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Protein

Butyrophilin subfamily 3 member A3

Gene

BTN3A3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in T-cell responses in the adaptive immune response.1 Publication

GO - Biological processi

  • T cell mediated immunity Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Adaptive immunity, Immunity

Enzyme and pathway databases

BioCyciZFISH:ENSG00000124561-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Butyrophilin subfamily 3 member A3
Gene namesi
Name:BTN3A3
Synonyms:BTF3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

HGNCiHGNC:1140. BTN3A3.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini30 – 248ExtracellularSequence analysisAdd BLAST219
Transmembranei249 – 269HelicalSequence analysisAdd BLAST21
Topological domaini270 – 584CytoplasmicSequence analysisAdd BLAST315

GO - Cellular componenti

  • cytoplasm Source: HPA
  • Golgi apparatus Source: HPA
  • integral component of membrane Source: UniProtKB-KW
  • membrane Source: UniProtKB
  • nucleolus Source: HPA
  • plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi10384.
OpenTargetsiENSG00000111801.
PharmGKBiPA25461.

Polymorphism and mutation databases

BioMutaiBTN3A3.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 291 PublicationAdd BLAST29
ChainiPRO_000001453430 – 584Butyrophilin subfamily 3 member A3Add BLAST555

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi52 ↔ 126PROSITE-ProRule annotation1 Publication
Glycosylationi115N-linked (GlcNAc...)1 Publication1
Disulfide bondi166 ↔ 220PROSITE-ProRule annotation1 Publication

Post-translational modificationi

N-glycosylated.2 Publications

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

EPDiO00478.
MaxQBiO00478.
PaxDbiO00478.
PeptideAtlasiO00478.
PRIDEiO00478.

PTM databases

iPTMnetiO00478.
PhosphoSitePlusiO00478.

Expressioni

Tissue specificityi

Detected in peripheral blood mononuclear cells and in T-cells (at protein level). Detected in spleen and lymphocytes.1 Publication

Gene expression databases

BgeeiENSG00000111801.
CleanExiHS_BTF3.
HS_BTN3A3.
ExpressionAtlasiO00478. baseline and differential.
GenevisibleiO00478. HS.

Organism-specific databases

HPAiHPA007904.
HPA011871.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

BioGridi115657. 5 interactors.
IntActiO00478. 3 interactors.
STRINGi9606.ENSP00000244519.

Structurei

Secondary structure

1584
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi32 – 34Combined sources3
Beta strandi40 – 43Combined sources4
Beta strandi48 – 56Combined sources9
Beta strandi63 – 69Combined sources7
Turni70 – 73Combined sources4
Beta strandi74 – 80Combined sources7
Helixi91 – 93Combined sources3
Beta strandi96 – 100Combined sources5
Helixi104 – 106Combined sources3
Beta strandi108 – 115Combined sources8
Helixi118 – 120Combined sources3
Beta strandi122 – 130Combined sources9
Beta strandi133 – 145Combined sources13
Beta strandi151 – 158Combined sources8
Beta strandi161 – 173Combined sources13
Beta strandi176 – 181Combined sources6
Beta strandi202 – 210Combined sources9
Beta strandi218 – 224Combined sources7
Turni225 – 228Combined sources4
Beta strandi229 – 235Combined sources7
Turni239 – 241Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4F8TX-ray2.38A30-246[»]
ProteinModelPortaliO00478.
SMRiO00478.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini30 – 139Ig-like V-type 1Add BLAST110
Domaini145 – 236Ig-like V-type 2Add BLAST92
Domaini322 – 518B30.2/SPRYPROSITE-ProRule annotationAdd BLAST197

Sequence similaritiesi

Contains 1 B30.2/SPRY domain.PROSITE-ProRule annotation

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IJI4. Eukaryota.
ENOG410YA6W. LUCA.
GeneTreeiENSGT00760000118933.
HOGENOMiHOG000230860.
HOVERGENiHBG050747.
InParanoidiO00478.
KOiK06712.
OMAiSSAYHEW.
OrthoDBiEOG091G05N6.
PhylomeDBiO00478.
TreeFamiTF331083.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
InterProiIPR001870. B30.2/SPRY.
IPR003879. Butyrophylin.
IPR013320. ConA-like_dom.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
IPR006574. PRY.
IPR003877. SPRY_dom.
[Graphical view]
PfamiPF13765. PRY. 1 hit.
PF00622. SPRY. 1 hit.
PF07686. V-set. 1 hit.
[Graphical view]
PRINTSiPR01407. BUTYPHLNCDUF.
SMARTiSM00409. IG. 1 hit.
SM00406. IGv. 1 hit.
SM00589. PRY. 1 hit.
SM00449. SPRY. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 2 hits.
SSF49899. SSF49899. 1 hit.
PROSITEiPS50188. B302_SPRY. 1 hit.
PS50835. IG_LIKE. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O00478-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKMASSLAFL LLNFHVSLFL VQLLTPCSAQ FSVLGPSGPI LAMVGEDADL
60 70 80 90 100
PCHLFPTMSA ETMELRWVSS SLRQVVNVYA DGKEVEDRQS APYRGRTSIL
110 120 130 140 150
RDGITAGKAA LRIHNVTASD SGKYLCYFQD GDFYEKALVE LKVAALGSDL
160 170 180 190 200
HIEVKGYEDG GIHLECRSTG WYPQPQIKWS DTKGENIPAV EAPVVADGVG
210 220 230 240 250
LYAVAASVIM RGSSGGGVSC IIRNSLLGLE KTASISIADP FFRSAQPWIA
260 270 280 290 300
ALAGTLPISL LLLAGASYFL WRQQKEKIAL SRETEREREM KEMGYAATEQ
310 320 330 340 350
EISLREKLQE ELKWRKIQYM ARGEKSLAYH EWKMALFKPA DVILDPDTAN
360 370 380 390 400
AILLVSEDQR SVQRAEEPRD LPDNPERFEW RYCVLGCENF TSGRHYWEVE
410 420 430 440 450
VGDRKEWHIG VCSKNVERKK GWVKMTPENG YWTMGLTDGN KYRALTEPRT
460 470 480 490 500
NLKLPEPPRK VGIFLDYETG EISFYNATDG SHIYTFPHAS FSEPLYPVFR
510 520 530 540 550
ILTLEPTALT ICPIPKEVES SPDPDLVPDH SLETPLTPGL ANESGEPQAE
560 570 580
VTSLLLPAHP GAEVSPSATT NQNHKLQART EALY
Length:584
Mass (Da):65,002
Last modified:July 1, 1997 - v1
Checksum:i2B279B9141E0327F
GO
Isoform 2 (identifier: O00478-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-42: Missing.
     307-313: Missing.

