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Protein

Serine/threonine-protein kinase 25

Gene

STK25

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Oxidant stress-activated serine/threonine kinase that may play a role in the response to environmental stress. Targets to the Golgi apparatus where it appears to regulate protein transport events, cell adhesion, and polarity complexes important for cell migration.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Enzyme regulationi

Interaction with Golgi matrix protein GOLGA2 leads to autophosphorylation on Thr-174, possibly as a consequence of stabilization of dimer formation. The C-terminal non-catalytic region inhibits the kinase activity.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei49ATPPROSITE-ProRule annotation1
Active sitei140Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi26 – 34ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW
  • protein homodimerization activity Source: UniProtKB
  • protein kinase activity Source: ProtInc
  • receptor signaling protein serine/threonine kinase activity Source: GO_Central

GO - Biological processi

  • establishment of Golgi localization Source: UniProtKB
  • establishment or maintenance of cell polarity Source: Ensembl
  • Golgi localization Source: UniProtKB
  • Golgi reassembly Source: UniProtKB
  • intrinsic apoptotic signaling pathway in response to hydrogen peroxide Source: UniProtKB
  • positive regulation of axonogenesis Source: Ensembl
  • positive regulation of stress-activated MAPK cascade Source: UniProtKB
  • protein autophosphorylation Source: UniProtKB
  • protein phosphorylation Source: UniProtKB
  • response to hydrogen peroxide Source: UniProtKB
  • response to oxidative stress Source: ProtInc
  • signal transduction Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciZFISH:HS03929-MONOMER.
SignaLinkiO00506.
SIGNORiO00506.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase 25 (EC:2.7.11.1)
Alternative name(s):
Ste20-like kinase
Sterile 20/oxidant stress-response kinase 1
Short name:
SOK-1
Short name:
Ste20/oxidant stress response kinase 1
Gene namesi
Name:STK25
Synonyms:SOK1, YSK1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

HGNCiHGNC:11404. STK25.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • extracellular exosome Source: UniProtKB
  • Golgi membrane Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Golgi apparatus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi49K → R: Loss of kinase activity and autophosphorylation. 1 Publication1
Mutagenesisi158D → A: Loss of kinase activity. 1 Publication1

Organism-specific databases

DisGeNETi10494.
OpenTargetsiENSG00000115694.
PharmGKBiPA36211.

Chemistry databases

ChEMBLiCHEMBL5552.
GuidetoPHARMACOLOGYi2218.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000867131 – 426Serine/threonine-protein kinase 25Add BLAST426

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei174Phosphothreonine; by autocatalysis1 Publication1
Modified residuei278PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO00506.
MaxQBiO00506.
PaxDbiO00506.
PeptideAtlasiO00506.
PRIDEiO00506.

PTM databases

iPTMnetiO00506.
PhosphoSitePlusiO00506.
SwissPalmiO00506.

Expressioni

Tissue specificityi

Ubiquitously expressed. Highest levels are found in testis, large intestine, brain and stomach followed by heart and lung.

Gene expression databases

BgeeiENSG00000115694.
CleanExiHS_STK25.
ExpressionAtlasiO00506. baseline and differential.
GenevisibleiO00506. HS.

Organism-specific databases

HPAiHPA047147.

Interactioni

Subunit structurei

Homodimer. Interacts with CTTNBP2NL.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CAB39Q9Y3763EBI-618295,EBI-306905
CCDC36Q8IYA83EBI-618295,EBI-8638439
CEP70Q8NHQ13EBI-618295,EBI-739624
GOLGA2A0A0C4DGS54EBI-618295,EBI-11522202
GOLGA2Q083796EBI-618295,EBI-618309
Golga2Q628392EBI-618295,EBI-618335From a different organism.
PDCD10Q9BUL818EBI-618295,EBI-740195
STRNO438153EBI-618295,EBI-1046642
TRIM27P143733EBI-618295,EBI-719493
YWHAZP631042EBI-618295,EBI-347088

GO - Molecular functioni

  • protein homodimerization activity Source: UniProtKB

Protein-protein interaction databases

BioGridi115757. 53 interactors.
DIPiDIP-34269N.
IntActiO00506. 42 interactors.
MINTiMINT-2997826.
STRINGi9606.ENSP00000325748.

Chemistry databases

BindingDBiO00506.

Structurei

Secondary structure

1426
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni5 – 7Combined sources3
Helixi8 – 10Combined sources3
Helixi16 – 19Combined sources4
Beta strandi20 – 28Combined sources9
Beta strandi30 – 39Combined sources10
Turni40 – 42Combined sources3
Beta strandi45 – 52Combined sources8
Turni53 – 56Combined sources4
Helixi60 – 72Combined sources13
Beta strandi81 – 87Combined sources7
Beta strandi90 – 96Combined sources7
Helixi103 – 107Combined sources5
Helixi114 – 133Combined sources20
Helixi143 – 145Combined sources3
Beta strandi146 – 148Combined sources3
Beta strandi154 – 156Combined sources3
Beta strandi170 – 172Combined sources3
Helixi179 – 181Combined sources3
Helixi184 – 187Combined sources4
Helixi195 – 210Combined sources16
Turni214 – 217Combined sources4
Helixi220 – 226Combined sources7
Turni227 – 229Combined sources3
Helixi241 – 250Combined sources10
Helixi255 – 257Combined sources3
Helixi261 – 264Combined sources4
Helixi268 – 272Combined sources5
Helixi277 – 280Combined sources4
Helixi281 – 291Combined sources11
Helixi357 – 360Combined sources4
Helixi362 – 374Combined sources13
Turni375 – 377Combined sources3
Helixi381 – 396Combined sources16
Helixi400 – 414Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2XIKX-ray1.97A1-293[»]
3W8HX-ray2.43B355-426[»]
4NZWX-ray3.58B1-293[»]
ProteinModelPortaliO00506.
SMRiO00506.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini20 – 270Protein kinasePROSITE-ProRule annotationAdd BLAST251

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0201. Eukaryota.
ENOG410XP9G. LUCA.
GeneTreeiENSGT00810000125395.
HOGENOMiHOG000234203.
HOVERGENiHBG108518.
InParanoidiO00506.
KOiK08838.
OMAiLHGSQKP.
OrthoDBiEOG091G0F24.
PhylomeDBiO00506.
TreeFamiTF354217.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O00506-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAHLRGFANQ HSRVDPEELF TKLDRIGKGS FGEVYKGIDN HTKEVVAIKI
60 70 80 90 100
IDLEEAEDEI EDIQQEITVL SQCDSPYITR YFGSYLKSTK LWIIMEYLGG
110 120 130 140 150
GSALDLLKPG PLEETYIATI LREILKGLDY LHSERKIHRD IKAANVLLSE
160 170 180 190 200
QGDVKLADFG VAGQLTDTQI KRNTFVGTPF WMAPEVIKQS AYDFKADIWS
210 220 230 240 250
LGITAIELAK GEPPNSDLHP MRVLFLIPKN SPPTLEGQHS KPFKEFVEAC
260 270 280 290 300
LNKDPRFRPT AKELLKHKFI TRYTKKTSFL TELIDRYKRW KSEGHGEESS
310 320 330 340 350
SEDSDIDGEA EDGEQGPIWT FPPTIRPSPH SKLHKGTALH SSQKPAEPVK
360 370 380 390 400
RQPRSQCLST LVRPVFGELK EKHKQSGGSV GALEELENAF SLAEESCPGI
410 420
SDKLMVHLVE RVQRFSHNRN HLTSTR
Length:426
Mass (Da):48,112
Last modified:July 1, 1997 - v1
Checksum:i183CE5700FCEA716
GO
Isoform 2 (identifier: O00506-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     11-87: Missing.

Note: No experimental confirmation available.
Show »
Length:349
Mass (Da):39,266
Checksum:iF1BC727F54CB32A4
GO
Isoform 3 (identifier: O00506-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-94: Missing.

Note: No experimental confirmation available.
Show »
Length:332
Mass (Da):37,297
Checksum:iB4D6A56543812A3C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti347 – 348EP → DA in CAA67700 (PubMed:8887545).Curated2

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_05167464Q → H.Corresponds to variant rs34341643dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0546831 – 94Missing in isoform 3. 1 PublicationAdd BLAST94
Alternative sequenceiVSP_05439711 – 87Missing in isoform 2. 1 PublicationAdd BLAST77

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X99325 mRNA. Translation: CAA67700.1.
D63780 mRNA. Translation: BAA20420.1.
AK291808 mRNA. Translation: BAF84497.1.
AK291947 mRNA. Translation: BAF84636.1.
AK315966 mRNA. Translation: BAH14337.1.
BT019961 mRNA. Translation: AAV38764.1.
AC110299 Genomic DNA. Translation: AAY14683.1.
DQ093965 Genomic DNA. Translation: AAY88740.1.
CH471063 Genomic DNA. Translation: EAW71265.1.
BC007852 mRNA. Translation: AAH07852.1.
BC091505 mRNA. Translation: AAH91505.1.
CCDSiCCDS2549.1. [O00506-1]
CCDS63199.1. [O00506-3]
CCDS63200.1. [O00506-2]
PIRiS71886.
RefSeqiNP_001258906.1. NM_001271977.1. [O00506-1]
NP_001258907.1. NM_001271978.1. [O00506-1]
NP_001258908.1. NM_001271979.1. [O00506-2]
NP_001258909.1. NM_001271980.1. [O00506-2]
NP_001269234.1. NM_001282305.1. [O00506-3]
NP_001269236.1. NM_001282307.1. [O00506-3]
NP_001269237.1. NM_001282308.1. [O00506-3]
NP_006365.2. NM_006374.4. [O00506-1]
UniGeneiHs.516807.

