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O00625

- PIR_HUMAN

UniProt

O00625 - PIR_HUMAN

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Protein

Pirin

Gene

PIR

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Transcriptional coregulator of NF-kappa-B which facilitates binding of NF-kappa-B proteins to target kappa-B genes in a redox-state-dependent manner. May be required for efficient terminal myeloid maturation of hematopoietic cells. Has quercetin 2,3-dioxygenase activity (in vitro).4 Publications

Catalytic activityi

Quercetin + O2 = 2-(3,4-dihydroxybenzoyloxy)-4,6-dihydroxybenzoate + CO + H+.1 Publication

Cofactori

Fe cation2 PublicationsNote: Binds 1 Fe cation per subunit.2 Publications

Enzyme regulationi

Inhibited by kojic acid, sodium diethyldithiocarbamate and 1,10-phenanthroline monohydrochloride.1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi56 – 561Iron
Metal bindingi58 – 581Iron
Metal bindingi101 – 1011Iron
Metal bindingi103 – 1031Iron

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB
  2. quercetin 2,3-dioxygenase activity Source: UniProtKB
  3. transcription cofactor activity Source: UniProtKB

GO - Biological processi

  1. monocyte differentiation Source: MGI
  2. regulation of transcription, DNA-templated Source: UniProtKB-KW
  3. transcription from RNA polymerase II promoter Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00724.

Names & Taxonomyi

Protein namesi
Recommended name:
Pirin (EC:1.13.11.24)
Alternative name(s):
Probable quercetin 2,3-dioxygenase PIR
Short name:
Probable quercetinase
Gene namesi
Name:PIR
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome X

Organism-specific databases

HGNCiHGNC:30048. PIR.

Subcellular locationi

Nucleus. Cytoplasm
Note: Predominantly localized in dot-like subnuclear structures. Cytoplasmic localization of PIR seems to positively correlate with melanoma progression.

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134870022.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 290290PirinPRO_0000214051Add
BLAST

Proteomic databases

MaxQBiO00625.
PaxDbiO00625.
PeptideAtlasiO00625.
PRIDEiO00625.

2D gel databases

UCD-2DPAGEO00625.

PTM databases

PhosphoSiteiO00625.

Expressioni

Tissue specificityi

Highly expressed in a subset of melanomas. Detected at very low levels in most tissues (at protein level). Expressed in all tissues, with highest level of expression in heart and liver.2 Publications

Inductioni

Up-regulated in CD34+ cells upon myelomonocytic differentiation. Down-regulated in many acute myeloid leukemias. Up-regulated in primary bronchial epithelial cells exposed to cigarette smoke extract.2 Publications

Gene expression databases

BgeeiO00625.
CleanExiHS_PIR.
GenevestigatoriO00625.

Organism-specific databases

HPAiHPA000697.

Interactioni

Subunit structurei

May interact with NF1/CTF1. Interacts with BCL3. Identified in a complex comprised of PIR, BLC3, NFKB1 and target DNA.4 Publications

Protein-protein interaction databases

BioGridi114114. 15 interactions.
IntActiO00625. 1 interaction.
MINTiMINT-1189585.
STRINGi9606.ENSP00000369785.

Structurei

Secondary structure

1
290
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi7 – 126Combined sources
Beta strandi14 – 185Combined sources
Beta strandi22 – 265Combined sources
Helixi31 – 333Combined sources
Beta strandi39 – 479Combined sources
Beta strandi52 – 6716Combined sources
Beta strandi69 – 713Combined sources
Beta strandi73 – 775Combined sources
Beta strandi82 – 854Combined sources
Beta strandi90 – 945Combined sources
Beta strandi99 – 1057Combined sources
Beta strandi107 – 1093Combined sources
Beta strandi111 – 1199Combined sources
Helixi122 – 1243Combined sources
Beta strandi130 – 1345Combined sources
Helixi136 – 1383Combined sources
Beta strandi147 – 15610Combined sources
Beta strandi169 – 1768Combined sources
Beta strandi181 – 1855Combined sources
Beta strandi191 – 1999Combined sources
Beta strandi201 – 2044Combined sources
Beta strandi210 – 2123Combined sources
Beta strandi216 – 2205Combined sources
Beta strandi224 – 2296Combined sources
Beta strandi232 – 2343Combined sources
Beta strandi236 – 2438Combined sources
Beta strandi251 – 2533Combined sources
Beta strandi256 – 2605Combined sources
Helixi261 – 27212Combined sources
Helixi279 – 2813Combined sources
Turni287 – 2893Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1J1LX-ray2.10A1-290[»]
3ACLX-ray2.35A2-290[»]
4EROX-ray2.65A1-290[»]
4EWAX-ray2.47A1-290[»]
4EWDX-ray2.15A1-290[»]
4EWEX-ray1.56A1-290[»]
4GULX-ray1.80A3-290[»]
4HLTX-ray1.70A3-290[»]
ProteinModelPortaliO00625.
SMRiO00625. Positions 3-290.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO00625.

