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Protein

Dynactin subunit 6

Gene

DCTN6

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

GO - Biological processi

Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-HSA-2132295. MHC class II antigen presentation.
R-HSA-6807878. COPI-mediated anterograde transport.
R-HSA-6811436. COPI-independent Golgi-to-ER retrograde traffic.

Names & Taxonomyi

Protein namesi
Recommended name:
Dynactin subunit 6
Alternative name(s):
Dynactin subunit p27
Protein WS-3
Gene namesi
Name:DCTN6
Synonyms:WS3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 8

Organism-specific databases

HGNCiHGNC:16964. DCTN6.

Subcellular locationi

GO - Cellular componenti

  • centrosome Source: UniProtKB
  • condensed chromosome kinetochore Source: UniProtKB-SubCell
  • cytosol Source: Reactome
  • dynactin complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Cytoplasm, Cytoskeleton, Kinetochore

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134927975.

Polymorphism and mutation databases

BioMutaiDCTN6.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 190190Dynactin subunit 6PRO_0000079830Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei186 – 1861Phosphothreonine; by CDK11 Publication

Post-translational modificationi

Phosphorylation at Thr-186 by CDK1 during mitotic prometaphase creates a binding site for PLK1 that facilitates its recruitment to kinetochores.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO00399.
MaxQBiO00399.
PaxDbiO00399.
PeptideAtlasiO00399.
PRIDEiO00399.

PTM databases

iPTMnetiO00399.
PhosphoSiteiO00399.

Expressioni

Tissue specificityi

Ubiquitous.

Gene expression databases

BgeeiO00399.
ExpressionAtlasiO00399. baseline and differential.
GenevisibleiO00399. HS.

Organism-specific databases

HPAiHPA024049.
HPA024558.

Interactioni

Subunit structurei

Member of the pointed-end complex of the dynactin shoulder complex which contains DCTN4, DCTN5 and DCTN6 subunits and ACTR10. Within the complex DCTN6 forms a heterodimer with DCTN5. Interacts with PLK1.1 Publication

Protein-protein interaction databases

BioGridi115913. 43 interactions.
IntActiO00399. 12 interactions.
STRINGi9606.ENSP00000221114.

Structurei

Secondary structure

1
190
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi9 – 113Combined sources
Beta strandi21 – 299Combined sources
Beta strandi40 – 467Combined sources
Beta strandi48 – 503Combined sources
Beta strandi61 – 644Combined sources
Beta strandi82 – 843Combined sources
Beta strandi99 – 1013Combined sources
Beta strandi117 – 1193Combined sources
Beta strandi135 – 1373Combined sources
Beta strandi141 – 1455Combined sources
Beta strandi150 – 1534Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3TV0X-ray2.15A/B1-190[»]
ProteinModelPortaliO00399.
SMRiO00399. Positions 13-185.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG4042. Eukaryota.
ENOG410YETF. LUCA.
GeneTreeiENSGT00390000017890.
HOGENOMiHOG000046876.
HOVERGENiHBG051326.
InParanoidiO00399.
KOiK10428.
OMAiTIVHPKA.
OrthoDBiEOG7353Z2.
PhylomeDBiO00399.
TreeFamiTF352888.

Family and domain databases

InterProiIPR027777. DCTN6.
IPR001451. Hexapep.
IPR011004. Trimer_LpxA-like.
[Graphical view]
PANTHERiPTHR13072. PTHR13072. 1 hit.
PfamiPF00132. Hexapep. 1 hit.
[Graphical view]
SUPFAMiSSF51161. SSF51161. 1 hit.

Sequencei

Sequence statusi: Complete.

O00399-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEKTQKSVK IAPGAVVCVE SEIRGDVTIG PRTVIHPKAR IIAEAGPIVI
60 70 80 90 100
GEGNLIEEQA LIINAYPDNI TPDTEDPEPK PMIIGTNNVF EVGCYSQAMK
110 120 130 140 150
MGDNNVIESK AYVGRNVILT SGCIIGACCN LNTFEVIPEN TVIYGADCLR
160 170 180 190
RVQTERPQPQ TLQLDFLMKI LPNYHHLKKT MKGSSTPVKN
Length:190
Mass (Da):20,747
Last modified:July 1, 1997 - v1
Checksum:i7D0B12C0029C77B7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D84145 mRNA. Translation: BAA19984.1.
CR456851 mRNA. Translation: CAG33132.1.
AK314128 mRNA. Translation: BAG36818.1.
CH471080 Genomic DNA. Translation: EAW63466.1.
BC013175 mRNA. Translation: AAH13175.1.
CCDSiCCDS6076.1.
RefSeqiNP_006562.1. NM_006571.3.
UniGeneiHs.158427.
Hs.721134.

Genome annotation databases

EnsembliENST00000221114; ENSP00000221114; ENSG00000104671.
GeneIDi10671.
KEGGihsa:10671.
UCSCiuc003xhy.4. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D84145 mRNA. Translation: BAA19984.1.
CR456851 mRNA. Translation: CAG33132.1.
AK314128 mRNA. Translation: BAG36818.1.
CH471080 Genomic DNA. Translation: EAW63466.1.
BC013175 mRNA. Translation: AAH13175.1.
CCDSiCCDS6076.1.
RefSeqiNP_006562.1. NM_006571.3.
UniGeneiHs.158427.
Hs.721134.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3TV0X-ray2.15A/B1-190[»]
ProteinModelPortaliO00399.
SMRiO00399. Positions 13-185.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115913. 43 interactions.
IntActiO00399. 12 interactions.
STRINGi9606.ENSP00000221114.

PTM databases

iPTMnetiO00399.
PhosphoSiteiO00399.

Polymorphism and mutation databases

BioMutaiDCTN6.

Proteomic databases

EPDiO00399.
MaxQBiO00399.
PaxDbiO00399.
PeptideAtlasiO00399.
PRIDEiO00399.

Protocols and materials databases

DNASUi10671.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000221114; ENSP00000221114; ENSG00000104671.
GeneIDi10671.
KEGGihsa:10671.
UCSCiuc003xhy.4. human.

Organism-specific databases

CTDi10671.
GeneCardsiDCTN6.
HGNCiHGNC:16964. DCTN6.
HPAiHPA024049.
HPA024558.
MIMi612963. gene.
neXtProtiNX_O00399.
PharmGKBiPA134927975.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4042. Eukaryota.
ENOG410YETF. LUCA.
GeneTreeiENSGT00390000017890.
HOGENOMiHOG000046876.
HOVERGENiHBG051326.
InParanoidiO00399.
KOiK10428.
OMAiTIVHPKA.
OrthoDBiEOG7353Z2.
PhylomeDBiO00399.
TreeFamiTF352888.

Enzyme and pathway databases

ReactomeiR-HSA-2132295. MHC class II antigen presentation.
R-HSA-6807878. COPI-mediated anterograde transport.
R-HSA-6811436. COPI-independent Golgi-to-ER retrograde traffic.

Miscellaneous databases

GenomeRNAii10671.
PROiO00399.
SOURCEiSearch...

Gene expression databases

BgeeiO00399.
ExpressionAtlasiO00399. baseline and differential.
GenevisibleiO00399. HS.

Family and domain databases

InterProiIPR027777. DCTN6.
IPR001451. Hexapep.
IPR011004. Trimer_LpxA-like.
[Graphical view]
PANTHERiPTHR13072. PTHR13072. 1 hit.
PfamiPF00132. Hexapep. 1 hit.
[Graphical view]
SUPFAMiSSF51161. SSF51161. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Skin.
  2. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
    Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Substantia nigra.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Skin.
  6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. "Dynactin helps target Polo-like kinase 1 to kinetochores via its left-handed beta-helical p27 subunit."
    Yeh T.Y., Kowalska A.K., Scipioni B.R., Cheong F.K., Zheng M., Derewenda U., Derewenda Z.S., Schroer T.A.
    EMBO J. 32:1023-1035(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.15 ANGSTROMS), SUBUNIT, SUBCELLULAR LOCATION, PHOSPHORYLATION AT THR-186, INTERACTION WITH PLK1.

Entry informationi

Entry nameiDCTN6_HUMAN
AccessioniPrimary (citable) accession number: O00399
Secondary accession number(s): B2RAC1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 1, 1997
Last modified: July 6, 2016
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.