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O00341

- EAA5_HUMAN

UniProt

O00341 - EAA5_HUMAN

Protein

Excitatory amino acid transporter 5

Gene

SLC1A7

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 121 (01 Oct 2014)
      Sequence version 2 (23 Sep 2008)
      Previous versions | rss
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    Functioni

    Transports L-glutamate; the L-glutamate uptake is sodium- and voltage-dependent and chloride-independent. Its associated chloride conductance may participate in visual processing.

    GO - Molecular functioni

    1. L-glutamate transmembrane transporter activity Source: UniProtKB
    2. sodium:dicarboxylate symporter activity Source: InterPro

    GO - Biological processi

    1. dicarboxylic acid transport Source: UniProtKB
    2. ion transport Source: Reactome
    3. L-glutamate transport Source: GOC
    4. transmembrane transport Source: Reactome

    Keywords - Biological processi

    Symport, Transport

    Enzyme and pathway databases

    ReactomeiREACT_12591. Glutamate Neurotransmitter Release Cycle.
    REACT_19397. Transport of inorganic cations/anions and amino acids/oligopeptides.

    Protein family/group databases

    TCDBi2.A.23.2.5. the dicarboxylate/amino acid:cation (na(+) or h(+)) symporter (daacs) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Excitatory amino acid transporter 5
    Alternative name(s):
    Retinal glutamate transporter
    Solute carrier family 1 member 7
    Gene namesi
    Name:SLC1A7
    Synonyms:EAAT5
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 1

    Organism-specific databases

    HGNCiHGNC:10945. SLC1A7.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: Reactome

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA35832.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 560560Excitatory amino acid transporter 5PRO_0000202073Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi191 – 1911N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiO00341.
    PRIDEiO00341.

    PTM databases

    PhosphoSiteiO00341.

    Expressioni

    Tissue specificityi

    Expressed primarily in retina. Detectable in liver, heart, muscle and brain.

    Gene expression databases

    ArrayExpressiO00341.
    BgeeiO00341.
    CleanExiHS_SLC1A7.
    GenevestigatoriO00341.

    Organism-specific databases

    HPAiHPA049124.

    Interactioni

    Subunit structurei

    Interacts with the PDZ domains of DLG4.

    Protein-protein interaction databases

    BioGridi112403. 2 interactions.
    IntActiO00341. 1 interaction.
    STRINGi9606.ENSP00000360549.

    Structurei

    3D structure databases

    ProteinModelPortaliO00341.
    SMRiO00341. Positions 19-474.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 1616CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini115 – 216102ExtracellularSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei17 – 3721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei60 – 8021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei94 – 11421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei217 – 23721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei260 – 28021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei300 – 32021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei330 – 35021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei372 – 39221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei414 – 43421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei457 – 47721HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG1301.
    HOGENOMiHOG000208776.
    HOVERGENiHBG000080.
    InParanoidiO00341.
    KOiK05618.
    OMAiKLLPCET.
    OrthoDBiEOG7RV9G2.
    PhylomeDBiO00341.
    TreeFamiTF315206.

    Family and domain databases

    Gene3Di1.10.3860.10. 2 hits.
    InterProiIPR001991. Na-dicarboxylate_symporter.
    IPR018107. Na-dicarboxylate_symporter_CS.
    [Graphical view]
    PANTHERiPTHR11958. PTHR11958. 1 hit.
    PfamiPF00375. SDF. 1 hit.
    [Graphical view]
    PRINTSiPR00173. EDTRNSPORT.
    SUPFAMiSSF118215. SSF118215. 2 hits.
    PROSITEiPS00713. NA_DICARBOXYL_SYMP_1. 1 hit.
    PS00714. NA_DICARBOXYL_SYMP_2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: O00341-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MVPHAILARG RDVCRRNGLL ILSVLSVIVG CLLGFFLRTR RLSPQEISYF    50
    QFPGELLMRM LKMMILPLVV SSLMSGLASL DAKTSSRLGV LTVAYYLWTT 100
    FMAVIVGIFM VSIIHPGSAA QKETTEQSGK PIMSSADALL DLIRNMFPAN 150
    LVEATFKQYR TKTTPVVKSP KVAPEEAPPR RILIYGVQEE NGSHVQNFAL 200
    DLTPPPEVVY KSEPGTSDGM NVLGIVFFSA TMGIMLGRMG DSGAPLVSFC 250
    QCLNESVMKI VAVAVWYFPF GIVFLIAGKI LEMDDPRAVG KKLGFYSVTV 300
    VCGLVLHGLF ILPLLYFFIT KKNPIVFIRG ILQALLIALA TSSSSATLPI 350
    TFKCLLENNH IDRRIARFVL PVGATINMDG TALYEAVAAI FIAQVNNYEL 400
    DFGQIITISI TATAASIGAA GIPQAGLVTM VIVLTSVGLP TDDITLIIAV 450
    DWALDRFRTM INVLGDALAA GIMAHICRKD FARDTGTEKL LPCETKPVSL 500
    QEIVAAQQNG CVKSVAEASE LTLGPTCPHH VPVQVEQDEE LPAASLNHCT 550
    IQISELETNV 560
    Length:560
    Mass (Da):60,658
    Last modified:September 23, 2008 - v2
    Checksum:i17C527B745BB01E6
    GO
    Isoform 2 (identifier: O00341-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         145-158: NMFPANLVEATFKQ → QKEESWRNGPKGPG
         159-560: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:158
    Mass (Da):17,383
    Checksum:i8C1A9A4905368917
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti5 – 51A → T in AAB53971. (PubMed:9108121)Curated
    Sequence conflicti70 – 701V → F in AAB53971. (PubMed:9108121)Curated
    Sequence conflicti244 – 2441A → G in AAB53971. (PubMed:9108121)Curated
    Sequence conflicti412 – 4121A → G in AAB53971. (PubMed:9108121)Curated
    Sequence conflicti449 – 4491A → G in AAB53971. (PubMed:9108121)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti41 – 411R → C in a colorectal cancer sample; somatic mutation. 1 Publication
    VAR_035707
    Natural varianti537 – 5371Q → R.3 Publications
    Corresponds to variant rs1288401 [ dbSNP | Ensembl ].
    VAR_052488

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei145 – 15814NMFPA…ATFKQ → QKEESWRNGPKGPG in isoform 2. 1 PublicationVSP_056560Add
    BLAST
    Alternative sequencei159 – 560402Missing in isoform 2. 1 PublicationVSP_056561Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U76362 mRNA. Translation: AAB53971.1.
    AL445183 Genomic DNA. Translation: CAH72594.1.
    CH471059 Genomic DNA. Translation: EAX06754.1.
    CH471059 Genomic DNA. Translation: EAX06755.1.
    BC000651 mRNA. Translation: AAH00651.1.
    BC012119 mRNA. Translation: AAH12119.1.
    BC017242 mRNA. Translation: AAH17242.1.
    CCDSiCCDS574.1.
    RefSeqiNP_001274525.1. NM_001287596.1.
    NP_001274526.1. NM_001287597.1.
    NP_006662.3. NM_006671.5.
    UniGeneiHs.104637.

    Genome annotation databases

    EnsembliENST00000371491; ENSP00000360546; ENSG00000162383.
    ENST00000371494; ENSP00000360549; ENSG00000162383.
    GeneIDi6512.
    KEGGihsa:6512.
    UCSCiuc001cuy.3. human.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U76362 mRNA. Translation: AAB53971.1 .
    AL445183 Genomic DNA. Translation: CAH72594.1 .
    CH471059 Genomic DNA. Translation: EAX06754.1 .
    CH471059 Genomic DNA. Translation: EAX06755.1 .
    BC000651 mRNA. Translation: AAH00651.1 .
    BC012119 mRNA. Translation: AAH12119.1 .
    BC017242 mRNA. Translation: AAH17242.1 .
    CCDSi CCDS574.1.
    RefSeqi NP_001274525.1. NM_001287596.1.
    NP_001274526.1. NM_001287597.1.
    NP_006662.3. NM_006671.5.
    UniGenei Hs.104637.

