EBAG9

Functionⁱ

May participate in suppression of cell proliferation and induces apoptotic cell death through activation of interleukin-1-beta converting enzyme (ICE)-like proteases.3 Publications

Miscellaneous

May serve as a prognostic marker for cancers such as adenocarcinomas of the lung and breast cancers. It is present and overexpressed in many patients suffering from breast carcinomas, its level of expression correlates with tumor grade, suggesting that it may be involved in cancer immune escape. According to PubMed:12672804, it is however not directly a tumor-associated antigen, but it rather modulates surface expression of tumor-associated O-linked glycan Tn when it is overexpressed, suggesting that it contributes indirectly to the antigenicity of tumor cells.

GO - Molecular functionⁱ

peptidase activator activity involved in apoptotic process
Source: UniProtKB
Non-traceable author statementⁱ
- 10426319

Complete GO annotation...

GO - Biological processⁱ

regulation of cell growth
Source: UniProtKB
Non-traceable author statementⁱ
- 10426319

Complete GO annotation...

Keywordsⁱ

Biological process	Apoptosis

Biological process

Apoptosis

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Receptor-binding cancer antigen expressed on SiSo cells Alternative name(s): Cancer-associated surface antigen RCAS1 Estrogen receptor-binding fragment-associated gene 9 protein
Gene namesⁱ	Name:EBAG9 Synonyms:RCAS1
Organismⁱ	Homo sapiens (Human)
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Chromosome 8

Organism-specific databases

Human Gene Nomenclature Database More...HGNCⁱ	HGNC:3123. EBAG9.

HGNCⁱ

HGNC:3123. EBAG9.

Subcellular locationⁱ

Golgi apparatus membrane
1 Publication
Manual assertion based on experiment inⁱ
- Ref.10
  "The Golgi protein RCAS1 controls cell surface expression of tumor-associated O-linked glycan antigens."
  Engelsberg A., Hermosilla R., Karsten U., Schuelein R., Doerken B., Rehm A.
  J. Biol. Chem. 278:22998-23007(2003) [PubMed] [Europe PMC] [Abstract]
  Cited for: FUNCTION, SUBCELLULAR LOCATION.
; Single-pass type III membrane protein
1 Publication
Manual assertion based on experiment inⁱ
- Ref.10
  "The Golgi protein RCAS1 controls cell surface expression of tumor-associated O-linked glycan antigens."
  Engelsberg A., Hermosilla R., Karsten U., Schuelein R., Doerken B., Rehm A.
  J. Biol. Chem. 278:22998-23007(2003) [PubMed] [Europe PMC] [Abstract]
  Cited for: FUNCTION, SUBCELLULAR LOCATION.

Note:

According to PubMed:10426319, it also exists as a soluble form which has the same biological activities. The existence of such soluble form is however uncertain.

Topology

Feature key	Position(s)	DescriptionActions	Length
Topological domainⁱ	1 – 6	ExtracellularSequence analysis	6
Transmembraneⁱ	7 – 27	Helical; Signal-anchor for type III membrane proteinSequence analysisAdd BLAST	21
Topological domainⁱ	28 – 213	CytoplasmicSequence analysisAdd BLAST	186

GO - Cellular componentⁱ

Golgi membrane Source: UniProtKB-SubCell
integral component of membrane Source: UniProtKB-KW
secretory granule Source: Ensembl

Complete GO annotation...

Keywords - Cellular componentⁱ

Golgi apparatus, Membrane

Pathology & Biotechⁱ

Organism-specific databases

DisGeNET More...DisGeNETⁱ	9166.
Open Targets More...OpenTargetsⁱ	ENSG00000147654.
PharmGKBⁱ	PA27581.

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000097195	1 – 213	Receptor-binding cancer antigen expressed on SiSo cellsAdd BLAST		213

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	36	PhosphoserineCombined sources	1
Modified residueⁱ	41	PhosphothreonineCombined sources	1
Modified residueⁱ	94	PhosphotyrosineCombined sources	1

Keywords - PTMⁱ

Phosphoprotein

Proteomic databases

EPDⁱ	O00559.
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	O00559.
PaxDbⁱ	O00559.
PeptideAtlas More...PeptideAtlasⁱ	O00559.
PRIDEⁱ	O00559.

