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O00411

- RPOM_HUMAN

UniProt

O00411 - RPOM_HUMAN

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Protein

DNA-directed RNA polymerase, mitochondrial

Gene
POLRMT
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of mitochondrial DNA into RNA using the four ribonucleoside triphosphates as substrates.1 Publication

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei922 – 9221 By similarity
Active sitei991 – 9911 By similarity
Active sitei1151 – 11511 By similarity

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. DNA-directed RNA polymerase activity Source: UniProtKB
  3. poly(A) RNA binding Source: UniProtKB
  4. protein binding Source: UniProtKB

GO - Biological processi

  1. gene expression Source: Reactome
  2. transcription from mitochondrial promoter Source: UniProtKB
  3. transcription initiation from mitochondrial promoter Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

Transcription

Enzyme and pathway databases

ReactomeiREACT_200608. Transcriptional activation of mitochondrial biogenesis.
REACT_367. Mitochondrial transcription initiation.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase, mitochondrial (EC:2.7.7.6)
Short name:
MtRPOL
Gene namesi
Name:POLRMT
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 19

Organism-specific databases

HGNCiHGNC:9200. POLRMT.

Subcellular locationi

GO - Cellular componenti

  1. mitochondrial matrix Source: Reactome
  2. mitochondrial nucleoid Source: BHF-UCL
  3. mitochondrion Source: HGNC
Complete GO annotation...

Keywords - Cellular componenti

DNA-directed RNA polymerase, Mitochondrion

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA33522.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 4141Mitochondrion Reviewed predictionAdd
BLAST
Chaini42 – 12301189DNA-directed RNA polymerase, mitochondrialPRO_0000031068Add
BLAST

Proteomic databases

MaxQBiO00411.
PaxDbiO00411.
PRIDEiO00411.

PTM databases

PhosphoSiteiO00411.

Expressioni

Gene expression databases

ArrayExpressiO00411.
BgeeiO00411.
CleanExiHS_POLRMT.
GenevestigatoriO00411.

Organism-specific databases

HPAiHPA006366.

Interactioni

Subunit structurei

Interacts with TFB1M and TFB2M, leading to the stimulation of transcription. Interacts with TEFM.2 Publications

Protein-protein interaction databases

BioGridi111438. 10 interactions.
DIPiDIP-56412N.
IntActiO00411. 4 interactions.
MINTiMINT-1142772.
STRINGi9606.ENSP00000215591.

Structurei

Secondary structure

1
1230
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi221 – 23616
Helixi239 – 25113
Helixi253 – 2564
Helixi261 – 27414
Helixi277 – 28913
Helixi296 – 30914
Helixi313 – 32513
Helixi331 – 3366
Helixi341 – 35111
Helixi352 – 3543
Turni372 – 3743
Helixi375 – 3784
Beta strandi380 – 3823
Helixi393 – 40917
Beta strandi411 – 4155
Helixi427 – 46135
Helixi469 – 4724
Helixi476 – 48914
Helixi497 – 51923
Helixi522 – 53312
Helixi534 – 5363
Helixi549 – 5579
Helixi562 – 5643
Helixi569 – 58618
Beta strandi588 – 5903
Beta strandi604 – 6107
Beta strandi613 – 6164
Beta strandi619 – 6235
Helixi625 – 63410
Beta strandi637 – 6426
Helixi643 – 6453
Beta strandi648 – 6503
Beta strandi653 – 6575
Beta strandi660 – 6667
Helixi677 – 6859
Helixi689 – 6924
Helixi693 – 70311
Beta strandi706 – 7094
Helixi711 – 72111
Turni722 – 7243
Helixi727 – 7293
Helixi735 – 7373
Beta strandi748 – 7503
Helixi772 – 78918
Beta strandi798 – 8003
Beta strandi806 – 8094
Beta strandi811 – 8133
Helixi819 – 8235
Beta strandi825 – 8295
Turni834 – 8363
Helixi837 – 84913
Helixi857 – 87620
Turni878 – 8803
Helixi884 – 8874
Beta strandi888 – 8903
Helixi891 – 90515
Beta strandi907 – 9093
Helixi910 – 9123
Beta strandi918 – 9225
Helixi926 – 9349
Helixi938 – 9436
Helixi955 – 97218
Helixi976 – 9805
Turni981 – 9833
Helixi987 – 99913
Beta strandi1002 – 10043
Helixi1005 – 101511
Beta strandi1018 – 10203
Turni1022 – 10243
Helixi1025 – 104319
Helixi1045 – 106319
Beta strandi1069 – 10713
Beta strandi1077 – 10793
Helixi1112 – 114130
Beta strandi1147 – 11493
Beta strandi1152 – 11565
Helixi1157 – 11593
Helixi1160 – 117617
Helixi1179 – 119113
Helixi1198 – 120912
Helixi1219 – 12246
Beta strandi1228 – 12303

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3SPAX-ray2.50A105-1230[»]
4BOCX-ray2.65A151-1230[»]
ProteinModelPortaliO00411.
SMRiO00411. Positions 218-1230.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni802 – 1230429Mediates interaction with TEFMAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG5108.
HOGENOMiHOG000044061.
HOVERGENiHBG078730.
InParanoidiO00411.
KOiK10908.
OMAiREMFTST.
PhylomeDBiO00411.
TreeFamiTF105700.

Family and domain databases

Gene3Di1.10.1320.10. 1 hit.
1.10.287.260. 1 hit.
InterProiIPR024075. DNA-dir_RNA_pol_helix_hairpin.
IPR002092. DNA-dir_Rpol_phage-type.
IPR029262. RPOL_N.
[Graphical view]
PANTHERiPTHR10102. PTHR10102. 1 hit.
PfamiPF00940. RNA_pol. 1 hit.
PF14700. RPOL_N. 1 hit.
[Graphical view]
PROSITEiPS00900. RNA_POL_PHAGE_1. 1 hit.
PS00489. RNA_POL_PHAGE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O00411-1 [UniParc]FASTAAdd to Basket

« Hide

MSALCWGRGA AGLKRALRPC GRPGLPGKEG TAGGVCGPRR SSSASPQEQD     50
QDRRKDWGHV ELLEVLQARV RQLQAESVSE VVVNRVDVAR LPECGSGDGS 100
LQPPRKVQMG AKDATPVPCG RWAKILEKDK RTQQMRMQRL KAKLQMPFQS 150
GEFKALTRRL QVEPRLLSKQ MAGCLEDCTR QAPESPWEEQ LARLLQEAPG 200
KLSLDVEQAP SGQHSQAQLS GQQQRLLAFF KCCLLTDQLP LAHHLLVVHH 250
GQRQKRKLLT LDMYNAVMLG WARQGAFKEL VYVLFMVKDA GLTPDLLSYA 300
AALQCMGRQD QDAGTIERCL EQMSQEGLKL QALFTAVLLS EEDRATVLKA 350
VHKVKPTFSL PPQLPPPVNT SKLLRDVYAK DGRVSYPKLH LPLKTLQCLF 400
EKQLHMELAS RVCVVSVEKP TLPSKEVKHA RKTLKTLRDQ WEKALCRALR 450
ETKNRLEREV YEGRFSLYPF LCLLDEREVV RMLLQVLQAL PAQGESFTTL 500
ARELSARTFS RHVVQRQRVS GQVQALQNHY RKYLCLLASD AEVPEPCLPR 550
QYWEELGAPE ALREQPWPLP VQMELGKLLA EMLVQATQMP CSLDKPHRSS 600
RLVPVLYHVY SFRNVQQIGI LKPHPAYVQL LEKAAEPTLT FEAVDVPMLC 650
PPLPWTSPHS GAFLLSPTKL MRTVEGATQH QELLETCPPT ALHGALDALT 700
QLGNCAWRVN GRVLDLVLQL FQAKGCPQLG VPAPPSEAPQ PPEAHLPHSA 750
APARKAELRR ELAHCQKVAR EMHSLRAEAL YRLSLAQHLR DRVFWLPHNM 800
DFRGRTYPCP PHFNHLGSDV ARALLEFAQG RPLGPHGLDW LKIHLVNLTG 850
LKKREPLRKR LAFAEEVMDD ILDSADQPLT GRKWWMGAEE PWQTLACCME 900
VANAVRASDP AAYVSHLPVH QDGSCNGLQH YAALGRDSVG AASVNLEPSD 950
VPQDVYSGVA AQVEVFRRQD AQRGMRVAQV LEGFITRKVV KQTVMTVVYG 1000
VTRYGGRLQI EKRLRELSDF PQEFVWEASH YLVRQVFKSL QEMFSGTRAI 1050
QHWLTESARL ISHMGSVVEW VTPLGVPVIQ PYRLDSKVKQ IGGGIQSITY 1100
THNGDISRKP NTRKQKNGFP PNFIHSLDSS HMMLTALHCY RKGLTFVSVH 1150
DCYWTHAADV SVMNQVCREQ FVRLHSEPIL QDLSRFLVKR FCSEPQKILE 1200
ASQLKETLQA VPKPGAFDLE QVKRSTYFFS 1230
Length:1,230
Mass (Da):138,620
Last modified:July 19, 2004 - v2
Checksum:i47AFBF2E0884AEFA
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti555 – 5551E → A.
Corresponds to variant rs2238549 [ dbSNP | Ensembl ].
VAR_019427

