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O00478 (BT3A3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 126. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Butyrophilin subfamily 3 member A3
Gene names
Name:BTN3A3
Synonyms:BTF3
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length584 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Plays a role in T-cell responses in the adaptive immune response. Ref.10

Subunit structure

Homodimer. Ref.11

Subcellular location

Cell membrane; Single-pass type I membrane protein Ref.9 Ref.10.

Tissue specificity

Detected in peripheral blood mononuclear cells and in T-cells (at protein level). Detected in spleen and lymphocytes. Ref.8

Post-translational modification

N-glycosylated. Ref.8

Sequence similarities

Belongs to the immunoglobulin superfamily. BTN/MOG family.

Contains 1 B30.2/SPRY domain.

Contains 2 Ig-like V-type (immunoglobulin-like) domains.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: O00478-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: O00478-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-42: Missing.
     307-313: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2929 Ref.6
Chain30 – 584555Butyrophilin subfamily 3 member A3
PRO_0000014534

Regions

Topological domain30 – 248219Extracellular Potential
Transmembrane249 – 26921Helical; Potential
Topological domain270 – 584315Cytoplasmic Potential
Domain30 – 139110Ig-like V-type 1
Domain145 – 23692Ig-like V-type 2
Domain322 – 518197B30.2/SPRY

Amino acid modifications

Glycosylation1151N-linked (GlcNAc...) Ref.7
Disulfide bond52 ↔ 126 Ref.11
Disulfide bond166 ↔ 220 Ref.11

Natural variations

Alternative sequence1 – 4242Missing in isoform 2.
VSP_045063
Alternative sequence307 – 3137Missing in isoform 2.
VSP_045064

Experimental info

Sequence conflict2291L → P in BAG63049. Ref.3

Secondary structure

....................................... 584
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified July 1, 1997. Version 1.
Checksum: 2B279B9141E0327F

FASTA58465,002
        10         20         30         40         50         60 
MKMASSLAFL LLNFHVSLFL VQLLTPCSAQ FSVLGPSGPI LAMVGEDADL PCHLFPTMSA 

        70         80         90        100        110        120 
ETMELRWVSS SLRQVVNVYA DGKEVEDRQS APYRGRTSIL RDGITAGKAA LRIHNVTASD 

       130        140        150        160        170        180 
SGKYLCYFQD GDFYEKALVE LKVAALGSDL HIEVKGYEDG GIHLECRSTG WYPQPQIKWS 

       190        200        210        220        230        240 
DTKGENIPAV EAPVVADGVG LYAVAASVIM RGSSGGGVSC IIRNSLLGLE KTASISIADP 

       250        260        270        280        290        300 
FFRSAQPWIA ALAGTLPISL LLLAGASYFL WRQQKEKIAL SRETEREREM KEMGYAATEQ 

       310        320        330        340        350        360 
EISLREKLQE ELKWRKIQYM ARGEKSLAYH EWKMALFKPA DVILDPDTAN AILLVSEDQR 

       370        380        390        400        410        420 
SVQRAEEPRD LPDNPERFEW RYCVLGCENF TSGRHYWEVE VGDRKEWHIG VCSKNVERKK 

       430        440        450        460        470        480 
GWVKMTPENG YWTMGLTDGN KYRALTEPRT NLKLPEPPRK VGIFLDYETG EISFYNATDG 

       490        500        510        520        530        540 
SHIYTFPHAS FSEPLYPVFR ILTLEPTALT ICPIPKEVES SPDPDLVPDH SLETPLTPGL 

       550        560        570        580 
ANESGEPQAE VTSLLLPAHP GAEVSPSATT NQNHKLQART EALY 

« Hide

Isoform 2 [UniParc].

Checksum: 545FBB6D114FBB09
Show »

