O00445 (SYT5_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Synaptotagmin-5

Alternative name(s):
Synaptotagmin V
Short name=SytV

Gene names

Name:

SYT5

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	386 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May be involved in Ca2+-dependent exocytosis of secretory vesicles through Ca2+ and phospholipid binding to the C2 domain or may serve as Ca2+ sensors in the process of vesicular trafficking and exocytosis. Regulates the Ca2+-dependent secretion of norepinephrine in PC12 cells. Required for export from the endocytic recycling compartment to the cell surface By similarity.
Cofactor	Binds 3 calcium ions per subunit. The ions are bound to the C2 domains By similarity.
Subunit structure	Homodimer. Can also form heterodimers with SYT6. Interacts with both alpha- and beta-tubulin By similarity.
Subcellular location	Cytoplasmic vesicle › secretory vesicle › synaptic vesicle membrane; Single-pass membrane protein By similarity. Recycling endosome membrane; Single-pass membrane protein By similarity. Note: In mast cells, localizes to the endocytic recycling compartment By similarity.
Sequence similarities	Belongs to the synaptotagmin family. Contains 2 C2 domains.

Ontologies

Keywords
Cellular component	Cell junction Cytoplasmic vesicle Endosome Membrane Synapse
Coding sequence diversity	Polymorphism
Domain	Repeat Transmembrane Transmembrane helix
Ligand	Calcium Metal-binding
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	energy reserve metabolic process Traceable author statement. Source: Reactome regulation of insulin secretion Traceable author statement. Source: Reactome small molecule metabolic process Traceable author statement. Source: Reactome synaptic transmission Traceable author statement Ref.1. Source: ProtInc
Cellular_component	cell junction Inferred from electronic annotation. Source: UniProtKB-KW dense core granule Inferred from electronic annotation. Source: Compara integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW neuron projection Inferred from electronic annotation. Source: Compara neuronal cell body Inferred from electronic annotation. Source: Compara perinuclear region of cytoplasm Inferred from electronic annotation. Source: Compara recycling endosome membrane Inferred from electronic annotation. Source: UniProtKB-SubCell synaptic vesicle membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	calcium-dependent phospholipid binding Inferred from electronic annotation. Source: Compara metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 386	386	Synaptotagmin-5		PRO_0000183951
Regions
	Topological domain	1 – 24	24	Vesicular Potential
	Transmembrane	25 – 45	21	Helical; Potential
	Topological domain	46 – 386	341	Cytoplasmic Potential
	Domain	124 – 211	88	C2 1
	Domain	253 – 344	92	C2 2
Sites
	Metal binding	138	1	Calcium 2; via carbonyl oxygen By similarity
	Metal binding	139	1	Calcium 1 By similarity
	Metal binding	139	1	Calcium 2 By similarity
	Metal binding	145	1	Calcium 1 By similarity
	Metal binding	197	1	Calcium 1 By similarity
	Metal binding	197	1	Calcium 2 By similarity
	Metal binding	198	1	Calcium 1; via carbonyl oxygen By similarity
	Metal binding	199	1	Calcium 1 By similarity
	Metal binding	199	1	Calcium 2 By similarity
	Metal binding	199	1	Calcium 3 By similarity
	Metal binding	202	1	Calcium 3 By similarity
	Metal binding	205	1	Calcium 2 By similarity
	Metal binding	205	1	Calcium 3 By similarity
Amino acid modifications
	Modified residue	278	1	Phosphotyrosine By similarity
Natural variations
	Natural variant	4	1	E → D. Corresponds to variant rs2301279 [ dbSNP | Ensembl ].		VAR_052240
	Natural variant	111	1	R → Q. Ref.4 Corresponds to variant rs11542503 [ dbSNP | Ensembl ].		VAR_034528
Experimental info
	Sequence conflict	24	1	H → R in BAG54160. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	O00445 [UniParc]. Last modified July 11, 2003. Version 2. Checksum: 96A36792D177FD55	FASTA	386	42,900
	10 20 30 40 50 60 MFPEPPTPGP PSPDTPPDSS RISHGPVPPW ALATIVLVSG LLIFSCCFCL YRKSCRRRTG 70 80 90 100 110 120 KKSQAQAQVH LQEVKGLGQS YIDKVQPEVE ELEPAPSGPG QQVADKHELG RLQYSLDYDF 130 140 150 160 170 180 QSGQLLVGIL QAMGLAALDL GGSSDPYVRV YLLPDKRRRY ETKVHRQTLN PHFGETFAFK 190 200 210 220 230 240 VPYVELGGRV LVMAVYDFDR FSRNDAIGEV RVPMSSVDLG RPVQAWRELQ AAPREEQEKL 250 260 270 280 290 300 GDICFSLRYV PTAGKLTVIV LEAKNLKKMD VGGLSDPYVK VHLLQGGKKV RKKKTTIKKN 310 320 330 340 350 360 TLNPYYNEAF SFEVPCDQVQ KVQVELTVLD YDKLGKNEAI GRVAVGAAAG GAGLRHWADM 370 380 LANPRRPIAQ WHSLRPPDRV RLLPAP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Human synaptotagmin V (SYT5): sequence, genomic structure, and chromosomal location." Craxton M.A., Olsen A., Goedert M. Genomics 42:165-169(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Brain.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[3]	"The DNA sequence and biology of human chromosome 19." Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M. Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT GLN-111. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X96783 Genomic DNA. Translation: CAA65579.1. AK125182 mRNA. Translation: BAG54160.1. AC010327 Genomic DNA. No translation available. BC046157 mRNA. Translation: AAH46157.1.
IPI	IPI00011727.
RefSeq	NP_003171.2. NM_003180.2.
UniGene	Hs.631566.
3D structure databases
ProteinModelPortal	O00445.
ModBase	Search...
Protein-protein interaction databases
IntAct	O00445. 1 interaction.
MINT	MINT-1388417.
STRING	9606.ENSP00000346265.
PTM databases
PhosphoSite	O00445.
Proteomic databases
PaxDb	O00445.
PRIDE	O00445.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000354308; ENSP00000346265; ENSG00000129990. ENST00000537500; ENSP00000442896; ENSG00000129990.
GeneID	6861.
KEGG	hsa:6861.
UCSC	uc002qjm.1. human.
Organism-specific databases
CTD	6861.
GeneCards	GC19M055684.
HGNC	HGNC:11513. SYT5.
HPA	HPA010688.
MIM	600782. gene.
neXtProt	NX_O00445.
PharmGKB	PA36294.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG292488.
HOGENOM	HOG000232127.
HOVERGEN	HBG005010.
InParanoid	O00445.
OMA	RSYIDKV.
OrthoDB	EOG4JHCFZ.
PhylomeDB	O00445.
Enzyme and pathway databases
Reactome	REACT_111217. Metabolism.
Gene expression databases
ArrayExpress	O00445.
Bgee	O00445.
CleanEx	HS_SYT5.
Genevestigator	O00445.
GermOnline	ENSG00000129990. Homo sapiens.
Family and domain databases
InterPro	IPR000008. C2_Ca-dep. IPR008973. C2_Ca/lipid-bd_dom_CaLB. IPR020477. C2_dom. IPR018029. C2_membr_targeting. IPR001565. Synaptotagmin. [Graphical view]
Pfam	PF00168. C2. 2 hits. [Graphical view]
PRINTS	PR00360. C2DOMAIN. PR00399. SYNAPTOTAGMN.
SMART	SM00239. C2. 2 hits. [Graphical view]
SUPFAM	SSF49562. C2_CaLB. 2 hits.
PROSITE	PS50004. C2. 2 hits. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	6861.
NextBio	26775.
SOURCE	Search...

Entry information

Entry name

SYT5_HUMAN

Accession

Primary (citable) accession number: O00445
Secondary accession number(s): B3KWJ8, Q86X72

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	July 11, 2003
Last modified:	May 1, 2013
This is version 118 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.