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O00442

- RTCA_HUMAN

UniProt

O00442 - RTCA_HUMAN

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Protein
RNA 3'-terminal phosphate cyclase
Gene
RTCA, RPC, RPC1, RTC1, RTCD1
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing.

Catalytic activityi

ATP + RNA 3'-terminal-phosphate = AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei104 – 1041ATP By similarity
Active sitei320 – 3201Tele-AMP-histidine intermediate By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi294 – 2985ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. RNA binding Source: ProtInc
  3. RNA-3'-phosphate cyclase activity Source: ProtInc
  4. poly(A) RNA binding Source: UniProtKB
Complete GO annotation...

GO - Biological processi

  1. RNA processing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
RNA 3'-terminal phosphate cyclase (EC:6.5.1.4)
Short name:
RNA cyclase
Short name:
RNA-3'-phosphate cyclase
Alternative name(s):
RNA terminal phosphate cyclase domain-containing protein 1
Short name:
RTC domain-containing protein 1
Gene namesi
Name:RTCA
Synonyms:RPC, RPC1, RTC1, RTCD1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 1

Organism-specific databases

HGNCiHGNC:17981. RTCA.

Subcellular locationi

GO - Cellular componenti

  1. nucleoplasm Source: ProtInc
  2. nucleus Source: HPA
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA34877.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 366366RNA 3'-terminal phosphate cyclase
PRO_0000156410Add
BLAST

Proteomic databases

MaxQBiO00442.
PaxDbiO00442.
PRIDEiO00442.

PTM databases

PhosphoSiteiO00442.

Miscellaneous databases

PMAP-CutDBO00442.

Expressioni

Tissue specificityi

Ubiquitous.

Gene expression databases

ArrayExpressiO00442.
BgeeiO00442.
CleanExiHS_RTCD1.
GenevestigatoriO00442.

Organism-specific databases

HPAiHPA027982.
HPA028151.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

BioGridi114187. 11 interactions.
IntActiO00442. 1 interaction.
STRINGi9606.ENSP00000260563.

Structurei

3D structure databases

ProteinModelPortaliO00442.
SMRiO00442. Positions 6-336.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0430.
HOGENOMiHOG000015264.
HOVERGENiHBG017761.
KOiK01974.
OMAiAEMLLRN.
OrthoDBiEOG780RMG.
PhylomeDBiO00442.
TreeFamiTF300831.

Family and domain databases

Gene3Di3.30.360.20. 1 hit.
3.65.10.20. 2 hits.
InterProiIPR013791. RNA3'-term_phos_cycl_insert.
IPR023797. RNA3'_phos_cyclase_dom.
IPR000228. RNA3'_term_phos_cyc.
IPR017770. RNA3'_term_phos_cyc_type_1.
IPR020719. RNA3'_term_phos_cycl-like_CS.
IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b.
[Graphical view]
PANTHERiPTHR11096. PTHR11096. 1 hit.
PfamiPF01137. RTC. 1 hit.
PF05189. RTC_insert. 1 hit.
[Graphical view]
SUPFAMiSSF52913. SSF52913. 1 hit.
SSF55205. SSF55205. 2 hits.
TIGRFAMsiTIGR03399. RNA_3prim_cycl. 1 hit.
PROSITEiPS01287. RTC. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: O00442-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MAGPRVEVDG SIMEGGGQIL RVSTALSCLL GLPLRVQKIR AGRSTPGLRP    50
QHLSGLEMIR DLCDGQLEGA EIGSTEITFT PEKIKGGIHT ADTKTAGSVC 100
LLMQVSMPCV LFAASPSELH LKGGTNAEMA PQIDYTVMVF KPIVEKFGFI 150
FNCDIKTRGY YPKGGGEVIV RMSPVKQLNP INLTERGCVT KIYGRAFVAG 200
VLPFKVAKDM AAAAVRCIRK EIRDLYVNIQ PVQEPKDQAF GNGNGIIIIA 250
ETSTGCLFAG SSLGKRGVNA DKVGIEAAEM LLANLRHGGT VDEYLQDQLI 300
VFMALANGVS RIKTGPVTLH TQTAIHFAEQ IAKAKFIVKK SEDEEDAAKD 350
TYIIECQGIG MTNPNL 366
Length:366
Mass (Da):39,337
Last modified:July 1, 1997 - v1
Checksum:iD129E680FF08BAD1
GO
Isoform 2 (identifier: O00442-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     48-48: L → LSSGGWKSKIKVLT

Note: No experimental confirmation available.

Show »
Length:379
Mass (Da):40,709
Checksum:i1AD20D4CDDF4D0E6
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei48 – 481L → LSSGGWKSKIKVLT in isoform 2.
VSP_005915

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti34 – 341L → S in AAH12604. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y11651 mRNA. Translation: CAA72364.1.
Y11652 Genomic DNA. Translation: CAA72365.1.
AL445928, AL663111 Genomic DNA. Translation: CAH72259.1.
AL445928, AL663111 Genomic DNA. Translation: CAH72260.1.
AL663111, AL445928 Genomic DNA. Translation: CAH73852.1.
AL663111, AL445928 Genomic DNA. Translation: CAH73853.1.
CH471097 Genomic DNA. Translation: EAW72961.1.
CH471097 Genomic DNA. Translation: EAW72962.1.
BC012604 mRNA. Translation: AAH12604.1.
CCDSiCCDS44178.1. [O00442-2]
CCDS768.1. [O00442-1]
PIRiT48844.
RefSeqiNP_001124313.1. NM_001130841.1. [O00442-2]
NP_003720.1. NM_003729.3. [O00442-1]
UniGeneiHs.484222.

