O00341 (EAA5_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Excitatory amino acid transporter 5

Alternative name(s):
Retinal glutamate transporter
Solute carrier family 1 member 7

Gene names

Name:	SLC1A7
Synonyms:	EAAT5

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	560 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Transports L-glutamate; the L-glutamate uptake is sodium- and voltage-dependent and chloride-independent. Its associated chloride conductance may participate in visual processing.
Subunit structure	Interacts with the PDZ domains of DLG4.
Subcellular location	Membrane; Multi-pass membrane protein.
Tissue specificity	Expressed primarily in retina. Detectable in liver, heart, muscle and brain.
Sequence similarities	Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A7 subfamily. [View classification]

Ontologies

Keywords
Biological process	Symport Transport
Cellular component	Membrane
Coding sequence diversity	Polymorphism
Domain	Transmembrane Transmembrane helix
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	integral to membrane Non-traceable author statement. Source: UniProtKB plasma membrane Traceable author statement. Source: Reactome
Molecular_function	L-glutamate transmembrane transporter activity Traceable author statement Ref.1. Source: UniProtKB sodium:dicarboxylate symporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 560	560	Excitatory amino acid transporter 5		PRO_0000202073
Regions
	Topological domain	1 – 16	16	Cytoplasmic Potential
	Transmembrane	17 – 37	21	Helical; Potential
	Transmembrane	60 – 80	21	Helical; Potential
	Transmembrane	94 – 114	21	Helical; Potential
	Topological domain	115 – 216	102	Extracellular Potential
	Transmembrane	217 – 237	21	Helical; Potential
	Transmembrane	260 – 280	21	Helical; Potential
	Transmembrane	300 – 320	21	Helical; Potential
	Transmembrane	330 – 350	21	Helical; Potential
	Transmembrane	372 – 392	21	Helical; Potential
	Transmembrane	414 – 434	21	Helical; Potential
	Transmembrane	457 – 477	21	Helical; Potential
Amino acid modifications
	Glycosylation	191	1	N-linked (GlcNAc...) Potential
Natural variations
	Natural variant	41	1	R → C in a colorectal cancer sample; somatic mutation. Ref.5		VAR_035707
	Natural variant	537	1	Q → R. Ref.1 Ref.3 Ref.4 Corresponds to variant rs1288401 [ dbSNP | Ensembl ].		VAR_052488
Experimental info
	Sequence conflict	5	1	A → T in AAB53971. Ref.1
	Sequence conflict	70	1	V → F in AAB53971. Ref.1
	Sequence conflict	244	1	A → G in AAB53971. Ref.1
	Sequence conflict	412	1	A → G in AAB53971. Ref.1
	Sequence conflict	449	1	A → G in AAB53971. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O00341 [UniParc]. Last modified September 23, 2008. Version 2. Checksum: 17C527B745BB01E6	FASTA	560	60,658
	10 20 30 40 50 60 MVPHAILARG RDVCRRNGLL ILSVLSVIVG CLLGFFLRTR RLSPQEISYF QFPGELLMRM 70 80 90 100 110 120 LKMMILPLVV SSLMSGLASL DAKTSSRLGV LTVAYYLWTT FMAVIVGIFM VSIIHPGSAA 130 140 150 160 170 180 QKETTEQSGK PIMSSADALL DLIRNMFPAN LVEATFKQYR TKTTPVVKSP KVAPEEAPPR 190 200 210 220 230 240 RILIYGVQEE NGSHVQNFAL DLTPPPEVVY KSEPGTSDGM NVLGIVFFSA TMGIMLGRMG 250 260 270 280 290 300 DSGAPLVSFC QCLNESVMKI VAVAVWYFPF GIVFLIAGKI LEMDDPRAVG KKLGFYSVTV 310 320 330 340 350 360 VCGLVLHGLF ILPLLYFFIT KKNPIVFIRG