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Protein

DNA-directed RNA polymerase, mitochondrial

Gene

POLRMT

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of mitochondrial DNA into RNA using the four ribonucleoside triphosphates as substrates.1 Publication

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei922 – 9221By similarity
Active sitei991 – 9911By similarity
Active sitei1151 – 11511By similarity

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. DNA-directed RNA polymerase activity Source: UniProtKB
  3. poly(A) RNA binding Source: UniProtKB

GO - Biological processi

  1. gene expression Source: Reactome
  2. mitochondrion organization Source: Reactome
  3. organelle organization Source: Reactome
  4. transcription from mitochondrial promoter Source: UniProtKB
  5. transcription initiation from mitochondrial promoter Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

Transcription

Enzyme and pathway databases

ReactomeiREACT_264212. Transcriptional activation of mitochondrial biogenesis.
REACT_367. Mitochondrial transcription initiation.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase, mitochondrial (EC:2.7.7.6)
Short name:
MtRPOL
Gene namesi
Name:POLRMT
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 19

Organism-specific databases

HGNCiHGNC:9200. POLRMT.

Subcellular locationi

GO - Cellular componenti

  1. mitochondrial matrix Source: Reactome
  2. mitochondrial nucleoid Source: BHF-UCL
  3. mitochondrion Source: HGNC
Complete GO annotation...

Keywords - Cellular componenti

DNA-directed RNA polymerase, Mitochondrion

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA33522.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 4141MitochondrionSequence AnalysisAdd
BLAST
Chaini42 – 12301189DNA-directed RNA polymerase, mitochondrialPRO_0000031068Add
BLAST

Proteomic databases

MaxQBiO00411.
PaxDbiO00411.
PRIDEiO00411.

PTM databases

PhosphoSiteiO00411.

Expressioni

Gene expression databases

BgeeiO00411.
CleanExiHS_POLRMT.
ExpressionAtlasiO00411. baseline and differential.
GenevestigatoriO00411.

Organism-specific databases

HPAiHPA006366.

Interactioni

Subunit structurei

Interacts with TFB1M and TFB2M, leading to the stimulation of transcription. Interacts with TEFM.2 Publications

Protein-protein interaction databases

BioGridi111438. 16 interactions.
DIPiDIP-56412N.
IntActiO00411. 4 interactions.
MINTiMINT-1142772.
STRINGi9606.ENSP00000215591.

Structurei

Secondary structure

1
1230
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi221 – 23616Combined sources
Helixi239 – 25113Combined sources
Helixi253 – 2564Combined sources
Helixi261 – 27414Combined sources
Helixi277 – 28913Combined sources
Helixi296 – 30914Combined sources
Helixi313 – 32513Combined sources
Helixi331 – 3366Combined sources
Helixi341 – 35111Combined sources
Helixi352 – 3543Combined sources
Turni372 – 3743Combined sources
Helixi375 – 3784Combined sources
Beta strandi380 – 3823Combined sources
Helixi393 – 40917Combined sources
Beta strandi411 – 4155Combined sources
Helixi427 – 46135Combined sources
Helixi469 – 4724Combined sources
Helixi476 – 48914Combined sources
Helixi497 – 51923Combined sources
Helixi522 – 53312Combined sources
Helixi534 – 5363Combined sources
Helixi549 – 5579Combined sources
Helixi562 – 5643Combined sources
Helixi569 – 58618Combined sources
Beta strandi588 – 5903Combined sources
Beta strandi604 – 6107Combined sources
Beta strandi613 – 6164Combined sources
Beta strandi619 – 6235Combined sources
Helixi625 – 63410Combined sources
Beta strandi637 – 6426Combined sources
Helixi643 – 6453Combined sources
Beta strandi648 – 6503Combined sources
Beta strandi653 – 6575Combined sources
Beta strandi660 – 6667Combined sources
Helixi677 – 6859Combined sources
Helixi689 – 6924Combined sources
Helixi693 – 70311Combined sources
Beta strandi706 – 7094Combined sources
Helixi711 – 72111Combined sources
Turni722 – 7243Combined sources
Helixi727 – 7293Combined sources
Helixi735 – 7373Combined sources
Beta strandi748 – 7503Combined sources
Helixi772 – 78918Combined sources
Beta strandi798 – 8003Combined sources
Beta strandi806 – 8094Combined sources
Beta strandi811 – 8133Combined sources
Helixi819 – 8235Combined sources
Beta strandi825 – 8295Combined sources
Turni834 – 8363Combined sources
Helixi837 – 84913Combined sources
Helixi857 – 87620Combined sources
Turni878 – 8803Combined sources
Helixi884 – 8874Combined sources
Beta strandi888 – 8903Combined sources
Helixi891 – 90515Combined sources
Beta strandi907 – 9093Combined sources
Helixi910 – 9123Combined sources
Beta strandi918 – 9225Combined sources
Helixi926 – 9349Combined sources
Helixi938 – 9436Combined sources
Helixi955 – 97218Combined sources
Helixi976 – 9805Combined sources
Turni981 – 9833Combined sources
Helixi987 – 99913Combined sources
Beta strandi1002 – 10043Combined sources
Helixi1005 – 101511Combined sources
Beta strandi1018 – 10203Combined sources
Turni1022 – 10243Combined sources
Helixi1025 – 104319Combined sources
Helixi1045 – 106319Combined sources
Beta strandi1069 – 10713Combined sources
Beta strandi1077 – 10793Combined sources
Helixi1112 – 114130Combined sources
Beta strandi1147 – 11493Combined sources
Beta strandi1152 – 11565Combined sources
Helixi1157 – 11593Combined sources
Helixi1160 – 117617Combined sources
Helixi1179 – 119113Combined sources
Helixi1198 – 120912Combined sources
Helixi1219 – 12246Combined sources
Beta strandi1228 – 12303Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3SPAX-ray2.50A105-1230[»]
4BOCX-ray2.65A151-1230[»]
ProteinModelPortaliO00411.
SMRiO00411. Positions 218-1230.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni802 – 1230429Mediates interaction with TEFMAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG5108.
GeneTreeiENSGT00390000008060.
HOGENOMiHOG000044061.
HOVERGENiHBG078730.
InParanoidiO00411.
KOiK10908.
OMAiTVDTMNK.
PhylomeDBiO00411.
TreeFamiTF105700.

