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Protein

Neural Wiskott-Aldrich syndrome protein

Gene

WASL

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulates actin polymerization by stimulating the actin-nucleating activity of the Arp2/3 complex. Involved in mitosis and cytokinesis, via its role in the regulation of actin polymerization. Binds to HSF1/HSTF1 and forms a complex on heat shock promoter elements (HSE) that negatively regulates HSP90 expression.4 Publications

GO - Molecular functioni

  • GTPase regulator activity Source: ProtInc

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis, Transcription, Transcription regulation

Keywords - Ligandi

Actin-binding

Enzyme and pathway databases

ReactomeiR-HSA-2029482. Regulation of actin dynamics for phagocytic cup formation.
R-HSA-203641. NOSTRIN mediated eNOS trafficking.
R-HSA-373753. Nephrin interactions.
R-HSA-3928662. EPHB-mediated forward signaling.
R-HSA-418885. DCC mediated attractive signaling.
R-HSA-5663213. RHO GTPases Activate WASPs and WAVEs.
SignaLinkiO00401.
SIGNORiO00401.

Names & Taxonomyi

Protein namesi
Recommended name:
Neural Wiskott-Aldrich syndrome protein
Short name:
N-WASP
Gene namesi
Name:WASL
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 7

Organism-specific databases

HGNCiHGNC:12735. WASL.

Subcellular locationi

  • Nucleus By similarity
  • Cytoplasm By similarity
  • Cytoplasmcytoskeleton By similarity

  • Note: Preferentially localized in the cytoplasm when phosphorylated and in the nucleus when unphosphorylated. Exported from the nucleus by an nuclear export signal (NES)-dependent mechanism to the cytoplasm.By similarity

GO - Cellular componenti

  • actin cap Source: Ensembl
  • actin cytoskeleton Source: ProtInc
  • cytoplasmic, membrane-bounded vesicle Source: BHF-UCL
  • cytosol Source: UniProtKB
  • endocytic vesicle membrane Source: Reactome
  • extracellular exosome Source: UniProtKB
  • Golgi membrane Source: Ensembl
  • lamellipodium Source: Ensembl
  • nucleus Source: UniProtKB-SubCell
  • plasma membrane Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA37346.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedCombined sources
Chaini2 – 505504Neural Wiskott-Aldrich syndrome proteinPRO_0000189000Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineCombined sources
Modified residuei242 – 2421Phosphoserine; by TNK21 Publication
Modified residuei256 – 2561Phosphotyrosine; by FAK1 and TNK2Combined sources1 Publication
Modified residuei484 – 4841PhosphoserineCombined sources
Modified residuei485 – 4851PhosphoserineCombined sources

Post-translational modificationi

Phosphorylation at Ser-242, Tyr-256, Ser-484 and Ser-485 enhances actin polymerization activity.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiO00401.
MaxQBiO00401.
PaxDbiO00401.
PeptideAtlasiO00401.
PRIDEiO00401.

PTM databases

iPTMnetiO00401.
PhosphoSiteiO00401.

Expressioni

Gene expression databases

BgeeiENSG00000106299.
CleanExiHS_WASL.
GenevisibleiO00401. HS.

Organism-specific databases

HPAiCAB005399.
HPA005750.

Interactioni

Subunit structurei

Binds actin and the Arp2/3 complex. Interacts with CDC42. Binds to SH3 domains of GRB2. Interacts with the C-terminal SH3 domain of DNMBP. Interacts with SNX9. Interacts with the WW domains of PRPF40A/FBP11. Interacts with PTK2/FAK1. Interacts with PACSIN1, PACSIN2 and PACSIN3 (By similarity). Interacts with NOSTRIN. Interacts with Shigella flexneri protein IcsA. The interaction with IcsA enhances the affinity of WASL for Arp2/3, thus assembling a tight complex which has maximal activity in actin assembly. Interacts with E.coli effector protein EspF(U). Identified in a complex containing at least WASL, BAIAP2L1 and E.coli EspF(U). Binds to TNK2. Interacts with SNX33.By similarity8 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ACTR3P611573EBI-957615,EBI-351419From a different organism.
ARPC3O151453EBI-957615,EBI-351829
CDC42P609533EBI-957615,EBI-81752
CIB1Q998287EBI-957615,EBI-372594
Fnbp1Q8R5112EBI-957615,EBI-1111424From a different organism.
GRB2P629938EBI-957615,EBI-401755
NCK1P163332EBI-957615,EBI-389883
NCK2O436393EBI-957615,EBI-713635
PTK6Q138823EBI-957615,EBI-1383632
RHOJQ9H4E53EBI-957615,EBI-6285694
RNF8O760644EBI-957615,EBI-373337
SDCBPO005603EBI-957615,EBI-727004
SNX9Q9Y5X12EBI-957615,EBI-77848
SORBS3O60504-23EBI-957615,EBI-1222956
WIPF1O435167EBI-957615,EBI-346356

Protein-protein interaction databases

BioGridi114466. 69 interactions.
DIPiDIP-29042N.
IntActiO00401. 46 interactions.
MINTiMINT-157215.
STRINGi9606.ENSP00000223023.

