Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Excitatory amino acid transporter 5

Gene

SLC1A7

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Transports L-glutamate; the L-glutamate uptake is sodium- and voltage-dependent and chloride-independent. Its associated chloride conductance may participate in visual processing.

GO - Molecular functioni

GO - Biological processi

  • dicarboxylic acid transport Source: UniProtKB
  • glutamate secretion Source: Reactome
  • ion transport Source: Reactome
Complete GO annotation...

Keywords - Biological processi

Symport, Transport

Enzyme and pathway databases

BioCyciZFISH:ENSG00000162383-MONOMER.
ReactomeiR-HSA-210500. Glutamate Neurotransmitter Release Cycle.
R-HSA-425393. Transport of inorganic cations/anions and amino acids/oligopeptides.

Protein family/group databases

TCDBi2.A.23.2.5. the dicarboxylate/amino acid:cation (na(+) or h(+)) symporter (daacs) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Excitatory amino acid transporter 5
Alternative name(s):
Retinal glutamate transporter
Solute carrier family 1 member 7
Gene namesi
Name:SLC1A7
Synonyms:EAAT5
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:10945. SLC1A7.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 16CytoplasmicSequence analysisAdd BLAST16
Transmembranei17 – 37HelicalSequence analysisAdd BLAST21
Transmembranei60 – 80HelicalSequence analysisAdd BLAST21
Transmembranei94 – 114HelicalSequence analysisAdd BLAST21
Topological domaini115 – 216ExtracellularSequence analysisAdd BLAST102
Transmembranei217 – 237HelicalSequence analysisAdd BLAST21
Transmembranei260 – 280HelicalSequence analysisAdd BLAST21
Transmembranei300 – 320HelicalSequence analysisAdd BLAST21
Transmembranei330 – 350HelicalSequence analysisAdd BLAST21
Transmembranei372 – 392HelicalSequence analysisAdd BLAST21
Transmembranei414 – 434HelicalSequence analysisAdd BLAST21
Transmembranei457 – 477HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi6512.
OpenTargetsiENSG00000162383.
PharmGKBiPA35832.

Chemistry databases

GuidetoPHARMACOLOGYi872.

Polymorphism and mutation databases

BioMutaiSLC1A7.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002020731 – 560Excitatory amino acid transporter 5Add BLAST560

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi191N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiO00341.
PeptideAtlasiO00341.
PRIDEiO00341.

PTM databases

iPTMnetiO00341.
PhosphoSitePlusiO00341.

Expressioni

Tissue specificityi

Expressed primarily in retina. Detectable in liver, heart, muscle and brain.

Gene expression databases

BgeeiENSG00000162383.
CleanExiHS_SLC1A7.
ExpressionAtlasiO00341. baseline and differential.
GenevisibleiO00341. HS.

Organism-specific databases

HPAiHPA049124.

Interactioni

Subunit structurei

Interacts with the PDZ domains of DLG4.

Protein-protein interaction databases

BioGridi112403. 2 interactors.
IntActiO00341. 1 interactor.
STRINGi9606.ENSP00000360549.

Structurei

3D structure databases

ProteinModelPortaliO00341.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3787. Eukaryota.
COG1301. LUCA.
GeneTreeiENSGT00760000119117.
HOGENOMiHOG000208776.
HOVERGENiHBG000080.
InParanoidiO00341.
KOiK05618.
PhylomeDBiO00341.
TreeFamiTF315206.

