26S proteasome non-ATPase regulatory subunit 14

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Reviewed, UniProtKB/Swiss-Prot O00487 (PSDE_HUMAN)

Last modified June 16, 2009. Version 71. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    26S proteasome non-ATPase regulatory subunit 14
    EC=3.1.2.15
Alternative name(s):
    26S proteasome regulatory subunit rpn11
    26S proteasome-associated PAD1 homolog 1

Gene names

Name:	PSMD14
Synonyms:	POH1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length	310 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Metalloprotease component of the 26S proteasome that specifically cleaves 'Lys-63'-linked polyubiquitin chains. The 26S proteasome is involved in the ATP-dependent degradation of ubiquitinated proteins. The function of the 'Lys-63'-specific deubiquitination of the proteasome is unclear.
Catalytic activity	Ubiquitin C-terminal thioester + H₂O = ubiquitin + a thiol.
Subunit structure	Component of the 19S regulatory cap of the 26S proteasome.
Tissue specificity	Widely expressed. Highest levels in heart and skeletal muscle. Ref.1
Sequence similarities	Belongs to the peptidase M67A family. PSMD14 subfamily. Contains 1 MPN (JAB/Mov34) domain.

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Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Proteasome
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
PTM	Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process Inferred from Experiment. Source: Reactome negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle Inferred from Experiment. Source: Reactome positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle Inferred from Experiment. Source: Reactome protein K63-linked deubiquitination Ref.8 Traceable author statement. Source: UniProtKB
Cellular component	cytosol Inferred from electronic annotation. Source: UniProtKB-KW nucleus Inferred from direct assay. Source: HPA proteasome complex Ref.1 Ref.8 Traceable author statement. Source: UniProtKB
Molecular function	metallopeptidase activity Ref.8 Traceable author statement. Source: UniProtKB protein binding Inferred from physical interaction. Source: IntAct ubiquitin thiolesterase activity Ref.8 Traceable author statement. Source: UniProtKB zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct	Notes
PAAF1	Q9BRP4	1	EBI-722193,EBI-1056358
PSMD6	Q15008	1	EBI-722193,EBI-359701

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 310

310

26S proteasome non-ATPase regulatory subunit 14

PRO_0000213952

Regions

Domain

26 – 139

114

MPN

Motif

113 – 126

JAMM motif

Sites

Metal binding

113

Zinc; catalytic Probable

Metal binding

115

Zinc; catalytic Probable

Metal binding

126

Zinc; catalytic By similarity

Amino acid modifications

Modified residue

Phosphotyrosine Ref.4

Modified residue

150

Phosphoserine Ref.6

Modified residue

224

Phosphoserine Ref.5

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Sequences

Sequence

Length

Mass (Da)

Tools

O00487-1 [UniParc].

Last modified July 1, 1997. Version 1.
Checksum: 18ACE876C7682039
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FASTA

310

34,577

        10         20         30         40         50         60 
MDRLLRLGGG MPGLGQGPPT DAPAVDTAEQ VYISSLALLK MLKHGRAGVP MEVMGLMLGE 

        70         80         90        100        110        120 
FVDDYTVRVI DVFAMPQSGT GVSVEAVDPV FQAKMLDMLK QTGRPEMVVG WYHSHPGFGC 

       130        140        150        160        170        180 
WLSGVDINTQ QSFEALSERA VAVVVDPIQS VKGKVVIDAF RLINANMMVL GHEPRQTTSN 

       190        200        210        220        230        240 
LGHLNKPSIQ ALIHGLNRHY YSITINYRKN ELEQKMLLNL HKKSWMEGLT LQDYSEHCKH 

       250        260        270        280        290        300 
NESVVKEMLE LAKNYNKAVE EEDKMTPEQL AIKNVGKQDP KRHLEEHVDV LMTSNIVQCL 