Structurei

Secondary structure

1
505
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni219 – 2213Combined sources
Helixi225 – 2273Combined sources
Helixi232 – 2398Combined sources
Helixi243 – 2464Combined sources
Turni249 – 2513Combined sources
Helixi252 – 26211Combined sources
Helixi265 – 2695Combined sources
Helixi435 – 4439Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2FF3X-ray2.00C451-465[»]
2LNHNMR-A207-270[»]
2VCPX-ray3.20D/E392-484[»]
4CC2X-ray1.55B/D346-357[»]
4CC7X-ray1.97B/D/F/H/J/L/N346-357[»]
ProteinModelPortaliO00401.
SMRiO00401. Positions 29-150, 207-270, 403-450, 466-491.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO00401.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini34 – 141108WH1PROSITE-ProRule annotationAdd
BLAST
Domaini203 – 21614CRIBPROSITE-ProRule annotationAdd
BLAST
Domaini405 – 42218WH2 1PROSITE-ProRule annotationAdd
BLAST
Domaini433 – 45018WH2 2PROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi277 – 392116Pro-richAdd
BLAST
Compositional biasi485 – 50521Asp-richAdd
BLAST

Sequence similaritiesi

Contains 1 CRIB domain.PROSITE-ProRule annotation
Contains 1 WH1 domain.PROSITE-ProRule annotation
Contains 2 WH2 domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG3671. Eukaryota.
ENOG4111J0D. LUCA.
GeneTreeiENSGT00730000110895.
HOGENOMiHOG000143378.
HOVERGENiHBG000222.
InParanoidiO00401.
KOiK05747.
OMAiWSKKCCG.
OrthoDBiEOG091G0VCD.
PhylomeDBiO00401.
TreeFamiTF316736.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
3.90.810.10. 1 hit.
InterProiIPR000095. CRIB_dom.
IPR030214. N-WASP.
IPR011993. PH_dom-like.
IPR011026. WASP_C.
IPR000697. WH1/EVH1_dom.
IPR003124. WH2_dom.
[Graphical view]
PANTHERiPTHR23202:SF33. PTHR23202:SF33. 1 hit.
PfamiPF00786. PBD. 1 hit.
PF00568. WH1. 1 hit.
PF02205. WH2. 2 hits.
[Graphical view]
SMARTiSM00285. PBD. 1 hit.
SM00461. WH1. 1 hit.
SM00246. WH2. 2 hits.
[Graphical view]
SUPFAMiSSF47912. SSF47912. 2 hits.
SSF50729. SSF50729. 1 hit.
PROSITEiPS50108. CRIB. 1 hit.
PS50229. WH1. 1 hit.
PS51082. WH2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O00401-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSVQQQPPP PRRVTNVGSL LLTPQENESL FTFLGKKCVT MSSAVVQLYA
60 70 80 90 100
ADRNCMWSKK CSGVACLVKD NPQRSYFLRI FDIKDGKLLW EQELYNNFVY
110 120 130 140 150
NSPRGYFHTF AGDTCQVALN FANEEEAKKF RKAVTDLLGR RQRKSEKRRD
160 170 180 190 200
PPNGPNLPMA TVDIKNPEIT TNRFYGPQVN NISHTKEKKK GKAKKKRLTK
210 220 230 240 250
ADIGTPSNFQ HIGHVGWDPN TGFDLNNLDP ELKNLFDMCG ISEAQLKDRE
260 270 280 290 300
TSKVIYDFIE KTGGVEAVKN ELRRQAPPPP PPSRGGPPPP PPPPHNSGPP
310 320 330 340 350
PPPARGRGAP PPPPSRAPTA APPPPPPSRP SVAVPPPPPN RMYPPPPPAL
360 370 380 390 400
PSSAPSGPPP PPPSVLGVGP VAPPPPPPPP PPPGPPPPPG LPSDGDHQVP
410 420 430 440 450
TTAGNKAALL DQIREGAQLK KVEQNSRPVS CSGRDALLDQ IRQGIQLKSV
460 470 480 490 500
ADGQESTPPT PAPTSGIVGA LMEVMQKRSK AIHSSDEDED EDDEEDFEDD