Note: No experimental confirmation available.
Show »
Length:535
Mass (Da):59,688
Checksum:i545FBB6D114FBB09
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti229L → P in BAG63049 (PubMed:14702039).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0450631 – 42Missing in isoform 2. 1 PublicationAdd BLAST42
Alternative sequenceiVSP_045064307 – 313Missing in isoform 2. 1 Publication7

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U90548 mRNA. Translation: AAB53426.1.
BT007251 mRNA. Translation: AAP35915.1.
AK301553 mRNA. Translation: BAG63049.1.
AL021917 Genomic DNA. Translation: CAA17273.1.
BC015815 mRNA. Translation: AAH15815.1.
CCDSiCCDS4611.1. [O00478-1]
CCDS4612.2. [O00478-2]
RefSeqiNP_001229732.1. NM_001242803.1.
NP_008925.1. NM_006994.4. [O00478-1]
NP_932078.2. NM_197974.2. [O00478-2]
UniGeneiHs.167741.

Genome annotation databases

EnsembliENST00000244519; ENSP00000244519; ENSG00000111801. [O00478-1]
ENST00000361232; ENSP00000355238; ENSG00000111801. [O00478-2]
GeneIDi10384.
KEGGihsa:10384.
UCSCiuc003nhz.3. human. [O00478-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U90548 mRNA. Translation: AAB53426.1.
BT007251 mRNA. Translation: AAP35915.1.
AK301553 mRNA. Translation: BAG63049.1.
AL021917 Genomic DNA. Translation: CAA17273.1.
BC015815 mRNA. Translation: AAH15815.1.
CCDSiCCDS4611.1. [O00478-1]
CCDS4612.2. [O00478-2]
RefSeqiNP_001229732.1. NM_001242803.1.
NP_008925.1. NM_006994.4. [O00478-1]
NP_932078.2. NM_197974.2. [O00478-2]
UniGeneiHs.167741.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4F8TX-ray2.38A30-246[»]
ProteinModelPortaliO00478.
SMRiO00478.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115657. 5 interactors.
IntActiO00478. 3 interactors.
STRINGi9606.ENSP00000244519.

PTM databases

iPTMnetiO00478.
PhosphoSitePlusiO00478.

Polymorphism and mutation databases

BioMutaiBTN3A3.

Proteomic databases

EPDiO00478.
MaxQBiO00478.
PaxDbiO00478.
PeptideAtlasiO00478.
PRIDEiO00478.

Protocols and materials databases

DNASUi10384.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000244519; ENSP00000244519; ENSG00000111801. [O00478-1]
ENST00000361232; ENSP00000355238; ENSG00000111801. [O00478-2]
GeneIDi10384.
KEGGihsa:10384.
UCSCiuc003nhz.3. human. [O00478-1]

Organism-specific databases

CTDi10384.
DisGeNETi10384.
GeneCardsiBTN3A3.
HGNCiHGNC:1140. BTN3A3.
HPAiHPA007904.
HPA011871.
MIMi613595. gene.
neXtProtiNX_O00478.
OpenTargetsiENSG00000111801.
PharmGKBiPA25461.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IJI4. Eukaryota.
ENOG410YA6W. LUCA.
GeneTreeiENSGT00760000118933.
HOGENOMiHOG000230860.
HOVERGENiHBG050747.
InParanoidiO00478.
KOiK06712.
OMAiSSAYHEW.
OrthoDBiEOG091G05N6.
PhylomeDBiO00478.
TreeFamiTF331083.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000124561-MONOMER.

Miscellaneous databases

GeneWikiiBTN3A3.
GenomeRNAii10384.
PROiO00478.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000111801.
CleanExiHS_BTF3.
HS_BTN3A3.
ExpressionAtlasiO00478. baseline and differential.
GenevisibleiO00478. HS.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
InterProiIPR001870. B30.2/SPRY.
IPR003879. Butyrophylin.
IPR013320. ConA-like_dom.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
IPR006574. PRY.
IPR003877. SPRY_dom.
[Graphical view]
PfamiPF13765. PRY. 1 hit.
PF00622. SPRY. 1 hit.
PF07686. V-set. 1 hit.
[Graphical view]
PRINTSiPR01407. BUTYPHLNCDUF.
SMARTiSM00409. IG. 1 hit.
SM00406. IGv. 1 hit.
SM00589. PRY. 1 hit.
SM00449. SPRY. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 2 hits.
SSF49899. SSF49899. 1 hit.
PROSITEiPS50188. B302_SPRY. 1 hit.
PS50835. IG_LIKE. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBT3A3_HUMAN
AccessioniPrimary (citable) accession number: O00478
Secondary accession number(s): B4DWI7, E9PCP5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 2005
Last sequence update: July 1, 1997
Last modified: November 2, 2016
This is version 148 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.