FASTA53559,688

References

« Hide 'large scale' references
[1]"A 1.1-Mb transcript map of the hereditary hemochromatosis locus."
Ruddy D.A., Kronmal G.S., Lee V.K., Mintier G.A., Quintana L., Domingo R. Jr., Meyer N.C., Irrinki A., McClelland E.E., Fullan A., Mapa F.A., Moore T., Thomas W., Loeb D.B., Harmon C., Tsuchihashi Z., Wolff R.K., Schatzman R.C., Feder J.N.
Genome Res. 7:441-456(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]"Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Mammary gland.
[4]"The DNA sequence and analysis of human chromosome 6."
Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. expand/collapse author list , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S.
Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Lymph.
[6]"Signal peptide prediction based on analysis of experimentally verified cleavage sites."
Zhang Z., Henzel W.J.
Protein Sci. 13:2819-2824(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 30-44.
[7]"Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-115.
Tissue: Leukemic T-cell.
[8]"Stimulation of human butyrophilin 3 molecules results in negative regulation of cellular immunity."
Yamashiro H., Yoshizaki S., Tadaki T., Egawa K., Seo N.
J. Leukoc. Biol. 88:757-767(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION, TISSUE SPECIFICITY.
[9]"Differential role for CD277 as a co-regulator of the immune signal in T and NK cells."
Messal N., Mamessier E., Sylvain A., Celis-Gutierrez J., Thibult M.L., Chetaille B., Firaguay G., Pastor S., Guillaume Y., Wang Q., Hirsch I., Nunes J.A., Olive D.
Eur. J. Immunol. 41:3443-3454(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[10]"Key implication of CD277/butyrophilin-3 (BTN3A) in cellular stress sensing by a major human gammadelta T-cell subset."
Harly C., Guillaume Y., Nedellec S., Peigne C.M., Monkkonen H., Monkkonen J., Li J., Kuball J., Adams E.J., Netzer S., Dechanet-Merville J., Leger A., Herrmann T., Breathnach R., Olive D., Bonneville M., Scotet E.
Blood 120:2269-2279(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[11]"The molecular basis for modulation of human Vgamma9Vdelta2 T cell responses by CD277/butyrophilin-3 (BTN3A)-specific antibodies."
Palakodeti A., Sandstrom A., Sundaresan L., Harly C., Nedellec S., Olive D., Scotet E., Bonneville M., Adams E.J.
J. Biol. Chem. 287:32780-32790(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.38 ANGSTROMS) OF 30-246, SUBUNIT, DISULFIDE BONDS.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U90548 mRNA. Translation: AAB53426.1.
BT007251 mRNA. Translation: AAP35915.1.
AK301553 mRNA. Translation: BAG63049.1.
AL021917 Genomic DNA. Translation: CAA17273.1.
BC015815 mRNA. Translation: AAH15815.1.
RefSeqNP_001229732.1. NM_001242803.1.
NP_008925.1. NM_006994.4.
NP_932078.2. NM_197974.2.
UniGeneHs.167741.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4F8TX-ray2.38A30-246[»]
ProteinModelPortalO00478.
SMRO00478. Positions 30-242, 341-514.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid115657. 3 interactions.
IntActO00478. 3 interactions.
STRING9606.ENSP00000244519.

PTM databases

PhosphoSiteO00478.

Proteomic databases

PaxDbO00478.
PRIDEO00478.

Protocols and materials databases

DNASU10384.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000244519; ENSP00000244519; ENSG00000111801. [O00478-1]
ENST00000361232; ENSP00000355238; ENSG00000111801. [O00478-2]
GeneID10384.
KEGGhsa:10384.
UCSCuc003nhz.3. human. [O00478-1]

Organism-specific databases

CTD10384.
GeneCardsGC06P026449.
HGNCHGNC:1140. BTN3A3.
HPAHPA007904.
HPA011871.
MIM613595. gene.
neXtProtNX_O00478.
PharmGKBPA25461.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG314774.
HOGENOMHOG000230860.
HOVERGENHBG050747.
InParanoidO00478.
KOK06712.
OMAERFNWHH.
OrthoDBEOG7QVM25.
PhylomeDBO00478.
TreeFamTF331083.

Gene expression databases

ArrayExpressO00478.
BgeeO00478.
CleanExHS_BTF3.
HS_BTN3A3.
GenevestigatorO00478.

Family and domain databases

Gene3D2.60.40.10. 2 hits.
InterProIPR001870. B30.2/SPRY.
IPR003879. Butyrophylin.
IPR008985. ConA-like_lec_gl_sf.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
IPR006574. PRY.
IPR018355. SPla/RYanodine_receptor_subgr.
IPR003877. SPRY_rcpt.
[Graphical view]
PfamPF13765. PRY. 1 hit.
PF00622. SPRY. 1 hit.
PF07686. V-set. 1 hit.
[Graphical view]
PRINTSPR01407. BUTYPHLNCDUF.
SMARTSM00409. IG. 1 hit.
SM00589. PRY. 1 hit.
SM00449. SPRY. 1 hit.
[Graphical view]
SUPFAMSSF49899. SSF49899. 1 hit.
PROSITEPS50188. B302_SPRY. 1 hit.
PS50835. IG_LIKE. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiBTN3A3.
GenomeRNAi10384.
NextBio39339.
PROO00478.
SOURCESearch...

Entry information

Entry nameBT3A3_HUMAN
AccessionPrimary (citable) accession number: O00478
Secondary accession number(s): B4DWI7, E9PCP5
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 2005
Last sequence update: July 1, 1997
Last modified: April 16, 2014
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 6

Human chromosome 6: entries, gene names and cross-references to MIM