    3D structure databases

    ProteinModelPortali O00341.
    SMRi O00341. Positions 19-474.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 112403. 2 interactions.
    IntActi O00341. 1 interaction.
    STRINGi 9606.ENSP00000360549.

    Chemistry

    BindingDBi O00341.
    ChEMBLi CHEMBL4390.
    DrugBanki DB00142. L-Glutamic Acid.
    GuidetoPHARMACOLOGYi 872.

    Protein family/group databases

    TCDBi 2.A.23.2.5. the dicarboxylate/amino acid:cation (na(+) or h(+)) symporter (daacs) family.

    PTM databases

    PhosphoSitei O00341.

    Proteomic databases

    PaxDbi O00341.
    PRIDEi O00341.

    Protocols and materials databases

    DNASUi 6512.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000371491 ; ENSP00000360546 ; ENSG00000162383 .
    ENST00000371494 ; ENSP00000360549 ; ENSG00000162383 .
    GeneIDi 6512.
    KEGGi hsa:6512.
    UCSCi uc001cuy.3. human.

    Organism-specific databases

    CTDi 6512.
    GeneCardsi GC01M053552.
    H-InvDB HIX0000592.
    HIX0159667.
    HGNCi HGNC:10945. SLC1A7.
    HPAi HPA049124.
    MIMi 604471. gene.
    neXtProti NX_O00341.
    PharmGKBi PA35832.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG1301.
    HOGENOMi HOG000208776.
    HOVERGENi HBG000080.
    InParanoidi O00341.
    KOi K05618.
    OMAi KLLPCET.
    OrthoDBi EOG7RV9G2.
    PhylomeDBi O00341.
    TreeFami TF315206.

    Enzyme and pathway databases

    Reactomei REACT_12591. Glutamate Neurotransmitter Release Cycle.
    REACT_19397. Transport of inorganic cations/anions and amino acids/oligopeptides.

    Miscellaneous databases

    GeneWikii Excitatory_amino-acid_transporter_5.
    GenomeRNAii 6512.
    NextBioi 25323.
    PROi O00341.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O00341.
    Bgeei O00341.
    CleanExi HS_SLC1A7.
    Genevestigatori O00341.

    Family and domain databases

    Gene3Di 1.10.3860.10. 2 hits.
    InterProi IPR001991. Na-dicarboxylate_symporter.
    IPR018107. Na-dicarboxylate_symporter_CS.
    [Graphical view ]
    PANTHERi PTHR11958. PTHR11958. 1 hit.
    Pfami PF00375. SDF. 1 hit.
    [Graphical view ]
    PRINTSi PR00173. EDTRNSPORT.
    SUPFAMi SSF118215. SSF118215. 2 hits.
    PROSITEi PS00713. NA_DICARBOXYL_SYMP_1. 1 hit.
    PS00714. NA_DICARBOXYL_SYMP_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Excitatory amino acid transporter 5, a retinal glutamate transporter coupled to a chloride conductance."
      Arriza J.L., Eliasof S., Kavanaugh M.P., Amara S.G.
      Proc. Natl. Acad. Sci. U.S.A. 94:4155-4160(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT ARG-537.
      Tissue: Retina.
    2. "The DNA sequence and biological annotation of human chromosome 1."
      Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.
      , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
      Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT ARG-537.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2), VARIANT ARG-537.
      Tissue: Eye.
    5. Cited for: VARIANT [LARGE SCALE ANALYSIS] CYS-41.

    Entry informationi

    Entry nameiEAA5_HUMAN
    AccessioniPrimary (citable) accession number: O00341
    Secondary accession number(s): Q5VVZ0, Q969Z8, Q9BW45
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: September 23, 2008
    Last modified: October 1, 2014
    This is version 121 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 1
      Human chromosome 1: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3