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Protein

DNA-directed RNA polymerase, mitochondrial

Gene

POLRMT

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of mitochondrial DNA into RNA using the four ribonucleoside triphosphates as substrates.1 Publication

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei922By similarity1
Active sitei991By similarity1
Active sitei1151By similarity1

GO - Molecular functioni

  • DNA binding Source: InterPro
  • DNA-directed RNA polymerase activity Source: UniProtKB
  • poly(A) RNA binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

Transcription

Enzyme and pathway databases

BioCyciZFISH:HS01915-MONOMER.
ReactomeiR-HSA-163282. Mitochondrial transcription initiation.
R-HSA-2151201. Transcriptional activation of mitochondrial biogenesis.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase, mitochondrial (EC:2.7.7.6)
Short name:
MtRPOL
Gene namesi
Name:POLRMT
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

HGNCiHGNC:9200. POLRMT.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial matrix Source: Reactome
  • mitochondrial nucleoid Source: BHF-UCL
  • mitochondrion Source: HGNC
Complete GO annotation...

Keywords - Cellular componenti

DNA-directed RNA polymerase, Mitochondrion

Pathology & Biotechi

Organism-specific databases

DisGeNETi5442.
OpenTargetsiENSG00000099821.
PharmGKBiPA33522.

Chemistry databases

ChEMBLiCHEMBL3120041.

Polymorphism and mutation databases

BioMutaiPOLRMT.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 41MitochondrionSequence analysisAdd BLAST41
ChainiPRO_000003106842 – 1230DNA-directed RNA polymerase, mitochondrialAdd BLAST1189

Proteomic databases

EPDiO00411.
MaxQBiO00411.
PaxDbiO00411.
PeptideAtlasiO00411.
PRIDEiO00411.

PTM databases

iPTMnetiO00411.
PhosphoSitePlusiO00411.

Expressioni

Gene expression databases

BgeeiENSG00000099821.
CleanExiHS_POLRMT.
ExpressionAtlasiO00411. baseline and differential.
GenevisibleiO00411. HS.

Organism-specific databases

HPAiHPA006366.

Interactioni

Subunit structurei

Interacts with TFB1M and TFB2M, leading to the stimulation of transcription. Interacts with TEFM.2 Publications

Protein-protein interaction databases

BioGridi111438. 34 interactors.
DIPiDIP-56412N.
IntActiO00411. 14 interactors.
MINTiMINT-1142772.
STRINGi9606.ENSP00000215591.

Structurei

Secondary structure

11230
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi221 – 236Combined sources16
Helixi239 – 251Combined sources13
Helixi253 – 256Combined sources4
Helixi261 – 274Combined sources14
Helixi277 – 289Combined sources13
Helixi296 – 309Combined sources14
Helixi313 – 325Combined sources13
Helixi331 – 336Combined sources6
Helixi341 – 351Combined sources11
Helixi352 – 354Combined sources3
Turni372 – 374Combined sources3
Helixi375 – 378Combined sources4
Beta strandi380 – 382Combined sources3
Helixi393 – 409Combined sources17
Beta strandi411 – 415Combined sources5
Helixi427 – 461Combined sources35
Helixi469 – 472Combined sources4
Helixi476 – 489Combined sources14
Helixi497 – 519Combined sources23
Helixi522 – 533Combined sources12
Helixi534 – 536Combined sources3
Helixi549 – 557Combined sources9
Helixi562 – 564Combined sources3
Helixi569 – 586Combined sources18
Beta strandi588 – 590Combined sources3
Beta strandi604 – 610Combined sources7
Beta strandi613 – 616Combined sources4
Beta strandi619 – 623Combined sources5
Helixi625 – 634Combined sources10
Beta strandi637 – 642Combined sources6
Helixi643 – 645Combined sources3
Beta strandi648 – 650Combined sources3
Beta strandi653 – 657Combined sources5
Beta strandi660 – 666Combined sources7
Helixi677 – 685Combined sources9
Helixi689 – 692Combined sources4
Helixi693 – 703Combined sources11
Beta strandi706 – 709Combined sources4
Helixi711 – 721Combined sources11
Turni722 – 724Combined sources3
Helixi727 – 729Combined sources3
Helixi735 – 737Combined sources3
Beta strandi748 – 750Combined sources3
Helixi772 – 789Combined sources18
Beta strandi798 – 800Combined sources3
Beta strandi806 – 809Combined sources4
Beta strandi811 – 813Combined sources3
Helixi819 – 823Combined sources5
Beta strandi825 – 829Combined sources5
Turni834 – 836Combined sources3
Helixi837 – 849Combined sources13
Helixi857 – 876Combined sources20
Turni878 – 880Combined sources3
Helixi884 – 887Combined sources4
Beta strandi888 – 890Combined sources3
Helixi891 – 905Combined sources15
Beta strandi907 – 909Combined sources3
Helixi910 – 912Combined sources3
Beta strandi918 – 922Combined sources5
Helixi926 – 934Combined sources9
Helixi938 – 943Combined sources6
Helixi955 – 972Combined sources18
Helixi976 – 980Combined sources5
Turni981 – 983Combined sources3
Helixi987 – 999Combined sources13
Beta strandi1002 – 1004Combined sources3
Helixi1005 – 1015Combined sources11
Beta strandi1018 – 1020Combined sources3
Turni1022 – 1024Combined sources3
Helixi1025 – 1043Combined sources19
Helixi1045 – 1063Combined sources19
Beta strandi1069 – 1071Combined sources3
Beta strandi1077 – 1079Combined sources3
Helixi1112 – 1141Combined sources30
Beta strandi1147 – 1149Combined sources3
Beta strandi1152 – 1156Combined sources5
Helixi1157 – 1159Combined sources3
Helixi1160 – 1176Combined sources17
Helixi1179 – 1191Combined sources13
Helixi1198 – 1209Combined sources12
Helixi1219 – 1224Combined sources6
Beta strandi1228 – 1230Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3SPAX-ray2.50A105-1230[»]
4BOCX-ray2.65A151-1230[»]
ProteinModelPortaliO00411.
SMRiO00411.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni802 – 1230Mediates interaction with TEFM1 PublicationAdd BLAST429

