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Protein

Ladinin-1

Gene

LAD1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Anchoring filament protein which is a component of the basement membrane zone.By similarity

GO - Molecular functioni

  • structural molecule activity Source: ProtInc
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Ladinin-1
Short name:
Lad-1
Alternative name(s):
Linear IgA disease antigen
Short name:
LADA
Gene namesi
Name:LAD1
Synonyms:LAD
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:6472. LAD1.

Subcellular locationi

GO - Cellular componenti

  • basement membrane Source: UniProtKB-SubCell
  • extracellular exosome Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Basement membrane, Extracellular matrix, Secreted

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA30263.

Polymorphism and mutation databases

BioMutaiLAD1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 517517Ladinin-1PRO_0000084349Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei38 – 381Phosphoserine2 Publications
Modified residuei64 – 641Phosphoserine2 Publications
Modified residuei121 – 1211Phosphoserine1 Publication
Modified residuei123 – 1231Phosphoserine1 Publication
Modified residuei356 – 3561Phosphoserine1 Publication
Modified residuei394 – 3941Phosphoserine1 Publication
Modified residuei485 – 4851Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiO00515.
PaxDbiO00515.
PRIDEiO00515.

PTM databases

PhosphoSiteiO00515.

Expressioni

Gene expression databases

BgeeiO00515.
CleanExiHS_LAD1.
ExpressionAtlasiO00515. baseline and differential.
GenevestigatoriO00515.

Organism-specific databases

HPAiHPA007256.
HPA028732.

Interactioni

Protein-protein interaction databases

BioGridi110095. 3 interactions.
IntActiO00515. 3 interactions.
MINTiMINT-3974996.
STRINGi9606.ENSP00000375829.

Structurei

3D structure databases

ProteinModelPortaliO00515.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati203 – 2053SEK 1
Repeati209 – 2113SEK 2
Repeati215 – 2173SEK 3
Repeati221 – 2233SEK 4
Repeati227 – 2293SEK 5
Repeati239 – 2413SEK 6
Repeati257 – 2593SEK 7
Repeati269 – 2713SEK 8

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni203 – 271698 X SEK repeatsAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi28 – 358Poly-Arg

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG84480.
GeneTreeiENSGT00390000005256.
HOGENOMiHOG000113274.
HOVERGENiHBG006240.
InParanoidiO00515.
PhylomeDBiO00515.
TreeFamiTF335896.

Family and domain databases

InterProiIPR017404. Ladinin_1.
[Graphical view]
PIRSFiPIRSF038144. Ladinin_1. 1 hit.

Sequencei

Sequence statusi: Complete.

O00515-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVSRKDWSA LSSLARQRTL EDEEEQERER RRRHRNLSST TDDEAPRLSQ
60 70 80 90 100
NGDRQASASE RLPSVEEAEV PKPLPPASKD EDEDIQSILR TRQERRQRRQ
110 120 130 140 150
VVEAAQAPIQ ERLEAEEGRN SLSPVQATQK PLVSKKELEI PPRRRLSREQ
160 170 180 190 200
RGPWALEEES LVGREPEERK KGVPEKSPVL EKSSMPKKTA PEKSLVSDKT
210 220 230 240 250
SISEKVLASE KTSLSEKIAV SEKRNSSEKK SVLEKTSVSE KSLAPGMALG
260 270 280 290 300
SGRRLVSEKA SIFEKALASE KSPTADAKPA PKRATASEQP LAQEPPASGG
310 320 330 340 350
SPATTKEQRG RALPGKNLPS LAKQGASDPP TVASRLPPVT LQVKIPSKEE
360 370 380 390 400
EADMSSPTQR TYSSSLKRSS PRTISFRMKP KKENSETTLT RSASMKLPDN
410 420 430 440 450
TVKLGEKLER YHTAIRRSES VKSRGLPCTE LFVAPVGVAS KRHLFEKELA
460 470 480 490 500
GQSRAEPASS RKENLRLSGV VTSRLNLWIS RTQESGDQDP QEAQKASSAT
510
ERTQWGQKSD SSLDAEV
Length:517
Mass (Da):57,131
Last modified:September 23, 2008 - v2
Checksum:i622E81B9412A924B
GO

