Reviewed, UniProtKB/Swiss-Prot O00585 (CCL21_HUMAN)

Names and origin

Protein names

Recommended name:
    C-C motif chemokine 21
Alternative name(s):
    Small-inducible cytokine A21
    Beta chemokine exodus-2
    6Ckine
    Secondary lymphoid-tissue chemokine
      Short name=SLC

Gene names

Name:	CCL21
Synonyms:	SCYA21
ORF Names:	UNQ784/PRO1600

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	134 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Inhibits hemopoiesis and stimulates chemotaxis. Chemotactic in vitro for thymocytes and activated T-cells, but not for B-cells, macrophages, or neutrophils. Shows preferential activity towards naive T-cells. May play a role in mediating homing of lymphocytes to secondary lymphoid organs.
Subunit structure	Binds to CCR7. Ref.8
Subcellular location	Secreted.
Tissue specificity	Highly expressed in high endothelial venules of lymph nodes, spleen and appendix. Intermediate levels found in small intestine, thyroid gland and trachea. Low level expression in thymus, bone marrow, liver, and pancreas. Also found in tonsil, fetal heart and fetal spleen.
Sequence similarities	Belongs to the intercrine beta (chemokine CC) family.

Ontologies

Keywords
Biological process	Chemotaxis Inflammatory response
Cellular component	Secreted
Domain	Signal
Molecular function	Cytokine
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	cell-cell signaling Ref.1 Traceable author statement. Source: ProtInc chemotaxis Traceable author statement. Source: ProtInc immune response Traceable author statement. Source: ProtInc inflammatory response Ref.1 Traceable author statement. Source: ProtInc signal transduction Non-traceable author statement. Source: ProtInc
Cellular component	extracellular space Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	chemokine activity Traceable author statement. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Ref.3
	Chain	24 – 134	111	C-C motif chemokine 21		PRO_0000005220
Regions
	Region	98 – 134	37	C-terminal basic extension
Amino acid modifications
	Disulfide bond	31 ↔ 57		By similarity
	Disulfide bond	32 ↔ 75		By similarity
	Disulfide bond	103 ↔ 122		Potential

Sequences

	Sequence		Length	Mass (Da)
	O00585-1 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: FB48C2F71CB4A9D2	FASTA	134	14,646
	10 20 30 40 50 60 MAQSLALSLL ILVLAFGIPR TQGSDGGAQD CCLKYSQRKI PAKVVRSYRK QEPSLGCSIP 70 80 90 100 110 120 AILFLPRKRS QAELCADPKE LWVQQLMQHL DKTPSPQKPA QGCRKDRGAS KTGKKGKGSK 130 GCKRTERSQT PKGP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of a novel human CC chemokine secondary lymphoid-tissue chemokine that is a potent chemoattractant for lymphocytes and mapped to chromosome 9p13." Nagira M., Imai T., Hieshima K., Kusuda J., Ridanpaeae M., Takagi S., Nishimura M., Kakizaki M., Nomiyama H., Yoshie O. J. Biol. Chem. 272:19518-19524(1997) [PubMed: 9235955] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Identification and characterization of a novel beta chemokine containing six conserved cysteines." Hedrick J.A., Zlotnik A. J. Immunol. 159:1589-1593(1997) [PubMed: 9257816] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Isolation and characterization of Exodus-2, a novel C-C chemokine with a unique 37-amino acid carboxyl-terminal extension." Hromas R., Kim C.H., Klemsz M., Krathwohl M., Fife K., Cooper S., Schnizlein-Bick C., Broxmeyer H.E. J. Immunol. 159:2554-2558(1997) [PubMed: 9300671] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 24-33.
[4]	"A chemokine expressed in lymphoid high endothelial venules promotes the adhesion and chemotaxis of naive T lymphocytes." Gunn M.D., Tangemann K., Tam C., Cyster J.G., Rosen S.D., Williams L.T. Proc. Natl. Acad. Sci. U.S.A. 95:258-263(1998) [PubMed: 9419363] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION.
[5]	"Genomic organisation of human SLC." Reiterer P., Bernhardt G., Lipp M. Submitted (APR-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[6]	"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M. Genome Res. 13:2265-2270(2003) [PubMed: 12975309] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Pancreas and Spleen.
[8]	"Secondary lymphoid-tissue chemokine is a functional ligand for the CC chemokine receptor CCR7." Yoshida R., Nagira M., Kitaura M., Imagawa N., Imai T., Yoshie O. J. Biol. Chem. 273:7118-7122(1998) [PubMed: 9507024] [Abstract] Cited for: RECEPTOR INTERACTION.
+	Additional computationally mapped references.

Web resources

Wikipedia

CCL21 entry

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB002409 mRNA. Translation: BAA21817.1. AF001979 mRNA. Translation: AAB86594.1. U88320 mRNA. Translation: AAB91454.1. AJ005654 Genomic DNA. Translation: CAA06653.1. AY358887 mRNA. Translation: AAQ89246.1. BC027918 mRNA. Translation: AAH27918.1.
IPI	IPI00012174.
RefSeq	NP_002980.1.
UniGene	Hs.57907
3D structure databases
HSSP	HSSP built from PDB template 1F2L based on UniProtKB P78423.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:5854N.
IntAct	O00585. 1 interaction.
PTM databases
PhosphoSite	O00585.
Proteomic databases
PRIDE	O00585.
Genome annotation databases
Ensembl	ENSG00000137077. Homo sapiens. [Contig view]
GeneID	6366.
KEGG	hsa:6366.
Organism-specific databases
GeneCards	GC09M034699.
H-InvDB	HIX0008004.
HGNC	HGNC:10620. CCL21.
HPA	CAB005067.
MIM	602737. gene.
PharmGKB	PA35552.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	O00585.
HOVERGEN	O00585.
OMA	O00585. CPISAIL.
Enzyme and pathway databases
Reactome	REACT_14797. Signaling by GPCR.
Gene expression databases
ArrayExpress	O00585.
Bgee	O00585.
CleanEx	HS_CCL21.
GermOnline	ENSG00000137077. Homo sapiens.
Family and domain databases
InterPro	IPR000827. CC_chemkine_sml_CS. IPR001811. Chemokine_IL8. [Graphical view]
Pfam	PF00048. IL8. 1 hit. [Graphical view]
SMART	SM00199. SCY. 1 hit. [Graphical view]
PROSITE	PS00472. SMALL_CYTOKINES_CC. False negative. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	24734.
PMAP-CutDB	O00585.
SOURCE	Search...

Entry information

Entry name

CCL21_HUMAN

Accession

Primary (citable) accession number: O00585

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	July 1, 1997
Last modified:	June 16, 2009
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)