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Protein

Excitatory amino acid transporter 5

Gene

SLC1A7

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Transports L-glutamate; the L-glutamate uptake is sodium- and voltage-dependent and chloride-independent. Its associated chloride conductance may participate in visual processing.

GO - Molecular functioni

  1. L-glutamate transmembrane transporter activity Source: UniProtKB
  2. sodium:dicarboxylate symporter activity Source: InterPro

GO - Biological processi

  1. dicarboxylic acid transport Source: UniProtKB
  2. ion transport Source: Reactome
  3. L-glutamate transmembrane transport Source: GOC
  4. transmembrane transport Source: Reactome
Complete GO annotation...

Keywords - Biological processi

Symport, Transport

Enzyme and pathway databases

ReactomeiREACT_12591. Glutamate Neurotransmitter Release Cycle.
REACT_19397. Transport of inorganic cations/anions and amino acids/oligopeptides.

Protein family/group databases

TCDBi2.A.23.2.5. the dicarboxylate/amino acid:cation (na(+) or h(+)) symporter (daacs) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Excitatory amino acid transporter 5
Alternative name(s):
Retinal glutamate transporter
Solute carrier family 1 member 7
Gene namesi
Name:SLC1A7
Synonyms:EAAT5
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 1

Organism-specific databases

HGNCiHGNC:10945. SLC1A7.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1616CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei17 – 3721HelicalSequence AnalysisAdd
BLAST
Transmembranei60 – 8021HelicalSequence AnalysisAdd
BLAST
Transmembranei94 – 11421HelicalSequence AnalysisAdd
BLAST
Topological domaini115 – 216102ExtracellularSequence AnalysisAdd
BLAST
Transmembranei217 – 23721HelicalSequence AnalysisAdd
BLAST
Transmembranei260 – 28021HelicalSequence AnalysisAdd
BLAST
Transmembranei300 – 32021HelicalSequence AnalysisAdd
BLAST
Transmembranei330 – 35021HelicalSequence AnalysisAdd
BLAST
Transmembranei372 – 39221HelicalSequence AnalysisAdd
BLAST
Transmembranei414 – 43421HelicalSequence AnalysisAdd
BLAST
Transmembranei457 – 47721HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA35832.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 560560Excitatory amino acid transporter 5PRO_0000202073Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi191 – 1911N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiO00341.
PRIDEiO00341.

PTM databases

PhosphoSiteiO00341.

Expressioni

Tissue specificityi

Expressed primarily in retina. Detectable in liver, heart, muscle and brain.

Gene expression databases

BgeeiO00341.
CleanExiHS_SLC1A7.
ExpressionAtlasiO00341. baseline and differential.
GenevestigatoriO00341.

Organism-specific databases

HPAiHPA049124.

Interactioni

Subunit structurei

Interacts with the PDZ domains of DLG4.

Protein-protein interaction databases

BioGridi112403. 2 interactions.
IntActiO00341. 1 interaction.
STRINGi9606.ENSP00000360549.

Structurei

3D structure databases

ProteinModelPortaliO00341.
SMRiO00341. Positions 19-474.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1301.
GeneTreeiENSGT00760000119117.
HOGENOMiHOG000208776.
HOVERGENiHBG000080.
InParanoidiO00341.
KOiK05618.
OMAiIYKSEPG.
OrthoDBiEOG7RV9G2.
PhylomeDBiO00341.
TreeFamiTF315206.

Family and domain databases

Gene3Di1.10.3860.10. 2 hits.
InterProiIPR001991. Na-dicarboxylate_symporter.
IPR018107. Na-dicarboxylate_symporter_CS.
[Graphical view]
PANTHERiPTHR11958. PTHR11958. 1 hit.
PfamiPF00375. SDF. 1 hit.
[Graphical view]
PRINTSiPR00173. EDTRNSPORT.
SUPFAMiSSF118215. SSF118215. 2 hits.
PROSITEiPS00713. NA_DICARBOXYL_SYMP_1. 1 hit.
PS00714. NA_DICARBOXYL_SYMP_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O00341-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVPHAILARG RDVCRRNGLL ILSVLSVIVG CLLGFFLRTR RLSPQEISYF
60 70 80 90 100
QFPGELLMRM LKMMILPLVV SSLMSGLASL DAKTSSRLGV LTVAYYLWTT
110 120 130 140 150
FMAVIVGIFM VSIIHPGSAA QKETTEQSGK PIMSSADALL DLIRNMFPAN
160 170 180 190 200
LVEATFKQYR TKTTPVVKSP KVAPEEAPPR RILIYGVQEE NGSHVQNFAL
210 220 230 240 250
DLTPPPEVVY KSEPGTSDGM NVLGIVFFSA TMGIMLGRMG DSGAPLVSFC
260 270 280 290 300
QCLNESVMKI VAVAVWYFPF GIVFLIAGKI LEMDDPRAVG KKLGFYSVTV
310 320 330 340 350
VCGLVLHGLF ILPLLYFFIT KKNPIVFIRG ILQALLIALA TSSSSATLPI
360 370 380 390 400
TFKCLLENNH IDRRIARFVL PVGATINMDG TALYEAVAAI FIAQVNNYEL
410 420 430 440 450
DFGQIITISI TATAASIGAA GIPQAGLVTM VIVLTSVGLP TDDITLIIAV
460 470 480 490 500
DWALDRFRTM INVLGDALAA GIMAHICRKD FARDTGTEKL LPCETKPVSL
510 520 530 540 550
QEIVAAQQNG CVKSVAEASE LTLGPTCPHH VPVQVEQDEE LPAASLNHCT
560
IQISELETNV
Length:560
Mass (Da):60,658
Last modified:September 23, 2008 - v2
Checksum:i17C527B745BB01E6
GO
Isoform 2 (identifier: O00341-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     145-158: NMFPANLVEATFKQ → QKEESWRNGPKGPG
     159-560: Missing.

Note: No experimental confirmation available.

Show »
Length:158
Mass (Da):17,383
Checksum:i8C1A9A4905368917
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti5 – 51A → T in AAB53971 (PubMed:9108121).Curated
Sequence conflicti70 – 701V → F in AAB53971 (PubMed:9108121).Curated
Sequence conflicti244 – 2441A → G in AAB53971 (PubMed:9108121).Curated
Sequence conflicti412 – 4121A → G in AAB53971 (PubMed:9108121).Curated
Sequence conflicti449 – 4491A → G in AAB53971 (PubMed:9108121).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti41 – 411R → C in a colorectal cancer sample; somatic mutation. 1 Publication
VAR_035707
Natural varianti537 – 5371Q → R.3 Publications
Corresponds to variant rs1288401 [ dbSNP | Ensembl ].
VAR_052488

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei145 – 15814NMFPA…ATFKQ → QKEESWRNGPKGPG in isoform 2. 1 PublicationVSP_056560Add
BLAST
Alternative sequencei159 – 560402Missing in isoform 2. 1 PublicationVSP_056561Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U76362 mRNA. Translation: AAB53971.1.
AL445183 Genomic DNA. Translation: CAH72594.1.
CH471059 Genomic DNA. Translation: EAX06754.1.
CH471059 Genomic DNA. Translation: EAX06755.1.
BC000651 mRNA. Translation: AAH00651.1.
BC012119 mRNA. Translation: AAH12119.1.
BC017242 mRNA. Translation: AAH17242.1.
CCDSiCCDS574.1. [O00341-1]
CCDS72798.1. [O00341-2]
RefSeqiNP_001274525.1. NM_001287596.1. [O00341-2]
NP_001274526.1. NM_001287597.1.
NP_006662.3. NM_006671.5. [O00341-1]
UniGeneiHs.104637.

Genome annotation databases

EnsembliENST00000371491; ENSP00000360546; ENSG00000162383. [O00341-2]
ENST00000371494; ENSP00000360549; ENSG00000162383. [O00341-1]
GeneIDi6512.
KEGGihsa:6512.
UCSCiuc001cuy.3. human. [O00341-1]
uc001cuz.4. human.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U76362 mRNA. Translation: AAB53971.1.
AL445183 Genomic DNA. Translation: CAH72594.1.
CH471059 Genomic DNA. Translation: EAX06754.1.
CH471059 Genomic DNA. Translation: EAX06755.1.
BC000651 mRNA. Translation: AAH00651.1.
BC012119 mRNA. Translation: AAH12119.1.
BC017242 mRNA. Translation: AAH17242.1.
CCDSiCCDS574.1. [O00341-1]
CCDS72798.1. [O00341-2]
RefSeqiNP_001274525.1. NM_001287596.1. [O00341-2]
NP_001274526.1. NM_001287597.1.
NP_006662.3. NM_006671.5. [O00341-1]
UniGeneiHs.104637.

