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O00461 (GOLI4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Golgi integral membrane protein 4
Alternative name(s):
Golgi integral membrane protein, cis
Short name=GIMPc
Golgi phosphoprotein 4
Golgi-localized phosphoprotein of 130 kDa
Short name=Golgi phosphoprotein of 130 kDa
Gene names
Name:GOLIM4
Synonyms:GIMPC, GOLPH4, GPP130
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length696 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Plays a role in endosome to Golgi protein trafficking; mediates protein transport along the late endosome-bypass pathway from the early endosome to the Golgi. Ref.3

Subcellular location

Golgi apparatusGolgi stack membrane; Single-pass type II membrane protein. Endosome membrane; Single-pass type II membrane protein. Note: Localizes to cis and medial Golgi cisternae. Probably cycles between early Golgi and distal compartments like endosome. Ref.1 Ref.2

Post-translational modification

Phosphorylated probably by c-AMP-dependent kinases in its lumenal part. Ref.1

O-glycosylated; modified by sialic acid residues By similarity. Ref.1

N-glycosylated; N-glycans are probably of the complex type and modified by sialic acid residues. Ref.1

Sequence similarities

Belongs to the GOLIM4 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 696696Golgi integral membrane protein 4
PRO_0000285097

Regions

Topological domain1 – 1212Cytoplasmic Potential
Transmembrane13 – 3321Helical; Signal-anchor for type II membrane protein; Potential
Topological domain34 – 696663Lumenal Potential
Region38 – 10770Golgi targeting
Region80 – 17596Endosome targeting
Region176 – 24873Golgi targeting
Coiled coil35 – 244210 Potential
Compositional bias311 – 681371Glu-rich
Compositional bias404 – 513110Gln-rich

Amino acid modifications

Modified residue6731Phosphotyrosine By similarity
Glycosylation2571N-linked (GlcNAc...) Potential

Natural variations

Natural variant3121A → V in a breast cancer sample; somatic mutation. Ref.6
VAR_036611

Sequences

Sequence LengthMass (Da)Tools
O00461 [UniParc].

Last modified July 1, 1997. Version 1.
Checksum: A3BA9AE3D52F7174

FASTA69681,880
        10         20         30         40         50         60 
MGNGMCSRKQ KRIFQTLLLL TVVFGFLYGA MLYYELQTQL RKAEAVALKY QQHQESLSAQ 

        70         80         90        100        110        120 
LQVVYEHRSR LEKSLQKERL EHKKAKEDFL VYKLEAQETL NKGRQDSNSR YSALNVQHQM 

       130        140        150        160        170        180 
LKSQHEELKK QHSDLEEEHR KQGEDFSRTF NDHKQKYLQL QQEKEQELSK LKETVYNLRE 

       190        200        210        220        230        240 
ENRQLRKAHQ DIHTQLQDVK QQHKNLLSEH EQLVVTLEDH KSALAAAQTQ VAEYKQLKDT 

       250        260        270        280        290        300 
LNRIPSLRKP DPAEQQNVTQ VAHSPQGYNT AREKPTREVQ EVSRNNDVWQ NHEAVPGRAE 

       310        320        330        340        350        360 
DTKLYAPTHK EAEFQAPPEP IQQEVERREP EEHQVEEEHR KALEEEEMEQ VGQAEHLEEE 

       370        380        390        400        410        420 
HDPSPEEQDR EWKEQHEQRE AANLLEGHAR AEVYPSAKPM IKFQSPYEEQ LEQQRLAVQQ 

       430        440        450        460        470        480 
VEEAQQLREH QEALHQQRLQ GHLLRQQEQQ QQQVAREMAL QRQAELEEGR PQHQEQLRQQ 

       490        500        510        520        530        540 
AHYDAMDNDI VQGAEDQGIQ GEEGAYERDN QHQDEAEGDP GNRHEPREQG PREADPESEA 

       550        560        570        580        590        600 
DRAAVEDINP ADDPNNQGED EFEEAEQVRE ENLPDENEEQ KQSNQKQENT EVEEHLVMAG 

       610        620        630        640        650        660 
NPDQQEDNVD EQYQEEAEEE VQEDLTEEKK RELEHNAEET YGENDENTDD KNNDGEEQEV 

       670        680        690 
RDDNRPKGRE EHYEEEEEEE EDGAAVAEKS HRRAEM 

« Hide

References

« Hide 'large scale' references
[1]"Sequence and overexpression of GPP130/GIMPc: evidence for saturable pH-sensitive targeting of a type II early Golgi membrane protein."
Linstedt A.D., Mehta A., Suhan J., Reggio H., Hauri H.-P.
Mol. Biol. Cell 8:1073-1087(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, PHOSPHORYLATION, GLYCOSYLATION, TOPOLOGY.
Tissue: Small intestine.
[2]"Lumenal endosomal and Golgi-retrieval determinants involved in pH-sensitive targeting of an early Golgi protein."
Bachert C., Lee T.H., Linstedt A.D.
Mol. Biol. Cell 12:3152-3160(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[3]"A cycling cis-Golgi protein mediates endosome-to-Golgi traffic."
Natarajan R., Linstedt A.D.
Mol. Biol. Cell 15:4798-4806(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[4]"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[5]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[6]"The consensus coding sequences of human breast and colorectal cancers."
Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. expand/collapse author list , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E.
Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANT [LARGE SCALE ANALYSIS] VAL-312.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U55853 mRNA. Translation: AAB58419.1.
RefSeqNP_055313.1. NM_014498.3.
UniGeneHs.143600.

3D structure databases

ProteinModelPortalO00461.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid118146. 3 interactions.
MINTMINT-5002147.
STRING9606.ENSP00000417354.

PTM databases

PhosphoSiteO00461.

Proteomic databases

PaxDbO00461.
PeptideAtlasO00461.
PRIDEO00461.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000470487; ENSP00000417354; ENSG00000173905.
GeneID27333.
KEGGhsa:27333.
UCSCuc003ffe.2. human.

Organism-specific databases

CTD27333.
GeneCardsGC03M167727.
HGNCHGNC:15448. GOLIM4.
HPAHPA001677.
HPA002315.
MIM606805. gene.
neXtProtNX_O00461.
PharmGKBPA162390001.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG122835.
HOGENOMHOG000112755.
HOVERGENHBG107959.
InParanoidO00461.
OMAREWKEQH.
OrthoDBEOG7G4QDS.
PhylomeDBO00461.
TreeFamTF333101.

Gene expression databases

ArrayExpressO00461.
BgeeO00461.
CleanExHS_GOLIM4.
GenevestigatorO00461.

Family and domain databases

ProtoNetSearch...

Other

ChiTaRSGOLIM4. human.
GenomeRNAi27333.
NextBio50382.
PROO00461.
SOURCESearch...

Entry information

Entry nameGOLI4_HUMAN
AccessionPrimary (citable) accession number: O00461
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: July 1, 1997
Last modified: April 16, 2014
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 3

Human chromosome 3: entries, gene names and cross-references to MIM