Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

O00411

- RPOM_HUMAN

UniProt

O00411 - RPOM_HUMAN

Protein

DNA-directed RNA polymerase, mitochondrial

Gene

POLRMT

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 132 (01 Oct 2014)
      Sequence version 2 (19 Jul 2004)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    DNA-dependent RNA polymerase catalyzes the transcription of mitochondrial DNA into RNA using the four ribonucleoside triphosphates as substrates.1 Publication

    Catalytic activityi

    Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei922 – 9221By similarity
    Active sitei991 – 9911By similarity
    Active sitei1151 – 11511By similarity

    GO - Molecular functioni

    1. DNA binding Source: InterPro
    2. DNA-directed RNA polymerase activity Source: UniProtKB
    3. poly(A) RNA binding Source: UniProtKB
    4. protein binding Source: UniProtKB

    GO - Biological processi

    1. gene expression Source: Reactome
    2. transcription from mitochondrial promoter Source: UniProtKB
    3. transcription initiation from mitochondrial promoter Source: Reactome

    Keywords - Molecular functioni

    Nucleotidyltransferase, Transferase

    Keywords - Biological processi

    Transcription

    Enzyme and pathway databases

    ReactomeiREACT_200608. Transcriptional activation of mitochondrial biogenesis.
    REACT_367. Mitochondrial transcription initiation.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA-directed RNA polymerase, mitochondrial (EC:2.7.7.6)
    Short name:
    MtRPOL
    Gene namesi
    Name:POLRMT
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 19

    Organism-specific databases

    HGNCiHGNC:9200. POLRMT.

    Subcellular locationi

    GO - Cellular componenti

    1. mitochondrial matrix Source: Reactome
    2. mitochondrial nucleoid Source: BHF-UCL
    3. mitochondrion Source: HGNC

    Keywords - Cellular componenti

    DNA-directed RNA polymerase, Mitochondrion

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA33522.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 4141MitochondrionSequence AnalysisAdd
    BLAST
    Chaini42 – 12301189DNA-directed RNA polymerase, mitochondrialPRO_0000031068Add
    BLAST

    Proteomic databases

    MaxQBiO00411.
    PaxDbiO00411.
    PRIDEiO00411.

    PTM databases

    PhosphoSiteiO00411.

    Expressioni

    Gene expression databases

    ArrayExpressiO00411.
    BgeeiO00411.
    CleanExiHS_POLRMT.
    GenevestigatoriO00411.

    Organism-specific databases

    HPAiHPA006366.

    Interactioni

    Subunit structurei

    Interacts with TFB1M and TFB2M, leading to the stimulation of transcription. Interacts with TEFM.2 Publications

    Protein-protein interaction databases

    BioGridi111438. 10 interactions.
    DIPiDIP-56412N.
    IntActiO00411. 4 interactions.
    MINTiMINT-1142772.
    STRINGi9606.ENSP00000215591.

    Structurei

    Secondary structure

    1
    1230
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi221 – 23616
    Helixi239 – 25113
    Helixi253 – 2564
    Helixi261 – 27414
    Helixi277 – 28913
    Helixi296 – 30914
    Helixi313 – 32513
    Helixi331 – 3366
    Helixi341 – 35111
    Helixi352 – 3543
    Turni372 – 3743
    Helixi375 – 3784
    Beta strandi380 – 3823
    Helixi393 – 40917
    Beta strandi411 – 4155
    Helixi427 – 46135
    Helixi469 – 4724
    Helixi476 – 48914
    Helixi497 – 51923
    Helixi522 – 53312
    Helixi534 – 5363
    Helixi549 – 5579
    Helixi562 – 5643
    Helixi569 – 58618
    Beta strandi588 – 5903
    Beta strandi604 – 6107
    Beta strandi613 – 6164
    Beta strandi619 – 6235
    Helixi625 – 63410
    Beta strandi637 – 6426
    Helixi643 – 6453
    Beta strandi648 – 6503
    Beta strandi653 – 6575
    Beta strandi660 – 6667
    Helixi677 – 6859
    Helixi689 – 6924
    Helixi693 – 70311
    Beta strandi706 – 7094
    Helixi711 – 72111
    Turni722 – 7243
    Helixi727 – 7293
    Helixi735 – 7373
    Beta strandi748 – 7503
    Helixi772 – 78918
    Beta strandi798 – 8003
    Beta strandi806 – 8094
    Beta strandi811 – 8133
    Helixi819 – 8235
    Beta strandi825 – 8295
    Turni834 – 8363
    Helixi837 – 84913
    Helixi857 – 87620
    Turni878 – 8803
    Helixi884 – 8874
    Beta strandi888 – 8903
    Helixi891 – 90515
    Beta strandi907 – 9093
    Helixi910 – 9123
    Beta strandi918 – 9225
    Helixi926 – 9349
    Helixi938 – 9436
    Helixi955 – 97218
    Helixi976 – 9805
    Turni981 – 9833
    Helixi987 – 99913
    Beta strandi1002 – 10043
    Helixi1005 – 101511
    Beta strandi1018 – 10203
    Turni1022 – 10243
    Helixi1025 – 104319
    Helixi1045 – 106319
    Beta strandi1069 – 10713
    Beta strandi1077 – 10793
    Helixi1112 – 114130
    Beta strandi1147 – 11493
    Beta strandi1152 – 11565
    Helixi1157 – 11593
    Helixi1160 – 117617
    Helixi1179 – 119113
    Helixi1198 – 120912
    Helixi1219 – 12246
    Beta strandi1228 – 12303

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3SPAX-ray2.50A105-1230[»]
    4BOCX-ray2.65A151-1230[»]
    ProteinModelPortaliO00411.
    SMRiO00411. Positions 218-1230.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni802 – 1230429Mediates interaction with TEFMAdd
    BLAST

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG5108.
    HOGENOMiHOG000044061.
    HOVERGENiHBG078730.
    InParanoidiO00411.
    KOiK10908.
    OMAiREMFTST.
    PhylomeDBiO00411.
    TreeFamiTF105700.

    Family and domain databases

    Gene3Di1.10.1320.10. 1 hit.
    1.10.287.260. 1 hit.
    InterProiIPR024075. DNA-dir_RNA_pol_helix_hairpin.
    IPR002092. DNA-dir_Rpol_phage-type.
    IPR029262. RPOL_N.
    [Graphical view]
    PANTHERiPTHR10102. PTHR10102. 1 hit.
    PfamiPF00940. RNA_pol. 1 hit.
    PF14700. RPOL_N. 1 hit.
    [Graphical view]
    PROSITEiPS00900. RNA_POL_PHAGE_1. 1 hit.
    PS00489. RNA_POL_PHAGE_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O00411-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSALCWGRGA AGLKRALRPC GRPGLPGKEG TAGGVCGPRR SSSASPQEQD     50
    QDRRKDWGHV ELLEVLQARV RQLQAESVSE VVVNRVDVAR LPECGSGDGS 100
    LQPPRKVQMG AKDATPVPCG RWAKILEKDK RTQQMRMQRL KAKLQMPFQS 150
    GEFKALTRRL QVEPRLLSKQ MAGCLEDCTR QAPESPWEEQ LARLLQEAPG 200
    KLSLDVEQAP SGQHSQAQLS GQQQRLLAFF KCCLLTDQLP LAHHLLVVHH 250
    GQRQKRKLLT LDMYNAVMLG WARQGAFKEL VYVLFMVKDA GLTPDLLSYA 300
    AALQCMGRQD QDAGTIERCL EQMSQEGLKL QALFTAVLLS EEDRATVLKA 350
    VHKVKPTFSL PPQLPPPVNT SKLLRDVYAK DGRVSYPKLH LPLKTLQCLF 400
    EKQLHMELAS RVCVVSVEKP TLPSKEVKHA RKTLKTLRDQ WEKALCRALR 450
    ETKNRLEREV YEGRFSLYPF LCLLDEREVV RMLLQVLQAL PAQGESFTTL 500
    ARELSARTFS RHVVQRQRVS GQVQALQNHY RKYLCLLASD AEVPEPCLPR 550
    QYWEELGAPE ALREQPWPLP VQMELGKLLA EMLVQATQMP CSLDKPHRSS 600
    RLVPVLYHVY SFRNVQQIGI LKPHPAYVQL LEKAAEPTLT FEAVDVPMLC 650
    PPLPWTSPHS GAFLLSPTKL MRTVEGATQH QELLETCPPT ALHGALDALT 700
    QLGNCAWRVN GRVLDLVLQL FQAKGCPQLG VPAPPSEAPQ PPEAHLPHSA 750
    APARKAELRR ELAHCQKVAR EMHSLRAEAL YRLSLAQHLR DRVFWLPHNM 800
    DFRGRTYPCP PHFNHLGSDV ARALLEFAQG RPLGPHGLDW LKIHLVNLTG 850
    LKKREPLRKR LAFAEEVMDD ILDSADQPLT GRKWWMGAEE PWQTLACCME 900
    VANAVRASDP AAYVSHLPVH QDGSCNGLQH YAALGRDSVG AASVNLEPSD 950
    VPQDVYSGVA AQVEVFRRQD AQRGMRVAQV LEGFITRKVV KQTVMTVVYG 1000
    VTRYGGRLQI EKRLRELSDF PQEFVWEASH YLVRQVFKSL QEMFSGTRAI 1050
    QHWLTESARL ISHMGSVVEW VTPLGVPVIQ PYRLDSKVKQ IGGGIQSITY 1100
    THNGDISRKP NTRKQKNGFP PNFIHSLDSS HMMLTALHCY RKGLTFVSVH 1150
    DCYWTHAADV SVMNQVCREQ FVRLHSEPIL QDLSRFLVKR FCSEPQKILE 1200
    ASQLKETLQA VPKPGAFDLE QVKRSTYFFS 1230
    Length:1,230
    Mass (Da):138,620
    Last modified:July 19, 2004 - v2
    Checksum:i47AFBF2E0884AEFA
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti399 – 3991L → F in AAB58255. (PubMed:9097968)Curated
    Sequence conflicti983 – 9831G → S in AAB58255. (PubMed:9097968)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti555 – 5551E → A.
    Corresponds to variant rs2238549 [ dbSNP | Ensembl ].
    VAR_019427

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U75370 mRNA. Translation: AAB58255.1.
    AC004449 Genomic DNA. Translation: AAC06147.1.
    CCDSiCCDS12036.1.
    RefSeqiNP_005026.3. NM_005035.3.
    UniGeneiHs.254113.

    Genome annotation databases

    EnsembliENST00000588649; ENSP00000465759; ENSG00000099821.
    GeneIDi5442.
    KEGGihsa:5442.
    UCSCiuc002lpf.1. human.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U75370 mRNA. Translation: AAB58255.1 .
    AC004449 Genomic DNA. Translation: AAC06147.1 .
    CCDSi CCDS12036.1.
    RefSeqi NP_005026.3. NM_005035.3.
    UniGenei Hs.254113.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3SPA X-ray 2.50 A 105-1230 [» ]
    4BOC X-ray 2.65 A 151-1230 [» ]
    ProteinModelPortali O00411.
    SMRi O00411. Positions 218-1230.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 111438. 10 interactions.
    DIPi DIP-56412N.
    IntActi O00411. 4 interactions.
    MINTi MINT-1142772.
    STRINGi 9606.ENSP00000215591.

    PTM databases

    PhosphoSitei O00411.

    Proteomic databases

    MaxQBi O00411.
    PaxDbi O00411.
    PRIDEi O00411.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000588649 ; ENSP00000465759 ; ENSG00000099821 .
    GeneIDi 5442.
    KEGGi hsa:5442.
    UCSCi uc002lpf.1. human.

    Organism-specific databases

    CTDi 5442.
    GeneCardsi GC19M000617.
    H-InvDB HIX0027511.
    HIX0059707.
    HGNCi HGNC:9200. POLRMT.
    HPAi HPA006366.
    MIMi 601778. gene.
    neXtProti NX_O00411.
    PharmGKBi PA33522.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG5108.
    HOGENOMi HOG000044061.
    HOVERGENi HBG078730.
    InParanoidi O00411.
    KOi K10908.
    OMAi REMFTST.
    PhylomeDBi O00411.
    TreeFami TF105700.

    Enzyme and pathway databases

    Reactomei REACT_200608. Transcriptional activation of mitochondrial biogenesis.
    REACT_367. Mitochondrial transcription initiation.

    Miscellaneous databases

    GeneWikii POLRMT.
    GenomeRNAii 5442.
    NextBioi 21059.
    PROi O00411.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O00411.
    Bgeei O00411.
    CleanExi HS_POLRMT.
    Genevestigatori O00411.

    Family and domain databases

    Gene3Di 1.10.1320.10. 1 hit.
    1.10.287.260. 1 hit.
    InterProi IPR024075. DNA-dir_RNA_pol_helix_hairpin.
    IPR002092. DNA-dir_Rpol_phage-type.
    IPR029262. RPOL_N.
    [Graphical view ]
    PANTHERi PTHR10102. PTHR10102. 1 hit.
    Pfami PF00940. RNA_pol. 1 hit.
    PF14700. RPOL_N. 1 hit.
    [Graphical view ]
    PROSITEi PS00900. RNA_POL_PHAGE_1. 1 hit.
    PS00489. RNA_POL_PHAGE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification of the gene encoding the human mitochondrial RNA polymerase (h-mtRPOL) by cyberscreening of the Expressed Sequence Tags database."
      Tiranti V., Savoia A., Forti F., D'Apolito M.F., Centra M., Rocchi M., Zeviani M.
      Hum. Mol. Genet. 6:615-625(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Liver.
    2. "The DNA sequence and biology of human chromosome 19."
      Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V.
      , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
      Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "Mitochondrial transcription factors B1 and B2 activate transcription of human mtDNA."
      Falkenberg M., Gaspari M., Rantanen A., Trifunovic A., Larsson N.-G., Gustafsson C.M.
      Nat. Genet. 31:289-294(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH TFB1M AND TFB2M.
    4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    5. Cited for: FUNCTION, INTERACTION WITH TEFM.

    Entry informationi

    Entry nameiRPOM_HUMAN
    AccessioniPrimary (citable) accession number: O00411
    Secondary accession number(s): O60370
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: July 19, 2004
    Last modified: October 1, 2014
    This is version 132 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 19
      Human chromosome 19: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3