Family and domain databases

Gene3Di1.10.1320.10. 1 hit.
1.10.287.260. 1 hit.
InterProiIPR024075. DNA-dir_RNA_pol_helix_hairpin.
IPR002092. DNA-dir_Rpol_phage-type.
IPR029262. RPOL_N.
[Graphical view]
PANTHERiPTHR10102. PTHR10102. 1 hit.
PfamiPF00940. RNA_pol. 1 hit.
PF14700. RPOL_N. 1 hit.
[Graphical view]
PROSITEiPS00900. RNA_POL_PHAGE_1. 1 hit.
PS00489. RNA_POL_PHAGE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O00411-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSALCWGRGA AGLKRALRPC GRPGLPGKEG TAGGVCGPRR SSSASPQEQD
60 70 80 90 100
QDRRKDWGHV ELLEVLQARV RQLQAESVSE VVVNRVDVAR LPECGSGDGS
110 120 130 140 150
LQPPRKVQMG AKDATPVPCG RWAKILEKDK RTQQMRMQRL KAKLQMPFQS
160 170 180 190 200
GEFKALTRRL QVEPRLLSKQ MAGCLEDCTR QAPESPWEEQ LARLLQEAPG
210 220 230 240 250
KLSLDVEQAP SGQHSQAQLS GQQQRLLAFF KCCLLTDQLP LAHHLLVVHH
260 270 280 290 300
GQRQKRKLLT LDMYNAVMLG WARQGAFKEL VYVLFMVKDA GLTPDLLSYA
310 320 330 340 350
AALQCMGRQD QDAGTIERCL EQMSQEGLKL QALFTAVLLS EEDRATVLKA
360 370 380 390 400
VHKVKPTFSL PPQLPPPVNT SKLLRDVYAK DGRVSYPKLH LPLKTLQCLF
410 420 430 440 450
EKQLHMELAS RVCVVSVEKP TLPSKEVKHA RKTLKTLRDQ WEKALCRALR
460 470 480 490 500
ETKNRLEREV YEGRFSLYPF LCLLDEREVV RMLLQVLQAL PAQGESFTTL
510 520 530 540 550
ARELSARTFS RHVVQRQRVS GQVQALQNHY RKYLCLLASD AEVPEPCLPR
560 570 580 590 600
QYWEELGAPE ALREQPWPLP VQMELGKLLA EMLVQATQMP CSLDKPHRSS
610 620 630 640 650
RLVPVLYHVY SFRNVQQIGI LKPHPAYVQL LEKAAEPTLT FEAVDVPMLC
660 670 680 690 700
PPLPWTSPHS GAFLLSPTKL MRTVEGATQH QELLETCPPT ALHGALDALT
710 720 730 740 750
QLGNCAWRVN GRVLDLVLQL FQAKGCPQLG VPAPPSEAPQ PPEAHLPHSA
760 770 780 790 800
APARKAELRR ELAHCQKVAR EMHSLRAEAL YRLSLAQHLR DRVFWLPHNM
810 820 830 840 850
DFRGRTYPCP PHFNHLGSDV ARALLEFAQG RPLGPHGLDW LKIHLVNLTG
860 870 880 890 900
LKKREPLRKR LAFAEEVMDD ILDSADQPLT GRKWWMGAEE PWQTLACCME
910 920 930 940 950
VANAVRASDP AAYVSHLPVH QDGSCNGLQH YAALGRDSVG AASVNLEPSD
960 970 980 990 1000
VPQDVYSGVA AQVEVFRRQD AQRGMRVAQV LEGFITRKVV KQTVMTVVYG
1010 1020 1030 1040 1050
VTRYGGRLQI EKRLRELSDF PQEFVWEASH YLVRQVFKSL QEMFSGTRAI
1060 1070 1080 1090 1100
QHWLTESARL ISHMGSVVEW VTPLGVPVIQ PYRLDSKVKQ IGGGIQSITY
1110 1120 1130 1140 1150
THNGDISRKP NTRKQKNGFP PNFIHSLDSS HMMLTALHCY RKGLTFVSVH
1160 1170 1180 1190 1200
DCYWTHAADV SVMNQVCREQ FVRLHSEPIL QDLSRFLVKR FCSEPQKILE
1210 1220 1230
ASQLKETLQA VPKPGAFDLE QVKRSTYFFS
Length:1,230
Mass (Da):138,620
Last modified:July 19, 2004 - v2
Checksum:i47AFBF2E0884AEFA
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti399 – 3991L → F in AAB58255 (PubMed:9097968).Curated
Sequence conflicti983 – 9831G → S in AAB58255 (PubMed:9097968).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti555 – 5551E → A.
Corresponds to variant rs2238549 [ dbSNP | Ensembl ].
VAR_019427