Family and domain databases

Gene3Di1.10.3860.10. 2 hits.
InterProiIPR001991. Na-dicarboxylate_symporter.
IPR018107. Na-dicarboxylate_symporter_CS.
[Graphical view]
PANTHERiPTHR11958. PTHR11958. 3 hits.
PfamiPF00375. SDF. 1 hit.
[Graphical view]
PRINTSiPR00173. EDTRNSPORT.
SUPFAMiSSF118215. SSF118215. 2 hits.
PROSITEiPS00713. NA_DICARBOXYL_SYMP_1. 1 hit.
PS00714. NA_DICARBOXYL_SYMP_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O00341-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVPHAILARG RDVCRRNGLL ILSVLSVIVG CLLGFFLRTR RLSPQEISYF
60 70 80 90 100
QFPGELLMRM LKMMILPLVV SSLMSGLASL DAKTSSRLGV LTVAYYLWTT
110 120 130 140 150
FMAVIVGIFM VSIIHPGSAA QKETTEQSGK PIMSSADALL DLIRNMFPAN
160 170 180 190 200
LVEATFKQYR TKTTPVVKSP KVAPEEAPPR RILIYGVQEE NGSHVQNFAL
210 220 230 240 250
DLTPPPEVVY KSEPGTSDGM NVLGIVFFSA TMGIMLGRMG DSGAPLVSFC
260 270 280 290 300
QCLNESVMKI VAVAVWYFPF GIVFLIAGKI LEMDDPRAVG KKLGFYSVTV
310 320 330 340 350
VCGLVLHGLF ILPLLYFFIT KKNPIVFIRG ILQALLIALA TSSSSATLPI
360 370 380 390 400
TFKCLLENNH IDRRIARFVL PVGATINMDG TALYEAVAAI FIAQVNNYEL
410 420 430 440 450
DFGQIITISI TATAASIGAA GIPQAGLVTM VIVLTSVGLP TDDITLIIAV
460 470 480 490 500
DWALDRFRTM INVLGDALAA GIMAHICRKD FARDTGTEKL LPCETKPVSL
510 520 530 540 550
QEIVAAQQNG CVKSVAEASE LTLGPTCPHH VPVQVEQDEE LPAASLNHCT
560
IQISELETNV
Length:560
Mass (Da):60,658
Last modified:September 23, 2008 - v2
Checksum:i17C527B745BB01E6
GO
Isoform 2 (identifier: O00341-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     145-158: NMFPANLVEATFKQ → QKEESWRNGPKGPG
     159-560: Missing.

Note: No experimental confirmation available.
Show »
Length:158
Mass (Da):17,383
Checksum:i8C1A9A4905368917
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti5A → T in AAB53971 (PubMed:9108121).Curated1
Sequence conflicti70V → F in AAB53971 (PubMed:9108121).Curated1
Sequence conflicti244A → G in AAB53971 (PubMed:9108121).Curated1
Sequence conflicti412A → G in AAB53971 (PubMed:9108121).Curated1
Sequence conflicti449A → G in AAB53971 (PubMed:9108121).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_03570741R → C in a colorectal cancer sample; somatic mutation. 1 PublicationCorresponds to variant rs375136400dbSNPEnsembl.1
Natural variantiVAR_052488537Q → R.3 PublicationsCorresponds to variant rs1288401dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_056560145 – 158NMFPA…ATFKQ → QKEESWRNGPKGPG in isoform 2. 1 PublicationAdd BLAST14
Alternative sequenceiVSP_056561159 – 560Missing in isoform 2. 1 PublicationAdd BLAST402

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U76362 mRNA. Translation: AAB53971.1.
AL445183 Genomic DNA. Translation: CAH72594.1.
CH471059 Genomic DNA. Translation: EAX06754.1.
CH471059 Genomic DNA. Translation: EAX06755.1.
BC000651 mRNA. Translation: AAH00651.1.
BC012119 mRNA. Translation: AAH12119.1.
BC017242 mRNA. Translation: AAH17242.1.
CCDSiCCDS574.1. [O00341-1]
CCDS72798.1. [O00341-2]
RefSeqiNP_001274525.1. NM_001287596.1. [O00341-2]
NP_001274526.1. NM_001287597.1.
NP_006662.3. NM_006671.5. [O00341-1]
UniGeneiHs.104637.

