Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Forkhead box protein N3

Gene

FOXN3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a transcriptional repressor. May be involved in DNA damage-inducible cell cycle arrests (checkpoints).1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi113 – 20492Fork-headPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. protein C-terminus binding Source: UniProtKB
  2. sequence-specific DNA binding Source: InterPro
  3. sequence-specific DNA binding transcription factor activity Source: InterPro

GO - Biological processi

  1. mitotic G2 DNA damage checkpoint Source: UniProtKB
  2. negative regulation of transcription, DNA-templated Source: UniProtKB
  3. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Cell cycle, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Forkhead box protein N3
Alternative name(s):
Checkpoint suppressor 1
Gene namesi
Name:FOXN3
Synonyms:C14orf116, CHES1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 14

Organism-specific databases

HGNCiHGNC:1928. FOXN3.

Subcellular locationi

Nucleus Curated

GO - Cellular componenti

  1. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA26460.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 490490Forkhead box protein N3PRO_0000091869Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei85 – 851Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiO00409.
PaxDbiO00409.
PRIDEiO00409.

PTM databases

PhosphoSiteiO00409.

Expressioni

Gene expression databases

BgeeiO00409.
CleanExiHS_FOXN3.
ExpressionAtlasiO00409. baseline and differential.
GenevestigatoriO00409.

Organism-specific databases

HPAiHPA059209.

Interactioni

Subunit structurei

Interacts through its C-terminus with the C-terminus of SNW1/SKIP.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
SNW1Q135733EBI-372721,EBI-632715

Protein-protein interaction databases

BioGridi107537. 4 interactions.
IntActiO00409. 3 interactions.
STRINGi9606.ENSP00000261302.

Structurei

3D structure databases

ProteinModelPortaliO00409.
SMRiO00409. Positions 114-194.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Contains 1 fork-head DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5025.
GeneTreeiENSGT00780000121868.
HOGENOMiHOG000095166.
HOVERGENiHBG051654.
InParanoidiO00409.
KOiK09407.
OMAiMRTESEQ.
PhylomeDBiO00409.
TreeFamiTF105083.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR001766. TF_fork_head.
IPR018122. TF_fork_head_CS_1.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00250. Fork_head. 1 hit.
[Graphical view]
PRINTSiPR00053. FORKHEAD.
SMARTiSM00339. FH. 1 hit.
[Graphical view]
PROSITEiPS00657. FORK_HEAD_1. 1 hit.
PS00658. FORK_HEAD_2. 1 hit.
PS50039. FORK_HEAD_3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O00409-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGPVMPPSKK PESSGISVSS GLSQCYGGSG FSKALQEDDD LDFSLPDIRL
60 70 80 90 100
EEGAMEDEEL TNLNWLHESK NLLKSFGESV LRSVSPVQDL DDDTPPSPAH
110 120 130 140 150
SDMPYDARQN PNCKPPYSFS CLIFMAIEDS PTKRLPVKDI YNWILEHFPY
160 170 180 190 200
FANAPTGWKN SVRHNLSLNK CFKKVDKERS QSIGKGSLWC IDPEYRQNLI
210 220 230 240 250
QALKKTPYHP HPHVFNTPPT CPQAYQSTSG PPIWPGSTFF KRNGALLQDP
260 270 280 290 300
DIDAASAMML LNTPPEIQAG FPPGVIQNGA RVLSRGLFPG VRPLPITPIG
310 320 330 340 350
VTAAMRNGIT SCRMRTESEP SCGSPVVSGD PKEDHNYSSA KSSNARSTSP
360 370 380 390 400
TSDSISSSSS SADDHYEFAT KGSQEGSEGS EGSFRSHESP SDTEEDDRKH
410 420 430 440 450
SQKEPKDSLG DSGYASQHKK RQHFAKARKV PSDTLPLKKR RTEKPPESDD
460 470 480 490
EEMKEAAGSL LHLAGIRSCL NNITNRTAKG QKEQKETTKN
Length:490
Mass (Da):53,835
Last modified:July 1, 1997 - v1
Checksum:iEE07975A592C8C01
GO
Isoform 2 (identifier: O00409-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     249-271: DPDIDAASAMMLLNTPPEIQAGF → V

Note: No experimental confirmation available.

Show »
Length:468
Mass (Da):51,534
Checksum:iFE3B5805FE885680
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti337 – 3371Y → H.
Corresponds to variant rs1804717 [ dbSNP | Ensembl ].
VAR_049163

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei249 – 27123DPDID…IQAGF → V in isoform 2. 1 PublicationVSP_001551Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U68723 mRNA. Translation: AAB58252.1.
AC007263 Genomic DNA. Translation: AAF18259.1.
AL138478 Genomic DNA. No translation available.
AL356805 Genomic DNA. No translation available.
AL357093 Genomic DNA. No translation available.
BC007506 mRNA. Translation: AAH07506.1.
CCDSiCCDS32138.1. [O00409-2]
CCDS41977.1. [O00409-1]
RefSeqiNP_001078940.1. NM_001085471.1. [O00409-1]
NP_005188.2. NM_005197.3. [O00409-2]
UniGeneiHs.434286.

Genome annotation databases

EnsembliENST00000261302; ENSP00000261302; ENSG00000053254. [O00409-1]
ENST00000345097; ENSP00000343288; ENSG00000053254. [O00409-1]
ENST00000555353; ENSP00000452227; ENSG00000053254. [O00409-2]
ENST00000557258; ENSP00000452005; ENSG00000053254. [O00409-2]
ENST00000615335; ENSP00000479114; ENSG00000053254. [O00409-2]
GeneIDi1112.
KEGGihsa:1112.
UCSCiuc001xxn.4. human. [O00409-2]
uc001xxo.4. human. [O00409-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U68723 mRNA. Translation: AAB58252.1.
AC007263 Genomic DNA. Translation: AAF18259.1.
AL138478 Genomic DNA. No translation available.
AL356805 Genomic DNA. No translation available.
AL357093 Genomic DNA. No translation available.
BC007506 mRNA. Translation: AAH07506.1.
CCDSiCCDS32138.1. [O00409-2]
CCDS41977.1. [O00409-1]
RefSeqiNP_001078940.1. NM_001085471.1. [O00409-1]
NP_005188.2. NM_005197.3. [O00409-2]
UniGeneiHs.434286.

3D structure databases

ProteinModelPortaliO00409.
SMRiO00409. Positions 114-194.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi107537. 4 interactions.
IntActiO00409. 3 interactions.
STRINGi9606.ENSP00000261302.

PTM databases

PhosphoSiteiO00409.

Proteomic databases

MaxQBiO00409.
PaxDbiO00409.
PRIDEiO00409.

Protocols and materials databases

DNASUi1112.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000261302; ENSP00000261302; ENSG00000053254. [O00409-1]
ENST00000345097; ENSP00000343288; ENSG00000053254. [O00409-1]
ENST00000555353; ENSP00000452227; ENSG00000053254. [O00409-2]
ENST00000557258; ENSP00000452005; ENSG00000053254. [O00409-2]
ENST00000615335; ENSP00000479114; ENSG00000053254. [O00409-2]
GeneIDi1112.
KEGGihsa:1112.
UCSCiuc001xxn.4. human. [O00409-2]
uc001xxo.4. human. [O00409-1]

Organism-specific databases

CTDi1112.
GeneCardsiGC14M090293.
HGNCiHGNC:1928. FOXN3.
HPAiHPA059209.
MIMi602628. gene.
neXtProtiNX_O00409.
PharmGKBiPA26460.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG5025.
GeneTreeiENSGT00780000121868.
HOGENOMiHOG000095166.
HOVERGENiHBG051654.
InParanoidiO00409.
KOiK09407.
OMAiMRTESEQ.
PhylomeDBiO00409.
TreeFamiTF105083.

Miscellaneous databases

ChiTaRSiFOXN3. human.
GeneWikiiFOXN3.
GenomeRNAii1112.
NextBioi4612.
PROiO00409.
SOURCEiSearch...

Gene expression databases

BgeeiO00409.
CleanExiHS_FOXN3.
ExpressionAtlasiO00409. baseline and differential.
GenevestigatoriO00409.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR001766. TF_fork_head.
IPR018122. TF_fork_head_CS_1.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00250. Fork_head. 1 hit.
[Graphical view]
PRINTSiPR00053. FORKHEAD.
SMARTiSM00339. FH. 1 hit.
[Graphical view]
PROSITEiPS00657. FORK_HEAD_1. 1 hit.
PS00658. FORK_HEAD_2. 1 hit.
PS50039. FORK_HEAD_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Reconstitution of a MEC1-independent checkpoint in yeast by expression of a novel human fork head cDNA."
    Pati D., Keller C., Groudine M., Plon S.E.
    Mol. Cell. Biol. 17:3037-3046(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  2. "The DNA sequence and analysis of human chromosome 14."
    Heilig R., Eckenberg R., Petit J.-L., Fonknechten N., Da Silva C., Cattolico L., Levy M., Barbe V., De Berardinis V., Ureta-Vidal A., Pelletier E., Vico V., Anthouard V., Rowen L., Madan A., Qin S., Sun H., Du H.
    , Pepin K., Artiguenave F., Robert C., Cruaud C., Bruels T., Jaillon O., Friedlander L., Samson G., Brottier P., Cure S., Segurens B., Aniere F., Samain S., Crespeau H., Abbasi N., Aiach N., Boscus D., Dickhoff R., Dors M., Dubois I., Friedman C., Gouyvenoux M., James R., Madan A., Mairey-Estrada B., Mangenot S., Martins N., Menard M., Oztas S., Ratcliffe A., Shaffer T., Trask B., Vacherie B., Bellemere C., Belser C., Besnard-Gonnet M., Bartol-Mavel D., Boutard M., Briez-Silla S., Combette S., Dufosse-Laurent V., Ferron C., Lechaplais C., Louesse C., Muselet D., Magdelenat G., Pateau E., Petit E., Sirvain-Trukniewicz P., Trybou A., Vega-Czarny N., Bataille E., Bluet E., Bordelais I., Dubois M., Dumont C., Guerin T., Haffray S., Hammadi R., Muanga J., Pellouin V., Robert D., Wunderle E., Gauguet G., Roy A., Sainte-Marthe L., Verdier J., Verdier-Discala C., Hillier L.W., Fulton L., McPherson J., Matsuda F., Wilson R., Scarpelli C., Gyapay G., Wincker P., Saurin W., Quetier F., Waterston R., Hood L., Weissenbach J.
    Nature 421:601-607(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Muscle.
  4. "CHES1/FOXN3 interacts with Ski-interacting protein and acts as a transcriptional repressor."
    Scott K.L., Plon S.E.
    Gene 359:119-126(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH SNW1.
  5. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
    Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
    Sci. Signal. 2:RA46-RA46(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-85, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Leukemic T-cell.
  6. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
    Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
    Sci. Signal. 4:RS3-RS3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiFOXN3_HUMAN
AccessioniPrimary (citable) accession number: O00409
Secondary accession number(s): Q96II7, Q9UIE7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: July 1, 1997
Last modified: April 1, 2015
This is version 149 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.