ID   GBRP_HUMAN              Reviewed;         440 AA.
AC   O00591; A8KA36; D3DQL2; Q32MJ1;
DT   15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1997, sequence version 1.
DT   27-MAR-2024, entry version 186.
DE   RecName: Full=Gamma-aminobutyric acid receptor subunit pi;
DE   AltName: Full=GABA(A) receptor subunit pi;
DE   Flags: Precursor;
GN   Name=GABRP;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=9182563; DOI=10.1074/jbc.272.24.15346;
RA   Hedblom E., Kirkness E.F.;
RT   "A novel class of GABAA receptor subunit in tissues of the reproductive
RT   system.";
RL   J. Biol. Chem. 272:15346-15350(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Johnson E.K., Hedblom E., Kirkness E.F.;
RT   "Structure and localization of the gene encoding the human GABA-A receptor
RT   pi subunit (GABRP).";
RL   Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Trachea;
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA   Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA   Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA   Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA   Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA   Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA   Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA   Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA   Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA   Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA   Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA   Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA   Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA   Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA   Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA   Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA   Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA   Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA   Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA   Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA   Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA   Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA   Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA   Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA   Hunkapiller M.W., Myers E.W., Venter J.C.;
RL   Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT LEU-391.
RC   TISSUE=Lung;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [6]
RP   VARIANT [LARGE SCALE ANALYSIS] ARG-416.
RX   PubMed=16959974; DOI=10.1126/science.1133427;
RA   Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D.,
RA   Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P.,
RA   Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V.,
RA   Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H.,
RA   Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W.,
RA   Velculescu V.E.;
RT   "The consensus coding sequences of human breast and colorectal cancers.";
RL   Science 314:268-274(2006).
CC   -!- FUNCTION: GABA, the major inhibitory neurotransmitter in the vertebrate
CC       brain, mediates neuronal inhibition by binding to the
CC       GABA/benzodiazepine receptor and opening an integral chloride channel.
CC       In the uterus, the function of the receptor appears to be related to
CC       tissue contractility. The binding of this pI subunit with other GABA(A)
CC       receptor subunits alters the sensitivity of recombinant receptors to
CC       modulatory agents such as pregnanolone.
CC   -!- SUBUNIT: Generally pentameric. There are five types of GABA(A) receptor
CC       chains: alpha, beta, gamma, delta, and epsilon. A sixth class of
CC       subunit: Rho form homomeric GABA receptors that do not appear to
CC       coexist with GABA(A) receptor subunits but with GABA(C) receptor
CC       subunits. Subunit Pi can also bind this complex.
CC   -!- SUBCELLULAR LOCATION: Postsynaptic cell membrane; Multi-pass membrane
CC       protein. Cell membrane; Multi-pass membrane protein.
CC   -!- TISSUE SPECIFICITY: Most abundant in the uterus, also expressed in
CC       lung, thymus and prostate.
CC   -!- SIMILARITY: Belongs to the ligand-gated ion channel (TC 1.A.9) family.
CC       Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRP sub-
CC       subfamily. {ECO:0000305}.
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DR   EMBL; U95367; AAC51357.1; -; mRNA.
DR   EMBL; AF009702; AAC24194.1; -; Genomic_DNA.
DR   EMBL; AF009694; AAC24194.1; JOINED; Genomic_DNA.
DR   EMBL; AF009695; AAC24194.1; JOINED; Genomic_DNA.
DR   EMBL; AF009696; AAC24194.1; JOINED; Genomic_DNA.
DR   EMBL; AF009697; AAC24194.1; JOINED; Genomic_DNA.
DR   EMBL; AF009698; AAC24194.1; JOINED; Genomic_DNA.
DR   EMBL; AF009699; AAC24194.1; JOINED; Genomic_DNA.
DR   EMBL; AF009700; AAC24194.1; JOINED; Genomic_DNA.
DR   EMBL; AF009701; AAC24194.1; JOINED; Genomic_DNA.
DR   EMBL; AK292901; BAF85590.1; -; mRNA.
DR   EMBL; CH471062; EAW61467.1; -; Genomic_DNA.
DR   EMBL; CH471062; EAW61468.1; -; Genomic_DNA.
DR   EMBL; BC074810; AAH74810.1; -; mRNA.
DR   EMBL; BC074865; AAH74865.1; -; mRNA.
DR   EMBL; BC109105; AAI09106.1; -; mRNA.
