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O00442

- RTCA_HUMAN

UniProt

O00442 - RTCA_HUMAN

Protein

RNA 3'-terminal phosphate cyclase

Gene

RTCA

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 134 (01 Oct 2014)
      Sequence version 1 (01 Jul 1997)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing.

    Catalytic activityi

    ATP + RNA 3'-terminal-phosphate = AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei104 – 1041ATPBy similarity
    Active sitei320 – 3201Tele-AMP-histidine intermediateBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi294 – 2985ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. poly(A) RNA binding Source: UniProtKB
    3. RNA-3'-phosphate cyclase activity Source: ProtInc
    4. RNA binding Source: ProtInc

    GO - Biological processi

    1. RNA processing Source: InterPro

    Keywords - Molecular functioni

    Ligase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    RNA 3'-terminal phosphate cyclase (EC:6.5.1.4)
    Short name:
    RNA cyclase
    Short name:
    RNA-3'-phosphate cyclase
    Alternative name(s):
    RNA terminal phosphate cyclase domain-containing protein 1
    Short name:
    RTC domain-containing protein 1
    Gene namesi
    Name:RTCA
    Synonyms:RPC, RPC1, RTC1, RTCD1
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 1

    Organism-specific databases

    HGNCiHGNC:17981. RTCA.

    Subcellular locationi

    GO - Cellular componenti

    1. nucleoplasm Source: ProtInc
    2. nucleus Source: HPA

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA34877.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 366366RNA 3'-terminal phosphate cyclasePRO_0000156410Add
    BLAST

    Proteomic databases

    MaxQBiO00442.
    PaxDbiO00442.
    PRIDEiO00442.

    PTM databases

    PhosphoSiteiO00442.

    Miscellaneous databases

    PMAP-CutDBO00442.

    Expressioni

    Tissue specificityi

    Ubiquitous.

    Gene expression databases

    ArrayExpressiO00442.
    BgeeiO00442.
    CleanExiHS_RTCD1.
    GenevestigatoriO00442.

    Organism-specific databases

    HPAiHPA027982.
    HPA028151.

    Interactioni

    Subunit structurei

    Monomer.

    Protein-protein interaction databases

    BioGridi114187. 11 interactions.
    IntActiO00442. 1 interaction.
    STRINGi9606.ENSP00000260563.

    Structurei

    3D structure databases

    ProteinModelPortaliO00442.
    SMRiO00442. Positions 6-336.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0430.
    HOGENOMiHOG000015264.
    HOVERGENiHBG017761.
    KOiK01974.
    OMAiAEMLLRN.
    OrthoDBiEOG780RMG.
    PhylomeDBiO00442.
    TreeFamiTF300831.

    Family and domain databases

    Gene3Di3.30.360.20. 1 hit.
    3.65.10.20. 2 hits.
    InterProiIPR013791. RNA3'-term_phos_cycl_insert.
    IPR023797. RNA3'_phos_cyclase_dom.
    IPR000228. RNA3'_term_phos_cyc.
    IPR017770. RNA3'_term_phos_cyc_type_1.
    IPR020719. RNA3'_term_phos_cycl-like_CS.
    IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b.
    [Graphical view]
    PANTHERiPTHR11096. PTHR11096. 1 hit.
    PfamiPF01137. RTC. 1 hit.
    PF05189. RTC_insert. 1 hit.
    [Graphical view]
    SUPFAMiSSF52913. SSF52913. 1 hit.
    SSF55205. SSF55205. 2 hits.
    TIGRFAMsiTIGR03399. RNA_3prim_cycl. 1 hit.
    PROSITEiPS01287. RTC. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: O00442-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAGPRVEVDG SIMEGGGQIL RVSTALSCLL GLPLRVQKIR AGRSTPGLRP    50
    QHLSGLEMIR DLCDGQLEGA EIGSTEITFT PEKIKGGIHT ADTKTAGSVC 100
    LLMQVSMPCV LFAASPSELH LKGGTNAEMA PQIDYTVMVF KPIVEKFGFI 150
    FNCDIKTRGY YPKGGGEVIV RMSPVKQLNP INLTERGCVT KIYGRAFVAG 200
    VLPFKVAKDM AAAAVRCIRK EIRDLYVNIQ PVQEPKDQAF GNGNGIIIIA 250
    ETSTGCLFAG SSLGKRGVNA DKVGIEAAEM LLANLRHGGT VDEYLQDQLI 300
    VFMALANGVS RIKTGPVTLH TQTAIHFAEQ IAKAKFIVKK SEDEEDAAKD 350
    TYIIECQGIG MTNPNL 366
    Length:366
    Mass (Da):39,337
    Last modified:July 1, 1997 - v1
    Checksum:iD129E680FF08BAD1
    GO
    Isoform 2 (identifier: O00442-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         48-48: L → LSSGGWKSKIKVLT

    Note: No experimental confirmation available.

    Show »
    Length:379
    Mass (Da):40,709
    Checksum:i1AD20D4CDDF4D0E6
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti34 – 341L → S in AAH12604. (PubMed:15489334)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei48 – 481L → LSSGGWKSKIKVLT in isoform 2. 1 PublicationVSP_005915

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y11651 mRNA. Translation: CAA72364.1.
    Y11652 Genomic DNA. Translation: CAA72365.1.
    AL445928, AL663111 Genomic DNA. Translation: CAH72259.1.
    AL445928, AL663111 Genomic DNA. Translation: CAH72260.1.
    AL663111, AL445928 Genomic DNA. Translation: CAH73852.1.
    AL663111, AL445928 Genomic DNA. Translation: CAH73853.1.
    CH471097 Genomic DNA. Translation: EAW72961.1.
    CH471097 Genomic DNA. Translation: EAW72962.1.
    BC012604 mRNA. Translation: AAH12604.1.
    CCDSiCCDS44178.1. [O00442-2]
    CCDS768.1. [O00442-1]
    PIRiT48844.
    RefSeqiNP_001124313.1. NM_001130841.1. [O00442-2]
    NP_003720.1. NM_003729.3. [O00442-1]
    UniGeneiHs.484222.

    Genome annotation databases

    EnsembliENST00000260563; ENSP00000260563; ENSG00000137996. [O00442-2]
    ENST00000370128; ENSP00000359146; ENSG00000137996. [O00442-1]
    GeneIDi8634.
    KEGGihsa:8634.
    UCSCiuc001dtc.3. human. [O00442-1]
    uc001dtd.3. human. [O00442-2]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y11651 mRNA. Translation: CAA72364.1 .
    Y11652 Genomic DNA. Translation: CAA72365.1 .
    AL445928 , AL663111 Genomic DNA. Translation: CAH72259.1 .
    AL445928 , AL663111 Genomic DNA. Translation: CAH72260.1 .
    AL663111 , AL445928 Genomic DNA. Translation: CAH73852.1 .
    AL663111 , AL445928 Genomic DNA. Translation: CAH73853.1 .
    CH471097 Genomic DNA. Translation: EAW72961.1 .
    CH471097 Genomic DNA. Translation: EAW72962.1 .
    BC012604 mRNA. Translation: AAH12604.1 .
    CCDSi CCDS44178.1. [O00442-2 ]
    CCDS768.1. [O00442-1 ]
    PIRi T48844.
    RefSeqi NP_001124313.1. NM_001130841.1. [O00442-2 ]
    NP_003720.1. NM_003729.3. [O00442-1 ]
    UniGenei Hs.484222.

    3D structure databases

    ProteinModelPortali O00442.
    SMRi O00442. Positions 6-336.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 114187. 11 interactions.
    IntActi O00442. 1 interaction.
    STRINGi 9606.ENSP00000260563.

    PTM databases

    PhosphoSitei O00442.

    Proteomic databases

    MaxQBi O00442.
    PaxDbi O00442.
    PRIDEi O00442.

    Protocols and materials databases

    DNASUi 8634.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000260563 ; ENSP00000260563 ; ENSG00000137996 . [O00442-2 ]
    ENST00000370128 ; ENSP00000359146 ; ENSG00000137996 . [O00442-1 ]
    GeneIDi 8634.
    KEGGi hsa:8634.
    UCSCi uc001dtc.3. human. [O00442-1 ]
    uc001dtd.3. human. [O00442-2 ]

    Organism-specific databases

    CTDi 8634.
    GeneCardsi GC01P100732.
    HGNCi HGNC:17981. RTCA.
    HPAi HPA027982.
    HPA028151.
    MIMi 611286. gene.
    neXtProti NX_O00442.
    PharmGKBi PA34877.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG0430.
    HOGENOMi HOG000015264.
    HOVERGENi HBG017761.
    KOi K01974.
    OMAi AEMLLRN.
    OrthoDBi EOG780RMG.
    PhylomeDBi O00442.
    TreeFami TF300831.

    Miscellaneous databases

    ChiTaRSi RTCA. human.
    GenomeRNAii 8634.
    NextBioi 32367.
    PMAP-CutDB O00442.
    PROi O00442.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O00442.
    Bgeei O00442.
    CleanExi HS_RTCD1.
    Genevestigatori O00442.

    Family and domain databases

    Gene3Di 3.30.360.20. 1 hit.
    3.65.10.20. 2 hits.
    InterProi IPR013791. RNA3'-term_phos_cycl_insert.
    IPR023797. RNA3'_phos_cyclase_dom.
    IPR000228. RNA3'_term_phos_cyc.
    IPR017770. RNA3'_term_phos_cyc_type_1.
    IPR020719. RNA3'_term_phos_cycl-like_CS.
    IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b.
    [Graphical view ]
    PANTHERi PTHR11096. PTHR11096. 1 hit.
    Pfami PF01137. RTC. 1 hit.
    PF05189. RTC_insert. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52913. SSF52913. 1 hit.
    SSF55205. SSF55205. 2 hits.
    TIGRFAMsi TIGR03399. RNA_3prim_cycl. 1 hit.
    PROSITEi PS01287. RTC. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The human RNA 3'-terminal phosphate cyclase is a member of a new family of proteins conserved in Eucarya, Bacteria and Archaea."
      Genschik P., Billy E., Swianiewicz M., Filipowicz W.
      EMBO J. 16:2955-2967(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1), PARTIAL PROTEIN SEQUENCE.
      Tissue: Blood.
    2. "The DNA sequence and biological annotation of human chromosome 1."
      Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.
      , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
      Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Brain.
    5. "RNA 3'-terminal phosphate cyclase from HeLa cells."
      Filipowicz W., Vincente O.
      Methods Enzymol. 181:499-510(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiRTCA_HUMAN
    AccessioniPrimary (citable) accession number: O00442
    Secondary accession number(s): Q5VVL5, Q5VVL6, Q96E99
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: July 1, 1997
    Last modified: October 1, 2014
    This is version 134 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 1
      Human chromosome 1: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3