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O00341 (EAA5_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 117. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Excitatory amino acid transporter 5
Alternative name(s):
Retinal glutamate transporter
Solute carrier family 1 member 7
Gene names
Name:SLC1A7
Synonyms:EAAT5
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length560 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Transports L-glutamate; the L-glutamate uptake is sodium- and voltage-dependent and chloride-independent. Its associated chloride conductance may participate in visual processing.

Subunit structure

Interacts with the PDZ domains of DLG4.

Subcellular location

Membrane; Multi-pass membrane protein.

Tissue specificity

Expressed primarily in retina. Detectable in liver, heart, muscle and brain.

Sequence similarities

Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A7 subfamily. [View classification]

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 560560Excitatory amino acid transporter 5
PRO_0000202073

Regions

Topological domain1 – 1616Cytoplasmic Potential
Transmembrane17 – 3721Helical; Potential
Transmembrane60 – 8021Helical; Potential
Transmembrane94 – 11421Helical; Potential
Topological domain115 – 216102Extracellular Potential
Transmembrane217 – 23721Helical; Potential
Transmembrane260 – 28021Helical; Potential
Transmembrane300 – 32021Helical; Potential
Transmembrane330 – 35021Helical; Potential
Transmembrane372 – 39221Helical; Potential
Transmembrane414 – 43421Helical; Potential
Transmembrane457 – 47721Helical; Potential

Amino acid modifications

Glycosylation1911N-linked (GlcNAc...) Potential

Natural variations

Natural variant411R → C in a colorectal cancer sample; somatic mutation. Ref.5
VAR_035707
Natural variant5371Q → R. Ref.1 Ref.3 Ref.4
Corresponds to variant rs1288401 [ dbSNP | Ensembl ].
VAR_052488

Experimental info

Sequence conflict51A → T in AAB53971. Ref.1
Sequence conflict701V → F in AAB53971. Ref.1
Sequence conflict2441A → G in AAB53971. Ref.1
Sequence conflict4121A → G in AAB53971. Ref.1
Sequence conflict4491A → G in AAB53971. Ref.1

Sequences

Sequence LengthMass (Da)Tools
O00341 [UniParc].

Last modified September 23, 2008. Version 2.
Checksum: 17C527B745BB01E6

FASTA56060,658
        10         20         30         40         50         60 
MVPHAILARG RDVCRRNGLL ILSVLSVIVG CLLGFFLRTR RLSPQEISYF QFPGELLMRM 

        70         80         90        100        110        120 
LKMMILPLVV SSLMSGLASL DAKTSSRLGV LTVAYYLWTT FMAVIVGIFM VSIIHPGSAA 

       130        140        150        160        170        180 
QKETTEQSGK PIMSSADALL DLIRNMFPAN LVEATFKQYR TKTTPVVKSP KVAPEEAPPR 

       190        200        210        220        230        240 
RILIYGVQEE NGSHVQNFAL DLTPPPEVVY KSEPGTSDGM NVLGIVFFSA TMGIMLGRMG 

       250        260        270        280        290        300 
DSGAPLVSFC QCLNESVMKI VAVAVWYFPF GIVFLIAGKI LEMDDPRAVG KKLGFYSVTV 

       310        320        330        340        350        360 
VCGLVLHGLF ILPLLYFFIT KKNPIVFIRG ILQALLIALA TSSSSATLPI TFKCLLENNH 

       370        380        390        400        410        420 
IDRRIARFVL PVGATINMDG TALYEAVAAI FIAQVNNYEL DFGQIITISI TATAASIGAA 

       430        440        450        460        470        480 
GIPQAGLVTM VIVLTSVGLP TDDITLIIAV DWALDRFRTM INVLGDALAA GIMAHICRKD 

       490        500        510        520        530        540 
FARDTGTEKL LPCETKPVSL QEIVAAQQNG CVKSVAEASE LTLGPTCPHH VPVQVEQDEE 

