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Reviewed, UniProtKB/Swiss-Prot O00442 (RTC1_HUMAN)

Last modified November 3, 2009. Version 87. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    RNA 3'-terminal phosphate cyclase
      Short name=RNA-3'-phosphate cyclase
      Short name=RNA cyclase
    EC=6.5.1.4
Alternative name(s):
    RNA terminal phosphate cyclase domain-containing protein 1
      Short name=RTC domain-containing protein 1
Gene names
Name: RTCD1
Synonyms: RPC, RPC1, RTC1
OrganismHomo sapiens (Human) [Complete proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length366 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) the enzyme acts on RNA-N3'P to produce RNA-N3'PP5'A; (C) a non catalytic nucleophilic attack by the adjacent 2'hydroxyl on the phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing.

Catalytic activity

ATP + RNA 3'-terminal-phosphate = AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate.

Subunit structure

Monomer.

Subcellular location

Nucleusnucleoplasm.

Tissue specificity

Ubiquitous.

Sequence similarities

Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily.

Ontologies

Keywords
   Cellular componentNucleus
   Coding sequence diversityAlternative splicing
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Direct protein sequencing
Gene Ontology (GO)
   Biological processRNA processing

Inferred from electronic annotation. Source: InterPro

   Cellular componentnucleoplasm Ref.1

Traceable author statement. Source: ProtInc

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

RNA binding Ref.1

Traceable author statement. Source: ProtInc

RNA-3'-phosphate cyclase activity Ref.1

Traceable author statement. Source: ProtInc

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: O00442-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: O00442-2)

The sequence of this isoform differs from the canonical sequence as follows:
     48-48: L → LSSGGWKSKIKVLT
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 366366RNA 3'-terminal phosphate cyclase
PRO_0000156410

Sites

Active site3201 By similarity

Natural variations

Alternative sequence481L → LSSGGWKSKIKVLT in isoform 2.
VSP_005915

Experimental info

Sequence conflict341L → S in AAH12604. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified July 1, 1997. Version 1.
Checksum: D129E680FF08BAD1

FASTA36639,337
        10         20         30         40         50         60 
MAGPRVEVDG SIMEGGGQIL RVSTALSCLL GLPLRVQKIR AGRSTPGLRP QHLSGLEMIR 

        70         80         90        100        110        120 
DLCDGQLEGA EIGSTEITFT PEKIKGGIHT ADTKTAGSVC LLMQVSMPCV LFAASPSELH 

       130        140        150        160        170        180 
LKGGTNAEMA PQIDYTVMVF KPIVEKFGFI FNCDIKTRGY YPKGGGEVIV RMSPVKQLNP 

       190        200        210        220        230        240 
INLTERGCVT KIYGRAFVAG VLPFKVAKDM AAAAVRCIRK EIRDLYVNIQ PVQEPKDQAF 

       250        260        270        280        290        300 
GNGNGIIIIA ETSTGCLFAG SSLGKRGVNA DKVGIEAAEM LLANLRHGGT VDEYLQDQLI 

       310        320        330        340        350        360 
VFMALANGVS RIKTGPVTLH TQTAIHFAEQ IAKAKFIVKK SEDEEDAAKD TYIIECQGIG 


MTNPNL 

« Hide

Isoform 2.

Checksum: 1AD20D4CDDF4D0E6
Show »

FASTA37940,709

References

« Hide 'large scale' references
[1]"The human RNA 3'-terminal phosphate cyclase is a member of a new family of proteins conserved in Eucarya, Bacteria and Archaea."
Genschik P., Billy E., Swianiewicz M., Filipowicz W.
EMBO J. 16:2955-2967(1997) [PubMed: 9184239] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1), PARTIAL PROTEIN SEQUENCE.
Tissue: Blood.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Brain.
[3]"RNA 3'-terminal phosphate cyclase from HeLa cells."
Filipowicz W., Vincente O.
Methods Enzymol. 181:499-510(1990) [PubMed: 2199762] [Abstract]
Cited for: CHARACTERIZATION.
[4]Colinge J., Superti-Furga G., Bennett K.L.
Submitted (OCT-2008) to UniProtKB
Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.

Cross-references

Sequence databases

Y11651 mRNA. Translation: CAA72364.1.
Y11652 Genomic DNA. Translation: CAA72365.1.
BC012604 mRNA. Translation: AAH12604.1.
IPIIPI00011726.
IPI00221327.
PIRT48844.
RefSeqNP_001124313.1.
NP_003720.1.
UniGeneHs.552835

3D structure databases

HSSPHSSP built from PDB template 1QMH based on UniProtKB P46849.
ModBaseSearch...

Protein-protein interaction databases

STRINGO00442.

Proteomic databases

PRIDEO00442.

Genome annotation databases

EnsemblENST00000260563; ENSP00000260563; ENSG00000137996; Homo sapiens. [Genome view]
ENST00000370126; ENSP00000359144; ENSG00000137996; Homo sapiens. [Genome view]
ENST00000370128; ENSP00000359146; ENSG00000137996; Homo sapiens. [Genome view]
GeneID8634.
KEGGhsa:8634.
UCSCuc001dtc.1. human.

Organism-specific databases

CTD8634.
GeneCardsGC01P100504.
H-InvDBHIX0000816.
HGNCHGNC:17981. RTCD1.
MIM611286. gene.
PharmGKBPA34877.
GenAtlasSearch...

Phylogenomic databases

HOVERGENO00442.
OMAEIDGSYG.

Enzyme and pathway databases

BRENDA6.5.1.4. 247.

Gene expression databases

ArrayExpressO00442.
BgeeO00442.
CleanExHS_RTCD1.
GenevestigatorO00442.

Family and domain databases

InterProIPR000228. RNA3'_term_phos_cycl-like.
IPR013796. RNA3'_term_phos_cycl_insert.
IPR017770. RNA3'_term_phos_cycl_sub.
[Graphical view]
Gene3DG3DSA:3.65.10.20. RNA3'_term_phos_cycl. 1 hit.
PANTHERPTHR11096. RNA3'_term_phos_cycl. 1 hit.
PfamPF01137. RTC. 1 hit.
PF05189. RTC_insert. 1 hit.
[Graphical view]
TIGRFAMsTIGR03399. RNA_3prim_cycl. 1 hit.
PROSITEPS01287. RTC. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio32367.
PMAP-CutDBO00442.
SOURCESearch...

Entry information

Entry nameRTC1_HUMAN
AccessionPrimary (citable) accession number: O00442
Secondary accession number(s): Q96E99
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 1, 1997
Last modified: November 3, 2009
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

Human chromosome 1

Human chromosome 1: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents