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Protein

Butyrophilin subfamily 3 member A1

Gene

BTN3A1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in T-cell activation and in the adaptive immune response. Regulates the proliferation of activated T-cells. Regulates the release of cytokines and IFNG by activated T-cells. Mediates the response of T-cells toward infected and transformed cells that are characterized by high levels of phosphorylated metabolites, such as isopentenyl pyrophosphate.4 Publications

GO - Biological processi

  • activated T cell proliferation Source: UniProtKB
  • adaptive immune response Source: UniProtKB-KW
  • cytokine secretion Source: UniProtKB
  • interferon-gamma secretion Source: UniProtKB
  • T cell receptor signaling pathway Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Adaptive immunity, Immunity

Names & Taxonomyi

Protein namesi
Recommended name:
Butyrophilin subfamily 3 member A1
Alternative name(s):
CD_antigen: CD277
Gene namesi
Name:BTN3A1
Synonyms:BTF5
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

HGNCiHGNC:1138. BTN3A1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini30 – 254ExtracellularSequence analysisAdd BLAST225
Transmembranei255 – 271HelicalSequence analysisAdd BLAST17
Topological domaini272 – 513CytoplasmicSequence analysisAdd BLAST242

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi11119.
OpenTargetsiENSG00000026950.
PharmGKBiPA25459.

Polymorphism and mutation databases

BioMutaiBTN3A1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 29Sequence analysisAdd BLAST29
ChainiPRO_000001453230 – 513Butyrophilin subfamily 3 member A1Add BLAST484

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi52 ↔ 126PROSITE-ProRule annotation1 Publication
Glycosylationi115N-linked (GlcNAc...)1 Publication1
Disulfide bondi166 ↔ 220PROSITE-ProRule annotation1 Publication

Post-translational modificationi

N-glycosylated.2 Publications

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiO00481.
PaxDbiO00481.
PeptideAtlasiO00481.
PRIDEiO00481.

PTM databases

iPTMnetiO00481.
PhosphoSitePlusiO00481.

Expressioni

Tissue specificityi

Detected on T-cells, natural killer cells, dendritic cells and macrophages (at protein level). Ubiquitous. Highly expressed in heart, pancreas and lung, Moderately expressed in placenta, liver and muscle.4 Publications

Gene expression databases

BgeeiENSG00000026950.
CleanExiHS_BTN3A1.
ExpressionAtlasiO00481. baseline and differential.
GenevisibleiO00481. HS.

Organism-specific databases

HPAiHPA012565.

Interactioni

Subunit structurei

Homodimer.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
TFF1P041553EBI-2809309,EBI-743871

Protein-protein interaction databases

BioGridi116294. 3 interactors.
IntActiO00481. 2 interactors.
STRINGi9606.ENSP00000289361.

Structurei

Secondary structure

1513
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi32 – 34Combined sources3
Beta strandi40 – 43Combined sources4
Beta strandi48 – 56Combined sources9
Beta strandi63 – 69Combined sources7
Turni70 – 73Combined sources4
Beta strandi74 – 80Combined sources7
Helixi86 – 88Combined sources3
Helixi91 – 93Combined sources3
Beta strandi96 – 100Combined sources5
Helixi104 – 106Combined sources3
Beta strandi108 – 115Combined sources8
Helixi118 – 120Combined sources3
Beta strandi122 – 130Combined sources9
Beta strandi133 – 143Combined sources11
Beta strandi151 – 158Combined sources8
Beta strandi161 – 173Combined sources13
Beta strandi176 – 180Combined sources5
Beta strandi202 – 210Combined sources9
Beta strandi218 – 224Combined sources7
Turni225 – 228Combined sources4
Beta strandi229 – 235Combined sources7
Turni239 – 241Combined sources3
Helixi328 – 336Combined sources9
Beta strandi337 – 339Combined sources3
Helixi346 – 348Combined sources3
Beta strandi353 – 355Combined sources3
Beta strandi359 – 364Combined sources6
Beta strandi379 – 381Combined sources3
Beta strandi383 – 387Combined sources5
Beta strandi390 – 400Combined sources11
Beta strandi407 – 413Combined sources7
Helixi426 – 428Combined sources3
Beta strandi430 – 436Combined sources7
Turni437 – 439Combined sources3
Beta strandi440 – 443Combined sources4
Beta strandi449 – 451Combined sources3
Beta strandi458 – 465Combined sources8
Turni466 – 469Combined sources4
Beta strandi470 – 475Combined sources6
Turni476 – 478Combined sources3
Beta strandi481 – 485Combined sources5
Beta strandi494 – 499Combined sources6
Beta strandi508 – 510Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4F80X-ray1.94A30-246[»]
4F9LX-ray3.14A/B30-246[»]
4F9PX-ray3.52A/B30-246[»]
4JKWX-ray2.01A28-143[»]
4K55X-ray1.91A28-143[»]
4N7IX-ray1.40A328-513[»]
4N7UX-ray1.46A328-513[»]
4V1PX-ray2.04A325-512[»]
ProteinModelPortaliO00481.
SMRiO00481.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini30 – 139Ig-like V-type 1Add BLAST110
Domaini145 – 236Ig-like V-type 2Add BLAST92
Domaini322 – 513B30.2/SPRYPROSITE-ProRule annotationAdd BLAST192