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U75370 mRNA. Translation: AAB58255.1.
AC004449 Genomic DNA. Translation: AAC06147.1.
CCDSiCCDS12036.1.
RefSeqiNP_005026.3. NM_005035.3.
UniGeneiHs.254113.

Genome annotation databases

EnsembliENST00000588649; ENSP00000465759; ENSG00000099821.
GeneIDi5442.
KEGGihsa:5442.
UCSCiuc002lpf.1. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U75370 mRNA. Translation: AAB58255.1.
AC004449 Genomic DNA. Translation: AAC06147.1.
CCDSiCCDS12036.1.
RefSeqiNP_005026.3. NM_005035.3.
UniGeneiHs.254113.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3SPAX-ray2.50A105-1230[»]
4BOCX-ray2.65A151-1230[»]
ProteinModelPortaliO00411.
SMRiO00411. Positions 218-1230.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111438. 16 interactions.
DIPiDIP-56412N.
IntActiO00411. 4 interactions.
MINTiMINT-1142772.
STRINGi9606.ENSP00000215591.

Chemistry

ChEMBLiCHEMBL3120041.

PTM databases

PhosphoSiteiO00411.

Proteomic databases

MaxQBiO00411.
PaxDbiO00411.
PRIDEiO00411.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000588649; ENSP00000465759; ENSG00000099821.
GeneIDi5442.
KEGGihsa:5442.
UCSCiuc002lpf.1. human.

Organism-specific databases

CTDi5442.
GeneCardsiGC19M000617.
H-InvDBHIX0027511.
HIX0059707.
HGNCiHGNC:9200. POLRMT.
HPAiHPA006366.
MIMi601778. gene.
neXtProtiNX_O00411.
PharmGKBiPA33522.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG5108.
GeneTreeiENSGT00390000008060.
HOGENOMiHOG000044061.
HOVERGENiHBG078730.
InParanoidiO00411.
KOiK10908.
OMAiTVDTMNK.
PhylomeDBiO00411.
TreeFamiTF105700.

Enzyme and pathway databases

ReactomeiREACT_264212. Transcriptional activation of mitochondrial biogenesis.
REACT_367. Mitochondrial transcription initiation.

Miscellaneous databases

GeneWikiiPOLRMT.
GenomeRNAii5442.
NextBioi21059.
PROiO00411.
SOURCEiSearch...

Gene expression databases

BgeeiO00411.
CleanExiHS_POLRMT.
ExpressionAtlasiO00411. baseline and differential.
GenevestigatoriO00411.

Family and domain databases

Gene3Di1.10.1320.10. 1 hit.
1.10.287.260. 1 hit.
InterProiIPR024075. DNA-dir_RNA_pol_helix_hairpin.
IPR002092. DNA-dir_Rpol_phage-type.
IPR029262. RPOL_N.
[Graphical view]
PANTHERiPTHR10102. PTHR10102. 1 hit.
PfamiPF00940. RNA_pol. 1 hit.
PF14700. RPOL_N. 1 hit.
[Graphical view]
PROSITEiPS00900. RNA_POL_PHAGE_1. 1 hit.
PS00489. RNA_POL_PHAGE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of the gene encoding the human mitochondrial RNA polymerase (h-mtRPOL) by cyberscreening of the Expressed Sequence Tags database."
    Tiranti V., Savoia A., Forti F., D'Apolito M.F., Centra M., Rocchi M., Zeviani M.
    Hum. Mol. Genet. 6:615-625(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Liver.
  2. "The DNA sequence and biology of human chromosome 19."
    Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V.
    , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
    Nature 428:529-535(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "Mitochondrial transcription factors B1 and B2 activate transcription of human mtDNA."
    Falkenberg M., Gaspari M., Rantanen A., Trifunovic A., Larsson N.-G., Gustafsson C.M.
    Nat. Genet. 31:289-294(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH TFB1M AND TFB2M.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  5. Cited for: FUNCTION, INTERACTION WITH TEFM.

Entry informationi

Entry nameiRPOM_HUMAN
AccessioniPrimary (citable) accession number: O00411
Secondary accession number(s): O60370
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: July 19, 2004
Last modified: April 1, 2015
This is version 136 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.