Genome annotation databases

EnsembliENST00000260563; ENSP00000260563; ENSG00000137996. [O00442-2]
ENST00000370128; ENSP00000359146; ENSG00000137996. [O00442-1]
GeneIDi8634.
KEGGihsa:8634.
UCSCiuc001dtc.3. human. [O00442-1]
uc001dtd.3. human. [O00442-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y11651 mRNA. Translation: CAA72364.1 .
Y11652 Genomic DNA. Translation: CAA72365.1 .
AL445928 , AL663111 Genomic DNA. Translation: CAH72259.1 .
AL445928 , AL663111 Genomic DNA. Translation: CAH72260.1 .
AL663111 , AL445928 Genomic DNA. Translation: CAH73852.1 .
AL663111 , AL445928 Genomic DNA. Translation: CAH73853.1 .
CH471097 Genomic DNA. Translation: EAW72961.1 .
CH471097 Genomic DNA. Translation: EAW72962.1 .
BC012604 mRNA. Translation: AAH12604.1 .
CCDSi CCDS44178.1. [O00442-2 ]
CCDS768.1. [O00442-1 ]
PIRi T48844.
RefSeqi NP_001124313.1. NM_001130841.1. [O00442-2 ]
NP_003720.1. NM_003729.3. [O00442-1 ]
UniGenei Hs.484222.

3D structure databases

ProteinModelPortali O00442.
SMRi O00442. Positions 6-336.
ModBasei Search...

Protein-protein interaction databases

BioGridi 114187. 11 interactions.
IntActi O00442. 1 interaction.
STRINGi 9606.ENSP00000260563.

PTM databases

PhosphoSitei O00442.

Proteomic databases

MaxQBi O00442.
PaxDbi O00442.
PRIDEi O00442.

Protocols and materials databases

DNASUi 8634.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000260563 ; ENSP00000260563 ; ENSG00000137996 . [O00442-2 ]
ENST00000370128 ; ENSP00000359146 ; ENSG00000137996 . [O00442-1 ]
GeneIDi 8634.
KEGGi hsa:8634.
UCSCi uc001dtc.3. human. [O00442-1 ]
uc001dtd.3. human. [O00442-2 ]

Organism-specific databases

CTDi 8634.
GeneCardsi GC01P100732.
HGNCi HGNC:17981. RTCA.
HPAi HPA027982.
HPA028151.
MIMi 611286. gene.
neXtProti NX_O00442.
PharmGKBi PA34877.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG0430.
HOGENOMi HOG000015264.
HOVERGENi HBG017761.
KOi K01974.
OMAi AEMLLRN.
OrthoDBi EOG780RMG.
PhylomeDBi O00442.
TreeFami TF300831.

Miscellaneous databases

ChiTaRSi RTCA. human.
GenomeRNAii 8634.
NextBioi 32367.
PMAP-CutDB O00442.
PROi O00442.
SOURCEi Search...

Gene expression databases

ArrayExpressi O00442.
Bgeei O00442.
CleanExi HS_RTCD1.
Genevestigatori O00442.

Family and domain databases

Gene3Di 3.30.360.20. 1 hit.
3.65.10.20. 2 hits.
InterProi IPR013791. RNA3'-term_phos_cycl_insert.
IPR023797. RNA3'_phos_cyclase_dom.
IPR000228. RNA3'_term_phos_cyc.
IPR017770. RNA3'_term_phos_cyc_type_1.
IPR020719. RNA3'_term_phos_cycl-like_CS.
IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b.
[Graphical view ]
PANTHERi PTHR11096. PTHR11096. 1 hit.
Pfami PF01137. RTC. 1 hit.
PF05189. RTC_insert. 1 hit.
[Graphical view ]
SUPFAMi SSF52913. SSF52913. 1 hit.
SSF55205. SSF55205. 2 hits.
TIGRFAMsi TIGR03399. RNA_3prim_cycl. 1 hit.
PROSITEi PS01287. RTC. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The human RNA 3'-terminal phosphate cyclase is a member of a new family of proteins conserved in Eucarya, Bacteria and Archaea."
    Genschik P., Billy E., Swianiewicz M., Filipowicz W.
    EMBO J. 16:2955-2967(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1), PARTIAL PROTEIN SEQUENCE.
    Tissue: Blood.
  2. "The DNA sequence and biological annotation of human chromosome 1."
    Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.
    , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
    Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Brain.
  5. "RNA 3'-terminal phosphate cyclase from HeLa cells."
    Filipowicz W., Vincente O.
    Methods Enzymol. 181:499-510(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiRTCA_HUMAN
AccessioniPrimary (citable) accession number: O00442
Secondary accession number(s): Q5VVL5, Q5VVL6, Q96E99
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 1, 1997
Last modified: July 9, 2014
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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