O00468 (AGRIN_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Agrin

Cleaved into the following 4 chains:

1. Agrin N-terminal 110 kDa subunit
2. Agrin C-terminal 110 kDa subunit
3. Agrin C-terminal 90 kDa fragment
   Short name=C90
4. Agrin C-terminal 22 kDa fragment
   Short name=C22

Gene names

Name:	AGRN
Synonyms:	AGRIN

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	2067 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Isoform 1: heparan sulfate basal lamina glycoprotein that plays a central role in the formation and the maintenance of the neuromuscular junction (NMJ) and directs key events in postsynaptic differentiation. Component of the AGRN-LRP4 receptor complex that induces the phosphorylation and activation of MUSK. The activation of MUSK in myotubes induces the formation of NMJ by regulating different processes including the transcription of specific genes and the clustering of AChR in the postsynaptic membrane. Calcium ions are required for maximal AChR clustering. AGRN function in neurons is highly regulated by alternative splicing, glycan binding and proteolytic processing. Modulates calcium ion homestasis in neurons, specifically by inducing an increase in cytoplasmic calcium ions. Functions differentially in the central nervous system (CNS) by inhibiting the alpha(3)-subtype of Na+/K+-ATPase and evoking depolarization at CNS synapses. This secreted isoform forms a bridge, after release from motor neurons, to basal lamina through binding laminin via the NtA domain. Ref.11 Ref.13 Isoform 2: transmembrane form that is the predominate form in neurons of the brain, induces dendritic filopodia and synapse formation in mature hippocampal neurons in large part due to the attached glycosaminoglycan chains and the action of Rho-family GTPases. Ref.11 Ref.13 Isoform 1, isoform 4 and isoform 5: neuron-specific (z+) isoforms that contain C-terminal insertions of 8-19 AA are potent activators of AChR clustering. Isoform 5, agrin (z+8), containing the 8-AA insert, forms a receptor complex in myotubules containing the neuronal AGRN, the muscle-specific kinase MUSK and LRP4, a member of the LDL receptor family. The splicing factors, NOVA1 and NOVA2, regulate AGRN splicing and production of the 'z' isoforms. Ref.11 Ref.13 Isoform 3 and isoform 6: lack any 'z' insert, are muscle-specific and may be involved in endothelial cell differentiation. Ref.11 Ref.13 Agrin N-terminal 110 kDa subunit: is involved in regulation of neurite outgrowth probably due to the presence of the glycosaminoglcan (GAG) side chains of heparan and chondroitin sulfate attached to the Ser/Thr- and Gly/Ser-rich regions. Also involved in modulation of growth factor signaling By similarity. Ref.11 Ref.13 Agrin C-terminal 22 kDa fragment: this released fragment is important for agrin signaling and to exert a maximal dendritic filopodia-inducing effect. All 'z' splice variants (z+) of this fragment also show an increase in the number of filopodia. Ref.11 Ref.13
Subunit structure	Monomer By similarity. Interacts (N-terminal subunit) with TGF-beta family members, BMP2 AND BMP4; the interactions inhibit the activity of these growth factors. Interacts with TGFB1; the interaction enhances the activity of TGFB1 By similarity. Component of the AGRN-LRP4 complex that consists of a tetramer of two AGRN-LRP4 heterodimers. Interacts (via the laminin G-like 3 domain) directly with LRP4; the interaction is required for activation of MUSK and clustering of AChR and requires the 'z8' insert present in the z(+8) isoforms. Interacts with DAG1; the interaction is influenced by cell surface glycosaminoglycans and by alternative splicing of AGRN. Ref.4 Ref.11 Ref.15
Subcellular location	Isoform 1: Secreted › extracellular space › extracellular matrix. Note: Synaptic basal lamina at the neuromuscular junction By similarity. Ref.10 Isoform 2: Cell junction › synapse. Cell membrane; Single-pass type II membrane protein Ref.10.
Tissue specificity	Expressed in basement membranes of lung and kidney. Muscle- and neuron-specific isoforms are found. Isoforms (y+) with the 4 AA insert and (z+8) isoforms with the 8 AA insert are all neuron-specific. Isoforms (z+11) are found in both neuronal and non-neuronal tissues. Ref.1 Ref.7 Ref.10
Domain	The NtA domain, absent in TM-agrin, is required for binding laminin and connecting to basal lamina. Both laminin G-like 2 (G2) and laminin G-like 3 (G3) domains are required for alpha-dystroglycan/DAG1 binding. G3 domain is required for C-terminal heparin, heparan sulfate and sialic acid binding By similarity.
Post-translational modification	Contains heparan and chondroitin sulfate chains and alpha-dystroglycan as well as N-linked and O-linked oligosaccharides. Glycosaminoglycans (GAGs), present in the N-terminal 110 kDa fragment, are required for induction of filopodia in hippocampal neurons. The first cluster (Gly/Ser-rich) for GAG attachment contains heparan sulfate (HS) chains and the second cluster (Ser/Thr-rich), contains chondroitin sulfate (CS) chains. Heparin and heparin sulfate binding in the G3 domain is independent of calcium ions. Binds heparin with a stoichiometry of 2:1. Binds sialic acid with a stoichiometry of 1:1 and binding requires calcium ions By similarity. At synaptic junctions, cleaved at two conserved sites, alpha and beta, by neurotrypsin. Cleavage at the alpha-site produces the agrin N-terminal 110-kDa subunit and the agrin C-terminal 110-kDa subunit. Further cleavage of agrin C-terminal 110-kDa subunit at the beta site produces the C-terminal fragments, agrin C-terminal 90 kDa fragment and agrin C-terminal 22 kDa fragment. Excessive cleavage at the beta-site releases large amounts of the agrin C-terminal 22 kDa fragment leading to destabilization at the neuromuscular junction (NMJ).
Involvement in disease	Myasthenia, limb-girdle, familial (LGM) [MIM:254300]: A congenital myasthenic syndrome characterized by a typical 'limb girdle' pattern of muscle weakness with small, simplified neuromuscular junctions but normal acetylcholine receptor and acetylcholinesterase function. Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.13 Ref.15
Miscellaneous	Cleaved C-terminal fragments may be used as a biomarker for sarcopenia, age-related progressive loss of skeletal muscle (Ref.12).
Sequence similarities	Contains 4 EGF-like domains. Contains 9 Kazal-like domains. Contains 2 laminin EGF-like domains. Contains 3 laminin G-like domains. Contains 1 NtA (N-terminal agrin) domain. Contains 1 SEA domain.
Caution	The unknown residue 'x' in the transmembrane isoform is probably a proline residue by similarity to mouse and rat sequences.

Ontologies

Keywords
Biological process	Differentiation
Cellular component	Cell junction Cell membrane Extracellular matrix Membrane Secreted Synapse
Coding sequence diversity	Alternative splicing Polymorphism
Disease	Congenital myasthenic syndrome Disease mutation
Domain	EGF-like domain Laminin EGF-like domain Repeat Signal Transmembrane Transmembrane helix
Ligand	Calcium
Molecular function	Developmental protein
PTM	Disulfide bond Glycoprotein Heparan sulfate Proteoglycan
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	G-protein coupled acetylcholine receptor signaling pathway Traceable author statement PubMed 9405491. Source: UniProtKB axon guidance Traceable author statement. Source: Reactome carbohydrate metabolic process Traceable author statement. Source: Reactome chondroitin sulfate metabolic process Traceable author statement. Source: Reactome clustering of voltage-gated sodium channels Traceable author statement PubMed 9405491. Source: UniProtKB glycosaminoglycan biosynthetic process Traceable author statement. Source: Reactome glycosaminoglycan catabolic process Traceable author statement. Source: Reactome positive regulation of Rho GTPase activity Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of filopodium assembly Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of synaptic growth at neuromuscular junction Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of transcription from RNA polymerase II promoter Inferred from sequence or structural similarity. Source: UniProtKB receptor clustering Inferred from direct assay PubMed 15340048. Source: UniProtKB synapse organization Traceable author statement Ref.1. Source: UniProtKB
Cellular_component	Golgi lumen Traceable author statement. Source: Reactome basal lamina Inferred from direct assay PubMed 9405491. Source: UniProtKB cell junction Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW lysosomal lumen Traceable author statement. Source: Reactome plasma membrane Traceable author statement. Source: Reactome synapse Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	calcium ion binding Inferred from sequence or structural similarity. Source: UniProtKB chondroitin sulfate binding Inferred from sequence or structural similarity. Source: UniProtKB dystroglycan binding Inferred from sequence or structural similarity. Source: UniProtKB heparan sulfate proteoglycan binding Inferred from sequence or structural similarity. Source: UniProtKB laminin binding Traceable author statement Ref.1. Source: UniProtKB sialic acid binding Inferred from sequence or structural similarity. Source: UniProtKB structural constituent of cytoskeleton Traceable author statement Ref.1. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ATXN7	O15265	2	EBI-947482,EBI-708350

Alternative products

This entry describes 7 isoforms produced by alternative splicing. [Align] [Select]

Note: Many isoforms may exist depending on the occurrence and length of inserts at the x, y or z splice site. Four 'z' isoforms can be produced with inserts of 0, 8, 11 or 19 AA. Isoforms differ in their acetylcholine receptor clustering activity and tissue specificity.

Isoform 1 (identifier: O00468-1)

Also known as: Secreted agrin; LN-agrin;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O00468-2)

Also known as: Transmembrane agrin; TM-agrin;

The sequence of this isoform differs from the canonical sequence as follows:
     1-104: Missing.
     105-154: NQVSTGDTRI...NLEEVEFCVE → MPXLAVARDT...FAVLLFLNNY

Note: Produced by usage of an alternative first exon.

Isoform 3 (identifier: O00468-3)

Also known as: Agrin z(0);

The sequence of this isoform differs from the canonical sequence as follows:
     1889-1906: Missing.

Isoform 4 (identifier: O00468-4)

Also known as: Agrin z(+11);

The sequence of this isoform differs from the canonical sequence as follows:
     1889-1896: Missing.

Isoform 5 (identifier: O00468-5)

Also known as: Agrin z(+8);

The sequence of this isoform differs from the canonical sequence as follows:
     1897-1906: Missing.

Isoform 6 (identifier: O00468-6)

Also known as: Agrin y(0)z(0);

The sequence of this isoform differs from the canonical sequence as follows:
     1752-1755: Missing.
     1889-1906: Missing.

Isoform 7 (identifier: O00468-7)

Also known as: y(0);

The sequence of this isoform differs from the canonical sequence as follows:
     1752-1755: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 29	29	Potential
	Chain	30 – 2067	2038	Agrin		PRO_0000007471
	Chain	30 – 1102	1073	Agrin N-terminal 110 kDa subunit By similarity		PRO_0000421613
	Chain	1103 – 2067	965	Agrin C-terminal 110 kDa subunit By similarity		PRO_0000421614
	Chain	1103 – 1863	761	Agrin C-terminal 90 kDa fragment By similarity		PRO_0000421615
	Chain	1864 – 2067	204	Agrin C-terminal 22 kDa fragment By similarity		PRO_0000421616
Regions
	Domain	30 – 157	128	NtA
	Domain	191 – 244	54	Kazal-like 1
	Domain	264 – 319	56	Kazal-like 2
	Domain	337 – 391	55	Kazal-like 3
	Domain	408 – 463	56	Kazal-like 4
	Domain	484 – 536	53	Kazal-like 5
	Domain	540 – 601	62	Kazal-like 6
	Domain	607 – 666	60	Kazal-like 7
	Domain	699 – 752	54	Kazal-like 8
	Domain	793 – 846	54	Laminin EGF-like 1
	Domain	847 – 893	47	Laminin EGF-like 2
	Domain	917 – 971	55	Kazal-like 9
	Domain	1130 – 1252	123	SEA
	Domain	1329 – 1367	39	EGF-like 1
	Domain	1372 – 1548	177	Laminin G-like 1
	Domain	1549 – 1586	38	EGF-like 2
	Domain	1588 – 1625	38	EGF-like 3
	Domain	1635 – 1822	188	Laminin G-like 2
	Domain	1818 – 1857	40	EGF-like 4
	Domain	1868 – 2064	197	Laminin G-like 3
	Calcium binding	1940 – 2008	69	By similarity
	Compositional bias	671 – 677	7	Gly/Ser-rich
	Compositional bias	974 – 1099	126	Ser/Thr-rich
	Compositional bias	1058 – 1097	40	Gly/Ser-rich
	Compositional bias	1254 – 1324	71	Ser/Thr-rich
Sites
	Site	1102 – 1103	2	Cleavage, alpha site; by neurotrypsin By similarity
	Site	1250	1	Alternative splice site to produce 'x' isoforms By similarity
	Site	1751	1	Alternative splice site to produce 'y' isoforms By similarity
	Site	1862	1	Critical for cleavage by neurotrypsin By similarity
	Site	1863 – 1864	2	Cleavage, beta site; by neurotrypsin By similarity
	Site	1888	1	Alternative splice site to produce 'z' isoforms By similarity
	Site	1892	1	Highly important for the agrin receptor complex activity of the 'z(8)' insert By similarity
Amino acid modifications
	Glycosylation	135	1	N-linked (GlcNAc...) Ref.9
	Glycosylation	250	1	N-linked (GlcNAc...) Potential
	Glycosylation	777	1	N-linked (GlcNAc...) Potential
	Glycosylation	932	1	N-linked (GlcNAc...) Potential
	Glycosylation	1835	1	O-linked (Fuc...) By similarity
	Disulfide bond	31 ↔ 103		By similarity
	Disulfide bond	152 ↔ 177		Or C-152 with C-183 By similarity
	Disulfide bond	793 ↔ 805		By similarity
	Disulfide bond	795 ↔ 812		By similarity
	Disulfide bond	814 ↔ 823		By similarity
	Disulfide bond	826 ↔ 844		By similarity
	Disulfide bond	847 ↔ 859		By similarity
	Disulfide bond	849 ↔ 866		By similarity
	Disulfide bond	868 ↔ 877		By similarity
	Disulfide bond	880 ↔ 891		By similarity
	Disulfide bond	1333 ↔ 1344		By similarity
	Disulfide bond	1338 ↔ 1355		By similarity
	Disulfide bond	1357 ↔ 1366		By similarity
	Disulfide bond	1519 ↔ 1548		By similarity
	Disulfide bond	1553 ↔ 1564		By similarity
	Disulfide bond	1558 ↔ 1574		By similarity
	Disulfide bond	1576 ↔ 1585		By similarity
	Disulfide bond	1592 ↔ 1603		By similarity
	Disulfide bond	1597 ↔ 1613		By similarity
	Disulfide bond	1615 ↔ 1624		By similarity
	Disulfide bond	1822 ↔ 1836		By similarity
	Disulfide bond	1830 ↔ 1845		By similarity
	Disulfide bond	1847 ↔ 1856		By similarity
	Disulfide bond	2038 ↔ 2064		By similarity
Natural variations
	Alternative sequence	1 – 104	104	Missing in isoform 2.		VSP_045753
	Alternative sequence	105 – 154	50	NQVST…EFCVE → MPXLAVARDTRQPAGASLLV RGFMVPCNACLILLATATLG FAVLLFLNNY in isoform 2.		VSP_045754
	Alternative sequence	1752 – 1755	4	Missing in isoform 6 and isoform 7.		VSP_045755
	Alternative sequence	1889 – 1906	18	Missing in isoform 3 and isoform 6.		VSP_045756
	Alternative sequence	1889 – 1896	8	Missing in isoform 4.		VSP_045757
	Alternative sequence	1897 – 1906	10	Missing in isoform 5.		VSP_045758
	Natural variant	23	1	V → L. Ref.13		VAR_068724
	Natural variant	58	1	D → N. Ref.13		VAR_068725
	Natural variant	105	1	N → I. Ref.13		VAR_068726
	Natural variant	267	1	T → M. Ref.13		VAR_068727
	Natural variant	375	1	A → S. Ref.13		VAR_068728
	Natural variant	728	1	E → V. Ref.13		VAR_068729
	Natural variant	852	1	Q → R. Ref.13		VAR_068730
	Natural variant	984	1	V → M. Ref.13		VAR_068731
	Natural variant	1088	1	L → F. Ref.13		VAR_068732
	Natural variant	1118	1	T → K. Ref.13		VAR_068733
	Natural variant	1135	1	Q → R. Ref.13		VAR_068734
	Natural variant	1240	1	P → L. Ref.13		VAR_068735
	Natural variant	1341	1	G → R. Ref.13		VAR_068736
	Natural variant	1451	1	P → L. Ref.13		VAR_068737
	Natural variant	1514	1	A → T. Ref.13		VAR_068738
	Natural variant	1565	1	Q → H. Ref.13		VAR_068739
	Natural variant	1666	1	V → I. Ref.13 Corresponds to variant rs17160775 [ dbSNP | Ensembl ].		VAR_048966
	Natural variant	1671	1	R → Q. Ref.13		VAR_068740
	Natural variant	1698	1	R → P. Ref.13		VAR_068741
	Natural variant	1709	1	G → R in LGM; results in disruption of the neuromuscular junction architecture; does not affect phosphorylation of MUSK; does not affect AChR clustering. Ref.13		VAR_068742
	Natural variant	1727	1	V → F in LGM; decreased AGRN-induced clustering of AChR by >100-fold and decreased phosphorylation of the MUSK receptor and AChR beta subunit by about 10-fold. Increased binding to alpha-dystroglycan. Ref.15		VAR_069066
	Natural variant	1734	1	R → H. Ref.13		VAR_068743
	Natural variant	1789	1	D → N. Ref.13		VAR_068744
	Natural variant	2045	1	G → V. Ref.13		VAR_068745
Experimental info
	Sequence conflict	343	1	L → R in AAB52917. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (Secreted agrin) (LN-agrin) [UniParc]. Last modified March 6, 2013. Version 5. Checksum: E7FAAB9AFB7B8039	FASTA	2,067	217,232
	10 20 30 40 50 60 MAGRSHPGPL RPLLPLLVVA ACVLPGAGGT CPERALERRE EEANVVLTGT VEEILNVDPV 70 80 90 100 110 120 QHTYSCKVRV WRYLKGKDLV ARESLLDGGN KVVISGFGDP LICDNQVSTG DTRIFFVNPA 130 140 150 160 170 180 PPYLWPAHKN ELMLNSSLMR ITLRNLEEVE FCVEDKPGTH FTPVPPTPPD ACRGMLCGFG 190 200 210 220 230 240 AVCEPNAEGP GRASCVCKKS PCPSVVAPVC GSDASTYSNE CELQRAQCSQ QRRIRLLSRG 250 260 270 280 290 300 PCGSRDPCSN VTCSFGSTCA RSADGLTASC LCPATCRGAP EGTVCGSDGA DYPGECQLLR 310 320 330 340 350 360 RACARQENVF KKFDGPCDPC QGALPDPSRS CRVNPRTRRP EMLLRPESCP ARQAPVCGDD 370 380 390 400 410 420 GVTYENDCVM GRSGAARGLL LQKVRSGQCQ GRDQCPEPCR FNAVCLSRRG RPRCSCDRVT 430 440 450 460 470 480 CDGAYRPVCA QDGRTYDSDC WRQQAECRQQ RAIPSKHQGP CDQAPSPCLG VQCAFGATCA 490 500 510 520 530 540 VKNGQAACEC LQACSSLYDP VCGSDGVTYG SACELEATAC TLGREIQVAR KGPCDRCGQC 550 560 570 580 590 600 RFGALCEAET GRCVCPSECV ALAQPVCGSD GHTYPSECML HVHACTHQIS LHVASAGPCE 610 620 630 640 650 660 TCGDAVCAFG AVCSAGQCVC PRCEHPPPGP VCGSDGVTYG SACELREAAC LQQTQIEEAR 670 680 690 700 710 720 AGPCEQAECG SGGSGSGEDG DCEQELCRQR GGIWDEDSED GPCVCDFSCQ SVPGSPVCGS 730 740 750 760 770 780 DGVTYSTECE LKKARCESQR GLYVAAQGAC RGPTFAPLPP VAPLHCAQTP YGCCQDNITA 790 800 810 820 830 840 ARGVGLAGCP SACQCNPHGS YGGTCDPATG QCSCRPGVGG LRCDRCEPGF WNFRGIVTDG 850 860 870 880 890 900 RSGCTPCSCD PQGAVRDDCE QMTGLCSCKP GVAGPKCGQC PDGRALGPAG CEADASAPAT 910 920 930 940 950 960 CAEMRCEFGA RCVEESGSAH CVCPMLTCPE ANATKVCGSD GVTYGNECQL KTIACRQGLQ 970 980 990 1000 1010 1020 ISIQSLGPCQ EAVAPSTHPT SASVTVTTPG LLLSQALPAP PGALPLAPSS TAHSQTTPPP 1030 1040 1050 1060 1070 1080 SSRPRTTASV PRTTVWPVLT VPPTAPSPAP SLVASAFGES GSTDGSSDEE LSGDQEASGG 1090 1100 1110 1120 1130 1140 GSGGLEPLEG SSVATPGPPV ERASCYNSAL GCCSDGKTPS LDAEGSNCPA TKVFQGVLEL 1150 1160 1170 1180 1190 1200 EGVEGQELFY TPEMADPKSE LFGETARSIE STLDDLFRNS DVKKDFRSVR LRDLGPGKSV 1210 1220 1230 1240 1250 1260 RAIVDVHFDP TTAFRAPDVA RALLRQIQVS RRRSLGVRRP LQEHVRFMDF DWFPAFITGA 1270 1280 1290 1300 1310 1320 TSGAIAAGAT ARATTASRLP SSAVTPRAPH PSHTSQPVAK TTAAPTTRRP PTTAPSRVPG 1330 1340 1350 1360 1370 1380 RRPPAPQQPP KPCDSQPCFH GGTCQDWALG GGFTCSCPAG RGGAVCEKVL GAPVPAFEGR 1390 1400 1410 1420 1430 1440 SFLAFPTLRA YHTLRLALEF RALEPQGLLL YNGNARGKDF LALALLDGRV QLRFDTGSGP 1450 1460 1470 1480 1490 1500 AVLTSAVPVE PGQWHRLELS RHWRRGTLSV DGETPVLGES PSGTDGLNLD TDLFVGGVPE 1510 1520 1530 1540 1550 1560 DQAAVALERT FVGAGLRGCI RLLDVNNQRL ELGIGPGAAT RGSGVGECGD HPCLPNPCHG 1570 1580 1590 1600 1610 1620 GAPCQNLEAG RFHCQCPPGR VGPTCADEKS PCQPNPCHGA APCRVLPEGG AQCECPLGRE 1630 1640 1650 1660 1670 1680 GTFCQTASGQ DGSGPFLADF NGFSHLELRG LHTFARDLGE KMALEVVFLA RGPSGLLLYN 1690 1700 1710 1720 1730 1740 GQKTDGKGDF VSLALRDRRL EFRYDLGKGA AVIRSREPVT LGAWTRVSLE RNGRKGALRV 1750 1760 1770 1780 1790 1800 GDGPRVLGES PKSRKVPHTV LNLKEPLYVG GAPDFSKLAR AAAVSSGFDG AIQLVSLGGR 1810 1820 1830 1840 1850 1860 QLLTPEHVLR QVDVTSFAGH PCTRASGHPC LNGASCVPRE AAYVCLCPGG FSGPHCEKGL 1870 1880 1890 1900 1910 1920 VEKSAGDVDT LAFDGRTFVE YLNAVTESEL ANEIPVPETL DSGALHEKAL QSNHFELSLR 1930 1940 1950 1960 1970 1980 TEATQGLVLW SGKATERADY VALAIVDGHL QLSYNLGSQP VVLRSTVPVN TNRWLRVVAH 1990 2000 2010 2020 2030 2040 REQREGSLQV GNEAPVTGSS PLGATQLDTD GALWLGGLPE LPVGPALPKA YGTGFVGCLR 2050 2060 DVVVGRHPLH LLEDAVTKPE LRPCPTP
	Isoform 2 (Transmembrane agrin) (TM-agrin) [UniParc]. Checksum: 8E904C7A584AE78F	FASTA	1,963	205,544
	10 20 30 40 50 60 MPXLAVARDT RQPAGASLLV RGFMVPCNAC LILLATATLG FAVLLFLNNY DKPGTHFTPV 70 80 90 100 110 120 PPTPPDACRG MLCGFGAVCE PNAEGPGRAS CVCKKSPCPS VVAPVCGSDA STYSNECELQ 130 140 150 160 170 180 RAQCSQQRRI RLLSRGPCGS RDPCSNVTCS FGSTCARSAD GLTASCLCPA TCRGAPEGTV 190 200 210 220 230 240 CGSDGADYPG ECQLLRRACA RQENVFKKFD GPCDPCQGAL PDPSRSCRVN PRTRRPEMLL 250 260 270 280 290 300 RPESCPARQA PVCGDDGVTY ENDCVMGRSG AARGLLLQKV RSGQCQGRDQ CPEPCRFNAV 310 320 330 340 350 360 CLSRRGRPRC SCDRVTCDGA YRPVCAQDGR TYDSDCWRQQ AECRQQRAIP SKHQGPCDQA 370 380 390 400 410 420 PSPCLGVQCA FGATCAVKNG QAACECLQAC SSLYDPVCGS DGVTYGSACE LEATACTLGR 430 440 450 460 470 480 EIQVARKGPC DRCGQCRFGA LCEAETGRCV CPSECVALAQ PVCGSDGHTY PSECMLHVHA 490 500 510 520 530 540 CTHQISLHVA SAGPCETCGD AVCAFGAVCS AGQCVCPRCE HPPPGPVCGS DGVTYGSACE 550 560 570 580 590 600 LREAACLQQT QIEEARAGPC EQAECGSGGS GSGEDGDCEQ ELCRQRGGIW DEDSEDGPCV 610 620 630 640 650 660 CDFSCQSVPG SPVCGSDGVT YSTECELKKA RCESQRGLYV AAQGACRGPT FAPLPPVAPL 670 680 690 700 710 720 HCAQTPYGCC QDNITAARGV GLAGCPSACQ CNPHGSYGGT CDPATGQCSC RPGVGGLRCD 730 740 750 760 770 780 RCEPGFWNFR GIVTDGRSGC TPCSCDPQGA VRDDCEQMTG