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1038. Eukaryota.
COG5108. LUCA.
GeneTreeiENSGT00390000008060.
HOGENOMiHOG000044061.
HOVERGENiHBG078730.
InParanoidiO00411.
KOiK10908.
OMAiWFTECAR.
OrthoDBiEOG091G00YJ.
PhylomeDBiO00411.
TreeFamiTF105700.

Family and domain databases

Gene3Di1.10.1320.10. 1 hit.
1.10.287.260. 1 hit.
InterProiIPR024075. DNA-dir_RNA_pol_helix_hairpin.
IPR002092. DNA-dir_Rpol_phage-type.
IPR029262. RPOL_N.
[Graphical view]
PANTHERiPTHR10102. PTHR10102. 2 hits.
PfamiPF00940. RNA_pol. 1 hit.
PF14700. RPOL_N. 1 hit.
[Graphical view]
SMARTiSM01311. RPOL_N. 1 hit.
[Graphical view]
PROSITEiPS00900. RNA_POL_PHAGE_1. 1 hit.
PS00489. RNA_POL_PHAGE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O00411-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSALCWGRGA AGLKRALRPC GRPGLPGKEG TAGGVCGPRR SSSASPQEQD
60 70 80 90 100
QDRRKDWGHV ELLEVLQARV RQLQAESVSE VVVNRVDVAR LPECGSGDGS
110 120 130 140 150
LQPPRKVQMG AKDATPVPCG RWAKILEKDK RTQQMRMQRL KAKLQMPFQS
160 170 180 190 200
GEFKALTRRL QVEPRLLSKQ MAGCLEDCTR QAPESPWEEQ LARLLQEAPG
210 220 230 240 250
KLSLDVEQAP SGQHSQAQLS GQQQRLLAFF KCCLLTDQLP LAHHLLVVHH
260 270 280 290 300
GQRQKRKLLT LDMYNAVMLG WARQGAFKEL VYVLFMVKDA GLTPDLLSYA
310 320 330 340 350
AALQCMGRQD QDAGTIERCL EQMSQEGLKL QALFTAVLLS EEDRATVLKA
360 370 380 390 400
VHKVKPTFSL PPQLPPPVNT SKLLRDVYAK DGRVSYPKLH LPLKTLQCLF
410 420 430 440 450
EKQLHMELAS RVCVVSVEKP TLPSKEVKHA RKTLKTLRDQ WEKALCRALR
460 470 480 490 500
ETKNRLEREV YEGRFSLYPF LCLLDEREVV RMLLQVLQAL PAQGESFTTL
510 520 530 540 550
ARELSARTFS RHVVQRQRVS GQVQALQNHY RKYLCLLASD AEVPEPCLPR
560 570 580 590 600
QYWEELGAPE ALREQPWPLP VQMELGKLLA EMLVQATQMP CSLDKPHRSS
610 620 630 640 650
RLVPVLYHVY SFRNVQQIGI LKPHPAYVQL LEKAAEPTLT FEAVDVPMLC
660 670 680 690 700
PPLPWTSPHS GAFLLSPTKL MRTVEGATQH QELLETCPPT ALHGALDALT
710 720 730 740 750
QLGNCAWRVN GRVLDLVLQL FQAKGCPQLG VPAPPSEAPQ PPEAHLPHSA
760 770 780 790 800
APARKAELRR ELAHCQKVAR EMHSLRAEAL YRLSLAQHLR DRVFWLPHNM
810 820 830 840 850
DFRGRTYPCP PHFNHLGSDV ARALLEFAQG RPLGPHGLDW LKIHLVNLTG
860 870 880 890 900
LKKREPLRKR LAFAEEVMDD ILDSADQPLT GRKWWMGAEE PWQTLACCME
910 920 930 940 950
VANAVRASDP AAYVSHLPVH QDGSCNGLQH YAALGRDSVG AASVNLEPSD
960 970 980 990 1000
VPQDVYSGVA AQVEVFRRQD AQRGMRVAQV LEGFITRKVV KQTVMTVVYG
1010 1020 1030 1040 1050
VTRYGGRLQI EKRLRELSDF PQEFVWEASH YLVRQVFKSL QEMFSGTRAI
1060 1070 1080 1090 1100
QHWLTESARL ISHMGSVVEW VTPLGVPVIQ PYRLDSKVKQ IGGGIQSITY
1110 1120 1130 1140 1150
THNGDISRKP NTRKQKNGFP PNFIHSLDSS HMMLTALHCY RKGLTFVSVH
1160 1170 1180 1190 1200
DCYWTHAADV SVMNQVCREQ FVRLHSEPIL QDLSRFLVKR FCSEPQKILE
1210 1220 1230
ASQLKETLQA VPKPGAFDLE QVKRSTYFFS
Length:1,230
Mass (Da):138,620
Last modified:July 19, 2004 - v2
Checksum:i47AFBF2E0884AEFA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti399L → F in AAB58255 (PubMed:9097968).Curated1
Sequence conflicti983G → S in AAB58255 (PubMed:9097968).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_019427555E → A.Corresponds to variant rs2238549dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U75370 mRNA. Translation: AAB58255.1.
AC004449 Genomic DNA. Translation: AAC06147.1.
CCDSiCCDS12036.1.
RefSeqiNP_005026.3. NM_005035.3.
UniGeneiHs.254113.

Genome annotation databases

EnsembliENST00000588649; ENSP00000465759; ENSG00000099821.
GeneIDi5442.
KEGGihsa:5442.
UCSCiuc002lpf.2. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U75370 mRNA. Translation: AAB58255.1.
AC004449 Genomic DNA. Translation: AAC06147.1.
CCDSiCCDS12036.1.
RefSeqiNP_005026.3. NM_005035.3.
UniGeneiHs.254113.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3SPAX-ray2.50A105-1230[»]
4BOCX-ray2.65A151-1230[»]
ProteinModelPortaliO00411.
SMRiO00411.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111438. 34 interactors.
DIPiDIP-56412N.
IntActiO00411. 14 interactors.
MINTiMINT-1142772.
STRINGi9606.ENSP00000215591.

Chemistry databases

ChEMBLiCHEMBL3120041.

PTM databases

iPTMnetiO00411.
PhosphoSitePlusiO00411.

Polymorphism and mutation databases

BioMutaiPOLRMT.

Proteomic databases

EPDiO00411.
MaxQBiO00411.
PaxDbiO00411.
PeptideAtlasiO00411.
PRIDEiO00411.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000588649; ENSP00000465759; ENSG00000099821.
GeneIDi5442.
KEGGihsa:5442.
UCSCiuc002lpf.2. human.

Organism-specific databases

CTDi5442.
DisGeNETi5442.
GeneCardsiPOLRMT.
H-InvDBHIX0027511.
HIX0059707.
HGNCiHGNC:9200. POLRMT.
HPAiHPA006366.
MIMi601778. gene.
neXtProtiNX_O00411.
OpenTargetsiENSG00000099821.
PharmGKBiPA33522.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1038. Eukaryota.
COG5108. LUCA.
GeneTreeiENSGT00390000008060.
HOGENOMiHOG000044061.
HOVERGENiHBG078730.
InParanoidiO00411.
KOiK10908.
OMAiWFTECAR.
OrthoDBiEOG091G00YJ.
PhylomeDBiO00411.
TreeFamiTF105700.

Enzyme and pathway databases

BioCyciZFISH:HS01915-MONOMER.
ReactomeiR-HSA-163282. Mitochondrial transcription initiation.
R-HSA-2151201. Transcriptional activation of mitochondrial biogenesis.

Miscellaneous databases

GeneWikiiPOLRMT.
GenomeRNAii5442.
PROiO00411.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000099821.
CleanExiHS_POLRMT.
ExpressionAtlasiO00411. baseline and differential.
GenevisibleiO00411. HS.

Family and domain databases

Gene3Di1.10.1320.10. 1 hit.
1.10.287.260. 1 hit.
InterProiIPR024075. DNA-dir_RNA_pol_helix_hairpin.
IPR002092. DNA-dir_Rpol_phage-type.
IPR029262. RPOL_N.
[Graphical view]
PANTHERiPTHR10102. PTHR10102. 2 hits.
PfamiPF00940. RNA_pol. 1 hit.
PF14700. RPOL_N. 1 hit.
[Graphical view]
SMARTiSM01311. RPOL_N. 1 hit.
[Graphical view]
PROSITEiPS00900. RNA_POL_PHAGE_1. 1 hit.
PS00489. RNA_POL_PHAGE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRPOM_HUMAN
AccessioniPrimary (citable) accession number: O00411
Secondary accession number(s): O60370
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: July 19, 2004
Last modified: November 2, 2016
This is version 152 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.