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti399 – 3991L → F in AAB58255. 1 Publication
Sequence conflicti983 – 9831G → S in AAB58255. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U75370 mRNA. Translation: AAB58255.1.
AC004449 Genomic DNA. Translation: AAC06147.1.
CCDSiCCDS12036.1.
RefSeqiNP_005026.3. NM_005035.3.
UniGeneiHs.254113.

Genome annotation databases

EnsembliENST00000588649; ENSP00000465759; ENSG00000099821.
GeneIDi5442.
KEGGihsa:5442.
UCSCiuc002lpf.1. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U75370 mRNA. Translation: AAB58255.1 .
AC004449 Genomic DNA. Translation: AAC06147.1 .
CCDSi CCDS12036.1.
RefSeqi NP_005026.3. NM_005035.3.
UniGenei Hs.254113.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3SPA X-ray 2.50 A 105-1230 [» ]
4BOC X-ray 2.65 A 151-1230 [» ]
ProteinModelPortali O00411.
SMRi O00411. Positions 218-1230.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 111438. 10 interactions.
DIPi DIP-56412N.
IntActi O00411. 4 interactions.
MINTi MINT-1142772.
STRINGi 9606.ENSP00000215591.

PTM databases

PhosphoSitei O00411.

Proteomic databases

MaxQBi O00411.
PaxDbi O00411.
PRIDEi O00411.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000588649 ; ENSP00000465759 ; ENSG00000099821 .
GeneIDi 5442.
KEGGi hsa:5442.
UCSCi uc002lpf.1. human.

Organism-specific databases

CTDi 5442.
GeneCardsi GC19M000617.
H-InvDB HIX0027511.
HIX0059707.
HGNCi HGNC:9200. POLRMT.
HPAi HPA006366.
MIMi 601778. gene.
neXtProti NX_O00411.
PharmGKBi PA33522.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG5108.
HOGENOMi HOG000044061.
HOVERGENi HBG078730.
InParanoidi O00411.
KOi K10908.
OMAi REMFTST.
PhylomeDBi O00411.
TreeFami TF105700.

Enzyme and pathway databases

Reactomei REACT_200608. Transcriptional activation of mitochondrial biogenesis.
REACT_367. Mitochondrial transcription initiation.

Miscellaneous databases

GeneWikii POLRMT.
GenomeRNAii 5442.
NextBioi 21059.
PROi O00411.
SOURCEi Search...

Gene expression databases

ArrayExpressi O00411.
Bgeei O00411.
CleanExi HS_POLRMT.
Genevestigatori O00411.

Family and domain databases

Gene3Di 1.10.1320.10. 1 hit.
1.10.287.260. 1 hit.
InterProi IPR024075. DNA-dir_RNA_pol_helix_hairpin.
IPR002092. DNA-dir_Rpol_phage-type.
IPR029262. RPOL_N.
[Graphical view ]
PANTHERi PTHR10102. PTHR10102. 1 hit.
Pfami PF00940. RNA_pol. 1 hit.
PF14700. RPOL_N. 1 hit.
[Graphical view ]
PROSITEi PS00900. RNA_POL_PHAGE_1. 1 hit.
PS00489. RNA_POL_PHAGE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of the gene encoding the human mitochondrial RNA polymerase (h-mtRPOL) by cyberscreening of the Expressed Sequence Tags database."
    Tiranti V., Savoia A., Forti F., D'Apolito M.F., Centra M., Rocchi M., Zeviani M.
    Hum. Mol. Genet. 6:615-625(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Liver.
  2. "The DNA sequence and biology of human chromosome 19."
    Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V.
    , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
    Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "Mitochondrial transcription factors B1 and B2 activate transcription of human mtDNA."
    Falkenberg M., Gaspari M., Rantanen A., Trifunovic A., Larsson N.-G., Gustafsson C.M.
    Nat. Genet. 31:289-294(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH TFB1M AND TFB2M.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  5. Cited for: FUNCTION, INTERACTION WITH TEFM.

Entry informationi

Entry nameiRPOM_HUMAN
AccessioniPrimary (citable) accession number: O00411
Secondary accession number(s): O60370
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: July 19, 2004
Last modified: September 3, 2014
This is version 131 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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