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O00411 (RPOM_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 120. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA-directed RNA polymerase, mitochondrial
Short name=MtRPOL
EC=2.7.7.6
Gene names
Name:POLRMT
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length1230 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

DNA-dependent RNA polymerase catalyzes the transcription of mitochondrial DNA into RNA using the four ribonucleoside triphosphates as substrates. Ref.5

Catalytic activity

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

Subunit structure

Interacts with TFB1M and TFB2M, leading to the stimulation of transcription. Interacts with TEFM. Ref.3 Ref.5

Subcellular location

Mitochondrion.

Sequence similarities

Belongs to the phage and mitochondrial RNA polymerase family.

Ontologies

Keywords
   Biological processTranscription
   Cellular componentDNA-directed RNA polymerase
Mitochondrion
   Coding sequence diversityPolymorphism
   DomainTransit peptide
   Molecular functionNucleotidyltransferase
Transferase
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processtranscription initiation from mitochondrial promoter

Traceable author statement. Source: Reactome

   Cellular_componentmitochondrial nucleoid

Inferred from direct assay PubMed 18063578. Source: BHF-UCL

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: InterPro

DNA-directed RNA polymerase activity

Inferred from direct assay Ref.5. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 4141Mitochondrion Potential
Chain42 – 12301189DNA-directed RNA polymerase, mitochondrial
PRO_0000031068

Regions

Region802 – 1230429Mediates interaction with TEFM

Sites

Active site9221 By similarity
Active site9911 By similarity
Active site11511 By similarity

Natural variations

Natural variant5551E → A.
Corresponds to variant rs2238549 [ dbSNP | Ensembl ].
VAR_019427

Experimental info

Sequence conflict3991L → F in AAB58255. Ref.1
Sequence conflict9831G → S in AAB58255. Ref.1

Secondary structure

1 ........................................................................................................................................ 1230
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
O00411 [UniParc].

Last modified July 19, 2004. Version 2.
Checksum: 47AFBF2E0884AEFA

FASTA1,230138,620
        10         20         30         40         50         60 
MSALCWGRGA AGLKRALRPC GRPGLPGKEG TAGGVCGPRR SSSASPQEQD QDRRKDWGHV 

        70         80         90        100        110        120 
ELLEVLQARV RQLQAESVSE VVVNRVDVAR LPECGSGDGS LQPPRKVQMG AKDATPVPCG 

       130        140        150        160        170        180 
RWAKILEKDK RTQQMRMQRL KAKLQMPFQS GEFKALTRRL QVEPRLLSKQ MAGCLEDCTR 

       190        200        210        220        230        240 
QAPESPWEEQ LARLLQEAPG KLSLDVEQAP SGQHSQAQLS GQQQRLLAFF KCCLLTDQLP 

       250        260        270        280        290        300 
LAHHLLVVHH GQRQKRKLLT LDMYNAVMLG WARQGAFKEL VYVLFMVKDA GLTPDLLSYA 

       310        320        330        340        350        360 
AALQCMGRQD QDAGTIERCL EQMSQEGLKL QALFTAVLLS EEDRATVLKA VHKVKPTFSL 

       370        380        390        400        410        420 
PPQLPPPVNT SKLLRDVYAK DGRVSYPKLH LPLKTLQCLF EKQLHMELAS RVCVVSVEKP 

       430        440        450        460        470        480 
TLPSKEVKHA RKTLKTLRDQ WEKALCRALR ETKNRLEREV YEGRFSLYPF LCLLDEREVV 

       490        500        510        520        530        540 
RMLLQVLQAL PAQGESFTTL ARELSARTFS RHVVQRQRVS GQVQALQNHY RKYLCLLASD 

       550        560        570        580        590        600 
AEVPEPCLPR QYWEELGAPE ALREQPWPLP VQMELGKLLA EMLVQATQMP CSLDKPHRSS 

       610        620        630        640        650        660 
RLVPVLYHVY SFRNVQQIGI LKPHPAYVQL LEKAAEPTLT FEAVDVPMLC PPLPWTSPHS 

       670        680        690        700        710        720 
GAFLLSPTKL MRTVEGATQH QELLETCPPT ALHGALDALT QLGNCAWRVN GRVLDLVLQL 

       730        740        750        760        770        780 
FQAKGCPQLG VPAPPSEAPQ PPEAHLPHSA APARKAELRR ELAHCQKVAR EMHSLRAEAL 

       790        800        810        820        830        840 
YRLSLAQHLR DRVFWLPHNM DFRGRTYPCP PHFNHLGSDV ARALLEFAQG RPLGPHGLDW 

       850        860        870        880        890        900 
LKIHLVNLTG LKKREPLRKR LAFAEEVMDD ILDSADQPLT GRKWWMGAEE PWQTLACCME 

       910        920        930        940        950        960 
VANAVRASDP AAYVSHLPVH QDGSCNGLQH YAALGRDSVG AASVNLEPSD VPQDVYSGVA 

       970        980        990       1000       1010       1020 
AQVEVFRRQD AQRGMRVAQV LEGFITRKVV KQTVMTVVYG VTRYGGRLQI EKRLRELSDF 

      1030       1040       1050       1060       1070       1080 
PQEFVWEASH YLVRQVFKSL QEMFSGTRAI QHWLTESARL ISHMGSVVEW VTPLGVPVIQ 

      1090       1100       1110       1120       1130       1140 
PYRLDSKVKQ IGGGIQSITY THNGDISRKP NTRKQKNGFP PNFIHSLDSS HMMLTALHCY 

      1150       1160       1170       1180       1190       1200 
RKGLTFVSVH DCYWTHAADV SVMNQVCREQ FVRLHSEPIL QDLSRFLVKR FCSEPQKILE 

      1210       1220       1230 
ASQLKETLQA VPKPGAFDLE QVKRSTYFFS

« Hide

References

« Hide 'large scale' references
[1]"Identification of the gene encoding the human mitochondrial RNA polymerase (h-mtRPOL) by cyberscreening of the Expressed Sequence Tags database."
Tiranti V., Savoia A., Forti F., D'Apolito M.F., Centra M., Rocchi M., Zeviani M.
Hum. Mol. Genet. 6:615-625(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Liver.
[2]"The DNA sequence and biology of human chromosome 19."
Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. expand/collapse author list , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"Mitochondrial transcription factors B1 and B2 activate transcription of human mtDNA."
Falkenberg M., Gaspari M., Rantanen A., Trifunovic A., Larsson N.-G., Gustafsson C.M.
Nat. Genet. 31:289-294(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH TFB1M AND TFB2M.
[4]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[5]"TEFM (c17orf42) is necessary for transcription of human mtDNA."
Minczuk M., He J., Duch A.M., Ettema T.J., Chlebowski A., Dzionek K., Nijtmans L.G., Huynen M.A., Holt I.J.
Nucleic Acids Res. 39:4284-4299(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH TEFM.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U75370 mRNA. Translation: AAB58255.1.
AC004449 Genomic DNA. Translation: AAC06147.1.
IPIIPI00298738.
RefSeqNP_005026.3. NM_005035.3.
UniGeneHs.254113.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3SPAX-ray2.50A105-1230[»]
ProteinModelPortalO00411.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-56412N.
IntActO00411. 3 interactions.
MINTMINT-1142772.
STRING9606.ENSP00000215591.

PTM databases

PhosphoSiteO00411.

Proteomic databases

PaxDbO00411.
PRIDEO00411.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000215591; ENSP00000215591; ENSG00000099821.
GeneID5442.
KEGGhsa:5442.
UCSCuc002lpf.1. human.

Organism-specific databases

CTD5442.
GeneCardsGC19M000617.
H-InvDBHIX0027511.
HIX0059707.
HGNCHGNC:9200. POLRMT.
HPAHPA006366.
MIM601778. gene.
neXtProtNX_O00411.
PharmGKBPA33522.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG5108.
HOGENOMHOG000044061.
HOVERGENHBG078730.
InParanoidO00411.
KOK10908.
OMAWARKGSF.
PhylomeDBO00411.

Enzyme and pathway databases

ReactomeREACT_1788. Transcription.
REACT_71. Gene Expression.

Gene expression databases

BgeeO00411.
CleanExHS_POLRMT.
GenevestigatorO00411.
GermOnlineENSG00000099821. Homo sapiens.

Family and domain databases

Gene3D1.10.287.260. 1 hit.
InterProIPR024075. DNA-dir_RNA_pol_helix_hairpin.
IPR002092. DNA-dir_Rpol_phage-type.
[Graphical view]
PANTHERPTHR10102. PTHR10102. 1 hit.
PfamPF00940. RNA_pol. 1 hit.
[Graphical view]
PROSITEPS00900. RNA_POL_PHAGE_1. 1 hit.
PS00489. RNA_POL_PHAGE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi5442.
NextBio21059.
SOURCESearch...

Entry information

Entry nameRPOM_HUMAN
AccessionPrimary (citable) accession number: O00411
Secondary accession number(s): O60370
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: July 19, 2004
Last modified: May 1, 2013
This is version 120 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

Human chromosome 19

Human chromosome 19: entries, gene names and cross-references to MIM

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents