O00461 (GOLI4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Golgi integral membrane protein 4

Alternative name(s):
Golgi integral membrane protein, cis
Short name=GIMPc
Golgi phosphoprotein 4
Golgi-localized phosphoprotein of 130 kDa
Short name=Golgi phosphoprotein of 130 kDa

Gene names

Name:	GOLIM4
Synonyms:	GIMPC, GOLPH4, GPP130

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	696 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays a role in endosome to Golgi protein trafficking; mediates protein transport along the late endosome-bypass pathway from the early endosome to the Golgi. Ref.3
Subcellular location	Golgi apparatus › Golgi stack membrane; Single-pass type II membrane protein. Endosome membrane; Single-pass type II membrane protein. Note: Localizes to cis and medial Golgi cisternae. Probably cycles between early Golgi and distal compartments like endosome. Ref.1 Ref.2
Post-translational modification	Phosphorylated probably by c-AMP-dependent kinases in its lumenal part. Ref.1 Ref.4 O-glycosylated; modified by sialic acid residues By similarity. Ref.1 N-glycosylated; N-glycans are probably of the complex type and modified by sialic acid residues. Ref.1
Sequence similarities	Belongs to the GOLIM4 family.

Ontologies

Keywords
Biological process	Transport
Cellular component	Endosome Golgi apparatus Membrane
Coding sequence diversity	Polymorphism
Domain	Coiled coil Signal-anchor Transmembrane Transmembrane helix
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	Golgi cisterna membrane Inferred from electronic annotation. Source: UniProtKB-SubCell Golgi lumen Traceable author statement. Source: ProtInc cis-Golgi network Traceable author statement. Source: ProtInc endocytic vesicle Traceable author statement. Source: ProtInc endosome membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Traceable author statement. Source: ProtInc nucleus Inferred from direct assay. Source: HPA
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 696	696	Golgi integral membrane protein 4		PRO_0000285097
Regions
	Topological domain	1 – 12	12	Cytoplasmic Potential
	Transmembrane	13 – 33	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	34 – 696	663	Lumenal Potential
	Region	38 – 107	70	Golgi targeting
	Region	80 – 175	96	Endosome targeting
	Region	176 – 248	73	Golgi targeting
	Coiled coil	35 – 244	210	Potential
	Compositional bias	311 – 681	371	Glu-rich
	Compositional bias	404 – 513	110	Gln-rich
Amino acid modifications
	Modified residue	394	1	Phosphotyrosine Ref.4
	Modified residue	396	1	Phosphoserine Ref.4
	Modified residue	673	1	Phosphotyrosine By similarity
	Glycosylation	257	1	N-linked (GlcNAc...) Potential
Natural variations
	Natural variant	312	1	A → V in a breast cancer sample; somatic mutation. Ref.6		VAR_036611

Sequences

	Sequence		Length	Mass (Da)
	O00461 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: A3BA9AE3D52F7174	FASTA	696	81,880
	10 20 30 40 50 60 MGNGMCSRKQ KRIFQTLLLL TVVFGFLYGA MLYYELQTQL RKAEAVALKY QQHQESLSAQ 70 80 90 100 110 120 LQVVYEHRSR LEKSLQKERL EHKKAKEDFL VYKLEAQETL NKGRQDSNSR YSALNVQHQM 130 140 150 160 170 180 LKSQHEELKK QHSDLEEEHR KQGEDFSRTF NDHKQKYLQL QQEKEQELSK LKETVYNLRE 190 200 210 220 230 240 ENRQLRKAHQ DIHTQLQDVK QQHKNLLSEH EQLVVTLEDH KSALAAAQTQ VAEYKQLKDT 250 260 270 280 290 300 LNRIPSLRKP DPAEQQNVTQ VAHSPQGYNT AREKPTREVQ EVSRNNDVWQ NHEAVPGRAE 310 320 330 340 350 360 DTKLYAPTHK EAEFQAPPEP IQQEVERREP EEHQVEEEHR KALEEEEMEQ VGQAEHLEEE 370 380 390 400 410 420 HDPSPEEQDR EWKEQHEQRE AANLLEGHAR AEVYPSAKPM IKFQSPYEEQ LEQQRLAVQQ 430 440 450 460 470 480 VEEAQQLREH QEALHQQRLQ GHLLRQQEQQ QQQVAREMAL QRQAELEEGR PQHQEQLRQQ 490 500 510 520 530 540 AHYDAMDNDI VQGAEDQGIQ GEEGAYERDN QHQDEAEGDP GNRHEPREQG PREADPESEA 550 560 570 580 590 600 DRAAVEDINP ADDPNNQGED EFEEAEQVRE ENLPDENEEQ KQSNQKQENT EVEEHLVMAG 610 620 630 640 650 660 NPDQQEDNVD EQYQEEAEEE VQEDLTEEKK RELEHNAEET YGENDENTDD KNNDGEEQEV 670 680 690 RDDNRPKGRE EHYEEEEEEE EDGAAVAEKS HRRAEM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and overexpression of GPP130/GIMPc: evidence for saturable pH-sensitive targeting of a type II early Golgi membrane protein." Linstedt A.D., Mehta A., Suhan J., Reggio H., Hauri H.-P. Mol. Biol. Cell 8:1073-1087(1997) [PubMed: 9201717] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, PHOSPHORYLATION, GLYCOSYLATION, TOPOLOGY. Tissue: Small intestine.
[2]	"Lumenal endosomal and Golgi-retrieval determinants involved in pH-sensitive targeting of an early Golgi protein." Bachert C., Lee T.H., Linstedt A.D. Mol. Biol. Cell 12:3152-3160(2001) [PubMed: 11598199] [Abstract] Cited for: SUBCELLULAR LOCATION.
[3]	"A cycling cis-Golgi protein mediates endosome-to-Golgi traffic." Natarajan R., Linstedt A.D. Mol. Biol. Cell 15:4798-4806(2004) [PubMed: 15331763] [Abstract] Cited for: FUNCTION.
[4]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-394 AND SER-396, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[5]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[6]	"The consensus coding sequences of human breast and colorectal cancers." Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E. Science 314:268-274(2006) [PubMed: 16959974] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] VAL-312.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U55853 mRNA. Translation: AAB58419.1.
IPI	IPI00004962.
RefSeq	NP_055313.1. NM_014498.3.
UniGene	Hs.143600.
3D structure databases
ProteinModelPortal	O00461.
ModBase	Search...
Protein-protein interaction databases
STRING	O00461.
PTM databases
PhosphoSite	O00461.
Proteomic databases
PeptideAtlas	O00461.
PRIDE	O00461.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000470487; ENSP00000417354; ENSG00000173905.
GeneID	27333.
KEGG	hsa:27333.
UCSC	uc003ffe.2. human.
Organism-specific databases
CTD	27333.
GeneCards	GC03M167727.
H-InvDB	HIX0030877.
HGNC	HGNC:15448. GOLIM4.
HPA	HPA001677. HPA002315.
MIM	606805. gene.
neXtProt	NX_O00461.
PharmGKB	PA162390001.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG17739.
GeneTree	ENSGT00390000004096.
HOGENOM	HBG444834.
HOVERGEN	HBG107959.
InParanoid	O00461.
OMA	WKEQREQ.
OrthoDB	EOG4RBQJB.
PhylomeDB	O00461.
Gene expression databases
ArrayExpress	O00461.
Bgee	O00461.
CleanEx	HS_GOLIM4.
Genevestigator	O00461.
Family and domain databases
ProtoNet	Search...
Other
NextBio	50382.
SOURCE	Search...

Entry information

Entry name

GOLI4_HUMAN

Accession

Primary (citable) accession number: O00461

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 2007
Last sequence update:	July 1, 1997
Last modified:	January 25, 2012
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.