Reviewed, UniProtKB/Swiss-Prot O00459 (P85B_HUMAN)

Names and origin

Protein names

Recommended name:
    Phosphatidylinositol 3-kinase regulatory subunit beta
      Short name=PtdIns-3-kinase regulatory subunit beta
      Short name=PI3-kinase regulatory subunit beta
      Short name=PI3K regulatory subunit beta
Alternative name(s):
    Phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta
      Short name=PtdIns-3-kinase regulatory subunit p85-beta
      Short name=PI3-kinase subunit p85-beta

Gene names

Name:

PIK3R2

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	728 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Binds to activated (phosphorylated) protein-tyrosine kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane.
Subunit structure	Heterodimer of a p110 (catalytic) and a p85 (regulatory) subunits.
Sequence similarities	Belongs to the PI3K p85 subunit family. Contains 1 Rho-GAP domain. Contains 2 SH2 domains. Contains 1 SH3 domain.

Ontologies

Keywords
Coding sequence diversity	Polymorphism
Domain	Repeat SH2 domain SH3 domain
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	negative regulation of anti-apoptosis Inferred from sequence or structural similarity. Source: UniProtKB signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	phosphoinositide 3-kinase complex Inferred from electronic annotation. Source: InterPro
Molecular function	phosphoinositide 3-kinase regulator activity Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
HCK	P08631	1	EBI-346930,EBI-346340

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 728	728	Phosphatidylinositol 3-kinase regulatory subunit beta		PRO_0000080763
Regions
	Domain	4 – 80	77	SH3
	Domain	109 – 295	187	Rho-GAP
	Domain	330 – 425	96	SH2 1
	Domain	622 – 716	95	SH2 2
Amino acid modifications
	Modified residue	262	1	Phosphoserine
	Modified residue	263	1	Phosphoserine
	Modified residue	365	1	Phosphotyrosine Ref.7
	Modified residue	464	1	Phosphotyrosine Ref.7 Ref.5 Ref.6 Ref.9
	Modified residue	467	1	Phosphotyrosine Ref.7
	Modified residue	605	1	Phosphotyrosine Ref.11
Natural variations
	Natural variant	234	1	R → S: dbSNP rs2241088. Ref.3		VAR_030679
	Natural variant	313	1	P → S: dbSNP rs1011320. Ref.3		VAR_030680

Sequences

	Sequence		Length	Mass (Da)
	O00459-1 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: 80C2AF244977346B	FASTA	728	81,624
	10 20 30 40 50 60 MAGPEGFQYR ALYPFRRERP EDLELLPGDV LVVSRAALQA LGVAEGGERC PQSVGWMPGL 70 80 90 100 110 120 NERTRQRGDF PGTYVEFLGP VALARPGPRP RGPRPLPARP RDGAPEPGLT LPDLPEQFSP 130 140 150 160 170 180 PDVAPPLLVK LVEAIERTGL DSESHYRPEL PAPRTDWSLS DVDQWDTAAL ADGIKSFLLA 190 200 210 220 230 240 LPAPLVTPEA SAEARRALRE AAGPVGPALE PPTLPLHRAL TLRFLLQHLG RVARRAPALG 250 260 270 280 290 300 PAVRALGATF GPLLLRAPPP PSSPPPGGAP DGSEPSPDFP ALLVEKLLQE HLEEQEVAPP 310 320 330 340 350 360 ALPPKPPKAK PAPTVLANGG SPPSLQDAEW YWGDISREEV NEKLRDTPDG TFLVRDASSK 370 380 390 400 410 420 IQGEYTLTLR KGGNNKLIKV FHRDGHYGFS EPLTFCSVVD LINHYRHESL AQYNAKLDTR 430 440 450 460 470 480 LLYPVSKYQQ DQIVKEDSVE AVGAQLKVYH QQYQDKSREY DQLYEEYTRT SQELQMKRTA 490 500 510 520 530 540 IEAFNETIKI FEEQGQTQEK CSKEYLERFR REGNEKEMQR ILLNSERLKS RIAEIHESRT 550 560 570 580 590 600 KLEQQLRAQA SDNREIDKRM NSLKPDLMQL RKIRDQYLVW LTQKGARQKK INEWLGIKNE 610 620 630 640 650 660 TEDQYALMED EDDLPHHEER TWYVGKINRT QAEEMLSGKR DGTFLIRESS QRGCYACSVV 670 680 690 700 710 720 VDGDTKHCVI YRTATGFGFA EPYNLYGSLK ELVLHYQHAS LVQHNDALTV TLAHPVRAPG PGPPPAAR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"An oncogenic fusion product of the phosphatidylinositol 3-kinase p85beta subunit and HUMORF8, a putative deubiquitinating enzyme." Janssen J.W.G., Schleithhoff L., Bartram C.R., Schulz A.S. Oncogene 16:1767-1772(1998) [PubMed: 9582025] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Chromosomal localization of human p85 alpha, a subunit of phosphatidylinositol 3-kinase, and its homologue p85 beta." Volinia S., Patracchini P., Otsu M., Hiles I., Gout I., Calzolari E., Bernardi F., Rooke L., Waterfield M.D. Oncogene 7:789-793(1992) [PubMed: 1314371] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The DNA sequence and biology of human chromosome 19." Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M. Nature 428:529-535(2004) [PubMed: 15057824] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANTS SER-234 AND SER-313.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain and Placenta.
[5]	"Time-resolved mass spectrometry of tyrosine phosphorylation sites in the epidermal growth factor receptor signaling network reveals dynamic modules." Zhang Y., Wolf-Yadlin A., Ross P.L., Pappin D.J., Rush J., Lauffenburger D.A., White F.M. Mol. Cell. Proteomics 4:1240-1250(2005) [PubMed: 15951569] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-464, MASS SPECTROMETRY. Tissue: Epithelium.
[6]	"Immunoaffinity profiling of tyrosine phosphorylation in cancer cells." Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., Zhang H., Zha X.-M., Polakiewicz R.D., Comb M.J. Nat. Biotechnol. 23:94-101(2005) [PubMed: 15592455] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-464, MASS SPECTROMETRY.
[7]	"Global survey of phosphotyrosine signaling identifies oncogenic kinases in lung cancer." Rikova K., Guo A., Zeng Q., Possemato A., Yu J., Haack H., Nardone J., Lee K., Reeves C., Li Y., Hu Y., Tan Z., Stokes M., Sullivan L., Mitchell J., Wetzel R., Macneill J., Ren J.M. , Yuan J., Bakalarski C.E., Villen J., Kornhauser J.M., Smith B., Li D., Zhou X., Gygi S.P., Gu T.-L., Polakiewicz R.D., Rush J., Comb M.J. Cell 131:1190-1203(2007) [PubMed: 18083107] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-365; TYR-464 AND TYR-467, MASS SPECTROMETRY.
[8]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[9]	"An extensive survey of tyrosine phosphorylation revealing new sites in human mammary epithelial cells." Heibeck T.H., Ding S.-J., Opresko L.K., Zhao R., Schepmoes A.A., Yang F., Tolmachev A.V., Monroe M.E., Camp D.G. II, Smith R.D., Wiley H.S., Qian W.-J. J. Proteome Res. 8:3852-3861(2009) [PubMed: 19534553] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-464, MASS SPECTROMETRY. Tissue: Mammary epithelium.
[10]	"Large-scale proteomics analysis of the human kinome." Oppermann F.S., Gnad F., Olsen J.V., Hornberger R., Greff Z., Keri G., Mann M., Daub H. Mol. Cell. Proteomics 8:1751-1764(2009) [PubMed: 19369195] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-262 AND SER-263, MASS SPECTROMETRY.
[11]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed: 19690332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-605, MASS SPECTROMETRY. Tissue: T-cell.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X80907 mRNA. Translation: CAA56868.1. AC007192 Genomic DNA. Translation: AAD22671.1. BC070082 mRNA. Translation: AAH70082.1. BC090249 mRNA. Translation: AAH90249.1.
IPI	IPI00011736.
PIR	H59435.
RefSeq	NP_005018.1.
UniGene	Hs.371344
3D structure databases
SMR	O00459. Positions 7-76, 107-258, 324-436, 428-596, 612-718.
ModBase	Search...
Protein-protein interaction databases
IntAct	O00459. 3 interactions.
STRING	O00459.
PTM databases
PhosphoSite	O00459.
Proteomic databases
PRIDE	O00459.
Genome annotation databases
Ensembl	ENST00000222254; ENSP00000222254; ENSG00000105647; Homo sapiens. [Genome view]
GeneID	5296.
KEGG	hsa:5296.
UCSC	uc002nia.1. human.
Organism-specific databases
CTD	5296.
GeneCards	GC19P018089.
H-InvDB	HIX0014905.
HGNC	HGNC:8980. PIK3R2.
MIM	603157. gene.
PharmGKB	PA33313.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG15816.
HOGENOM	HBG506175.
HOVERGEN	O00459.
InParanoid	O00459.
Enzyme and pathway databases
BioCyc	MetaCyc:ENSG00000105647-MONOMER.
Pathway_Interaction_DB	pi3kcipathway. Class I PI3K signaling events. trail_pathway. TRAIL signaling pathway.
Reactome	REACT_11044. Signaling by Rho GTPases. REACT_11061. Signalling by NGF. REACT_16888. Signaling by PDGF. REACT_498. Signaling by Insulin receptor. REACT_604. Hemostasis. REACT_6900. Signaling in Immune system.
Gene expression databases
ArrayExpress	O00459.
Bgee	O00459.
CleanEx	HS_PIK3R2.
Genevestigator	O00459.
GermOnline	ENSG00000105647. Homo sapiens.
Family and domain databases
InterPro	IPR001720. PI3kinase_P85. IPR008936. Rho_GTPase_activation_prot. IPR000198. RhoGAP. IPR000980. SH2. IPR001452. SH3_domain. [Graphical view]
Gene3D	G3DSA:1.10.555.10. RhoGAP. 1 hit. G3DSA:3.30.505.10. SH2. 2 hits.
PANTHER	PTHR10155. PI3kinase_P85. 1 hit.
Pfam	PF00620. RhoGAP. 1 hit. PF00017. SH2. 2 hits. [Graphical view]
PRINTS	PR00678. PI3KINASEP85. PR00401. SH2DOMAIN.
SMART	SM00324. RhoGAP. 1 hit. SM00252. SH2. 2 hits. SM00326. SH3. 1 hit. [Graphical view]
PROSITE	PS50238. RHOGAP. 1 hit. PS50001. SH2. 2 hits. PS50002. SH3. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	20470.
SOURCE	Search...

Entry information

Entry name

P85B_HUMAN

Accession

Primary (citable) accession number: O00459
Secondary accession number(s): Q5EAT5, Q9UPH9

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	July 1, 1997
Last modified:	February 9, 2010
This is version 103 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.