DR   EMBL; BC109106; AAI09107.1; -; mRNA.
DR   CCDS; CCDS4375.1; -.
DR   RefSeq; NP_001278914.1; NM_001291985.1.
DR   RefSeq; NP_055026.1; NM_014211.2.
DR   AlphaFoldDB; O00591; -.
DR   SMR; O00591; -.
DR   BioGRID; 108842; 1.
DR   STRING; 9606.ENSP00000430100; -.
DR   ChEMBL; CHEMBL2093872; -.
DR   DrugBank; DB12537; 1,2-Benzodiazepine.
DR   DrugBank; DB00546; Adinazolam.
DR   DrugBank; DB00404; Alprazolam.
DR   DrugBank; DB00543; Amoxapine.
DR   DrugBank; DB11901; Apalutamide.
DR   DrugBank; DB14719; Bentazepam.
DR   DrugBank; DB11859; Brexanolone.
DR   DrugBank; DB01558; Bromazepam.
DR   DrugBank; DB09017; Brotizolam.
DR   DrugBank; DB00237; Butabarbital.
DR   DrugBank; DB00241; Butalbital.
DR   DrugBank; DB01489; Camazepam.
DR   DrugBank; DB00475; Chlordiazepoxide.
DR   DrugBank; DB14715; Cinazepam.
DR   DrugBank; DB01594; Cinolazepam.
DR   DrugBank; DB00349; Clobazam.
DR   DrugBank; DB01068; Clonazepam.
DR   DrugBank; DB00628; Clorazepic acid.
DR   DrugBank; DB01559; Clotiazepam.
DR   DrugBank; DB01553; Cloxazolam.
DR   DrugBank; DB01511; Delorazepam.
DR   DrugBank; DB01189; Desflurane.
DR   DrugBank; DB00829; Diazepam.
DR   DrugBank; DB13837; Doxefazepam.
DR   DrugBank; DB00228; Enflurane.
DR   DrugBank; DB01215; Estazolam.
DR   DrugBank; DB00402; Eszopiclone.
DR   DrugBank; DB00898; Ethanol.
DR   DrugBank; DB00189; Ethchlorvynol.
DR   DrugBank; DB01545; Ethyl loflazepate.
DR   DrugBank; DB09166; Etizolam.
DR   DrugBank; DB00292; Etomidate.
DR   DrugBank; DB01567; Fludiazepam.
DR   DrugBank; DB01205; Flumazenil.
DR   DrugBank; DB01544; Flunitrazepam.
DR   DrugBank; DB00690; Flurazepam.
DR   DrugBank; DB05087; Ganaxolone.
DR   DrugBank; DB01437; Glutethimide.
DR   DrugBank; DB00801; Halazepam.
DR   DrugBank; DB01159; Halothane.
DR   DrugBank; DB00753; Isoflurane.
DR   DrugBank; DB01587; Ketazolam.
DR   DrugBank; DB00555; Lamotrigine.
DR   DrugBank; DB13643; Loprazolam.
DR   DrugBank; DB00186; Lorazepam.
DR   DrugBank; DB13872; Lormetazepam.
DR   DrugBank; DB13437; Medazepam.
DR   DrugBank; DB00603; Medroxyprogesterone acetate.
DR   DrugBank; DB01043; Memantine.
DR   DrugBank; DB00371; Meprobamate.
DR   DrugBank; DB00463; Metharbital.
DR   DrugBank; DB01028; Methoxyflurane.
DR   DrugBank; DB01107; Methyprylon.
DR   DrugBank; DB15489; Mexazolam.
DR   DrugBank; DB00683; Midazolam.
DR   DrugBank; DB01595; Nitrazepam.
DR   DrugBank; DB14028; Nordazepam.
DR   DrugBank; DB00842; Oxazepam.
DR   DrugBank; DB14672; Oxazepam acetate.
DR   DrugBank; DB00312; Pentobarbital.
DR   DrugBank; DB00252; Phenytoin.
DR   DrugBank; DB13335; Pinazepam.
DR   DrugBank; DB01708; Prasterone.
DR   DrugBank; DB01588; Prazepam.
DR   DrugBank; DB00794; Primidone.
DR   DrugBank; DB00818; Propofol.
DR   DrugBank; DB01589; Quazepam.
