Reviewed, UniProtKB/Swiss-Prot O00442 (RTC1_HUMAN)

Names and origin

Protein names

Recommended name:
    RNA 3'-terminal phosphate cyclase
      Short name=RNA-3'-phosphate cyclase
      Short name=RNA cyclase
    EC=6.5.1.4
Alternative name(s):
    RNA terminal phosphate cyclase domain-containing protein 1
      Short name=RTC domain-containing protein 1

Gene names

Name:	RTCD1
Synonyms:	RPC, RPC1, RTC1

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	366 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) the enzyme acts on RNA-N3'P to produce RNA-N3'PP5'A; (C) a non catalytic nucleophilic attack by the adjacent 2'hydroxyl on the phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing.
Catalytic activity	ATP + RNA 3'-terminal-phosphate = AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate.
Subunit structure	Monomer.
Subcellular location	Nucleus › nucleoplasm.
Tissue specificity	Ubiquitous.
Sequence similarities	Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily.

Ontologies

Keywords
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	RNA processing Inferred from electronic annotation. Source: InterPro
Cellular component	nucleoplasm Ref.1 Traceable author statement. Source: ProtInc
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Ref.1 Traceable author statement. Source: ProtInc RNA-3'-phosphate cyclase activity Ref.1 Traceable author statement. Source: ProtInc
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O00442-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O00442-2)

The sequence of this isoform differs from the canonical sequence as follows:
     48-48: L → LSSGGWKSKIKVLT

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 366	366	RNA 3'-terminal phosphate cyclase		PRO_0000156410
Sites
	Active site	320	1	By similarity
Natural variations
	Alternative sequence	48	1	L → LSSGGWKSKIKVLT in isoform 2.		VSP_005915
Experimental info
	Sequence conflict	34	1	L → S in AAH12604. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: D129E680FF08BAD1	FASTA	366	39,337
	10 20 30 40 50 60 MAGPRVEVDG SIMEGGGQIL RVSTALSCLL GLPLRVQKIR AGRSTPGLRP QHLSGLEMIR 70 80 90 100 110 120 DLCDGQLEGA EIGSTEITFT PEKIKGGIHT ADTKTAGSVC LLMQVSMPCV LFAASPSELH 130 140 150 160 170 180 LKGGTNAEMA PQIDYTVMVF KPIVEKFGFI FNCDIKTRGY YPKGGGEVIV RMSPVKQLNP 190 200 210 220 230 240 INLTERGCVT KIYGRAFVAG VLPFKVAKDM AAAAVRCIRK EIRDLYVNIQ PVQEPKDQAF 250 260 270 280 290 300 GNGNGIIIIA ETSTGCLFAG SSLGKRGVNA DKVGIEAAEM LLANLRHGGT VDEYLQDQLI 310 320 330 340 350 360 VFMALANGVS RIKTGPVTLH TQTAIHFAEQ IAKAKFIVKK SEDEEDAAKD TYIIECQGIG MTNPNL
	Isoform 2. Checksum: 1AD20D4CDDF4D0E6	FASTA	379	40,709
	10 20 30 40 50 60 MAGPRVEVDG SIMEGGGQIL RVSTALSCLL GLPLRVQKIR AGRSTPGLSS GGWKSKIKVL 70 80 90 100 110 120 TRPQHLSGLE MIRDLCDGQL EGAEIGSTEI TFTPEKIKGG IHTADTKTAG SVCLLMQVSM 130 140 150 160 170 180 PCVLFAASPS ELHLKGGTNA EMAPQIDYTV MVFKPIVEKF GFIFNCDIKT RGYYPKGGGE 190 200 210 220 230 240 VIVRMSPVKQ LNPINLTERG CVTKIYGRAF VAGVLPFKVA KDMAAAAVRC IRKEIRDLYV 250 260 270 280 290 300 NIQPVQEPKD QAFGNGNGII IIAETSTGCL FAGSSLGKRG VNADKVGIEA AEMLLANLRH 310 320 330 340 350 360 GGTVDEYLQD QLIVFMALAN GVSRIKTGPV TLHTQTAIHF AEQIAKAKFI VKKSEDEEDA 370 AKDTYIIECQ GIGMTNPNL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The human RNA 3'-terminal phosphate cyclase is a member of a new family of proteins conserved in Eucarya, Bacteria and Archaea." Genschik P., Billy E., Swianiewicz M., Filipowicz W. EMBO J. 16:2955-2967(1997) [PubMed: 9184239] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1), PARTIAL PROTEIN SEQUENCE. Tissue: Blood.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Brain.
[3]	"RNA 3'-terminal phosphate cyclase from HeLa cells." Filipowicz W., Vincente O. Methods Enzymol. 181:499-510(1990) [PubMed: 2199762] [Abstract] Cited for: CHARACTERIZATION.
[4]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Y11651 mRNA. Translation: CAA72364.1. Y11652 Genomic DNA. Translation: CAA72365.1. BC012604 mRNA. Translation: AAH12604.1.
IPI	IPI00011726. IPI00221327.
PIR	T48844.
RefSeq	NP_001124313.1. NP_003720.1.
UniGene	Hs.552835
3D structure databases
HSSP	HSSP built from PDB template 1QMH based on UniProtKB P46849.
ModBase	Search...
Protein-protein interaction databases
STRING	O00442.
Proteomic databases
PRIDE	O00442.
Genome annotation databases
Ensembl	ENST00000260563; ENSP00000260563; ENSG00000137996; Homo sapiens. [Genome view] ENST00000370126; ENSP00000359144; ENSG00000137996; Homo sapiens. [Genome view] ENST00000370128; ENSP00000359146; ENSG00000137996; Homo sapiens. [Genome view]
GeneID	8634.
KEGG	hsa:8634.
UCSC	uc001dtc.1. human.
Organism-specific databases
CTD	8634.
GeneCards	GC01P100504.
H-InvDB	HIX0000816.
HGNC	HGNC:17981. RTCD1.
MIM	611286. gene.
PharmGKB	PA34877.
GenAtlas	Search...
Phylogenomic databases
HOVERGEN	O00442.
OMA	EIDGSYG.
Enzyme and pathway databases
BRENDA	6.5.1.4. 247.
Gene expression databases
ArrayExpress	O00442.
Bgee	O00442.
CleanEx	HS_RTCD1.
Genevestigator	O00442.
Family and domain databases
InterPro	IPR000228. RNA3'_term_phos_cycl-like. IPR013796. RNA3'_term_phos_cycl_insert. IPR017770. RNA3'_term_phos_cycl_sub. [Graphical view]
Gene3D	G3DSA:3.65.10.20. RNA3'_term_phos_cycl. 1 hit.
PANTHER	PTHR11096. RNA3'_term_phos_cycl. 1 hit.
Pfam	PF01137. RTC. 1 hit. PF05189. RTC_insert. 1 hit. [Graphical view]
TIGRFAMs	TIGR03399. RNA_3prim_cycl. 1 hit.
PROSITE	PS01287. RTC. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	32367.
PMAP-CutDB	O00442.
SOURCE	Search...

Entry information

Entry name

RTC1_HUMAN

Accession

Primary (citable) accession number: O00442
Secondary accession number(s): Q96E99

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	July 1, 1997
Last modified:	November 3, 2009
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.