Sequence similaritiesi

Contains 1 B30.2/SPRY domain.PROSITE-ProRule annotation

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IJI4. Eukaryota.
ENOG410YA6W. LUCA.
GeneTreeiENSGT00760000118933.
HOGENOMiHOG000230860.
HOVERGENiHBG050747.
InParanoidiO00481.
KOiK06712.
OMAiNVQRKGW.
OrthoDBiEOG091G05N6.
PhylomeDBiO00481.
TreeFamiTF331083.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
InterProiIPR001870. B30.2/SPRY.
IPR003879. Butyrophylin.
IPR013320. ConA-like_dom.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
IPR006574. PRY.
IPR003877. SPRY_dom.
[Graphical view]
PfamiPF13765. PRY. 1 hit.
PF00622. SPRY. 1 hit.
PF07686. V-set. 1 hit.
[Graphical view]
PRINTSiPR01407. BUTYPHLNCDUF.
SMARTiSM00409. IG. 1 hit.
SM00406. IGv. 1 hit.
SM00589. PRY. 1 hit.
SM00449. SPRY. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 2 hits.
SSF49899. SSF49899. 1 hit.
PROSITEiPS50188. B302_SPRY. 1 hit.
PS50835. IG_LIKE. 2 hits.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O00481-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKMASFLAFL LLNFRVCLLL LQLLMPHSAQ FSVLGPSGPI LAMVGEDADL
60 70 80 90 100
PCHLFPTMSA ETMELKWVSS SLRQVVNVYA DGKEVEDRQS APYRGRTSIL
110 120 130 140 150
RDGITAGKAA LRIHNVTASD SGKYLCYFQD GDFYEKALVE LKVAALGSDL
160 170 180 190 200
HVDVKGYKDG GIHLECRSTG WYPQPQIQWS NNKGENIPTV EAPVVADGVG
210 220 230 240 250
LYAVAASVIM RGSSGEGVSC TIRSSLLGLE KTASISIADP FFRSAQRWIA
260 270 280 290 300
ALAGTLPVLL LLLGGAGYFL WQQQEEKKTQ FRKKKREQEL REMAWSTMKQ
310 320 330 340 350
EQSTRVKLLE ELRWRSIQYA SRGERHSAYN EWKKALFKPA DVILDPKTAN
360 370 380 390 400
PILLVSEDQR SVQRAKEPQD LPDNPERFNW HYCVLGCESF ISGRHYWEVE
410 420 430 440 450
VGDRKEWHIG VCSKNVQRKG WVKMTPENGF WTMGLTDGNK YRTLTEPRTN
460 470 480 490 500
LKLPKPPKKV GVFLDYETGD ISFYNAVDGS HIHTFLDVSF SEALYPVFRI
510
LTLEPTALTI CPA
Length:513
Mass (Da):57,677
Last modified:October 31, 2006 - v3
Checksum:i8D834D70526D1F6F
GO
Isoform 2 (identifier: O00481-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     340-352: ADVILDPKTANPI → GEEMLQMRLHFVK
     353-513: Missing.

Note: No experimental confirmation available.
Show »
Length:352
Mass (Da):39,397
Checksum:i274CA3723C653772
GO
Isoform 3 (identifier: O00481-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     340-513: ADVILDPKTA...EPTALTICPA → GPPIGQTQQQ...SWGPEEGGES

Note: No experimental confirmation available.
Show »
Length:378
Mass (Da):41,819
Checksum:i274B31308FA9B73D
GO
Isoform 4 (identifier: O00481-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     143-194: Missing.