       550        560 
LPAASLNHCT IQISELETNV 

« Hide

References

« Hide 'large scale' references
[1]"Excitatory amino acid transporter 5, a retinal glutamate transporter coupled to a chloride conductance."
Arriza J.L., Eliasof S., Kavanaugh M.P., Amara S.G.
Proc. Natl. Acad. Sci. U.S.A. 94:4155-4160(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT ARG-537.
Tissue: Retina.
[2]"The DNA sequence and biological annotation of human chromosome 1."
Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. expand/collapse author list , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT ARG-537.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ARG-537.
Tissue: Eye.
[5]"The consensus coding sequences of human breast and colorectal cancers."
Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. expand/collapse author list , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E.
Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANT [LARGE SCALE ANALYSIS] CYS-41.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U76362 mRNA. Translation: AAB53971.1.
AL445183 Genomic DNA. Translation: CAH72594.1.
CH471059 Genomic DNA. Translation: EAX06755.1.
BC012119 mRNA. Translation: AAH12119.1.
BC017242 mRNA. Translation: AAH17242.1.
RefSeqNP_001274526.1. NM_001287597.1.
NP_006662.3. NM_006671.5.
UniGeneHs.104637.

3D structure databases

ProteinModelPortalO00341.
SMRO00341. Positions 19-474.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid112403. 2 interactions.
IntActO00341. 1 interaction.
STRING9606.ENSP00000360549.

Chemistry

BindingDBO00341.
ChEMBLCHEMBL4390.
DrugBankDB00142. L-Glutamic Acid.
GuidetoPHARMACOLOGY872.

Protein family/group databases

TCDB2.A.23.2.5. the dicarboxylate/amino acid:cation (na(+) or h(+)) symporter (daacs) family.

PTM databases

PhosphoSiteO00341.

Proteomic databases

PaxDbO00341.
PRIDEO00341.

Protocols and materials databases

DNASU6512.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000371494; ENSP00000360549; ENSG00000162383.
GeneID6512.
KEGGhsa:6512.
UCSCuc001cuy.3. human.

Organism-specific databases

CTD6512.
GeneCardsGC01M053552.
H-InvDBHIX0000592.
HIX0159667.
HGNCHGNC:10945. SLC1A7.
HPAHPA049124.
MIM604471. gene.
neXtProtNX_O00341.
PharmGKBPA35832.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG1301.
HOGENOMHOG000208776.
HOVERGENHBG000080.
InParanoidO00341.
KOK05618.
OMAGFSIVRF.
OrthoDBEOG7RV9G2.
PhylomeDBO00341.
TreeFamTF315206.

Enzyme and pathway databases

ReactomeREACT_13685. Neuronal System.
REACT_15518. Transmembrane transport of small molecules.
REACT_19419. Amino acid and oligopeptide SLC transporters.

Gene expression databases

ArrayExpressO00341.
BgeeO00341.
CleanExHS_SLC1A7.
GenevestigatorO00341.

Family and domain databases

Gene3D1.10.3860.10. 2 hits.
InterProIPR001991. Na-dicarboxylate_symporter.
IPR018107. Na-dicarboxylate_symporter_CS.
[Graphical view]
PANTHERPTHR11958. PTHR11958. 1 hit.
PfamPF00375. SDF. 1 hit.
[Graphical view]
PRINTSPR00173. EDTRNSPORT.
SUPFAMSSF118215. SSF118215. 2 hits.
PROSITEPS00713. NA_DICARBOXYL_SYMP_1. 1 hit.
PS00714. NA_DICARBOXYL_SYMP_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiExcitatory_amino-acid_transporter_5.
GenomeRNAi6512.
NextBio25323.
PROO00341.
SOURCESearch...

Entry information

Entry nameEAA5_HUMAN
AccessionPrimary (citable) accession number: O00341
Secondary accession number(s): Q5VVZ0, Q969Z8
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: September 23, 2008
Last modified: April 16, 2014
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 1

Human chromosome 1: entries, gene names and cross-references to MIM