Genome annotation databases

EnsembliENST00000371491; ENSP00000360546; ENSG00000162383. [O00341-2]
ENST00000371494; ENSP00000360549; ENSG00000162383. [O00341-1]
GeneIDi6512.
KEGGihsa:6512.
UCSCiuc001cuy.5. human. [O00341-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U76362 mRNA. Translation: AAB53971.1.
AL445183 Genomic DNA. Translation: CAH72594.1.
CH471059 Genomic DNA. Translation: EAX06754.1.
CH471059 Genomic DNA. Translation: EAX06755.1.
BC000651 mRNA. Translation: AAH00651.1.
BC012119 mRNA. Translation: AAH12119.1.
BC017242 mRNA. Translation: AAH17242.1.
CCDSiCCDS574.1. [O00341-1]
CCDS72798.1. [O00341-2]
RefSeqiNP_001274525.1. NM_001287596.1. [O00341-2]
NP_001274526.1. NM_001287597.1.
NP_006662.3. NM_006671.5. [O00341-1]
UniGeneiHs.104637.

3D structure databases

ProteinModelPortaliO00341.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi112403. 2 interactors.
IntActiO00341. 1 interactor.
STRINGi9606.ENSP00000360549.

Chemistry databases

GuidetoPHARMACOLOGYi872.

Protein family/group databases

TCDBi2.A.23.2.5. the dicarboxylate/amino acid:cation (na(+) or h(+)) symporter (daacs) family.

PTM databases

iPTMnetiO00341.
PhosphoSitePlusiO00341.

Polymorphism and mutation databases

BioMutaiSLC1A7.

Proteomic databases

PaxDbiO00341.
PeptideAtlasiO00341.
PRIDEiO00341.

Protocols and materials databases

DNASUi6512.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000371491; ENSP00000360546; ENSG00000162383. [O00341-2]
ENST00000371494; ENSP00000360549; ENSG00000162383. [O00341-1]
GeneIDi6512.
KEGGihsa:6512.
UCSCiuc001cuy.5. human. [O00341-1]

Organism-specific databases

CTDi6512.
DisGeNETi6512.
GeneCardsiSLC1A7.
H-InvDBHIX0000592.
HIX0159667.
HGNCiHGNC:10945. SLC1A7.
HPAiHPA049124.
MIMi604471. gene.
neXtProtiNX_O00341.
OpenTargetsiENSG00000162383.
PharmGKBiPA35832.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3787. Eukaryota.
COG1301. LUCA.
GeneTreeiENSGT00760000119117.
HOGENOMiHOG000208776.
HOVERGENiHBG000080.
InParanoidiO00341.
KOiK05618.
PhylomeDBiO00341.
TreeFamiTF315206.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000162383-MONOMER.
ReactomeiR-HSA-210500. Glutamate Neurotransmitter Release Cycle.
R-HSA-425393. Transport of inorganic cations/anions and amino acids/oligopeptides.

Miscellaneous databases

GeneWikiiExcitatory_amino-acid_transporter_5.
GenomeRNAii6512.
PROiO00341.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000162383.
CleanExiHS_SLC1A7.
ExpressionAtlasiO00341. baseline and differential.
GenevisibleiO00341. HS.

Family and domain databases

Gene3Di1.10.3860.10. 2 hits.
InterProiIPR001991. Na-dicarboxylate_symporter.
IPR018107. Na-dicarboxylate_symporter_CS.
[Graphical view]
PANTHERiPTHR11958. PTHR11958. 3 hits.
PfamiPF00375. SDF. 1 hit.
[Graphical view]
PRINTSiPR00173. EDTRNSPORT.
SUPFAMiSSF118215. SSF118215. 2 hits.
PROSITEiPS00713. NA_DICARBOXYL_SYMP_1. 1 hit.
PS00714. NA_DICARBOXYL_SYMP_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEAA5_HUMAN
AccessioniPrimary (citable) accession number: O00341
Secondary accession number(s): Q5VVZ0, Q969Z8, Q9BW45
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: September 23, 2008
Last modified: November 30, 2016
This is version 139 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.