Genome annotation databases

EnsembliENST00000316586; ENSP00000325748; ENSG00000115694. [O00506-1]
ENST00000401869; ENSP00000385687; ENSG00000115694. [O00506-1]
ENST00000403346; ENSP00000384162; ENSG00000115694. [O00506-1]
ENST00000405585; ENSP00000385541; ENSG00000115694. [O00506-2]
ENST00000405883; ENSP00000384444; ENSG00000115694. [O00506-2]
ENST00000535007; ENSP00000446008; ENSG00000115694. [O00506-3]
ENST00000543554; ENSP00000444886; ENSG00000115694. [O00506-3]
GeneIDi10494.
KEGGihsa:10494.
UCSCiuc002wbm.5. human. [O00506-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Web resourcesi

NIEHS-SNPs

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X99325 mRNA. Translation: CAA67700.1.
D63780 mRNA. Translation: BAA20420.1.
AK291808 mRNA. Translation: BAF84497.1.
AK291947 mRNA. Translation: BAF84636.1.
AK315966 mRNA. Translation: BAH14337.1.
BT019961 mRNA. Translation: AAV38764.1.
AC110299 Genomic DNA. Translation: AAY14683.1.
DQ093965 Genomic DNA. Translation: AAY88740.1.
CH471063 Genomic DNA. Translation: EAW71265.1.
BC007852 mRNA. Translation: AAH07852.1.
BC091505 mRNA. Translation: AAH91505.1.
CCDSiCCDS2549.1. [O00506-1]
CCDS63199.1. [O00506-3]
CCDS63200.1. [O00506-2]
PIRiS71886.
RefSeqiNP_001258906.1. NM_001271977.1. [O00506-1]
NP_001258907.1. NM_001271978.1. [O00506-1]
NP_001258908.1. NM_001271979.1. [O00506-2]
NP_001258909.1. NM_001271980.1. [O00506-2]
NP_001269234.1. NM_001282305.1. [O00506-3]
NP_001269236.1. NM_001282307.1. [O00506-3]
NP_001269237.1. NM_001282308.1. [O00506-3]
NP_006365.2. NM_006374.4. [O00506-1]
UniGeneiHs.516807.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2XIKX-ray1.97A1-293[»]
3W8HX-ray2.43B355-426[»]
4NZWX-ray3.58B1-293[»]
ProteinModelPortaliO00506.
SMRiO00506.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115757. 53 interactors.
DIPiDIP-34269N.
IntActiO00506. 42 interactors.
MINTiMINT-2997826.
STRINGi9606.ENSP00000325748.

Chemistry databases

BindingDBiO00506.
ChEMBLiCHEMBL5552.
GuidetoPHARMACOLOGYi2218.

PTM databases

iPTMnetiO00506.
PhosphoSitePlusiO00506.
SwissPalmiO00506.

Proteomic databases

EPDiO00506.
MaxQBiO00506.
PaxDbiO00506.
PeptideAtlasiO00506.
PRIDEiO00506.

Protocols and materials databases

DNASUi10494.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000316586; ENSP00000325748; ENSG00000115694. [O00506-1]
ENST00000401869; ENSP00000385687; ENSG00000115694. [O00506-1]
ENST00000403346; ENSP00000384162; ENSG00000115694. [O00506-1]
ENST00000405585; ENSP00000385541; ENSG00000115694. [O00506-2]
ENST00000405883; ENSP00000384444; ENSG00000115694. [O00506-2]
ENST00000535007; ENSP00000446008; ENSG00000115694. [O00506-3]
ENST00000543554; ENSP00000444886; ENSG00000115694. [O00506-3]
GeneIDi10494.
KEGGihsa:10494.
UCSCiuc002wbm.5. human. [O00506-1]

Organism-specific databases

CTDi10494.
DisGeNETi10494.
GeneCardsiSTK25.
HGNCiHGNC:11404. STK25.
HPAiHPA047147.
MIMi602255. gene.
neXtProtiNX_O00506.
OpenTargetsiENSG00000115694.
PharmGKBiPA36211.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0201. Eukaryota.
ENOG410XP9G. LUCA.
GeneTreeiENSGT00810000125395.
HOGENOMiHOG000234203.
HOVERGENiHBG108518.
InParanoidiO00506.
KOiK08838.
OMAiLHGSQKP.
OrthoDBiEOG091G0F24.
PhylomeDBiO00506.
TreeFamiTF354217.

Enzyme and pathway databases

BioCyciZFISH:HS03929-MONOMER.
SignaLinkiO00506.
SIGNORiO00506.

Miscellaneous databases

ChiTaRSiSTK25. human.
GeneWikiiSTK25.
GenomeRNAii10494.
PROiO00506.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000115694.
CleanExiHS_STK25.
ExpressionAtlasiO00506. baseline and differential.
GenevisibleiO00506. HS.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSTK25_HUMAN
AccessioniPrimary (citable) accession number: O00506
Secondary accession number(s): A8K6Z3
, A8K7D2, B7Z9K1, Q15522, Q5BJF1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: July 1, 1997
Last modified: November 30, 2016
This is version 173 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  7. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.