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Protein

Importin-5

Gene

IPO5

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (By similarity). Mediates the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding (BIB) domain of RPL23A. In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones. Binds to CPEB3 and mediates its nuclear import following neuronal stimulation (By similarity). In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev.By similarity1 Publication

GO - Molecular functioni

  • GTPase inhibitor activity Source: ProtInc
  • nuclear localization sequence binding Source: GO_Central
  • poly(A) RNA binding Source: UniProtKB
  • protein transporter activity Source: UniProtKB
  • Ran GTPase binding Source: ProtInc

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Host-virus interaction, Protein transport, Transport

Enzyme and pathway databases

BioCyciZFISH:ENSG00000065150-MONOMER.
ReactomeiR-HSA-192905. vRNP Assembly.

Protein family/group databases

TCDBi1.I.1.1.3. the nuclear pore complex (npc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Importin-5
Short name:
Imp5
Alternative name(s):
Importin subunit beta-3
Karyopherin beta-3
Ran-binding protein 5
Short name:
RanBP5
Gene namesi
Name:IPO5
Synonyms:KPNB3, RANBP5
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 13

Organism-specific databases

HGNCiHGNC:6402. IPO5.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • Golgi apparatus Source: HPA
  • intracellular membrane-bounded organelle Source: HPA
  • membrane Source: UniProtKB
  • nuclear membrane Source: HPA
  • nuclear periphery Source: GO_Central
  • nuclear pore Source: ProtInc
  • nucleolus Source: UniProtKB-SubCell
  • nucleoplasm Source: HPA
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi3843.
OpenTargetsiENSG00000065150.
PharmGKBiPA30193.

Polymorphism and mutation databases

BioMutaiIPO5.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources1 Publication
ChainiPRO_00001207712 – 1097Importin-5Add BLAST1096

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1 Publication1
Modified residuei827PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiO00410.
MaxQBiO00410.
PaxDbiO00410.
PeptideAtlasiO00410.
PRIDEiO00410.

PTM databases

iPTMnetiO00410.
PhosphoSitePlusiO00410.
SwissPalmiO00410.

Expressioni

Gene expression databases

BgeeiENSG00000065150.
CleanExiHS_IPO5.
ExpressionAtlasiO00410. baseline and differential.
GenevisibleiO00410. HS.

Organism-specific databases

HPAiCAB009110.
HPA040983.
HPA056548.

Interactioni

Subunit structurei

Binds RPL23A, RPS7 and RPL5 (PubMed:9687515). Interacts with H2A, H2B, H3 and H4 histones (By similarity). Interacts with CPEB3; this mediates CPEB3 nuclear import following neuronal stimulation which enhances the interaction in a RAN-regulated manner (PubMed:22730302). Binds to HIV-1 Rev (PubMed:16704975).By similarity3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
P279585EBI-356424,EBI-8753518From a different organism.
ACDQ96AP02EBI-356424,EBI-717666
GABARAPO951666EBI-356424,EBI-712001
GABARAPL1Q9H0R84EBI-356424,EBI-746969
GABARAPL2P605205EBI-356424,EBI-720116
MAP1LC3BQ9GZQ82EBI-356424,EBI-373144
MAP1LC3CQ9BXW42EBI-356424,EBI-2603996
RPL7P181244EBI-356424,EBI-350806

GO - Molecular functioni

  • Ran GTPase binding Source: ProtInc

Protein-protein interaction databases

BioGridi110041. 102 interactors.
DIPiDIP-41042N.
IntActiO00410. 42 interactors.
MINTiMINT-132356.
STRINGi9606.ENSP00000261574.

Structurei

3D structure databases

ProteinModelPortaliO00410.
SMRiO00410.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati5 – 38HEAT 1By similarity1 PublicationAdd BLAST34
Domaini28 – 99Importin N-terminalPROSITE-ProRule annotationAdd BLAST72
Repeati43 – 77HEAT 2By similarity1 PublicationAdd BLAST35
Repeati95 – 122HEAT 3By similarity1 PublicationAdd BLAST28
Repeati130 – 157HEAT 4By similarity1 PublicationAdd BLAST28
Repeati167 – 201HEAT 5By similarity1 PublicationAdd BLAST35
Repeati210 – 246HEAT 6By similarity1 PublicationAdd BLAST37
Repeati254 – 289HEAT 7By similarity1 PublicationAdd BLAST36
Repeati298 – 350HEAT 8By similarity1 PublicationAdd BLAST53
Repeati352 – 386HEAT 9By similarity1 PublicationAdd BLAST35
Repeati390 – 430HEAT 10By similarity1 PublicationAdd BLAST41
Repeati432 – 472HEAT 11By similarity1 PublicationAdd BLAST41
Repeati475 – 523HEAT 12By similarity1 PublicationAdd BLAST49
Repeati525 – 568HEAT 13By similarity1 PublicationAdd BLAST44
Repeati570 – 615HEAT 14By similarity1 PublicationAdd BLAST46
Repeati617 – 692HEAT 15By similarity1 PublicationAdd BLAST76
Repeati695 – 737HEAT 16By similarity1 PublicationAdd BLAST43
Repeati741 – 780HEAT 17By similarity1 PublicationAdd BLAST40
Repeati787 – 853HEAT 18By similarity1 PublicationAdd BLAST67
Repeati856 – 895HEAT 19By similarity1 PublicationAdd BLAST40
Repeati903 – 935HEAT 20By similarity1 PublicationAdd BLAST33
Repeati943 – 983HEAT 21By similarity1 PublicationAdd BLAST41
Repeati990 – 1021HEAT 22By similarity1 PublicationAdd BLAST32
Repeati1032 – 1067HEAT 23By similarity1 PublicationAdd BLAST36
Repeati1070 – 1093HEAT 24By similarity1 PublicationAdd BLAST24

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni325 – 375Ran-GTP bindingBy similarityAdd BLAST51

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi2 – 6Poly-Ala5
Compositional biasi460 – 465Poly-Ala6

Sequence similaritiesi

Contains 24 HEAT repeats.By similarity1 Publication
Contains 1 importin N-terminal domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG2171. Eukaryota.
ENOG410XQAJ. LUCA.
GeneTreeiENSGT00550000074773.
HOGENOMiHOG000209725.
HOVERGENiHBG006156.
InParanoidiO00410.
KOiK20222.
OMAiNLNNMQR.
OrthoDBiEOG091G011X.
PhylomeDBiO00410.
TreeFamiTF300344.

Family and domain databases

Gene3Di1.25.10.10. 3 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR000357. HEAT.
IPR001494. Importin-beta_N.
[Graphical view]
PfamiPF02985. HEAT. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 3 hits.
PROSITEiPS50166. IMPORTIN_B_NT. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O00410-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAAAAEQQQ FYLLLGNLLS PDNVVRKQAE ETYENIPGQS KITFLLQAIR
60 70 80 90 100
NTTAAEEARQ MAAVLLRRLL SSAFDEVYPA LPSDVQTAIK SELLMIIQME
110 120 130 140 150
TQSSMRKKVC DIAAELARNL IDEDGNNQWP EGLKFLFDSV SSQNVGLREA
160 170 180 190 200
ALHIFWNFPG IFGNQQQHYL DVIKRMLVQC MQDQEHPSIR TLSARATAAF
210 220 230 240 250
ILANEHNVAL FKHFADLLPG FLQAVNDSCY QNDDSVLKSL VEIADTVPKY
260 270 280 290 300
LRPHLEATLQ LSLKLCGDTS LNNMQRQLAL EVIVTLSETA AAMLRKHTNI
310 320 330 340 350
VAQTIPQMLA MMVDLEEDED WANADELEDD DFDSNAVAGE SALDRMACGL
360 370 380 390 400
GGKLVLPMIK EHIMQMLQNP DWKYRHAGLM ALSAIGEGCH QQMEGILNEI
410 420 430 440 450
VNFVLLFLQD PHPRVRYAAC NAVGQMATDF APGFQKKFHE KVIAALLQTM
460 470 480 490 500
EDQGNQRVQA HAAAALINFT EDCPKSLLIP YLDNLVKHLH SIMVLKLQEL
510 520 530 540 550
IQKGTKLVLE QVVTSIASVA DTAEEKFVPY YDLFMPSLKH IVENAVQKEL
560 570 580 590 600
RLLRGKTIEC ISLIGLAVGK EKFMQDASDV MQLLLKTQTD FNDMEDDDPQ
610 620 630 640 650
ISYMISAWAR MCKILGKEFQ QYLPVVMGPL MKTASIKPEV ALLDTQDMEN
660 670 680 690 700
MSDDDGWEFV NLGDQQSFGI KTAGLEEKST ACQMLVCYAK ELKEGFVEYT
710 720 730 740 750
EQVVKLMVPL LKFYFHDGVR VAAAESMPLL LECARVRGPE YLTQMWHFMC
760 770 780 790 800
DALIKAIGTE PDSDVLSEIM HSFAKCIEVM GDGCLNNEHF EELGGILKAK
810 820 830 840 850
LEEHFKNQEL RQVKRQDEDY DEQVEESLQD EDDNDVYILT KVSDILHSIF
860 870 880 890 900
SSYKEKVLPW FEQLLPLIVN LICPHRPWPD RQWGLCIFDD VIEHCSPASF
910 920 930 940 950
KYAEYFLRPM LQYVCDNSPE VRQAAAYGLG VMAQYGGDNY RPFCTEALPL
960 970 980 990 1000
LVRVIQSADS KTKENVNATE NCISAVGKIM KFKPDCVNVE EVLPHWLSWL
1010 1020 1030 1040 1050
PLHEDKEEAV QTFNYLCDLI ESNHPIVLGP NNTNLPKIFS IIAEGEMHEA
1060 1070 1080 1090
IKHEDPCAKR LANVVRQVQT SGGLWTECIA QLSPEQQAAI QELLNSA
Length:1,097
Mass (Da):123,630
Last modified:January 23, 2007 - v4
Checksum:i1864AD23513F2DE1
GO
Isoform 2 (identifier: O00410-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-60: Missing.

Show »
Length:1,037
Mass (Da):116,982
Checksum:i7D9A925F7DBC1DE0
GO
Isoform 3 (identifier: O00410-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MPEDQVGKLEATENTISAM

Show »
Length:1,115
Mass (Da):125,545
Checksum:i5296EF23A427A0FB
GO

Sequence cautioni

The sequence CAA70103 differs from that shown. Reason: Frameshift at position 18.Curated
Isoform 3 : The sequence CAA70103 differs from that shown. Reason: Frameshift at position 18.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti538L → R in AAH45640 (PubMed:15489334).Curated1
Sequence conflicti826 – 827ES → GT in AAC51317 (PubMed:9114010).Curated2

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_012029286L → I.Corresponds to variant rs1053814dbSNPEnsembl.1
Natural variantiVAR_012030525E → K.Corresponds to variant rs632729dbSNPEnsembl.1
Natural variantiVAR_012031549E → K.Corresponds to variant rs484770dbSNPEnsembl.1
Natural variantiVAR_012032905Y → C.Corresponds to variant rs1804740dbSNPEnsembl.1
Natural variantiVAR_012033969T → I.Corresponds to variant rs1804741dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0375871 – 60Missing in isoform 2. 1 PublicationAdd BLAST60
Alternative sequenceiVSP_0377741M → MPEDQVGKLEATENTISAM in isoform 3. 2 Publications1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U72761 mRNA. Translation: AAC51317.1.
Y08890 mRNA. Translation: CAA70103.1. Frameshift.
AK302812 mRNA. Translation: BAG64012.1.
AL356580, AL137120 Genomic DNA. Translation: CAI13757.1.
AL137120, AL356580 Genomic DNA. Translation: CAI16520.1.
CH471085 Genomic DNA. Translation: EAX08980.1.
BC001497 mRNA. Translation: AAH01497.1.
BC019309 mRNA. Translation: AAH19309.1.
BC045640 mRNA. Translation: AAH45640.1.
CCDSiCCDS31999.1. [O00410-3]
RefSeqiNP_002262.3. NM_002271.4. [O00410-3]
XP_005254109.1. XM_005254052.3. [O00410-3]
XP_005254110.1. XM_005254053.3. [O00410-3]
XP_011519389.1. XM_011521087.2. [O00410-3]
XP_011519390.1. XM_011521088.2. [O00410-3]
XP_011519391.1. XM_011521089.2. [O00410-3]
XP_011519392.1. XM_011521090.2. [O00410-3]
XP_016876051.1. XM_017020562.1. [O00410-3]
UniGeneiHs.712598.

