Reviewed, UniProtKB/Swiss-Prot O00444 (PLK4_HUMAN)

Names and origin

Protein names

Recommended name:
    Serine/threonine-protein kinase PLK4
    EC=2.7.11.21
Alternative name(s):
    Polo-like kinase 4
      Short name=PLK-4
    Serine/threonine-protein kinase Sak
    Serine/threonine-protein kinase 18

Gene names

Name:	PLK4
Synonyms:	SAK, STK18

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	970 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May play a role in cell proliferation.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Post-translational modification	Tyrosine-phosphorylated by TEC. Ref.2 Ref.4
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily. Contains 1 POLO box domain. Contains 1 protein kinase domain.

Ontologies

Keywords
Coding sequence diversity	Polymorphism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Traceable author statement. Source: ProtInc
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct protein serine/threonine kinase activity Traceable author statement. Source: ProtInc protein tyrosine kinase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
SFN	P31947	1	EBI-746202,EBI-476295

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 970

970

Serine/threonine-protein kinase PLK4

PRO_0000086567

Regions

Domain

12 – 265

254

Protein kinase

Domain

892 – 956

65

POLO box

Nucleotide binding

18 – 26

9

ATP By similarity

Sites

Active site

136

1

Proton acceptor By similarity

Binding site

41

1

ATP By similarity

Amino acid modifications

Modified residue

401

1

Phosphoserine Ref.4

Modified residue

421

1

Phosphoserine Ref.4

Modified residue

499

1

Phosphoserine Ref.4

Modified residue

592

1

Phosphoserine Ref.4

Modified residue

671

1

Phosphoserine Ref.4

Modified residue

817

1

Phosphoserine Ref.4

Natural variations

Natural variant

86

1

Y → C: dbSNP rs34156294. Ref.5

VAR_041027

Natural variant

146

1

R → H: dbSNP rs35232579. Ref.5

VAR_041028

Natural variant

226

1

A → T Ref.5

VAR_041029

Natural variant

232

1

S → T: dbSNP rs3811740. Ref.2 Ref.5 Ref.1

VAR_019632

Natural variant

317

1

P → L Ref.5

VAR_041030

Natural variant

449

1

N → D Ref.5

VAR_041031

Natural variant

519

1

W → S Ref.5

VAR_041032

Natural variant

830

1

E → D: dbSNP rs17012739. Ref.2 Ref.5 Ref.1

VAR_041033

Experimental info

Sequence conflict

34

1

T → S in BAB69958. Ref.2

Sequence conflict

58

1

Q → K in CAA73575. Ref.1

Sequence conflict

387

1

S → R in BAB69958. Ref.2

Sequence conflict

696

1

V → L in BAB69958. Ref.2

Sequence conflict

768

1

Y → F in CAA73575. Ref.1

Sequence conflict

842

1

A → D in BAB69958. Ref.2

Secondary structure

1

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970

Helix Strand Turn

Details...

