Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Sodium/nucleoside cotransporter 1

Gene

SLC28A1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Sodium-dependent and pyrimidine-selective. Exhibits the transport characteristics of the nucleoside transport system cit or N2 subtype (N2/cit) (selective for pyrimidine nucleosides and adenosine). It also transports the antiviral pyrimidine nucleoside analogs 3'-azido-3'-deoxythymidine (AZT) and 2',3'-dideoxycytidine (ddC). It may be involved in the intestinal absorption and renal handling of pyrimidine nucleoside analogs used to treat acquired immunodeficiency syndrome (AIDS). It has the following selective inhibition: adenosine, thymidine, cytidine, uridine >> guanosine, inosine.

GO - Molecular functioni

GO - Biological processi

  • nucleobase-containing compound metabolic process Source: ProtInc
  • nucleoside transport Source: ProtInc
Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

BioCyciZFISH:ENSG00000156222-MONOMER.
ReactomeiR-HSA-83936. Transport of nucleosides and free purine and pyrimidine bases across the plasma membrane.

Protein family/group databases

TCDBi2.A.41.2.3. the concentrative nucleoside transporter (cnt) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium/nucleoside cotransporter 1
Alternative name(s):
Concentrative nucleoside transporter 1
Short name:
CNT 1
Short name:
hCNT1
Na(+)/nucleoside cotransporter 1
Sodium-coupled nucleoside transporter 1
Solute carrier family 28 member 1
Gene namesi
Name:SLC28A1
Synonyms:CNT1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 15

Organism-specific databases

HGNCiHGNC:11001. SLC28A1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 80CytoplasmicSequence analysisAdd BLAST80
Transmembranei81 – 104HelicalSequence analysisAdd BLAST24
Topological domaini105 – 109ExtracellularSequence analysis5
Transmembranei110 – 128HelicalSequence analysisAdd BLAST19
Topological domaini129 – 147CytoplasmicSequence analysisAdd BLAST19
Transmembranei148 – 167HelicalSequence analysisAdd BLAST20
Topological domaini168 – 178ExtracellularSequence analysisAdd BLAST11
Transmembranei179 – 195HelicalSequence analysisAdd BLAST17
Topological domaini196 – 201CytoplasmicSequence analysis6
Transmembranei202 – 222HelicalSequence analysisAdd BLAST21
Topological domaini223 – 261ExtracellularSequence analysisAdd BLAST39
Transmembranei262 – 283HelicalSequence analysisAdd BLAST22
Topological domaini284 – 294CytoplasmicSequence analysisAdd BLAST11
Transmembranei295 – 318HelicalSequence analysisAdd BLAST24
Topological domaini319 – 337ExtracellularSequence analysisAdd BLAST19
Transmembranei338 – 360HelicalSequence analysisAdd BLAST23
Topological domaini361 – 366CytoplasmicSequence analysis6
Transmembranei367 – 386HelicalSequence analysisAdd BLAST20
Topological domaini387 – 423ExtracellularSequence analysisAdd BLAST37
Transmembranei424 – 446HelicalSequence analysisAdd BLAST23
Topological domaini447 – 457CytoplasmicSequence analysisAdd BLAST11
Transmembranei458 – 479HelicalSequence analysisAdd BLAST22
Topological domaini480 – 534ExtracellularSequence analysisAdd BLAST55
Transmembranei535 – 558HelicalSequence analysisAdd BLAST24
Topological domaini559 – 569CytoplasmicSequence analysisAdd BLAST11
Transmembranei570 – 592HelicalSequence analysisAdd BLAST23
Topological domaini593 – 649ExtracellularSequence analysisAdd BLAST57

GO - Cellular componenti

  • integral component of plasma membrane Source: ProtInc
  • membrane Source: ProtInc
  • plasma membrane Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi318S → G: Enables transport of purine nucleosides. 1 Publication1
Mutagenesisi319Q → M: Increases transport of purine nucleosides; when associated with Gly-319. 1 Publication1
Mutagenesisi352S → T: Produces a full purine-type transporter; when associated with Gly-319 and Met-320. 1 Publication1

Organism-specific databases

DisGeNETi9154.
OpenTargetsiENSG00000156222.
PharmGKBiPA387.

Chemistry databases

ChEMBLiCHEMBL5551.
DrugBankiDB00441. Gemcitabine.
DB00649. Stavudine.
DB00495. Zidovudine.

Polymorphism and mutation databases

BioMutaiSLC28A1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000704461 – 649Sodium/nucleoside cotransporter 1Add BLAST649

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi605N-linked (GlcNAc...)Sequence analysis1
Glycosylationi643N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

EPDiO00337.
PaxDbiO00337.
PeptideAtlasiO00337.
PRIDEiO00337.

PTM databases

iPTMnetiO00337.
PhosphoSitePlusiO00337.

Expressioni

Tissue specificityi

Expressed in kidney.

Gene expression databases

BgeeiENSG00000156222.
CleanExiHS_SLC28A1.
ExpressionAtlasiO00337. baseline and differential.
GenevisibleiO00337. HS.

Organism-specific databases

HPAiHPA019551.

Interactioni

Protein-protein interaction databases

STRINGi9606.ENSP00000286749.

Chemistry databases

BindingDBiO00337.

Structurei

3D structure databases

ProteinModelPortaliO00337.
SMRiO00337.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi609 – 612Poly-Ser4

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3747. Eukaryota.
COG1972. LUCA.
GeneTreeiENSGT00390000016025.
HOVERGENiHBG054073.
InParanoidiO00337.
KOiK11536.
OMAiSEICAIM.
OrthoDBiEOG091G05D5.
PhylomeDBiO00337.
TreeFamiTF314131.

Family and domain databases

InterProiIPR008276. C_nuclsd_transpt.
IPR018270. C_nuclsd_transpt_met_bac.
IPR011657. CNT_C_dom.
IPR002668. CNT_N_dom.
IPR011642. Gate_dom.
[Graphical view]
PANTHERiPTHR10590. PTHR10590. 1 hit.
PfamiPF07670. Gate. 1 hit.
PF07662. Nucleos_tra2_C. 1 hit.
PF01773. Nucleos_tra2_N. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00804. nupC. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O00337-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MENDPSRRRE SISLTPVAKG LENMGADFLE SLEEGQLPRS DLSPAEIRSS
60 70 80 90 100
WSEAAPKPFS RWRNLQPALR ARSFCREHMQ LFRWIGTGLL CTGLSAFLLV
110 120 130 140 150
ACLLDFQRAL ALFVLTCVVL TFLGHRLLKR LLGPKLRRFL KPQGHPRLLL
160 170 180 190 200
WFKRGLALAA FLGLVLWLSL DTSQRPEQLV SFAGICVFVA LLFACSKHHC
210 220 230 240 250
AVSWRAVSWG LGLQFVLGLL VIRTEPGFIA FEWLGEQIRI FLSYTKAGSS
260 270 280 290 300
FVFGEALVKD VFAFQVLPII VFFSCVISVL YHVGLMQWVI LKIAWLMQVT
310 320 330 340 350
MGTTATETLS VAGNIFVSQT EAPLLIRPYL ADMTLSEVHV VMTGGYATIA
360 370 380 390 400
GSLLGAYISF GIDATSLIAA SVMAAPCALA LSKLVYPEVE ESKFRREEGV
410 420 430 440 450
KLTYGDAQNL IEAASTGAAI SVKVVANIAA NLIAFLAVLD FINAALSWLG
460 470 480 490 500
DMVDIQGLSF QLICSYILRP VAFLMGVAWE DCPVVAELLG IKLFLNEFVA
510 520 530 540 550
YQDLSKYKQR RLAGAEEWVG DRKQWISVRA EVLTTFALCG FANFSSIGIM
560 570 580 590 600
LGGLTSMVPQ RKSDFSQIVL RALFTGACVS LVNACMAGIL YMPRGAEVDC
610 620 630 640
MSLLNTTLSS SSFEIYQCCR EAFQSVNPEF SPEALDNCCR FYNHTICAQ
Length:649
Mass (Da):71,584
Last modified:May 5, 2009 - v2
Checksum:i42E952443B46B102
GO
Isoform 2 (identifier: O00337-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     155-175: GLALAAFLGLVLWLSLDTSQR → DPRPWSKEGPNQYLPQITWTV
     176-649: Missing.