LCSCKPGVAG PKCGQCPDGR 790 800 810 820 830 840 ALGPAGCEAD ASAPATCAEM RCEFGARCVE ESGSAHCVCP MLTCPEANAT KVCGSDGVTY 850 860 870 880 890 900 GNECQLKTIA CRQGLQISIQ SLGPCQEAVA PSTHPTSASV TVTTPGLLLS QALPAPPGAL 910 920 930 940 950 960 PLAPSSTAHS QTTPPPSSRP RTTASVPRTT VWPVLTVPPT APSPAPSLVA SAFGESGSTD 970 980 990 1000 1010 1020 GSSDEELSGD QEASGGGSGG LEPLEGSSVA TPGPPVERAS CYNSALGCCS DGKTPSLDAE 1030 1040 1050 1060 1070 1080 GSNCPATKVF QGVLELEGVE GQELFYTPEM ADPKSELFGE TARSIESTLD DLFRNSDVKK 1090 1100 1110 1120 1130 1140 DFRSVRLRDL GPGKSVRAIV DVHFDPTTAF RAPDVARALL RQIQVSRRRS LGVRRPLQEH 1150 1160 1170 1180 1190 1200 VRFMDFDWFP AFITGATSGA IAAGATARAT TASRLPSSAV TPRAPHPSHT SQPVAKTTAA 1210 1220 1230 1240 1250 1260 PTTRRPPTTA PSRVPGRRPP APQQPPKPCD SQPCFHGGTC QDWALGGGFT CSCPAGRGGA 1270 1280 1290 1300 1310 1320 VCEKVLGAPV PAFEGRSFLA FPTLRAYHTL RLALEFRALE PQGLLLYNGN ARGKDFLALA 1330 1340 1350 1360 1370 1380 LLDGRVQLRF DTGSGPAVLT SAVPVEPGQW HRLELSRHWR RGTLSVDGET PVLGESPSGT 1390 1400 1410 1420 1430 1440 DGLNLDTDLF VGGVPEDQAA VALERTFVGA GLRGCIRLLD VNNQRLELGI GPGAATRGSG 1450 1460 1470 1480 1490 1500 VGECGDHPCL PNPCHGGAPC QNLEAGRFHC QCPPGRVGPT CADEKSPCQP NPCHGAAPCR 1510 1520 1530 1540 1550 1560 VLPEGGAQCE CPLGREGTFC QTASGQDGSG PFLADFNGFS HLELRGLHTF ARDLGEKMAL 1570 1580 1590 1600 1610 1620 EVVFLARGPS GLLLYNGQKT DGKGDFVSLA LRDRRLEFRY DLGKGAAVIR SREPVTLGAW 1630 1640 1650 1660 1670 1680 TRVSLERNGR KGALRVGDGP RVLGESPKSR KVPHTVLNLK EPLYVGGAPD FSKLARAAAV 1690 1700 1710 1720 1730 1740 SSGFDGAIQL VSLGGRQLLT PEHVLRQVDV TSFAGHPCTR ASGHPCLNGA SCVPREAAYV 1750 1760 1770 1780 1790 1800 CLCPGGFSGP HCEKGLVEKS AGDVDTLAFD GRTFVEYLNA VTESELANEI PVPETLDSGA 1810 1820 1830 1840 1850 1860 LHEKALQSNH FELSLRTEAT QGLVLWSGKA TERADYVALA IVDGHLQLSY NLGSQPVVLR 1870 1880 1890 1900 1910 1920 STVPVNTNRW LRVVAHREQR EGSLQVGNEA PVTGSSPLGA TQLDTDGALW LGGLPELPVG 1930 1940 1950 1960 PALPKAYGTG FVGCLRDVVV GRHPLHLLED AVTKPELRPC PTP
	Isoform 3 (Agrin z(0)) [UniParc]. Checksum: C6CC67CA63060E92	FASTA	2,049	215,345
	10 20 30 40 50 60 MAGRSHPGPL RPLLPLLVVA ACVLPGAGGT CPERALERRE EEANVVLTGT VEEILNVDPV 70 80 90 100 110 120 QHTYSCKVRV WRYLKGKDLV ARESLLDGGN KVVISGFGDP LICDNQVSTG DTRIFFVNPA 130 140 150 160 170 180 PPYLWPAHKN ELMLNSSLMR ITLRNLEEVE FCVEDKPGTH FTPVPPTPPD ACRGMLCGFG 190 200 210 220 230 240 AVCEPNAEGP GRASCVCKKS PCPSVVAPVC GSDASTYSNE CELQRAQCSQ QRRIRLLSRG 250 260 270 280 290 300 PCGSRDPCSN VTCSFGSTCA RSADGLTASC LCPATCRGAP EGTVCGSDGA DYPGECQLLR 310 320 330 340 350 360 RACARQENVF KKFDGPCDPC QGALPDPSRS CRVNPRTRRP EMLLRPESCP ARQAPVCGDD 370 380 390 400 410 420 GVTYENDCVM GRSGAARGLL LQKVRSGQCQ GRDQCPEPCR FNAVCLSRRG RPRCSCDRVT 430 440 450 460 470 480 CDGAYRPVCA QDGRTYDSDC WRQQAECRQQ RAIPSKHQGP CDQAPSPCLG VQCAFGATCA 490 500 510 520 530 540 VKNGQAACEC LQACSSLYDP VCGSDGVTYG SACELEATAC TLGREIQVAR KGPCDRCGQC 550 560 570 580 590 600 RFGALCEAET GRCVCPSECV ALAQPVCGSD GHTYPSECML HVHACTHQIS LHVASAGPCE 610 620 630 640 650 660 TCGDAVCAFG AVCSAGQCVC PRCEHPPPGP VCGSDGVTYG SACELREAAC LQQTQIEEAR 670 680 690 700 710 720 AGPCEQAECG SGGSGSGEDG DCEQELCRQR GGIWDEDSED GPCVCDFSCQ SVPGSPVCGS 730 740 750 760 770 780 DGVTYSTECE LKKARCESQR GLYVAAQGAC RGPTFAPLPP VAPLHCAQTP YGCCQDNITA 790 800 810 820 830 840 ARGVGLAGCP SACQCNPHGS YGGTCDPATG QCSCRPGVGG LRCDRCEPGF WNFRGIVTDG 850 860 870 880 890 900 RSGCTPCSCD PQGAVRDDCE QMTGLCSCKP GVAGPKCGQC PDGRALGPAG CEADASAPAT 910 920 930 940 950 960 CAEMRCEFGA RCVEESGSAH CVCPMLTCPE ANATKVCGSD GVTYGNECQL KTIACRQGLQ 970 980 990 1000 1010 1020 ISIQSLGPCQ EAVAPSTHPT SASVTVTTPG LLLSQALPAP PGALPLAPSS TAHSQTTPPP 1030 1040 1050 1060 1070 1080 SSRPRTTASV PRTTVWPVLT VPPTAPSPAP SLVASAFGES GSTDGSSDEE LSGDQEASGG 1090 1100 1110 1120 1130 1140 GSGGLEPLEG SSVATPGPPV ERASCYNSAL GCCSDGKTPS LDAEGSNCPA TKVFQGVLEL 1150 1160 1170 1180 1190 1200 EGVEGQELFY TPEMADPKSE LFGETARSIE STLDDLFRNS DVKKDFRSVR LRDLGPGKSV 1210 1220 1230 1240 1250 1260 RAIVDVHFDP TTAFRAPDVA RALLRQIQVS RRRSLGVRRP LQEHVRFMDF DWFPAFITGA 1270 1280 1290 1300 1310 1320 TSGAIAAGAT ARATTASRLP SSAVTPRAPH PSHTSQPVAK TTAAPTTRRP PTTAPSRVPG 1330 1340 1350 1360 1370 1380 RRPPAPQQPP KPCDSQPCFH GGTCQDWALG GGFTCSCPAG RGGAVCEKVL GAPVPAFEGR 1390 1400 1410 1420 1430 1440 SFLAFPTLRA YHTLRLALEF RALEPQGLLL YNGNARGKDF LALALLDGRV QLRFDTGSGP 1450 1460 1470 1480 1490 1500 AVLTSAVPVE PGQWHRLELS RHWRRGTLSV DGETPVLGES PSGTDGLNLD TDLFVGGVPE 1510 1520 1530 1540 1550 1560 DQAAVALERT FVGAGLRGCI RLLDVNNQRL ELGIGPGAAT RGSGVGECGD HPCLPNPCHG 1570 1580 1590 1600 1610 1620 GAPCQNLEAG RFHCQCPPGR VGPTCADEKS PCQPNPCHGA APCRVLPEGG AQCECPLGRE 1630 1640 1650 1660 1670 1680 GTFCQTASGQ DGSGPFLADF NGFSHLELRG LHTFARDLGE KMALEVVFLA RGPSGLLLYN 1690 1700 1710 1720 1730 1740 GQKTDGKGDF VSLALRDRRL EFRYDLGKGA AVIRSREPVT LGAWTRVSLE RNGRKGALRV 1750 1760 1770 1780 1790 1800 GDGPRVLGES PKSRKVPHTV LNLKEPLYVG GAPDFSKLAR AAAVSSGFDG AIQLVSLGGR 1810 1820 1830 1840 1850 1860 QLLTPEHVLR QVDVTSFAGH PCTRASGHPC LNGASCVPRE AAYVCLCPGG FSGPHCEKGL 1870 1880 1890 1900 1910 1920 VEKSAGDVDT LAFDGRTFVE YLNAVTESEK ALQSNHFELS LRTEATQGLV LWSGKATERA 1930 1940 1950 1960 1970 1980 DYVALAIVDG HLQLSYNLGS QPVVLRSTVP VNTNRWLRVV AHREQREGSL QVGNEAPVTG 1990 2000 2010 2020 2030 2040 SSPLGATQLD TDGALWLGGL PELPVGPALP KAYGTGFVGC LRDVVVGRHP LHLLEDAVTK PELRPCPTP
	Isoform 4 (Agrin z(+11)) [UniParc]. Checksum: 45560978CFB636D3	FASTA	2,059	216,366
	10 20 30 40 50 60 MAGRSHPGPL RPLLPLLVVA ACVLPGAGGT CPERALERRE EEANVVLTGT VEEILNVDPV 70 80 90 100 110 120 QHTYSCKVRV WRYLKGKDLV ARESLLDGGN KVVISGFGDP LICDNQVSTG DTRIFFVNPA 130 140 150 160 170 180 PPYLWPAHKN ELMLNSSLMR ITLRNLEEVE FCVEDKPGTH FTPVPPTPPD ACRGMLCGFG 190 200 210 220 230 240 AVCEPNAEGP GRASCVCKKS PCPSVVAPVC GSDASTYSNE CELQRAQCSQ QRRIRLLSRG 250 260 270 280 290 300 PCGSRDPCSN VTCSFGSTCA RSADGLTASC LCPATCRGAP EGTVCGSDGA DYPGECQLLR 310 320 330 340 350 360 RACARQENVF KKFDGPCDPC QGALPDPSRS CRVNPRTRRP EMLLRPESCP ARQAPVCGDD 370 380 390 400 410 420 GVTYENDCVM GRSGAARGLL LQKVRSGQCQ GRDQCPEPCR FNAVCLSRRG RPRCSCDRVT 430 440 450 460 470 480 CDGAYRPVCA QDGRTYDSDC WRQQAECRQQ RAIPSKHQGP CDQAPSPCLG VQCAFGATCA 490 500 510 520 530 540 VKNGQAACEC LQACSSLYDP VCGSDGVTYG SACELEATAC TLGREIQVAR KGPCDRCGQC 550 560 570 580 590 600 RFGALCEAET GRCVCPSECV ALAQPVCGSD GHTYPSECML HVHACTHQIS LHVASAGPCE 610 620 630 640 650 660 TCGDAVCAFG AVCSAGQCVC PRCEHPPPGP VCGSDGVTYG SACELREAAC LQQTQIEEAR 670 680 690 700 710 720 AGPCEQAECG SGGSGSGEDG DCEQELCRQR GGIWDEDSED GPCVCDFSCQ SVPGSPVCGS 730 740 750 760 770 780 DGVTYSTECE LKKARCESQR GLYVAAQGAC RGPTFAPLPP VAPLHCAQTP YGCCQDNITA 790 800 810 820 830 840 ARGVGLAGCP SACQCNPHGS YGGTCDPATG QCSCRPGVGG LRCDRCEPGF WNFRGIVTDG 850 860 870 880 890 900 RSGCTPCSCD PQGAVRDDCE QMTGLCSCKP GVAGPKCGQC PDGRALGPAG CEADASAPAT 910 920 930 940 950 960 CAEMRCEFGA RCVEESGSAH CVCPMLTCPE ANATKVCGSD GVTYGNECQL KTIACRQGLQ 970 980 990 1000 1010 1020 ISIQSLGPCQ EAVAPSTHPT SASVTVTTPG LLLSQALPAP PGALPLAPSS TAHSQTTPPP 1030 1040 1050 1060 1070 1080 SSRPRTTASV PRTTVWPVLT VPPTAPSPAP SLVASAFGES GSTDGSSDEE LSGDQEASGG 1090 1100 1110 1120 1130 1140 GSGGLEPLEG SSVATPGPPV ERASCYNSAL GCCSDGKTPS LDAEGSNCPA TKVFQGVLEL 1150 1160 1170 1180 1190 1200 EGVEGQELFY TPEMADPKSE LFGETARSIE STLDDLFRNS DVKKDFRSVR LRDLGPGKSV 1210 1220 1230 1240 1250 1260 RAIVDVHFDP TTAFRAPDVA RALLRQIQVS RRRSLGVRRP LQEHVRFMDF DWFPAFITGA 1270 1280 1290 1300 1310 1320 TSGAIAAGAT ARATTASRLP SSAVTPRAPH PSHTSQPVAK TTAAPTTRRP PTTAPSRVPG 1330 1340 1350 1360 1370 1380 RRPPAPQQPP KPCDSQPCFH GGTCQDWALG GGFTCSCPAG RGGAVCEKVL GAPVPAFEGR 1390 1400 1410 1420 1430 1440 SFLAFPTLRA YHTLRLALEF RALEPQGLLL YNGNARGKDF LALALLDGRV QLRFDTGSGP 1450 1460 1470 1480 1490 1500 AVLTSAVPVE PGQWHRLELS RHWRRGTLSV DGETPVLGES PSGTDGLNLD TDLFVGGVPE 1510 1520 1530 1540 1550 1560 DQAAVALERT FVGAGLRGCI RLLDVNNQRL ELGIGPGAAT RGSGVGECGD HPCLPNPCHG 1570 1580 1590 1600 1610 1620 GAPCQNLEAG RFHCQCPPGR VGPTCADEKS PCQPNPCHGA APCRVLPEGG AQCECPLGRE 1630 1640 1650 1660 1670 1680 GTFCQTASGQ DGSGPFLADF NGFSHLELRG LHTFARDLGE KMALEVVFLA RGPSGLLLYN 1690 1700 1710 1720 1730 1740 GQKTDGKGDF VSLALRDRRL EFRYDLGKGA AVIRSREPVT LGAWTRVSLE RNGRKGALRV 1750 1760 1770 1780 1790 1800 GDGPRVLGES PKSRKVPHTV LNLKEPLYVG GAPDFSKLAR AAAVSSGFDG AIQLVSLGGR 1810 1820 1830 1840 1850 1860 QLLTPEHVLR QVDVTSFAGH PCTRASGHPC LNGASCVPRE AAYVCLCPGG FSGPHCEKGL 1870 1880 1890 1900 1910 1920 VEKSAGDVDT LAFDGRTFVE YLNAVTESPE TLDSGALHEK ALQSNHFELS LRTEATQGLV 1930 1940 1950 1960 1970 1980 LWSGKATERA DYVALAIVDG HLQLSYNLGS QPVVLRSTVP VNTNRWLRVV AHREQREGSL 1990 2000 2010 2020 2030 2040 QVGNEAPVTG SSPLGATQLD TDGALWLGGL PELPVGPALP KAYGTGFVGC LRDVVVGRHP 2050 LHLLEDAVTK PELRPCPTP
	Isoform 5 (Agrin z(+8)) [UniParc]. Checksum: 099A9FB384BDE301	FASTA	2,057	216,211
	10 20 30 40 50 60 MAGRSHPGPL RPLLPLLVVA ACVLPGAGGT CPERALERRE EEANVVLTGT VEEILNVDPV 70 80 90 100 110 120 QHTYSCKVRV WRYLKGKDLV ARESLLDGGN KVVISGFGDP LICDNQVSTG DTRIFFVNPA 130 140 150 160 170 180 PPYLWPAHKN ELMLNSSLMR ITLRNLEEVE FCVEDKPGTH FTPVPPTPPD ACRGMLCGFG 190 200 210 220 230 240 AVCEPNAEGP GRASCVCKKS PCPSVVAPVC GSDASTYSNE CELQRAQCSQ QRRIRLLSRG 250 260 270 280 290 300 PCGSRDPCSN VTCSFGSTCA RSADGLTASC LCPATCRGAP EGTVCGSDGA DYPGECQLLR 310 320 330 340 350 360 RACARQENVF KKFDGPCDPC QGALPDPSRS CRVNPRTRRP EMLLRPESCP ARQAPVCGDD 370 380 390 400 410 420 GVTYENDCVM GRSGAARGLL LQKVRSGQCQ GRDQCPEPCR FNAVCLSRRG RPRCSCDRVT 430 440 450 460 470 480 CDGAYRPVCA QDGRTYDSDC WRQQAECRQQ RAIPSKHQGP CDQAPSPCLG VQCAFGATCA 490 500 510 520 530 540 VKNGQAACEC LQACSSLYDP VCGSDGVTYG SACELEATAC TLGREIQVAR KGPCDRCGQC 550 560 570 580 590 600 RFGALCEAET GRCVCPSECV ALAQPVCGSD GHTYPSECML HVHACTHQIS LHVASAGPCE 610 620 630 640 650 660 TCGDAVCAFG AVCSAGQCVC PRCEHPPPGP VCGSDGVTYG SACELREAAC LQQTQIEEAR 670 680 690 700 710 720 AGPCEQAECG SGGSGSGEDG DCEQELCRQR GGIWDEDSED GPCVCDFSCQ SVPGSPVCGS 730 740 750 760 770 780 DGVTYSTECE LKKARCESQR GLYVAAQGAC RGPTFAPLPP VAPLHCAQTP YGCCQDNITA 790 800 810 820 830 840 ARGVGLAGCP SACQCNPHGS YGGTCDPATG QCSCRPGVGG LRCDRCEPGF WNFRGIVTDG 850 860 870 880 890 900 RSGCTPCSCD PQGAVRDDCE QMTGLCSCKP GVAGPKCGQC PDGRALGPAG CEADASAPAT 910 920 930 940 950 960 CAEMRCEFGA RCVEESGSAH CVCPMLTCPE ANATKVCGSD GVTYGNECQL KTIACRQGLQ 970 980 990 1000 1010 1020 ISIQSLGPCQ EAVAPSTHPT SASVTVTTPG LLLSQALPAP PGALPLAPSS TAHSQTTPPP 1030 1040 1050 1060 1070 1080 SSRPRTTASV PRTTVWPVLT VPPTAPSPAP SLVASAFGES GSTDGSSDEE LSGDQEASGG 1090 1100 1110 1120 1130 1140 GSGGLEPLEG SSVATPGPPV ERASCYNSAL GCCSDGKTPS LDAEGSNCPA TKVFQGVLEL 1150 1160 1170 1180 1190 1200 EGVEGQELFY TPEMADPKSE LFGETARSIE STLDDLFRNS DVKKDFRSVR LRDLGPGKSV 1210 1220 1230 1240 1250 1260 RAIVDVHFDP TTAFRAPDVA RALLRQIQVS RRRSLGVRRP LQEHVRFMDF DWFPAFITGA 1270 1280 1290 1300 1310 1320 TSGAIAAGAT ARATTASRLP SSAVTPRAPH PSHTSQPVAK TTAAPTTRRP PTTAPSRVPG 1330 1340 1350 1360 1370 1380 RRPPAPQQPP KPCDSQPCFH GGTCQDWALG GGFTCSCPAG RGGAVCEKVL GAPVPAFEGR 1390 1400 1410 1420 1430 1440 SFLAFPTLRA YHTLRLALEF RALEPQGLLL YNGNARGKDF LALALLDGRV QLRFDTGSGP 1450 1460 1470 1480 1490 1500 AVLTSAVPVE PGQWHRLELS RHWRRGTLSV DGETPVLGES PSGTDGLNLD TDLFVGGVPE 1510 1520 1530 1540 1550 1560 DQAAVALERT FVGAGLRGCI RLLDVNNQRL ELGIGPGAAT RGSGVGECGD HPCLPNPCHG 1570 1580 1590 1600 1610 1620 GAPCQNLEAG RFHCQCPPGR VGPTCADEKS PCQPNPCHGA APCRVLPEGG AQCECPLGRE 1630 1640 1650 1660 1670 1680 GTFCQTASGQ DGSGPFLADF NGFSHLELRG LHTFARDLGE KMALEVVFLA RGPSGLLLYN 1690 1700 1710 1720 1730 1740 GQKTDGKGDF VSLALRDRRL EFRYDLGKGA AVIRSREPVT LGAWTRVSLE RNGRKGALRV 1750 1760 1770 1780 1790 1800 GDGPRVLGES PKSRKVPHTV LNLKEPLYVG GAPDFSKLAR AAAVSSGFDG AIQLVSLGGR 1810 1820 1830 1840 1850 1860 QLLTPEHVLR QVDVTSFAGH PCTRASGHPC LNGASCVPRE AAYVCLCPGG FSGPHCEKGL 1870 1880 1890 1900 1910 1920 VEKSAGDVDT LAFDGRTFVE YLNAVTESEL ANEIPVEKAL QSNHFELSLR TEATQGLVLW 1930 1940 1950 1960 1970 1980 SGKATERADY VALAIVDGHL QLSYNLGSQP VVLRSTVPVN TNRWLRVVAH REQREGSLQV 1990 2000 2010 2020 2030 2040 GNEAPVTGSS PLGATQLDTD GALWLGGLPE LPVGPALPKA YGTGFVGCLR DVVVGRHPLH 2050 LLEDAVTKPE LRPCPTP
