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Protein

Histone deacetylase complex subunit SAP18

Gene

SAP18

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the SIN3-repressing complex. Enhances the ability of SIN3-HDAC1-mediated transcriptional repression. When tethered to the promoter, it can direct the formation of a repressive complex to core histone proteins. Auxiliary component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Component of the ASAP and PSAP complexes which bind RNA in a sequence-independent manner and are proposed to be recruited to the EJC prior to or during the splicing process and to regulate specific excision of introns in specific transcription subsets. The ASAP complex can inhibit mRNA processing during in vitro splicing reactions. The ASAP complex promotes apoptosis and is disassembled after induction of apoptosis. Involved in the splicing modulation of BCL2L1/Bcl-X (and probably other apoptotic genes); specifically inhibits the formation of proapoptotic isoforms such as Bcl-X(S); the activity is different from the established EJC assembly and function.4 Publications

GO - Molecular functioni

  • histone deacetylase activity Source: Reactome
  • RNA binding Source: UniProtKB
  • transcription corepressor activity Source: ProtInc

GO - Biological processi

  • mRNA processing Source: UniProtKB-KW
  • negative regulation of mRNA splicing, via spliceosome Source: UniProtKB
  • positive regulation of apoptotic process Source: UniProtKB
  • regulation of alternative mRNA splicing, via spliceosome Source: UniProtKB
  • regulation of transcription by RNA polymerase II Source: ProtInc
  • RNA splicing Source: UniProtKB-KW
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionRepressor
Biological processmRNA processing, mRNA splicing, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-HSA-3214815 HDACs deacetylate histones
R-HSA-427413 NoRC negatively regulates rRNA expression
SignaLinkiO00422
SIGNORiO00422

Protein family/group databases

TCDBi3.A.18.1.1 the nuclear mrna exporter (mrna-e) family

Names & Taxonomyi

Protein namesi
Recommended name:
Histone deacetylase complex subunit SAP18
Alternative name(s):
18 kDa Sin3-associated polypeptide
2HOR0202
Cell growth-inhibiting gene 38 protein
Sin3-associated polypeptide p18
Gene namesi
Name:SAP18
ORF Names:GIG38
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 13

Organism-specific databases

EuPathDBiHostDB:ENSG00000150459.12
HGNCiHGNC:10530 SAP18
MIMi602949 gene
neXtProtiNX_O00422

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi118D → A: Abolishes splicing regulation activity and interaction with RNPS1 and ACIN1; when associated with A-121. 1 Publication1
Mutagenesisi121T → A: Abolishes splicing regulation activity and interaction with RNPS1 and ACIN1; when associated with A-118. 1 Publication1
Mutagenesisi126K → A: No effect on splicing regulation activity. 1 Publication1

Organism-specific databases

OpenTargetsiENSG00000150459
PharmGKBiPA34940

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00002209752 – 153Histone deacetylase complex subunit SAP18Add BLAST152

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Cross-linki13Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources

Keywords - PTMi

Acetylation, Isopeptide bond, Ubl conjugation

Proteomic databases

EPDiO00422
MaxQBiO00422
PaxDbiO00422
PeptideAtlasiO00422
PRIDEiO00422
ProteomicsDBi47879
TopDownProteomicsiO00422

PTM databases

iPTMnetiO00422
PhosphoSitePlusiO00422

Expressioni

Tissue specificityi

Ubiquitous.

Gene expression databases

BgeeiENSG00000150459
CleanExiHS_SAP18
ExpressionAtlasiO00422 baseline and differential
GenevisibleiO00422 HS

Organism-specific databases

HPAiCAB012273
HPA011352

Interactioni

Subunit structurei

Found in a mRNA splicing-dependent exon junction complex (EJC). Component of the heterotrimeric ASAP (apoptosis- and splicing-associated protein) and PSAP complexes consisting of RNPS1, SAP18 and either ACIN1 or PNN, respectively; the ASAP and PSAP complexes probably are formed mutually exclusive. For the ASAP complex, the association of SAP18 seems to require a preformed RNPS1:ACIN1 complex. Forms a complex with SIN3A and HDAC1. Interacts with SUFU.5 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi115573, 150 interactors
CORUMiO00422
DIPiDIP-33590N
IntActiO00422, 26 interactors
MINTiO00422
STRINGi9606.ENSP00000371973

Structurei

Secondary structure

1153
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni22 – 24Combined sources3
Beta strandi28 – 37Combined sources10
Helixi43 – 45Combined sources3
Turni52 – 54Combined sources3
Beta strandi55 – 60Combined sources6
Helixi66 – 76Combined sources11
Helixi78 – 81Combined sources4
Beta strandi86 – 91Combined sources6
Beta strandi95 – 98Combined sources4
Beta strandi104 – 112Combined sources9
Helixi122 – 125Combined sources4
Beta strandi132 – 138Combined sources7
Beta strandi144 – 147Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2HDENMR-A6-149[»]
ProteinModelPortaliO00422
SMRiO00422
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO00422

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni93 – 153Involved in splicing regulation activityAdd BLAST61

Sequence similaritiesi

Belongs to the SAP18 family.Curated

Phylogenomic databases

eggNOGiKOG3391 Eukaryota
ENOG4111FBB LUCA
GeneTreeiENSGT00390000003152
HOGENOMiHOG000238745
HOVERGENiHBG003465
InParanoidiO00422
KOiK14324
PhylomeDBiO00422
TreeFamiTF313032

Family and domain databases

InterProiView protein in InterPro
IPR017250 Hist_deAcase_cplx_SAP18
IPR010516 SAP18
PANTHERiPTHR13082 PTHR13082, 1 hit
PfamiView protein in Pfam
PF06487 SAP18, 1 hit
PIRSFiPIRSF037637 HDAC_SAP18, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O00422-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVESRVTQE EIKKEPEKPI DREKTCPLLL RVFTTNNGRH HRMDEFSRGN
60 70 80 90 100
VPSSELQIYT WMDATLKELT SLVKEVYPEA RKKGTHFNFA IVFTDVKRPG
110 120 130 140 150
YRVKEIGSTM SGRKGTDDSM TLQSQKFQIG DYLDIAITPP NRAPPPSGRM

RPY
Length:153
Mass (Da):17,561
Last modified:July 1, 1997 - v1
Checksum:iC7E479FFE9CA5774
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti114K → E in AAF21220 (Ref. 2) Curated1
Sequence conflicti146P → T in AAH30836 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U96915 mRNA Translation: AAC51322.1
U78303 mRNA Translation: AAF21220.1
AF153608 mRNA Translation: AAD41090.1
AY550970 mRNA Translation: AAT52216.1
CR457035 mRNA Translation: CAG33316.1
AK311748 mRNA Translation: BAG34691.1
AL158032 Genomic DNA No translation available.
CH471075 Genomic DNA Translation: EAX08291.1
BC030836 mRNA Translation: AAH30836.1
RefSeqiNP_005861.2, NM_005870.4
UniGeneiHs.524899

Genome annotation databases

EnsembliENST00000607003; ENSP00000475925; ENSG00000150459
GeneIDi10284
KEGGihsa:10284
UCSCiuc058vux.1 human

Similar proteinsi

Entry informationi

Entry nameiSAP18_HUMAN
AccessioniPrimary (citable) accession number: O00422
Secondary accession number(s): B2R494
, Q2TTR4, Q6IAW9, Q8N606, Q9UF14
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: July 1, 1997
Last modified: June 20, 2018
This is version 166 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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