DEWED
Length:505
Mass (Da):54,827
Last modified:February 1, 2005 - v2
Checksum:iB5895C6055E23049
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti76 – 761Y → H in BAA20128 (PubMed:9322739).Curated
Sequence conflicti201 – 2011A → G in BAA20128 (PubMed:9322739).Curated
Sequence conflicti223 – 2231F → S in BAA20128 (PubMed:9322739).Curated
Sequence conflicti242 – 2421S → L in BAA20128 (PubMed:9322739).Curated
Sequence conflicti248 – 2481D → E in BAA20128 (PubMed:9322739).Curated
Sequence conflicti252 – 2521S → L in BAA20128 (PubMed:9322739).Curated
Sequence conflicti296 – 2961N → S in BAA20128 (PubMed:9322739).Curated
Sequence conflicti333 – 3331A → E in BAA20128 (PubMed:9322739).Curated
Sequence conflicti351 – 3511P → L in CAL26602 (Ref. 2) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D88460 mRNA. Translation: BAA20128.1.
AM295156 mRNA. Translation: CAL26602.1.
AC006333 Genomic DNA. Translation: AAQ96857.1.
BC052955 mRNA. Translation: AAH52955.1.
CCDSiCCDS34743.1.
RefSeqiNP_003932.3. NM_003941.3.
UniGeneiHs.143728.
Hs.731519.
Hs.83916.

Genome annotation databases

EnsembliENST00000223023; ENSP00000223023; ENSG00000106299.
GeneIDi8976.
KEGGihsa:8976.
UCSCiuc003vkz.5. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D88460 mRNA. Translation: BAA20128.1.
AM295156 mRNA. Translation: CAL26602.1.
AC006333 Genomic DNA. Translation: AAQ96857.1.
BC052955 mRNA. Translation: AAH52955.1.
CCDSiCCDS34743.1.
RefSeqiNP_003932.3. NM_003941.3.
UniGeneiHs.143728.
Hs.731519.
Hs.83916.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2FF3X-ray2.00C451-465[»]
2LNHNMR-A207-270[»]
2VCPX-ray3.20D/E392-484[»]
4CC2X-ray1.55B/D346-357[»]
4CC7X-ray1.97B/D/F/H/J/L/N346-357[»]
ProteinModelPortaliO00401.
SMRiO00401. Positions 29-150, 207-270, 403-450, 466-491.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114466. 69 interactions.
DIPiDIP-29042N.
IntActiO00401. 46 interactions.
MINTiMINT-157215.
STRINGi9606.ENSP00000223023.

PTM databases

iPTMnetiO00401.
PhosphoSiteiO00401.

Proteomic databases

EPDiO00401.
MaxQBiO00401.
PaxDbiO00401.
PeptideAtlasiO00401.
PRIDEiO00401.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000223023; ENSP00000223023; ENSG00000106299.
GeneIDi8976.
KEGGihsa:8976.
UCSCiuc003vkz.5. human.

Organism-specific databases

CTDi8976.
GeneCardsiWASL.
HGNCiHGNC:12735. WASL.
HPAiCAB005399.
HPA005750.
MIMi605056. gene.
neXtProtiNX_O00401.
PharmGKBiPA37346.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3671. Eukaryota.
ENOG4111J0D. LUCA.
GeneTreeiENSGT00730000110895.
HOGENOMiHOG000143378.
HOVERGENiHBG000222.
InParanoidiO00401.
KOiK05747.
OMAiWSKKCCG.
OrthoDBiEOG091G0VCD.
PhylomeDBiO00401.
TreeFamiTF316736.

Enzyme and pathway databases

ReactomeiR-HSA-2029482. Regulation of actin dynamics for phagocytic cup formation.
R-HSA-203641. NOSTRIN mediated eNOS trafficking.
R-HSA-373753. Nephrin interactions.
R-HSA-3928662. EPHB-mediated forward signaling.
R-HSA-418885. DCC mediated attractive signaling.
R-HSA-5663213. RHO GTPases Activate WASPs and WAVEs.
SignaLinkiO00401.
SIGNORiO00401.

Miscellaneous databases

ChiTaRSiWASL. human.
EvolutionaryTraceiO00401.
GeneWikiiWASL_(gene).
GenomeRNAii8976.
PROiO00401.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000106299.
CleanExiHS_WASL.
GenevisibleiO00401. HS.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
3.90.810.10. 1 hit.
InterProiIPR000095. CRIB_dom.
IPR030214. N-WASP.
IPR011993. PH_dom-like.
IPR011026. WASP_C.
IPR000697. WH1/EVH1_dom.
IPR003124. WH2_dom.
[Graphical view]
PANTHERiPTHR23202:SF33. PTHR23202:SF33. 1 hit.
PfamiPF00786. PBD. 1 hit.
PF00568. WH1. 1 hit.
PF02205. WH2. 2 hits.
[Graphical view]
SMARTiSM00285. PBD. 1 hit.
SM00461. WH1. 1 hit.
SM00246. WH2. 2 hits.
[Graphical view]
SUPFAMiSSF47912. SSF47912. 2 hits.
SSF50729. SSF50729. 1 hit.
PROSITEiPS50108. CRIB. 1 hit.
PS50229. WH1. 1 hit.
PS51082. WH2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiWASL_HUMAN
AccessioniPrimary (citable) accession number: O00401
Secondary accession number(s): A1JUI9, Q7Z746
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: February 1, 2005
Last modified: September 7, 2016
This is version 167 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.