	Isoform 6 (Agrin y(0)z(0)) [UniParc]. Checksum: 108BDA7146ECB94D	FASTA	2,045	214,846
	10 20 30 40 50 60 MAGRSHPGPL RPLLPLLVVA ACVLPGAGGT CPERALERRE EEANVVLTGT VEEILNVDPV 70 80 90 100 110 120 QHTYSCKVRV WRYLKGKDLV ARESLLDGGN KVVISGFGDP LICDNQVSTG DTRIFFVNPA 130 140 150 160 170 180 PPYLWPAHKN ELMLNSSLMR ITLRNLEEVE FCVEDKPGTH FTPVPPTPPD ACRGMLCGFG 190 200 210 220 230 240 AVCEPNAEGP GRASCVCKKS PCPSVVAPVC GSDASTYSNE CELQRAQCSQ QRRIRLLSRG 250 260 270 280 290 300 PCGSRDPCSN VTCSFGSTCA RSADGLTASC LCPATCRGAP EGTVCGSDGA DYPGECQLLR 310 320 330 340 350 360 RACARQENVF KKFDGPCDPC QGALPDPSRS CRVNPRTRRP EMLLRPESCP ARQAPVCGDD 370 380 390 400 410 420 GVTYENDCVM GRSGAARGLL LQKVRSGQCQ GRDQCPEPCR FNAVCLSRRG RPRCSCDRVT 430 440 450 460 470 480 CDGAYRPVCA QDGRTYDSDC WRQQAECRQQ RAIPSKHQGP CDQAPSPCLG VQCAFGATCA 490 500 510 520 530 540 VKNGQAACEC LQACSSLYDP VCGSDGVTYG SACELEATAC TLGREIQVAR KGPCDRCGQC 550 560 570 580 590 600 RFGALCEAET GRCVCPSECV ALAQPVCGSD GHTYPSECML HVHACTHQIS LHVASAGPCE 610 620 630 640 650 660 TCGDAVCAFG AVCSAGQCVC PRCEHPPPGP VCGSDGVTYG SACELREAAC LQQTQIEEAR 670 680 690 700 710 720 AGPCEQAECG SGGSGSGEDG DCEQELCRQR GGIWDEDSED GPCVCDFSCQ SVPGSPVCGS 730 740 750 760 770 780 DGVTYSTECE LKKARCESQR GLYVAAQGAC RGPTFAPLPP VAPLHCAQTP YGCCQDNITA 790 800 810 820 830 840 ARGVGLAGCP SACQCNPHGS YGGTCDPATG QCSCRPGVGG LRCDRCEPGF WNFRGIVTDG 850 860 870 880 890 900 RSGCTPCSCD PQGAVRDDCE QMTGLCSCKP GVAGPKCGQC PDGRALGPAG CEADASAPAT 910 920 930 940 950 960 CAEMRCEFGA RCVEESGSAH CVCPMLTCPE ANATKVCGSD GVTYGNECQL KTIACRQGLQ 970 980 990 1000 1010 1020 ISIQSLGPCQ EAVAPSTHPT SASVTVTTPG LLLSQALPAP PGALPLAPSS TAHSQTTPPP 1030 1040 1050 1060 1070 1080 SSRPRTTASV PRTTVWPVLT VPPTAPSPAP SLVASAFGES GSTDGSSDEE LSGDQEASGG 1090 1100 1110 1120 1130 1140 GSGGLEPLEG SSVATPGPPV ERASCYNSAL GCCSDGKTPS LDAEGSNCPA TKVFQGVLEL 1150 1160 1170 1180 1190 1200 EGVEGQELFY TPEMADPKSE LFGETARSIE STLDDLFRNS DVKKDFRSVR LRDLGPGKSV 1210 1220 1230 1240 1250 1260 RAIVDVHFDP TTAFRAPDVA RALLRQIQVS RRRSLGVRRP LQEHVRFMDF DWFPAFITGA 1270 1280 1290 1300 1310 1320 TSGAIAAGAT ARATTASRLP SSAVTPRAPH PSHTSQPVAK TTAAPTTRRP PTTAPSRVPG 1330 1340 1350 1360 1370 1380 RRPPAPQQPP KPCDSQPCFH GGTCQDWALG GGFTCSCPAG RGGAVCEKVL GAPVPAFEGR 1390 1400 1410 1420 1430 1440 SFLAFPTLRA YHTLRLALEF RALEPQGLLL YNGNARGKDF LALALLDGRV QLRFDTGSGP 1450 1460 1470 1480 1490 1500 AVLTSAVPVE PGQWHRLELS RHWRRGTLSV DGETPVLGES PSGTDGLNLD TDLFVGGVPE 1510 1520 1530 1540 1550 1560 DQAAVALERT FVGAGLRGCI RLLDVNNQRL ELGIGPGAAT RGSGVGECGD HPCLPNPCHG 1570 1580 1590 1600 1610 1620 GAPCQNLEAG RFHCQCPPGR VGPTCADEKS PCQPNPCHGA APCRVLPEGG AQCECPLGRE 1630 1640 1650 1660 1670 1680 GTFCQTASGQ DGSGPFLADF NGFSHLELRG LHTFARDLGE KMALEVVFLA RGPSGLLLYN 1690 1700 1710 1720 1730 1740 GQKTDGKGDF VSLALRDRRL EFRYDLGKGA AVIRSREPVT LGAWTRVSLE RNGRKGALRV 1750 1760 1770 1780 1790 1800 GDGPRVLGES PVPHTVLNLK EPLYVGGAPD FSKLARAAAV SSGFDGAIQL VSLGGRQLLT 1810 1820 1830 1840 1850 1860 PEHVLRQVDV TSFAGHPCTR ASGHPCLNGA SCVPREAAYV CLCPGGFSGP HCEKGLVEKS 1870 1880 1890 1900 1910 1920 AGDVDTLAFD GRTFVEYLNA VTESEKALQS NHFELSLRTE ATQGLVLWSG KATERADYVA 1930 1940 1950 1960 1970 1980 LAIVDGHLQL SYNLGSQPVV LRSTVPVNTN RWLRVVAHRE QREGSLQVGN EAPVTGSSPL 1990 2000 2010 2020 2030 2040 GATQLDTDGA LWLGGLPELP VGPALPKAYG TGFVGCLRDV VVGRHPLHLL EDAVTKPELR PCPTP
	Isoform 7 (y(0)) [UniParc]. Checksum: 9E761313F0E53B1B	FASTA	2,063	216,733
	10 20 30 40 50 60 MAGRSHPGPL RPLLPLLVVA ACVLPGAGGT CPERALERRE EEANVVLTGT VEEILNVDPV 70 80 90 100 110 120 QHTYSCKVRV WRYLKGKDLV ARESLLDGGN KVVISGFGDP LICDNQVSTG DTRIFFVNPA 130 140 150 160 170 180 PPYLWPAHKN ELMLNSSLMR ITLRNLEEVE FCVEDKPGTH FTPVPPTPPD ACRGMLCGFG 190 200 210 220 230 240 AVCEPNAEGP GRASCVCKKS PCPSVVAPVC GSDASTYSNE CELQRAQCSQ QRRIRLLSRG 250 260 270 280 290 300 PCGSRDPCSN VTCSFGSTCA RSADGLTASC LCPATCRGAP EGTVCGSDGA DYPGECQLLR 310 320 330 340 350 360 RACARQENVF KKFDGPCDPC QGALPDPSRS CRVNPRTRRP EMLLRPESCP ARQAPVCGDD 370 380 390 400 410 420 GVTYENDCVM GRSGAARGLL LQKVRSGQCQ GRDQCPEPCR FNAVCLSRRG RPRCSCDRVT 430 440 450 460 470 480 CDGAYRPVCA QDGRTYDSDC WRQQAECRQQ RAIPSKHQGP CDQAPSPCLG VQCAFGATCA 490 500 510 520 530 540 VKNGQAACEC LQACSSLYDP VCGSDGVTYG SACELEATAC TLGREIQVAR KGPCDRCGQC 550 560 570 580 590 600 RFGALCEAET GRCVCPSECV ALAQPVCGSD GHTYPSECML HVHACTHQIS LHVASAGPCE 610 620 630 640 650 660 TCGDAVCAFG AVCSAGQCVC PRCEHPPPGP VCGSDGVTYG SACELREAAC LQQTQIEEAR 670 680 690 700 710 720 AGPCEQAECG SGGSGSGEDG DCEQELCRQR GGIWDEDSED GPCVCDFSCQ SVPGSPVCGS 730 740 750 760 770 780 DGVTYSTECE LKKARCESQR GLYVAAQGAC RGPTFAPLPP VAPLHCAQTP YGCCQDNITA 790 800 810 820 830 840 ARGVGLAGCP SACQCNPHGS YGGTCDPATG QCSCRPGVGG LRCDRCEPGF WNFRGIVTDG 850 860 870 880 890 900 RSGCTPCSCD PQGAVRDDCE QMTGLCSCKP GVAGPKCGQC PDGRALGPAG CEADASAPAT 910 920 930 940 950 960 CAEMRCEFGA RCVEESGSAH CVCPMLTCPE ANATKVCGSD GVTYGNECQL KTIACRQGLQ 970 980 990 1000 1010 1020 ISIQSLGPCQ EAVAPSTHPT SASVTVTTPG LLLSQALPAP PGALPLAPSS TAHSQTTPPP 1030 1040 1050 1060 1070 1080 SSRPRTTASV PRTTVWPVLT VPPTAPSPAP SLVASAFGES GSTDGSSDEE LSGDQEASGG 1090 1100 1110 1120 1130 1140 GSGGLEPLEG SSVATPGPPV ERASCYNSAL GCCSDGKTPS LDAEGSNCPA TKVFQGVLEL 1150 1160 1170 1180 1190 1200 EGVEGQELFY TPEMADPKSE LFGETARSIE STLDDLFRNS DVKKDFRSVR LRDLGPGKSV 1210 1220 1230 1240 1250 1260 RAIVDVHFDP TTAFRAPDVA RALLRQIQVS RRRSLGVRRP LQEHVRFMDF DWFPAFITGA 1270 1280 1290 1300 