O00409 (FOXN3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Forkhead box protein N3

Alternative name(s):
Checkpoint suppressor 1

Gene names

Name:	FOXN3
Synonyms:	C14orf116, CHES1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	490 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts as a transcriptional repressor. May be involved in DNA damage-inducible cell cycle arrests (checkpoints). Ref.4
Subunit structure	Interacts through its C-terminus with the C-terminus of SNW1/SKIP. Ref.4
Subcellular location	Nucleus Probable.
Sequence similarities	Contains 1 fork-head DNA-binding domain.

Ontologies

Keywords
Biological process	Cell cycle Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	DNA-binding
Molecular function	Repressor
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA damage checkpoint Traceable author statement Ref.1. Source: ProtInc G2 phase of mitotic cell cycle Traceable author statement Ref.1. Source: ProtInc embryo development Inferred from Biological aspect of Ancestor. Source: RefGenome negative regulation of transcription, DNA-dependent Inferred from direct assay Ref.4. Source: UniProtKB pattern specification process Inferred from Biological aspect of Ancestor. Source: RefGenome regulation of sequence-specific DNA binding transcription factor activity Inferred from Biological aspect of Ancestor. Source: RefGenome tissue development Inferred from Biological aspect of Ancestor. Source: RefGenome
Cellular_component	transcription factor complex Inferred from Biological aspect of Ancestor. Source: RefGenome
Molecular_function	DNA binding, bending Inferred from Biological aspect of Ancestor. Source: RefGenome RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity Inferred from Biological aspect of Ancestor. Source: RefGenome double-stranded DNA binding Inferred from Biological aspect of Ancestor. Source: RefGenome sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro transcription factor binding Inferred from Biological aspect of Ancestor. Source: RefGenome
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
SNW1	Q13573	3	EBI-372721,EBI-632715

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O00409-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O00409-2)

The sequence of this isoform differs from the canonical sequence as follows:
     249-271: DPDIDAASAMMLLNTPPEIQAGF → V

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 490	490	Forkhead box protein N3		PRO_0000091869
Regions
	DNA binding	113 – 204	92	Fork-head
Amino acid modifications
	Modified residue	85	1	Phosphoserine Ref.5
Natural variations
	Alternative sequence	249 – 271	23	DPDID…IQAGF → V in isoform 2.		VSP_001551
	Natural variant	337	1	Y → H. Corresponds to variant rs1804717 [ dbSNP | Ensembl ].		VAR_049163

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: EE07975A592C8C01	FASTA	490	53,835
	10 20 30 40 50 60 MGPVMPPSKK PESSGISVSS GLSQCYGGSG FSKALQEDDD LDFSLPDIRL EEGAMEDEEL 70 80 90 100 110 120 TNLNWLHESK NLLKSFGESV LRSVSPVQDL DDDTPPSPAH SDMPYDARQN PNCKPPYSFS 130 140 150 160 170 180 CLIFMAIEDS PTKRLPVKDI YNWILEHFPY FANAPTGWKN SVRHNLSLNK CFKKVDKERS 190 200 210 220 230 240 QSIGKGSLWC IDPEYRQNLI QALKKTPYHP HPHVFNTPPT CPQAYQSTSG PPIWPGSTFF 250 260 270 280 290 300 KRNGALLQDP DIDAASAMML LNTPPEIQAG FPPGVIQNGA RVLSRGLFPG VRPLPITPIG 310 320 330 340 350 360 VTAAMRNGIT SCRMRTESEP SCGSPVVSGD PKEDHNYSSA KSSNARSTSP TSDSISSSSS 370 380 390 400 410 420 SADDHYEFAT KGSQEGSEGS EGSFRSHESP SDTEEDDRKH SQKEPKDSLG DSGYASQHKK 430 440 450 460 470 480 RQHFAKARKV PSDTLPLKKR RTEKPPESDD EEMKEAAGSL LHLAGIRSCL NNITNRTAKG 490 QKEQKETTKN
	Isoform 2 [UniParc]. Checksum: FE3B5805FE885680	FASTA	468	51,534
	10 20 30 40 50 60 MGPVMPPSKK PESSGISVSS GLSQCYGGSG FSKALQEDDD LDFSLPDIRL EEGAMEDEEL 70 80 90 100 110 120 TNLNWLHESK NLLKSFGESV LRSVSPVQDL DDDTPPSPAH SDMPYDARQN PNCKPPYSFS 130 140 150 160 170 180 CLIFMAIEDS PTKRLPVKDI YNWILEHFPY FANAPTGWKN SVRHNLSLNK CFKKVDKERS 190 200 210 220 230 240 QSIGKGSLWC IDPEYRQNLI QALKKTPYHP HPHVFNTPPT CPQAYQSTSG PPIWPGSTFF 250 260 270 280 290 300 KRNGALLQVP PGVIQNGARV LSRGLFPGVR PLPITPIGVT AAMRNGITSC RMRTESEPSC 310 320 330 340 350 360 GSPVVSGDPK EDHNYSSAKS SNARSTSPTS DSISSSSSSA DDHYEFATKG SQEGSEGSEG 370 380 390 400 410 420 SFRSHESPSD TEEDDRKHSQ KEPKDSLGDS GYASQHKKRQ HFAKARKVPS DTLPLKKRRT 430 440 450 460 EKPPESDDEE MKEAAGSLLH LAGIRSCLNN ITNRTAKGQK EQKETTKN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Reconstitution of a MEC1-independent checkpoint in yeast by expression of a novel human fork head cDNA." Pati D., Keller C., Groudine M., Plon S.E. Mol. Cell. Biol. 17:3037-3046(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"The DNA sequence and analysis of human chromosome 14." Heilig R., Eckenberg R., Petit J.-L., Fonknechten N., Da Silva C., Cattolico L., Levy M., Barbe V., De Berardinis V., Ureta-Vidal A., Pelletier E., Vico V., Anthouard V., Rowen L., Madan A., Qin S., Sun H., Du H. , Pepin K., Artiguenave F., Robert C., Cruaud C., Bruels T., Jaillon O., Friedlander L., Samson G., Brottier P., Cure S., Segurens B., Aniere F., Samain S., Crespeau H., Abbasi N., Aiach N., Boscus D., Dickhoff R., Dors M., Dubois I., Friedman C., Gouyvenoux M., James R., Madan A., Mairey-Estrada B., Mangenot S., Martins N., Menard M., Oztas S., Ratcliffe A., Shaffer T., Trask B., Vacherie B., Bellemere C., Belser C., Besnard-Gonnet M., Bartol-Mavel D., Boutard M., Briez-Silla S., Combette S., Dufosse-Laurent V., Ferron C., Lechaplais C., Louesse C., Muselet D., Magdelenat G., Pateau E., Petit E., Sirvain-Trukniewicz P., Trybou A., Vega-Czarny N., Bataille E., Bluet E., Bordelais I., Dubois M., Dumont C., Guerin T., Haffray S., Hammadi R., Muanga J., Pellouin V., Robert D., Wunderle E., Gauguet G., Roy A., Sainte-Marthe L., Verdier J., Verdier-Discala C., Hillier L.W., Fulton L., McPherson J., Matsuda F., Wilson R., Scarpelli C., Gyapay G., Wincker P., Saurin W., Quetier F., Waterston R., Hood L., Weissenbach J. Nature 421:601-607(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Muscle.
[4]	"CHES1/FOXN3 interacts with Ski-interacting protein and acts as a transcriptional repressor." Scott K.L., Plon S.E. Gene 359:119-126(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH SNW1.
[5]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-85, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[6]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U68723 mRNA. Translation: AAB58252.1. AC007263 Genomic DNA. Translation: AAF18259.1. AL138478 Genomic DNA. No translation available. AL356805 Genomic DNA. No translation available. AL357093 Genomic DNA. No translation available. BC007506 mRNA. Translation: AAH07506.1.
IPI	IPI00011681. IPI00220563.
RefSeq	NP_001078940.1. NM_001085471.1. NP_005188.2. NM_005197.3.
UniGene	Hs.434286. Hs.621371.
3D structure databases
ProteinModelPortal	O00409.
ModBase	Search...
Protein-protein interaction databases
IntAct	O00409. 2 interactions.
STRING	9606.ENSP00000261302.
PTM databases
PhosphoSite	O00409.
Proteomic databases
PaxDb	O00409.
PRIDE	O00409.
Protocols and materials databases
DNASU	1112.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000261302; ENSP00000261302; ENSG00000053254. ENST00000345097; ENSP00000343288; ENSG00000053254. ENST00000555353; ENSP00000452227; ENSG00000053254. ENST00000557258; ENSP00000452005; ENSG00000053254.
GeneID	1112.
KEGG	hsa:1112.
UCSC	uc001xxn.4. human. uc001xxo.4. human.
Organism-specific databases
CTD	1112.
GeneCards	GC14M089622.
HGNC	HGNC:1928. FOXN3.
MIM	602628. gene.
neXtProt	NX_O00409.
PharmGKB	PA26460.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG5025.
HOGENOM	HOG000095166.
HOVERGEN	HBG051654.
InParanoid	O00409.
KO	K09407.
OMA	MRTESEQ.
OrthoDB	EOG4N30NV.
PhylomeDB	O00409.
Gene expression databases
ArrayExpress	O00409.
Bgee	O00409.
CleanEx	HS_FOXN3.
Genevestigator	O00409.
GermOnline	ENSG00000053254. Homo sapiens.
Family and domain databases
Gene3D	1.10.10.10. 1 hit.
InterPro	IPR001766. TF_fork_head. IPR018122. TF_fork_head_CS. IPR011991. WHTH_DNA-bd_dom. [Graphical view]
Pfam	PF00250. Fork_head. 1 hit. [Graphical view]
PRINTS	PR00053. FORKHEAD.
SMART	SM00339. FH. 1 hit. [Graphical view]
PROSITE	PS00657. FORK_HEAD_1. 1 hit. PS00658. FORK_HEAD_2. 1 hit. PS50039. FORK_HEAD_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	FOXN3. human.
GenomeRNAi	1112.
NextBio	4612.
SOURCE	Search...

Entry information

Entry name

FOXN3_HUMAN

Accession

Primary (citable) accession number: O00409
Secondary accession number(s): Q96II7, Q9UIE7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	July 1, 1997
Last modified:	May 1, 2013
This is version 129 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.