ILQALLIALA TSSSSATLPI TFKCLLENNH 370 380 390 400 410 420 IDRRIARFVL PVGATINMDG TALYEAVAAI FIAQVNNYEL DFGQIITISI TATAASIGAA 430 440 450 460 470 480 GIPQAGLVTM VIVLTSVGLP TDDITLIIAV DWALDRFRTM INVLGDALAA GIMAHICRKD 490 500 510 520 530 540 FARDTGTEKL LPCETKPVSL QEIVAAQQNG CVKSVAEASE LTLGPTCPHH VPVQVEQDEE 550 560 LPAASLNHCT IQISELETNV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Excitatory amino acid transporter 5, a retinal glutamate transporter coupled to a chloride conductance." Arriza J.L., Eliasof S., Kavanaugh M.P., Amara S.G. Proc. Natl. Acad. Sci. U.S.A. 94:4155-4160(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT ARG-537. Tissue: Retina.
[2]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT ARG-537.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ARG-537. Tissue: Eye.
[5]	"The consensus coding sequences of human breast and colorectal cancers." Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E. Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] CYS-41.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U76362 mRNA. Translation: AAB53971.1. AL445183 Genomic DNA. Translation: CAH72594.1. CH471059 Genomic DNA. Translation: EAX06755.1. BC012119 mRNA. Translation: AAH12119.1. BC017242 mRNA. Translation: AAH17242.1.
IPI	IPI00298441.
RefSeq	NP_006662.3. NM_006671.4.
UniGene	Hs.104637.
3D structure databases
ProteinModelPortal	O00341.
ModBase	Search...
Protein-protein interaction databases
IntAct	O00341. 1 interaction.
STRING	9606.ENSP00000360549.
PTM databases
PhosphoSite	O00341.
Proteomic databases
PaxDb	O00341.
PRIDE	O00341.
Protocols and materials databases
DNASU	6512.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000371494; ENSP00000360549; ENSG00000162383.
GeneID	6512.
KEGG	hsa:6512.
UCSC	uc001cuy.3. human.
Organism-specific databases
CTD	6512.
GeneCards	GC01M053552.
H-InvDB	HIX0000592. HIX0159667.
HGNC	HGNC:10945. SLC1A7.
HPA	HPA049124.
MIM	604471. gene.
neXtProt	NX_O00341.
PharmGKB	PA35832.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG1301.
HOGENOM	HOG000208776.
HOVERGEN	HBG000080.
InParanoid	O00341.
KO	K05618.
OMA	ESTTHRI.
OrthoDB	EOG4FR0RG.
Enzyme and pathway databases
Reactome	REACT_13685. Neuronal System. REACT_15518. Transmembrane transport of small molecules. REACT_19419. Amino acid and oligopeptide SLC transporters.
Gene expression databases
ArrayExpress	O00341.
Bgee	O00341.
CleanEx	HS_SLC1A7.
Genevestigator	O00341.
GermOnline	ENSG00000162383. Homo sapiens.
Family and domain databases
InterPro	IPR001991. Na-dicarboxylate_symporter. IPR018107. Na-dicarboxylate_symporter_CS. [Graphical view]
PANTHER	PTHR11958. PTHR11958. 1 hit.
Pfam	PF00375. SDF. 1 hit. [Graphical view]
PRINTS	PR00173. EDTRNSPORT.
PROSITE	PS00713. NA_DICARBOXYL_SYMP_1. 1 hit. PS00714. NA_DICARBOXYL_SYMP_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	O00341.
ChEMBL	CHEMBL4390.
DrugBank	DB00142. L-Glutamic Acid.
GenomeRNAi	6512.
NextBio	25323.
SOURCE	Search...

Entry information

Entry name

EAA5_HUMAN

Accession

Primary (citable) accession number: O00341
Secondary accession number(s): Q5VVZ0, Q969Z8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	September 23, 2008
Last modified:	May 1, 2013
This is version 107 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.