1310 1320 TSGAIAAGAT ARATTASRLP SSAVTPRAPH PSHTSQPVAK TTAAPTTRRP PTTAPSRVPG 1330 1340 1350 1360 1370 1380 RRPPAPQQPP KPCDSQPCFH GGTCQDWALG GGFTCSCPAG RGGAVCEKVL GAPVPAFEGR 1390 1400 1410 1420 1430 1440 SFLAFPTLRA YHTLRLALEF RALEPQGLLL YNGNARGKDF LALALLDGRV QLRFDTGSGP 1450 1460 1470 1480 1490 1500 AVLTSAVPVE PGQWHRLELS RHWRRGTLSV DGETPVLGES PSGTDGLNLD TDLFVGGVPE 1510 1520 1530 1540 1550 1560 DQAAVALERT FVGAGLRGCI RLLDVNNQRL ELGIGPGAAT RGSGVGECGD HPCLPNPCHG 1570 1580 1590 1600 1610 1620 GAPCQNLEAG RFHCQCPPGR VGPTCADEKS PCQPNPCHGA APCRVLPEGG AQCECPLGRE 1630 1640 1650 1660 1670 1680 GTFCQTASGQ DGSGPFLADF NGFSHLELRG LHTFARDLGE KMALEVVFLA RGPSGLLLYN 1690 1700 1710 1720 1730 1740 GQKTDGKGDF VSLALRDRRL EFRYDLGKGA AVIRSREPVT LGAWTRVSLE RNGRKGALRV 1750 1760 1770 1780 1790 1800 GDGPRVLGES PVPHTVLNLK EPLYVGGAPD FSKLARAAAV SSGFDGAIQL VSLGGRQLLT 1810 1820 1830 1840 1850 1860 PEHVLRQVDV TSFAGHPCTR ASGHPCLNGA SCVPREAAYV CLCPGGFSGP HCEKGLVEKS 1870 1880 1890 1900 1910 1920 AGDVDTLAFD GRTFVEYLNA VTESELANEI PVPETLDSGA LHEKALQSNH FELSLRTEAT 1930 1940 1950 1960 1970 1980 QGLVLWSGKA TERADYVALA IVDGHLQLSY NLGSQPVVLR STVPVNTNRW LRVVAHREQR 1990 2000 2010 2020 2030 2040 EGSLQVGNEA PVTGSSPLGA TQLDTDGALW LGGLPELPVG PALPKAYGTG FVGCLRDVVV 2050 2060 GRHPLHLLED AVTKPELRPC PTP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Primary structure and high expression of human agrin in basement membranes of adult lung and kidney." Groffen A.J.A., Buskens C.A.F., Van Kuppevelt T.H., Veerkamp J.H., Monnens L.A.H., Van den Heuvel L.P.W.J. Eur. J. Biochem. 254:123-128(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), TISSUE SPECIFICITY.
[2]	Kato S. Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3). Tissue: Retinal pigment epithelium.
[3]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (ISOFORM 3), NUCLEOTIDE SEQEUNCE [LARGE SCALE GENOMIC DNA] OF 1703-1949 (ISOFORM 6).
[4]	"Agrin binds to the nerve-muscle basal lamina via laminin." Denzer A.J., Brandenberger R., Gesemann M., Chiquet M., Ruegg M.A. J. Cell Biol. 137:671-683(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 20-172, INTERACTION WITH LAMININ.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1558-2045. Tissue: Brain, Colon and Kidney.
[6]	"An alternative amino-terminus expressed in the central nervous system converts agrin to a type II transmembrane protein." Neumann F.R., Bittcher G., Annies M., Schumacher B., Kroger S., Ruegg M.A. Mol. Cell. Neurosci. 17:208-225(2001) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION OF TRANSMEMBRANE ISOFORM (ISOFORM 2).
[7]	"Identification of agrinSN isoform and muscle-specific receptor tyrosine kinase (MuSK) in sperm." Kumar P., Ferns M.J., Meizel S. Biochem. Biophys. Res. Commun. 342:522-528(2006) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION OF TRANSMEMBRANE ISOFORM (ISOFORM 2), ALTERNATIVE SPLICING, TISSUE SPECIFICITY.
[8]	Erratum Kumar P., Ferns M.J., Meizel S. Biochem. Biophys. Res. Commun. 344:453-453(2006)
[9]	"Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry." Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H. J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-135, MASS SPECTROMETRY. Tissue: Liver.
[10]	"Proteomics characterization of extracellular space components in the human aorta." Didangelos A., Yin X., Mandal K., Baumert M., Jahangiri M., Mayr M. Mol. Cell. Proteomics 9:2048-2062(2010) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
[11]	"Agrin binds to the N-terminal region of Lrp4 protein and stimulates association between Lrp4 and the first immunoglobulin-like domain in muscle-specific kinase (MuSK)." Zhang W., Coldefy A.S., Hubbard S.R., Burden S.J. J. Biol. Chem. 286:40624-40630(2011) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH LRP4, FUNCTION.
[12]	"C-terminal Agrin Fragment as a potential marker for sarcopenia caused by degeneration of the neuromuscular junction." Drey M., Sieber C.C., Bauer J.M., Uter W., Dahinden P., Fariello R.G., Vrijbloed J.W. Exp. Gerontol. 48:76-80(2013) [PubMed] [Europe PMC] [Abstract] Cited for: POTENTIAL USAGE AS A BIOMARKER FOR SARCOPENIA.
[13]	"Identification of an agrin mutation that causes congenital myasthenia and affects synapse function." Huze C., Bauche S., Richard P., Chevessier F., Goillot E., Gaudon K., Ben Ammar A., Chaboud A., Grosjean I., Lecuyer H.A., Bernard V., Rouche A., Alexandri N., Kuntzer T., Fardeau M., Fournier E., Brancaccio A., Ruegg M.A. , Koenig J., Eymard B., Schaeffer L., Hantai D. Am. J. Hum. Genet. 85:155-167(2009) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANT LGM ARG-1709, VARIANTS LEU-23; ASN-58; ILE-105; MET-267; SER-375; VAL-728; ARG-852; MET-984; PHE-1088; LYS-1118; ARG-1135; LEU-1240; ARG-1341; LEU-1451; THR-1514; HIS-1565; ILE-1666; GLN-1671; PRO-1698; HIS-1734; ASN-1789 AND VAL-2045, FUNCTION, CHARACTERIZATION OF VARIANT LGM ARG-1709.
[14]	Erratum Huze C., Bauche S., Richard P., Chevessier F., Goillot E., Gaudon K., Ben Ammar A., Chaboud A., Grosjean I., Lecuyer H.A., Bernard V., Rouche A., Alexandri N., Kuntzer T., Fardeau M., Fournier E., Brancaccio A., Ruegg M.A. , Koenig J., Eymard B., Schaeffer L., Hantai D. Am. J. Hum. Genet. 85:536-536(2009)
[15]	"LG2 agrin mutation causing severe congenital myasthenic syndrome mimics functional characteristics of non-neural (z-) agrin." Maselli R.A., Fernandez J.M., Arredondo J., Navarro C., Ngo M., Beeson D., Cagney O., Williams D.C., Wollmann R.L., Yarov-Yarovoy V., Ferns M.J. Hum. Genet. 131:1123-1135(2012) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANT LGM PHE-1727, INTERACTION WITH DAG1, CHARACTERIZATION OF PHE-1727.
+	Additional computationally mapped references.

Web resources

The Leiden Muscular Dystrophy pages, Agrin (AGRN)

Leiden Open Variation Database (LOVD)

Cross-references

Sequence databases
EMBL GenBank DDBJ	U84406 mRNA. Translation: AAB52917.1. AB191264 mRNA. Translation: BAD52440.1. AL645608 Genomic DNA. Translation: CAI15575.2. AL645608 Genomic DNA. Translation: CAI15576.1. AF016903 mRNA. Translation: AAC39776.1. BC004220 mRNA. Translation: AAH04220.2. BC007649 mRNA. Translation: AAH07649.1. BC034009 mRNA. Translation: AAH34009.1. BC063620 mRNA. Translation: AAH63620.1.
IPI	IPI00374563.
RefSeq	NP_940978.2. NM_198576.3.
UniGene	Hs.273330.
3D structure databases
ProteinModelPortal	O00468.
ModBase	Search...
Protein-protein interaction databases
IntAct	O00468. 5 interactions.
MINT	MINT-4053526.
STRING	9606.ENSP00000368678.
PTM databases
PhosphoSite	O00468.
Proteomic databases
PaxDb	O00468.
PRIDE	O00468.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000379370; ENSP00000368678; ENSG00000188157.
GeneID	375790.
KEGG	hsa:375790.
UCSC	uc001ack.2. human.
Organism-specific databases
CTD	375790.
GeneCards	GC01P000946.
HGNC	HGNC:329. AGRN.
HPA	HPA040090.
MIM	103320. gene. 254300. phenotype.
neXtProt	NX_O00468.
Orphanet	98913. Postsynaptic congenital myasthenic syndromes.
PharmGKB	PA24626.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG312635.
HOGENOM	HOG000033860.
HOVERGEN	HBG080471.
InParanoid	O00468.
KO	K06254.
OMA	TNRWLRV.
OrthoDB	EOG4HQDHD.
Enzyme and pathway databases
Reactome	REACT_111045. Developmental Biology. REACT_111217. Metabolism. REACT_116125. Disease.
Gene expression databases
ArrayExpress	O00468.
Bgee	O00468.
CleanEx	HS_AGRN.
Genevestigator	O00468.
GermOnline	ENSG00000188157. Homo sapiens.
Family and domain databases
Gene3D	2.60.120.200. 3 hits.
InterPro	IPR004850. Agrin_NtA. IPR008985. ConA-like_lec_gl_sf. IPR013320. ConA-like_subgrp. IPR000742. EG-like_dom. IPR013032. EGF-like_CS. IPR002049. EGF_laminin. IPR003645. Fol_N. IPR002350. Kazal_dom. IPR001791. Laminin_G. IPR000082. SEA. IPR008993. TIMP-like_OB-fold. [Graphical view]
Pfam	PF00008. EGF. 3 hits. PF00050. Kazal_1. 3 hits. PF07648. Kazal_2. 6 hits. PF00053. Laminin_EGF. 2 hits. PF00054. Laminin_G_1. 3 hits. PF03146. NtA. 1 hit. PF01390. SEA. 1 hit. [Graphical view]
SMART	SM00181. EGF. 4 hits. SM00180. EGF_Lam. 2 hits. SM00274. FOLN. 5 hits. SM00280. KAZAL. 9 hits. SM00282. LamG. 3 hits. SM00200. SEA. 1 hit. [Graphical view]
SUPFAM	SSF49899. ConA_like_lec_gl. 3 hits. SSF50242. TIMP_like. 1 hit.
PROSITE	PS00022. EGF_1. 6 hits. PS01186. EGF_2. 1 hit. PS50026. EGF_3. 4 hits. PS01248. EGF_LAM_1. 1 hit. PS50027. EGF_LAM_2. 2 hits. PS51465. KAZAL_2. 9 hits. PS50025. LAM_G_DOMAIN. 3 hits. PS51121. NTA. 1 hit. PS50024. SEA. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	375790.
NextBio	100617.
SOURCE	Search...

Entry information

Entry name

AGRIN_HUMAN

Accession

Primary (citable) accession number: O00468
Secondary accession number(s): Q5SVA1 , Q5SVA2, Q60FE1, Q7KYS8, Q8N4J5, Q96IC1, Q9BTD4

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2004
Last sequence update:	March 6, 2013
Last modified:	May 1, 2013
This is version 124 of the entry and version 5 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.