVA VISGEALGIK SKVYTRTPTL YLDFKLDPGA 190 200 210 220 230 240 KHSQPIPKGW TSFIYTISGD VYIGPDDAQQ KIEPHHTAVL GEGDSVQVEN KDPKRSHFVL 250 260 270 280 290 IAGEPLREPV IQHGPFVMNT NEEISQAILD FRNAKNGFER AKTWKSKIGN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of pirin, a novel highly conserved nuclear protein." Wendler W.M.F., Kremmer E., Foerster R., Winnacker E.-L. J. Biol. Chem. 272:8482-8489(1997) [PubMed: 9079676] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Cervix carcinoma.
[2]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Placenta.
[4]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[5]	"Crystal structure of human pirin: an iron-binding nuclear protein and transcription cofactor." Pang H., Bartlam M., Zeng Q., Miyatake H., Hisano T., Miki K., Wong L.L., Gao G.F., Rao Z. J. Biol. Chem. 279:1491-1498(2004) [PubMed: 14573596] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

Y07868 mRNA. Translation: CAA69195.1.
Y07867 mRNA. Translation: CAA69194.1.
BT019583 mRNA. Translation: AAV38390.1.
BT019584 mRNA. Translation: AAV38391.1.
BC002517 mRNA. Translation: AAH02517.1.

IPI

IPI00012575.

RefSeq

NP_001018119.1.
NP_003653.1.

UniGene

Hs.495728

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1J1L	X-ray	2.10	A	1-290	[»]

ModBase

Search...

Proteomic databases

PeptideAtlas

O00625.

PRIDE

O00625.

Genome annotation databases

Ensembl

ENSG00000087842. Homo sapiens. [Contig view]

GeneID

8544.

KEGG

hsa:8544.

Organism-specific databases

GeneCards

GC0XM015312.

H-InvDB

HIX0019995.

HGNC

HGNC:30048. PIR.

HPA

HPA000697.

MIM

603329. gene.

PharmGKB

PA134870022.

GenAtlas

Search...

Phylogenomic databases

HOGENOM

O00625.

HOVERGEN

O00625.

OMA

O00625. KDPKRSH.

Gene expression databases

ArrayExpress

O00625.

Bgee

O00625.

CleanEx

HS_PIR.

GermOnline

ENSG00000087842. Homo sapiens.

Family and domain databases

InterPro

IPR012093. Pirin.
IPR008778. Pirin_C.
IPR003829. Pirin_N.
[Graphical view]

PANTHER

PTHR13903. Pirin. 1 hit.

Pfam

PF02678. Pirin. 1 hit.
PF05726. Pirin_C. 1 hit.
[Graphical view]

PIRSF

PIRSF006232. Pirin. 1 hit.

ProtoNet

Search...

Other Resources

NextBio

32006.

SOURCE

Search...

Entry information

Entry name

PIR_HUMAN

Accession

Primary (citable) accession number: O00625
Secondary accession number(s): Q5U0G0

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 2001
Last sequence update:	July 1, 1997
Last modified:	June 16, 2009
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)