Genome annotation databases

EnsembliENST00000261574; ENSP00000261574; ENSG00000065150. [O00410-3]
ENST00000357602; ENSP00000350219; ENSG00000065150. [O00410-1]
ENST00000490680; ENSP00000418393; ENSG00000065150. [O00410-1]
GeneIDi3843.
KEGGihsa:3843.
UCSCiuc001vne.4. human. [O00410-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U72761 mRNA. Translation: AAC51317.1.
Y08890 mRNA. Translation: CAA70103.1. Frameshift.
AK302812 mRNA. Translation: BAG64012.1.
AL356580, AL137120 Genomic DNA. Translation: CAI13757.1.
AL137120, AL356580 Genomic DNA. Translation: CAI16520.1.
CH471085 Genomic DNA. Translation: EAX08980.1.
BC001497 mRNA. Translation: AAH01497.1.
BC019309 mRNA. Translation: AAH19309.1.
BC045640 mRNA. Translation: AAH45640.1.
CCDSiCCDS31999.1. [O00410-3]
RefSeqiNP_002262.3. NM_002271.4. [O00410-3]
XP_005254109.1. XM_005254052.3. [O00410-3]
XP_005254110.1. XM_005254053.3. [O00410-3]
XP_011519389.1. XM_011521087.2. [O00410-3]
XP_011519390.1. XM_011521088.2. [O00410-3]
XP_011519391.1. XM_011521089.2. [O00410-3]
XP_011519392.1. XM_011521090.2. [O00410-3]
XP_016876051.1. XM_017020562.1. [O00410-3]
UniGeneiHs.712598.

3D structure databases

ProteinModelPortaliO00410.
SMRiO00410.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi110041. 102 interactors.
DIPiDIP-41042N.
IntActiO00410. 42 interactors.
MINTiMINT-132356.
STRINGi9606.ENSP00000261574.

Protein family/group databases

TCDBi1.I.1.1.3. the nuclear pore complex (npc) family.

PTM databases

iPTMnetiO00410.
PhosphoSitePlusiO00410.
SwissPalmiO00410.

Polymorphism and mutation databases

BioMutaiIPO5.

Proteomic databases

EPDiO00410.
MaxQBiO00410.
PaxDbiO00410.
PeptideAtlasiO00410.
PRIDEiO00410.

Protocols and materials databases

DNASUi3843.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000261574; ENSP00000261574; ENSG00000065150. [O00410-3]
ENST00000357602; ENSP00000350219; ENSG00000065150. [O00410-1]
ENST00000490680; ENSP00000418393; ENSG00000065150. [O00410-1]
GeneIDi3843.
KEGGihsa:3843.
UCSCiuc001vne.4. human. [O00410-1]

Organism-specific databases

CTDi3843.
DisGeNETi3843.
GeneCardsiIPO5.
H-InvDBHIX0174410.
HGNCiHGNC:6402. IPO5.
HPAiCAB009110.
HPA040983.
HPA056548.
MIMi602008. gene.
neXtProtiNX_O00410.
OpenTargetsiENSG00000065150.
PharmGKBiPA30193.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2171. Eukaryota.
ENOG410XQAJ. LUCA.
GeneTreeiENSGT00550000074773.
HOGENOMiHOG000209725.
HOVERGENiHBG006156.
InParanoidiO00410.
KOiK20222.
OMAiNLNNMQR.
OrthoDBiEOG091G011X.
PhylomeDBiO00410.
TreeFamiTF300344.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000065150-MONOMER.
ReactomeiR-HSA-192905. vRNP Assembly.

Miscellaneous databases

ChiTaRSiIPO5. human.
GeneWikiiRANBP5.
GenomeRNAii3843.
PROiO00410.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000065150.
CleanExiHS_IPO5.
ExpressionAtlasiO00410. baseline and differential.
GenevisibleiO00410. HS.

Family and domain databases

Gene3Di1.25.10.10. 3 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR000357. HEAT.
IPR001494. Importin-beta_N.
[Graphical view]
PfamiPF02985. HEAT. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 3 hits.
PROSITEiPS50166. IMPORTIN_B_NT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIPO5_HUMAN
AccessioniPrimary (citable) accession number: O00410
Secondary accession number(s): B4DZA0
, O15257, Q5T578, Q86XC7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 166 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 13
    Human chromosome 13: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.