Show »
Length:175
Mass (Da):20,180
Checksum:i34C7DD93D84F60EE
GO

Polymorphismi

Three variant forms of isoform 1 (A, B and C) are expressed in the kidney. All three variant forms have similar nucleoside transport activity.

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_00949934E → G in allele A. 2 Publications1
Natural variantiVAR_009500140L → LV in allele A. 2 Publications1
Natural variantiVAR_009501189V → I in allele A. 2 PublicationsCorresponds to variant rs2290272dbSNPEnsembl.1
Natural variantiVAR_057194190A → S.Corresponds to variant rs45523532dbSNPEnsembl.1
Natural variantiVAR_057195237Q → K.Corresponds to variant rs8187758dbSNPEnsembl.1
Natural variantiVAR_009502409N → S in allele B. 1 Publication1
Natural variantiVAR_057196510R → C.1 PublicationCorresponds to variant rs2242047dbSNPEnsembl.1
Natural variantiVAR_009503521D → N in alleles B and C. 2 PublicationsCorresponds to variant rs2242046dbSNPEnsembl.1
Natural variantiVAR_061802546S → P.Corresponds to variant rs45584739dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_037221155 – 175GLALA…DTSQR → DPRPWSKEGPNQYLPQITWT V in isoform 2. 1 PublicationAdd BLAST21
Alternative sequenceiVSP_037222176 – 649Missing in isoform 2. 1 PublicationAdd BLAST474

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U62966 mRNA. Translation: AAB53837.1.
U62967 mRNA. Translation: AAB53838.1.
U62968 mRNA. Translation: AAB53839.1.
AF187978
, AF187969, AF187970, AF187971, AF187972, AF187973, AF187974, AF187975, AF187976, AF187977 Genomic DNA. Translation: AAF15353.1.
AK291997 mRNA. Translation: BAF84686.1.
AC087468 Genomic DNA. No translation available.
AC103741 Genomic DNA. No translation available.
BC029788 mRNA. Translation: AAH29788.1.
BC039898 mRNA. Translation: AAH39898.1.
BC126204 mRNA. Translation: AAI26205.1.
BC126206 mRNA. Translation: AAI26207.1.
CCDSiCCDS10334.1. [O00337-1]
CCDS10335.1. [O00337-2]
RefSeqiNP_001274691.1. NM_001287762.1. [O00337-1]
NP_004204.3. NM_004213.4. [O00337-1]
NP_964014.1. NM_201651.2. [O00337-2]
UniGeneiHs.459187.

