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Protein

Histone deacetylase complex subunit SAP18

Gene

SAP18

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the SIN3-repressing complex. Enhances the ability of SIN3-HDAC1-mediated transcriptional repression. When tethered to the promoter, it can direct the formation of a repressive complex to core histone proteins. Auxiliary component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Component of the ASAP and PSAP complexes which bind RNA in a sequence-independent manner and are proposed to be recruited to the EJC prior to or during the splicing process and to regulate specific excision of introns in specific transcription subsets. The ASAP complex can inhibit mRNA processing during in vitro splicing reactions. The ASAP complex promotes apoptosis and is disassembled after induction of apoptosis. Involved in the splicing modulation of BCL2L1/Bcl-X (and probably other apoptotic genes); specifically inhibits the formation of proapoptotic isoforms such as Bcl-X(S); the activity is different from the established EJC assembly and function.4 Publications

GO - Molecular functioni

  • histone deacetylase activity Source: Reactome
  • poly(A) RNA binding Source: UniProtKB
  • transcription corepressor activity Source: ProtInc

GO - Biological processi

  • mRNA processing Source: UniProtKB-KW
  • negative regulation of mRNA splicing, via spliceosome Source: UniProtKB
  • positive regulation of apoptotic process Source: UniProtKB
  • regulation of alternative mRNA splicing, via spliceosome Source: UniProtKB
  • regulation of transcription from RNA polymerase II promoter Source: ProtInc
  • RNA splicing Source: UniProtKB-KW
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

mRNA processing, mRNA splicing, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-HSA-3214815. HDACs deacetylate histones.
R-HSA-427413. NoRC negatively regulates rRNA expression.
SignaLinkiO00422.
SIGNORiO00422.

Protein family/group databases

TCDBi3.A.18.1.1. the nuclear mrna exporter (mrna-e) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Histone deacetylase complex subunit SAP18
Alternative name(s):
18 kDa Sin3-associated polypeptide
2HOR0202
Cell growth-inhibiting gene 38 protein
Sin3-associated polypeptide p18
Gene namesi
Name:SAP18
ORF Names:GIG38
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 13

Organism-specific databases

HGNCiHGNC:10530. SAP18.

Subcellular locationi

GO - Cellular componenti

  • ASAP complex Source: UniProtKB
  • cytoplasm Source: HPA
  • histone deacetylase complex Source: ProtInc
  • nuclear speck Source: UniProtKB
  • nucleoplasm Source: HPA
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi118 – 1181D → A: Abolishes splicing regulation activity and interaction with RNPS1 and ACIN1; when associated with A-121. 1 Publication
Mutagenesisi121 – 1211T → A: Abolishes splicing regulation activity and interaction with RNPS1 and ACIN1; when associated with A-118. 1 Publication
Mutagenesisi126 – 1261K → A: No effect on splicing regulation activity. 1 Publication

Organism-specific databases

PharmGKBiPA34940.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedCombined sources
Chaini2 – 153152Histone deacetylase complex subunit SAP18PRO_0000220975Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineCombined sources
Cross-linki13 – 13Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources

Keywords - PTMi

Acetylation, Isopeptide bond, Ubl conjugation

Proteomic databases

EPDiO00422.
MaxQBiO00422.
PaxDbiO00422.
PeptideAtlasiO00422.
PRIDEiO00422.
TopDownProteomicsiO00422.

PTM databases

iPTMnetiO00422.
PhosphoSiteiO00422.

Expressioni

Tissue specificityi

Ubiquitous.

Gene expression databases

BgeeiENSG00000150459.
CleanExiHS_SAP18.
ExpressionAtlasiO00422. baseline and differential.
GenevisibleiO00422. HS.

Organism-specific databases

HPAiCAB012273.
HPA011352.

Interactioni

Subunit structurei

Found in a mRNA splicing-dependent exon junction complex (EJC). Component of the heterotrimeric ASAP (apoptosis- and splicing-associated protein) and PSAP complexes consisting of RNPS1, SAP18 and either ACIN1 or PNN, respectively; the ASAP and PSAP complexes probably are formed mutually exclusive. For the ASAP complex, the association of SAP18 seems to require a preformed RNPS1:ACIN1 complex. Forms a complex with SIN3A and HDAC1. Interacts with SUFU.5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
HDAC1Q135472EBI-1044156,EBI-301834
INCA1Q0VD863EBI-1044156,EBI-6509505
RBM39Q144984EBI-1044156,EBI-395290
RBM39Q14498-33EBI-1044156,EBI-6654703

Protein-protein interaction databases

BioGridi115573. 101 interactions.
DIPiDIP-33590N.
IntActiO00422. 20 interactions.
MINTiMINT-5002140.
STRINGi9606.ENSP00000371973.

Structurei

Secondary structure

1
153
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni22 – 243Combined sources
Beta strandi28 – 3710Combined sources
Helixi43 – 453Combined sources
Turni52 – 543Combined sources
Beta strandi55 – 606Combined sources
Helixi66 – 7611Combined sources
Helixi78 – 814Combined sources
Beta strandi86 – 916Combined sources
Beta strandi95 – 984Combined sources
Beta strandi104 – 1129Combined sources
Helixi122 – 1254Combined sources
Beta strandi132 – 1387Combined sources
Beta strandi144 – 1474Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2HDENMR-A6-149[»]
ProteinModelPortaliO00422.
SMRiO00422. Positions 6-149.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO00422.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni93 – 15361Involved in splicing regulation activityAdd
BLAST

Sequence similaritiesi

Belongs to the SAP18 family.Curated

Phylogenomic databases

eggNOGiKOG3391. Eukaryota.
ENOG4111FBB. LUCA.
GeneTreeiENSGT00390000003152.
HOGENOMiHOG000238745.
HOVERGENiHBG003465.
InParanoidiO00422.
KOiK14324.
PhylomeDBiO00422.
TreeFamiTF313032.

Family and domain databases

InterProiIPR017250. Hist_deAcase_cplx_SAP18.
IPR010516. SAP18.
[Graphical view]
PANTHERiPTHR13082. PTHR13082. 1 hit.
PfamiPF06487. SAP18. 1 hit.
[Graphical view]
PIRSFiPIRSF037637. HDAC_SAP18. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O00422-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVESRVTQE EIKKEPEKPI DREKTCPLLL RVFTTNNGRH HRMDEFSRGN
60 70 80 90 100
VPSSELQIYT WMDATLKELT SLVKEVYPEA RKKGTHFNFA IVFTDVKRPG
110 120 130 140 150
YRVKEIGSTM SGRKGTDDSM TLQSQKFQIG DYLDIAITPP NRAPPPSGRM

RPY
Length:153
Mass (Da):17,561
Last modified:July 1, 1997 - v1
Checksum:iC7E479FFE9CA5774
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti114 – 1141K → E in AAF21220 (Ref. 2) Curated
Sequence conflicti146 – 1461P → T in AAH30836 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U96915 mRNA. Translation: AAC51322.1.
U78303 mRNA. Translation: AAF21220.1.
AF153608 mRNA. Translation: AAD41090.1.
AY550970 mRNA. Translation: AAT52216.1.
CR457035 mRNA. Translation: CAG33316.1.
AK311748 mRNA. Translation: BAG34691.1.
AL158032 Genomic DNA. Translation: CAH69949.1.
CH471075 Genomic DNA. Translation: EAX08291.1.
BC030836 mRNA. Translation: AAH30836.1.
RefSeqiNP_005861.2. NM_005870.4.
UniGeneiHs.524899.

Genome annotation databases

EnsembliENST00000607003; ENSP00000475925; ENSG00000150459.
GeneIDi10284.
KEGGihsa:10284.
UCSCiuc058vux.1. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U96915 mRNA. Translation: AAC51322.1.
U78303 mRNA. Translation: AAF21220.1.
AF153608 mRNA. Translation: AAD41090.1.
AY550970 mRNA. Translation: AAT52216.1.
CR457035 mRNA. Translation: CAG33316.1.
AK311748 mRNA. Translation: BAG34691.1.
AL158032 Genomic DNA. Translation: CAH69949.1.
CH471075 Genomic DNA. Translation: EAX08291.1.
BC030836 mRNA. Translation: AAH30836.1.
RefSeqiNP_005861.2. NM_005870.4.
UniGeneiHs.524899.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2HDENMR-A6-149[»]
ProteinModelPortaliO00422.
SMRiO00422. Positions 6-149.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115573. 101 interactions.
DIPiDIP-33590N.
IntActiO00422. 20 interactions.
MINTiMINT-5002140.
STRINGi9606.ENSP00000371973.

Protein family/group databases

TCDBi3.A.18.1.1. the nuclear mrna exporter (mrna-e) family.

PTM databases

iPTMnetiO00422.
PhosphoSiteiO00422.

Proteomic databases

EPDiO00422.
MaxQBiO00422.
PaxDbiO00422.
PeptideAtlasiO00422.
PRIDEiO00422.
TopDownProteomicsiO00422.

Protocols and materials databases

DNASUi10284.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000607003; ENSP00000475925; ENSG00000150459.
GeneIDi10284.
KEGGihsa:10284.
UCSCiuc058vux.1. human.

Organism-specific databases

CTDi10284.
GeneCardsiSAP18.
H-InvDBHIX0011163.
HGNCiHGNC:10530. SAP18.
HPAiCAB012273.
HPA011352.
MIMi602949. gene.
neXtProtiNX_O00422.
PharmGKBiPA34940.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3391. Eukaryota.
ENOG4111FBB. LUCA.
GeneTreeiENSGT00390000003152.
HOGENOMiHOG000238745.
HOVERGENiHBG003465.
InParanoidiO00422.
KOiK14324.
PhylomeDBiO00422.
TreeFamiTF313032.

Enzyme and pathway databases

ReactomeiR-HSA-3214815. HDACs deacetylate histones.
R-HSA-427413. NoRC negatively regulates rRNA expression.
SignaLinkiO00422.
SIGNORiO00422.

Miscellaneous databases

ChiTaRSiSAP18. human.
EvolutionaryTraceiO00422.
GeneWikiiSAP18.
GenomeRNAii10284.
PROiO00422.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000150459.
CleanExiHS_SAP18.
ExpressionAtlasiO00422. baseline and differential.
GenevisibleiO00422. HS.

Family and domain databases

InterProiIPR017250. Hist_deAcase_cplx_SAP18.
IPR010516. SAP18.
[Graphical view]
PANTHERiPTHR13082. PTHR13082. 1 hit.
PfamiPF06487. SAP18. 1 hit.
[Graphical view]
PIRSFiPIRSF037637. HDAC_SAP18. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSAP18_HUMAN
AccessioniPrimary (citable) accession number: O00422
Secondary accession number(s): B2R494
, Q2TTR4, Q6IAW9, Q8N606, Q9UF14
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: July 1, 1997
Last modified: September 7, 2016
This is version 153 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 13
    Human chromosome 13: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.