Family & Domainsi

Sequence similaritiesi

Belongs to the pirin family.Curated

Phylogenomic databases

eggNOGiCOG1741.
GeneTreeiENSGT00390000008044.
HOGENOMiHOG000248360.
HOVERGENiHBG019151.
InParanoidiO00625.
KOiK06911.
OMAiQWMTAAR.
OrthoDBiEOG7JMGFH.
PhylomeDBiO00625.
TreeFamiTF300002.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
InterProiIPR012093. Pirin.
IPR008778. Pirin_C_dom.
IPR003829. Pirin_N_dom.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PANTHERiPTHR13903. PTHR13903. 1 hit.
PfamiPF02678. Pirin. 1 hit.
PF05726. Pirin_C. 1 hit.
[Graphical view]
PIRSFiPIRSF006232. Pirin. 1 hit.
SUPFAMiSSF51182. SSF51182. 1 hit.

Sequencei

Sequence statusi: Complete.

O00625-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGSSKKVTLS VLSREQSEGV GARVRRSIGR PELKNLDPFL LFDEFKGGRP
60 70 80 90 100
GGFPDHPHRG FETVSYLLEG GSMAHEDFCG HTGKMNPGDL QWMTAGRGIL
110 120 130 140 150
HAEMPCSEEP AHGLQLWVNL RSSEKMVEPQ YQELKSEEIP KPSKDGVTVA
160 170 180 190 200
VISGEALGIK SKVYTRTPTL YLDFKLDPGA KHSQPIPKGW TSFIYTISGD
210 220 230 240 250
VYIGPDDAQQ KIEPHHTAVL GEGDSVQVEN KDPKRSHFVL IAGEPLREPV
260 270 280 290
IQHGPFVMNT NEEISQAILD FRNAKNGFER AKTWKSKIGN
Length:290
Mass (Da):32,113
Last modified:July 1, 1997 - v1
Checksum:iD06AC100F356496D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti24 – 241V → D in CAG46621. 1 PublicationCurated
Sequence conflicti162 – 1621K → R in CAG46621. 1 PublicationCurated

Polymorphismi

Genetic variations in PIR might have a sex-specific influence on bone mineral density differences in some populations, as reported by PubMed:19766747. In a cohort of 4000 Chinese, a significant statistical association has been identified, in women but not in men, between the intronic SNP rs5935970 and lumbar spine bone mineral density, and between a haplotype composed of three SNPs with bone mineral density at other sites.

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti228 – 2281V → A.
Corresponds to variant rs34104000 [ dbSNP | Ensembl ].
VAR_050543

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y07868 mRNA. Translation: CAA69195.1.
Y07867 mRNA. Translation: CAA69194.1.
CR541822 mRNA. Translation: CAG46621.1.
BT019583 mRNA. Translation: AAV38390.1.
BT019584 mRNA. Translation: AAV38391.1.
BC002517 mRNA. Translation: AAH02517.1.
CCDSiCCDS14167.1.
RefSeqiNP_001018119.1. NM_001018109.2.
NP_003653.1. NM_003662.3.
UniGeneiHs.495728.
Hs.732521.

Genome annotation databases

EnsembliENST00000380420; ENSP00000369785; ENSG00000087842.
ENST00000380421; ENSP00000369786; ENSG00000087842.
GeneIDi8544.
KEGGihsa:8544.
UCSCiuc004cwu.3. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y07868 mRNA. Translation: CAA69195.1 .
Y07867 mRNA. Translation: CAA69194.1 .
CR541822 mRNA. Translation: CAG46621.1 .
BT019583 mRNA. Translation: AAV38390.1 .
BT019584 mRNA. Translation: AAV38391.1 .
BC002517 mRNA. Translation: AAH02517.1 .
CCDSi CCDS14167.1.
RefSeqi NP_001018119.1. NM_001018109.2.
NP_003653.1. NM_003662.3.
UniGenei Hs.495728.
Hs.732521.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1J1L X-ray 2.10 A 1-290 [» ]
3ACL X-ray 2.35 A 2-290 [» ]
4ERO X-ray 2.65 A 1-290 [» ]
4EWA X-ray 2.47 A 1-290 [» ]
4EWD X-ray 2.15 A 1-290 [» ]
4EWE X-ray 1.56 A 1-290 [» ]
4GUL X-ray 1.80 A 3-290 [» ]
4HLT X-ray 1.70 A 3-290 [» ]
ProteinModelPortali O00625.
SMRi O00625. Positions 3-290.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 114114. 15 interactions.
IntActi O00625. 1 interaction.
MINTi MINT-1189585.
STRINGi 9606.ENSP00000369785.

Chemistry

BindingDBi O00625.
ChEMBLi CHEMBL2010627.

PTM databases

PhosphoSitei O00625.

2D gel databases

UCD-2DPAGE O00625.

Proteomic databases

MaxQBi O00625.
PaxDbi O00625.
PeptideAtlasi O00625.
PRIDEi O00625.

Protocols and materials databases

DNASUi 8544.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000380420 ; ENSP00000369785 ; ENSG00000087842 .
ENST00000380421 ; ENSP00000369786 ; ENSG00000087842 .
GeneIDi 8544.
KEGGi hsa:8544.
UCSCi uc004cwu.3. human.