Sequence cautioni

The sequence AAD10849.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti56 – 561A → S.
Corresponds to variant rs3738281 [ dbSNP | Ensembl ].
VAR_046539
Natural varianti155 – 1551A → P.2 Publications
Corresponds to variant rs1128316 [ dbSNP | Ensembl ].
VAR_046540
Natural varianti243 – 2431L → P.
Corresponds to variant rs12088790 [ dbSNP | Ensembl ].
VAR_046541
Natural varianti279 – 2791P → Q.
Corresponds to variant rs11805972 [ dbSNP | Ensembl ].
VAR_046542
Natural varianti323 – 3231K → E.
Corresponds to variant rs4128458 [ dbSNP | Ensembl ].
VAR_046543
Natural varianti503 – 5031T → S.
Corresponds to variant rs2275866 [ dbSNP | Ensembl ].
VAR_046544

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U42408 mRNA. Translation: AAB58817.1.
U58994 Genomic DNA. Translation: AAD10849.1. Sequence problems.
AK313388 mRNA. Translation: BAG36186.1.
BC009742 mRNA. Translation: AAH09742.1.
CCDSiCCDS1410.1.
RefSeqiNP_005549.2. NM_005558.3.
UniGeneiHs.519035.

Genome annotation databases

EnsembliENST00000391967; ENSP00000375829; ENSG00000159166.
GeneIDi3898.
KEGGihsa:3898.
UCSCiuc001gwm.3. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U42408 mRNA. Translation: AAB58817.1.
U58994 Genomic DNA. Translation: AAD10849.1. Sequence problems.
AK313388 mRNA. Translation: BAG36186.1.
BC009742 mRNA. Translation: AAH09742.1.
CCDSiCCDS1410.1.
RefSeqiNP_005549.2. NM_005558.3.
UniGeneiHs.519035.

3D structure databases

ProteinModelPortaliO00515.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi110095. 3 interactions.
IntActiO00515. 3 interactions.
MINTiMINT-3974996.
STRINGi9606.ENSP00000375829.

PTM databases

PhosphoSiteiO00515.

Polymorphism and mutation databases

BioMutaiLAD1.

Proteomic databases

MaxQBiO00515.
PaxDbiO00515.
PRIDEiO00515.

Protocols and materials databases

DNASUi3898.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000391967; ENSP00000375829; ENSG00000159166.
GeneIDi3898.
KEGGihsa:3898.
UCSCiuc001gwm.3. human.

Organism-specific databases

CTDi3898.
GeneCardsiGC01M201350.
HGNCiHGNC:6472. LAD1.
HPAiHPA007256.
HPA028732.
MIMi602314. gene.
neXtProtiNX_O00515.
PharmGKBiPA30263.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG84480.
GeneTreeiENSGT00390000005256.
HOGENOMiHOG000113274.
HOVERGENiHBG006240.
InParanoidiO00515.
PhylomeDBiO00515.
TreeFamiTF335896.

Miscellaneous databases

ChiTaRSiLAD1. human.
GeneWikiiLAD1.
GenomeRNAii3898.
NextBioi15305.
PROiO00515.
SOURCEiSearch...

Gene expression databases

BgeeiO00515.
CleanExiHS_LAD1.
ExpressionAtlasiO00515. baseline and differential.
GenevestigatoriO00515.

Family and domain databases

InterProiIPR017404. Ladinin_1.
[Graphical view]
PIRSFiPIRSF038144. Ladinin_1. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning of a gene (LAD) underlining linear IgA disease and encoding a novel anchoring filament of the basement membrane zone."
    Megahed M., Motoki K., McGrath J., LaForgia S., Uitto J.
    Submitted (DEC-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT PRO-155.
    Tissue: Skin.
  2. "Intron-exon organisation of ladinin gene, LAD."
    Megahed M., LaForgia S., Motoki K., McGrath J.A., Uitto J.
    Submitted (MAY-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT PRO-155.
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Trachea.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Placenta.
  5. "Cloning of the human linear IgA disease gene (LADA) encoding a novel anchoring filament protein, ladinin."
    Megahed M., Motoki K., McGrath J., LaForgia S., Uitto J.
    J. Invest. Dermatol. 106:832-832(1996)
    Cited for: CHARACTERIZATION.
  6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
    Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
    Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-38; SER-64; SER-121; SER-123 AND SER-394, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-38; SER-64; SER-356 AND SER-485, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiLAD1_HUMAN
AccessioniPrimary (citable) accession number: O00515
Secondary accession number(s): O95614, Q96GD8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: September 23, 2008
Last modified: May 27, 2015
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.