3D structure databases

ProteinModelPortaliO00341.
SMRiO00341. Positions 19-474.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi112403. 2 interactions.
IntActiO00341. 1 interaction.
STRINGi9606.ENSP00000360549.

Chemistry

ChEMBLiCHEMBL4390.
GuidetoPHARMACOLOGYi872.

Protein family/group databases

TCDBi2.A.23.2.5. the dicarboxylate/amino acid:cation (na(+) or h(+)) symporter (daacs) family.

PTM databases

PhosphoSiteiO00341.

Proteomic databases

PaxDbiO00341.
PRIDEiO00341.

Protocols and materials databases

DNASUi6512.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000371491; ENSP00000360546; ENSG00000162383. [O00341-2]
ENST00000371494; ENSP00000360549; ENSG00000162383. [O00341-1]
GeneIDi6512.
KEGGihsa:6512.
UCSCiuc001cuy.3. human. [O00341-1]
uc001cuz.4. human.

Organism-specific databases

CTDi6512.
GeneCardsiGC01M053552.
H-InvDBHIX0000592.
HIX0159667.
HGNCiHGNC:10945. SLC1A7.
HPAiHPA049124.
MIMi604471. gene.
neXtProtiNX_O00341.
PharmGKBiPA35832.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG1301.
GeneTreeiENSGT00760000119117.
HOGENOMiHOG000208776.
HOVERGENiHBG000080.
InParanoidiO00341.
KOiK05618.
OMAiIYKSEPG.
OrthoDBiEOG7RV9G2.
PhylomeDBiO00341.
TreeFamiTF315206.

Enzyme and pathway databases

ReactomeiREACT_12591. Glutamate Neurotransmitter Release Cycle.
REACT_19397. Transport of inorganic cations/anions and amino acids/oligopeptides.

Miscellaneous databases

GeneWikiiExcitatory_amino-acid_transporter_5.
GenomeRNAii6512.
NextBioi25323.
PROiO00341.
SOURCEiSearch...

Gene expression databases

BgeeiO00341.
CleanExiHS_SLC1A7.
ExpressionAtlasiO00341. baseline and differential.
GenevestigatoriO00341.

Family and domain databases

Gene3Di1.10.3860.10. 2 hits.
InterProiIPR001991. Na-dicarboxylate_symporter.
IPR018107. Na-dicarboxylate_symporter_CS.
[Graphical view]
PANTHERiPTHR11958. PTHR11958. 1 hit.
PfamiPF00375. SDF. 1 hit.
[Graphical view]
PRINTSiPR00173. EDTRNSPORT.
SUPFAMiSSF118215. SSF118215. 2 hits.
PROSITEiPS00713. NA_DICARBOXYL_SYMP_1. 1 hit.
PS00714. NA_DICARBOXYL_SYMP_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Excitatory amino acid transporter 5, a retinal glutamate transporter coupled to a chloride conductance."
    Arriza J.L., Eliasof S., Kavanaugh M.P., Amara S.G.
    Proc. Natl. Acad. Sci. U.S.A. 94:4155-4160(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT ARG-537.
    Tissue: Retina.
  2. "The DNA sequence and biological annotation of human chromosome 1."
    Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.
    , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
    Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT ARG-537.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2), VARIANT ARG-537.
    Tissue: Eye.
  5. Cited for: VARIANT [LARGE SCALE ANALYSIS] CYS-41.

Entry informationi

Entry nameiEAA5_HUMAN
AccessioniPrimary (citable) accession number: O00341
Secondary accession number(s): Q5VVZ0, Q969Z8, Q9BW45
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: September 23, 2008
Last modified: January 7, 2015
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.