DR   DrugBank; DB12404; Remimazolam.
DR   DrugBank; DB01236; Sevoflurane.
DR   DrugBank; DB09118; Stiripentol.
DR   DrugBank; DB00306; Talbutal.
DR   DrugBank; DB01956; Taurine.
DR   DrugBank; DB00231; Temazepam.
DR   DrugBank; DB11582; Thiocolchicoside.
DR   DrugBank; DB00897; Triazolam.
DR   DrugCentral; O00591; -.
DR   GlyCosmos; O00591; 5 sites, No reported glycans.
DR   GlyGen; O00591; 5 sites.
DR   iPTMnet; O00591; -.
DR   PhosphoSitePlus; O00591; -.
DR   BioMuta; GABRP; -.
DR   jPOST; O00591; -.
DR   MassIVE; O00591; -.
DR   MaxQB; O00591; -.
DR   PaxDb; 9606-ENSP00000430100; -.
DR   PeptideAtlas; O00591; -.
DR   ABCD; O00591; 2 sequenced antibodies.
DR   Antibodypedia; 4442; 108 antibodies from 20 providers.
DR   DNASU; 2568; -.
DR   Ensembl; ENST00000265294.9; ENSP00000265294.4; ENSG00000094755.17.
DR   Ensembl; ENST00000518525.5; ENSP00000430100.1; ENSG00000094755.17.
DR   GeneID; 2568; -.
DR   KEGG; hsa:2568; -.
DR   MANE-Select; ENST00000265294.9; ENSP00000265294.4; NM_014211.3; NP_055026.1.
DR   UCSC; uc003mau.4; human.
DR   AGR; HGNC:4089; -.
DR   CTD; 2568; -.
DR   DisGeNET; 2568; -.
DR   GeneCards; GABRP; -.
DR   HGNC; HGNC:4089; GABRP.
DR   HPA; ENSG00000094755; Tissue enhanced (breast, esophagus).
DR   MIM; 602729; gene.
DR   neXtProt; NX_O00591; -.
DR   OpenTargets; ENSG00000094755; -.
DR   PharmGKB; PA28503; -.
DR   VEuPathDB; HostDB:ENSG00000094755; -.
DR   eggNOG; KOG3643; Eukaryota.
DR   GeneTree; ENSGT00940000160813; -.
DR   HOGENOM; CLU_010920_0_1_1; -.
DR   InParanoid; O00591; -.
DR   OMA; TTVTCNM; -.
DR   OrthoDB; 4265336at2759; -.
DR   PhylomeDB; O00591; -.
DR   TreeFam; TF315453; -.
DR   PathwayCommons; O00591; -.
DR   BioGRID-ORCS; 2568; 13 hits in 1154 CRISPR screens.
DR   ChiTaRS; GABRP; human.
DR   GeneWiki; GABRP; -.
DR   GenomeRNAi; 2568; -.
DR   Pharos; O00591; Tclin.
DR   PRO; PR:O00591; -.
DR   Proteomes; UP000005640; Chromosome 5.
DR   RNAct; O00591; Protein.
DR   Bgee; ENSG00000094755; Expressed in nasal cavity epithelium and 120 other cell types or tissues.
DR   ExpressionAtlas; O00591; baseline and differential.
DR   GO; GO:0034707; C:chloride channel complex; IEA:UniProtKB-KW.
DR   GO; GO:1902711; C:GABA-A receptor complex; IBA:GO_Central.
DR   GO; GO:0043005; C:neuron projection; IBA:GO_Central.
DR   GO; GO:0045211; C:postsynaptic membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0045202; C:synapse; IBA:GO_Central.
DR   GO; GO:0005254; F:chloride channel activity; IEA:UniProtKB-KW.
DR   GO; GO:0005230; F:extracellular ligand-gated monoatomic ion channel activity; IEA:InterPro.
DR   GO; GO:0004890; F:GABA-A receptor activity; TAS:ProtInc.
DR   GO; GO:0030594; F:neurotransmitter receptor activity; IBA:GO_Central.
DR   GO; GO:1902476; P:chloride transmembrane transport; IBA:GO_Central.
DR   CDD; cd19058; LGIC_TM_GABAAR_pi; 1.
DR   Gene3D; 2.70.170.10; Neurotransmitter-gated ion-channel ligand-binding domain; 1.
DR   Gene3D; 1.20.58.390; Neurotransmitter-gated ion-channel transmembrane domain; 1.
DR   InterPro; IPR006028; GABAA/Glycine_rcpt.
DR   InterPro; IPR008100; GABAAp_rcpt.
DR   InterPro; IPR047032; GABAAR_pi_TM.
DR   InterPro; IPR006202; Neur_chan_lig-bd.
DR   InterPro; IPR036734; Neur_chan_lig-bd_sf.
DR   InterPro; IPR006201; Neur_channel.
DR   InterPro; IPR036719; Neuro-gated_channel_TM_sf.
DR   InterPro; IPR038050; Neuro_actylchol_rec.
DR   InterPro; IPR006029; Neurotrans-gated_channel_TM.
DR   InterPro; IPR018000; Neurotransmitter_ion_chnl_CS.
DR   NCBIfam; TIGR00860; LIC; 1.
DR   PANTHER; PTHR18945:SF33; GAMMA-AMINOBUTYRIC ACID RECEPTOR SUBUNIT PI; 1.
DR   PANTHER; PTHR18945; NEUROTRANSMITTER GATED ION CHANNEL; 1.
DR   Pfam; PF02931; Neur_chan_LBD; 1.
DR   Pfam; PF02932; Neur_chan_memb; 1.
DR   PRINTS; PR00253; GABAARECEPTR.
DR   PRINTS; PR01724; GABAARPI.
DR   PRINTS; PR00252; NRIONCHANNEL.
DR   SUPFAM; SSF90112; Neurotransmitter-gated ion-channel transmembrane pore; 1.
DR   SUPFAM; SSF63712; Nicotinic receptor ligand binding domain-like; 1.
DR   PROSITE; PS00236; NEUROTR_ION_CHANNEL; 1.
DR   Genevisible; O00591; HS.
PE   2: Evidence at transcript level;
KW   Cell membrane; Chloride; Chloride channel; Disulfide bond; Glycoprotein;
KW   Ion channel; Ion transport; Membrane; Postsynaptic cell membrane;
KW   Reference proteome; Signal; Synapse; Transmembrane; Transmembrane helix;
KW   Transport.
FT   SIGNAL          1..16
FT                   /evidence="ECO:0000255"
FT   CHAIN           17..440
FT                   /note="Gamma-aminobutyric acid receptor subunit pi"
FT                   /id="PRO_0000000492"
FT   TOPO_DOM        17..242
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        243..266
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        270..292
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        305..327
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        328..416
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        417..438
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        43
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        102
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        145
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        196
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        228
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        160..174
FT                   /evidence="ECO:0000250"
FT   VARIANT         391
FT                   /note="F -> L (in dbSNP:rs1063310)"
FT                   /evidence="ECO:0000269|PubMed:15489334"
FT                   /id="VAR_020323"
FT   VARIANT         416
FT                   /note="H -> R (in a breast cancer sample; somatic mutation;
FT                   dbSNP:rs1290926314)"
FT                   /evidence="ECO:0000269|PubMed:16959974"
FT                   /id="VAR_036034"
SQ   SEQUENCE   440 AA;  50640 MW;  92CA66C264560718 CRC64;
     MNYSLHLAFV CLSLFTERMC IQGSQFNVEV GRSDKLSLPG FENLTAGYNK FLRPNFGGEP
     VQIALTLDIA SISSISESNM DYTATIYLRQ RWMDQRLVFE GNKSFTLDAR LVEFLWVPDT
     YIVESKKSFL HEVTVGNRLI RLFSNGTVLY ALRITTTVAC NMDLSKYPMD TQTCKLQLES
     WGYDGNDVEF TWLRGNDSVR GLEHLRLAQY TIERYFTLVT RSQQETGNYT RLVLQFELRR
     NVLYFILETY VPSTFLVVLS WVSFWISLDS VPARTCIGVT TVLSMTTLMI GSRTSLPNTN
     CFIKAIDVYL GICFSFVFGA LLEYAVAHYS SLQQMAAKDR GTTKEVEEVS ITNIINSSIS
     SFKRKISFAS IEISSDNVDY SDLTMKTSDK FKFVFREKMG RIVDYFTIQN PSNVDHYSKL
     LFPLIFMLAN VFYWAYYMYF
//