Note: No experimental confirmation available.
Show »
Length:461
Mass (Da):52,019
Checksum:i72FE04DA9664783D
GO

Sequence cautioni

The sequence CAA69164 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti13N → S in BAG58563 (PubMed:14702039).Curated1
Sequence conflicti25M → T in BAD97194 (Ref. 4) Curated1
Sequence conflicti46E → K in BAG61334 (PubMed:14702039).Curated1
Sequence conflicti111L → F in CAA69164 (PubMed:9382921).Curated1
Sequence conflicti118A → G in CAA69164 (PubMed:9382921).Curated1
Sequence conflicti121 – 122SG → RW in CAA69164 (PubMed:9382921).Curated2
Sequence conflicti130D → G in BAG58563 (PubMed:14702039).Curated1
Sequence conflicti239D → R in CAA69164 (PubMed:9382921).Curated1
Sequence conflicti254G → R in AAB53430 (PubMed:9149941).Curated1
Sequence conflicti509T → S in AAB53430 (PubMed:9149941).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_06130515R → H.1 PublicationCorresponds to variant rs56161420dbSNPEnsembl.1
Natural variantiVAR_021170224S → N.1 PublicationCorresponds to variant rs1057933dbSNPEnsembl.1
Natural variantiVAR_061306282R → T.1 PublicationCorresponds to variant rs41266839dbSNPEnsembl.1
Natural variantiVAR_028788456P → T.1 PublicationCorresponds to variant rs4712990dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_045062143 – 194Missing in isoform 4. 1 PublicationAdd BLAST52
Alternative sequenceiVSP_042034340 – 513ADVIL…TICPA → GPPIGQTQQQTRGQGSPVAL SQESAQRTDSWGPEEGGES in isoform 3. 1 PublicationAdd BLAST174
Alternative sequenceiVSP_012714340 – 352ADVIL…TANPI → GEEMLQMRLHFVK in isoform 2. 1 PublicationAdd BLAST13
Alternative sequenceiVSP_012715353 – 513Missing in isoform 2. 1 PublicationAdd BLAST161

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y07827 mRNA. Translation: CAA69164.1. Different initiation.
U90552 mRNA. Translation: AAB53430.1.
AK223474 mRNA. Translation: BAD97194.1.
AK290193 mRNA. Translation: BAF82882.1.
AK295720 mRNA. Translation: BAG58563.1.
AK299327 mRNA. Translation: BAG61334.1.
AL021917 Genomic DNA. Translation: CAC03424.3.
AL021917 Genomic DNA. Translation: CAM28245.1.
CH471087 Genomic DNA. Translation: EAW55566.1.
CH471087 Genomic DNA. Translation: EAW55567.1.
BC118586 mRNA. Translation: AAI18587.1.
BC121800 mRNA. Translation: AAI21801.1.
CCDSiCCDS4608.1. [O00481-1]
CCDS4609.1. [O00481-2]
CCDS47388.1. [O00481-4]
CCDS47389.1. [O00481-3]
RefSeqiNP_001138480.1. NM_001145008.1. [O00481-4]
NP_001138481.1. NM_001145009.1. [O00481-3]
NP_008979.3. NM_007048.5. [O00481-1]
NP_919423.1. NM_194441.2. [O00481-2]
XP_005248891.1. XM_005248834.3. [O00481-2]
UniGeneiHs.191510.

Genome annotation databases

EnsembliENST00000289361; ENSP00000289361; ENSG00000026950. [O00481-1]
ENST00000414912; ENSP00000406667; ENSG00000026950. [O00481-4]
ENST00000425234; ENSP00000396684; ENSG00000026950. [O00481-3]
ENST00000476549; ENSP00000420010; ENSG00000026950. [O00481-2]
GeneIDi11119.
KEGGihsa:11119.
UCSCiuc003nhv.3. human. [O00481-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y07827 mRNA. Translation: CAA69164.1. Different initiation.
U90552 mRNA. Translation: AAB53430.1.
AK223474 mRNA. Translation: BAD97194.1.
AK290193 mRNA. Translation: BAF82882.1.
AK295720 mRNA. Translation: BAG58563.1.
AK299327 mRNA. Translation: BAG61334.1.
AL021917 Genomic DNA. Translation: CAC03424.3.
AL021917 Genomic DNA. Translation: CAM28245.1.
CH471087 Genomic DNA. Translation: EAW55566.1.
CH471087 Genomic DNA. Translation: EAW55567.1.
BC118586 mRNA. Translation: AAI18587.1.
BC121800 mRNA. Translation: AAI21801.1.
CCDSiCCDS4608.1. [O00481-1]
CCDS4609.1. [O00481-2]
CCDS47388.1. [O00481-4]
CCDS47389.1. [O00481-3]
RefSeqiNP_001138480.1. NM_001145008.1. [O00481-4]
NP_001138481.1. NM_001145009.1. [O00481-3]
NP_008979.3. NM_007048.5. [O00481-1]
NP_919423.1. NM_194441.2. [O00481-2]
XP_005248891.1. XM_005248834.3. [O00481-2]
UniGeneiHs.191510.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4F80X-ray1.94A30-246[»]
4F9LX-ray3.14A/B30-246[»]
4F9PX-ray3.52A/B30-246[»]
4JKWX-ray2.01A28-143[»]
4K55X-ray1.91A28-143[»]
4N7IX-ray1.40A328-513[»]
4N7UX-ray1.46A328-513[»]
4V1PX-ray2.04A325-512[»]
ProteinModelPortaliO00481.
SMRiO00481.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116294. 3 interactors.
IntActiO00481. 2 interactors.
STRINGi9606.ENSP00000289361.

PTM databases

iPTMnetiO00481.
PhosphoSitePlusiO00481.

Polymorphism and mutation databases

BioMutaiBTN3A1.

Proteomic databases

MaxQBiO00481.
PaxDbiO00481.
PeptideAtlasiO00481.
PRIDEiO00481.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000289361; ENSP00000289361; ENSG00000026950. [O00481-1]
ENST00000414912; ENSP00000406667; ENSG00000026950. [O00481-4]
ENST00000425234; ENSP00000396684; ENSG00000026950. [O00481-3]
ENST00000476549; ENSP00000420010; ENSG00000026950. [O00481-2]
GeneIDi11119.
KEGGihsa:11119.
UCSCiuc003nhv.3. human. [O00481-1]

Organism-specific databases

CTDi11119.
DisGeNETi11119.
GeneCardsiBTN3A1.
HGNCiHGNC:1138. BTN3A1.
HPAiHPA012565.
MIMi613593. gene.
neXtProtiNX_O00481.
OpenTargetsiENSG00000026950.
PharmGKBiPA25459.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IJI4. Eukaryota.
ENOG410YA6W. LUCA.
GeneTreeiENSGT00760000118933.
HOGENOMiHOG000230860.
HOVERGENiHBG050747.
InParanoidiO00481.
KOiK06712.
OMAiNVQRKGW.
OrthoDBiEOG091G05N6.
PhylomeDBiO00481.
TreeFamiTF331083.

Miscellaneous databases

ChiTaRSiBTN3A1. human.
GeneWikiiButyrophilin,_subfamily_3,_member_A1.
GenomeRNAii11119.
PROiO00481.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000026950.
CleanExiHS_BTN3A1.
ExpressionAtlasiO00481. baseline and differential.
GenevisibleiO00481. HS.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
InterProiIPR001870. B30.2/SPRY.
IPR003879. Butyrophylin.
IPR013320. ConA-like_dom.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
IPR006574. PRY.
IPR003877. SPRY_dom.
[Graphical view]
PfamiPF13765. PRY. 1 hit.
PF00622. SPRY. 1 hit.
PF07686. V-set. 1 hit.
[Graphical view]
PRINTSiPR01407. BUTYPHLNCDUF.
SMARTiSM00409. IG. 1 hit.
SM00406. IGv. 1 hit.
SM00589. PRY. 1 hit.
SM00449. SPRY. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 2 hits.
SSF49899. SSF49899. 1 hit.
PROSITEiPS50188. B302_SPRY. 1 hit.
PS50835. IG_LIKE. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBT3A1_HUMAN
AccessioniPrimary (citable) accession number: O00481
Secondary accession number(s): A2A278
, A8K2C8, B4DIQ1, B4DRM2, E9PGB4, E9PHG8, Q0P515, Q147X5, Q53F15, Q99420, Q9HCY1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 2005
Last sequence update: October 31, 2006
Last modified: November 2, 2016
This is version 147 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human cell differentiation molecules
    CD nomenclature of surface proteins of human leucocytes and list of entries
  2. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  7. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.