PTM databases

iPTMnetⁱ	O00559.
PhosphoSitePlusⁱ	O00559.
SwissPalmⁱ	O00559.

Expressionⁱ

Tissue specificityⁱ

Widely expressed. Expressed in ovary, testis, prostate, thymus, muscle and heart, but not in small intestine, colon, lymph nodes, or peripherical blood lymphocytes. The protein is not detected in any of the above organs.

Inductionⁱ

By estrogen.

Gene expression databases

Bgeeⁱ	ENSG00000147654.
CleanExⁱ	HS_EBAG9.
ExpressionAtlasⁱ	O00559. baseline and differential.
Genevisibleⁱ	O00559. HS.

Organism-specific databases

Human Protein Atlas More...HPAⁱ	CAB072810. CAB072811. CAB072812. HPA021153. HPA021154.

HPAⁱ

CAB072810.
CAB072811.
CAB072812.
HPA021153.
HPA021154.

Interactionⁱ

Subunit structureⁱ

Homodimer.

Protein-protein interaction databases

BioGridⁱ	114607. 3 interactors.
IntActⁱ	O00559. 3 interactors.
STRINGⁱ	9606.ENSP00000337675.

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	O00559.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Coiled coil

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Coiled coilⁱ	163 – 211	Sequence analysisAdd BLAST		49

Compositional bias

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Compositional biasⁱ	160 – 163	Poly-Glu		4

Domainⁱ

The coiled coil domain is necessary for the homodimerization.

Keywords - Domainⁱ

Coiled coil, Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGⁱ	ENOG410IE57. Eukaryota. ENOG41101KS. LUCA.
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00390000004040.
HOGENOMⁱ	HOG000073537.
HOVERGENⁱ	HBG059707.
InParanoidⁱ	O00559.
OMAⁱ	FRLFKIC.
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0L0F.
PhylomeDBⁱ	O00559.
TreeFamⁱ	TF326584.

Family and domain databases

InterProⁱ	View protein in InterPro IPR017025. Cancer-assoc_antigen_RCAS1.
PANTHERⁱ	PTHR15208. PTHR15208. 1 hit.
PIRSFⁱ	PIRSF034247. RCAS1. 1 hit.

Sequences (2)ⁱ

Sequence statusⁱ: Complete.

This entry describes 2 isoformsⁱ produced by alternative splicing. Align Add to basket Added to basket

Isoform 1 (identifier: O00559-1) [UniParc]FASTA Add to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAITQFRLFK FCTCLATVFS FLKRLICRSG RGRKLSGDQI TLPTTVDYSS 
        60         70         80         90        100
VPKQTDVEEW TSWDEDAPTS VKIEGGNGNV ATQQNSLEQL EPDYFKDMTP 
       110        120        130        140        150
TIRKTQKIVI KKREPLNFGI PDGSTGFSSR LAATQDLPFI HQSSELGDLD 
       160        170        180        190        200
TWQENTNAWE EEEDAAWQAE EVLRQQKLAD REKRAAEQQR KKMEKEAQRL 
       210 
MKKEQNKIGV KLS

Length:213

Mass (Da):24,377

Last modified:July 1, 1997 - v1

Checksum:ⁱB115E741E23891C5

GO

Isoform 2 (identifier: O00559-2) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
174-174: R → RSRTNVCLLCSLLFHHPTPTSTPYINQSVKIERVSLGQWSYGKSKE

« Hide

        10         20         30         40         50
MAITQFRLFK FCTCLATVFS FLKRLICRSG RGRKLSGDQI TLPTTVDYSS 
        60         70         80         90        100
VPKQTDVEEW TSWDEDAPTS VKIEGGNGNV ATQQNSLEQL EPDYFKDMTP 
       110        120        130        140        150
TIRKTQKIVI KKREPLNFGI PDGSTGFSSR LAATQDLPFI HQSSELGDLD 
       160        170        180        190        200
TWQENTNAWE EEEDAAWQAE EVLRSRTNVC LLCSLLFHHP TPTSTPYINQ 
       210        220        230        240        250
SVKIERVSLG QWSYGKSKEQ QKLADREKRA AEQQRKKMEK EAQRLMKKEQ 

NKIGVKLS

Show »

Length:258

Mass (Da):29,466

Checksum:ⁱ98ECCBCEA9BA1B9C

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	183	K → E in AAH05249 (PubMed:15489334).Curated		1
Sequence conflictⁱ	199	R → Q in BAF83340 (PubMed:14702039).Curated		1

Alternative sequence

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Alternative sequenceⁱVSP_055503	174	R → RSRTNVCLLCSLLFHHPTPT STPYINQSVKIERVSLGQWS YGKSKE in isoform 2. 1 Publication		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF006265 mRNA. Translation: AAB61617.1. AB007619 mRNA. Translation: BAA22572.1. AY653072 mRNA. Translation: AAU85838.1. AK290651 mRNA. Translation: BAF83340.1. CR456984 mRNA. Translation: CAG33265.1. AC079061 Genomic DNA. No translation available. AP000427 Genomic DNA. No translation available. BC005249 mRNA. Translation: AAH05249.1. BC017729 mRNA. Translation: AAH17729.1. BC022506 mRNA. Translation: AAH22506.1.
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS6313.1. [O00559-1]
NCBI Reference Sequences More...RefSeqⁱ	NP_001265867.1. NM_001278938.1. [O00559-1] NP_004206.1. NM_004215.4. [O00559-1] NP_936056.1. NM_198120.2. [O00559-1] XP_016869449.1. XM_017013960.1. [O00559-1]
UniGeneⁱ	Hs.409368. Hs.632960.

Genome annotation databases

Ensemblⁱ	ENST00000337573; ENSP00000337675; ENSG00000147654. [O00559-1] ENST00000395785; ENSP00000379131; ENSG00000147654. [O00559-1] ENST00000531677; ENSP00000432082; ENSG00000147654. [O00559-2] ENST00000614147; ENSP00000477734; ENSG00000147654. [O00559-2] ENST00000620557; ENSP00000477645; ENSG00000147654. [O00559-1]
GeneIDⁱ	9166.
KEGGⁱ	hsa:9166.
UCSC genome browser More...UCSCⁱ	uc003ynf.5. human. [O00559-1]

Keywords - Coding sequence diversityⁱ

Alternative splicing

Cross-referencesⁱ

Web resourcesⁱ

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF006265 mRNA. Translation: AAB61617.1. AB007619 mRNA. Translation: BAA22572.1. AY653072 mRNA. Translation: AAU85838.1. AK290651 mRNA. Translation: BAF83340.1. CR456984 mRNA. Translation: CAG33265.1. AC079061 Genomic DNA. No translation available. AP000427 Genomic DNA. No translation available. BC005249 mRNA. Translation: AAH05249.1. BC017729 mRNA. Translation: AAH17729.1. BC022506 mRNA. Translation: AAH22506.1.
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS6313.1. [O00559-1]
NCBI Reference Sequences More...RefSeqⁱ	NP_001265867.1. NM_001278938.1. [O00559-1] NP_004206.1. NM_004215.4. [O00559-1] NP_936056.1. NM_198120.2. [O00559-1] XP_016869449.1. XM_017013960.1. [O00559-1]
UniGeneⁱ	Hs.409368. Hs.632960.

3D structure databases

ProteinModelPortalⁱ	O00559.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	114607. 3 interactors.
IntActⁱ	O00559. 3 interactors.
STRINGⁱ	9606.ENSP00000337675.

PTM databases

iPTMnetⁱ	O00559.
PhosphoSitePlusⁱ	O00559.
SwissPalmⁱ	O00559.

Proteomic databases

EPDⁱ	O00559.
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	O00559.
PaxDbⁱ	O00559.
PeptideAtlas More...PeptideAtlasⁱ	O00559.
PRIDEⁱ	O00559.