Genome annotation databases

EnsembliENST00000286749; ENSP00000286749; ENSG00000156222. [O00337-1]
ENST00000338602; ENSP00000341629; ENSG00000156222. [O00337-2]
ENST00000394573; ENSP00000378074; ENSG00000156222. [O00337-1]
GeneIDi9154.
KEGGihsa:9154.
UCSCiuc002blf.5. human. [O00337-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U62966 mRNA. Translation: AAB53837.1.
U62967 mRNA. Translation: AAB53838.1.
U62968 mRNA. Translation: AAB53839.1.
AF187978
, AF187969, AF187970, AF187971, AF187972, AF187973, AF187974, AF187975, AF187976, AF187977 Genomic DNA. Translation: AAF15353.1.
AK291997 mRNA. Translation: BAF84686.1.
AC087468 Genomic DNA. No translation available.
AC103741 Genomic DNA. No translation available.
BC029788 mRNA. Translation: AAH29788.1.
BC039898 mRNA. Translation: AAH39898.1.
BC126204 mRNA. Translation: AAI26205.1.
BC126206 mRNA. Translation: AAI26207.1.
CCDSiCCDS10334.1. [O00337-1]
CCDS10335.1. [O00337-2]
RefSeqiNP_001274691.1. NM_001287762.1. [O00337-1]
NP_004204.3. NM_004213.4. [O00337-1]
NP_964014.1. NM_201651.2. [O00337-2]
UniGeneiHs.459187.

3D structure databases

ProteinModelPortaliO00337.
SMRiO00337.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9606.ENSP00000286749.

Chemistry databases

BindingDBiO00337.
ChEMBLiCHEMBL5551.
DrugBankiDB00441. Gemcitabine.
DB00649. Stavudine.
DB00495. Zidovudine.

Protein family/group databases

TCDBi2.A.41.2.3. the concentrative nucleoside transporter (cnt) family.

PTM databases

iPTMnetiO00337.
PhosphoSitePlusiO00337.

Polymorphism and mutation databases

BioMutaiSLC28A1.

Proteomic databases

EPDiO00337.
PaxDbiO00337.
PeptideAtlasiO00337.
PRIDEiO00337.

Protocols and materials databases

DNASUi9154.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000286749; ENSP00000286749; ENSG00000156222. [O00337-1]
ENST00000338602; ENSP00000341629; ENSG00000156222. [O00337-2]
ENST00000394573; ENSP00000378074; ENSG00000156222. [O00337-1]
GeneIDi9154.
KEGGihsa:9154.
UCSCiuc002blf.5. human. [O00337-1]

Organism-specific databases

CTDi9154.
DisGeNETi9154.
GeneCardsiSLC28A1.
HGNCiHGNC:11001. SLC28A1.
HPAiHPA019551.
MIMi606207. gene.
neXtProtiNX_O00337.
OpenTargetsiENSG00000156222.
PharmGKBiPA387.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3747. Eukaryota.
COG1972. LUCA.
GeneTreeiENSGT00390000016025.
HOVERGENiHBG054073.
InParanoidiO00337.
KOiK11536.
OMAiSEICAIM.
OrthoDBiEOG091G05D5.
PhylomeDBiO00337.
TreeFamiTF314131.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000156222-MONOMER.
ReactomeiR-HSA-83936. Transport of nucleosides and free purine and pyrimidine bases across the plasma membrane.

Miscellaneous databases

ChiTaRSiSLC28A1. human.
GeneWikiiConcentrative_nucleoside_transporter_1.
GenomeRNAii9154.
PROiO00337.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000156222.
CleanExiHS_SLC28A1.
ExpressionAtlasiO00337. baseline and differential.
GenevisibleiO00337. HS.

Family and domain databases

InterProiIPR008276. C_nuclsd_transpt.
IPR018270. C_nuclsd_transpt_met_bac.
IPR011657. CNT_C_dom.
IPR002668. CNT_N_dom.
IPR011642. Gate_dom.
[Graphical view]
PANTHERiPTHR10590. PTHR10590. 1 hit.
PfamiPF07670. Gate. 1 hit.
PF07662. Nucleos_tra2_C. 1 hit.
PF01773. Nucleos_tra2_N. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00804. nupC. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiS28A1_HUMAN
AccessioniPrimary (citable) accession number: O00337
Secondary accession number(s): A0AV42
, A8K7I2, O00335, O00336, Q5U5S6, Q5U648, Q9UEZ9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 5, 2009
Last modified: November 2, 2016
This is version 146 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 15
    Human chromosome 15: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.