       310 
AAMLDTVVFK

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Resistance to diverse drugs and ultraviolet light conferred by overexpression of a novel human 26 S proteasome subunit." Spataro V., Toda T., Craig R., Seeger M., Dubiel W., Harris A.L., Norbury C. J. Biol. Chem. 272:30470-30475(1997) [PubMed: 9374539] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY. Tissue: Lung.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
[3]	Lubec G., Afjehi-Sadat L. Submitted (MAR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 199-208, MASS SPECTROMETRY. Tissue: Brain and Cajal-Retzius cell.
[4]	"Immunoaffinity profiling of tyrosine phosphorylation in cancer cells." Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., Zhang H., Zha X.-M., Polakiewicz R.D., Comb M.J. Nat. Biotechnol. 23:94-101(2005) [PubMed: 15592455] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-32, MASS SPECTROMETRY.
[5]	"Mass spectrometric characterization of the affinity-purified human 26S proteasome complex." Wang X., Chen C.-F., Baker P.R., Chen P.-L., Kaiser P., Huang L. Biochemistry 46:3553-3565(2007) [PubMed: 17323924] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-224, MASS SPECTROMETRY.
[6]	"Quantitative phosphoproteome profiling of Wnt3a-mediated signaling network: indicating the involvement of ribonucleoside-diphosphate reductase M2 subunit phosphorylation at residue serine 20 in canonical Wnt signal transduction." Tang L.-Y., Deng N., Wang L.-S., Dai J., Wang Z.-L., Jiang X.-S., Li S.-J., Li L., Sheng Q.-H., Wu D.-Q., Li L., Zeng R. Mol. Cell. Proteomics 6:1952-1967(2007) [PubMed: 17693683] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-150, MASS SPECTROMETRY.
[7]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[8]	"K63-specific deubiquitination by two JAMM/MPN+ complexes: BRISC-associated Brcc36 and proteasomal Poh1." Cooper E.M., Cutcliffe C., Kristiansen T.Z., Pandey A., Pickart C.M., Cohen R.E. EMBO J. 28:621-631(2009) [PubMed: 19214193] [Abstract] Cited for: PROBABLE FUNCTION, IDENTIFICATION IN THE PROTEASOME.
+	Additional computationally mapped references.

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Cross-references

Sequence databases
	U86782 mRNA. Translation: AAC51866.1. BC066336 mRNA. Translation: AAH66336.1.
IPI	IPI00024821.
RefSeq	NP_005796.1.
UniGene	Hs.567410
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	O00487. 10 interactions.
Protein family/group databases
MEROPS	M67.001.
PTM databases
PhosphoSite	O00487.
2-D gel databases
OGP	O00487.
REPRODUCTION-2DPAGE	IPI00024821.
Proteomic databases
PeptideAtlas	O00487.
PRIDE	O00487.
Genome annotation databases
Ensembl	ENSG00000115233. Homo sapiens. [Contig view]
GeneID	10213.
KEGG	hsa:10213.
Organism-specific databases
GeneCards	GC02P161872.
HGNC	HGNC:16889. PSMD14.
HPA	HPA002114.
MIM	607173. gene.
PharmGKB	PA134957776.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	O00487.
HOVERGEN	O00487.
OMA	O00487. QDPKKHL.
Enzyme and pathway databases
Reactome	REACT_11045. Signaling by Wnt. REACT_13. Metabolism of amino acids. REACT_13635. Regulation of activated PAK-2p34 by proteasome mediated degradation. REACT_152. Cell Cycle, Mitotic. REACT_1538. Cell Cycle Checkpoints. REACT_383. DNA Replication. REACT_578. Apoptosis. REACT_6185. HIV Infection. REACT_6850. Cdc20:Phospho-APC/C mediated degradation of Cyclin A. REACT_9035. APC/C:Cdh1-mediated degradation of Skp2.
Gene expression databases
ArrayExpress	O00487.
Bgee	O00487.
CleanEx	HS_PSMD14.
GermOnline	ENSG00000115233. Homo sapiens.
Family and domain databases
InterPro	IPR000555. Mov34_MPN_PAD1. [Graphical view]
Pfam	PF01398. Mov34. 1 hit. [Graphical view]
ProDom	PD363422. Mov34-1. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00232. JAB_MPN. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	38668.
SOURCE	Search...

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Entry information

Entry name

PSDE_HUMAN

Accession

Primary (citable) accession number: O00487
Secondary accession number(s): O00176

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 31, 2004
Last sequence update:	July 1, 1997
Last modified:	June 16, 2009
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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Relevant documents

Human chromosome 2

Human chromosome 2: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Peptidase families

Classification of peptidase families and list of entries

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

PTM databases

2-D gel databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents