Reviewed,
UniProtKB/Swiss-Prot O00459 (P85B_HUMAN)
Last modified
February 9, 2010.
Version 103.
History...
Clusters with 100%,
90%,
50% identity |
Documents (5) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Phosphatidylinositol 3-kinase regulatory subunit beta Short name=PtdIns-3-kinase regulatory subunit beta Short name=PI3-kinase regulatory subunit beta Short name=PI3K regulatory subunit beta Alternative name(s): Phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta Short name=PtdIns-3-kinase regulatory subunit p85-beta Short name=PI3-kinase subunit p85-beta | ||
| Gene names |
| ||
| Organism | Homo sapiens (Human) [Complete proteome] | ||
| Taxonomic identifier | 9606 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 728 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Binds to activated (phosphorylated) protein-tyrosine kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. |
| Subunit structure | Heterodimer of a p110 (catalytic) and a p85 (regulatory) subunits. |
| Sequence similarities | Belongs to the PI3K p85 subunit family. Contains 1 Rho-GAP domain. Contains 2 SH2 domains. Contains 1 SH3 domain. |
Ontologies
| Keywords | |
|---|---|
| Coding sequence diversity | Polymorphism |
| Domain | Repeat SH2 domain SH3 domain |
| PTM | Phosphoprotein |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | negative regulation of anti-apoptosis Inferred from sequence or structural similarity. Source: UniProtKB signal transductionInferred from electronic annotation. Source: InterPro |
| Cellular component | phosphoinositide 3-kinase complex Inferred from electronic annotation. Source: InterPro |
| Molecular function | phosphoinositide 3-kinase regulator activity Inferred from electronic annotation. Source: InterPro protein bindingInferred from physical interaction. Source: IntAct |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 728 | 728 | Phosphatidylinositol 3-kinase regulatory subunit beta | PRO_0000080763 | |||||
Regions | |||||||||
| Domain | 4 – 80 | 77 | SH3 | ||||||
| Domain | 109 – 295 | 187 | Rho-GAP | ||||||
| Domain | 330 – 425 | 96 | SH2 1 | ||||||
| Domain | 622 – 716 | 95 | SH2 2 | ||||||
Amino acid modifications | |||||||||
| Modified residue | 262 | 1 | Phosphoserine | ||||||
| Modified residue | 263 | 1 | Phosphoserine | ||||||
| Modified residue | 365 | 1 | Phosphotyrosine Ref.7 | ||||||
| Modified residue | 464 | 1 | Phosphotyrosine Ref.7 Ref.5 Ref.6 Ref.9 | ||||||
| Modified residue | 467 | 1 | Phosphotyrosine Ref.7 | ||||||
| Modified residue | 605 | 1 | Phosphotyrosine Ref.11 | ||||||
Natural variations | |||||||||
| Natural variant | 234 | 1 | R → S: dbSNP rs2241088. Ref.3 | VAR_030679 | |||||
| Natural variant | 313 | 1 | P → S: dbSNP rs1011320. Ref.3 | VAR_030680 | |||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "An oncogenic fusion product of the phosphatidylinositol 3-kinase p85beta subunit and HUMORF8, a putative deubiquitinating enzyme." Janssen J.W.G., Schleithhoff L., Bartram C.R., Schulz A.S. Oncogene 16:1767-1772(1998) [PubMed: 9582025] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
| [2] | "Chromosomal localization of human p85 alpha, a subunit of phosphatidylinositol 3-kinase, and its homologue p85 beta." Volinia S., Patracchini P., Otsu M., Hiles I., Gout I., Calzolari E., Bernardi F., Rooke L., Waterfield M.D. Oncogene 7:789-793(1992) [PubMed: 1314371] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [3] | "The DNA sequence and biology of human chromosome 19." Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. Lucas S.M.Nature 428:529-535(2004) [PubMed: 15057824] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANTS SER-234 AND SER-313. |
| [4] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain and Placenta. |
| [5] | "Time-resolved mass spectrometry of tyrosine phosphorylation sites in the epidermal growth factor receptor signaling network reveals dynamic modules." Zhang Y., Wolf-Yadlin A., Ross P.L., Pappin D.J., Rush J., Lauffenburger D.A., White F.M. Mol. Cell. Proteomics 4:1240-1250(2005) [PubMed: 15951569] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-464, MASS SPECTROMETRY. Tissue: Epithelium. |
| [6] | "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells." Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., Zhang H., Zha X.-M., Polakiewicz R.D., Comb M.J. Nat. Biotechnol. 23:94-101(2005) [PubMed: 15592455] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-464, MASS SPECTROMETRY. |
| [7] | "Global survey of phosphotyrosine signaling identifies oncogenic kinases in lung cancer." Rikova K., Guo A., Zeng Q., Possemato A., Yu J., Haack H., Nardone J., Lee K., Reeves C., Li Y., Hu Y., Tan Z., Stokes M., Sullivan L., Mitchell J., Wetzel R., Macneill J., Ren J.M. Comb M.J.Cell 131:1190-1203(2007) [PubMed: 18083107] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-365; TYR-464 AND TYR-467, MASS SPECTROMETRY. |
| [8] | Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. |
| [9] | "An extensive survey of tyrosine phosphorylation revealing new sites in human mammary epithelial cells." Heibeck T.H., Ding S.-J., Opresko L.K., Zhao R., Schepmoes A.A., Yang F., Tolmachev A.V., Monroe M.E., Camp D.G. II, Smith R.D., Wiley H.S., Qian W.-J. J. Proteome Res. 8:3852-3861(2009) [PubMed: 19534553] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-464, MASS SPECTROMETRY. Tissue: Mammary epithelium. |
| [10] | "Large-scale proteomics analysis of the human kinome." Oppermann F.S., Gnad F., Olsen J.V., Hornberger R., Greff Z., Keri G., Mann M., Daub H. Mol. Cell. Proteomics 8:1751-1764(2009) [PubMed: 19369195] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-262 AND SER-263, MASS SPECTROMETRY. |
| [11] | "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed: 19690332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-605, MASS SPECTROMETRY. Tissue: T-cell. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | X80907 mRNA. Translation: CAA56868.1. AC007192 Genomic DNA. Translation: AAD22671.1. BC070082 mRNA. Translation: AAH70082.1. BC090249 mRNA. Translation: AAH90249.1. |
| IPI | IPI00011736. |
| PIR | H59435. |
| RefSeq | NP_005018.1. |
| UniGene | Hs.371344 |
3D structure databases | |
| SMR | O00459. Positions 7-76, 107-258, 324-436, 428-596, 612-718. |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | O00459. 3 interactions. |
| STRING | O00459. |
PTM databases | |
| PhosphoSite | O00459. |
Proteomic databases | |
| PRIDE | O00459. |
Genome annotation databases | |
| Ensembl | ENST00000222254; ENSP00000222254; ENSG00000105647; Homo sapiens. [Genome view] |
| GeneID | 5296. |
| KEGG | hsa:5296. |
| UCSC | uc002nia.1. human. |
Organism-specific databases | |
| CTD | 5296. |
| GeneCards | GC19P018089. |
| H-InvDB | HIX0014905. |
| HGNC | HGNC:8980. PIK3R2. |
| MIM | 603157. gene. |
| PharmGKB | PA33313. |
| GenAtlas | Search... |
Phylogenomic databases | |
| eggNOG | prNOG15816. |
| HOGENOM | HBG506175. |
| HOVERGEN | O00459. |
| InParanoid | O00459. |
Enzyme and pathway databases | |
| BioCyc | MetaCyc:ENSG00000105647-MONOMER. |
| Pathway_Interaction_DB | pi3kcipathway. Class I PI3K signaling events. trail_pathway. TRAIL signaling pathway. |
| Reactome | REACT_11044. Signaling by Rho GTPases. REACT_11061. Signalling by NGF. REACT_16888. Signaling by PDGF. REACT_498. Signaling by Insulin receptor. REACT_604. Hemostasis. REACT_6900. Signaling in Immune system. |
Gene expression databases | |
| ArrayExpress | O00459. |
| Bgee | O00459. |
| CleanEx | HS_PIK3R2. |
| Genevestigator | O00459. |
| GermOnline | ENSG00000105647. Homo sapiens. |
Family and domain databases | |
| InterPro | IPR001720. PI3kinase_P85. IPR008936. Rho_GTPase_activation_prot. IPR000198. RhoGAP. IPR000980. SH2. IPR001452. SH3_domain. [Graphical view] |
| Gene3D | G3DSA:1.10.555.10. RhoGAP. 1 hit. G3DSA:3.30.505.10. SH2. 2 hits. |
| PANTHER | PTHR10155. PI3kinase_P85. 1 hit. |
| Pfam | PF00620. RhoGAP. 1 hit. PF00017. SH2. 2 hits. [Graphical view] |
| PRINTS | PR00678. PI3KINASEP85. PR00401. SH2DOMAIN. |
| SMART | SM00324. RhoGAP. 1 hit. SM00252. SH2. 2 hits. SM00326. SH3. 1 hit. [Graphical view] |
| PROSITE | PS50238. RHOGAP. 1 hit. PS50001. SH2. 2 hits. PS50002. SH3. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 20470. |
| SOURCE | Search... |
Entry information
| Entry name | P85B_HUMAN | ||||||||
| Accession | Primary (citable) accession number: O00459 Secondary accession number(s): Q5EAT5, Q9UPH9 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
| Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. | ||||||||
Relevant documents
| Human chromosome 19 Human chromosome 19: entries, gene names and cross-references to MIM |
| Human entries with polymorphisms or disease mutations List of human entries with polymorphisms or disease mutations |
| Human polymorphisms and disease mutations Index of human polymorphisms and disease mutations |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |

Clusters with