Protocols and materials databases

The DNASU plasmid repository More...DNASUⁱ	9166.
Structural Biology Knowledgebase	Search...

Genome annotation databases

Ensemblⁱ	ENST00000337573; ENSP00000337675; ENSG00000147654. [O00559-1] ENST00000395785; ENSP00000379131; ENSG00000147654. [O00559-1] ENST00000531677; ENSP00000432082; ENSG00000147654. [O00559-2] ENST00000614147; ENSP00000477734; ENSG00000147654. [O00559-2] ENST00000620557; ENSP00000477645; ENSG00000147654. [O00559-1]
GeneIDⁱ	9166.
KEGGⁱ	hsa:9166.
UCSC genome browser More...UCSCⁱ	uc003ynf.5. human. [O00559-1]

Organism-specific databases

CTDⁱ	9166.
DisGeNET More...DisGeNETⁱ	9166.
GeneCardsⁱ	EBAG9.
Human Gene Nomenclature Database More...HGNCⁱ	HGNC:3123. EBAG9.
Human Protein Atlas More...HPAⁱ	CAB072810. CAB072811. CAB072812. HPA021153. HPA021154.
MIMⁱ	605772. gene.
neXtProtⁱ	NX_O00559.
Open Targets More...OpenTargetsⁱ	ENSG00000147654.
PharmGKBⁱ	PA27581.
GenAtlas: human gene database More...GenAtlasⁱ	Search...

Phylogenomic databases

eggNOGⁱ	ENOG410IE57. Eukaryota. ENOG41101KS. LUCA.
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00390000004040.
HOGENOMⁱ	HOG000073537.
HOVERGENⁱ	HBG059707.
InParanoidⁱ	O00559.
OMAⁱ	FRLFKIC.
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0L0F.
PhylomeDBⁱ	O00559.
TreeFamⁱ	TF326584.

Miscellaneous databases

ChiTaRSⁱ	EBAG9. human.
GeneWikiⁱ	EBAG9.
GenomeRNAiⁱ	9166.
Protein Ontology More...PROⁱ	PR:O00559.
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSG00000147654.
CleanExⁱ	HS_EBAG9.
ExpressionAtlasⁱ	O00559. baseline and differential.
Genevisibleⁱ	O00559. HS.

Family and domain databases

InterProⁱ	View protein in InterPro IPR017025. Cancer-assoc_antigen_RCAS1.
PANTHERⁱ	PTHR15208. PTHR15208. 1 hit.
PIRSFⁱ	PIRSF034247. RCAS1. 1 hit.
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	RCAS1_HUMAN
Accessionⁱ	O00559Primary (citable) accession number: O00559 Secondary accession number(s): A8K3N6 , Q5Y8C7, Q6IB20, Q9BS76
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
	Last sequence update:	July 1, 1997
	Last modified:	March 15, 2017
	This is version 145 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Caution

It was initially reported to be a ligand for some putative receptor present on T-, B-, natural killer (NK) cells and various human cell lines. However, PubMed:12672804 showed that it does not bind any receptor.Curated

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

Human chromosome 8
Human chromosome 8: entries, gene names and cross-references to MIM
MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Similar proteinsⁱ

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:

100%	UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%	UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%	UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - O00559 (RCAS1_HUMAN)

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Receptor-binding cancer antigen expressed on SiSo cells

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Miscellaneous

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Topology

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Organism-specific databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Organism-specific databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Coiled coil

Compositional bias

Phylogenomic databases

Family and domain databases

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including length and molecular weight.<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequences (2)i

Experimental Info

Alternative sequence

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

<p>This subsection of the <a href="http://www.uniprot.org/manual/cross_references_section">Cross-references</a> section provides links to various web resources that are relevant for a specific protein.<p><a href='/help/web_resource' target='_top'>More...</a></p>Web resourcesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Caution

Documents

Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequences (2)ⁱ

Cross-referencesⁱ

Web resourcesⁱ

Entry informationⁱ

Miscellaneousⁱ