Organism-specific databases

CTDi 8544.
GeneCardsi GC0XM015402.
HGNCi HGNC:30048. PIR.
HPAi HPA000697.
MIMi 300931. gene.
neXtProti NX_O00625.
PharmGKBi PA134870022.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG1741.
GeneTreei ENSGT00390000008044.
HOGENOMi HOG000248360.
HOVERGENi HBG019151.
InParanoidi O00625.
KOi K06911.
OMAi QWMTAAR.
OrthoDBi EOG7JMGFH.
PhylomeDBi O00625.
TreeFami TF300002.

Enzyme and pathway databases

UniPathwayi UPA00724 .

Miscellaneous databases

EvolutionaryTracei O00625.
GeneWikii PIR_(gene).
GenomeRNAii 8544.
NextBioi 32006.
PROi O00625.
SOURCEi Search...

Gene expression databases

Bgeei O00625.
CleanExi HS_PIR.
Genevestigatori O00625.

Family and domain databases

Gene3Di 2.60.120.10. 1 hit.
InterProi IPR012093. Pirin.
IPR008778. Pirin_C_dom.
IPR003829. Pirin_N_dom.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view ]
PANTHERi PTHR13903. PTHR13903. 1 hit.
Pfami PF02678. Pirin. 1 hit.
PF05726. Pirin_C. 1 hit.
[Graphical view ]
PIRSFi PIRSF006232. Pirin. 1 hit.
SUPFAMi SSF51182. SSF51182. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of pirin, a novel highly conserved nuclear protein."
    Wendler W.M.F., Kremmer E., Foerster R., Winnacker E.-L.
    J. Biol. Chem. 272:8482-8489(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROPOSED FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Tissue: Cervix carcinoma.
  2. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
    Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  3. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
    Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
    Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Placenta.
  5. "The Bcl-3 oncoprotein acts as a bridging factor between NF-kappaB/Rel and nuclear co-regulators."
    Dechend R., Hirano F., Lehmann K., Heissmeyer V., Ansieau S., Wulczyn F.G., Scheidereit C., Leutz A.
    Oncogene 18:3316-3323(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH BCL3, IDENTIFICATION IN A COMPLEX WITH BLC3; NFKB1; PIR AND TARGET DNA.
  6. "Structural and biochemical analysis reveal pirins to possess quercetinase activity."
    Adams M., Jia Z.
    J. Biol. Chem. 280:28675-28682(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: CATALYTIC ACTIVITY, ENZYME REGULATION.
  7. "Upregulation of pirin expression by chronic cigarette smoking is associated with bronchial epithelial cell apoptosis."
    Gelbman B.D., Heguy A., O'Connor T.P., Zabner J., Crystal R.G.
    Respir. Res. 8:10-10(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION.
  8. "Pirin delocalization in melanoma progression identified by high content immuno-detection based approaches."
    Licciulli S., Luise C., Zanardi A., Giorgetti L., Viale G., Lanfrancone L., Carbone R., Alcalay M.
    BMC Cell Biol. 11:5-5(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  9. "Sex-specific effect of Pirin gene on bone mineral density in a cohort of 4000 Chinese."
    Tang N.L., Liao C.D., Ching J.K., Suen E.W., Chan I.H., Orwoll E., Ho S.C., Chan F.W., Kwok A.W., Kwok T., Woo J., Leung P.C.
    Bone 46:543-550(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: POSSIBLE INFLUENCE OF PIR POLYMORPHISMS ON BONE MINERAL DENSITY.
  10. "Pirin downregulation is a feature of AML and leads to impairment of terminal myeloid differentiation."
    Licciulli S., Cambiaghi V., Scafetta G., Gruszka A.M., Alcalay M.
    Leukemia 24:429-437(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION.
  11. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  12. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  13. "Crystal structure of human pirin: an iron-binding nuclear protein and transcription cofactor."
    Pang H., Bartlam M., Zeng Q., Miyatake H., Hisano T., Miki K., Wong L.L., Gao G.F., Rao Z.
    J. Biol. Chem. 279:1491-1498(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) IN COMPLEX WITH IRON IONS, COFACTOR.
  14. "A small-molecule inhibitor shows that pirin regulates migration of melanoma cells."
    Miyazaki I., Simizu S., Okumura H., Takagi S., Osada H.
    Nat. Chem. Biol. 6:667-673(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.35 ANGSTROMS) IN COMPLEX WITH A SYNTHETIC INHIBITOR AND IRON IONS, INTERACTION WITH BCL3, COFACTOR, FUNCTION.
  15. Cited for: X-RAY CRYSTALLOGRAPHY (1.56 ANGSTROMS), FUNCTION.

Entry informationi

Entry nameiPIR_HUMAN
AccessioniPrimary (citable) accession number: O00625
Secondary accession number(s): Q5U0G0, Q6FHD2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: July 1, 1997
Last modified: November 26, 2014
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome X
    Human chromosome X: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  7. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3