Helix

2 – 5

4

Helix

9 – 11

3

Beta strand

12 – 20

9

Beta strand

22 – 31

10

Turn

32 – 34

3

Beta strand

37 – 44

8

Helix

45 – 50

6

Helix

54 – 64

11

Beta strand

75 – 80

6

Beta strand

82 – 90

9

Helix

97 – 102

6

Helix

110 – 129

20

Helix

139 – 141

3

Beta strand

142 – 144

3

Beta strand

150 – 152

3

Helix

193 – 206

14

Helix

236 – 245

10

Helix

250 – 252

3

Helix

256 – 259

4

Turn

263 – 265

3

Sequences

	Sequence		Length	Mass (Da)
	O00444-1 [UniParc]. Last modified November 13, 2007. Version 3. Checksum: 4D56F5FD983211A6	FASTA	970	108,972
	10 20 30 40 50 60 MATCIGEKIE DFKVGNLLGK GSFAGVYRAE SIHTGLEVAI KMIDKKAMYK AGMVQRVQNE 70 80 90 100 110 120 VKIHCQLKHP SILELYNYFE DSNYVYLVLE MCHNGEMNRY LKNRVKPFSE NEARHFMHQI 130 140 150 160 170 180 ITGMLYLHSH GILHRDLTLS NLLLTRNMNI KIADFGLATQ LKMPHEKHYT LCGTPNYISP 190 200 210 220 230 240 EIATRSAHGL ESDVWSLGCM FYTLLIGRPP FDTDTVKNTL NKVVLADYEM PSFLSIEAKD 250 260 270 280 290 300 LIHQLLRRNP ADRLSLSSVL DHPFMSRNSS TKSKDLGTVE DSIDSGHATI STAITASSST 310 320 330 340 350 360 SISGSLFDKR RLLIGQPLPN KMTVFPKNKS STDFSSSGDG NSFYTQWGNQ ETSNSGRGRV 370 380 390 400 410 420 IQDAEERPHS RYLRRAYSSD RSGTSNSQSQ AKTYTMERCH SAEMLSVSKR SGGGENEERY 430 440 450 460 470 480 SPTDNNANIF NFFKEKTSSS SGSFERPDNN QALSNHLCPG KTPFPFADPT PQTETVQQWF 490 500 510 520 530 540 GNLQINAHLR KTTEYDSISP NRDFQGHPDL QKDTSKNAWT DTKVKKNSDA SDNAHSVKQQ 550 560 570 580 590 600 NTMKYMTALH SKPEIIQQEC VFGSDPLSEQ SKTRGMEPPW GYQNRTLRSI TSPLVAHRLK 610 620 630 640 650 660 PIRQKTKKAV VSILDSEEVC VELVKEYASQ EYVKEVLQIS SDGNTITIYY PNGGRGFPLA 670 680 690 700 710 720 DRPPSPTDNI SRYSFDNLPE KYWRKYQYAS RFVQLVRSKS PKITYFTRYA KCILMENSPG 730 740 750 760 770 780 ADFEVWFYDG VKIHKTEDFI QVIEKTGKSY TLKSESEVNS LKEEIKMYMD HANEGHRICL 790 800 810 820 830 840 ALESIISEEE RKTRSAPFFP IIIGRKPGST SSPKALSPPP SVDSNYPTRE RASFNRMVMH 850 860 870 880 890 900 SAASPTQAPI LNPSMVTNEG LGLTTTASGT DISSNSLKDC LPKSAQLLKS VFVKNVGWAT 910 920 930 940 950 960 QLTSGAVWVQ FNDGSQLVVQ AGVSSISYTS PNGQTTRYGE NEKLPDYIKQ KLQCLSSILL 970 MFSNPTPNFH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Human SAK related to the PLK/polo family of cell cycle kinases shows high mRNA expression in testis." Karn T., Holtrich U., Wolf G., Hock B., Strebhardt K., Ruebsamen-Waigmann H. Oncol. Rep. 4:505-510(1997) Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS THR-232 AND ASP-830. Tissue: Lung.
[2]	"Sak serine-threonine kinase acts as an effector of Tec tyrosine kinase." Yamashita Y., Kajigaya S., Yoshida K., Ueno S., Ota J., Ohmine K., Ueda M., Miyazato A., Ohya K., Kitamura T., Ozawa K., Mano H. J. Biol. Chem. 276:39012-39020(2001) [PubMed: 11489907] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PHOSPHORYLATION BY TEC, VARIANTS THR-232 AND ASP-830. Tissue: Blood.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
[4]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed: 18691976] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-401; SER-421; SER-499; SER-592; SER-671 AND SER-817, MASS SPECTROMETRY.
[5]	"Patterns of somatic mutation in human cancer genomes." Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G. , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R. Nature 446:153-158(2007) [PubMed: 17344846] [Abstract] Cited for: VARIANTS [LARGE SCALE ANALYSIS] CYS-86; HIS-146; THR-226; THR-232; LEU-317; ASP-449; SER-519 AND ASP-830.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

Y13115 mRNA. Translation: CAA73575.1.
AB006972 mRNA. Translation: BAB69958.1.
BC036023 mRNA. Translation: AAH36023.1.

IPI

IPI00410344.

RefSeq

NP_055079.3.

UniGene

Hs.172052

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3COK	X-ray	2.25	A/B	2-275	[»]

SMR

O00444. Positions 890-964.

ModBase

Search...

Protein-protein interaction databases

IntAct

O00444. 9 interactions.

PTM databases

PhosphoSite

O00444.

Proteomic databases

PRIDE

O00444.

Genome annotation databases

Ensembl

ENSG00000142731. Homo sapiens. [Contig view]

GeneID

10733.

KEGG

hsa:10733.

UCSC

uc003ifo.1. human.

Organism-specific databases

GeneCards

GC04P129021.

HGNC

HGNC:11397. PLK4.

MIM

605031. gene.

PharmGKB

PA36205.

GenAtlas

Search...

Phylogenomic databases

HOGENOM

O00444.

HOVERGEN

O00444.

OMA

O00444. YTLKSES.

Enzyme and pathway databases

BRENDA

2.7.11.21. 247.

Reactome

REACT_152. Cell Cycle, Mitotic.

Gene expression databases

ArrayExpress

O00444.

Bgee

O00444.

CleanEx

HS_PLK4.

GermOnline

ENSG00000142731. Homo sapiens.

Family and domain databases

InterPro

IPR000959. POLO_box_duplicated.
IPR000719. Prot_kinase_core.
IPR017441. Protein_kinase_ATP_BS.
IPR015114. Sak_Polo.
IPR017442. Se/Thr_pkinase-rel.
IPR002290. Ser_thr_pkinase.
IPR008266. Tyr_pkinase_AS.
[Graphical view]

Pfam

PF00069. Pkinase. 1 hit.
PF09024. Sak_Polo. 1 hit.
[Graphical view]

ProDom

PD000001. Prot_kinase. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SMART

SM00220. S_TKc. 1 hit.
[Graphical view]

PROSITE

PS50078. POLO_BOX. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

BindingDB

O00444.

NextBio

40744.

SOURCE

Search...

Entry information

Entry name

PLK4_HUMAN

Accession

Primary (citable) accession number: O00444
Secondary accession number(s): Q8IYF0, Q96Q95

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2004
Last sequence update:	November 13, 2007
Last modified:	July 7, 2009
This is version 76 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)